NO05G01060, NO05G01060 (gene) Nannochloropsis oceanica | NanDeSyn: Nannochloropsis Design & Synthesis Initiative

Overview


Name	NO05G01060
Unique Name	NO05G01060
Type	gene
Organism	Nannochloropsis oceanica (N. oceanica IMET1)
Sequence length	2848
Alignment location	chr5:313798..316645 -

Link to JBrowse

Properties


Property Name	Value
Description	Cytochrome b5

Mutants

Expression

Analysis:

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Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
chr5	genome	chr5:313798..316645 -

Analyses

This gene is derived from or has results from the following analyses


Analysis Name	Date Performed
GSE178672	2023-12-15
PRJNA933693	2023-10-26
PRJNA943449	2023-07-19
PXD016054	2021-01-14
PXD016699	2021-01-08
GSE149904	2020-10-10
NoIMET1_WT_HS	2020-09-29
GSE139615	2020-03-11
PRJNA241382	2020-03-11
BLASTP analysis of N. oceanica IMET1 genes	2019-07-11
InterPro analysis for N. oceanica IMET1 genes	2019-07-11
GO annotation for IMET1v2 genes	2019-07-10
PXD008721	2019-04-30
PRJNA182180	2019-04-25
Gene prediction for N. oceanica IMET1	2017-10-24

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR036400	Cyt_B5-like_heme/steroid_sf
IPR001199	Cyt_B5-like_heme/steroid-bd

Vocabulary: **Cellular Component**
Term	Definition
GO:0016020	membrane

Vocabulary: **Molecular Function**
Term	Definition
GO:0005488	binding

Homology

[Close]

BLAST of NO05G01060 vs. NCBI_GenBank
Match: EWM28245.1 (cytochrome b5 [Nannochloropsis gaditana])

HSP 1 Score: 255.4 bits (651), Expect = 1.000e-64
Identity = 125/130 (96.15%), Postives = 130/130 (100.00%), Query Frame = 0

Query:    1 MAPKQYTLDEVAGHNTEKDIWLIIGNEKTGGPKVYDVTKYLEEHPGGSEVLLDHAGKYADEMFEDIGHSGDAKEKLKTLLVGELTPEDVEKLASEKVSRASELPSKGGLNPFAVVVLLVAIVLGLYLSKQ 131
            MAP+Q+TL+EVAGHN+EKDIWLIIGNEKTGGPKVYDVTKYLEEHPGGSEVLLDHAGKYADEMFEDIGHSGDAKEKLKTL+VGELTPEDVEKLASEKVSRASELPSKGGLNPFAVVVLLVAIVLGLYLSKQ
Sbjct:    1 MAPQQFTLEEVAGHNSEKDIWLIIGNEKTGGPKVYDVTKYLEEHPGGSEVLLDHAGKYADEMFEDIGHSGDAKEKLKTLMVGELTPEDVEKLASEKVSRASELPSKGGLNPFAVVVLLVAIVLGLYLSKQ 130

[Close]

BLAST of NO05G01060 vs. NCBI_GenBank
Match: GAX27651.1 (hypothetical protein FisN_13Hh257 [Fistulifera solaris])

HSP 1 Score: 130.2 bits (326), Expect = 4.800e-27
Identity = 67/131 (51.15%), Postives = 92/131 (70.23%), Query Frame = 0

Query:    4 KQYTLDEVAGHNTEKDIWLIIGNEKTGGPKVYDVTKYLEEHPGGSEVLLDHAGKYADEMFEDIGHSGDAKEKLKTLLVGELTPEDVEKLASEKVSRASELPSK--GGLNPFAVVVL--LVAIVLGLYLSKQ 131
            K+YTL+E+A HNT++D WLIIGN   GGPKVYDVTKYL++HPGG+EVLLD AG+ ADE FEDIGHS +A+++L   L+G      ++  A EK+  A+E  SK  GG+    +V L  +V + +  Y ++Q
Sbjct:    5 KEYTLEEIAKHNTQEDCWLIIGNASNGGPKVYDVTKYLDDHPGGAEVLLDVAGQDADEFFEDIGHSKEARKELSKYLIGNF---KLDAAAMEKMKAAAEKRSKSEGGMVSTLLVALIAIVGVAVAYYKTQQ 132

