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Overview
| Title | CRISPR-Cas ribonucleoprotein mediated homology-directed repair for efficient targeted genome editing in microalgae |
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| Authors | Naduthodi MIS, Mohanraju P, Südfeld C, D'Adamo S, Barbosa MJ, van der Oost J |
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| Type | Journal Article |
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| Journal Name | Biotechnology for biofuels |
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| Volume | 12 |
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| Issue | N/A |
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| Year | 2019 |
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| Page(s) | 66 |
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| Citation | Naduthodi MIS, Mohanraju P, Südfeld C, D'Adamo S, Barbosa MJ, van der Oost J. CRISPR-Cas ribonucleoprotein mediated homology-directed repair for efficient targeted genome editing in microalgae. Biotechnology for biofuels. 2019; 12:66. |
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Abstract
Background
Microalgae are considered as a sustainable feedstock for the production of biofuels and other value-added compounds. In particular, Results
In this study, we report for the first time an alternative approach for CRISPR-Cas-mediated genome editing in Conclusion
We show that the delivery of Cas RNP by electroporation can be an alternative approach to the presently reported plasmid-based Cas9 method for generating mutants of
Properties
Additional details for this publication include:
| Property Name | Value |
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| Journal Country | England |
| Publication Type | Journal Article |
| Language Abbr | eng |
| Language | English |
| Publication Model | Electronic-eCollection |
| Elocation | 10.1186/s13068-019-1401-3 |
| DOI | 10.1186/s13068-019-1401-3 |
| Journal Abbreviation | Biotechnol Biofuels |
| Publication Date | 2019 |
| pISSN | 1754-6834 |
| ISSN | 1754-6834 |
Cross References
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