NO26G00380, NO26G00380 (gene) Nannochloropsis oceanica
Overview
Properties
Mutants
Expression
Hover the mouse over a column in the graph to view expression values. Alignments
The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Annotated Terms
The following terms have been associated with this gene:
Homology
BLAST of NO26G00380 vs. NCBI_GenBank
Match: OLE03924.1 (hypothetical protein AUG82_06415 [Ktedonobacter sp. 13_1_20CM_4_53_11]) HSP 1 Score: 132.1 bits (331), Expect = 4.100e-27 Identity = 124/381 (32.55%), Postives = 166/381 (43.57%), Query Frame = 0 Query: 15 LGLWVNGLRCLAKGGL--SLPEVLAQGEHSFAW-----MLDSGYLTTDGRLLAKPGTPLQEGDLLFLRRSLLLSLLRNRVSSPILHRDQILSLEDEDEGGR-EGGRVRVTLQSGKEEVVDIVVGADGMFSPLVRETTMGLPPPAQRGYTVFRG--VYEGSPLQPGFSFQTWGPSSRFSSVPLADGQV-WFATLAXXXXXXXXXXXXXXXSMEGGREAL---KEGLLQRFGSWHAPVRGLIEGTREALIVKEEAWAQTDFPRGGGREGGRAGRGRRVTLVGDAAHGVDPILAQGAGVAIEDAYHLVYHLAHAQGEGGKEGGDRIAGALSAYEKSTYQRASLLTSISNLSQFIGQLPPGPILSLRDALLPSLPPAFKGRIFDA 382 L LW NG++ L + GL +L V A H W L S L T R + P + D LL LL VS +H + G R E G V G++ D++VGADG++S ++RE +G PP GYT +RG V+E + PG S +TWG RF +P+ +G+V W+AT GG++ K L Q FG W P+ LIE T E I++ + R R RVTL+GDAAH P L QGA A+EDA L LA + AL AYE +R++ + S L IGQ + +LRD L P F R+F A Sbjct: 33 LTLWANGVQMLRQLGLADTLAAVSAALTHFECWSWRGKRLGSMRLDTIERRVGAPSIGIHRAD--------LLRLLAGEVSRESVH------VNAHCVGFRQEQGHVISHFADGQQHQADLLVGADGLYS-VIREQLLGQQPPRYSGYTCWRGVAVFEERHVSPGISSETWGRGRRFGMLPIGNGRVFWYAT---------------YNCPAGGQDQAGERKSRLSQLFGGWREPIEQLIEATDEGAILRNDIL---------DRRPVRHWGSGRVTLLGDAAHPPTPNLGQGACQALEDALVLAGCLADQR---------EPVAALHAYEARRMKRSAAIIEQSYLFGKIGQWEHPLLCALRDGLTPLAFATFLPRLFVA 365
BLAST of NO26G00380 vs. NCBI_GenBank
Match: OLE33151.1 (hypothetical protein AUG45_08150 [Ktedonobacter sp. 13_1_20CM_3_54_15]) HSP 1 Score: 130.6 bits (327), Expect = 1.200e-26 Identity = 124/381 (32.55%), Postives = 165/381 (43.31%), Query Frame = 0 Query: 15 LGLWVNGLRCLAKGGL--SLPEVLAQGEHSFAW-----MLDSGYLTTDGRLLAKPGTPLQEGDLLFLRRSLLLSLLRNRVSSPILHRDQILSLEDEDEGGR-EGGRVRVTLQSGKEEVVDIVVGADGMFSPLVRETTMGLPPPAQRGYTVFRG--VYEGSPLQPGFSFQTWGPSSRFSSVPLADGQV-WFATLAXXXXXXXXXXXXXXXSMEGGREAL---KEGLLQRFGSWHAPVRGLIEGTREALIVKEEAWAQTDFPRGGGREGGRAGRGRRVTLVGDAAHGVDPILAQGAGVAIEDAYHLVYHLAHAQGEGGKEGGDRIAGALSAYEKSTYQRASLLTSISNLSQFIGQLPPGPILSLRDALLPSLPPAFKGRIFDA 382 L LW NG++ L + GL +L