[Close]

BLAST of NO05G01060 vs. NCBI_GenBank
Match: GAX27191.1 (hypothetical protein FisN_13Lh257 [Fistulifera solaris])

HSP 1 Score: 128.3 bits (321), Expect = 1.800e-26
Identity = 66/131 (50.38%), Postives = 91/131 (69.47%), Query Frame = 0

Query:    4 KQYTLDEVAGHNTEKDIWLIIGNEKTGGPKVYDVTKYLEEHPGGSEVLLDHAGKYADEMFEDIGHSGDAKEKLKTLLVGELTPEDVEKLASEKVSRASELPSK--GGLNPFAVVVL--LVAIVLGLYLSKQ 131
            K+YTL+E+  HNT++D WLIIGN   GGPKVYDVTKYL++HPGG+EVLLD AG+ ADE FEDIGHS +A+++L   L+G      ++  A EK+  A+E  SK  GG+    +V L  +V + +  Y ++Q
Sbjct:    5 KEYTLEEIVKHNTQEDCWLIIGNASNGGPKVYDVTKYLDDHPGGAEVLLDVAGQDADEFFEDIGHSKEARKELSKYLIGNF---KLDAAALEKMKAAAEKRSKNEGGMVSTLLVALIAIVGVAVAYYKTQQ 132

[Close]

BLAST of NO05G01060 vs. NCBI_GenBank
Match: OEU12251.1 (cytochrome b5 [Fragilariopsis cylindrus CCMP1102])

HSP 1 Score: 124.4 bits (311), Expect = 2.700e-25
Identity = 58/99 (58.59%), Postives = 77/99 (77.78%), Query Frame = 0

Query:    4 KQYTLDEVAGHNTEKDIWLIIGNEKTGGPKVYDVTKYLEEHPGGSEVLLDHAGKYADEMFEDIGHSGDAKEKLKTLLVG--ELTPEDVEKLASEKVSRA 101
            K+YT  EV+ H  E+D W+IIGNE  GG KVYDV+KYL++HPGG+EV+LD +G+ ADE FEDIGHSGDA+++LK  L+G  +L+ E++EKL  E   RA
Sbjct:   10 KEYTSAEVSKHTLEEDCWMIIGNESNGGSKVYDVSKYLDDHPGGAEVMLDVSGQNADEFFEDIGHSGDARKELKKYLIGTYKLSEEEIEKLKIEAEKRA 108

[Close]

BLAST of NO05G01060 vs. NCBI_GenBank
Match: CBJ26085.1 (microsomal cytochrome b5 [Ectocarpus siliculosus])

HSP 1 Score: 120.2 bits (300), Expect = 5.000e-24
Identity = 62/130 (47.69%), Postives = 88/130 (67.69%), Query Frame = 0

Query:    5 QYTLDEVAGHNTEKDIWLIIGNEKTGG-PKVYDVTKYLEEHPGGSEVLLDHAGKYADEMFEDIGHSGDAKEKLKTLLVG--ELTPEDVEKLASEKVSRASELPSK-GGLNPFAVVVLLVAIVLGLYLSKQ 131
            +Y++ EVA H T++  WL+I +   G  PKVYDV+ YL +HPGG+EV+++ AG+ A  MFEDIGHS DA+ ++K   +G  +LT E+  K+A E   +A     + GGLNP AV+VLL+AI +G Y S Q
Sbjct:    9 EYSMTEVATHTTKESTWLVIKDMNDGDTPKVYDVSSYLNDHPGGAEVMMEVAGQDATNMFEDIGHSSDARTEMKKFQIGLLKLTDEEKAKIAQEAAKKAERASRQGGGLNPLAVLVLLLAISVGYYYSNQ 138

