NO01G00450, NO01G00450 (gene) Nannochloropsis oceanica | NanDeSyn: Nannochloropsis Design & Synthesis Initiative

Overview


Name	NO01G00450
Unique Name	NO01G00450
Type	gene
Organism	Nannochloropsis oceanica (N. oceanica IMET1)
Sequence length	978
Alignment location	chr1:122838..123815 -

Link to JBrowse

Properties


Property Name	Value
Description	4-methyl-5(B-hydroxyethyl)-thiazole monophosphate biosynthesis

Mutants

Expression

Analysis:

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Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
chr1	genome	chr1:122838..123815 -

Analyses

This gene is derived from or has results from the following analyses


Analysis Name	Date Performed
GSE178672	2023-12-15
PRJNA933693	2023-10-26
PRJNA943449	2023-07-19
PXD016054	2021-01-14
PXD016699	2021-01-08
GSE149904	2020-10-10
NoIMET1_WT_HS	2020-09-29
PXD010030	2020-03-16
GSE139615	2020-03-11
PRJNA241382	2020-03-11
BLASTP analysis of N. oceanica IMET1 genes	2019-07-11
InterPro analysis for N. oceanica IMET1 genes	2019-07-11
PXD008721	2019-04-30
PRJNA182180	2019-04-25
Gene prediction for N. oceanica IMET1	2017-10-24

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR029062	Class_I_gatase-like
IPR002818	ThiJ/PfpI

Homology

[Close]

BLAST of NO01G00450 vs. NCBI_GenBank
Match: XP_005855953.1 (dj-1 family protein [Nannochloropsis gaditana CCMP526] >EKU20396.1 dj-1 family protein [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 270.4 bits (690), Expect = 5.800e-69
Identity = 143/201 (71.14%), Postives = 164/201 (81.59%), Query Frame = 0

Query:   58 VLVPVADGSEEIESVTIIDVLVRAGATVTVASVMPSSXXXXXXXSPVNKHQVTCSRGVKLVADKGIDDCLEQEWDLIACPGGLPGAEYLRDSSSLLTLLTKQEASKKYIAAICASPAMVLQTHGFLKGRSATCFPAERFTTTLEKYQGKDGGAVVVDGHVVTSRGPGTTMVFALKLVELLFGMDKAKEVAGGLLVTYPEEG 259
            VLVPVADGSEEIESVTIIDVLVRAGA VTVASVMPS+       S     QVTCSRGVKLVADKGIDDCL +EWDLIACPGGLPGAE+LRDS +LLTLL +Q+ +++ +AAICASPA+VL THGFL G+ ATCFPAERF ++L +Y+  + G VV DGHVVTSRGPGTT+ FAL LV+ L G  KA+EVA GLLV YP +G
Sbjct:    8 VLVPVADGSEEIESVTIIDVLVRAGAAVTVASVMPSASTATLPRS----WQVTCSRGVKLVADKGIDDCLGEEWDLIACPGGLPGAEHLRDSPALLTLLQRQDKAQRLVAAICASPAVVLHTHGFLTGKRATCFPAERFVSSLARYEDGEEG-VVEDGHVVTSRGPGTTLRFALTLVDRLCGRAKAEEVAKGLLVDYPGKG 203

[Close]

BLAST of NO01G00450 vs. NCBI_GenBank
Match: EWM28974.1 (4-methyl-5(b-hydroxyethyl)-thiazole monophosphate biosynthesis [Nannochloropsis gaditana])

HSP 1 Score: 270.4 bits (690), Expect = 5.800e-69
Identity = 143/201 (71.14%), Postives = 164/201 (81.59%), Query Frame = 0

