NO04G03300, NO04G03300 (gene) Nannochloropsis oceanica

BLAST of NO04G03300 vs. NCBI_GenBank
Match: EWM28746.1 (dna topoisomerase 6 subunit b [Nannochloropsis gaditana])

HSP 1 Score: 881.3 bits (2276), Expect = 1.600e-252
Identity = 445/550 (80.91%), Postives = 481/550 (87.45%), Query Frame = 0

Query:   26 MAPKNKAEVVESKTPAEFFSEHQSICGFDNPGRSLYTTVRELVENALDAAESIEVLPDVSITIEELTEEELNELRGMESRERVDVGLFHKASXXXXXXXXXXXXXXXTDK--HDNDLKPDEKQLQVSAPVALPPKKKERGT-SYYRVTCEDNGCGMAHDRIPDMLGRVLSSSKYGVRQTRGKFGLGSKMALIWSKKSTGLPIEVRSAHSTETSRAPGYVSRCILDIDIYRNEPAVKLHEKVANPQQWRGTRIDVVVEGNWSTYKTHIMTYFQQLAIITPYAKMDFTFRALSNAAREVVTQYARRSEQMPSPAQEVKHHPSSVNHLIIQQLLKRTKAKSLLVFLSAELACVNRPLALRIVKELGSGFTEDMGPDKMDANKTIRLTSMLKSVSIFKPPDGSCLSPAGEYNLRLGIMKELHPDYVATVSEQAAAFEGHPFIVEAGVSLGGQAVKDGINVYRFANRIPLLFEAGNDVVTQVANRRIHWQSYKIDPKRDRVGVFVSIVSTKIPFKGTGKEYIGDDTEEIRKCVKNALQRCCKQLKQHLLTRNAAR 573
            MA KN+ EVVESKTPAEFFSEHQSICGFDNPGRSLYTTVRELVENALDAAESI+ LP VS+ IEE+TE+ELNELRGMESRERVD+GLFHKA+                 K  H + L   EKQ+  ++      KK +RG  +YYRVTCEDNGCGMAH++IPDMLGRVLSSSKYGVRQTRGKFGLGSKMALIWSKKSTGLPIEVRSAHS E +    Y+S C+LDIDIYRN+P VKLHEKVAN + WRGTRIDVVVEGNWSTYKTHIMTYFQQLAIITPYA M+FTFR LSNA+REV+TQYARRSE MP PAQEVKHHPSS+NHLIIQQL+KRT  K+ L FL  ELACV RPLA RIVKELG+GF E+M P+ +DANKTIRLTSMLKS+SIFK PDGSCLSPAGEYNLRLGIMKELHPDYVATVSEQAAAFEGHPF+VEAGVSLGG A+KDGINVYRFANRIPLLFEAGNDVVTQVANRRI+WQSYKIDPKRDR+GVFVSIVSTKIPFKGTGKEYIGDDTE IRK VK+ALQRCCKQLKQHLLTRNAAR
Sbjct:    1 MARKNRIEVVESKTPAEFFSEHQSICGFDNPGRSLYTTVRELVENALDAAESIQELPYVSVVIEEITEDELNELRGMESRERVDLGLFHKATGKRRSGGEIQEVLAENMKELHSDAL---EKQMGQNSAALSNKKKGDRGARNYYRVTCEDNGCGMAHEKIPDMLGRVLSSSKYGVRQTRGKFGLGSKMALIWSKKSTGLPIEVRSAHSLEENHPAAYISHCVLDIDIYRNQPFVKLHEKVANIKGWRGTRIDVVVEGNWSTYKTHIMTYFQQLAIITPYAHMEFTFRTLSNASREVITQYARRSEHMPPPAQEVKHHPSSINHLIIQQLIKRTSTKTFLNFLCTELACVTRPLAARIVKELGNGFAENMVPEDIDANKTIRLTSMLKSISIFKLPDGSCLSPAGEYNLRLGIMKELHPDYVATVSEQAAAFEGHPFVVEAGVSLGGNALKDGINVYRFANRIPLLFEAGNDVVTQVANRRINWQSYKIDPKRDRIGVFVSIVSTKIPFKGTGKEYIGDDTEAIRKSVKSALQRCCKQLKQHLLTRNAAR 547          

BLAST of NO04G03300 vs. NCBI_GenBank
Match: OEU16885.1 (topo-VIb_trans-domain-containing protein [Fragilariopsis cylindrus CCMP1102])

HSP 1 Score: 574.3 bits (1479), Expect = 4.200e-160
Identity = 296/551 (53.72%), Postives = 394/551 (71.51%), Query Frame = 0