[Close]

BLAST of NO05G01060 vs. NCBI_GenBank
Match: AAM27441.1 (microsomal cytochrome b5 [Phaeodactylum tricornutum])

HSP 1 Score: 119.4 bits (298), Expect = 8.600e-24
Identity = 53/81 (65.43%), Postives = 65/81 (80.25%), Query Frame = 0

Query:    2 APKQYTLDEVAGHNTEKDIWLIIGNEKTGGPKVYDVTKYLEEHPGGSEVLLDHAGKYADEMFEDIGHSGDAKEKLKTLLVG 83
            A K+YTLDE++ H T +  WLIIGN   GGPKVYDVTKYL++HPGG+EV+LD AG+ ADE FEDIGHS +A+ +LK  LVG
Sbjct:    3 AEKEYTLDEISQHTTTESCWLIIGNASNGGPKVYDVTKYLDDHPGGAEVMLDVAGQDADEFFEDIGHSKEARAELKNYLVG 83

[Close]

BLAST of NO05G01060 vs. NCBI_GenBank
Match: XP_002184806.1 (predicted protein [Phaeodactylum tricornutum CCAP 1055/1] >EEC43865.1 predicted protein [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 117.1 bits (292), Expect = 4.200e-23
Identity = 52/81 (64.20%), Postives = 64/81 (79.01%), Query Frame = 0

Query:    2 APKQYTLDEVAGHNTEKDIWLIIGNEKTGGPKVYDVTKYLEEHPGGSEVLLDHAGKYADEMFEDIGHSGDAKEKLKTLLVG 83
            A K+Y LDE++ H T +  WLIIGN   GGPKVYDVTKYL++HPGG+EV+LD AG+ ADE FEDIGHS +A+ +LK  LVG
Sbjct:    3 AEKEYILDEISQHTTTESCWLIIGNASNGGPKVYDVTKYLDDHPGGAEVMLDVAGQDADEFFEDIGHSKEARAELKNYLVG 83

[Close]

BLAST of NO05G01060 vs. NCBI_GenBank
Match: EJK77858.1 (hypothetical protein THAOC_00279 [Thalassiosira oceanica])

HSP 1 Score: 115.9 bits (289), Expect = 9.500e-23
Identity = 50/81 (61.73%), Postives = 66/81 (81.48%), Query Frame = 0

Query:    4 KQYTLDEVAGHNTEKDIWLIIGNEKTGGPKVYDVTKYLEEHPGGSEVLLDHAGKYADEMFEDIGHSGDAKEKLKTLLVGEL 85
            K+YT+ EVA HNT +D WL+IG++ TGGPKVYDV+ YL++HPGG+EV+LD  G+ ADE FEDIGHS DA+++L   L+G L
Sbjct:    8 KEYTMAEVAEHNTAEDCWLVIGSDATGGPKVYDVSTYLDDHPGGAEVMLDVGGQDADEFFEDIGHSNDARDELAKHLIGTL 88

[Close]

BLAST of NO05G01060 vs. NCBI_GenBank
Match: XP_009037363.1 (hypothetical protein AURANDRAFT_16956, partial [Aureococcus anophagefferens] >EGB07999.1 hypothetical protein AURANDRAFT_16956, partial [Aureococcus anophagefferens])

HSP 1 Score: 114.4 bits (285), Expect = 2.800e-22
Identity = 51/77 (66.23%), Postives = 65/77 (84.42%), Query Frame = 0

