NO14G02460, NO14G02460 (gene) Nannochloropsis oceanica

Overview
NameNO14G02460
Unique NameNO14G02460
Typegene
OrganismNannochloropsis oceanica (N. oceanica IMET1)
Sequence length4186
Alignment locationchr14:733236..737421 +

Link to JBrowse

Properties
Property NameValue
DescriptionNitrate reductase
Mutants
The following records are associated with this feature:
TitleMethodAccession dateLast modified date
Cas9 RNP mediated HDR for nitrate reductaseCRISPR/Cas2019-05-102019-05-10
Deletion mutants for nitrate reductaseCRISPR/Cas2019-05-102020-07-30
Expression

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
chr14genomechr14:733236..737421 +
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
PRJNA7699582024-08-13
GSE1786722023-12-15
PRJNA9336932023-10-26
PRJNA9434492023-07-19
PXD0160542021-01-14
PXD0166992021-01-08
GSE1499042020-10-10
NoIMET1_WT_HS2020-09-29
PXD0100302020-03-16
GSE1396152020-03-11
PRJNA2413822020-03-11
BLASTP analysis of N. oceanica IMET1 genes2019-07-11
InterPro analysis for N. oceanica IMET1 genes2019-07-11
GO annotation for IMET1v2 genes2019-07-10
PRJNA1821802019-04-25
Gene prediction for N. oceanica IMET12017-10-24
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0030151molybdenum ion binding
GO:0016491oxidoreductase activity
GO:0005488binding
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0042128nitrate assimilation
GO:0055114oxidation-reduction process
GO:0009987cellular process
GO:0008152metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR036374OxRdtase_Mopterin-bd_sf
IPR017938Riboflavin_synthase-like_b-brl
IPR014756Ig_E-set
IPR036400Cyt_B5-like_heme/steroid_sf
IPR017927Fd_Rdtase_FAD-bd
IPR005066MoCF_OxRdtse_dimer
IPR000572OxRdtase_Mopterin-bd_dom
IPR001433OxRdtase_FAD/NAD-bd
IPR008333OxRdtase_FAD-bd_dom
IPR001199Cyt_B5-like_heme/steroid-bd
IPR001834NADH-Cyt_B5_reductase
IPR008335Mopterin_OxRdtase_euk
IPR001709Flavoprot_Pyr_Nucl_cyt_Rdtase
Homology
BLAST of NO14G02460 vs. NCBI_GenBank
Match: AET85052.1 (nitrate reductase [Nannochloropsis sp. W2J3B])

HSP 1 Score: 1695.6 bits (4390), Expect = 0.000e+0
Identity = 824/875 (94.17%), Postives = 825/875 (94.29%), Query Frame = 0
Query:   20 HDCERCRSSFSTLLLYMAFKLSPQVPTAPEPVRATSTDARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITPAAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELVALPNRTLPVTLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPDALEPGQYWVNFDGPDGELPKGIYGTSIPLLKALDPAQDVLVAFKQNHERLLPDHGFPVRLIIP---------------------------------GYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSVDQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIPLKKGTYKLQGYAYCGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDILRLVGCTNVACRAWDNSQNTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQPVVRIKHPAPIAPGGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIAQRKDLSVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMNAGKDATEDFEAIHSKRAWAMLDEYLVGTLGASLTSSSPEASAIAAPKEAAVALQGKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLGLPVGMHIGLRAVINGESTKRQYTPVSDGDAKGHVELLVKVYRANQHPRFPDGGLMSQHLDRMSLGDCIDIDGPLGHITYEGPGCIRQLGEDVHVKHFVAVAGGTGITPVVQVLRAVLENPCDTTRFSLIYAARVPEDLLLREELDAWAEQYEQFTVHYTVDVPPPDWPYSVGFLTAEMLAANFPEATKDMGALICGPPPMVNFAVKPNLEKLGYTEDQFFIF 862
            H+  RCRSSFSTLLLYMAFKLSPQVPTAPEPVRATSTDARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITPAAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELVALPNRTLPVTLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPDALEPGQYWVNFDGPDGELPKGIYGTSIPLLKALDPAQDVLVAFKQNHERLLPDHGFPVRLIIP                                 GYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSVDQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIPLKKGTYKLQGYAYCGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDILRL               NTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQPVVRIKHPAPIAPGGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIAQRKDLSVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMNAGKDATEDFEAIHSKRAWAMLDEYLVGTLGASLTSSSPEASAIAAPKEAAVALQGKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLGLPVGMHIGLRAVINGESTKRQYTPVSDGDAKGHVELLVKVYRANQHPRFPDGGLMSQHLDRMSLGDCIDIDGPLGHITYEGPGCIRQLGEDVHVKHFVAVAGGTGITPVVQVLRAVLENPCDTTRFSLIYAARVPEDLLLREELDAWAEQYEQFTVHYTVDVPPPDWPYSVGFLTAEMLAANFPEATKDMGALICGPPPMVNFAVKPNLEKLGYTEDQFFIF
Sbjct:    6 HNEGRCRSSFSTLLLYMAFKLSPQVPTAPEPVRATSTDARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITPAAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELVALPNRTLPVTLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPDALEPGQYWVNFDGPDGELPKGIYGTSIPLLKALDPAQDVLVAFKQNHERLLPDHGFPVRLIIPGTQSNIXXXXXXXXXXXEPQPYYSLSFLSPLLAGYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSVDQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIPLKKGTYKLQGYAYCGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDILRL---------------NTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQPVVRIKHPAPIAPGGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIAQRKDLSVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMNAGKDATEDFEAIHSKRAWAMLDEYLVGTLGASLTSSSPEASAIAAPKEAAVALQGKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLGLPVGMHIGLRAVINGESTKRQYTPVSDGDAKGHVELLVKVYRANQHPRFPDGGLMSQHLDRMSLGDCIDIDGPLGHITYEGPGCIRQLGEDVHVKHFVAVAGGTGITPVVQVLRAVLENPCDTTRFSLIYAARVPEDLLLREELDAWAEQYEQFTVHYTVDVPPPDWPYSVGFLTAEMLAANFPEATKDMGALICGPPPMVNFAVKPNLEKLGYTEDQFFIF 865          
BLAST of NO14G02460 vs. NCBI_GenBank
Match: EWM20573.1 (nitrate reductase [Nannochloropsis gaditana])

