NO14G02460, NO14G02460 (gene) Nannochloropsis oceanica | NanDeSyn: Nannochloropsis Design & Synthesis Initiative

Overview


Name	NO14G02460
Unique Name	NO14G02460
Type	gene
Organism	Nannochloropsis oceanica (N. oceanica IMET1)
Sequence length	4186
Alignment location	chr14:733236..737421 +

Link to JBrowse

Properties


Property Name	Value
Description	Nitrate reductase

Mutants

The following records are associated with this feature:


Title	Method	Accession date	Last modified date
Cas9 RNP mediated HDR for nitrate reductase	CRISPR/Cas	2019-05-10	2019-05-10
Deletion mutants for nitrate reductase	CRISPR/Cas	2019-05-10	2020-07-30

Expression

Analysis:

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Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
chr14	genome	chr14:733236..737421 +

Analyses

This gene is derived from or has results from the following analyses


Analysis Name	Date Performed
PRJNA769958	2024-08-13
GSE178672	2023-12-15
PRJNA933693	2023-10-26
PRJNA943449	2023-07-19
PXD016054	2021-01-14
PXD016699	2021-01-08
GSE149904	2020-10-10
NoIMET1_WT_HS	2020-09-29
PXD010030	2020-03-16
GSE139615	2020-03-11
PRJNA241382	2020-03-11
BLASTP analysis of N. oceanica IMET1 genes	2019-07-11
InterPro analysis for N. oceanica IMET1 genes	2019-07-11
GO annotation for IMET1v2 genes	2019-07-10
PRJNA182180	2019-04-25
Gene prediction for N. oceanica IMET1	2017-10-24

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0030151	molybdenum ion binding
GO:0016491	oxidoreductase activity
GO:0005488	binding
GO:0003824	catalytic activity

Vocabulary: **Biological Process**
Term	Definition
GO:0042128	nitrate assimilation
GO:0055114	oxidation-reduction process
GO:0009987	cellular process
GO:0008152	metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR036374	OxRdtase_Mopterin-bd_sf
IPR017938	Riboflavin_synthase-like_b-brl
IPR014756	Ig_E-set
IPR036400	Cyt_B5-like_heme/steroid_sf
IPR017927	Fd_Rdtase_FAD-bd
IPR005066	MoCF_OxRdtse_dimer
IPR000572	OxRdtase_Mopterin-bd_dom
IPR001433	OxRdtase_FAD/NAD-bd
IPR008333	OxRdtase_FAD-bd_dom
IPR001199	Cyt_B5-like_heme/steroid-bd
IPR001834	NADH-Cyt_B5_reductase
IPR008335	Mopterin_OxRdtase_euk
IPR001709	Flavoprot_Pyr_Nucl_cyt_Rdtase

Homology

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BLAST of NO14G02460 vs. NCBI_GenBank
Match: AET85052.1 (nitrate reductase [Nannochloropsis sp. W2J3B])

HSP 1 Score: 1695.6 bits (4390), Expect = 0.000e+0
Identity = 824/875 (94.17%), Postives = 825/875 (94.29%), Query Frame = 0

