NO24G00040, NO24G00040 (gene) Nannochloropsis oceanica

Overview
NameNO24G00040
Unique NameNO24G00040
Typegene
OrganismNannochloropsis oceanica (N. oceanica IMET1)
Sequence length2574
Alignment locationchr24:9946..12519 -

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Properties
Property NameValue
DescriptionDihydrofolate reductase
Mutants
Expression

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
chr24genomechr24:9946..12519 -
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
PRJNA7699582024-08-13
GSE1786722023-12-15
PRJNA9336932023-10-26
PRJNA9434492023-07-19
GSE1499042020-10-10
NoIMET1_WT_HS2020-09-29
GSE1396152020-03-11
PRJNA2413822020-03-11
BLASTP analysis of N. oceanica IMET1 genes2019-07-11
InterPro analysis for N. oceanica IMET1 genes2019-07-11
GO annotation for IMET1v2 genes2019-07-10
PRJNA1821802019-04-25
Gene prediction for N. oceanica IMET12017-10-24
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0050661NADP binding
GO:0004146dihydrofolate reductase activity
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0006545glycine biosynthetic process
GO:0055114oxidation-reduction process
GO:0046654tetrahydrofolate biosynthetic process
GO:0009987cellular process
GO:0009058biosynthetic process
Vocabulary: INTERPRO
TermDefinition
IPR024072DHFR-like_dom
IPR012259DHFR
IPR001796DHFR_dom
Homology
[Close]
BLAST of NO24G00040 vs. NCBI_GenBank
Match: EWM20443.1 (dihydrofolate reductase, partial [Nannochloropsis gaditana])

HSP 1 Score: 110.9 bits (276), Expect = 4.700e-21
Identity = 46/50 (92.00%), Postives = 50/50 (100.00%), Query Frame = 0 
Query:   74 DDVRLIACVAPPNLTIGKGGQLPWDLPEDRKYFFHCTQGHVLVLGRRSYE 124
            DDVR+IACVAPPNLTIGKGGQLPWDLPEDR+YFFHCT+GH+LVLGRRSYE
Sbjct:  127 DDVRMIACVAPPNLTIGKGGQLPWDLPEDRQYFFHCTRGHILVLGRRSYE 176
[Close]
BLAST of NO24G00040 vs. NCBI_GenBank
Match: WP_087022642.1 (hypothetical protein [Butyricicoccus porcorum] >OUM19356.1 hypothetical protein CBW42_13590 [Butyricicoccus porcorum])

HSP 1 Score: 88.2 bits (217), Expect = 3.300e-14
Identity = 45/87 (51.72%), Postives = 59/87 (67.82%), Query Frame = 0 
Query:   76 VRLIACVAPPNLTIGKGGQLPWDLPEDRKYFFHCTQGHVLVLGRRSYEEAGHALPGRETVVVSRTLS-SLPDALVASSLTEALALAG 162
            + LIA  A  N  IGK G++PWD+P ++  F   T GHV+V+GR++YEE G  LPGRET+VVSRT   + P     S+L +ALALAG
Sbjct:    2 IALIAAYA-ENRVIGKDGRIPWDIPAEKHRFRDLTMGHVMVMGRKTYEEIGRPLPGRETIVVSRTKKFNEPHCTTVSNLPQALALAG 87
[Close]
BLAST of NO24G00040 vs. NCBI_GenBank
Match: WP_051456716.1 (dihydrofolate reductase [Ruminococcus flavefaciens] >EWM52290.1 hypothetical protein RF007C_13130 [Ruminococcus flavefaciens 007c])

HSP 1 Score: 85.5 bits (210), Expect = 2.100e-13
Identity = 51/111 (45.95%), Postives = 69/111 (62.16%), Query Frame = 0 
Query:   76 VRLIACVAPPNLTIGKGGQLPWDLPEDRKYFFHCTQGHVLVLGRRSYEEAGHALPGRETVVVSRTLSSLPDALV-ASSLTEALALAGK-IKRRKGREGMITWIGGEGMERE 185
            + +IA VA  N  IG GG++PW +P+D  YF   T GH++++GRRS+EE G  LP R  +VVSR+ S     L+ A SL++A+ LA K IK  K R   I   GG G+ RE
Sbjct:    2 INIIAAVA-ANGVIGSGGKIPWHIPQDMNYFRRITSGHIIIMGRRSFEEIGRPLPDRLNIVVSRSKSFRSSGLITAKSLSQAVELAEKEIKNGKLRR-EIFLCGGAGIYRE 110
[Close]
BLAST of NO24G00040 vs. NCBI_GenBank
Match: WP_091757925.1 (dihydrofolate reductase [Phycicoccus cremeus] >SES13283.1 dihydrofolate reductase [Phycicoccus cremeus])