V A H W L S L T R + P + D LL LL VS +H + G R E G V G++ D++VGADG+ S ++RE +G PP GYT +RG V+E + PG S +TWG RF +P+ +G+V W+AT GG++ K L Q FG W P+ LIE T E I++ + R R RVTL+GDAAH P L QGA A+EDA L LA + AL AYE +R++ + S L IGQ + +LRD L P F R+F A Sbjct: 47 LTLWANGVQMLRQLGLADTLAAVSAALTHFECWSWRGKRLGSMRLDTIERRVGAPSIGIHRAD--------LLRLLAGEVSRESVH------VNAHCVGFRQEQGHVISHFADGQQHQADLLVGADGLHS-VIREQLLGQQPPRYSGYTCWRGVAVFEERHVSPGISSETWGRGRRFGMLPIGNGRVFWYAT---------------YNCPAGGQDQAGERKSRLSQLFGGWREPIEQLIEATDEGAILRNDIL---------DRRPVRHWGSGRVTLLGDAAHPPTPNLGQGACQALEDALVLAGCLADQR---------EPVAALHAYEARRMKRSAAIIEQSYLFGKIGQWEHPLLCALRDGLTPLAFATFLPRLFVA 379
BLAST of NO26G00380 vs. NCBI_GenBank
Match: PLS84640.1 (hypothetical protein CYG60_16870 [Actinobacteria bacterium]) HSP 1 Score: 121.7 bits (304), Expect = 5.500e-24 Identity = 110/331 (33.23%), Postives = 151/331 (45.62%), Query Frame = 0 Query: 63 GTPLQEGDLLFLRRSLLLSL----LRNRVS--SPILHRDQILSLEDEDEGG-------------REGGRVRVTLQSGKEEVVDIVVGADGMFSPLVRETTMGLPPPAQRGYTVFRGVY--EGSPLQPGFSFQTWGPSSRFSSVPLADGQV-WFATLAXXXXXXXXXXXXXXXSMEGGREALKEGLLQRFGSWHAPVRGLIEGTREALIVKEEAWAQTDFPRGGGREGGRAGRGRRVTLVGDAAHGVDPILAQGAGVAIEDAYHLVYHLAHAQGEGGKEGGDRIAGALSAYEKSTYQRASLLTSISNLSQFIGQLPPGPILSLRDALLPSLP 372 GTP G + R +L S+ L +RV S +HR + +L + G ++ V V L G+ E D++VGADG+ S VR + G P GYT +R V EG L G F+TWG +RF + +G+V WFAT K LL+ F WH PV L+E T EA I +++ + + R G EG RVTL+GDAAH P + QGA +A+EDA LA GE ++G +A AL YE +RA+ + IS IGQL + LRD +L + P Sbjct: 228 GTPASAGVIRSWRGEVLASVPVAKLEDRVGSVSAAVHRADLQALLARELGAGPLRLGSGCAGFEQDERGVTVLLADGRRERADVLVGADGLHS-TVRASLFGQKEPRYAGYTSWRAVARPEGELLPWGQGFETWGRGARFGCAHIGEGRVYWFAT--------RNAPKGEKDGPPXXXXXPKRALLELFRGWHRPVEALVEATEEAEIRRDDLYDREPLKRWG--EG-------RVTLLGDAAHPTTPNIGQGACLAVEDA----VVLARCLGE-QRDGTGGVAEALRRYEGLRRERAAKIVRISRRLGRIGQLENPALRRLRDLVLKAAP 535
BLAST of NO26G00380 vs. NCBI_GenBank
Match: WP_095508678.1 (hypothetical protein [Rubrivirga marina] >PAP75051.1 hypothetical protein BSZ37_00590 [Rubrivirga marina]) HSP 1 Score: 120.2 bits (300), Expect = 1.600e-23 Identity = 114/361 (31.58%), Postives = 160/361 (44.32%), Query Frame = 0 Query: 17 LWVNGLRCLAKGGLSLPEVLAQGEHSFAWMLDSGYLTTDGRLLAKPGTPLQEGDL----LFLRRSLLLSLLRNRVSSPILHRDQILSLEDEDEGGREGGRVRVTLQSGKEEVVDIVVGADGMFSPLVRETTMGLPPPAQRGYTVFRGVYEGSPLQPGFSFQTWGPSSRFSSVPLADGQV-WFATLAXXXXXXXXXXXXXXXSMEGGREALKEGLLQRFGSWHAPVRGLIEGTREALIVKEEAWAQTDFPRGGGREGGRAGRGRRVTLVGDAAHGVDPILAQGAGVAIEDAYHLVYHLAHAQGEGGKEGGDRIAGALSAYEKSTYQRASLLTSISNLSQFIGQLPPGPILSLRDALLPSLPP 