HSP 1 Score: 926.0 bits (2392), Expect = 8.500e-266
Identity = 436/611 (71.36%), Postives = 496/611 (81.18%), Query Frame = 0
Query:   36 MAFKLSPQVPTAPEPVRATSTDARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITPAAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELVALPNRTLPVTLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPDALEPGQYWVNFDGPDGELPKGIYGTSIPLLKALDPAQDVLVAFKQNHERLLPDHGFPVRLIIPGYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSVDQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIP-LKKGTYKLQGYAYCGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDILRLVGCTNVACRAWDNSQNTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQPVVRIKHPAPIAPGGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIAQRKDLSVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMNAGKDATEDFEAIHSKRAWAMLDEYLVGTLGASL--TSSSP----EASAIAAPKEAAVALQGKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLG 640
            MAF  SP +PT P P+  + TDA D+DT DKWV RLP MIRLTGRHPFNAEP TK LV+ GFITPAAMHYVRNHGPVP+L+W+ HRITV+G+GV +P   SMDEL++LP RTLPVTLVCAGNRRKEVNV R+SKGF+WG+GAVS S+WTGVPLHVLL  CG++   L PG YWVNFDGPDGELPKG+YGTSIPL KALDPAQDVLVA KQNHE L+PDHGFPVR+IIPGYIGGRMIKWLTRITIS + SQSFYH+HDNRVLPSSVDQERAD E WWRKPEYIINDLNLNSAITHP+H+EEIP L+ GTYK+QGYAYCGGGR+V R+EVSLDDG+SWEL ++S EEYPTEHGRFW WR+W+LDVD+LRL    +VACRAWD+SQNTQPRDLTWNVLGMMNNSWFRL T++  N +QQ V+ + HPAPI   GWME G   T     K  G   G  HVE+KS+P    RKDL  I+  ELARH S+TDCWIA+KG VY+VTPYL+EHPGGV AIVMN GKDATEDFEAIHS+ AW MLD+YLVG L  S   TSS P       A A+   A VALQGK + +KCKL+ KEY +PDVLR++F LP  +  LG
Sbjct:    1 MAFNTSPVLPTVPPPIHPSFTDAHDEDTVDKWVHRLPDMIRLTGRHPFNAEPDTKALVNQGFITPAAMHYVRNHGPVPQLSWESHRITVSGVGVPQPHTFSMDELLSLPQRTLPVTLVCAGNRRKEVNVHRKSKGFNWGAGAVSNSLWTGVPLHVLLEQCGLNARDLPPGSYWVNFDGPDGELPKGVYGTSIPLRKALDPAQDVLVAHKQNHEPLMPDHGFPVRMIIPGYIGGRMIKWLTRITISHEPSQSFYHYHDNRVLPSSVDQERADREDWWRKPEYIINDLNLNSAITHPSHNEEIPLLENGTYKIQGYAYCGGGRRVHRVEVSLDDGQSWELVEMSKEEYPTEHGRFWSWRLWHLDVDVLRLASGKSVACRAWDDSQNTQPRDLTWNVLGMMNNSWFRLKTSLHRNTQQQLVLHVHHPAPIGSLGWMEKGTGVTAMAPGKEQG---GAQHVEEKSIPVREIRKDLPAISSTELARHKSRTDCWIAIKGLVYNVTPYLKEHPGGVGAIVMNGGKDATEDFEAIHSRTAWRMLDKYLVGVLSPSSPGTSSPPSLRTSGGAPASIPAAKVALQGKGKKLKCKLLEKEYAAPDVLRLKFALPHGNATLG 608          
BLAST of NO14G02460 vs. NCBI_GenBank
Match: XP_020202176.1 (inducible nitrate reductase [NADH] 2 [Cajanus cajan])

HSP 1 Score: 799.3 bits (2063), Expect = 1.200e-227
Identity = 404/826 (48.91%), Postives = 536/826 (64.89%), Query Frame = 0
Query:   51 VRATSTDARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITPAAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELVA-LPNRTLPVTLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPDALEPGQYWVNFDGPDGELPKG---IYGTSIPLLKALDPAQDVLVAFKQNHERLLPDHGFPVRLIIPGYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSVDQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIPLKKGT----YKLQGYAYCGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDILRLVGCTNVACRAWDNSQNTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQPVVRIKHP--APIAPGGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIAQRKDLSVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMNAGKDATEDFEAIHSKRAWAMLDEYLVG---TLGASLTSSSP-----EASAIAAPKEA----AVALQGKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLGLPVGMHIGLRAVINGESTKRQYTPVSDGDAKGHVELLVKVYRANQHPRFPDGGLMSQHLDRMSLGDCIDIDGPLGHITYEGPGCIRQLGEDVHVKHFVAVAGGTGITPVVQVLRAVLENPCDTTRFSLIYAARVPEDLLLREELDAWAEQYEQFTVHYTVDVPPPD-WPYSVGFLTAEMLAANFPEATKDMGALICGPPPMVNFAVKPNLEKLGY 854
            V A+  D RDD T+D W++R   + RLTG+HPFN+EP    L+  GFITP  +HYVRNHGPVP+  W+D  + VTGL VA P   +MD+L+   P+R  P TLVCAGNRRKE N+ +QS GF+WG+ AVSTS+W GVPL  LL+ CG+   +   G   V F+G + +LP G    YGTSI    ALDP++D+++A+ QN ERL PDHGFPVR+IIPG+IGGRMIKWL RI ++ Q+SQS+YH+ DNRVLPS VD E A+ E WW KPEYIIN+LN+NS IT P HDE +P+   T    Y L+GYAY GGGR+V R+EV+LD G++W++  L   E P ++G++WCW  W+L+V++L L+G   +A RAWD + NTQP  L WNV+GMMNN WFR+ T V    + +  +  +HP  A    GGWM          + K     S  + +  KSV S          +  E+ +HNS    WI V G VYD T +L++HPGG  +I++NAG D TE+FEAIHS +A  ML++Y +G   T G +  SSSP     +A+ +A  KE     +VAL  + +   CKL+ K   S DV   RF LP  DQ LGLPVG H+ L A ++ +   R YTP S  D  G+ +L+VKVY    HP+FP+GGLMSQHLD + +G  +D+ GPLGHI Y G G     G+         +AGGTGITP+ QV +A+L++P D T+  ++YA R  +D+LLREELD WA+++++F V Y V     + W YSVGF+T  +L  + P A+ D  AL CGPPPM+ FAV+PNLEKLGY
Sbjct:   64 VEASVLDPRDDGTSDHWIERNASLFRLTGKHPFNSEPPLPRLMHHGFITPVPLHYVRNHGPVPRARWEDWTVEVTGL-VARPTRFTMDQLLRDFPSREFPATLVCAGNRRKEQNMVKQSIGFNWGAAAVSTSVWRGVPLRALLKRCGI--YSRTKGALHVCFEGAE-DLPGGGGSKYGTSIMREVALDPSRDIILAYMQNGERLTPDHGFPVRMIIPGFIGGRMIKWLKRIIVTTQQSQSYYHYQDNRVLPSHVDAELANAEAWWYKPEYIINELNINSVITTPCHDEILPINSWTTQIPYLLRGYAYSGGGRKVTRVEVTLDGGETWQVCTLDCPEKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDEALNTQPEKLIWNVMGMMNNCWFRVKTNVCKAHKGEIGIVFEHPTQAGNQSGGWM---------AKEKHLEKSSESNPILKKSVSSPFMNTTSKTYSISEVRKHNSPDSAWIIVHGYVYDCTRFLKDHPGGTDSILINAGTDCTEEFEAIHSDKAKKMLEDYRIGELMTTGYTSDSSSPNTTVHDATHLAPIKEVTPQRSVALTPREKT-PCKLLSKTSLSHDVRLFRFSLPSEDQLLGLPVGKHLFLCATVDEKLCMRAYTPTSSVDEVGYFDLVVKVYFRGVHPKFPNGGLMSQHLDSLPIGSVLDVKGPLGHIEYTGRGNFLVHGKPRFATRLAMLAGGTGITPIYQVAQAILKDPEDRTQMHVVYANRTQDDILLREELDDWAQKHDRFKVWYVVQESLREGWEYSVGFITESILREHVPHASPDTLALTCGPPPMIQFAVQPNLEKLGY 875          
BLAST of NO14G02460 vs. NCBI_GenBank
Match: PRW21069.1 (nitrate reductase [Chlorella sorokiniana])