HSP 1 Score: 85.5 bits (210), Expect = 2.100e-13
Identity = 40/77 (51.95%), Postives = 52/77 (67.53%), Query Frame = 0 
Query:   86 NLTIGKGGQLPWDLPEDRKYFFHCTQGHVLVLGRRSYEEAGHALPGRETVVVSRTLS-SLPDALVASSLTEALALAG 162
            N  IG+GG +PW LPED K+F H T GH +V+GR+++E  G  LPGR T+V++R      PD   A SL EAL+LAG
Sbjct:   13 NRVIGRGGGMPWHLPEDLKHFKHTTMGHPMVMGRKTFESIGRVLPGRRTIVITRQADWHHPDVEAAHSLPEALSLAG 89
[Close]
BLAST of NO24G00040 vs. NCBI_GenBank
Match: CCZ90444.1 (dihydrofolate reductase [Clostridium sp. CAG:167])

HSP 1 Score: 84.7 bits (208), Expect = 3.600e-13
Identity = 42/86 (48.84%), Postives = 59/86 (68.60%), Query Frame = 0 
Query:   76 VRLIACVAPPNLTIGKGGQLPWDLPEDRKYFFHCTQGHVLVLGRRSYEEAGHALPGRETVVVSRTLS-SLPDALVASSLTEALALA 161
            + LIA + P  + IG+ G++PW++P ++K F   T GH +V+GRR+YEE G  LPGR+T+VVS T +   P    A SLTEALAL+
Sbjct:    2 IALIAAMTPDRV-IGREGRIPWNIPGEQKRFKELTMGHTIVMGRRTYEEIGRPLPGRQTIVVSTTKNFDQPGCRTAGSLTEALALS 86
[Close]
BLAST of NO24G00040 vs. NCBI_GenBank
Match: WP_051007810.1 (hypothetical protein [Ruminococcus flavefaciens])

HSP 1 Score: 83.6 bits (205), Expect = 8.100e-13
Identity = 57/129 (44.19%), Postives = 77/129 (59.69%), Query Frame = 0 
Query:   76 VRLIACVAPPNLTIGKGGQLPWDLPEDRKYFFHCTQGHVLVLGRRSYEEAGHALPGRETVVVSRTLSSLPDALV-ASSLTEALALAGK-IKRRKGREGMITWIGGEGMEREEGGREGQELKAREAGRGL 203
            + +IA VA  N  IG GG++PW +P+D  YF   T GH++++GRRS+EE G  LP R  +VVSR+ S     L+ A SL++A+ LA K IK  K R   I   GG G+ R    R GQ L    AG+G+
Sbjct:    2 INIIAAVA-ANGVIGSGGKIPWHIPQDMNYFRRITSGHIIIMGRRSFEEIGRPLPDRLNIVVSRSKSFRSSGLITAKSLSQAVELAEKEIKNGKLRR-EIFLCGGAGISRRARHR-GQTL-PYGAGQGV 126
[Close]
BLAST of NO24G00040 vs. NCBI_GenBank
Match: KHO53723.1 (Dihydrofolate reductase [archaeon GW2011_AR11])

HSP 1 Score: 83.2 bits (204), Expect = 1.100e-12
Identity = 42/109 (38.53%), Postives = 62/109 (56.88%), Query Frame = 0 
Query:   76 VRLIACVAPPNLTIGKGGQLPWDLPEDRKYFFHCTQGHVLVLGRRSYEEAGHALPGRETVVVSRTLSSLPDALVASSLTEALALAGKIKRRKGREGMITWIGGEGMERE 185
            + ++  +      IGK G+LPWD+PE+  +F   T+G  +++GR++YE  GH LPGR  +VVSR    +P   VA S+ EA+ +A K  R       I  IGG  + RE
Sbjct:    2 IAIVVAMTKKGKVIGKDGRLPWDIPEELSHFRKLTKGGAIIMGRKTYESIGHPLPGRPNIVVSRNAGKVPGCEVAGSVPEAVEMAKKHHRD------IFIIGGAQIARE 104
[Close]
BLAST of NO24G00040 vs. NCBI_GenBank
Match: OGA14942.1 (hypothetical protein A3I63_01025 [Betaproteobacteria bacterium RIFCSPLOWO2_02_FULL_66_14])

