NO04G01130, NO04G01130 (gene) Nannochloropsis oceanica

BLAST of NO04G01130 vs. NCBI_GenBank
Match: XP_024376935.1 (probable E3 ubiquitin-protein ligase HERC4 isoform X1 [Physcomitrella patens] >PNR54602.1 hypothetical protein PHYPA_008279 [Physcomitrella patens])

HSP 1 Score: 419.1 bits (1076), Expect = 5.500e-113
Identity = 339/1132 (29.95%), Postives = 505/1132 (44.61%), Query Frame = 0

Query:  291 PTPPLLKPTKSKYGKKNVANLCSTLYHTVAVTTTGEVYGCGVNEDGQVSPDKEDQYLWAPVLVERLLSQRVIQVSCGENHTACLTSSGAVLSFGNNEVGQLGHSAGATTTGEIVRSSRGSRRVEPRTVVGLGGKLIKQIACGYLFTVMLSSEGEVFSCGVGGCVGGQDGKDRFQAERVDALMFVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLPPCPSVSPVPGEEELGGGGTVSPYRAPVLVSQLNGLEVCMLACGDAHTLALTKSGQVWSMGANSSGQLGTGASATNAEPSIHPSSSSWRATEVQGLQTAHVFMVAAGGEQSFAXXXXXXXXXXXXXXXXXXKTIIGTAVPTTAATPGLSLDESTLDLIRKFSVIKPATSFLTTAQLLTLIQQ-AASTHQPDFLERTLFDIFSSPSLLNGSFL-SVAHSSSLTAKSSTXRSCSTSTLS---GLDVQGLESIYVAVMKLNHTPLLSRLMHA----FSTALDHLLPVAKSLYDPDSLRCLLAYWQCPLNSNTSLSRDTVLKLCEAIVSLPGDGRVQLFAWARADYPPHLFATRLLRPLHSHLEYHLKVNFGKGRAVPMLAVIMNYLYAANERIREDGREIDDLDEGGREEGKRLIPAEQFANAAVSALSVESLLHDLGTWKALKEENKNNINKNVFAFCRFPFLLSAEAKQRLLHGEAALGQQQAAQRDLMRQMFSGGPVSPFLVLRVDRARLLESTLAQIGVCDAMMLKKQLKVSFVGEEGVDEGGVKKEFFQLLCAQLFDFTYGMFRSTESGREVWFSPSHGPSFGSDDSQEYQLVGVLVGLAIYNSVLLDLHFPLVAYKKLLGLPVGFADLKELDAEMGEGLRKLLEWEEEGGREGGCGGGKVEDIFGLCFEVDWMEFGEVRRHELIEGGREIEVTAANRRDYVRLYVEWVLDGSIRKSFMDFKSGFDKVMGGSSISLLKPEELMLLVCGTPHMNLRELEAVARYEGGYDKSSNVIQWFWAVVHEMDLTSQRQLLMFVTGSAKAPIGGLGKLSLLIQRAGPESDHLPTAHTCFNVLLMPEYGSKEKLKERLMKAISECRGFGLQ 1414
            PTP  L    S    K++ ++ ++ YHT  +T  GEVY  G N+ GQ+     ++ +  P+ V  L +  V  ++CG  HT  +T +GA+ S+G +E GQLG  +G    G           V+PR V G       ++A G   T+ L+  G+V+S G G    G  G          +L+                                                                                     L          G+    G GT     +P  V QL G  +  ++ G  HT+ L   G+V+  G     QLGT  S     P +  S    +          H  +V    E                       +++  +        G  +D       R  S ++P    +    LL ++Q  AAS+     L   L DIFSS   L   F   ++ + +        R  S    S   GLD++ +++IY  V++L +  ++ +L  A    F     ++  V +SL++    + LL   QCPL           + L      +    + ++  W R  YP  +F  R +R +H  +  + +      R   + + ++  L A +     D +E+             L+P   F N ++S  +  SL  D   W  +K  N    +  + AFC+FPF+L+  AK ++L  EA + +    +  +M Q+FS    +PFL+L V R+ L+  TL Q+   D   LKK LKV F GE GVDEGGV KEFFQLL  +LF+  YGMF   E  R  WF+         +   E++LVG ++GLAIYN V+LD+HFP   YKKLL   V   DL++L+ ++ +GL +L  ++           G VE  F   F++ +  FGE++ ++L+E G  I VT  NR  YV LYV+++L+ SIR+ F  F  GF +V  G ++SL + EEL LL+CG PH +   LE V +YE GY K S VI+WFW VV  M L  ++QLL F TG+ +AP+GGL  L  +I R G ++D LPTAHTCFNVL++P Y SK KL+ RL  AI+   GFGLQ
Sbjct:   29 PTPTSL----STLVGKDIVHIAASDYHTAFLTDEGEVYTTGSNDAGQLGVRGMEEQV-VPIRVAALDTHMVTHIACGFKHTVAVTETGALASWGGDEYGQLGFDSGNIVDG-----------VQPRIVKGSRDLHFSRVAAGGYHTLALTGSGDVYSFGDGS--SGALGHGNLDGCSTPSLVKSLWGLGVTQIACGENHSAALTVDGKVFTWGRGKYGQLGHGSTQNIKLPVAVKALVDHDVIQIACGGDHTLAITSDGRLFSWGHGL---------WGQT---GHGTKEDVLSPKQVHQLEGRVLVQVSAGARHTVVLLDRGEVYGWGDCEQRQLGTCDSEIQFSPKLLFSVEDGKRPLFAVAGGEHTMVVC---EHIMEHQQGHLGIERQLLDTSSESSLVEGSATKPCLPGGKDVDN------RHGSGLRP----MKLPSLLQIVQNVAASSRAIPVLAHALEDIFSSVRFLIVVFTQKISRNINGDENQIIDRVHSRGEDSEGPGLDLELVQAIYHGVLQLLNPEIVRKLGEAMVRLFQGIEKYMDEVPESLWE----KVLLIGLQCPLIGENGPGDMISVHLFSIFHRISASAKRKMCKWLRT-YPKDIFGGRFVRGIHRFIS-NRETQLSSSRE-GIHSDVLGSLKALSILYEADSKEV-------------LVPYSDFYNTSIS--NHASLEDDYYRWSNVKFANN---DVPIVAFCQFPFILTPVAKSKILQLEANIQKTNQLRLSVMTQLFSAELQNPFLILTVRRSSLVADTLRQLEYEDD--LKKPLKVIFEGEAGVDEGGVTKEFFQLLIRELFNVGYGMFTYNEDTRHFWFNRD-----SMETKHEFRLVGNILGLAIYNGVILDIHFPKAVYKKLLRESVSLKDLRDLEPQIAKGLEELFVYQ-----------GDVESTFCQNFQITYEYFGEMKIYDLVEDGGNITVTNDNRERYVSLYVKYLLEDSIREQFDAFLEGFYQVCIGPALSLFRHEELELLICGLPHFDFDALERVTKYENGYTKDSQVIKWFWEVVKSMSLDEKKQLLFFTTGNDRAPVGGLATLKFIITRNGDDTDRLPTAHTCFNVLMVPNYSSKSKLENRLKLAIANSTGFGLQ 1074          

BLAST of NO04G01130 vs. NCBI_GenBank
Match: XP_002508077.1 (E3 ubiquitin-protein ligase [Micromonas commoda] >ACO69335.1 E3 ubiquitin-protein ligase [Micromonas commoda])

HSP 1 Score: 400.6 bits (1028), Expect = 2.000e-107
Identity = 354/1225 (28.90%), Postives = 531/1225 (43.35%), Query Frame = 0