Query:   20 HDCERCRSSFSTLLLYMAFKLSPQVPTAPEPVRATSTDARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITPAAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELVALPNRTLPVTLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPDALEPGQYWVNFDGPDGELPKGIYGTSIPLLKALDPAQDVLVAFKQNHERLLPDHGFPVRLIIP---------------------------------GYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSVDQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIPLKKGTYKLQGYAYCGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDILRLVGCTNVACRAWDNSQNTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQPVVRIKHPAPIAPGGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIAQRKDLSVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMNAGKDATEDFEAIHSKRAWAMLDEYLVGTLGASLTSSSPEASAIAAPKEAAVALQGKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLGLPVGMHIGLRAVINGESTKRQYTPVSDGDAKGHVELLVKVYRANQHPRFPDGGLMSQHLDRMSLGDCIDIDGPLGHITYEGPGCIRQLGEDVHVKHFVAVAGGTGITPVVQVLRAVLENPCDTTRFSLIYAARVPEDLLLREELDAWAEQYEQFTVHYTVDVPPPDWPYSVGFLTAEMLAANFPEATKDMGALICGPPPMVNFAVKPNLEKLGYTEDQFFIF 862
            H+  RCRSSFSTLLLYMAFKLSPQVPTAPEPVRATSTDARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITPAAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELVALPNRTLPVTLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPDALEPGQYWVNFDGPDGELPKGIYGTSIPLLKALDPAQDVLVAFKQNHERLLPDHGFPVRLIIP                                 GYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSVDQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIPLKKGTYKLQGYAYCGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDILRL               NTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQPVVRIKHPAPIAPGGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIAQRKDLSVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMNAGKDATEDFEAIHSKRAWAMLDEYLVGTLGASLTSSSPEASAIAAPKEAAVALQGKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLGLPVGMHIGLRAVINGESTKRQYTPVSDGDAKGHVELLVKVYRANQHPRFPDGGLMSQHLDRMSLGDCIDIDGPLGHITYEGPGCIRQLGEDVHVKHFVAVAGGTGITPVVQVLRAVLENPCDTTRFSLIYAARVPEDLLLREELDAWAEQYEQFTVHYTVDVPPPDWPYSVGFLTAEMLAANFPEATKDMGALICGPPPMVNFAVKPNLEKLGYTEDQFFIF
Sbjct:    6 HNEGRCRSSFSTLLLYMAFKLSPQVPTAPEPVRATSTDARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITPAAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELVALPNRTLPVTLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPDALEPGQYWVNFDGPDGELPKGIYGTSIPLLKALDPAQDVLVAFKQNHERLLPDHGFPVRLIIPGTQSNIXXXXXXXXXXXEPQPYYSLSFLSPLLAGYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSVDQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIPLKKGTYKLQGYAYCGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDILRL---------------NTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQPVVRIKHPAPIAPGGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIAQRKDLSVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMNAGKDATEDFEAIHSKRAWAMLDEYLVGTLGASLTSSSPEASAIAAPKEAAVALQGKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLGLPVGMHIGLRAVINGESTKRQYTPVSDGDAKGHVELLVKVYRANQHPRFPDGGLMSQHLDRMSLGDCIDIDGPLGHITYEGPGCIRQLGEDVHVKHFVAVAGGTGITPVVQVLRAVLENPCDTTRFSLIYAARVPEDLLLREELDAWAEQYEQFTVHYTVDVPPPDWPYSVGFLTAEMLAANFPEATKDMGALICGPPPMVNFAVKPNLEKLGYTEDQFFIF 865

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BLAST of NO14G02460 vs. NCBI_GenBank
Match: EWM20573.1 (nitrate reductase [Nannochloropsis gaditana])

HSP 1 Score: 926.0 bits (2392), Expect = 8.500e-266
Identity = 436/611 (71.36%), Postives = 496/611 (81.18%), Query Frame = 0