HSP 1 Score: 83.2 bits (204), Expect = 1.100e-12
Identity = 47/87 (54.02%), Postives = 58/87 (66.67%), Query Frame = 0 
Query:   78 LIACVAPPNLTIGKGGQLPWDLPEDRKYFFHCTQGHVLVLGRRSYEEAGHALPGRETVVVSRTLSSL-PDALVASSLTEAL-ALAGK 163
            LIA VA  N  IG GG LPW LPED KYF   TQGH +++GR+++E  G ALPGR  +VV+R    + P+A VA SL  AL A AG+
Sbjct:   12 LIAAVA-ANGVIGSGGALPWRLPEDLKYFKRLTQGHTVIMGRKTWESIGRALPGRRNLVVTRQTGYVAPEASVAPSLEAALEACAGE 97
[Close]
BLAST of NO24G00040 vs. NCBI_GenBank
Match: PHX95124.1 (dihydrofolate reductase [Pedosphaera sp.])

HSP 1 Score: 83.2 bits (204), Expect = 1.100e-12
Identity = 43/87 (49.43%), Postives = 57/87 (65.52%), Query Frame = 0 
Query:   75 DVRLIACVAPPNLTIGKGGQLPWDLPEDRKYFFHCTQGHVLVLGRRSYEEAGHALPGRETVVVSRTLSSLPDALVASSLTEALALAG 162
            D + IA ++  N  IG+G ++PW LPED K+F   T GHVLV+GR+++E  G  LPGRET+V+SR   S P     +SL  A ALAG
Sbjct:    3 DFKAIAAMS-LNRVIGEGSKIPWHLPEDFKWFKATTLGHVLVMGRKTFESIGRPLPGRETIVLSRAGWSHPGVKTVASLHAAAALAG 88
[Close]
BLAST of NO24G00040 vs. NCBI_GenBank
Match: WP_009983674.1 (dihydrofolate reductase [Ruminococcus flavefaciens])

HSP 1 Score: 82.4 bits (202), Expect = 1.800e-12
Identity = 47/107 (43.93%), Postives = 62/107 (57.94%), Query Frame = 0 
Query:   79 IACVAPPNLTIGKGGQLPWDLPEDRKYFFHCTQGHVLVLGRRSYEEAGHALPGRETVVVSRTLSSLPD-ALVASSLTEALALAGKIKRRKGREGMITWIGGEGMERE 185
            I   A  N  IG GG++PWD+PED  YF   T G  +++GRR+YE+ G  LPGR  VVVS  + + P+   +A SL EA+ +AG      GRE  I   GG  + RE
Sbjct:    4 IIAAAAKNGVIGAGGKIPWDIPEDMAYFRRITYGGAVIMGRRTYEDIGRPLPGRFNVVVSAKMQTAPEGTALAHSLAEAVEIAG----NSGREN-IFLCGGAAVYRE 105
The following BLAST results are available for this feature:
BLAST of NO24G00040 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes)
Total hits: 20
Match NameE-valueIdentityDescription
EWM20443.14.700e-2192.00dihydrofolate reductase, partial [Nannochloropsis ... [more]
WP_087022642.13.300e-1451.72hypothetical protein [Butyricicoccus porcorum] >OU... [more]
WP_051456716.12.100e-1345.95dihydrofolate reductase [Ruminococcus flavefaciens... [more]
WP_091757925.12.100e-1351.95dihydrofolate reductase [Phycicoccus cremeus] >SES... [more]
CCZ90444.13.600e-1348.84dihydrofolate reductase [Clostridium sp. CAG:167][more]
WP_051007810.18.100e-1344.19hypothetical protein [Ruminococcus flavefaciens][more]
KHO53723.11.100e-1238.53Dihydrofolate reductase [archaeon GW2011_AR11][more]
OGA14942.11.100e-1254.02hypothetical protein A3I63_01025 [Betaproteobacter... [more]
PHX95124.11.100e-1249.43dihydrofolate reductase [Pedosphaera sp.][more]
WP_009983674.11.800e-1243.93dihydrofolate reductase [Ruminococcus flavefaciens... [more]

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Relationships

This gene is member of the following syntenic_region feature(s):

Feature NameUnique NameSpeciesType
ncniR013ncniR013Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ngnoR114ngnoR114Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region


The following polypeptide feature(s) derives from this gene:

Feature NameUnique NameSpeciesType
NO24G00040.3NO24G00040.3-proteinNannochloropsis oceanica (N. oceanica IMET1)polypeptide
NO24G00040.2NO24G00040.2-proteinNannochloropsis oceanica (N. oceanica IMET1)polypeptide
NO24G00040.1NO24G00040.1-proteinNannochloropsis oceanica (N. oceanica IMET1)polypeptide