Query:   29 KNKAEVVESKTPAEFFSEHQSICGFDNPGRSLYTTVRELVENALDAAESIEVLPDVSITIEELTEEELNELRGMESRERVDVGLF-------HKASXXXXXXXXXXXXXXXTDKHDNDLKPDEKQLQVSAPVALPPKKKERGTSYYRVTCEDNGCGMAHDRIPDMLGRVLSSSKYGVRQTRGKFGLGSKMALIWSKKSTGLPIEVRSAHSTETSRAPGYVSRCILDIDIYRNEPAVKLHEKVANPQQWRGTRIDVVVEGNWSTYKTHIMTYFQQLAIITPYAKMDFTFRALSNAAREVVTQYARRSEQMPSPAQEVKHHPSSVNHLIIQQLLKRTKAKSLLVFLSAELACVNRPLALRIVKELGSGFTEDMGPDKMDANKTIRLTSMLKSVSIFKPPDGSCLSPAGEYNLRLGIMKELHPDYVATVSEQAAAFEGHPFIVEAGVSLGGQAVKDGINVYRFANRIPLLFEAGNDVVTQVANRRIHWQSYKIDPKRDRVGVFVSIVSTKIPFKGTGKEYIGDDTEEIRKCVKNALQRCCKQLKQHLLTRNAAR 573
            + + E+   K+PAEFF+++Q+I GFDN G+SLYT++RELVEN+LDA ESI VLP + + IEEL ++E N++RG+    + DV LF       + ++  XXXXXXXXX    +  +D D KP +            P+KK     Y+++  +DNGCGM H++IPD+LGRVLS SKYGVRQTRGKFGLG+KMALIWSKKSTG+PI++ +AHS         VS C+LDI+I  N P +  H K  N  +W GT +++++ GNW+TY++ I++YFQQLAIITPY +++  +   S   R +  ++ RRSEQMP   +E+KHHPSSVN++I+ QL++R+  K+++ FL+ EL+CV RPLA RIV E G    + + P  M + +  +L  +L++V +FK PDGSCLSP GEYNL LGI K L P++VAT  ++ +A+EGHPF+VEA VSLGG+  KDG+NV RFANRIPLLFE G DV T+VAN +I W  YKID KRDR+GVFVSIVSTKIP+KGTGKEYIGDD  EI   VK A+Q CC+QL+ HLL RNA R
Sbjct:   12 EGRGEIQRQKSPAEFFADNQAIAGFDNYGKSLYTSIRELVENSLDACESIGVLPTIQVYIEELNQDEFNKIRGISVATK-DVELFQQKGGKGNSSAGAXXXXXXXXXGVNGSGGNDKDSKPTK-----------TPRKKASQEGYFQLKVKDNGCGMNHEKIPDLLGRVLSGSKYGVRQTRGKFGLGAKMALIWSKKSTGVPIKITTAHS---------VSICVLDINITNNCPNIIQHTKSKNTTEWVGTELELLIAGNWTTYRSRIVSYFQQLAIITPYCQLELVYENRSEPQRNMTLRHDRRSEQMPPLPREIKHHPSSVNNIIVSQLIERSNCKTIIDFLTKELSCVTRPLAQRIVTECGWDEDDIVNPSDMTSRQLTKLVQVLRTVKMFKAPDGSCLSPLGEYNLNLGIRKVLEPEFVATARDKPSAYEGHPFLVEAAVSLGGKEAKDGLNVVRFANRIPLLFEGGADVCTRVANNKIKWSLYKIDQKRDRIGVFVSIVSTKIPYKGTGKEYIGDDITEISVSVKKAIQSCCQQLRVHLLKRNALR 541          

BLAST of NO04G03300 vs. NCBI_GenBank
Match: XP_002182945.1 (type II DNA topoisomerase 6 subunit [Phaeodactylum tricornutum CCAP 1055/1] >EEC45681.1 type II DNA topoisomerase 6 subunit [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 571.6 bits (1472), Expect = 2.700e-159
Identity = 290/546 (53.11%), Postives = 382/546 (69.96%), Query Frame = 0