Query:   36 MAFKLSPQVPTAPEPVRATSTDARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITPAAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELVALPNRTLPVTLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPDALEPGQYWVNFDGPDGELPKGIYGTSIPLLKALDPAQDVLVAFKQNHERLLPDHGFPVRLIIPGYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSVDQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIP-LKKGTYKLQGYAYCGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDILRLVGCTNVACRAWDNSQNTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQPVVRIKHPAPIAPGGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIAQRKDLSVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMNAGKDATEDFEAIHSKRAWAMLDEYLVGTLGASL--TSSSP----EASAIAAPKEAAVALQGKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLG 640
            MAF  SP +PT P P+  + TDA D+DT DKWV RLP MIRLTGRHPFNAEP TK LV+ GFITPAAMHYVRNHGPVP+L+W+ HRITV+G+GV +P   SMDEL++LP RTLPVTLVCAGNRRKEVNV R+SKGF+WG+GAVS S+WTGVPLHVLL  CG++   L PG YWVNFDGPDGELPKG+YGTSIPL KALDPAQDVLVA KQNHE L+PDHGFPVR+IIPGYIGGRMIKWLTRITIS + SQSFYH+HDNRVLPSSVDQERAD E WWRKPEYIINDLNLNSAITHP+H+EEIP L+ GTYK+QGYAYCGGGR+V R+EVSLDDG+SWEL ++S EEYPTEHGRFW WR+W+LDVD+LRL    +VACRAWD+SQNTQPRDLTWNVLGMMNNSWFRL T++  N +QQ V+ + HPAPI   GWME G   T     K  G   G  HVE+KS+P    RKDL  I+  ELARH S+TDCWIA+KG VY+VTPYL+EHPGGV AIVMN GKDATEDFEAIHS+ AW MLD+YLVG L  S   TSS P       A A+   A VALQGK + +KCKL+ KEY +PDVLR++F LP  +  LG
Sbjct:    1 MAFNTSPVLPTVPPPIHPSFTDAHDEDTVDKWVHRLPDMIRLTGRHPFNAEPDTKALVNQGFITPAAMHYVRNHGPVPQLSWESHRITVSGVGVPQPHTFSMDELLSLPQRTLPVTLVCAGNRRKEVNVHRKSKGFNWGAGAVSNSLWTGVPLHVLLEQCGLNARDLPPGSYWVNFDGPDGELPKGVYGTSIPLRKALDPAQDVLVAHKQNHEPLMPDHGFPVRMIIPGYIGGRMIKWLTRITISHEPSQSFYHYHDNRVLPSSVDQERADREDWWRKPEYIINDLNLNSAITHPSHNEEIPLLENGTYKIQGYAYCGGGRRVHRVEVSLDDGQSWELVEMSKEEYPTEHGRFWSWRLWHLDVDVLRLASGKSVACRAWDDSQNTQPRDLTWNVLGMMNNSWFRLKTSLHRNTQQQLVLHVHHPAPIGSLGWMEKGTGVTAMAPGKEQG---GAQHVEEKSIPVREIRKDLPAISSTELARHKSRTDCWIAIKGLVYNVTPYLKEHPGGVGAIVMNGGKDATEDFEAIHSRTAWRMLDKYLVGVLSPSSPGTSSPPSLRTSGGAPASIPAAKVALQGKGKKLKCKLLEKEYAAPDVLRLKFALPHGNATLG 608

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BLAST of NO14G02460 vs. NCBI_GenBank
Match: XP_020202176.1 (inducible nitrate reductase [NADH] 2 [Cajanus cajan])

HSP 1 Score: 799.3 bits (2063), Expect = 1.200e-227
Identity = 404/826 (48.91%), Postives = 536/826 (64.89%), Query Frame = 0