373 LW N L L + GL V A + S T DGR L + L + R+ L ++L + P+ +++ +ED EG RVRV + +E D+++GADG+ S VR G PA GYT +RGV + + G RF VPL DG+V WFAT GGR+A ++ L++RF WH VR L+E T E +++ + +D P G GRG R+TL+GDAAH P L QGAG+A+EDA L L + D +A AL YE + R + + S + Q ++ R LL PP Sbjct: 55 LWPNALHALDRLGLG-AAVRAVSQPS----AGGAIWTPDGRPLVRVDPEALAARLGEAPRAIHRADLQAVLADAFDGPLHLGARVIGIED------EGKRVRVRVADDREFEADVLIGADGLHS-AVRSALHGAAAPAYAGYTAWRGVAQADGAV--LDGEAVGRGQRFGQVPLPDGRVYWFATATVP---------------AGGRDAARDELVRRFTEWHPSVRSLVEATPEGAVLRNDV---SDRP-----PLGVWGRG-RITLLGDAAHPSTPNLGQGAGLALEDAAVLADRLGSTR--------DGLA-ALRDYEAARSGRTAAVVRRSRRVGALMQASNPVAVAARTLLLRLTPP 368
BLAST of NO26G00380 vs. NCBI_GenBank
Match: WP_103759951.1 (monooxygenase [Streptomyces sp. SM10]) HSP 1 Score: 119.8 bits (299), Expect = 2.100e-23 Identity = 108/346 (31.21%), Postives = 150/346 (43.35%), Query Frame = 0 Query: 44 AWMLDSGYLTTDGRLLAKPGTPLQE----GDLLFLRRSLLLSLLRNRVSSPILHRDQILSLEDEDEGGREGGRVRVTLQSGKEEVVDIVVGADGMFSPLVRETTMGLPPPAQRGYTVFRGVYEGSPLQPGFSFQTWGPSSRFSSVPLADGQVWFATLAXXXXXXXXXXXXXXXSMEGGREA--LKEGLLQRFGSWHAPVRGLIEGTREALIVKEEAWAQTDFPRGGGREGGRAGRGRRVTLVGDAAHGVDPILAQGAGVAIEDAYHLVYHLAHAQGEGGKEGGDRIAGALSAYEKSTYQRASLLTSISNLSQFIGQLPPGPILSLRDALLPS---LPPAFKGRIFD 381 AW D G T GR LA+ + G L+ L RS L+ LLR R+ + L D GR GGR V + D+VVGADG+ S + G P P+ G+T +R V +P +P S +TWG + + S PL DG+++ A + G R A K LL+RFG WH PV +I+ +++ + T A G RV LVGDAAH + P L QG AIEDA L +H + D + A +AY R + + + + QL P ++LR AL+ + L P R FD Sbjct: 69 AWQGDGGMRTPSGRWLARTDSAAAAERFGGPLVLLPRSALVDLLRARLPDGAVRTAAPAQLVDP---GRTGGRPAVVRTPAGDIEADLVVGADGIRSGVRTALFPGHPGPSYSGFTTWR-VLAPAPGRPFASHETWGRGALWGSHPLKDGRIYAYAAA--------------TAPAGARAADGEKAELLRRFGDWHHPVPDIIDAVEPGQVLRHDVHQMT--------TALPAFHGGRVVLVGDAAHAMAPTLGQGGNQAIEDAVVLAHH--------ARPDVD-LGAAQAAYSADRQPRTTAIVRKAARISRMMQLSAAPAVALRAALMSAASRLGPGLVLRTFD 379
BLAST of NO26G00380 vs. NCBI_GenBank
