CRISPR-Cas ribonucleoprotein mediated homology-directed repair for efficient targeted genome editing in microalgae

Overview
TitleCRISPR-Cas ribonucleoprotein mediated homology-directed repair for efficient targeted genome editing in microalgae
AuthorsNaduthodi MIS, Mohanraju P, Südfeld C, D'Adamo S, Barbosa MJ, van der Oost J
TypeJournal Article
Journal NameBiotechnology for biofuels
Volume12
IssueN/A
Year2019
Page(s)66
CitationNaduthodi MIS, Mohanraju P, Südfeld C, D'Adamo S, Barbosa MJ, van der Oost J. CRISPR-Cas ribonucleoprotein mediated homology-directed repair for efficient targeted genome editing in microalgae. Biotechnology for biofuels. 2019; 12:66.

Abstract

Background
Microalgae are considered as a sustainable feedstock for the production of biofuels and other value-added compounds. In particular,

Results
In this study, we report for the first time an alternative approach for CRISPR-Cas-mediated genome editing in

Conclusion
We show that the delivery of Cas RNP by electroporation can be an alternative approach to the presently reported plasmid-based Cas9 method for generating mutants of

Properties
Additional details for this publication include:
Property NameValue
Journal CountryEngland
Publication TypeJournal Article
Language Abbreng
LanguageEnglish
Publication ModelElectronic-eCollection
Elocation10.1186/s13068-019-1401-3
DOI10.1186/s13068-019-1401-3
Journal AbbreviationBiotechnol Biofuels
Publication Date2019
pISSN1754-6834
ISSN1754-6834
Cross References
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DatabaseAccession
PMID: PubMedPMID:30962821