Query:  291 PTPPLLKPTKSKYGKKNVANLCSTLYHTVAVTTTGEVYGCG-VNEDGQV--SPDKEDQYLWAP--VLVERLLSQRVIQVSCGENHTACLTSSGAVLSFGNNEVGQLGHSAGATTTGEIVRSSRGSRRVE----PRTVVGLGGKLIKQIACGYLFTVMLSSEGEVFSCGVG--GCVGGQDGKDRFQAERVDALMFVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLPPCPSVSPVPGEEELGGGGTVSPYRAPVLVSQ--LNGLEVCMLACGDAHTLALTKSGQVWSMGANSSGQLGTG-ASATNAEPSIHPSSSSWRA-----TEVQGLQTAH-VFMVAAGGEQSFAXXXXXXXXXXXXXXXXXXKTIIGTAVPTTAATPGLSLDESTLDLIRKFSVIKPATSFLTTAQLLTLIQQAASTHQ--PDFLERTLFDIFSSPSLLNGSFLSVAHSSSLTAKSSTXRSCSTSTLSGLDVQGLESIYVAVMKLNHTPLLSRLMHAFSTALDHLLPVAK----SLYDPDSLRCLLA----YWQCPLNSNTSLSRDTVLKLCEAIVSLPGDGRVQ-LFAWARADYPPHLFATRLLRPLHSHLEYHLKVNFGK---------------GRAVP---------MLAVIMNYLYAANERIREDGREIDDLDEGGREEGKRLIPAEQFANAAVS-----------ALSVESLLHDLGTWKALKEENKNNINK--NVFAFCRFPFLLSAEAKQRLLHGEAALGQQQAAQRD----LMRQMFS-----GGPVSPFLVLRVDRARLLESTLAQIGVCDAMMLKKQLKVSF----VGEEGVDEGGVKKEFFQLLCAQLF-------------------DFTYGMFRSTESGREVWFSPSHGPSFGSDDSQEYQLVGVLVGLAIYNSVLLDLHFPLVAYKKLLGLPVGFADLKELDAEMGEGLRKLLEWEEEGGREGGCGGGKVEDIFGLCFEVDWM--EFGEVRRHELIEGGREIEVTAANRRDYVRLYVEWVLDGSIRKSFMDFKSGFDKVMGGSSISLLKPEELMLLVCGTPHMNLRELEAVARYEGGYDKSSNVIQWFWAVVHEMDLTSQRQLLMFVTGSAKAPIGGLGKLSLLIQRAGPESDHLPTAHTCFNVLLMPEYGSKEKLKERLMKAISECRGFGLQ 1414
            PTP     + S    ++V ++     H+VAVT+ GEVY  G  + +G+   +P +  + +  P  V        RV  V+CG+ H A +T+SG +LS+GN E GQLG      ++ +    S   RR +    PR +VG G      +ACG   T+ L++ G VFS G G  G +G  D  +      VDAL  V                                                                                              G     G G  +    P ++ +  L   +V  ++ G  H++A+T+ G V++ GA + GQLG     A  +E  +   S+   A      +V GL     V    A G+ +FA                        A  T    P       +L  I     + P      +   L     A   H+  P+ + R + D+FSSP  +   F          A      S S +  SGLDV  +E++Y A+       +++ L +A +  L+ LL   +     L D +  R ++A     W  PLN + +L    V +L      L   G    L  W  AD    +F  RL+ PL   L   L  +  +               GR  P           A ++  L+AANE IR+            ++ G+ ++PA  F + ++S            + +++     GTW   +  +     +   +  FC+ PFLL+AEAK R+L GEA L ++   +      +    FS     G P +PFL L ++R  LL++ L  +       LKK L++ F    V EEGVDEGGV KEFFQL+  ++F                        MF   E  R  WF+P+   S  ++    ++L G  +GLAIYN V LD+H P VAY++L G+     DL EL   +GEGL+ LL+++          G  +E++F   F V+    E G     EL  GG    VT  NR+++V+LYV+  LD S+   F  FK+GF++V GG ++ L  P EL LLVCG P ++++ LE V +Y+GG+D     I+ FW+VVH + +  Q++LL F TG  +AP+GGL  L  ++QR+GP+++HLPTAHTCFNVLL+PEY  +EKL++RL  AI+   GFGLQ
Sbjct:  197 PTP----RSVSSLDGRDVTHVAGGARHSVAVTSAGEVYAFGDCSGEGKALSAPTRLREAVIDPPEVTATTNAMPRVATVACGQRHCAAVTTSGVLLSWGNEEFGQLGQGPPRDSSSKGDAKSLIRRRADASMMPR-LVGSGHVKFTSVACGAGHTLALTANGRVFSFGRGAFGALGHGDRLNCDVPRLVDALWGVGVAQIAAGDNHSASLSASGRVYTWGRGKYGALGHRDVDNRSRPTPVQALDDAGVRCEQIACGGDHTLAIAGGGSGVLLAWGR------------GSSGPAGTGATADVLTPTIIDRALLGDEKVFQISAGSKHSVAVTEGGCVYTWGARTQGQLGHELGDAGTSEHGVWKLSTGGGAAVSAVAKVAGLPAGRDVLYAVAAGDHTFAAVAPGKG-----------------AATTRGRAPWSGAHGWSLRAIE----LPPILELAESITALDPEVDADECHRIVPELI-RAVEDVFSSPGFIVEGF---------AAPRFAEESESDARTSGLDVTSVEAVYSALCSCGRPEIIAALANASAKLLEALLDALQRAKTDLEDVNDTRVVVAAMIVLWMSPLNGDPALGDTLVPRLVAVTEQLTATGVGDLLIPWLAADIDADVFERRLVSPLMGFLTRSLGKDLARKHHKGRGLVGSIGPSGRTSPGGGMDVFAVRAAQMLGMLHAANE-IRQ------------KQTGEPIVPASVFYSQSLSDSLNLREEYMRLIQLDADGLGTGTWGGDRMNSYPACLRPGELVTFCQLPFLLTAEAKGRILQGEANLQKRHEMRMSHLSAIQNGQFSNDLPFGSPDAPFLELVIERGNLLKNALDAVASKTPADLKKPLRIKFKSDGVEEEGVDEGGVTKEFFQLMVREMFKDARTFVEFSEHGSEDKNSSLAPPMFTLDEESRYHWFNPA--ASVSAESLARFRLFGAALGLAIYNGVCLDVHLPPVAYRRLCGMEPTLVDLCELSPSLGEGLQTLLDYD----------GDDIEEVFYRDFVVERFDAETGRTLTTELKPGGEHTAVTGGNRKEFVKLYVQHALDKSVSAQFGAFKAGFEQVCGGPALGLFTPTELELLVCGDPEIDMKALERVTKYDGGFDADHRAIRDFWSVVHSLPIADQKRLLFFATGCDRAPVGGLENLPFVVQRSGPDTEHLPTAHTCFNVLLLPEYDCQEKLRDRLAVAIANAEGFGLQ 1348          

BLAST of NO04G01130 vs. NCBI_GenBank
Match: XP_005704626.1 (ubiquitin-protein ligase E3 [Galdieria sulphuraria] >EME28106.1 ubiquitin-protein ligase E3 [Galdieria sulphuraria])

HSP 1 Score: 400.2 bits (1027), Expect = 2.600e-107
Identity = 330/1154 (28.60%), Postives = 515/1154 (44.63%), Query Frame = 0