Query:   58 VLVPVADGSEEIESVTIIDVLVRAGATVTVASVMPSSXXXXXXXSPVNKHQVTCSRGVKLVADKGIDDCLEQEWDLIACPGGLPGAEYLRDSSSLLTLLTKQEASKKYIAAICASPAMVLQTHGFLKGRSATCFPAERFTTTLEKYQGKDGGAVVVDGHVVTSRGPGTTMVFALKLVELLFGMDKAKEVAGGLLVTYPEEG 259
            VLVPVADGSEEIESVTIIDVLVRAGA VTVASVMPS+       S     QVTCSRGVKLVADKGIDDCL +EWDLIACPGGLPGAE+LRDS +LLTLL +Q+ +++ +AAICASPA+VL THGFL G+ ATCFPAERF ++L +Y+  + G VV DGHVVTSRGPGTT+ FAL LV+ L G  KA+EVA GLLV YP +G
Sbjct:   63 VLVPVADGSEEIESVTIIDVLVRAGAAVTVASVMPSASTATLPSS----WQVTCSRGVKLVADKGIDDCLGEEWDLIACPGGLPGAEHLRDSPALLTLLQRQDKAQRLVAAICASPAVVLHTHGFLTGKRATCFPAERFVSSLARYEDGEEG-VVEDGHVVTSRGPGTTLRFALTLVDRLCGRAKAEEVAKGLLVDYPGKG 258

[Close]

BLAST of NO01G00450 vs. NCBI_GenBank
Match: EWM28975.1 (4-methyl-5(b-hydroxyethyl)-thiazole monophosphate biosynthesis [Nannochloropsis gaditana])

HSP 1 Score: 270.4 bits (690), Expect = 5.800e-69
Identity = 143/201 (71.14%), Postives = 164/201 (81.59%), Query Frame = 0

Query:   58 VLVPVADGSEEIESVTIIDVLVRAGATVTVASVMPSSXXXXXXXSPVNKHQVTCSRGVKLVADKGIDDCLEQEWDLIACPGGLPGAEYLRDSSSLLTLLTKQEASKKYIAAICASPAMVLQTHGFLKGRSATCFPAERFTTTLEKYQGKDGGAVVVDGHVVTSRGPGTTMVFALKLVELLFGMDKAKEVAGGLLVTYPEEG 259
            VLVPVADGSEEIESVTIIDVLVRAGA VTVASVMPS+       S     QVTCSRGVKLVADKGIDDCL +EWDLIACPGGLPGAE+LRDS +LLTLL +Q+ +++ +AAICASPA+VL THGFL G+ ATCFPAERF ++L +Y+  + G VV DGHVVTSRGPGTT+ FAL LV+ L G  KA+EVA GLLV YP +G
Sbjct:   91 VLVPVADGSEEIESVTIIDVLVRAGAAVTVASVMPSASTATLPSS----WQVTCSRGVKLVADKGIDDCLGEEWDLIACPGGLPGAEHLRDSPALLTLLQRQDKAQRLVAAICASPAVVLHTHGFLTGKRATCFPAERFVSSLARYEDGEEG-VVEDGHVVTSRGPGTTLRFALTLVDRLCGRAKAEEVAKGLLVDYPGKG 286

[Close]

BLAST of NO01G00450 vs. NCBI_GenBank
Match: PQP35028.1 (DJ-1 family protein [Desulfobacteraceae bacterium SEEP-SAG9])

HSP 1 Score: 195.7 bits (496), Expect = 1.800e-46
Identity = 111/197 (56.35%), Postives = 134/197 (68.02%), Query Frame = 0

Query:   58 VLVPVADGSEEIESVTIIDVLVRAGATVTVASVMPSSXXXXXXXSPVNKHQVTCSRGVKLVADKGIDDCLEQEWDLIACPGGLPGAEYLRDSSSLLTLLTKQEASKKYIAAICASPAMVLQTHGFLKGRSATCFPAERFTTTLEKYQGKDGGAVVVDGHVVTSRGPGTTMVFALKLVELLFGMDKAKEVAGGLLVTY 255
            VLVP+ADG+EEIE+V IIDVL RAGA VTVAS              VN  QVT SRGVKLVADK I DC  + +DLIA PGG+PGAE+LRDS+ L  +L +Q  S +    ICA+PA+VLQ HGFLKGR ATC P   F   LE     +   VVVDG  +TSRGPGT + FALKLVE+LFG +K +E++  ++V Y
Sbjct:    5 VLVPIADGTEEIEAVCIIDVLRRAGADVTVAS--------------VNSLQVTASRGVKLVADKLISDCTNETYDLIALPGGIPGAEHLRDSAELEQMLREQYESNRLYGGICAAPAVVLQNHGFLKGRQATCHPV--FVHQLENTDLVE-TRVVVDGPCITSRGPGTALEFALKLVEMLFGKEKVEEISQPMMVRY 184