HSP 1 Score: 795.8 bits (2054), Expect = 1.300e-226
Identity = 395/829 (47.65%), Postives = 519/829 (62.61%), Query Frame = 0
Query:   54 TSTDARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITPAAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELVALPNRTLPVTLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPDALEPGQYWVNFDGPDGELPK---GIYGTSIPLLKALDPAQDVLVAFKQNHERLLPDHGFPVRLIIPGYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSVDQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIPLKKGTYKLQGYAYCGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDILRLVGCTNVACRAWDNSQNTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQPVVRIKHPAPIA--PGGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIAQRKDLSVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMNAGKDATEDFEAIHSKRAWAMLDEYLVGTLGASLTSSSPEASAIAAPKEAAVALQ----------------GKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLGLPVGMHIGLRAVINGESTKRQYTPVSDGDAKGHVELLVKVYRANQHPRFPDGGLMSQHLDRMSLGDCIDIDGPLGHITYEGPGCIRQLGEDVHVKHFVAVAGGTGITPVVQVLRAVLENPCDTTRFSLIYAARVPEDLLLREELDAWAEQYEQFTVHYTVDVPPPDWPYSVGFLTAEMLAANFPEATKDMGALICGPPPMVNFAVKPNLEKLGYTEDQFFIF 862
            T T   D+ T D W+ R P +IRLTGRHPFN EP    L ++ FITP ++HYVRNHG VP++ W++HR+TV GL V +P   +MDEL+ LP+  +  TL CAGNRRKE N+ +Q+ GF+WG    S S WTGV L  +LR CGV   ++E G ++V F GP GELPK   G YGTSI   KA+DPA DV++A+KQN   L PDHG+P+RLIIPGYIGGRMIKWL  I+++  ESQ+ YHFHDNRVLPS VD+  A+ EGWW  P++IINDLN+ SAI +P HDE +PL  GTY ++GYAYCG G ++ R E+SLDDGK+W LA ++    P  +G+ W W  W+++V I  L+ C  + CRAWD+S NTQP   TWN++GM++N  +R+       +     ++ +HP   A   GGWM    D+   V A  +  GSG         P+        + T  E+  HN+K   W  V G+VYD TP+L+EHPGG  +I++ AG DAT++F AIHS +A  ML +Y +G L       +PE    AAP                           + I  KLV KE  S +V R RF L  P    GLPVG H+ L A INGE   R YTP S  D +G+ EL+VK+Y ANQHPRFPDGG MSQ+L+ +++GDCI+  GP+GH+ Y G G     GE    +H   +AGGTGITP+ QV++AVL++  D+T+  L+YA   P+D+LLREELD  A  +  F+V YTVD     WP+S GF+  +M+      A  D  A++CGPPPM+ FA  PNLEKLGYT +Q   F
Sbjct:   51 TDTHPHDEGTKDAWIPRHPDLIRLTGRHPFNCEPTPARLFES-FITPPSLHYVRNHGAVPRIKWEEHRLTVNGL-VNKPATFTMDELLQLPSVDVTCTLTCAGNRRKEQNMVKQTIGFNWGPAGTSCSTWTGVRLADVLRLCGV--KSMEEGAHYVCFRGPKGELPKGDDGSYGTSITWGKAMDPASDVILAYKQNGRLLTPDHGYPLRLIIPGYIGGRMIKWLEEISVTEIESQNHYHFHDNRVLPSHVDEALANKEGWWYNPDFIINDLNVQSAIGYPAHDEVVPLTAGTYAVRGYAYCGNGNKIIRCELSLDDGKTWRLADVTHRTQPNTYGKHWAWVWWSIEVPIAELLTCPEIMCRAWDSSMNTQPNTFTWNLMGMLSNCCYRVKIHPRQTNTGGFALQFEHPTIAAGTVGGWMNRAEDQASTVAAPVS-AGSG---------PATPGSGSTQLFTMAEVEAHNTKESAWFVVDGKVYDATPFLKEHPGGADSILLVAGTDATDEFNAIHSAKAKKMLLDYHIGDL-------APEGVIPAAPAXXXXXXXXXXXXXXXXXXXXXXLDPRKRIPFKLVEKEELSHNVRRFRFALQSPQHRFGLPVGKHVFLYAKINGELVMRAYTPSSSDDERGYFELVVKIYFANQHPRFPDGGKMSQYLESLAIGDCIEAKGPIGHVEYLGRGRYSLDGEPHFARHISMIAGGTGITPMYQVIKAVLKDADDSTQLKLLYANVSPDDILLREELDQLAAAHPNFSVWYTVDNADEGWPFSTGFINEDMVRERLFPAGPDTIAVLCGPPPMLKFACLPNLEKLGYTPEQCIQF 858          
BLAST of NO14G02460 vs. NCBI_GenBank
Match: XP_024018107.1 (nitrate reductase [NADH] [Morus notabilis])

HSP 1 Score: 795.0 bits (2052), Expect = 2.300e-226
Identity = 403/834 (48.32%), Postives = 529/834 (63.43%), Query Frame = 0
Query:   57 DARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITPAAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELV-ALPNRTLPVTLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPDALEPGQYWVNFDGPDGELPKG---IYGTSIPLLKALDPAQDVLVAFKQNHERLLPDHGFPVRLIIPGYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSVDQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIPLKKGT----YKLQGYAYCGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDILRLVGCTNVACRAWDNSQNTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQPVVRIKHPAPIA--PGGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIAQRKDLSVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMNAGKDATEDFEAIHSKRAWAMLDEYLVG---TLGASLTSSSPEASA---------------IAAPKEAAVALQGKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLGLPVGMHIGLRAVINGESTKRQYTPVSDGDAKGHVELLVKVYRANQHPRFPDGGLMSQHLDRMSLGDCIDIDGPLGHITYEGPGCIRQLGEDVHVKHFVAVAGGTGITPVVQVLRAVLENPCDTTRFSLIYAARVPEDLLLREELDAWAEQYEQFTVHYTVDVPPPDWPYSVGFLTAEMLAANFPEATKDMGALICGPPPMVNFAVKPNLEKLGY-TEDQFFIF 862
            D RDD T+D W++R   M+RLTG+HPFN+EP    L+  GFITP  +HYVRNHG VP+ +WDD  + VTGL V  P   +MD+LV   P+R  PVTLVCAGNRRKE N+ +QS GF+WG+ AVSTS+WTGVPL  LL+ CG+ P   E G   V F+G + +LP G    YGTS+    A+DPA+D+++A+ QN ERL PDHGFPVR+IIPG+IGGRM+KWL RI ++ QES S+YH+ DNRVLPS VD E A+ E WW KPEYIIN+LN+NS IT P H E +P+   T    Y L+GYAY GGG++V R+EV++D G++W +  L   E P ++G++WCW  W+L+V++L L+    +A RAWD + NTQP  L WNV+GMMNN WFR+ T V    + +  +  +HP       GGWM A      + +AK+  T         KS  S          +  E+ +HNS    WI V G +YD T ++++HPGG  +I++NAG D TE+F+AIHS +A  +L++Y +G   T G +  SSSP  S                I  P    VAL  + + I CKLV K   S DV   RF LP   Q LGLPVG HI L AVING+   R YTP +  D  G+ EL+VKVY  N HP+FP+GG+MSQ+LD + LG  +D+ GPLG I Y G G     G+    K    +AGGTGITP+ QV++A+L++P D T   ++YA R  +D+LLREELD WA   E+F V Y V+     W YS GF+T  +L  + PE + D  AL CGPPPM+ FAV+PNLEK+ Y  +D   IF
Sbjct:   89 DPRDDGTSDSWIERNSTMVRLTGKHPFNSEPPLTRLMSHGFITPVPLHYVRNHGAVPRASWDDWTVEVTGL-VKRPAKFTMDQLVNDFPSREFPVTLVCAGNRRKEQNLVKQSIGFNWGAAAVSTSVWTGVPLRALLKRCGILP--REKGALNVCFEGAE-DLPGGGGSKYGTSVRKEIAMDPARDIILAYMQNRERLKPDHGFPVRMIIPGFIGGRMVKWLKRIVVTTQESDSYYHYKDNRVLPSHVDAELANAESWWYKPEYIINELNINSVITTPCHQEILPINSWTTQMPYTLKGYAYSGGGKKVARVEVTMDGGETWHVCTLDHPEKPNKYGKYWCWCFWSLEVEVLNLLVAKEIAVRAWDETINTQPEKLIWNVMGMMNNCWFRVKTNVCKPHKGEIGIIFEHPTQPGNQSGGWM-AKERHLESTEAKSNPT-------LKKSGSSPFMNTAAKTYSMSEVKKHNSPNSAWIIVHGHIYDCTRFIKDHPGGADSILINAGTDCTEEFDAIHSDKAKNLLEDYRIGELLTTGYASDSSSPNNSVHGSSNALSHLAPIKEITPPVSRPVALVPREK-IPCKLVKKTSISHDVRVFRFALPSEGQVLGLPVGKHIFLCAVINGKLCMRAYTPTTSIDEIGYFELVVKVYFKNVHPKFPNGGMMSQYLDSLPLGSALDVKGPLGDIEYMGRGQFSVHGKPKFAKRLAMLAGGTGITPIYQVVQAILKDPEDDTEMYVVYANRTEDDILLREELDEWARTCERFKVWYVVEKGGEGWQYSTGFVTESILREHIPEGSDDTLALACGPPPMIQFAVQPNLEKMNYDIKDSLLIF 909          
BLAST of NO14G02460 vs. NCBI_GenBank
Match: XP_019416847.1 (PREDICTED: inducible nitrate reductase [NADH] 2-like [Lupinus angustifolius])