Query:  306 KNVANLCSTLYHTVAVTTTGEVYGCGVNEDGQVS-PDKEDQYLWAPVLVERLLSQRVIQVSCGENHTACLTSSGAVLSFGNNEVGQLGHSAGATTTGEIVRSSRGSRRVEPRTVVGLGGKLIKQIACGYLFTVMLSSEGEVFSCGVGGCVGGQDGKDRFQAERVDALMFVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLPPCPSVSPVPGEEELGGGGTVSPYRAPVLVSQLNGLEVCMLACGDAHTLALTKSGQVWSMGANSSGQLG-TGASATNAEPSIHPSSSSWRATEVQGLQTAHVFM-VAAGGEQSFAXXXXXXXXXXXXXXXXXXKTIIGTAVPTTAATPGLSLDESTLDLIRKFSVIKPATSFLTTAQLLTLIQQ--AASTHQPDFLERT-------------------LFDIFSSPSLLNGSFLSVAHSSSLTAKSSTXRSCSTSTLSGLDVQGLESIYVAVMKL---NHTPLLSRLMHAFSTALDHLLPV---AKSLYDPDSLRCLLAYWQCPLNSNTSLSRDT----VLKLCEAIVSLPGDGRVQLFAWARADYPPHLFATRLLRPLHSHLEYHL-KVNFGKGRAVPMLAVIMNYLYAANERIREDGREIDDLDEGGREEGKRLIPAEQFANAAVSALSVESLLHDLGTWKALKEENKNNINKNVFAFCRFPFLLSAEAKQRLLHGEAALGQQQAAQRDLMRQM-----------FSGGPVSPFLVLRVDRARLLESTLAQIGVCDAMMLKKQLKVSFVGEEGVDEGGVKKEFFQLLCAQLFDFTYGMFRSTESGREVWFSPSHGPSFGSDDSQEYQLVGVLVGLAIYNSVLLDLHFPLVAYKKLLGLPVGFADLKELDAEMGEGLRKLLEWEEEGGREGGCGGGKVEDIFGLCFEVDWMEFGEVRRHELIEGGREIEVTAANRRDYVRLYVEWVLDGSIRKSFMDFKSGFDKVMGGSSISLLKPEELMLLVCGTPHMNLRELEAVARYEGGYDKSSNVIQWFWAVVHEMDLTSQRQLLMFVTGSAKAPIGGLGKLSLLIQRAGPESDHLPTAHTCFNVLLMPEYGSKEKLKERLMKAISECRGFGLQ 1414
            K V  + +  +H +A+T  G+VY CG+   GQ    + ED Y   P LVE L +Q V+ ++ GENH+  +TS+G   +FG+N+ GQLG S       E  R+    R   P+    L  +     ACG   T+ L+S G+V +CG      GQDG                                                                                                                 + G G        V++  L    +  +A G  H+ AL+  G+++  G+N  GQLG    +   + P+   ++  ++ +  +   +++ F+ +A G   +                      +IG      A++P         ++   + +   +  + +   LL L ++  AAS     FL  T                   +F   +  + +  +   V  ++S    S+   S  +     +    +ES Y  ++ +   N   LL+ +     +  +++L      K+     S    +      L S  S  + T    + +LC  I SL    + ++ + A + +P  L A RL+RPLHS ++  L +V     RA+    V +   YAA+E              G R     ++P ++F N +VS L    L  D   W  L     ++  K++F+FC   ++L   AK R+LH EA    +    R L               F  G  SP+  L V RA L++  L+ I   D   L+K L+V F+GEEGVDEGG++KEFFQL+ A+LF   YGMF   E  R  WF      S   DD+  ++LVG+++G+A+YN V+LDLHFPL+ YKKLL       D+KE   E+G  L+ +L++EE+           +E +F   F + +  FGE+R  EL+ GG +I V  +N+ D++R Y+E++ D SI   F  F  GF  ++ G ++ + +P+EL +LVCG  H++   L+ V  YEGG++  S VI+WFW +V EM L  +  LL FVTGS +AP+GGLG+L  +IQRAGP++D LPT+HTCFN+LL+PEY SKEK + RL+ AI   +GFGLQ
Sbjct:  154 KRVVKVAAGAFHWLALTDAGQVYSCGLGRHGQTGLGNCEDVY--EPCLVEALWAQPVVNIAAGENHSIVITSTGFAFAFGSNKYGQLGISRQRLPESE-TRAELPLRIYMPQE---LASERFLAAACGTHHTLFLTSNGKVLACG-----SGQDG-----------------------------------------------------------------------------------------------------------------VLGIGNNKDEVCLVVIFSLFEYTIVQIAAGARHSAALSSQGELFLWGSNEQGQLGFVEPTCVFSLPTQMDTNIIFKDSFEKEESSSYFFLKIATGAYHTIG--------------------LIGKEKEKCASSP--------FEIYPSYLMNLTSMQWSSVRDLLPLQKRLSAASIFSHSFLYGTVTGTDSSEKHGRWRSINPYIFPFINWNTAIGKNNNQVNENASYVQDSNLLTSVVSKPELFIQATAVESFYKELLSVHGSNAAELLTFISKEIISQAENMLTFRIREKAEQGNHSTTVAIESTGSDLKSKVSSRQGTGKQLLTRLCLIIASLKLGAQRRIVS-AVSRFPAELVAVRLVRPLHSIIDSELNEVRRITERAIAATRV-LGLFYAASEL------------NGWR---YAILPRQEFYNHSVSDL--VDLKEDYNRW--LYSMENSSSGKSLFSFCAHSYVLDEAAKARVLHAEAREAMKSEVVRSLWMNSQAVVVGPWGIPFIAGTDSPYCYLHVRRASLVQDALSVISNADPRELRKPLRVVFLGEEGVDEGGLQKEFFQLITAELFSADYGMFVYNEKTRFHWFRVD---SLEVDDN--FKLVGIIIGMALYNGVILDLHFPLLVYKKLLNWSPDLDDVKEAFPEIGNSLQAMLDYEED----------DMEQVFMTTFAIQYEFFGELRTWELVPGGIQIYVNQSNKEDFIRRYIEYLTDVSIHSQFDAFSKGFFWMLSGPALKIFRPDELEVLVCGQRHLDFEALKQVTVYEGGFNAESQVIKWFWDIVDEMTLEEKLHLLFFVTGSDRAPVGGLGQLRFVIQRAGPDTDRLPTSHTCFNILLLPEYSSKEKTRNRLLTAIQNAQGFGLQ 1119          

BLAST of NO04G01130 vs. NCBI_GenBank
Match: XP_024376936.1 (probable E3 ubiquitin-protein ligase HERC4 isoform X2 [Physcomitrella patens])

HSP 1 Score: 396.7 bits (1018), Expect = 2.900e-106
Identity = 321/1073 (29.92%), Postives = 475/1073 (44.27%), Query Frame = 0