[Close]

BLAST of NO01G00450 vs. NCBI_GenBank
Match: XP_002287916.1 (predicted protein [Thalassiosira pseudonana CCMP1335] >EED95359.1 predicted protein [Thalassiosira pseudonana CCMP1335])

HSP 1 Score: 189.5 bits (480), Expect = 1.300e-44
Identity = 106/197 (53.81%), Postives = 136/197 (69.04%), Query Frame = 0

Query:   58 VLVPVADGSEEIESVTIIDVLVRAGATVTVASVMPSSXXXXXXXSPVNKHQVTC--SRGVKLVADKGIDDCLEQEWDLIACPGGLPGAEYLRDSSSLLTLLTKQEASKKYIAAICASPAMVLQTHGFLKGRSATCFPAERFTTTLEKYQGKDGGAVVVDGHVVTSRGPGTTMVFALKLVELLFGMDKAKEVAGGLLV 253
            VLVP+AD SEEIE+  I D LVR GA V VASV P               ++ C  SRGVK+VAD  ID+ + QEWDL+A PGG+PGAE+LRDS++L+++L KQ+AS K   AICASPA+VL T G + G  ATCFPA+   + +     +D   VVV G+VVTS+GPGT + F +KL ELL+G ++AKEVAGGLL+
Sbjct:    5 VLVPIADDSEEIETTCITDTLVRFGAEVVVASVKPGG-------------ELVCKMSRGVKMVADISIDEAVGQEWDLVALPGGMPGAEHLRDSATLISILEKQKASGKLYGAICASPAVVLATKGLI-GEGATCFPADGLRSKMASPVDED---VVVQGNVVTSKGPGTALKFGVKLGELLYGEERAKEVAGGLLI 184

[Close]

BLAST of NO01G00450 vs. NCBI_GenBank
Match: CBJ30585.1 (4-methyl-5(b-hydroxyethyl)-thiazole monophosphate biosynthesis protein, putative [Ectocarpus siliculosus])

HSP 1 Score: 188.7 bits (478), Expect = 2.200e-44
Identity = 101/194 (52.06%), Postives = 135/194 (69.59%), Query Frame = 0

Query:   58 VLVPVADGSEEIESVTIIDVLVRAGATVTVASVMPSSXXXXXXXSPVNKHQVTCSRGVKLVADKGIDDCLEQEWDLIACPGGLPGAEYLRDSSSLLTLLTKQEASKKYIAAICASPAMVLQTHGFLKGRSATCFPAERFTTTLEKYQGKDGGAVVVDGHVVTSRGPGTTMVFALKLVELLFGMDKAKEVAGGLL 252
            VLVPVADGSEEIESVTIID LVRAGA VTVASV P             + +VTCSRGVK+ AD  I +C  ++WD + CPGG+PGA  L+++ +L  +L KQ +  + + AICA+PA+VL +HG L+G+ ATC+PA  F + + +   +    VVVD +++TS+GP T+M FAL+LV  LFG +K+ EVA GLL
Sbjct:   59 VLVPVADGSEEIESVTIIDTLVRAGALVTVASVGP-------------EVEVTCSRGVKIKADCKIAECEARDWDAVVCPGGMPGAVSLKENETLEAILRKQNSEGRIVGAICAAPAVVLASHGLLEGKQATCYPASVFQSKIPQLVSE---KVVVDQNLITSQGPATSMAFALQLVGSLFGQEKSDEVAKGLL 236

[Close]

BLAST of NO01G00450 vs. NCBI_GenBank
Match: KPJ85360.1 (dihydroxyacetone kinase [Spirochaetes bacterium DG_61])

HSP 1 Score: 187.2 bits (474), Expect = 6.500e-44
Identity = 107/195 (54.87%), Postives = 134/195 (68.72%), Query Frame = 0