HSP 1 Score: 793.9 bits (2049), Expect = 5.000e-226
Identity = 404/842 (47.98%), Postives = 543/842 (64.49%), Query Frame = 0
Query:   40 LSPQVPTAPEPVRATSTDARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITPAAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELV-ALPNRTLPVTLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPDALEPGQYWVNFDGPD---GELPKGIYGTSIPLLKALDPAQDVLVAFKQNHERLLPDHGFPVRLIIPGYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSVDQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIPLKKGT----YKLQGYAYCGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDILRLVGCTNVACRAWDNSQNTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQPVVRIKHPA-PIAP-GGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIAQRKDLSVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMNAGKDATEDFEAIHSKRAWAMLDEYLVGTL-----GASLTSSSP--------EASAIAAPKEAAVALQ----GKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLGLPVGMHIGLRAVINGESTKRQYTPVSDGDAKGHVELLVKVYRANQHPRFPDGGLMSQHLDRMSLGDCIDIDGPLGHITYEGPGCIRQLGEDVHVKHFVAVAGGTGITPVVQVLRAVLENPCDTTRFSLIYAARVPEDLLLREELDAWAEQYEQFTVHYTVDVPPPD-WPYSVGFLTAEMLAANFPEATKDMGALICGPPPMVNFAVKPNLEKLGY 854
            L+  V      +  +  D RDD T D W+QR   +IRLTG+HP+N+EP    L+  GFITP  +HYVRNHGPVP++ WDD  + VTGL V  P   +MD+L+   P+R LPVTLVCAGNRR E N+ +QS GF+WG+ AVSTS+W GVPL+ +L+ CG+       G  +V F+G D   G+     YGTSI    ALDP++D+++A+ QN ERL+PDHGFPVR+IIPG+IGGRM+KWL RI ++ Q+S S+YH+ DNRVLPS VD E A+ E WW KPEYIIN+LN+NS IT P HDE +P+   T    Y L+GYAY GGGR+V R+EV+LD G++W L+ L++EE P ++G++WCW +W+L+V++L ++G   +A RAWD + NTQP+ L WNV+GMMNN WFR+ + +    + +  +  +HP  P  P GGWM   A E    ++  T T    ++++ KSV S          +  ++ +H++    WI V GQVYD T +L+ HPGG  +I++NAG D TE+F+AIHS +A  ML+ Y +G L      +  ++SSP        E + +A  KE  V  Q         I CKLV K   S DV   RF LP   Q LGLPVG HI L A IN +   R YTP S  D  G+ +LLVK+Y  N HP+FP+GGLMSQHLD + +G  +D+ GPLGHI Y G G     GE    K    +AGGTGITP+ QV +A+L++P D T   +IYA R  +D+LL EELD W++ Y++F V Y V     + W Y+VGF+T  ++  + PEA+ D  AL CGPPPM+ FAV+PNLEKLGY
Sbjct:   55 LNDLVSKGTTEIETSIYDPRDDGTPDHWIQRNTTLIRLTGKHPYNSEPPLPRLMHHGFITPVPVHYVRNHGPVPRIRWDDWTMEVTGL-VNRPTQFTMDQLINNFPSRELPVTLVCAGNRRMEQNLVKQSIGFNWGAAAVSTSVWKGVPLYSVLKQCGIYNRT--KGALYVCFEGADDLPGDGGGTKYGTSIMREAALDPSRDIILAYMQNGERLIPDHGFPVRIIIPGFIGGRMVKWLKRIIVTTQQSDSYYHYRDNRVLPSHVDAELANAEAWWYKPEYIINELNINSVITTPCHDEILPINSWTTQRPYVLKGYAYSGGGRKVTRVEVTLDGGETWHLSTLNNEEKPNKYGKYWCWCLWSLEVEVLEILGAKEIAVRAWDEALNTQPQKLIWNVMGMMNNCWFRVKSNMCKPHKGEIGIVFEHPTQPGNPSGGWM---AKERHLEKSSYTST----TYLK-KSVSSPFMNTTSKTFSISQVNKHSNPDSAWIIVHGQVYDCTTFLKYHPGGKDSILINAGTDCTEEFDAIHSNKAKEMLESYRIGELVTTGYTSDSSTSSPNNTVHGNSEFTHLATIKEVTVPSQSIALNPREKIPCKLVSKTSISHDVRLFRFALPSNAQLLGLPVGKHIFLCATINDKLCMRAYTPTSAVDELGYFDLLVKIYFKNVHPKFPNGGLMSQHLDSLPVGSFLDVKGPLGHIQYTGKGNFLVHGEHKFAKRLAMLAGGTGITPIYQVAQAILKDPTDETEMHVIYANRTEDDILLMEELDDWSKNYDRFKVWYVVQESIREKWKYNVGFITESVIREHLPEASNDTLALTCGPPPMIQFAVQPNLEKLGY 885          
BLAST of NO14G02460 vs. NCBI_GenBank
Match: ADV03138.1 (nitrate reductase 1 [Medicago truncatula])