Query:  350 PVLVERLLSQRVIQVSCGENHTACLTSSGAVLSFGNNEVGQLGHSAGATTTGEIVRSSRGSRRVEPRTVVGLGGKLIKQIACGYLFTVMLSSEGEVFSCGVGGCVGGQDGKDRFQAERVDALMFVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLPPCPSVSPVPGEEELGGGGTVSPYRAPVLVSQLNGLEVCMLACGDAHTLALTKSGQVWSMGANSSGQLGTGASATNAEPSIHPSSSSWRATEVQGLQTAHVFMVAAGGEQSFAXXXXXXXXXXXXXXXXXXKTIIGTAVPTTAATPGLSLDESTLDLIRKFSVIKPATSFLTTAQLLTLIQQ-AASTHQPDFLERTLFDIFSSPSLLNGSFL-SVAHSSSLTAKSSTXRSCSTSTLS---GLDVQGLESIYVAVMKLNHTPLLSRLMHA----FSTALDHLLPVAKSLYDPDSLRCLLAYWQCPLNSNTSLSRDTVLKLCEAIVSLPGDGRVQLFAWARADYPPHLFATRLLRPLHSHLEYHLKVNFGKGRAVPMLAVIMNYLYAANERIREDGREIDDLDEGGREEGKRLIPAEQFANAAVSALSVESLLHDLGTWKALKEENKNNINKNVFAFCRFPFLLSAEAKQRLLHGEAALGQQQAAQRDLMRQMFSGGPVSPFLVLRVDRARLLESTLAQIGVCDAMMLKKQLKVSFVGEEGVDEGGVKKEFFQLLCAQLFDFTYGMFRSTESGREVWFSPSHGPSFGSDDSQEYQLVGVLVGLAIYNSVLLDLHFPLVAYKKLLGLPVGFADLKELDAEMGEGLRKLLEWEEEGGREGGCGGGKVEDIFGLCFEVDWMEFGEVRRHELIEGGREIEVTAANRRDYVRLYVEWVLDGSIRKSFMDFKSGFDKVMGGSSISLLKPEELMLLVCGTPHMNLRELEAVARYEGGYDKSSNVIQWFWAVVHEMDLTSQRQLLMFVTGSAKAPIGGLGKLSLLIQRAGPESDHLPTAHTCFNVLLMPEYGSKEKLKERLMKAISECRGFGLQ 1414
            P+ V  L +  V  ++CG  HT  +T +GA+ S+G +E GQLG  +G    G           V+PR V G       ++A G   T+ L+  G+V+S G G    G  G          +L+                                                                                     L          G+    G GT     +P  V QL G  +  ++ G  HT+ L   G+V+  G     QLGT  S     P +  S    +          H  +V    E                       +++  +        G  +D       R  S ++P    +    LL ++Q  AAS+     L   L DIFSS   L   F   ++ + +        R  S    S   GLD++ +++IY  V++L +  ++ +L  A    F     ++  V +SL++    + LL   QCPL           + L      +    + ++  W R  YP  +F  R +R +H  +  + +      R   + + ++  L A +     D +E+             L+P   F N ++S  +  SL  D   W  +K  N    +  + AFC+FPF+L+  AK ++L  EA + +    +  +M Q+FS    +PFL+L V R+ L+  TL Q+   D   LKK LKV F GE GVDEGGV KEFFQLL  +LF+  YGMF   E  R  WF+         +   E++LVG ++GLAIYN V+LD+HFP   YKKLL   V   DL++L+ ++ +GL +L  ++           G VE  F   F++ +  FGE++ ++L+E G  I VT  NR  YV LYV+++L+ SIR+ F  F  GF +V  G ++SL + EEL LL+CG PH +   LE V +YE GY K S VI+WFW VV  M L  ++QLL F TG+ +AP+GGL  L  +I R G ++D LPTAHTCFNVL++P Y SK KL+ RL  AI+   GFGLQ
Sbjct:    7 PIRVAALDTHMVTHIACGFKHTVAVTETGALASWGGDEYGQLGFDSGNIVDG-----------VQPRIVKGSRDLHFSRVAAGGYHTLALTGSGDVYSFGDGS--SGALGHGNLDGCSTPSLVKSLWGLGVTQIACGENHSAALTVDGKVFTWGRGKYGQLGHGSTQNIKLPVAVKALVDHDVIQIACGGDHTLAITSDGRLFSWGHGL---------WGQT---GHGTKEDVLSPKQVHQLEGRVLVQVSAGARHTVVLLDRGEVYGWGDCEQRQLGTCDSEIQFSPKLLFSVEDGKRPLFAVAGGEHTMVVC---EHIMEHQQGHLGIERQLLDTSSESSLVEGSATKPCLPGGKDVDN------RHGSGLRP----MKLPSLLQIVQNVAASSRAIPVLAHALEDIFSSVRFLIVVFTQKISRNINGDENQIIDRVHSRGEDSEGPGLDLELVQAIYHGVLQLLNPEIVRKLGEAMVRLFQGIEKYMDEVPESLWE----KVLLIGLQCPLIGENGPGDMISVHLFSIFHRISASAKRKMCKWLRT-YPKDIFGGRFVRGIHRFIS-NRETQLSSSRE-GIHSDVLGSLKALSILYEADSKEV-------------LVPYSDFYNTSIS--NHASLEDDYYRWSNVKFANN---DVPIVAFCQFPFILTPVAKSKILQLEANIQKTNQLRLSVMTQLFSAELQNPFLILTVRRSSLVADTLRQLEYEDD--LKKPLKVIFEGEAGVDEGGVTKEFFQLLIRELFNVGYGMFTYNEDTRHFWFNRD-----SMETKHEFRLVGNILGLAIYNGVILDIHFPKAVYKKLLRESVSLKDLRDLEPQIAKGLEELFVYQ-----------GDVESTFCQNFQITYEYFGEMKIYDLVEDGGNITVTNDNRERYVSLYVKYLLEDSIREQFDAFLEGFYQVCIGPALSLFRHEELELLICGLPHFDFDALERVTKYENGYTKDSQVIKWFWEVVKSMSLDEKKQLLFFTTGNDRAPVGGLATLKFIITRNGDDTDRLPTAHTCFNVLMVPNYSSKSKLENRLKLAIANSTGFGLQ 998          

BLAST of NO04G01130 vs. NCBI_GenBank
Match: PRW59408.1 (ubiquitin- ligase E3 [Chlorella sorokiniana])

HSP 1 Score: 395.6 bits (1015), Expect = 6.500e-106
Identity = 353/1194 (29.56%), Postives = 497/1194 (41.62%), Query Frame = 0