Query:   33 EVVESKTPAEFFSEHQSICGFDNPGRSLYTTVRELVENALDAAESIEVLPDVSITIEELTEEELNELRGMES--RERVDVGLFHKASXXXXXXXXXXXXXXXTDKHDNDLKPDEKQLQVSAPVAL----PPKKKERGTSYYRVTCEDNGCGMAHDRIPDMLGRVLSSSKYGVRQTRGKFGLGSKMALIWSKKSTGLPIEVRSAHSTETSRAPGYVSRCILDIDIYRNEPAVKLHEKVANPQQWRGTRIDVVVEGNWSTYKTHIMTYFQQLAIITPYAKMDFTFRALSNAAREVVTQYARRSEQMPSPAQEVKHHPSSVNHLIIQQLLKRTKAKSLLVFLSAELACVNRPLALRIVKELGSGFTEDMGPDKMDANKTIRLTSMLKSVSIFKPPDGSCLSPAGEYNLRLGIMKELHPDYVATVSEQAAAFEGHPFIVEAGVSLGGQAVKDGINVYRFANRIPLLFEAGNDVVTQVANRRIHWQSYKIDPKRDRVGVFVSIVSTKIPFKGTGKEYIGDDTEEIRKCVKNALQRCCKQLKQHLLTRNAAR 573
            EV   K+PAEFF+E+Q+I GFDN G+SLYT++RELVEN+LDA ESI  LP++SI I+E T+EE N L  M++  R++ D+ LF                    +    D KP ++   V     +      +KK    +Y+++T +DNGCGM+H  IP++LGRVLS SKYGVRQTRGKFGLG+KMALIW+KKSTG PI + ++H  +   AP   S C+LDIDIY+N P +  H    N   W GT + V++ GNW+TYK+ I+ Y QQLAIITPYA ++  +  +S++ R +  +Y RRS+QMP PA+ +KHHP+SVN+L+IQQL++ TK ++L  FLS EL+ V+ PLA R+V EL  GF E M P  ++  +  RL  +L+ V +FK PDGSCLSP GEYNL LGI K L PD +AT  ++  A+EGHPF+VEA VSLGG+ VK+GI V RFANRIPLLFE G DV T+VAN +I W +YK+D KRDR+GVFVSIVSTK+PFKGT KEYIGDD  EI++ VK ALQ CC+QL+ +L  R+A +
Sbjct:   15 EVQVQKSPAEFFAENQAIAGFDNLGKSLYTSLRELVENSLDACESIHELPEISIEIKEYTQEEFNALDVMKTSPRKKRDMQLF--------------------ETKKKDKKPPKEATDVDEITTVENSGKKRKKAPQDAYFKLTVKDNGCGMSHSAIPNLLGRVLSGSKYGVRQTRGKFGLGAKMALIWAKKSTGQPIRIMTSHRPDGGVAPERASACVLDIDIYKNAPRILEHTTRKNTDGWMGTEMSVLIAGNWTTYKSRIVQYLQQLAIITPYAVLELAYINISDSKRNLHVRYDRRSDQMPPPAKTIKHHPASVNNLVIQQLIQFTKTRTLFKFLSTELSTVSPPLARRLVTEL--GFDESMPPSSLEDKEITRLVQLLRQVQLFKAPDGSCLSPLGEYNLNLGIRKVLEPDLIATSRDKPGAYEGHPFLVEAAVSLGGKEVKEGITVIRFANRIPLLFEGGADVATRVANTKIRWSNYKMDYKRDRIGVFVSIVSTKVPFKGTSKEYIGDDATEIQQSVKRALQSCCQQLRGYLAKRSALK 538          

BLAST of NO04G03300 vs. NCBI_GenBank
Match: XP_002289341.1 (topoisomerase [Thalassiosira pseudonana CCMP1335] >EED92878.1 topoisomerase [Thalassiosira pseudonana CCMP1335])

HSP 1 Score: 570.1 bits (1468), Expect = 7.800e-159
Identity = 289/552 (52.36%), Postives = 379/552 (68.66%), Query Frame = 0