HSP 1 Score: 793.5 bits (2048), Expect = 6.600e-226
Identity = 389/828 (46.98%), Postives = 539/828 (65.10%), Query Frame = 0
Query:   57 DARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITPAAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELV-ALPNRTLPVTLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPDALEPGQYWVNFDGPDGELPKG---IYGTSIPLLKALDPAQDVLVAFKQNHERLLPDHGFPVRLIIPGYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSVDQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIPLKKGT----YKLQGYAYCGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDILRLVGCTNVACRAWDNSQNTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQPVVRIKHPAPIA--PGGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIAQRKDLSVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMNAGKDATEDFEAIHSKRAWAMLDEYLVGTLGASLTSSSPEAS--------------------AIAAPKEAAVALQGKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLGLPVGMHIGLRAVINGESTKRQYTPVSDGDAKGHVELLVKVYRANQHPRFPDGGLMSQHLDRMSLGDCIDIDGPLGHITYEGPGCIRQLGEDVHVKHFVAVAGGTGITPVVQVLRAVLENPCDTTRFSLIYAARVPEDLLLREELDAWAEQYEQFTVHYTVDVPPPD-WPYSVGFLTAEMLAANFPEATKDMGALICGPPPMVNFAVKPNLEKLGY 854
            D RD  T+D+W++R   M+RLTG+HPFN+EP  +ELV+ GFITP  +HYVRNHGPVPK  WD   I V+GL V  P   +M +L+   P+   PVTLVCAGNRRKE N+ +QS GF+WG+  +STS+W GVPL  +L+ CG+       G  +V F+G + ELP G    YGTSI    ALDP++D+++A+ QN + L PDHGFPVR+IIPG+IGGRM+KWL RI ++ ++S ++YH++DNRVLPS VD E A+ EGWW KPEYIIN+LN+NS IT P HDE +P+   T    Y L+GYAY GGGR+V R+EV++D G++W++  L   E P ++G++WCW  W+L+V++L L+G  ++A RAWD S NTQP++L WN++GMMNN WF++ +++   ++ +  +  +HP       GGWM          + +     S  + +  KS+ S          T  E+ +HN+    WI V G VYD T ++++HPGG  +I++NAG D TE+FEAIHS +A  ++++Y +G L  + + SSP+ S                     +   +  +VAL  + +   CKL+FK+  S DV   RF LP   Q LGLPVG HI L A I+ +   R YTP S  D KGH +L+VK+Y    HP+FP+GGLMSQHLD M +G  +DI GPLGHI Y G G     G+    K    +AGG+GITP+ QV++A+L++P D T   ++YA R  +D+LLREE+D+WA+++EQF V Y V     + W YSVGF+T  +L  + P+A+ D  AL+CGPPPMV FAVKPNLEKLGY
Sbjct:   80 DPRDAGTSDQWIKRNTSMLRLTGKHPFNSEPPLQELVEHGFITPVPLHYVRNHGPVPKAQWDSWTIEVSGL-VKNPTQFTMAQLIDDFPSHEFPVTLVCAGNRRKEQNMIKQSIGFNWGAAGISTSVWRGVPLCTVLKSCGI--LNRNSGALYVCFEGAE-ELPGGGGSKYGTSITREVALDPSRDIILAYMQNGDVLTPDHGFPVRIIIPGFIGGRMVKWLKRIIVTSKQSDNYYHYNDNRVLPSHVDAELANAEGWWYKPEYIINELNVNSVITSPCHDEILPINSCTTQMPYLLRGYAYSGGGRKVTRVEVTMDGGETWQVCTLDHSEKPNKYGKYWCWCFWSLEVEVLDLLGAKDIAVRAWDESLNTQPQNLIWNLMGMMNNCWFKVKSSLCKANKGEIGILFEHPTQPGNQSGGWM---------AKERQLEKSSDSNSILKKSLSSPFMITSSKTYTISEVEKHNNAESAWIIVHGHVYDCTRFIKDHPGGADSILINAGTDCTEEFEAIHSDKAKKIIEQYRIGELITTGSYSSPDISMHNTSVSVTTNLAPIKEITLPVTPLRSVSVALNPREKT-SCKLLFKKSISHDVRLFRFALPYEGQLLGLPVGKHIFLCATIDEKLCMRAYTPTSSVDEKGHFDLVVKIYLKGVHPKFPNGGLMSQHLDSMPIGSTLDIKGPLGHIEYAGRGNFMVHGKHKFAKRLAMLAGGSGITPIYQVVQAILKDPEDLTELHVVYANRSEDDILLREEMDSWAKKHEQFKVWYVVQESKREGWEYSVGFITESILKQHVPQASPDTLALVCGPPPMVQFAVKPNLEKLGY 893          
BLAST of NO14G02460 vs. NCBI_GenBank
Match: XP_013708150.1 (nitrate reductase [NADH], clone PBNBR1412-like [Brassica napus])

HSP 1 Score: 793.1 bits (2047), Expect = 8.600e-226
Identity = 400/831 (48.13%), Postives = 535/831 (64.38%), Query Frame = 0
Query:   57 DARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITPAAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELVA-LPNRTLPVTLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPDALEPGQYWVNFDGPDGELPKG---IYGTSIPLLKALDPAQDVLVAFKQNHERLLPDHGFPVRLIIPGYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSVDQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIPLKKGT----YKLQGYAYCGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDILRLVGCTNVACRAWDNSQNTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQPVVRIKHPAPIA--PGGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIAQRKDLSVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMNAGKDATEDFEAIHSKRAWAMLDEYLVGTLGASLTSSSPEASA---------IAAPKEAA----VALQGKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLGLPVGMHIGLRAVINGESTKRQYTPVSDGDAKGHVELLVKVYRANQHPRFPDGGLMSQHLDRMSLGDCIDIDGPLGHITYEGPGCIRQLGEDVHVKHFVAVAGGTGITPVVQVLRAVLENPCDTTRFSLIYAARVPEDLLLREELDAWA-EQYEQFTVHYTVDVPPPDWPYSVGFLTAEMLAANFPEATK-DMGALICGPPPMVNFAVKPNLEKLGY-TEDQFFIF 862
            D+RD+ TAD W+ R   M+RLTG+HPFNAE     L+  GFITP  +HYVRNHGPVPK  W +  I VTGL V  P +L+M++L++  P+R  PVTLVCAGNRRKE N+ +Q+ GF+WGS  VSTS+W GVPL  +LR CGV   +   G   V F+G + +LP G    YGTSI    A+DPA+D+++A+ QN E L PDHGFPVR+I+PG+IGGRM+KWL RI ++ QES S+YH+ DNRVLPS VD E A+ E WW KPEYIIN+LN+NS IT P H+E +P+   T    Y L+GYAY GGG++V R+EV+LD G++W + +L  +E P ++G+FWCW  W+LDV++L L+   +VA RAWD S NTQP  L WN++GMMNN WFR+ T V    R +  +  +HP       GGWM       ++ ++  T           KSV S        + +  ++ +HNS    WI V G VYD T +L++HPGG+ +I++NAG D TE+FEAIHS +A  +L++Y +G L  +   SSP  S          +A  KE A    +AL      +  KL+ K   S DV R RF LP  DQ LGLPVG HI L A IN +   R YTP S  DA GH++L+VKVY    HPRFP+GGLMSQHLD +S+G  +DI GPLGHI Y+G G     G+    K    +AGGTGITP+ QV++++L +P D T   ++YA R  +D+L+REEL+ WA +  E+  V Y V++    W YS GF+T ++L  + PE  + +  AL CGPPPM+ FA++PNLEK+GY  ++   IF
Sbjct:   90 DSRDESTADNWIHRNSSMVRLTGKHPFNAEAPLPRLMHHGFITPVPLHYVRNHGPVPKADWSEWTIEVTGL-VKRPVILTMEQLISEFPSREFPVTLVCAGNRRKEQNMVKQTIGFNWGSAGVSTSLWKGVPLSEILRRCGV--YSRRGGALNVCFEGAE-DLPGGGGAKYGTSIKKEMAMDPARDIILAYMQNGELLTPDHGFPVRIIVPGFIGGRMVKWLKRIIVTPQESDSYYHYKDNRVLPSLVDAELANAEAWWYKPEYIINELNINSVITTPGHEEILPINAFTTQKPYTLRGYAYSGGGKKVTRVEVTLDGGETWSVCELDHQEKPNKYGKFWCWCFWSLDVEVLDLLSAKDVAVRAWDESFNTQPDKLIWNLMGMMNNCWFRIRTNVCKPHRGEIGIVFEHPTRPGNQSGGWMAKERQLEISSESNPT---------LKKSVSSPFMNTSSKMYSISDVRKHNSVESAWIIVHGHVYDCTRFLKDHPGGIDSILINAGTDCTEEFEAIHSDKAKKILEDYRIGELITTGYDSSPNVSVHGGSNVAPLLAPIKELAPPKNIALVNPREKVPVKLIEKTSISHDVRRFRFALPSEDQQLGLPVGKHIFLCANINDKLCLRAYTPTSTVDAVGHIDLVVKVYFKQVHPRFPNGGLMSQHLDSLSIGAVLDIKGPLGHIEYKGKGNFMVNGKPKFAKKLAMLAGGTGITPIYQVIQSILSDPEDETEMYVVYANRTEDDILVREELEGWANKDKERLKVWYVVEIAKEGWNYSTGFITEDVLREHVPEGLEGESLALACGPPPMIQFALQPNLEKMGYNVKEDLLIF 907          
BLAST of NO14G02460 vs. NCBI_GenBank
Match: ABW05098.1 (nitrate reductase [Beta vulgaris] >ACB73000.1 nitrate reductase [Beta vulgaris])