Match: WP_068420714.1 (hypothetical protein [Planctomyces sp. SH-PL62] >AMV40637.1 FAD-dependent urate hydroxylase [Planctomyces sp. SH-PL62]) HSP 1 Score: 117.9 bits (294), Expect = 7.900e-23 Identity = 118/365 (32.33%), Postives = 159/365 (43.56%), Query Frame = 0 Query: 15 LGLWVNGLRCLAK-GGLSLPEVLAQGEHSFAWMLDSGYLTTDGRLLAKPGTPLQEGDLLFLRRSLLLSLLRN---RVSSPILHRDQILSLEDEDEGGREGGRVRVTLQSGKEEVVDIVVGADGMFSPLVRETTMGLPPPAQRGYTVFRGVYEGSP--LQPGFSFQTWGPSSRFSSVPLADGQV-WFATLAXXXXXXXXXXXXXXXSMEGG---REALKEGLLQRFGSWHAPVRGLIEGTREALIVKEEAWAQTDFPRGGGREGGRAGRGRRVTLVGDAAHGVDPILAQGAGVAIEDAYHLVYHLAHAQGEGGKEGGDRIAGALSAYEKSTYQRASLLTSISNLSQFIGQLPPGPILSLRDALLPS 370 + LW N L L + G L E L + A+ G + D + A P +EG +L L R LL +L + R + IL L ++D G V + G+E D+++GADG+ S VR G PP GYT +R V + P L PG +TWG RF + G+ W+AT EGG + K+GLL F WH P+ LI T E+ I++ + + + R EG RV L+GDAAH + P + QGA AIEDA L LA + G I AL AYE RAS + S + I Q LR+ALL S Sbjct: 42 ISLWRNALAALDRLGVLPALEGLGVSGQTGAFRTPDGSVLLD--MKAGPVDTAREGVILLLHRVELLCILLDVLGRDAPRILRTGARLVAFEQDATG-----VTARFEDGREARGDLLIGADGLRS-AVRAAMFGPEPPRYGGYTAWRAVVDFDPTRLAPG---ETWGRGRRFGQWGMTAGRAYWYATQTVP---------------EGGGDPPQGRKQGLLDLFRDWHEPIADLIRATDESAILRNDVYDRPPLRR--WVEG-------RVALLGDAAHPMTPDMGQGACQAIEDAVALADALA-------RVGPAEIPDALRAYESRRIPRASRIVRASRQAGTIAQWSNPLACKLREALLRS 364
BLAST of NO26G00380 vs. NCBI_GenBank
Match: WP_014397527.1 (FAD-dependent oxidoreductase [Corallococcus coralloides] >AFE05990.1 FAD-dependent oxidoreductase [Corallococcus coralloides DSM 2259]) HSP 1 Score: 117.1 bits (292), Expect = 1.400e-22 Identity = 114/346 (32.95%), Postives = 159/346 (45.95%), Query Frame = 0 Query: 35 VLAQGEHS-FAWMLDSGYLTTDGRLLAKPGTPLQEG---DLLFLRRSLLLSLLRNRVSSPILHRDQILSLEDEDEGGREGGRVRVTLQSGKEEVVDIVVGADGMFSPLVRETTMGLPPPAQRGYTVFRGVYEGSPLQPGFSF-QTWGPSSRFSSVPLADGQV-WFATLAXXXXXXXXXXXXXXXSMEGGREALKEGLL---QRFGSWHAPVRGLIEGTREALIVKEEAWAQTDFPRGGGREGGRAGRGRRVTLVGDAAHGVDPILAQGAGVAIEDAYHLVYHLAHAQGEGGKEGGDRIAGALSAYEKSTYQRASLLTSISNLSQFIGQLPPGPILSLRDALLPSLP 372 V+A+GE + +LDS T R+ A LQE ++ + R+ L ++LR R + ED +G RV VTL +G+ D++VGADG+ S +VR +G P GYT +RGV G+ L P F +TWGP +RF VP+ G+V WFATL + G +A + L RF WHAP+ L+ T +++ + + RG RVTL+GDAAH + P L QG AIED L LA A G + AL AYE +RA+ L S+ + Q G +RDAL +P Sbjct: 67 VVAEGERAEQTLILDS----TGARITAVDVRSLQEELDIPMVAVHRARLQAVLRAHAGPEEAVRLGVSVTGFED----DGARVTVTLSTGETVTGDVLVGADGLRS-VVRTGLLGAQPTRYSGYTSWRGVCPGADLVPAGQFTETWGPGARFGIVPIGHGEVYWFATL---------------NAPAGAEDAPGQTLAVLQDRFAGWHAPIAKLLAATPPERVLRTDIHDRPPV--------SHWSRG-RVTLLGDAAHPMTPNLGQGGCQAIEDGVVLGECLA-APGS--------VEDALRAYESRRVKRANALVVRSHQVGRVAQWENGAARFVRDALFRRVP 370