Query:   51 VRATSTDARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITPAAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELVA-LPNRTLPVTLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPDALEPGQYWVNFDGPDGELPKG---IYGTSIPLLKALDPAQDVLVAFKQNHERLLPDHGFPVRLIIPGYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSVDQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIPLKKGT----YKLQGYAYCGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDILRLVGCTNVACRAWDNSQNTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQPVVRIKHP--APIAPGGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIAQRKDLSVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMNAGKDATEDFEAIHSKRAWAMLDEYLVG---TLGASLTSSSP-----EASAIAAPKEA----AVALQGKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLGLPVGMHIGLRAVINGESTKRQYTPVSDGDAKGHVELLVKVYRANQHPRFPDGGLMSQHLDRMSLGDCIDIDGPLGHITYEGPGCIRQLGEDVHVKHFVAVAGGTGITPVVQVLRAVLENPCDTTRFSLIYAARVPEDLLLREELDAWAEQYEQFTVHYTVDVPPPD-WPYSVGFLTAEMLAANFPEATKDMGALICGPPPMVNFAVKPNLEKLGY 854
            V A+  D RDD T+D W++R   + RLTG+HPFN+EP    L+  GFITP  +HYVRNHGPVP+  W+D  + VTGL VA P   +MD+L+   P+R  P TLVCAGNRRKE N+ +QS GF+WG+ AVSTS+W GVPL  LL+ CG+   +   G   V F+G + +LP G    YGTSI    ALDP++D+++A+ QN ERL PDHGFPVR+IIPG+IGGRMIKWL RI ++ Q+SQS+YH+ DNRVLPS VD E A+ E WW KPEYIIN+LN+NS IT P HDE +P+   T    Y L+GYAY GGGR+V R+EV+LD G++W++  L   E P ++G++WCW  W+L+V++L L+G   +A RAWD + NTQP  L WNV+GMMNN WFR+ T V    + +  +  +HP  A    GGWM          + K     S  + +  KSV S          +  E+ +HNS    WI V G VYD T +L++HPGG  +I++NAG D TE+FEAIHS +A  ML++Y +G   T G +  SSSP     +A+ +A  KE     +VAL  + +   CKL+ K   S DV   RF LP  DQ LGLPVG H+ L A ++ +   R YTP S  D  G+ +L+VKVY    HP+FP+GGLMSQHLD + +G  +D+ GPLGHI Y G G     G+         +AGGTGITP+ QV +A+L++P D T+  ++YA R  +D+LLREELD WA+++++F V Y V     + W YSVGF+T  +L  + P A+ D  AL CGPPPM+ FAV+PNLEKLGY
Sbjct:   64 VEASVLDPRDDGTSDHWIERNASLFRLTGKHPFNSEPPLPRLMHHGFITPVPLHYVRNHGPVPRARWEDWTVEVTGL-VARPTRFTMDQLLRDFPSREFPATLVCAGNRRKEQNMVKQSIGFNWGAAAVSTSVWRGVPLRALLKRCGI--YSRTKGALHVCFEGAE-DLPGGGGSKYGTSIMREVALDPSRDIILAYMQNGERLTPDHGFPVRMIIPGFIGGRMIKWLKRIIVTTQQSQSYYHYQDNRVLPSHVDAELANAEAWWYKPEYIINELNINSVITTPCHDEILPINSWTTQIPYLLRGYAYSGGGRKVTRVEVTLDGGETWQVCTLDCPEKPNKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDEALNTQPEKLIWNVMGMMNNCWFRVKTNVCKAHKGEIGIVFEHPTQAGNQSGGWM---------AKEKHLEKSSESNPILKKSVSSPFMNTTSKTYSISEVRKHNSPDSAWIIVHGYVYDCTRFLKDHPGGTDSILINAGTDCTEEFEAIHSDKAKKMLEDYRIGELMTTGYTSDSSSPNTTVHDATHLAPIKEVTPQRSVALTPREKT-PCKLLSKTSLSHDVRLFRFSLPSEDQLLGLPVGKHLFLCATVDEKLCMRAYTPTSSVDEVGYFDLVVKVYFRGVHPKFPNGGLMSQHLDSLPIGSVLDVKGPLGHIEYTGRGNFLVHGKPRFATRLAMLAGGTGITPIYQVAQAILKDPEDRTQMHVVYANRTQDDILLREELDDWAQKHDRFKVWYVVQESLREGWEYSVGFITESILREHVPHASPDTLALTCGPPPMIQFAVQPNLEKLGY 875

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BLAST of NO14G02460 vs. NCBI_GenBank
Match: PRW21069.1 (nitrate reductase [Chlorella sorokiniana])

HSP 1 Score: 795.8 bits (2054), Expect = 1.300e-226
Identity = 395/829 (47.65%), Postives = 519/829 (62.61%), Query Frame = 0