Query:   26 MAPK----NKAEVVESKTPAEFFSEHQSICGFDNPGRSLYTTVRELVENALDAAESIEVLPDVSITIEELTEEELNELRGMESRERVDVGLFHKASXXXXXXXXXXXXXXXTDKHDNDLKPDEKQLQVSAPVALPPKKKERGTSYYRVTCEDNGCGMAHDRIPDMLGRVLSSSKYGVRQTRGKFGLGSKMALIWSKKSTGLPIEVRSAHSTETSRAPGYVSRCILDIDIYRNEPAVKLHEKVANPQQWRGTRIDVVVEGNWSTYKTHIMTYFQQLAIITPYAKMDFTFRALSNAAREVVTQYARRSEQMPSPAQEVKHHPSSVNHLIIQQLLKRTKAKSLLVFLSAELACVNRPLALRIVKELGSGFTEDMGPDKMDANKTIRLTSMLKSV-SIFKPPDGSCLSPAGEYNLRLGIMKELHPDYVATVSEQAAAFEGHPFIVEAGVSLGGQAVKDGINVYRFANRIPLLFEAGNDVVTQVANRRIHWQSYKIDPKRDRVGVFVSIVSTKIPFKGTGKEYIGDDTEEIRKCVKNALQRCCKQLKQHLLTRNAAR 573
            M PK     K E+ +SK+PAEFF+E+QSI GFDNPG+SLYTT+RELVEN+LDA ES++VLPD+S+ IEE+T+++ NE+RG+ +                             D                         +  G +Y+ VT  DNGCGMAHD IPD+LGRVLS SKYGVRQTRGKFGLG+KMALIWSKKSTG+PI ++++H            + I  IDIY+N P +  H++  N + W GT + V+V GNW+TYK+ ++ Y QQLAIITPYA+++ ++   S+  + +  ++ RRSEQMPS A+EVKHHPSSVN+L++QQLL+ +K+K+LL FL+ +L+ ++  +A R+++ LG  F EDM P +MD  +  RL  +L+S   +FK PDG CLSP GEYNL LGI K + P+ +AT  ++  A++GHPFIVEA VSLGG+  K+GI V RFANRIPLLFE G DV T+VAN +I W SYKID KRD++GVFVSIVSTK+PFKGTGKEYIGDD  EI+  VK ALQ CC+QL+ HL  RNA R
Sbjct:    1 MPPKKSSSKKDEIQQSKSPAEFFAENQSIAGFDNPGKSLYTTLRELVENSLDACESVDVLPDISVVIEEMTQDQFNEMRGVPT--------------SGGPGKKKKGVDGSDDAAVXXXXXXXXXXXXXXXXXXXXXXRAGGDAYFLVTVRDNGCGMAHDAIPDLLGRVLSGSKYGVRQTRGKFGLGAKMALIWSKKSTGVPIRIKTSH-----------VKGIYIIDIYKNCPRIIEHKQKDNTENWIGTEMQVLVSGNWTTYKSRVVQYLQQLAIITPYARLEMSYSNRSDEKKGMNLRFERRSEQMPSQAREVKHHPSSVNNLLVQQLLENSKSKTLLKFLTGDLSGISPSVAKRLIERLGDAFEEDMSPAEMDDKQITRLVQLLRSTDDLFKSPDGGCLSPLGEYNLNLGIQKVVEPEIIATARDKPGAYDGHPFIVEAAVSLGGKEAKEGITVVRFANRIPLLFEGGADVATRVANSKIKWSSYKIDHKRDKIGVFVSIVSTKVPFKGTGKEYIGDDITEIQLSVKRALQNCCQQLRVHLAKRNALR 527          

BLAST of NO04G03300 vs. NCBI_GenBank
Match: XP_020888533.1 (DNA topoisomerase 6 subunit B [Arabidopsis lyrata subsp. lyrata])

HSP 1 Score: 560.5 bits (1443), Expect = 6.200e-156
Identity = 292/548 (53.28%), Postives = 379/548 (69.16%), Query Frame = 0

Query:   28 PKNKAE-VVESKTPAEFFSEHQSICGFDNPGRSLYTTVRELVENALDAAESIEVLPDVSITIEELTEEELNELRGMESRERVDVGLFHKASXXXXXXXXXXXXXXXTDKHDNDLKPDEKQLQVSAPVALPPKKKERG-TSYYRVTCEDNGCGMAHDRIPDMLGRVLSSSKYGVRQTRGKFGLGSKMALIWSKKSTGLPIEVRSAHSTETSRAPGYVSRCILDIDIYRNEPAVKLHEKVANPQQWRGTRIDVVVEGNWSTYKTHIMTYFQQLAIITPYAKMDFTFRALSNAAREVVT-QYARRSEQMPSPAQEVKHHPSSVNHLIIQQLLKRTKAKSLLVFLSAELACVNRPLALRIVKELGSGFTEDMGPDKMDANKTIRLTSMLKSVSIFKPPDGSCLSPAGEYNLRLGIMKELHPDYVATVSEQAAAFEGHPFIVEAGVSLGGQAVKDGINVYRFANRIPLLFEAGNDVVTQVANRRIHWQSYKIDPKRDRVGVFVSIVSTKIPFKGTGKEYIGDDTEEIRKCVKNALQRCCKQLKQHLLTRNAAR 573
            PKN  E  ++ K+PAEFF+E+++I GFDNPG+SLYTTVRELVENALD+AESI  LP+V +TIEE+ + + N + G+  RERVD  L+                   ++    +L   +K  +      L    K RG  SYY+VTC+DNG GM HD IP+M GRVLS +KYG++QTRGKFGLG+KMALIWSK STGLPIE+ S     + ++  YV+ C LDIDI+RN P + LHEK  N ++W G  I VV+EGNW+TY++ I+ Y +Q+A+ITPYA+  F FR +S    + VT ++ RR++ MP    E KHHPSSV+ L+I++L+  T  K+LL FL  E   +N+ LA R++ E+G  F   M    + + + +R+  + +    F  P G CLSPAGEYNLRLGI+KELHPD VAT S  A  FEGHPFIVEAGVSLGG+ VK GIN++RFANRIPLLFE G DVVT+ A +RI+W SYKI+  +D++GVFVSIVSTKIPFKGTGKEYIGDD  EI   VK+A+Q+CC QLK  ++ R  AR
Sbjct:   17 PKNSNESKLKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENALDSAESISELPEVEVTIEEIVKSKFNSMIGLIDRERVDTQLYDDYETEKAREKRLAKEARASEIQAKNLASGKKNKEPGVSKVL----KARGEASYYKVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISS-----SMKSQNYVTFCRLDIDIHRNIPHIHLHEKKGNKEKWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQ--FLFRFISETPEKNVTIKFTRRTDVMPPIPIETKHHPSSVDLLLIKRLITDTSKKTLLQFLQNEFVNINKTLATRLIGEMGPDFGPSMAVKSVTSQQMVRIHQLFRQAK-FDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSLGGRDVKQGINIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIATAVKSAIQQCCIQLKSKIVKRLQAR 552          