HSP 1 Score: 792.7 bits (2046), Expect = 1.100e-225
Identity = 397/842 (47.15%), Postives = 542/842 (64.37%), Query Frame = 0
Query:   54 TSTDARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITPAAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELV-ALPNRTLPVTLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPDALEPGQYWVNFDGPDGELPKG---IYGTSIPLLKALDPAQDVLVAFKQNHERLLPDHGFPVRLIIPGYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSVDQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIPLKKGT----YKLQGYAYCGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDILRLVGCTNVACRAWDNSQNTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQPVVRIKHPAPIA--PGGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIAQRKDL-------SVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMNAGKDATEDFEAIHSKRAWAMLDEYLVGTL---GASLTSSSP--------EASAIAAPKEAA----VALQGKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLGLPVGMHIGLRAVINGESTKRQYTPVSDGDAKGHVELLVKVYRANQHPRFPDGGLMSQHLDRMSLGDCIDIDGPLGHITYEGPGCIRQLGEDVHVKHFVAVAGGTGITPVVQVLRAVLENPCDTTRFSLIYAARVPEDLLLREELDAWAEQY-EQFTVHYTVDVPPPDWPYSVGFLTAEMLAANFPEATKDMGALICGPPPMVNFAVKPNLEKLGY-TEDQFFIF 862
            +S DARD+ TAD W++R   MIRLTG+HPFN+EP    L+  GFITP  +HYVRNHGPVP   W+D  + VTGL V  P   ++D+LV    +R  PVTLVCAGNRRKE N+ +QS GF+WGS AVSTS+W GVPL  +L+ CG+     + G   V F+G + +LP G    YGTS+    ALDPA+D+++A+ QN E+L PDHGFPVR+IIPG+IGGRM+KWL RI ++  ES ++YHF DNRVLPS VD E A+ E WW KPE+IIN+LN+NS IT P H+E +P+   T    Y ++GYAY GGGR+V R+E++LD G++W + +L  +E P+++G++WCW  W+L+V++L L+G   +A RAWD   NTQP  L WNV+GMMNN WFR+ T V    + +  +  +HP       GGWM                  +   H+E    P++ +            + +  E+ +HNS    WI V G VYD T +L++HPGG  +I++NAG D TE+F+AIHS +A  +L+++ VG +   G +  SSSP          S +A  KEAA    VAL  + R I CKL+ K   S DV R RFGLP  D  LGLPVG HI L A ++ +   R YTP S  D  G+ +L+VK+Y   QHP+FP+GG+MSQHLD + LG  +++ GPLGHI Y G G     G+  + K    +AGGTGITP+ QV++A+L++P D T  +++YA R  +D+LLREELD WA+++ E+  V Y V+  P  W Y  GF++ ++L  + PE   D+ AL CGPPPM+ FAV+PNL+K+GY  +DQ  IF
Sbjct:   87 SSVDARDEGTADNWIERNSSMIRLTGKHPFNSEPPLTRLMHHGFITPVPLHYVRNHGPVPNAKWEDWTVEVTGL-VKRPARFTIDQLVNEFQSREFPVTLVCAGNRRKEQNMVKQSIGFNWGSAAVSTSVWRGVPLRDVLKRCGILTS--KKGALNVCFEGAE-DLPGGGGSKYGTSVRRELALDPARDIMLAYMQNGEKLSPDHGFPVRMIIPGFIGGRMVKWLKRIIVTTSESDNYYHFKDNRVLPSHVDAELANTEAWWYKPEFIINELNINSVITTPCHEEILPINAWTTQRPYTIRGYAYSGGGRKVTRVELTLDGGETWHVCELDIQEKPSKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDEGLNTQPEKLIWNVMGMMNNCWFRVKTNVCKPHKGEIGIVFEHPTQPGNKSGGWM------------------ARERHLEISDAPTLKRTASTPFMNTTSKMYSMSEVKKHNSAESAWIVVHGHVYDATRFLKDHPGGSDSILINAGTDCTEEFDAIHSDKAKRLLEDFRVGEIISTGYTSDSSSPGNSVHGGSNYSFLAPIKEAAPLRNVALNPRER-ISCKLIEKVSLSHDVRRFRFGLPSKDHVLGLPVGKHIFLCANVDDKLCMRAYTPSSTVDEVGYFDLVVKIYFKGQHPKFPNGGIMSQHLDSLELGSIVEVKGPLGHIEYLGKGNFTVHGKAKYAKKLAMIAGGTGITPIYQVMQAILKDPEDETEMNVVYANRTEDDILLREELDNWADEFRERVKVWYVVEKGPEGWRYDTGFISEKILREHIPEVGDDVLALTCGPPPMIQFAVQPNLDKMGYDIKDQLLIF 905          
BLAST of NO14G02460 vs. NCBI_GenBank
Match: XP_010670929.1 (PREDICTED: nitrate reductase [NADH] [Beta vulgaris subsp. vulgaris] >KMT20017.1 hypothetical protein BVRB_1g000370 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 792.0 bits (2044), Expect = 1.900e-225
Identity = 397/842 (47.15%), Postives = 542/842 (64.37%), Query Frame = 0
Query:   54 TSTDARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITPAAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELV-ALPNRTLPVTLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPDALEPGQYWVNFDGPDGELPKG---IYGTSIPLLKALDPAQDVLVAFKQNHERLLPDHGFPVRLIIPGYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSVDQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIPLKKGT----YKLQGYAYCGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDILRLVGCTNVACRAWDNSQNTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQPVVRIKHPAPIA--PGGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIAQRKDL-------SVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMNAGKDATEDFEAIHSKRAWAMLDEYLVGTL---GASLTSSSP--------EASAIAAPKEAA----VALQGKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLGLPVGMHIGLRAVINGESTKRQYTPVSDGDAKGHVELLVKVYRANQHPRFPDGGLMSQHLDRMSLGDCIDIDGPLGHITYEGPGCIRQLGEDVHVKHFVAVAGGTGITPVVQVLRAVLENPCDTTRFSLIYAARVPEDLLLREELDAWAEQY-EQFTVHYTVDVPPPDWPYSVGFLTAEMLAANFPEATKDMGALICGPPPMVNFAVKPNLEKLGY-TEDQFFIF 862
            +S DARD+ TAD W++R   MIRLTG+HPFN+EP    L+  GFITP  +HYVRNHGPVP   W+D  + VTGL V  P   ++D+LV    +R  PVTLVCAGNRRKE N+ +QS GF+WGS AVSTS+W GVPL  +L+ CG+     + G   V F+G + +LP G    YGTS+    ALDPA+D+++A+ QN E+L PDHGFPVR+IIPG+IGGRM+KWL RI ++  ES ++YHF DNRVLPS VD E A+ E WW KPE+IIN+LN+NS IT P H+E +P+   T    Y ++GYAY GGGR+V R+E++LD G++W + +L  +E P+++G++WCW  W+L+V++L L+G   +A RAWD   NTQP  L WNV+GMMNN WFR+ T V    + +  +  +HP       GGWM                  +   H+E    P++ +            + +  E+ +HNS    WI V G VYD T +L++HPGG  +I++NAG D TE+F+AIHS +A  +L+++ VG +   G +  SSSP          S +A  KEAA    VAL  + R I CKL+ K   S DV R RFGLP  D  LGLPVG HI L A ++ +   R YTP S  D  G+ +L+VK+Y   QHP+FP+GG+MSQHLD + LG  +++ GPLGHI Y G G     G+  + K    +AGGTGITP+ QV++A+L++P D T  +++YA R  +D+LLREELD WA+++ E+  V Y V+  P  W Y  GF++ ++L  + PE   D+ AL CGPPPM+ FAV+PNL+K+GY  +DQ  IF
Sbjct:   87 SSVDARDEGTADNWIERNSSMIRLTGKHPFNSEPPLTRLMHHGFITPVPLHYVRNHGPVPNAKWEDWTVEVTGL-VKRPARFTIDQLVNEFQSREFPVTLVCAGNRRKEQNMVKQSIGFNWGSAAVSTSVWRGVPLRDVLKRCGILTS--KKGALNVCFEGAE-DLPGGGGSKYGTSVRRELALDPARDIMLAYMQNGEKLSPDHGFPVRMIIPGFIGGRMVKWLKRIIVTTSESDNYYHFKDNRVLPSHVDAELANAEAWWYKPEFIINELNINSVITTPCHEEILPINAWTTQRPYTIRGYAYSGGGRKVTRVELTLDGGETWHVCELDIQEKPSKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDEGLNTQPEKLIWNVMGMMNNCWFRVKTNVCKPHKGEIGIVFEHPTQPGNKSGGWM------------------ARERHLEISDAPTLKRTASTPFMNTTSKMYSMSEVKKHNSAESAWIVVHGHVYDATRFLKDHPGGSDSILINAGTDCTEEFDAIHSDKAKRLLEDFRVGEIISTGYTSDSSSPGNSVHGGSNYSFLAPIKEAAPLRNVALNPRER-ISCKLIEKVSLSHDVRRFRFGLPSKDHVLGLPVGKHIFLCANVDDKLCMRAYTPSSTVDEVGYFDLVVKIYFKGQHPKFPNGGIMSQHLDSLELGSIVEVKGPLGHIEYLGKGNFTVHGKAKYAKKLAMIAGGTGITPIYQVMQAILKDPEDETEMNVVYANRTEDDILLREELDNWADEFRERVKVWYVVEKGPEGWRYDTGFISEKILREHIPEVGDDVLALTCGPPPMIQFAVQPNLDKMGYDIKDQLLIF 905          
The following BLAST results are available for this feature:
BLAST of NO14G02460 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes)
Total hits: 20
Match NameE-valueIdentityDescription
AET85052.10.000e+094.17nitrate reductase [Nannochloropsis sp. W2J3B][more]
EWM20573.18.500e-26671.36nitrate reductase [Nannochloropsis gaditana][more]
XP_020202176.11.200e-22748.91inducible nitrate reductase [NADH] 2 [Cajanus caja... [more]
PRW21069.11.300e-22647.65nitrate reductase [Chlorella sorokiniana][more]
XP_024018107.12.300e-22648.32nitrate reductase [NADH] [Morus notabilis][more]
XP_019416847.15.000e-22647.98PREDICTED: inducible nitrate reductase [NADH] 2-li... [more]
ADV03138.16.600e-22646.98nitrate reductase 1 [Medicago truncatula][more]
XP_013708150.18.600e-22648.13nitrate reductase [NADH], clone PBNBR1412-like [Br... [more]
ABW05098.11.100e-22547.15nitrate reductase [Beta vulgaris] >ACB73000.1 nitr... [more]
XP_010670929.11.900e-22547.15PREDICTED: nitrate reductase [NADH] [Beta vulgaris... [more]