Query:  306 KNVANLCSTLYHTVAVTTTGEVYGCGVNEDGQVSPDKEDQYLWAPVLVERLLSQRVIQVSCGENHTACLTSSGAVLSFGNNEVGQLGHSAGATTTGEIVRSSRGSRRVEPRTVVGLGGKLIKQIACGYLFTVMLSSEGEVFSCGVGGCVGGQDGKDRFQAERVDALMFVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLPPCPSVSPV-PGEEELGGGGTVSPYRAPVLVSQLNGLEVCMLACGDAHTLALTKSGQVWSMGANSSGQLGTGASATNAEPSIHPSSSSWRATEVQGLQ-TAHVFMVAAGGEQSFAXXXXXXXXXXXXXXXXXXKTIIGTAVPTTAATPGLSLDESTLDLIRKFSVIKPATSFLTTAQLLTLIQQAASTH----QPDFL--------------------------------------------ERTLFDIFSSPSLLNGSFLSVAHSSSLTAKSSTXRSCSTSTLSGLDVQGLESIYVAVM---------KLNHT-----PLLSRLMHAFSTALDHLLPVAKSLYDPDSLR----------CLLAYWQCPLNSNTSLSRDTVLKLCEAI--VSLPGDGRV---QLFAWARADYPPHLFATRLLRPLHSHLEYHLKVNFGKGRA-VPMLAVIMNYLYAANERIREDGREIDDLDEGGREEGKRLIPAEQFANAAVSALSVESLLHDLGTWKALKEENKNNINKNVFAFCRFPFLLSAEAKQRLLHGEAALGQQQAAQRDLMRQMFSGGPVS--PFLVLRVDRARLLESTLAQIGVCDAMMLKKQLKVSF----VGEEGVDEGGVKKEFFQLLCAQLFDFTYGMFRSTESGREVWFSPSHGPSFGSDDSQEYQLVGVLVGLAIYNSVLLDLHFPLVAYKKLLGLPVGFADLKELDAEMGEGLRKLLEWEEEGGREGGCGGGKVEDIFGLCFEVDWMEFGEVRRHELIEGGREIEVTAANRRDYVRLYVEWVLDGSIRKSFMDFKSGFDKVMGGSSISLLKPEELMLLVCGTPHMNLRELEAVARYEGGYDKSSNVIQWFWAVVHEMDLTSQRQLLMFVTGSAKAPIGGLGKLSLLIQRAGPESDHLPTAHTCFNVLLMPEYGSKEKLKERLMKAISECRGFGLQ 1414
            +N+ NL +  + T  VT  GE+Y  G N+  Q+   ++ + +  P  V+ L S++V QV CG  HT  L   G V +FG+NE GQ G              S G++   PR V  L    I ++A G   T++LSS G VFSCG   C  GQ G+D  +   V                                                                                         L    SV  +  G     G   +     P  V+ L G  +  +A G  HTLAL  +  +W+ G N SGQL   A+     P +           VQGL     +  V+AGG+ S A                      G   P      G       L  + + +    AT     A+   L     +T      P +L                                                                             X        +GLDVQ + + Y A++          L HT      +L R +++ +   D+    A      D+ R           L    Q PLNS+TS     VL     I  V+LP   R+   Q      A  P    A R +RP+  HL+  +    G  R  V M  V+++ +  AN                  E+   LIP  +F N ++S  +  +L  +   W+      +N     + + C+ P+LL+ EAK R++ GEAA+ QQQ      M+ +F G   S   +L + + R  ++E  L Q+ V  A  LKK L+V+F    V E   DEGGV KEFF LL   LF   Y MF   E+ R  WF+ S       +   EY LVG ++GLAIYN  +LD+HFP+V YKKLLG   GF DLK    ++G GL++LL+++           G VE +F   F  +W  + E+R  EL  GG  I VT  NR++YV+LY EW+L+ SI + F  F+ GF +V  G ++ L  P EL LL+CG PH++   LE  A+YE GY ++  V+ WFW V+H   L  +R  L F TGS +AP+GGLG+L LLIQRAG +++HLPTAHTCFN LL+PEY SKEKL+++L+ AI   +GFGL+
Sbjct:  136 RNILNLAAGAFDTAVVTADGELYTAGSNDSSQLGL-RQQECVPDPTRVQGLESRQVEQVVCGALHTLALIDDGTVAAFGDNEFGQTG------------LESSGTQVALPRVVKDLHSMHIMRVAAGGAHTLLLSSTGGVFSCGSAAC--GQLGRDTSKGAEV-------------LRPVSRLWPLGVVQVAAGDAHSAALLADGRLLTWGRGKYGALGLGDFDNRSVPTLVRSMAGVAIRQVSCGDDHTVLLAEDGSVYTMGRGTWGQTGHSHLDNTCLPQRVTALAGQRIVQVAAGGRHTLALADNNMLWACGNNESGQLAAQANTAQPTPQV-----------VQGLPGGCPILFVSAGGDHSVAVVDRHASLE------------AGPIGPCADRPHGEQWCAVQLPDLLQLAAEAAATKGDPAARKAKLEAVMVATECVFASPGYLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAGLDVQRIAATYQALLTLYDADVATALGHTCIRLLDMLERFLNSLTQQQDYATATAGDGPSGDAARRRQDLSWVAPVLFILLQNPLNSDTSGFGSKVLVRISRIVGVTLPARERLPVRQALQRLLARLPADALAARCVRPVQRHLDACVAQGLGATRVQVLMAGVLLDVMRQAN------------------EDAGELIPYSEFHNRSLSEHA--NLQAEYVLWR------QNQDPTALCSLCQMPYLLTPEAKSRIIQGEAAMEQQQHLSAAAMQVLFQGLNPSAVAYLHIHMRRTHVVEDALNQL-VYRADDLKKPLRVTFVTGGVPEPAQDEGGVTKEFFMLLVRDLFKPEYAMFSYDEATRTFWFNLS-----SLESEIEYALVGAVLGLAIYNGQVLDVHFPMVVYKKLLGQRPGFRDLKAAFPDLGRGLQQLLDFQ-----------GDVEAVFCRTFTAEWKFWDELRTEELKPGGASIPVTNDNRQEYVQLYTEWLLERSIAQQFNAFRKGFLRVCNGPALGLFTPAELELLICGLPHLDFEALERAAKYE-GYTRAHPVVCWFWQVLHSFSLEQKRAWLQFTTGSDRAPVGGLGRLPLLIQRAGADTEHLPTAHTCFNALLLPEYSSKEKLRQKLLLAIGWAQGFGLK 1234          

BLAST of NO04G01130 vs. NCBI_GenBank
Match: XP_024376937.1 (probable E3 ubiquitin-protein ligase HERC3 isoform X3 [Physcomitrella patens])

HSP 1 Score: 391.3 bits (1004), Expect = 1.200e-104
Identity = 318/1061 (29.97%), Postives = 469/1061 (44.20%), Query Frame = 0