BLAST of NO04G03300 vs. NCBI_GenBank
Match: XP_006297133.1 (DNA topoisomerase 6 subunit B [Capsella rubella] >EOA30031.1 hypothetical protein CARUB_v10013135mg [Capsella rubella])

HSP 1 Score: 560.1 bits (1442), Expect = 8.100e-156
Identity = 287/540 (53.15%), Postives = 377/540 (69.81%), Query Frame = 0

Query:   35 VESKTPAEFFSEHQSICGFDNPGRSLYTTVRELVENALDAAESIEVLPDVSITIEELTEEELNELRGMESRERVDVGLFHKASXXXXXXXXXXXXXXXTDKHDNDLKPDEKQLQVSAPVALPPKKKERG-TSYYRVTCEDNGCGMAHDRIPDMLGRVLSSSKYGVRQTRGKFGLGSKMALIWSKKSTGLPIEVRSAHSTETSRAPGYVSRCILDIDIYRNEPAVKLHEKVANPQQWRGTRIDVVVEGNWSTYKTHIMTYFQQLAIITPYAKMDFTFRALSNAAREVVT-QYARRSEQMPSPAQEVKHHPSSVNHLIIQQLLKRTKAKSLLVFLSAELACVNRPLALRIVKELGSGFTEDMGPDKMDANKTIRLTSMLKSVSIFKPPDGSCLSPAGEYNLRLGIMKELHPDYVATVSEQAAAFEGHPFIVEAGVSLGGQAVKDGINVYRFANRIPLLFEAGNDVVTQVANRRIHWQSYKIDPKRDRVGVFVSIVSTKIPFKGTGKEYIGDDTEEIRKCVKNALQRCCKQLKQHLLTRNAAR 573
            ++ K+PAEFF+E+++I GFDNPG+SLYTTVRELVENALD+AESI  LP+V +TIEE+ + + N + G+  RERVD  L+                   ++    +L   +K  +      L    K RG  SYY+VTC+DNG GM HD IP+M GRVLS +KYG++QTRGKFGLG+KMALIWSK STGLPIE+ S     + ++  YV+ C LDIDI+RN P + LHEK  N ++W G  I VV+EGNW+TY++ I+ Y +Q+A+ITPYA+  F FR +S    + VT ++ RR++ MP    E KHHPSSV+ L+I++L+  T  K+LL FL  E   +N+ LA R++ E+G  F+  M    + + + +R+  + +    F  P G CLSPAGEYNLRLGI+KELHPD VAT S  A  FEGHPFIVEAGVS+GG+ VK GIN++RFANRIPLLFE G DVVT+ A++RI+W SYKI+  +D++GVFVSIVSTKIPFKGTGKEYIGDD  EI   VK+A+Q+CC QLK  ++ R  AR
Sbjct:   25 LKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENALDSAESISELPEVEVTIEEIVKSKFNSMIGLIDRERVDTQLYDDYETEKAREKRLAKEARASEIQAKNLASGKKNKEPGVSKVL----KARGEASYYKVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISS-----SMKSQNYVTFCRLDIDIHRNIPHIHLHEKKGNKEKWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQ--FLFRFISETPEKNVTIKFTRRTDVMPPIPVETKHHPSSVDLLLIKRLVTDTSKKTLLQFLQNEFVNINKTLATRLIGEMGPDFSPSMAVKSVTSQQMVRIHQLFRQAK-FDDPSGGCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSVGGRDVKQGINIFRFANRIPLLFEQGGDVVTRTASKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIATAVKSAIQQCCIQLKSKIVKRLQAR 552          

BLAST of NO04G03300 vs. NCBI_GenBank
Match: CBJ26118.1 (DNA topoisomerase VI subunit B-like protein Topoisomerase 6 subunit B [Ectocarpus siliculosus])

HSP 1 Score: 559.3 bits (1440), Expect = 1.400e-155
Identity = 292/558 (52.33%), Postives = 366/558 (65.59%), Query Frame = 0