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Relationships

This gene is member of the following syntenic_region feature(s):

Feature NameUnique NameSpeciesType
nonsL138nonsL138Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ncniR069ncniR069Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ngnoR023ngnoR023Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region


The following polypeptide feature(s) derives from this gene:

Feature NameUnique NameSpeciesType
NO14G02460.1NO14G02460.1-proteinNannochloropsis oceanica (N. oceanica IMET1)polypeptide


The following gene feature(s) are orthologous to this gene:

Feature NameUnique NameSpeciesType
jgi.p|Nanoce1779_2|590448gene_5828Nannochloropsis oceanica (N. oceanica CCMP1779)gene


The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
NO14G02460.1NO14G02460.1Nannochloropsis oceanica (N. oceanica IMET1)mRNA


Sequences
The following sequences are available for this feature:

gene sequence

>NO14G02460 ID=NO14G02460|Name=NO14G02460|organism=Nannochloropsis oceanica|type=gene|length=4186bp
ATGGAAACATACTTTTTTTGTTGCGCCACTTCTCGGCCGTACCCCACAAG
ACGTATCCACGATTGCGAAAGgtaaagttctatttcatgtcccctggtcg
actggtttgtgtaactccttgccaaaaacctacctgttactttatcaccg
acggcgttggcacaacaggcactgcaccaaaaaaccgccgcctcgattgg
gggccgcgaacatgacgtgtcggaacaggaaaagcaaccgctgcaaggcg
ccggatcttagcgcgcagtcgtagcagcaggaatcgacaatatgtgttct
cgtttctgcatacgtcgactctggacagctatgactgacccgactctgac
cacacttcatgacccacccaccacacaacgagggcagGTGCCGCAGCAGC
TTTAGCACGTTGCTCCTCTACATGGCCTTTAAACTCTCACCACAGGTGCC
TACGGCGCCTGAGCCGGTCCGTGCCACCAGTACAGACGCGCGAGACGATG
ACACGGCAGACAAGTGGGTTCAGCGCCTGCCAGGCATGATCCGCCTGACG
GGTCGCCACCCGTTTAACGCCGAGCCGCACACCAAGGAGCTGGTCGACGC
TGGCTTCATTACCCCCGCCGCCATGCACTATGTGCGgtaagtccttcttt
tctgggtcggccaatccagttcgtgtctctcatcatctttctaaactaca
ccatcacctacagCAACCACGGCCCAGTACCCAAGCTTGCCTGGGATGAC
CACCGCATAACCGTGACCGGCCTTGGCGTAGCTGAGCCCCAGGTTTTGTC
AATGGACGAACTGGTCGCCCTGCCCAATCGAACCCTGCCCGTCACTCTTG
TCTGCGCCGGCAATCGCCGCAAGGAGGTTAACGTCACCCGGCAGAGCAAG
GGCTTCAGCTGGGGCTCCGGTGCAGTGAGCACCTCCATTTGGACGGGCGT
GCCCCTGCACGTGCTTCTGCGCCACTGTGGCGTTGACCCCGATGCGTTAG
AGCCCGGACAATACTGGGTCAACTTCGACGGGCCTGACGGGGAGCTGCCC
AAGGGCATTTATGGCACGAGTATCCCCCTCCTCAAGgtaagcattcgggc
atatatttatgcatgcttgtgctcattgtcatccagttatgacaaaacta
tccatcttttcttttttccagGCGCTGGATCCAGCACAGGATGTGCTTGT
GGCCTTCAAGCAGAACCACGAGCGCCTCCTCCCCGACCACGGCTTCCCTG
TCCGCCTCATTATTCCAGgtacacaatcaaacatacacatacacacccat
acacgcacatacatacatgaaccacagccatattactcactttccttttt
atctcctctacttgcagGGTACATAGGGGGACGGATGATCAAATGGCTTA
CTCGCATCACTATCAGCCGCCAAGAGTCGCAGTCCTTTTACCACTTCCAC
GACAATCGCGTCCTGCCCTCGTCGGTGGACCAAGAGCGCGCGGATAATGA
GGGCTGGTGGCGCAAGCCTGAGTACATCATCAACGACCTCAACCTTAACT
CGGCCATCACCCATCCGgtacgtactcgtgtccgcgggcctcagctggga
ttacgaaagtttacaaacgtgcaagctcgccgcttatactgctgttcgtc
attttccctgccagACTCACGACGAGGAGATCCCGCTGAAGAAAGGCACT
TACAAACTCCAAGGCTACGCCTACTGTGGTGGCGGCCGGCAAGTGCAACG
CATGGAGGTCTCCCTCGACGATGGCAAGgtaggaagtatcaccttgtcgc
ggttttcacactgctatcattgactcaatcgacatttacacacccattcc
gactacgcaccacgagtagAGCTGGGAACTAGCTCAGCTGAGCAGTGAGG
AGTACCCAACTGAACACGGCCGCTTCTGGTGCTGGCGTATTTGGAATCTT
GATGTCGACATCCTACGTCTGgtgagtgcagcttgagaacgagcgagaaa
cgtctgtgttccatcacgctctcatatatacaatccctccctctctcata
gGTGGGCTGTACGAATGTCGCCTGTCGTGCATGGGACAACTCGCAGAACA
CACAGCCTCGAGACTTGACGTGGAACGTCCTAGGCAgtgagtatttcgtt
tcctctcttctagagatacttttattccatccatctctatctttacctgt
gtctatgagagtaagagtaatcgtcacaccttattcatacccctgaactc
ccctttccacccctcccttccctacccacagTGATGAACAACAGCTGGTT
CCGGCTTACAACCGCGGTTAGTCTCAACGATCGCCAGCAGCCTGTCGTCC
GCATCAAGCACCCGGCGCCCATTGCTCCTGGTGGGTGGATGGAGGCAGGG
GCCGACGAGACTGTAAATGTACAGGCCAAGACAACGGGTACCGGTAGCGG