Query:   58 VLVPVADGSEEIESVTIIDVLVRAGATVTVASVMPSSXXXXXXXSPVNKHQVTCSRGVKLVADKGIDDCLEQEWDLIACPGGLPGAEYLRDSSSLLTLLTKQEASKKYIAAICASPAMVLQTHGFLKGRSATCFPAERFTTTLEKYQGKDGGAVVVDGHVVTSRGPGTTMVFALKLVELLFGMDKAKEVAGGLLV 253
            VL+P+ADGSEEIE+V IIDVL RAGA+VTVAS              V+  QV  SRGVKL+AD  I +C+++ +DLIA PGG+PGAE++RDS  L  +L +Q+   +   AICASPA+VL  HG L G+ ATC P   F+  LE  +  D   VVVDG+ VTSRGPGT + FALKLVELLFG +KA EVA  ++V
Sbjct:    5 VLIPIADGSEEIEAVCIIDVLRRAGASVTVAS--------------VDGLQVIASRGVKLIADNLISECVDRTFDLIALPGGMPGAEHMRDSRELTEMLKQQQKEGRLYGAICASPAVVLMHHGLLSGKRATCHP--DFSDRLENREEVD-SRVVVDGNCVTSRGPGTAIEFALKLVELLFGSEKAAEVAKPMIV 182

[Close]

BLAST of NO01G00450 vs. NCBI_GenBank
Match: XP_005709203.1 (4-methyl-5(b-hydroxyethyl)-thiazole monophosphate biosynthesis [Galdieria sulphuraria] >EME32683.1 4-methyl-5(b-hydroxyethyl)-thiazole monophosphate biosynthesis [Galdieria sulphuraria])

HSP 1 Score: 184.9 bits (468), Expect = 3.200e-43
Identity = 103/202 (50.99%), Postives = 139/202 (68.81%), Query Frame = 0

Query:   58 VLVPVADGSEEIESVTIIDVLVRAGATVTVASVMPSSXXXXXXXSPVNKHQVTCSRGVKLVADKGIDDCLEQEWDLIACPGGLPGAEYLRDSSSLLTLLTKQEASKKYIAAICASPAMVLQTHGFLKGR-SATCFPAERFTTTLEKYQGKDGGAVVVDGHVVTSRGPGTTMVFALKLVELLFGMDKAKEVAGGLLVTYPEEG 259
            VLVP+A+G+EEIE+VTI D LVRAGA VT+ASV              N+ Q+T SRGV++VADK I DC  +++DLIA PGG  GAE L     L+TLL +Q+ S K+I AICA+PA+VL  +GFL+    ATC+PA++F + L+     +   VVVDG  +TS+GPGT + F+L LVE L+G  KA+E+A  +L+T PE+G
Sbjct:   68 VLVPIANGTEEIEAVTIADTLVRAGAQVTIASV-------------ENQLQITASRGVRIVADKLISDCTNEQYDLIAIPGGAKGAEKLGSCEELITLLRQQQQSGKFIGAICAAPALVLAENGFLEDSIRATCYPADQFLSKLKNPVDDEDCPVVVDGQFITSQGPGTALHFSLTLVEKLYGRQKAEELAALMLLT-PEDG 255

[Close]

BLAST of NO01G00450 vs. NCBI_GenBank
Match: GAK61017.1 (hypothetical protein U27_00915 [Candidatus Vecturithrix granuli])

HSP 1 Score: 183.3 bits (464), Expect = 9.300e-43
Identity = 102/195 (52.31%), Postives = 133/195 (68.21%), Query Frame = 0

Query:   58 VLVPVADGSEEIESVTIIDVLVRAGATVTVASVMPSSXXXXXXXSPVNKHQVTCSRGVKLVADKGIDDCLEQEWDLIACPGGLPGAEYLRDSSSLLTLLTKQEASKKYIAAICASPAMVLQTHGFLKGRSATCFPAERFTTTLEKYQGKDGGAVVVDGHVVTSRGPGTTMVFALKLVELLFGMDKAKEVAGGLLV 253
            VLVP+ADG+EEIE+V  ID L R+G  VTVASVM             +  ++T SRGVKLVADK I +C+ + +D+IA PGG+PGAE+LRDS  L+ +L +Q    +  AAICASP +VLQ HG L+G+ ATC P+  F   LE     +   VVVDG+ VTSR PGT + FALKL+ELLFG + A++VAG ++V
Sbjct:    5 VLVPIADGTEEIEAVATIDTLRRSGVQVTVASVM-------------DHLEITASRGVKLVADKLISECVHETYDVIALPGGIPGAEHLRDSEELIHMLRQQHQEGRIYAAICASPVVVLQHHGLLQGKKATCHPS--FADKLEDRDAVE-QRVVVDGNCVTSRAPGTAIEFALKLIELLFGAEHAQKVAGPMVV 183