Query:   29 KNKAEVVESKTPAEFFSEHQSICGFDNPGRSLYTTVRELVENALDAAESIEVLPDVSITIEELTEEELNELRGMESRERVDVGLFHKASXXXXXXXXXXXXXXXTDKHDNDL-KPDEKQLQVSAPVA-LPPKKKE---------RGTS---YYRVTCEDNGCGMAHDRIPDMLGRVLSSSKYGVRQTRGKFGLGSKMALIWSKKSTGLPIEVRSAHSTETSRAPGYVSRCILDIDIYRNEPAVKLHEKVANPQQWRGTRIDVVVEGNWSTYKTHIMTYFQQLAIITPYAKMDFTFRALSNAAREVVTQYARRSEQMPSPAQEVKHHPSSVNHLIIQQLLKRTKAKSLLVFLSAELACVNRPLALRIVKELGSGFTEDMGPDKMDANKTIRLTSMLKSVSIFKPPDGSCLSPAGEYNLRLGIMKELHPDYVATVSEQAAAFEGHPFIVEAGVSLGGQAVKDGINVYRFANRIPLLFEAGNDVVTQVANRRIHWQSYKIDPKRDRVGVFVSIVSTKIPFKGTGKEYIGDDTEEIRKCVKNALQRCCKQLKQHLLTRNAAR 573
            K   E V+SK+PAEFF+++Q+I GFDN G+SLYTT+RELVEN+LDAAESI  LP +S+TIEE T               VD GLF  +                    +     P E      A  A L PK+K+         +G S   YYRV C+DNG GM HD+IPDMLG VLSSSKYGVRQ RGK+G+G+KMALIWSKKSTGLPI+V S  S++    P ++++C+LDIDIY+N P+V  H K+ NP+ WRGT + V V GNW TYK+ ++ Y QQLAIITPYA+++  + + +N  +++   Y RRSEQMP  A EVKHHP SVN+L++QQL+ RTK K L+ FL  +L+ ++RPL+ RI+ ELG GF   M P  +      RLT ML+ V  FKPPDG+CLSPAGEYNLR                                       VK+G+NVYRFANRIPLLFEAG+DVVT+VA +RI+W SYK+D KRD++GVFVS VSTKIPFKGT KEYIGDDTEEI+K VK+ALQ+CC+QL+ HLL RNA R
Sbjct:    3 KGSGEAVKSKSPAEFFADNQNIAGFDNAGKSLYTTIRELVENSLDAAESIGALPHISLTIEEFTXXXXXXXXXXXXXXVVDAGLFKASGGKKSTSKATKALPKPAVAEEGSADDPQEPSAAAGATAADLDPKEKKAKNDKKGRSKGDSHSLYYRVKCKDNGVGMPHDKIPDMLGVVLSSSKYGVRQARGKYGMGAKMALIWSKKSTGLPIQVVSGCSSDVKDPPNFLTKCVLDIDIYKNRPSVHEHVKLPNPEGWRGTEMTVCVSGNWQTYKSRVLQYLQQLAIITPYAELELEYHSHANPKKDLTAVYRRRSEQMPPLAYEVKHHPGSVNNLLVQQLIHRTKCKDLVGFLMKDLSSIDRPLSERIIAELGEGFHPKMQPSALTVKMVSRLTQMLRDVKRFKPPDGACLSPAGEYNLR--------------------------------------QVKEGVNVYRFANRIPLLFEAGSDVVTRVAMKRINWSSYKVDHKRDKIGVFVSTVSTKIPFKGTSKEYIGDDTEEIKKAVKHALQQCCQQLRTHLLRRNALR 522          

BLAST of NO04G03300 vs. NCBI_GenBank
Match: XP_010509333.1 (PREDICTED: DNA topoisomerase 6 subunit B-like [Camelina sativa])

HSP 1 Score: 559.3 bits (1440), Expect = 1.400e-155
Identity = 286/540 (52.96%), Postives = 377/540 (69.81%), Query Frame = 0