Query:   54 TSTDARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITPAAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELVALPNRTLPVTLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPDALEPGQYWVNFDGPDGELPK---GIYGTSIPLLKALDPAQDVLVAFKQNHERLLPDHGFPVRLIIPGYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSVDQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIPLKKGTYKLQGYAYCGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDILRLVGCTNVACRAWDNSQNTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQPVVRIKHPAPIA--PGGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIAQRKDLSVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMNAGKDATEDFEAIHSKRAWAMLDEYLVGTLGASLTSSSPEASAIAAPKEAAVALQ----------------GKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLGLPVGMHIGLRAVINGESTKRQYTPVSDGDAKGHVELLVKVYRANQHPRFPDGGLMSQHLDRMSLGDCIDIDGPLGHITYEGPGCIRQLGEDVHVKHFVAVAGGTGITPVVQVLRAVLENPCDTTRFSLIYAARVPEDLLLREELDAWAEQYEQFTVHYTVDVPPPDWPYSVGFLTAEMLAANFPEATKDMGALICGPPPMVNFAVKPNLEKLGYTEDQFFIF 862
            T T   D+ T D W+ R P +IRLTGRHPFN EP    L ++ FITP ++HYVRNHG VP++ W++HR+TV GL V +P   +MDEL+ LP+  +  TL CAGNRRKE N+ +Q+ GF+WG    S S WTGV L  +LR CGV   ++E G ++V F GP GELPK   G YGTSI   KA+DPA DV++A+KQN   L PDHG+P+RLIIPGYIGGRMIKWL  I+++  ESQ+ YHFHDNRVLPS VD+  A+ EGWW  P++IINDLN+ SAI +P HDE +PL  GTY ++GYAYCG G ++ R E+SLDDGK+W LA ++    P  +G+ W W  W+++V I  L+ C  + CRAWD+S NTQP   TWN++GM++N  +R+       +     ++ +HP   A   GGWM    D+   V A  +  GSG         P+        + T  E+  HN+K   W  V G+VYD TP+L+EHPGG  +I++ AG DAT++F AIHS +A  ML +Y +G L       +PE    AAP                           + I  KLV KE  S +V R RF L  P    GLPVG H+ L A INGE   R YTP S  D +G+ EL+VK+Y ANQHPRFPDGG MSQ+L+ +++GDCI+  GP+GH+ Y G G     GE    +H   +AGGTGITP+ QV++AVL++  D+T+  L+YA   P+D+LLREELD  A  +  F+V YTVD     WP+S GF+  +M+      A  D  A++CGPPPM+ FA  PNLEKLGYT +Q   F
Sbjct:   51 TDTHPHDEGTKDAWIPRHPDLIRLTGRHPFNCEPTPARLFES-FITPPSLHYVRNHGAVPRIKWEEHRLTVNGL-VNKPATFTMDELLQLPSVDVTCTLTCAGNRRKEQNMVKQTIGFNWGPAGTSCSTWTGVRLADVLRLCGV--KSMEEGAHYVCFRGPKGELPKGDDGSYGTSITWGKAMDPASDVILAYKQNGRLLTPDHGYPLRLIIPGYIGGRMIKWLEEISVTEIESQNHYHFHDNRVLPSHVDEALANKEGWWYNPDFIINDLNVQSAIGYPAHDEVVPLTAGTYAVRGYAYCGNGNKIIRCELSLDDGKTWRLADVTHRTQPNTYGKHWAWVWWSIEVPIAELLTCPEIMCRAWDSSMNTQPNTFTWNLMGMLSNCCYRVKIHPRQTNTGGFALQFEHPTIAAGTVGGWMNRAEDQASTVAAPVS-AGSG---------PATPGSGSTQLFTMAEVEAHNTKESAWFVVDGKVYDATPFLKEHPGGADSILLVAGTDATDEFNAIHSAKAKKMLLDYHIGDL-------APEGVIPAAPAXXXXXXXXXXXXXXXXXXXXXXLDPRKRIPFKLVEKEELSHNVRRFRFALQSPQHRFGLPVGKHVFLYAKINGELVMRAYTPSSSDDERGYFELVVKIYFANQHPRFPDGGKMSQYLESLAIGDCIEAKGPIGHVEYLGRGRYSLDGEPHFARHISMIAGGTGITPMYQVIKAVLKDADDSTQLKLLYANVSPDDILLREELDQLAAAHPNFSVWYTVDNADEGWPFSTGFINEDMVRERLFPAGPDTIAVLCGPPPMLKFACLPNLEKLGYTPEQCIQF 858