BLAST of NO26G00380 vs. NCBI_GenBank
Match: WP_094044390.1 (MULTISPECIES: hypothetical protein [Cohnella] >OXS59284.1 hypothetical protein B1A99_11715 [Cohnella sp. CIP 111063] >PRX72306.1 2-polyprenyl-6-methoxyphenol hydroxylase-like FAD-dependent oxidoreductase [Cohnella sp. SGD-V74]) HSP 1 Score: 117.1 bits (292), Expect = 1.400e-22 Identity = 105/353 (29.75%), Postives = 161/353 (45.61%), Query Frame = 0 Query: 52 LTTDGRLLAKPGTP-----LQEGDLLFLRRSLLLSLLRNRVSSPILHRDQILSLEDEDEGGREGGRVRVTLQSGKEEVVDIVVGADGMFSPLVRETTMGLPPPAQRGYTVFRGVYE-GSPLQPGFSF--QTWGPSSRFSSVPLADGQV-WFATLAXXXXXXXXXXXXXXXSMEGGREALKEGLLQRFGSWHAPVRGLIEGTREALIVKEEAWAQTDFPRGGGREGGRAGRGRRVTLVGDAAHGVDPILAQGAGVAIEDAYHLVYHLAHAQGEGGKEGGDRIAGALSAYEKSTYQRASLLTSIS----NLSQFIGQLPPGPILSLRDALLPSLPPAFKGRIFDACIRLSLARGG 392 L+ +G++L++ G P + LR LL L+ + +H + ++ E G V V + G E D++VGADG+ S +VR P GYT +R V + GS L+ +TWG + RF VPL+D ++ WFA + + + L +RF +H+P+ L+E + + I+ + R GRG RV L+GDAAH + P +AQGAG AIEDA L H++ A AL YEK R +T +S ++Q G+LP ++LR+A+LP +P A R D +++L R G Sbjct: 74 LSQEGKVLSRMGAPDRSKNADPEQVTVLRADLLGFLVDALGAGEAIHTGKTCVDFEQGEDG-----VTVKFEDGSTEEADLLVGADGIRS-VVRGKLFPEAKPRYSGYTCWRTVVQAGSSLRYDRELFTETWGSNGRFGIVPLSDNRIYWFACV------------NAPYNDPAMKRVTIADLAKRFAGYHSPISDLLELSSQDAILHHDIEYLPPIK--------RFGRG-RVVLIGDAAHAMTPNMAQGAGQAIEDALFLAEHVSR---------WPNFASALEGYEKDRVSRTGTITRMSGRIGRMAQLEGRLP----VALRNAVLPLVPDALMQRQLDYVYKVNLERAG 386
BLAST of NO26G00380 vs. NCBI_GenBank
Match: AEI66848.1 (FAD-dependent oxidoreductase [Myxococcus fulvus HW-1]) HSP 1 Score: 115.9 bits (289), Expect = 3.000e-22 Identity = 118/343 (34.40%), Postives = 159/343 (46.36%), Query Frame = 0 Query: 48 DSGYLTTDGRLLAK-PGTPLQEG---DLLFLRRSLLLSLLRNRVSSPILHRDQILSLEDEDEGGREGGRVRVTLQSGKEEVVDIVVGADGMFSPLVRETTMGLPPPAQRGYTVFRGVYEGSP-LQPGFSFQTWGPSSRFSSVPLADGQV-WFATLAXXXXXXXXXXXXXXXSMEGGREA---LKEGLLQRFGSWHAPVRGLIEGTREALIVKEEAWAQTDFPRGGGREGGRAGRGRRVTLVGDAAHGVDPILAQGAGVAIEDAYHLVYHLAHAQGEGGKEGGDRIAGALSAYEKSTYQRA-SLLT---SISNLSQFIGQLPPGPILSLRDALLPSLPPAFKGR 378 DS L G L + P +QE L+ + R+ L S+L + + + ++ +D G V V L G D++VGADG+ S +VR +G P GYT +RGV P PG +TWGP +RF VP+ GQ WFAT + GG++A K L FG WHAP+ LI T EA I++ + D P R RG RVTL+GDAAH + P L QG AIEDA L LA GEG + AL+AYE+ RA S +T S+ ++Q+ + P G +RDAL +P R Sbjct: 56 DSAILKPSGAALTRLPVARIQEELGLPLVCIHRARLQSVLLAHAGAENVRLGRTVTAFHDD-----GQAVTVRLSDGSSVTGDVLVGADGLRS-VVRGALLGDAPLRYSGYTSWRGVCADVPSATPGLVSETWGPGARFGVVPIGFGQTYWFAT---------------RNARAGGQDAPGESKARLQSLFGGWHAPIADLIAATDEANILRTDI---HDRP-----PASRWSRG-RVTLLGDAAHPMTPNLGQGGCQAIEDAVALAELLA---GEG------PVDAALAAYEQRRLTRANSFVTRSWSLGRVAQW--ENPAGRF--IRDALFQLVPQGLAAR 355