Query:   35 VESKTPAEFFSEHQSICGFDNPGRSLYTTVRELVENALDAAESIEVLPDVSITIEELTEEELNELRGMESRERVDVGLFHKASXXXXXXXXXXXXXXXTDKHDNDLKPDEKQLQVSAPVALPPKKKERG-TSYYRVTCEDNGCGMAHDRIPDMLGRVLSSSKYGVRQTRGKFGLGSKMALIWSKKSTGLPIEVRSAHSTETSRAPGYVSRCILDIDIYRNEPAVKLHEKVANPQQWRGTRIDVVVEGNWSTYKTHIMTYFQQLAIITPYAKMDFTFRALSNA-AREVVTQYARRSEQMPSPAQEVKHHPSSVNHLIIQQLLKRTKAKSLLVFLSAELACVNRPLALRIVKELGSGFTEDMGPDKMDANKTIRLTSMLKSVSIFKPPDGSCLSPAGEYNLRLGIMKELHPDYVATVSEQAAAFEGHPFIVEAGVSLGGQAVKDGINVYRFANRIPLLFEAGNDVVTQVANRRIHWQSYKIDPKRDRVGVFVSIVSTKIPFKGTGKEYIGDDTEEIRKCVKNALQRCCKQLKQHLLTRNAAR 573
            ++ K+PAEFF+E+++I GFDNPG+SLYTTVRELVENALD+AESI  LP+V +TIEE+ + + N + G+  RERVD  L+                   ++    +L   +K  +      L    K RG  SYY+VTC+DNG GM HD IP+M GRVLS +KYG++QTRGKFGLG+KMALIWSK STGLPIE+ S     + ++  YV+ C LDIDI+RN P + LHEK  N ++W G  I VV+EGNW+TY++ I+ Y +Q+A+ITPYA+  F FR +S    + V+ ++ RR++ MP    E KHHPSSV+ L+I++L+  T  K+LL FL  E   +N+ LA R++ E+G  F+  M    + + + +R+  + +    F  P G+CLSPAGEYNLRLGI+KELHPD VAT S  A  FEGHPFIVEAGVS+GG+ VK GIN++RFANRIPLLFE G DVVT+ A +RI+W SYKI+  +D++GVFVSIVSTKIPFKGTGKEYIGDD  EI   VK+A+Q+CC QLK  ++ R  AR
Sbjct:   26 LKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENALDSAESISELPEVEVTIEEIVKSKFNSMIGLIDRERVDTQLYDDYETEKAREKRLAKEARVSEIQAKNLASGKKNKEPGVSKVL----KARGEASYYKVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISS-----SMKSQNYVTFCRLDIDIHRNIPHIHLHEKKGNKEKWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQ--FLFRFISETPEKNVIIKFTRRTDVMPPIPIETKHHPSSVDLLLIKRLITDTAKKTLLQFLQNEFVNINKTLATRLIGEMGPDFSPSMAVKSVTSQQMVRIHQLFRQAK-FDDPSGNCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSVGGRDVKQGINIFRFANRIPLLFEQGGDVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIATAVKSAIQQCCVQLKSKIVKRLQAR 553          

BLAST of NO04G03300 vs. NCBI_GenBank
Match: XP_010488064.1 (PREDICTED: DNA topoisomerase 6 subunit B [Camelina sativa])

HSP 1 Score: 558.9 bits (1439), Expect = 1.800e-155
Identity = 287/540 (53.15%), Postives = 377/540 (69.81%), Query Frame = 0

Query:   35 VESKTPAEFFSEHQSICGFDNPGRSLYTTVRELVENALDAAESIEVLPDVSITIEELTEEELNELRGMESRERVDVGLFHKASXXXXXXXXXXXXXXXTDKHDNDLKPDEKQLQVSAPVALPPKKKERG-TSYYRVTCEDNGCGMAHDRIPDMLGRVLSSSKYGVRQTRGKFGLGSKMALIWSKKSTGLPIEVRSAHSTETSRAPGYVSRCILDIDIYRNEPAVKLHEKVANPQQWRGTRIDVVVEGNWSTYKTHIMTYFQQLAIITPYAKMDFTFRALSNAAREVVT-QYARRSEQMPSPAQEVKHHPSSVNHLIIQQLLKRTKAKSLLVFLSAELACVNRPLALRIVKELGSGFTEDMGPDKMDANKTIRLTSMLKSVSIFKPPDGSCLSPAGEYNLRLGIMKELHPDYVATVSEQAAAFEGHPFIVEAGVSLGGQAVKDGINVYRFANRIPLLFEAGNDVVTQVANRRIHWQSYKIDPKRDRVGVFVSIVSTKIPFKGTGKEYIGDDTEEIRKCVKNALQRCCKQLKQHLLTRNAAR 573
            ++ K+PAEFF+E+++I GFDNPG+SLYTTVRELVENALD+AESI  LP+V +TIEE+ + + N + G+  RERVD  L+                   ++    +L   +K  +      L    K RG  SYY+VTC+DNG GM HD IP+M GRVLS +KYG++QTRGKFGLG+KMALIWSK STGLPIE+ S     + ++  YV+ C LDIDI+RN P + LHEK  N ++W G  I VV+EGNW+TY++ I+ Y +Q+A+ITPYA+  F FR +S    + VT ++ RR++ MP    E KHHPSSV+ L+I++L+  T  K+LL FL  E   +N+ LA R++ E+G  F+  M    + + + +R+  + +    F  P G+CLSPAGEYNLRLGI+KELHPD VAT S  A  FEGHPFIVEAGVS+GG+ VK GIN++RFANRIPLLFE G DVVT+ A +RI+W SYKI+  +D++GVFVSIVSTKIPFKGTGKEYIGDD  EI   VK+A+Q+CC QLK  ++ R  AR
Sbjct:   26 LKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENALDSAESISELPEVEVTIEEIVKSKFNSMIGLIDRERVDTQLYDDYETEKAREKRLAKEARVSEIQAKNLASGKKNKEPGVSKVL----KARGEASYYKVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISS-----SMKSQNYVTFCRLDIDIHRNIPHIHLHEKKGNKEKWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQ--FLFRFISETPEKNVTIKFTRRTDVMPPIPIETKHHPSSVDLLLIKRLITDTAKKTLLQFLQNEFVNINKTLATRLIGEMGPDFSPSMAVKSVTSQQMVRIHQLFRQAK-FDDPSGNCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSVGGRDVKQGINIFRFANRIPLLFEQGGDVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIATAVKSAIQQCCVQLKSKIVKRLQAR 553          