Query:  362 IQVSCGENHTACLTSSGAVLSFGNNEVGQLGHSAGATTTGEIVRSSRGSRRVEPRTVVGLGGKLIKQIACGYLFTVMLSSEGEVFSCGVGGCVGGQDGKDRFQAERVDALMFVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLPPCPSVSPVPGEEELGGGGTVSPYRAPVLVSQLNGLEVCMLACGDAHTLALTKSGQVWSMGANSSGQLGTGASATNAEPSIHPSSSSWRATEVQGLQTAHVFMVAAGGEQSFAXXXXXXXXXXXXXXXXXXKTIIGTAVPTTAATPGLSLDESTLDLIRKFSVIKPATSFLTTAQLLTLIQQ-AASTHQPDFLERTLFDIFSSPSLLNGSFL-SVAHSSSLTAKSSTXRSCSTSTLS---GLDVQGLESIYVAVMKLNHTPLLSRLMHA----FSTALDHLLPVAKSLYDPDSLRCLLAYWQCPLNSNTSLSRDTVLKLCEAIVSLPGDGRVQLFAWARADYPPHLFATRLLRPLHSHLEYHLKVNFGKGRAVPMLAVIMNYLYAANERIREDGREIDDLDEGGREEGKRLIPAEQFANAAVSALSVESLLHDLGTWKALKEENKNNINKNVFAFCRFPFLLSAEAKQRLLHGEAALGQQQAAQRDLMRQMFSGGPVSPFLVLRVDRARLLESTLAQIGVCDAMMLKKQLKVSFVGEEGVDEGGVKKEFFQLLCAQLFDFTYGMFRSTESGREVWFSPSHGPSFGSDDSQEYQLVGVLVGLAIYNSVLLDLHFPLVAYKKLLGLPVGFADLKELDAEMGEGLRKLLEWEEEGGREGGCGGGKVEDIFGLCFEVDWMEFGEVRRHELIEGGREIEVTAANRRDYVRLYVEWVLDGSIRKSFMDFKSGFDKVMGGSSISLLKPEELMLLVCGTPHMNLRELEAVARYEGGYDKSSNVIQWFWAVVHEMDLTSQRQLLMFVTGSAKAPIGGLGKLSLLIQRAGPESDHLPTAHTCFNVLLMPEYGSKEKLKERLMKAISECRGFGLQ 1414
            +Q++CGENH+A LT  G V ++G  + GQLGH  G+T   ++           P  V  L    + QIACG   T+ ++S+G +FS G G  + GQ G                                                                                                                     GT     +P  V QL G  +  ++ G  HT+ L   G+V+  G     QLGT  S     P +  S    +          H  +V    E                       +++  +        G  +D       R  S ++P    +    LL ++Q  AAS+     L   L DIFSS   L   F   ++ + +        R  S    S   GLD++ +++IY  V++L +  ++ +L  A    F     ++  V +SL++    + LL   QCPL           + L      +    + ++  W R  YP  +F  R +R +H  +  + +      R   + + ++  L A +     D +E+             L+P   F N ++S  +  SL  D   W  +K  N    +  + AFC+FPF+L+  AK ++L  EA + +    +  +M Q+FS    +PFL+L V R+ L+  TL Q+   D   LKK LKV F GE GVDEGGV KEFFQLL  +LF+  YGMF   E  R  WF+         +   E++LVG ++GLAIYN V+LD+HFP   YKKLL   V   DL++L+ ++ +GL +L  ++           G VE  F   F++ +  FGE++ ++L+E G  I VT  NR  YV LYV+++L+ SIR+ F  F  GF +V  G ++SL + EEL LL+CG PH +   LE V +YE GY K S VI+WFW VV  M L  ++QLL F TG+ +AP+GGL  L  +I R G ++D LPTAHTCFNVL++P Y SK KL+ RL  AI+   GFGLQ
Sbjct:   10 LQIACGENHSAALTVDGKVFTWGRGKYGQLGH--GSTQNIKL-----------PVAVKALVDHDVIQIACGGDHTLAITSDGRLFSWGHG--LWGQTGH--------------------------------------------------------------------------------------------------------------------GTKEDVLSPKQVHQLEGRVLVQVSAGARHTVVLLDRGEVYGWGDCEQRQLGTCDSEIQFSPKLLFSVEDGKRPLFAVAGGEHTMVVC---EHIMEHQQGHLGIERQLLDTSSESSLVEGSATKPCLPGGKDVDN------RHGSGLRP----MKLPSLLQIVQNVAASSRAIPVLAHALEDIFSSVRFLIVVFTQKISRNINGDENQIIDRVHSRGEDSEGPGLDLELVQAIYHGVLQLLNPEIVRKLGEAMVRLFQGIEKYMDEVPESLWE----KVLLIGLQCPLIGENGPGDMISVHLFSIFHRISASAKRKMCKWLRT-YPKDIFGGRFVRGIHRFIS-NRETQLSSSRE-GIHSDVLGSLKALSILYEADSKEV-------------LVPYSDFYNTSIS--NHASLEDDYYRWSNVKFANN---DVPIVAFCQFPFILTPVAKSKILQLEANIQKTNQLRLSVMTQLFSAELQNPFLILTVRRSSLVADTLRQLEYEDD--LKKPLKVIFEGEAGVDEGGVTKEFFQLLIRELFNVGYGMFTYNEDTRHFWFNRD-----SMETKHEFRLVGNILGLAIYNGVILDIHFPKAVYKKLLRESVSLKDLRDLEPQIAKGLEELFVYQ-----------GDVESTFCQNFQITYEYFGEMKIYDLVEDGGNITVTNDNRERYVSLYVKYLLEDSIREQFDAFLEGFYQVCIGPALSLFRHEELELLICGLPHFDFDALERVTKYENGYTKDSQVIKWFWEVVKSMSLDEKKQLLFFTTGNDRAPVGGLATLKFIITRNGDDTDRLPTAHTCFNVLMVPNYSSKSKLENRLKLAIANSTGFGLQ 883          

BLAST of NO04G01130 vs. NCBI_GenBank
Match: XP_004357184.1 (o ubiquitin protein ligase E3A [Acanthamoeba castellanii str. Neff] >ELR25029.1 o ubiquitin protein ligase E3A [Acanthamoeba castellanii str. Neff])

HSP 1 Score: 384.8 bits (987), Expect = 1.200e-102
Identity = 249/699 (35.62%), Postives = 361/699 (51.65%), Query Frame = 0

Query:  725 ATSFLTTAQLLTLIQQAASTHQPDFLERTLFDIFSSPSLLNGSFLSVAHSSSLTAKSSTXRSCSTSTLSGLDVQGLESIYVAVMKLNHTPLLSRLMHAFSTALDHLLPVAKSLYDPDSLRCLLAYWQCPLNSNTSLSRDTVLKLCEAIVSLPGDGRVQLFAW----------ARADYPPHLFATRLLRPLHSHLEYHLKVNFGKGRAVPMLAVIMNYLYAANERIREDGREIDDLDEGGREEGKRLIPAEQFANAAVSALSVESLLHDLGTWKALKEENKNNI-NKNVFAFCRFPFLLSAEAKQRLLHGEAALGQQQAAQRDLMRQMFSGGPVSPFLVLRVDRARLLESTLAQIGVCDAMMLKKQLKVSFVGEEGVDEGGVKKEFFQLLCAQLFDFTYGMFRSTESGREVWFSPSHGPSFGSDDSQEYQLVGVLVGLAIYNSVLLDLHFPLVAYKKLLGLPVGFADLKELDAEMGEGLRKLLEWEEEGGREGGCGGGKVEDIFGLCFEVDWMEFGEVRRHELIEGGREIEVTAANRRDYVRLYVEWVLDGSIRKSFMDFKSGFDKVMGGSSISLLKPEELMLLVCGTPHMNLRELEAVARYEGGYDKSSNVIQWFWAVVHEMDLTSQRQLLMFVTGSAKAPIGGLGKLSLLIQRAGPESDHLPTAHTCFNVLLMPEYGSKEKLKERLMKAISECRGFGL 1413
            AT  L+  ++  L+  A  +     + RT   +FS P  LN SFL          K+      +T   SGLD   +  ++  V ++    + + L  AF   +  L   A  L  P+SLR ++   Q P+  +    +  +  L  AI  LP   +  L +W                      R+L   H+        +      +P    ++  LY ANERI +D                  +   +F N A++    ES+         LKE+    I N   F+FC + F+  A  K ++L  E+ + Q    Q  L R +F+G   +P LV ++ R  L++ TL+ I   D   LKK+L+V FVGEE +DEGGV+KE FQL+  Q+ D  +GMF  +E  R  WF+        S D  E++L+G+++GLAIYN ++LDLHFP V YKKL+GL     DLK+++     GLRKLLE++           G VE+ F   F++ +  +G V+ H+L EGG +I +T  NR++YV LYV W L  SI   F  F SGF  V    +  + + EEL  LVCG+P ++  ELE V +Y+ GY +   VI+ FW++VH M L  +++LL F TGS ++PIGGLGKL+ +I R G +SD LP AHTCFN LL+PEY S+EKL+ERLM AIS   GFG+
Sbjct:   64 ATPHLSIQKMRELVVNAKQSSDYTPIIRTFGSVFSEPEFLNVSFL----------KTGDYAVAATPESSGLDENAVAELFAEVEEM-PANVRNVLASAFERLVASLKVAAPRLSQPESLRQVVIVLQSPMLLDLDYHKSILGPLIRAIPLLPERSKDVLVSWWSQMSAERMRTFLGIVQQYITLRILAAPHA-------FSLHNDDYIPAGVRVLQLLYTANERITKD-----------------KLDYPEFYNDAIN----ESI--------DLKEDFMRWIDNSGQFSFCNYSFVFDAGTKSKILQYESIVEQHHQRQEALRRTIFTGMVSAPALVFKIRREHLIQDTLSAIQRHDPADLKKELRVHFVGEEAIDEGGVQKELFQLILRQILDAKFGMFTYSEEARTSWFNSV------STDFDEFKLIGIILGLAIYNGIILDLHFPFVVYKKLVGLEPTLDDLKDVN----PGLRKLLEFD-----------GNVEETFMQNFQISYEAYGMVQTHDLKEGGADIPLTNDNRQEYVDLYVRWYLKDSIAAQFEAFLSGFRLVCESRAFDMFRAEELEQLVCGSPVLDFEELEKVTQYDNGYHQDHRVIKDFWSIVHSMTLEEKKRLLFFSTGSDRSPIGGLGKLAFVITRHGEDSDRLPQAHTCFNHLLLPEYASREKLQERLMTAISNAEGFGM 694          