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BLAST of NO14G02460 vs. NCBI_GenBank
Match: XP_024018107.1 (nitrate reductase [NADH] [Morus notabilis])

HSP 1 Score: 795.0 bits (2052), Expect = 2.300e-226
Identity = 403/834 (48.32%), Postives = 529/834 (63.43%), Query Frame = 0

Query:   57 DARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITPAAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELV-ALPNRTLPVTLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPDALEPGQYWVNFDGPDGELPKG---IYGTSIPLLKALDPAQDVLVAFKQNHERLLPDHGFPVRLIIPGYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSVDQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIPLKKGT----YKLQGYAYCGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDILRLVGCTNVACRAWDNSQNTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQPVVRIKHPAPIA--PGGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIAQRKDLSVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMNAGKDATEDFEAIHSKRAWAMLDEYLVG---TLGASLTSSSPEASA---------------IAAPKEAAVALQGKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLGLPVGMHIGLRAVINGESTKRQYTPVSDGDAKGHVELLVKVYRANQHPRFPDGGLMSQHLDRMSLGDCIDIDGPLGHITYEGPGCIRQLGEDVHVKHFVAVAGGTGITPVVQVLRAVLENPCDTTRFSLIYAARVPEDLLLREELDAWAEQYEQFTVHYTVDVPPPDWPYSVGFLTAEMLAANFPEATKDMGALICGPPPMVNFAVKPNLEKLGY-TEDQFFIF 862
            D RDD T+D W++R   M+RLTG+HPFN+EP    L+  GFITP  +HYVRNHG VP+ +WDD  + VTGL V  P   +MD+LV   P+R  PVTLVCAGNRRKE N+ +QS GF+WG+ AVSTS+WTGVPL  LL+ CG+ P   E G   V F+G + +LP G    YGTS+    A+DPA+D+++A+ QN ERL PDHGFPVR+IIPG+IGGRM+KWL RI ++ QES S+YH+ DNRVLPS VD E A+ E WW KPEYIIN+LN+NS IT P H E +P+   T    Y L+GYAY GGG++V R+EV++D G++W +  L   E P ++G++WCW  W+L+V++L L+    +A RAWD + NTQP  L WNV+GMMNN WFR+ T V    + +  +  +HP       GGWM A      + +AK+  T         KS  S          +  E+ +HNS    WI V G +YD T ++++HPGG  +I++NAG D TE+F+AIHS +A  +L++Y +G   T G +  SSSP  S                I  P    VAL  + + I CKLV K   S DV   RF LP   Q LGLPVG HI L AVING+   R YTP +  D  G+ EL+VKVY  N HP+FP+GG+MSQ+LD + LG  +D+ GPLG I Y G G     G+    K    +AGGTGITP+ QV++A+L++P D T   ++YA R  +D+LLREELD WA   E+F V Y V+     W YS GF+T  +L  + PE + D  AL CGPPPM+ FAV+PNLEK+ Y  +D   IF
Sbjct:   89 DPRDDGTSDSWIERNSTMVRLTGKHPFNSEPPLTRLMSHGFITPVPLHYVRNHGAVPRASWDDWTVEVTGL-VKRPAKFTMDQLVNDFPSREFPVTLVCAGNRRKEQNLVKQSIGFNWGAAAVSTSVWTGVPLRALLKRCGILP--REKGALNVCFEGAE-DLPGGGGSKYGTSVRKEIAMDPARDIILAYMQNRERLKPDHGFPVRMIIPGFIGGRMVKWLKRIVVTTQESDSYYHYKDNRVLPSHVDAELANAESWWYKPEYIINELNINSVITTPCHQEILPINSWTTQMPYTLKGYAYSGGGKKVARVEVTMDGGETWHVCTLDHPEKPNKYGKYWCWCFWSLEVEVLNLLVAKEIAVRAWDETINTQPEKLIWNVMGMMNNCWFRVKTNVCKPHKGEIGIIFEHPTQPGNQSGGWM-AKERHLESTEAKSNPT-------LKKSGSSPFMNTAAKTYSMSEVKKHNSPNSAWIIVHGHIYDCTRFIKDHPGGADSILINAGTDCTEEFDAIHSDKAKNLLEDYRIGELLTTGYASDSSSPNNSVHGSSNALSHLAPIKEITPPVSRPVALVPREK-IPCKLVKKTSISHDVRVFRFALPSEGQVLGLPVGKHIFLCAVINGKLCMRAYTPTTSIDEIGYFELVVKVYFKNVHPKFPNGGMMSQYLDSLPLGSALDVKGPLGDIEYMGRGQFSVHGKPKFAKRLAMLAGGTGITPIYQVVQAILKDPEDDTEMYVVYANRTEDDILLREELDEWARTCERFKVWYVVEKGGEGWQYSTGFVTESILREHIPEGSDDTLALACGPPPMIQFAVQPNLEKMNYDIKDSLLIF 909