[Close]

BLAST of NO01G00450 vs. NCBI_GenBank
Match: OHB86084.1 (dihydroxyacetone kinase [Planctomycetes bacterium RIFCSPHIGHO2_02_FULL_40_12])

HSP 1 Score: 183.3 bits (464), Expect = 9.300e-43
Identity = 104/197 (52.79%), Postives = 130/197 (65.99%), Query Frame = 0

Query:   58 VLVPVADGSEEIESVTIIDVLVRAGATVTVASVMPSSXXXXXXXSPVNKHQVTCSRGVKLVADKGIDDCLEQEWDLIACPGGLPGAEYLRDSSSLLTLLTKQEASKKYIAAICASPAMVLQTHGFLKGRSATCFPAERFTTTLEKYQGKDGGAVVVDGHVVTSRGPGTTMVFALKLVELLFGMDKAKEVAGGLLVTY 255
            VLVPVADGSEEIE+  I+DVL RA A VTVAS              VN  Q+T SRG+KLVAD+ I +C++  +DLI  PGG+PGAE+LRDS  L+ +L +Q+   +  AAICASPA+VL  HG L  R AT +P   F    E  +  D   VVVDG+ +TSR PGT M FALKLVELL+G  KA+E+A  +LV +
Sbjct:    5 VLVPVADGSEEIETACIVDVLRRAEADVTVAS--------------VNSLQITASRGMKLVADQLISECVQNTYDLIVLPGGMPGAEHLRDSKELVEMLKRQQNDGRLYAAICASPAVVLHHHGLLAQRKATAYPG--FAEQFENTEAID-SRVVVDGNCITSRAPGTAMEFALKLVELLYGEQKAREIAQSMLVDW 184

The following BLAST results are available for this feature:

BLAST of NO01G00450 vs. NCBI_GenBank

BLAST of NO01G00450 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes)
Total hits: 20


Match Name	E-value	Identity	Description
XP_005855953.1	5.800e-69	71.14	dj-1 family protein [Nannochloropsis gaditana CCMP...	[more]
EWM28974.1	5.800e-69	71.14	4-methyl-5(b-hydroxyethyl)-thiazole monophosphate ...	[more]
EWM28975.1	5.800e-69	71.14	4-methyl-5(b-hydroxyethyl)-thiazole monophosphate ...	[more]
PQP35028.1	1.800e-46	56.35	DJ-1 family protein [Desulfobacteraceae bacterium ...	[more]
XP_002287916.1	1.300e-44	53.81	predicted protein [Thalassiosira pseudonana CCMP13...	[more]
CBJ30585.1	2.200e-44	52.06	4-methyl-5(b-hydroxyethyl)-thiazole monophosphate ...	[more]
KPJ85360.1	6.500e-44	54.87	dihydroxyacetone kinase [Spirochaetes bacterium DG...	[more]
XP_005709203.1	3.200e-43	50.99	4-methyl-5(b-hydroxyethyl)-thiazole monophosphate ...	[more]
GAK61017.1	9.300e-43	52.31	hypothetical protein U27_00915 [Candidatus Vecturi...	[more]
OHB86084.1	9.300e-43	52.79	dihydroxyacetone kinase [Planctomycetes bacterium ...	[more]

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Relationships

This gene is member of the following syntenic_region feature(s):


Feature Name	Unique Name	Species	Type
nonsL006	nonsL006	Nannochloropsis oceanica (N. oceanica IMET1)	syntenic_region
ncniR047	ncniR047	Nannochloropsis oceanica (N. oceanica IMET1)	syntenic_region
ngnoR091	ngnoR091	Nannochloropsis oceanica (N. oceanica IMET1)	syntenic_region

This gene is orthologous to the following gene feature(s):