BLAST of NO04G03300 vs. NCBI_GenBank
Match: XP_010544238.1 (PREDICTED: DNA topoisomerase 6 subunit B isoform X1 [Tarenaya hassleriana])

HSP 1 Score: 558.9 bits (1439), Expect = 1.800e-155
Identity = 286/544 (52.57%), Postives = 376/544 (69.12%), Query Frame = 0

Query:   30 NKAEVVESKTPAEFFSEHQSICGFDNPGRSLYTTVRELVENALDAAESIEVLPDVSITIEELTEEELNELRGMESRERVDVGLFHKASXXXXXXXXXXXXXXXTDKHDNDLKPDEKQLQVSAPVALPPKKKERGTSYYRVTCEDNGCGMAHDRIPDMLGRVLSSSKYGVRQTRGKFGLGSKMALIWSKKSTGLPIEVRSAHSTETSRAPGYVSRCILDIDIYRNEPAVKLHEKVANPQQWRGTRIDVVVEGNWSTYKTHIMTYFQQLAIITPYAKMDFTFRALSNAAREVVT-QYARRSEQMPSPAQEVKHHPSSVNHLIIQQLLKRTKAKSLLVFLSAELACVNRPLALRIVKELGSGFTEDMGPDKMDANKTIRLTSMLKSVSIFKPPDGSCLSPAGEYNLRLGIMKELHPDYVATVSEQAAAFEGHPFIVEAGVSLGGQAVKDGINVYRFANRIPLLFEAGNDVVTQVANRRIHWQSYKIDPKRDRVGVFVSIVSTKIPFKGTGKEYIGDDTEEIRKCVKNALQRCCKQLKQHLLTRNAAR 573
            +K   ++ K+PAEFF+E+++I GFDNPG+SLYTTVRELVENALD+AESI  LP+V ITIEE+ + + N + G+  RERVD  L+                    +    +    +K   V  P      K     SYY+VTC+DNG GM HD IP+M GRVLS +KYG++QTRGKFGLG+KMALIWSK STGLPIE+ S     + +   YV+ C LDIDI+RN P V LHEK  N ++W G  I VV+EGNW+TY++ I+ Y +Q+A+ITPYA+  F FR +S+   + +T ++ARR++ MP    E KHHPSSV+ L+I++L+  T  K+LL FL  E   +N+ LA R++ E+G  F+ +M    + + + +R+  + +    F  P G CLSPAGEYNLRLG++KELHPD VAT S  A  FEGHPFIVEAGVSLGG+ VK GIN++RFANRIPLLFE G DVVT+ A +RI+W +YKI+  +D++GVF+SIVSTKIPFKGTGKEYIGDD  EI   VK+A+Q+CC QLK  ++ R  AR
Sbjct:   21 SKDSNLKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENALDSAESISQLPEVEITIEEIGKSKFNSMIGLVDRERVDEKLYDDYETVKAREKRLAKEARAQEIQAKNAASGKK---VKEPAVSKVLKGRGEASYYKVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISS-----SMKGQSYVTFCRLDIDIHRNIPHVHLHEKKGNTERWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQ--FLFRFISDTPEKNITIKFARRTDVMPPVPVETKHHPSSVDLLLIKRLIADTSKKTLLQFLQNEFVNINKSLAARLIGEMGPDFSPNMAVKSLTSQQMVRIHQLFRQAK-FDDPSGDCLSPAGEYNLRLGVIKELHPDMVATYSGSAQVFEGHPFIVEAGVSLGGKDVKQGINIFRFANRIPLLFEQGGDVVTRTALKRINWSTYKINQTQDKIGVFISIVSTKIPFKGTGKEYIGDDISEIATAVKSAIQQCCNQLKSKIVKRIQAR 553