ACGGTCACACGTGGAAGACAAGTCTGTCCCATCGATAGCGCAGCGTAAGG
ATTTGTCCGTCATCACGCGCGAAGAGTTGGCGCGGCACAACAGCAAgtac
gtgcggggggaagagggcatgaggaaggtgggtggagggagggcaacgac
gatgtttgtccatcaatacgtgtatatgacgcacagtcaaccgctgacta
aacctactgcacgaaaatcacagAACTGACTGCTGGATCGCTGTCAAGGG
TCAGGTCTACGATGTGACCCCCTACTTGCAGGAGCACCCGGGCGGCGTGG
CCGCCATCGTCATGAACGCCGGCAAAGACGCCACCGAGGATTTTGAGgta
cgcaacatatgaatatgcaagcccgccccgcatgcatcgatgagagcacg
gcacttaattgtgcccaccaaccacatacacacgcagGCGATCCACTCCA
AAAGGGCCTGGGCTATGCTGGATGAGTATCTGGTCGGCACCCTCGGGGCT
TCTTTGACCTCCTCCTCCCCTGAAGCCTCCGCCATCGCCGCGCCCAAGGA
GGCTGCCGTGGCGCTGCAAGGCAAGAACCGCGCCATCAAGTGCAAGCTCG
TGTTCAAGGAGTACGAGAGTCCCGACGTCCTGCGTATCCGATTTGGCCTC
CCGCAGCCAGACCAGCCCCTGGGCCTCCCTGTTGGGATGCATATCGGCCT
GCGCGCCGTGATCAACGGTGAGAGTACCAAGCGGCAATACACGCCCGTGT
CGGACGGGGACGCCAAGGGTCACGTGGAGCTGCTGGTCAAGgtacgtgcg
tgcaggcaaatgggttttgagtgattggacgatggagcctctctcatcct
ctgcgcgagctaggaccatctgacattgccagtccccgtcgccccttgcg
gacctgtagaccccgtagccccccttgcacgcacagcgccttatttcttg
acacacatgcacaccctaccacacagGTCTACCGCGCCAACCAGCACCCG
CGCTTTCCCGACGGCGGGCTTATGTCGCAGCACCTAGACCGTATGTCCCT
CGGCGACTGCATAGATATTGACGGACCCCTCGGTCACATTACTTACGAGG
GCCCCGGCTGCATTCGCCAACTGGGGGAGGACGTGCATGTCAAGCACTTT
GTGGCGGTCGCCGGCGGCACGGGCATCACGCCAGTCGTGCAGgtacgttg
acaatcgacgtcataaattttgaggaaaaaaggtgttggtgtcgggaata
cgtcgctagtgactgattcgaatccactcaatcaactctcaccgcagGTG
CTTCGTGCCGTGTTAGAGAATCCTTGCGACACTACCCGCTTTTCCCTCAT
CTATGCGGCCCGGGTTCCAGAGGATTTGCTCCTGCGCGAGGAGCTGGACG
CCTGGGCGGAGCAGTACGAACAGTTTACGgtaaggaatagtgttctcgac
atttggtctcagcttccgctcattttcttcttgacacgactcactaactc
aacattctgctttacttatctcttaatttaagGTGCACTACACCGTCGAT
GTTCCTCCCCCTGATTGGCCGTACTCCGTCGGTTTCCTCACGGCCGAGAT
GCTGGCGGCGAATTTCCCCGAGGCCACCAAGGACATGGGCGCGCTTATCT
GCGGCCCGCCGCCGATGGTGAACTTCGCCGTGAAACCCAACCTAGAAAAG
CTTGGTTACACCGAGGACCAGTTTTTCATCTTTTAA
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protein sequence of NO14G02460.1

>NO14G02460.1-protein ID=NO14G02460.1-protein|Name=NO14G02460.1|organism=Nannochloropsis oceanica|type=polypeptide|length=862bp
METYFFCCATSRPYPTRRIHDCERCRSSFSTLLLYMAFKLSPQVPTAPEP
VRATSTDARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITP
AAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELVALPNRTLPV
TLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPD
ALEPGQYWVNFDGPDGELPKGIYGTSIPLLKALDPAQDVLVAFKQNHERL
LPDHGFPVRLIIPGYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSV
DQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIPLKKGTYKLQGYAY
CGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDIL
RLVGCTNVACRAWDNSQNTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQ
PVVRIKHPAPIAPGGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIA
QRKDLSVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMN
AGKDATEDFEAIHSKRAWAMLDEYLVGTLGASLTSSSPEASAIAAPKEAA
VALQGKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLGLPVGMHIGLRA
VINGESTKRQYTPVSDGDAKGHVELLVKVYRANQHPRFPDGGLMSQHLDR
MSLGDCIDIDGPLGHITYEGPGCIRQLGEDVHVKHFVAVAGGTGITPVVQ
VLRAVLENPCDTTRFSLIYAARVPEDLLLREELDAWAEQYEQFTVHYTVD
VPPPDWPYSVGFLTAEMLAANFPEATKDMGALICGPPPMVNFAVKPNLEK
LGYTEDQFFIF*
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Synonyms
Publications
YearPublication
2016Wang Q, Lu Y, Xin Y, Wei L, Huang S, Xu J. Genome editing of model oleaginous microalgae Nannochloropsis spp. by CRISPR/Cas9. The Plant journal : for cell and molecular biology. 2016 12; 88(6):1071-1081.
2019Naduthodi MIS, Mohanraju P, Südfeld C, D'Adamo S, Barbosa MJ, van der Oost J. CRISPR-Cas ribonucleoprotein mediated homology-directed repair for efficient targeted genome editing in microalgae. Biotechnology for biofuels. 2019; 12:66.