Query:    8 LDEVAGHNTEKDIWLIIGNEKTGGPKVYDVTKYLEEHPGGSEVLLDHAGKYADEMFEDIGHSGDAKEKLKTLLVGEL 85
            L +VA H+ E+D ++IIGN+  GGPKVYDVTKYLE+HPGGSEV+ + AGKYAD+MFEDIGHS +A+++LK  LVG L
Sbjct:    1 LPQVAAHDQEEDCYIIIGNKGNGGPKVYDVTKYLEDHPGGSEVICELAGKYADDMFEDIGHSNEARKQLKEFLVGPL 77

[Close]

BLAST of NO05G01060 vs. NCBI_GenBank
Match: ELU09965.1 (hypothetical protein CAPTEDRAFT_153099 [Capitella teleta])

HSP 1 Score: 110.5 bits (275), Expect = 4.000e-21
Identity = 56/120 (46.67%), Postives = 77/120 (64.17%), Query Frame = 0

Query:    1 MAPKQYTLDEVAGHNTEKDIWLIIGNEKTGGPKVYDVTKYLEEHPGGSEVLLDHAGKYADEMFEDIGHSGDAKEKLKTLLVGELTPEDVEKLASEKVSRASELPSKGGLNPFAVVVLLVA 121
            MA KQ T +E++ HNT +D+W+ + N      KVYD+TK++EEHPGG EVLL+ AG YA E FED+GHS DA+E +    VGEL   D EK +S + S  +   ++GG     +V L +A
Sbjct:    1 MAAKQITKEELSKHNTARDLWIAVHN------KVYDITKFIEEHPGGEEVLLEQAGNYATEQFEDVGHSTDARELIMKYEVGELVEADHEKASSMRTSPLAPDSAEGGSMMSWLVPLAIA 114

The following BLAST results are available for this feature:

BLAST of NO05G01060 vs. NCBI_GenBank

BLAST of NO05G01060 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes)
Total hits: 20


Match Name	E-value	Identity	Description
EWM28245.1	1.000e-64	96.15	cytochrome b5 [Nannochloropsis gaditana]	[more]
GAX27651.1	4.800e-27	51.15	hypothetical protein FisN_13Hh257 [Fistulifera sol...	[more]
GAX27191.1	1.800e-26	50.38	hypothetical protein FisN_13Lh257 [Fistulifera sol...	[more]
OEU12251.1	2.700e-25	58.59	cytochrome b5 [Fragilariopsis cylindrus CCMP1102]	[more]
CBJ26085.1	5.000e-24	47.69	microsomal cytochrome b5 [Ectocarpus siliculosus]	[more]
AAM27441.1	8.600e-24	65.43	microsomal cytochrome b5 [Phaeodactylum tricornutu...	[more]
XP_002184806.1	4.200e-23	64.20	predicted protein [Phaeodactylum tricornutum CCAP ...	[more]
EJK77858.1	9.500e-23	61.73	hypothetical protein THAOC_00279 [Thalassiosira oc...	[more]
XP_009037363.1	2.800e-22	66.23	hypothetical protein AURANDRAFT_16956, partial [Au...	[more]
ELU09965.1	4.000e-21	46.67	hypothetical protein CAPTEDRAFT_153099 [Capitella ...	[more]

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Relationships

This gene is member of the following syntenic_region feature(s):


Feature Name	Unique Name	Species	Type
nonsL087	nonsL087	Nannochloropsis oceanica (N. oceanica IMET1)	syntenic_region
nonsL086	nonsL086	Nannochloropsis oceanica (N. oceanica IMET1)	syntenic_region
ncniR092	ncniR092	Nannochloropsis oceanica (N. oceanica IMET1)	syntenic_region
ngnoR118	ngnoR118	Nannochloropsis oceanica (N. oceanica IMET1)	syntenic_region

This gene is orthologous to the following gene feature(s):


Feature Name	Unique Name	Species	Type
NSK003762	NSK003762	Nannochloropsis salina (N. salina CCMP1776)	gene

The following polypeptide feature(s) derives from this gene:


Feature Name	Unique Name	Species	Type
NO05G01060.1	NO05G01060.1-protein	Nannochloropsis oceanica (N. oceanica IMET1)	polypeptide

The following gene feature(s) are orthologous to this gene:


Feature Name	Unique Name	Species	Type
jgi.p\|Nanoce1779_2\|669743	gene_2897	Nannochloropsis oceanica (N. oceanica CCMP1779)	gene
Naga_100004g31	gene2292	Nannochloropsis gaditana (N. gaditana B-31)	gene

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Species	Type
NO05G01060.1	NO05G01060.1	Nannochloropsis oceanica (N. oceanica IMET1)	mRNA

Sequences

The following sequences are available for this feature:

gene sequence
protein sequence

gene sequence

>NO05G01060 ID=NO05G01060|Name=NO05G01060|organism=Nannochloropsis oceanica|type=gene|length=2848bp
GTAACTTATCAATTTTATAGACACAATAAGCAAGGCGAGGTGTTCCTTGT
TTGGATTCGTGCACCCTTTTTGTCCGCATACGTGGCTTACTGGTCACTCT
CTGTCATCATCGGCTTCATCATTGCTTGCCCCCCTCTCGACACGGGAGCG
CGACCCTCTTCATCAATCCAATCCCCACCTGTTACTCTGGTCTTACAATC
TCTCTCCATACACCCCTTCATCTCAAACAGATAACTTCACCCGCACGCGC
CACTCAGTAGCGTCATGGCCCCCAAGCAGTACACATTGGACGAGGTTGCG
GGCCACAATACAGAGAAGGACATCTGGCTCATCATCGGAAACGAGAAGAC
AGgtgcgtaattttactttttattcgacagacaggtttgtcatgaacgat
gtacgtcctggaaataccccttttacgtactcctcaatgctaccctcctg
gcttccggaagcatccatcgaattgatcgcttcccccacccccttccccc
cctccctccccccatacgaacatagGCGGCCCCAAGGTGTACGATGTAAC
CAAGTACCTAGAAGAACACCCGGGAGGGTCAGAGGTTCTGCTTGACCACG
CCGGGAAGTACGCAGATGAGATGTTTGAGGACATTGGGCACTCGGGCGAT
GCCAAGGAAAAGCTCAAGACGCTGTTGGTGGGCGAGTTGACGgtaagtta
gggaaagaagcgttggagataggaaaacagtatgttgcaatttgtttcca
tgattgtttttgttcatgcagacatgagcaaccgctagttcaactttccg
ccctctctccctgccctccacccccttacacacccagCCCGAGGACGTGG
AGAAGTTAGCCAGCGAGAAAGTGTCGCGGGCGTCCGAGTTGCCATCCAAG
GGGGGTTTGAATCCCTTCGCCGTGGTGGTACTACTAGTAGCAATTGTCCT
GGGTCTGTACTTGTCGAAGCAATACAAGCAGTAGAGAGGAGACGCTGGAG
GGCAGGAGAGGGAGGGCTTGCTTGTTTCATTTTTGATGGTTTGACAGGGA
GAAGGAAATGAGATGGTGGTAGAGATGAACATGCGTGCATGAAGGAGAAG
GAATTGAAGGAGGGAGGGAGAGAGGGAGGAAGGGAGGGAGGGAGTAGCGG
GAGGAGGATATGTCGGAGAGAGAGTGTGGGTGAGTGTTTGCATGTGTGTG