BLAST of NO04G01130 vs. NCBI_GenBank
Match: GAX80909.1 (hypothetical protein CEUSTIGMA_g8344.t1 [Chlamydomonas eustigma])

HSP 1 Score: 381.3 bits (978), Expect = 1.300e-101
Identity = 350/1281 (27.32%), Postives = 512/1281 (39.97%), Query Frame = 0

Query:  298 PTK-SKYGKKNVANLCSTLYHTVAVTTTGEVYGCGVNEDGQV-----------SPDKEDQ----------------YLWAPVLVERLLSQRVIQVSCGENHTACLTSSGAVLSFGNNEVGQLG--------------------HSAGATTTGEIVRSSRGSRRVEPRTVVGLGGKL--------------------------------IKQIACGYLFTVMLSSEGEVFSCGVG--GCVGGQDGKDRFQAERVDALMFVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLPPCPSVSPVPGEEELGGGGTVSPYRAPVLVSQLNGLEVCMLACGDAHTLALTKSGQVWSMGANSSGQLGTGASATNAEPSIHPSSSSWRAT--EVQGLQTAHVFMVAAGGEQSFAXXXXXXXXXXXXXXXXXXKTIIGTAVPTTAATPGLSLDESTLDLIRKFSVIKPATSFLTTAQLLTLIQQAASTHQPDFLERTLFDIFSSPSLLNGSF-LSVAHSSSLTAK------------------------------------------------SSTXRSCSTSTLSGLDVQGLESIYVAVMKLNHTPLLSRL----MHAFSTALDHLLPVAKSLYDPDSLRCLLAY--WQCPL----------NSNTSLSRDTVLKLCEAIVSLPGDGRVQLFAWARADYPPHLFATRLLRPLHSHLEYHLKVNFGKGRAVPMLAV-IMNYLYAANERIREDGREIDDLDEGGREEGKRLIPAEQFANAAVSALSVESLLHDLGTWKALKEENKNNINKNV-----FAFCRFPFLLSAEAKQRLLHGEAALGQQQAAQRDLMRQMFSG------GPVSPFLVLRVDRARLLESTLAQIGVCDAMMLKKQLKVSFVG----EEGVDEGGVKKEFFQLLCAQLFDFTYGMFRSTESGREVWFSPSHGPSFGSDDSQEYQLVGVLVGLAIYNSVLLDLHFPLVAYKKLLGLPVGFADLKELDAEMGEGLRKLLEWEEEGGREGGCGGGKVEDIFGLCFEVDWMEFGEVRRHELIEGGREIEVTAANRRDYVRLYVEWVLDGSIRKSFMDFKSGFDKVMGGSSISLLKPEELMLLVCGTPHMNLRELEAVARYEGGYDKSSNVIQWFWAVVHEMDLTSQRQLLMFVTGSAKAPIGGLGKLSLLIQRAGPESDHLPTAHTCFNVLLMPEYGSKEKLKERLMKAISECRGFGLQ 1414
            PTK  +   K++ ++ S+ ++++ +T  GEVY CG N+ GQ+           +P   D                 +   PV V  L   ++   S G+ H   +T  G + S+G +E GQLG                    HS+     G+ VR  R S       V+   G L                                I  + CG   T  LS +G VF+ G G  G +G  D   R   E V AL+                                                                                                G     G GT+     P  V  L    V   + G  H+L LT +  V+S G    GQLGT                S ++T  +V GL T  +  + AGG+ S                    + I G  V      P   ++                            +  A        +E    D+FSSP  L+  F L  A +                                                      ++   S   S    +D Q ++S+Y A++K+  T ++S L    +        HL   A    + ++   + A   W  PL            +  +    +  L +  ++LP   +  L  W  +  P   F  R++RPL  +L       F   R   M  V I++YL+ AN  +                     IP  +F N  +S     +L  +   +  LK  +++N             C+ PFLL+ EAK R+L GEA + +    Q   M  +  G      G V+ FL + V R+ +L+ +L QI V     LKK L+V+F+     EEG+DEGGV +EFFQLL A++F   YGMF   E  R  WF+ +       + + E+QLVG+++GLAIYN ++LD+HFPLV YKKLLG     ADL E    + +GL++LL++E           G VE+ F   F+V++  +G +++ +L+ GG  I VTA NR++YV+LY EW L  SI K F  F  GF  V GG ++SL + +EL LLVCG PH++  ELE  A+YE GY + S  + +FW  VH M +  +R+LL F TG  +AP+GGLG+L+L++QR GP++D LPT+HTCFN LL+PEY SKEKL ERL  AI   +GFGL+
Sbjct:   51 PTKVEQLQGKDIVHVASSAFNSIFLTDDGEVYTCGSNDSGQLGVKLIHQLKEDAPTARDDNHAAMSDAELKTSSWGFTSVPVRVMALDLYKIQHASSGQTHMLVVTEQGQLASWGASEFGQLGQGGEGLSHHQPRILKTCNNIHSSKGPQAGDHVRYIRSSSGACHNAVLDSYGHLHTFGQGTFGALGQGDEDNRDTPCLVKQLWPVGITMVTCGENHTCALSLDGRVFTWGRGKYGQLGHGDFGSRSCPELVKALLGSSIIQIACGGDHTLAVSNEGVLYSWGR--------------------------------------------------------------------GTWGQCGNGTLDNTCRPGRVQGLIDYRVLQASAGLRHSLVLTSTNDVYSFGDGEQGQLGTXXXXXXXXXXXXXXXXSCQSTPAKVLGLPTLPILFIVAGGDHSLVVLGEPAPGQRIPIGPESAR-IHGRGVVPVQPLPSGLIELCAAXXXXXXXXXXXXXXXXXXXXXXNTMPPAGLPGLVSCIE----DLFSSPGYLSSGFALPEAQNPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGHGTAAEFSSLMSITHNIDTQAVQSVYQAILKVYQTEVVSALGSSCLRLLQGIEQHLKWSALRAAEGNTAMSMTAQLEWLLPLLLILFLNPLNGEHLPVGTSLMTHLVDVYLTLPSRAQQLLALWL-SRLPIEQFGGRVVRPLQRYLSGLASQGFTSRRGKIMRGVQILDYLFDANHYLGSP------------------IPLSEFHNRTIS--ETANLKDEYLMFAELKNSSRSNYAVGAGMEAPTCLCQVPFLLTPEAKSRILQGEATVQKHHVMQNSAMAALMQGLHPAAIGSVA-FLDIHVRRSHILQDSLQQI-VSRPQDLKKPLRVTFISNGVPEEGLDEGGVSREFFQLLVAEIFRPDYGMFIYNEETRTFWFNAA-----SLESTVEFQLVGIVMGLAIYNGIILDVHFPLVVYKKLLGQHPTLADLAEAQPSLAKGLQQLLDFE-----------GDVENTFCRTFDVEYEYYGVMKKQDLLPGGSNIPVTALNRQEYVKLYTEWQLTSSIEKQFNAFAHGFHLVCGGLALSLFRYDELELLVCGLPHLDFHELEKNAKYE-GYTRDSPTVNFFWQAVHSMTMEQKRKLLAFTTGCDRAPVGGLGQLTLIVQRGGPDTDRLPTSHTCFNTLLLPEYSSKEKLNERLALAIENAQGFGLR 1218          