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BLAST of NO14G02460 vs. NCBI_GenBank
Match: XP_019416847.1 (PREDICTED: inducible nitrate reductase [NADH] 2-like [Lupinus angustifolius])

HSP 1 Score: 793.9 bits (2049), Expect = 5.000e-226
Identity = 404/842 (47.98%), Postives = 543/842 (64.49%), Query Frame = 0

Query:   40 LSPQVPTAPEPVRATSTDARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITPAAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELV-ALPNRTLPVTLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPDALEPGQYWVNFDGPD---GELPKGIYGTSIPLLKALDPAQDVLVAFKQNHERLLPDHGFPVRLIIPGYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSVDQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIPLKKGT----YKLQGYAYCGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDILRLVGCTNVACRAWDNSQNTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQPVVRIKHPA-PIAP-GGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIAQRKDLSVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMNAGKDATEDFEAIHSKRAWAMLDEYLVGTL-----GASLTSSSP--------EASAIAAPKEAAVALQ----GKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLGLPVGMHIGLRAVINGESTKRQYTPVSDGDAKGHVELLVKVYRANQHPRFPDGGLMSQHLDRMSLGDCIDIDGPLGHITYEGPGCIRQLGEDVHVKHFVAVAGGTGITPVVQVLRAVLENPCDTTRFSLIYAARVPEDLLLREELDAWAEQYEQFTVHYTVDVPPPD-WPYSVGFLTAEMLAANFPEATKDMGALICGPPPMVNFAVKPNLEKLGY 854
            L+  V      +  +  D RDD T D W+QR   +IRLTG+HP+N+EP    L+  GFITP  +HYVRNHGPVP++ WDD  + VTGL V  P   +MD+L+   P+R LPVTLVCAGNRR E N+ +QS GF+WG+ AVSTS+W GVPL+ +L+ CG+       G  +V F+G D   G+     YGTSI    ALDP++D+++A+ QN ERL+PDHGFPVR+IIPG+IGGRM+KWL RI ++ Q+S S+YH+ DNRVLPS VD E A+ E WW KPEYIIN+LN+NS IT P HDE +P+   T    Y L+GYAY GGGR+V R+EV+LD G++W L+ L++EE P ++G++WCW +W+L+V++L ++G   +A RAWD + NTQP+ L WNV+GMMNN WFR+ + +    + +  +  +HP  P  P GGWM   A E    ++  T T    ++++ KSV S          +  ++ +H++    WI V GQVYD T +L+ HPGG  +I++NAG D TE+F+AIHS +A  ML+ Y +G L      +  ++SSP        E + +A  KE  V  Q         I CKLV K   S DV   RF LP   Q LGLPVG HI L A IN +   R YTP S  D  G+ +LLVK+Y  N HP+FP+GGLMSQHLD + +G  +D+ GPLGHI Y G G     GE    K    +AGGTGITP+ QV +A+L++P D T   +IYA R  +D+LL EELD W++ Y++F V Y V     + W Y+VGF+T  ++  + PEA+ D  AL CGPPPM+ FAV+PNLEKLGY
Sbjct:   55 LNDLVSKGTTEIETSIYDPRDDGTPDHWIQRNTTLIRLTGKHPYNSEPPLPRLMHHGFITPVPVHYVRNHGPVPRIRWDDWTMEVTGL-VNRPTQFTMDQLINNFPSRELPVTLVCAGNRRMEQNLVKQSIGFNWGAAAVSTSVWKGVPLYSVLKQCGIYNRT--KGALYVCFEGADDLPGDGGGTKYGTSIMREAALDPSRDIILAYMQNGERLIPDHGFPVRIIIPGFIGGRMVKWLKRIIVTTQQSDSYYHYRDNRVLPSHVDAELANAEAWWYKPEYIINELNINSVITTPCHDEILPINSWTTQRPYVLKGYAYSGGGRKVTRVEVTLDGGETWHLSTLNNEEKPNKYGKYWCWCLWSLEVEVLEILGAKEIAVRAWDEALNTQPQKLIWNVMGMMNNCWFRVKSNMCKPHKGEIGIVFEHPTQPGNPSGGWM---AKERHLEKSSYTST----TYLK-KSVSSPFMNTTSKTFSISQVNKHSNPDSAWIIVHGQVYDCTTFLKYHPGGKDSILINAGTDCTEEFDAIHSNKAKEMLESYRIGELVTTGYTSDSSTSSPNNTVHGNSEFTHLATIKEVTVPSQSIALNPREKIPCKLVSKTSISHDVRLFRFALPSNAQLLGLPVGKHIFLCATINDKLCMRAYTPTSAVDELGYFDLLVKIYFKNVHPKFPNGGLMSQHLDSLPVGSFLDVKGPLGHIQYTGKGNFLVHGEHKFAKRLAMLAGGTGITPIYQVAQAILKDPTDETEMHVIYANRTEDDILLMEELDDWSKNYDRFKVWYVVQESIREKWKYNVGFITESVIREHLPEASNDTLALTCGPPPMIQFAVQPNLEKLGY 885