The following gene feature(s) are orthologous to this gene:

Feature NameUnique NameSpeciesType
jgi.p|Nanoce1779_2|644232gene_12217Nannochloropsis oceanica (N. oceanica CCMP1779)gene
Naga_101645g1gene9964Nannochloropsis gaditana (N. gaditana B-31)gene


The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
NO24G00040.3NO24G00040.3Nannochloropsis oceanica (N. oceanica IMET1)mRNA
NO24G00040.2NO24G00040.2Nannochloropsis oceanica (N. oceanica IMET1)mRNA
NO24G00040.1NO24G00040.1Nannochloropsis oceanica (N. oceanica IMET1)mRNA


Sequences
The following sequences are available for this feature:

gene sequence

>NO24G00040 ID=NO24G00040|Name=NO24G00040|organism=Nannochloropsis oceanica|type=gene|length=2574bp
GCTAGCTTGAAGTGCTTGTGCTGTGTGCGTGTAGGTTTAGTGTGGTTCGC
GTTATGGGAAGAGAGAAATGAGGCGACCATTGGACCTAAGGCGGCTGAAC
AAAAATTGGGTCGGCAACGCGACTGCTAAGGTGGGAATAAGAATGCGATG
GTGTCGTCGAGCAAGAGAAGAGGAGGGCATCTTTATGTGTGGTGCTATGA
TGTGTGTTGGAGCCGTGGGTTTGGCTTGATGTAATATGGTATGGGAAGGA
AAGGAAGGGAAAGAGGGATGATGACGTTTCTTGAATCCAATGAAATATGA
GCGCATAATTGCAATGTCTCAAAAAATACCATCCACACACTCCCTCGCAT
ACACACCCGCACGCGCGCGCATAGATGCCTCTACCACCTCGCTAGGCTGC
GATGCGACCACGTCCATTTTAAGTCAAAGGAAGACACTCGCTGATCGGTC
GCAGTCTCGTTTGGGCGATCGCCTCTCGCCACCCCTCCCTCCCTCCCAAT
TTGCCGCCTGTGCCTTGCCTCCAGGTTGCGAGGCGTCGCCTAATCGGTTC
CAACCCTCCTTCACAGGGCCCAAATTCCATCCATCAACATGCTTGGCCTC
CTTGTCATCCTCCTACCCGACGAATTCGGCCTTCGGGTAACCCTTAACCC
CCCCTTACCCTGGCCCCATACACACAGGCACACACATGCTGCCCACCCAC
CGCTTCGCGCGTGTATCCTCAATCATCACAAATATTGGAAGCAGAAGAAG
ATACACCTTCTCCTTCTTTCGCTCCTCCTCTGTTTCCCCACCTTCCTCGA
CTTCGTTTTCTTCATTCTCCTCTCACCATCAACATCACGGCCACGATCAC
CATCAACACCACGGCCACGATCACCATCAACACCACGGCCACGACCAAGA
CGAAGACGATGTCCGCCTAATCGCATGCGTGGCCCCTCCTAATCTTACTA
TTGGCAAGGGCGGCCAGCTACCGTGGGATCTACCAGAGgtaggcaaggag
ataagggaaggagggaaagggctgtgacttttgaatgttttgtaagttac
aaaatgtaaagagggaagaagagaatgagagcaagtgcatataaacagtg
acatgtctttgttggtgtctattacttgtacagGACCGGAAGTACTTCTT
TCATTGCACCCAGGGGCACGTGCTGGTGCTGGGCCGACGGTCTTACGAGg
tgcgtgggaaggagggagagacgggagaaagagcaaaagggacttgagcg
agaaagcaggaagagaggtagagagaatttggggtgaactttctgcattg
atcctttattaatgttttcctgatgcatgcagGAAGCCGGGCACGCTCTG
CCAGGCCGAGAGACAGTGGTTGTCTCCCGCACGCTCAGgtaccctccccc
tccctccttcacccttcctctcattccctcgttatcccttcagggggaac
gataatttatatcatatcatattccataaatccatctctgcctctttttg
gtcgccgttcattgagcttttgtgcatgttcatcagcgattcttccctcc
ctccctccctccctgcctccctccctgcctccctgcctgcctgcctccct
ccctcccctcctccctagCTCCCTCCCCGACGCCCTAGTCGCCTCTTCAC
TCACCGAGGCCCTGGCGCTAGCCGGGAAAATCAAAAGGAGAAAGGGGAGA
GAGGGAATGATCACTTGGATCGGAGgtgagggaatggagagggaggaggg
agggagggagggacaggaattgaaggcaagggaggcaggtcgggggttat
atgaaggacgaatttgatatgaaaatctttgaatcgggtcatgataaaag
agaaggaaaaaaggagggaggccagggggaggagagggcgcaatgaaaat
cactttttctctaacctaataatgcgcggacacagGAGGGCATGACCTGT
ACCAGCAGACCATGGCCCTCGACAGGCCCTCGTTCTTGTACTTAACGCAG
ACGCACTTCGAGGTAAGGAAGGGGGAGAGAGTAAAGGTAGGAGGATTGGG
AATTAGTGGGGAGACATTCATACCATGGTTGACAGAAGGAGGTCAGAAAG
AAGTAGGGAATGGCCGCGACGAGAGGAGAAGAATGGAGAGAGAAATGAAG
AAGGAAGGGAGCATGAGAGGAAGAGAGGGACACGGAGAAATGAAGGAAGG
GAGTAAGGAAGAGGATGAAGGAAAGCGGAAGAAGGAGAGGACTGAAAACA
ATAACCAAGTCAACTCCCAACCAAGATACTCCACTACAATGATGCATCTT
GAAACCTACGTCCATATAGGTGCAGGGCGGAGACGCCTTCTTCCCTGAGG
CCTGGGTTGAGTCATTCCCCCGACTCCTGTGGCGTTACAAGTCGTTGAGC
TCGCCTTGCACGTTTGGGATCTACGCGGCGGCAGCGAGATCCCTTCCGGA
GGGATTGAGAGAGGCAGAGTGAGAGGAAATGAGAAGAAAGGGGTCTGGAT
GAGCTCCGATGACATTTCATTGTACACAAAATAGACCATTTGAAAATGCA
TGTACTGACTATAGTGTTAGGTAA
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protein sequence of NO24G00040.3