Feature Name	Unique Name	Species	Type
NSK008342	NSK008342	Nannochloropsis salina (N. salina CCMP1776)	gene

The following polypeptide feature(s) derives from this gene:


Feature Name	Unique Name	Species	Type
NO01G00450.1	NO01G00450.1-protein	Nannochloropsis oceanica (N. oceanica IMET1)	polypeptide

The following gene feature(s) are orthologous to this gene:


Feature Name	Unique Name	Species	Type
jgi.p\|Nanoce1779_2\|575933	gene_22	Nannochloropsis oceanica (N. oceanica CCMP1779)	gene
thiJ	gene1640	Nannochloropsis gaditana (N. gaditana B-31)	gene

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Species	Type
NO01G00450.1	NO01G00450.1	Nannochloropsis oceanica (N. oceanica IMET1)	mRNA

Sequences

The following sequences are available for this feature:

gene sequence
protein sequence

gene sequence

>NO01G00450 ID=NO01G00450|Name=NO01G00450|organism=Nannochloropsis oceanica|type=gene|length=978bp
AGGCAGACCAGCTGTAAGTGCACGTATTATTCGCAGAGCTTGGAATGGCA
GCAGgtaaggtcaacctcctcaccccacataacgctttcccccggtcgct
ccctcacttcctttgcttccctttcccgcacgctctcattcagTTTTCTT
CTCCATTCCAACCACTGATACATGACGACATTTTGCTTCTCTGCCCTCTT
CATCACCATGCTCTGTCTCTCCACCTTTCTTCCACGTACCACCACTGCCT
TTCTCCTTCGTTCCTCCTCCTCGTCATCCCTCCGCCCGACCTTTCACCGC
CGCTTCTCCTCCTCTTCAACCCCTGCAGCAACCCAGAAGAAAGTGCTCGT
CCCCGTTGCGGACGGCTCGGAAGAGATCGAATCTGTGACCATCATCGATG
TTCTCGTGCGCGCTGGCGCGACCGTCACCGTCGCCTCCGTCATGCCCTCT
TCCGCGTCCCCTTCCTCCTCCTCCTCCCCTGTCAACAAGCACCAGGTGAC
CTGCTCCCGCGGTGTAAAGCTCGTGGCCGACAAGGGCATCGACGACTGCC
TTGAGCAGGAGTGGGACCTCATTGCTTGCCCCGGCGGGCTTCCGGGTGCG
GAATATCTGCGTGATTCTTCCTCTCTCCTCACCCTCCTGACGAAGCAAGA
GGCTTCCAAAAAATACATCGCTGCTATATGTGCTTCGCCTGCCATGGTCT
TACAGACCCACGGGTTCTTAAAAGGCAGGTCCGCCACGTGTTTCCCCGCG
GAGCGCTTCACGACGACGCTGGAGAAGTACCAAGGGAAGGACGGAGGGGC
GGTGGTGGTCGATGGGCATGTGGTGACAAGTCGGGGCCCGGGGACGACGA
TGGTCTTTGCCTTAAAGTTGGTCGAGCTTTTGTTTGGCATGGATAAGGCA
AAGGAGGTGGCAGGGGGGCTGCTGGTTACCTACCCGGAGGAAGGGTAGAG
AGGGAGGGGGGAAGGAGGGAGGGAGGGA

protein sequence of NO01G00450.1

>NO01G00450.1-protein ID=NO01G00450.1-protein|Name=NO01G00450.1|organism=Nannochloropsis oceanica|type=polypeptide|length=259bp
MTTFCFSALFITMLCLSTFLPRTTTAFLLRSSSSSSLRPTFHRRFSSSST
PAATQKKVLVPVADGSEEIESVTIIDVLVRAGATVTVASVMPSSASPSSS
SSPVNKHQVTCSRGVKLVADKGIDDCLEQEWDLIACPGGLPGAEYLRDSS
SLLTLLTKQEASKKYIAAICASPAMVLQTHGFLKGRSATCFPAERFTTTL
EKYQGKDGGAVVVDGHVVTSRGPGTTMVFALKLVELLFGMDKAKEVAGGL
LVTYPEEG*

Synonyms

Publications