TATGTGACGGGCGATGAAGGTCGCACAAGCAGCTGAAGAAAATGCAATAG
AAGAGGGGGGAAAGGAAAAGGCATCAGACGGTAGTATTTGGTATGAAAAA
GAAAGAGTAACAACAACACAGGACAAAACATAACTGAGGGTACCTGCGGA
ATAAGCGTCCGCCATTACCATGATGCCATGCCCTCCACCTCCAAAAAGGG
AATATTAGGGTTGATTGACAGGACGTAAAATACGGGATATACAAGCTGTA
GCCATGGATTGACGGCCTTCTCGAAGGAGAAAAGGAAGGCACGCTAACCT
CACATTCTTTCTCCCCTTTCACATACCGTCCGTCGTTCTTCTTGCATTTC
CTCGTCCTTCTCCCACCATAGCCCTCCCTCGCTAGCTATCCTTCCGAAGT
TGCAATGTCACAAACTTGCCGGCTTATCGCAGCTGCAACTGCTGCTGCTG
CCGCCGGCTATTGTGGTGGTGGAGGAGGGGACATTCCTTCCCACCTTTGC
TGTCAGCAAATCAAGCATGAACCAAGGCGGTGGTAGGTGAGCCACCCTTG
CGAGCGATGAACACCAAAACGAACCACACACAAAGTACAGGCTGGCCGTA
TGTATTTCTGCACTCACTTCAAATTTCACGACAAACATTTACCGACACTC
CATCCGTCCCCCCGTCCCTCCCTCCCGCTCTTGTACATGCGGTCACATCT
CTCTGTTTTCTTCCGGTCGATTCTTTTTATTCTTTGAGTTATTTTTTGTG
TAGACTTTGTACTCGAGGTTTCTGTATCCTGGTCGTCCTCCTCCTTGTCA
ATTTCATCCTTTTCTAAGTCGCGTTTCTTCAGGTATTTACCCTTCTTCTT
CTACTATCATCTGCTTCCACCATGTCACACATTAACAACGCTATCCATCC
TTTCCTCCCTCCCTCCCTCCTTCAATGTGCATCCTGAAATAGCTTCAAAA
AGAAGACGAAAGGCAAACGATATAAAAGATAGATTACAAGGCACTTATCG
CCTCTTCCCCTATCTCCCTTTCTCCACTTCTCCCTCCCTCCGCCCCTCAC
AACCAAGACCAAACCGAATTATATATGAAACCATCCCTTCCTCCTTCCCT
ACACACATGCCGTGCCCCGCGGCATAAGATAATAATCAAACGGTGGCGGA
TCCGGCAAAAACACACTCACCGCTCCCCCTCCTCCTCCTGCCCCATCCCC
CATCCCAGCCGCTGCAACAATATAGTTCTTACCTCCTCCTTTCCTCTCTC
CTGCTCTACTCCCGGCACGTAGGGCACTCGAAGTCATAAGGGATAGCCCC
CCTGTAGCTGCTGCTGGTGCTGGCGCTCCTGCTGCTGCTAGAGGGTGGTG
TGGATAATCCAACCTAAACGACTTCAACATTTGAGACGCTAGCCCAACCA
AGGCATCTTCTTCTACTCCTGTCAGAGAAGCGAGCTCCGGCCTCCAAGTC
GGAGAGACGCCTAGGGCGAGCCGTGCCAGGTACAGAAGGAGAGCAGCAAC
AAAGGAGGGAGGGTAGGGAGCTAGTGAGGTGAACTCTTGGACGATCGCAT
ACGAGGCAAAAAAGTGCGCGTGTTTTCGAAGTTGGGCTGGGGCGATCTGC
TGCTGCTGCTGTTGTTGTTGCTTTTGGGCAATGTCTTCCTTGGTCAGC

protein sequence of NO05G01060.1

>NO05G01060.1-protein ID=NO05G01060.1-protein|Name=NO05G01060.1|organism=Nannochloropsis oceanica|type=polypeptide|length=134bp
MAPKQYTLDEVAGHNTEKDIWLIIGNEKTGGPKVYDVTKYLEEHPGGSEV
LLDHAGKYADEMFEDIGHSGDAKEKLKTLLVGELTPEDVEKLASEKVSRA
SELPSKGGLNPFAVVVLLVAIVLGLYLSKQYKQ*

Synonyms

Publications