BLAST of NO04G01130 vs. NCBI_GenBank
Match: XP_005849888.1 (hypothetical protein CHLNCDRAFT_21324 [Chlorella variabilis] >EFN57786.1 hypothetical protein CHLNCDRAFT_21324 [Chlorella variabilis])

HSP 1 Score: 380.9 bits (977), Expect = 1.700e-101
Identity = 210/472 (44.49%), Postives = 287/472 (60.81%), Query Frame = 0

Query:  948 DLDEGGREEGKRLIPAEQFANAAVSALSVESLLHDLGTWKALKEENKNNINKNVFAFCRFPFLLSAEAKQRLLHGEAA--LGQQQAAQRDLMRQMFSGGPVSPFLVLRVDRARLLESTLAQIGVCDAMMLKKQLKVSF----VGEEGVDEGGVKKEFFQLLCAQLFDFTYGMFRSTESGREVWFSPSHGPSFGSDDSQEYQLVGVLVGLAIYNSVLLDLHFPLVAYKKLLGLPVGFADLKELDAEMGEGLRKLLEWEEEGGREGGCGGGKVEDIFGLCFEVDWMEFGEVRRHELIEGGREIEVTAANRRDYVRLYVEWVLDGSIRKSFMDFKSGFDKVMGGSSISLLKPEELMLLVCGTPHMNLRELEAVARYEGGYDKSSNVIQWFWAVVHEMDLTSQRQLLMFVTGSAKAPIGGLGKLSLLIQRAGPESDHLPTAHTCFNVLLMPEYGSKEKLKERLMKAISECRGFGLQ 1414
            D+     E+G  +IP  +F NA +S  +  +L  +   W+      +N     + + C+ P+LL+ EAK  ++HGEAA                         FL +RV R+ ++E  L Q+   +A  LKK L+V+F    V E   DEGGV KEFFQLL   LF   Y MF   E  R  WFSP+   S  ++D  EY LVG ++GLAIYN+V+LD+HFPL  YKKLLG+   FADLK    ++G GL++LL++E           G VE +F   F   +  +GE+R  EL  GG  I VTAANRR+YV LY  W+L+GS+ + F  F++GF +V GG +++L  P EL LLVCG PH++   L+AVA+YEGGY      ++WFW V+H + L  +R  LMF TGS +AP+GGLGKL LLIQRAGP+SD+LPT+HTCFN LL+PEY SK++L+ +L+ A+   +GFGLQ
Sbjct:    7 DILRQASEDGGGIIPYTEFHNAQLSEHA--NLQAEYVAWR------QNTSTTALCSICQMPYLLTPEAKSFIMHGEAAXXXXXXXXXXXXXXXXXXXXXXXXAFLQIRVRRSHVVEDALNQLAHLEAQDLKKPLRVTFITGGVPEPAQDEGGVTKEFFQLLTRDLFRPEYAMFTYNELTRTHWFSPA---SLEAED--EYALVGAVLGLAIYNAVILDVHFPLAVYKKLLGIRPTFADLKAAFPDLGRGLQQLLDFE-----------GDVEAVFCRSFTAQYEFYGEMREEELKPGGASIPVTAANRREYVDLYTAWLLEGSVGRQFGAFRAGFLRVCGGPALTLFTPAELELLVCGLPHLDFEALQAVAKYEGGYSAEHQEVRWFWEVLHSLSLEQKRAFLMFTTGSDRAPVGGLGKLPLLIQRAGPDSDNLPTSHTCFNSLLLPEYASKDRLRAKLLTAVENAQGFGLQ 454          

BLAST of NO04G01130 vs. NCBI_GenBank
Match: CBJ32015.1 (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 379.4 bits (973), Expect = 4.800e-101
Identity = 232/605 (38.35%), Postives = 324/605 (53.55%), Query Frame = 0

Query:  879 GRVQLFAWARADYPPHLFATRLLRPLHSHLEYHLKVNFGKGRAVPMLAVIMNYLYAANERIREDGREIDDLDEGGREEGKRLIPAEQFANAAVSALSVESLLHDLGTWKALKEENKNNINKNVFAFCRFPFLLSAEAKQRLLHGEAALGQQQAAQRDLMRQMFSGGPVSPFLVLRVDRARLLESTLAQ------------------------------------------IG----------VCDAMM----------------LKKQLKVSFVGEEGVDEGGVKKEFFQLLCAQLFDFTYGMF-RSTESGREVWFSPSHGPSFGSDDSQEYQLVGVLVGLAIYNSVLLDLHFPLVAYKKLLGLPVGFADLKELDAEMGEGLRKLLEWEEEGGREGGCGGGKVEDIFGLCFEVDWMEFGEVRRHELIEGGREIEVTAANRRDYVRLYVEWVLDGSIRKSFMDFKSGFDKVM-GGSSISLLKPEELMLLVCGTPHMNLRELEAVARYEGGYDKSSNVIQWFWAVVHEMDLTSQRQLLMFVTGSAKAPIGGLGKLSLLIQRAGPESDHLPTAHTCFNVLLMPEYGSKEKLKERLMKAISECRGFGLQ 1414
            G   L A A+ DY   LF++RL++P+  HL   LK    +    P  A+++ +L++ NE                  EG   +P E F +  +S +  ++++ DL TWK    +   ++    F  C  PFL+S + K+ LL  EA + Q +AAQ         G  ++P+LVL V+R  LL+ TLAQ                                          +G           C A                  L+K+LKV F GE+GVDEGGV KEFFQLL  QLFD ++GMF  + E GR +WF+        +D  +EY LVG+LVGLA+Y+ V+LD+  PLV +KK+LG  +   DL ++D  + +G  +L +++          GG  ED+FGL F V W + G  R H+L+EGG  I VT+ N+  YV LY +++L G++ +    FK GF +VM G +SISL + EEL +LV GT  ++  EL     Y+GGYD+    I  FW  V  M    QR+LLMFVTGS KAP+GGLGKL+  IQRAGP++DHLPTAHTCFN L++PEY  +EKL   L +AI+EC GFGL+
Sbjct:  641 GARPLVAHAQEDYSAELFSSRLVKPMVEHLSIWLKRVGQRRPYYPTAALLLKWLHSINEL-----------------EGTPKVPYEDFFSDYISNMRADNVVQDLLTWK----DQHPSVRIKRFFLCEHPFLMSTDLKKFLLKAEAQVEQDKAAQASGHYVPLVGVVINPYLVLTVNRTHLLQETLAQGTHSTQRTINSATAPAVHFYATLLAYAPSSPSSLYCIFPTLWLGGFALDPKPPPCCAAFPPHVPRPAKVSRLPDADLRKRLKVVFRGEDGVDEGGVTKEFFQLLTVQLFDQSFGMFVPAGEGGRVLWFNKD---CVWAD--EEYGLVGLLVGLAVYSGVILDVPLPLVVFKKVLGEQLSLEDLGDIDPGLLKGFHQLRDYD----------GGDDEDVFGLDFRVTWDDLGMERSHDLVEGGANIPVTSENKESYVELYAQFLLVGAVSRQMDHFKQGFLRVMSGAASISLFRAEELEVLVTGTSELDFEELAKATEYDGGYDEDHRTILAFWRAVGRMPAEEQRRLLMFVTGSKKAPLGGLGKLTFKIQRAGPDTDHLPTAHTCFNTLVLPEYSGEEKLTRLLKRAIAECEGFGLR 1209