>NO24G00040.3-protein ID=NO24G00040.3-protein|Name=NO24G00040.3|organism=Nannochloropsis oceanica|type=polypeptide|length=208bp
MLPTHRFARVSSIITNIGSRRRYTFSFFRSSSVSPPSSTSFSSFSSHHQH
HGHDHHQHHGHDHHQHHGHDQDEDDVRLIACVAPPNLTIGKGGQLPWDLP
EDRKYFFHCTQGHVLVLGRRSYEEAGHALPGRETVVVSRTLSSLPDALVA
SSLTEALALAGKIKRRKGREGMITWIGGEGMEREEGGREGQELKAREAGR
GLYEGRI*
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protein sequence of NO24G00040.2

>NO24G00040.2-protein ID=NO24G00040.2-protein|Name=NO24G00040.2|organism=Nannochloropsis oceanica|type=polypeptide|length=315bp
MLPTHRFARVSSIITNIGSRRRYTFSFFRSSSVSPPSSTSFSSFSSHHQH
HGHDHHQHHGHDHHQHHGHDQDEDDVRLIACVAPPNLTIGKGGQLPWDLP
EDRKYFFHCTQGHVLVLGRRSYEEAGHALPGRETVVVSRTLSSLPDALVA
SSLTEALALAGKIKRRKGREGMITWIGGGHDLYQQTMALDRPSFLYLTQT
HFEVRKGERVKVGGLGISGETFIPWLTEGGQKEVGNGRDERRRMEREMKK
EGSMRGREGHGEMKEGSKEEDEGKRKKERTENNNQVNSQPRYSTTMMHLE
TYVHIGAGRRRLLP*
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protein sequence of NO24G00040.1

>NO24G00040.1-protein ID=NO24G00040.1-protein|Name=NO24G00040.1|organism=Nannochloropsis oceanica|type=polypeptide|length=254bp
MLPTHRFARVSSIITNIGSRRRYTFSFFRSSSVSPPSSTSFSSFSSHHQH
HGHDHHQHHGHDHHQHHGHDQDEDDVRLIACVAPPNLTIGKGGQLPWDLP
EDRKYFFHCTQGHVLVLGRRSYEEAGHALPGRETVVVSRTLSSLPDALVA
SSLTEALALAGKIKRRKGREGMITWIGGGHDLYQQTMALDRPSFLYLTQT
HFEVQGGDAFFPEAWVESFPRLLWRYKSLSSPCTFGIYAAAARSLPEGLR
EAE*
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Synonyms
Publications