BLAST of NO26G00380 vs. NCBI_GenBank
Match: WP_043711131.1 (FAD-dependent oxidoreductase [Myxococcus fulvus]) HSP 1 Score: 115.9 bits (289), Expect = 3.000e-22 Identity = 118/343 (34.40%), Postives = 159/343 (46.36%), Query Frame = 0 Query: 48 DSGYLTTDGRLLAK-PGTPLQEG---DLLFLRRSLLLSLLRNRVSSPILHRDQILSLEDEDEGGREGGRVRVTLQSGKEEVVDIVVGADGMFSPLVRETTMGLPPPAQRGYTVFRGVYEGSP-LQPGFSFQTWGPSSRFSSVPLADGQV-WFATLAXXXXXXXXXXXXXXXSMEGGREA---LKEGLLQRFGSWHAPVRGLIEGTREALIVKEEAWAQTDFPRGGGREGGRAGRGRRVTLVGDAAHGVDPILAQGAGVAIEDAYHLVYHLAHAQGEGGKEGGDRIAGALSAYEKSTYQRA-SLLT---SISNLSQFIGQLPPGPILSLRDALLPSLPPAFKGR 378 DS L G L + P +QE L+ + R+ L S+L + + + ++ +D G V V L G D++VGADG+ S +VR +G P GYT +RGV P PG +TWGP +RF VP+ GQ WFAT + GG++A K L FG WHAP+ LI T EA I++ + D P R RG RVTL+GDAAH + P L QG AIEDA L LA GEG + AL+AYE+ RA S +T S+ ++Q+ + P G +RDAL +P R Sbjct: 76 DSAILKPSGAALTRLPVARIQEELGLPLVCIHRARLQSVLLAHAGAENVRLGRTVTAFHDD-----GQAVTVRLSDGSSVTGDVLVGADGLRS-VVRGALLGDAPLRYSGYTSWRGVCADVPSATPGLVSETWGPGARFGVVPIGFGQTYWFAT---------------RNARAGGQDAPGESKARLQSLFGGWHAPIADLIAATDEANILRTDI---HDRP-----PASRWSRG-RVTLLGDAAHPMTPNLGQGGCQAIEDAVALAELLA---GEG------PVDAALAAYEQRRLTRANSFVTRSWSLGRVAQW--ENPAGRF--IRDALFQLVPQGLAAR 375 The following BLAST results are available for this feature:
BLAST of NO26G00380 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes) Total hits: 20
Pagesback to topRelationships
This gene is member of the following syntenic_region feature(s):
The following polypeptide feature(s) derives from this gene:
The following gene feature(s) are orthologous to this gene:
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene sequence >NO26G00380 ID=NO26G00380|Name=NO26G00380|organism=Nannochloropsis oceanica|type=gene|length=2547bpback to top protein sequence of NO26G00380.1 >NO26G00380.1-protein ID=NO26G00380.1-protein|Name=NO26G00380.1|organism=Nannochloropsis oceanica|type=polypeptide|length=428bpback to top Synonyms
Publications
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