[Close]

BLAST of NO14G02460 vs. NCBI_GenBank
Match: ADV03138.1 (nitrate reductase 1 [Medicago truncatula])

HSP 1 Score: 793.5 bits (2048), Expect = 6.600e-226
Identity = 389/828 (46.98%), Postives = 539/828 (65.10%), Query Frame = 0

Query:   57 DARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITPAAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELV-ALPNRTLPVTLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPDALEPGQYWVNFDGPDGELPKG---IYGTSIPLLKALDPAQDVLVAFKQNHERLLPDHGFPVRLIIPGYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSVDQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIPLKKGT----YKLQGYAYCGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDILRLVGCTNVACRAWDNSQNTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQPVVRIKHPAPIA--PGGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIAQRKDLSVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMNAGKDATEDFEAIHSKRAWAMLDEYLVGTLGASLTSSSPEAS--------------------AIAAPKEAAVALQGKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLGLPVGMHIGLRAVINGESTKRQYTPVSDGDAKGHVELLVKVYRANQHPRFPDGGLMSQHLDRMSLGDCIDIDGPLGHITYEGPGCIRQLGEDVHVKHFVAVAGGTGITPVVQVLRAVLENPCDTTRFSLIYAARVPEDLLLREELDAWAEQYEQFTVHYTVDVPPPD-WPYSVGFLTAEMLAANFPEATKDMGALICGPPPMVNFAVKPNLEKLGY 854
            D RD  T+D+W++R   M+RLTG+HPFN+EP  +ELV+ GFITP  +HYVRNHGPVPK  WD   I V+GL V  P   +M +L+   P+   PVTLVCAGNRRKE N+ +QS GF+WG+  +STS+W GVPL  +L+ CG+       G  +V F+G + ELP G    YGTSI    ALDP++D+++A+ QN + L PDHGFPVR+IIPG+IGGRM+KWL RI ++ ++S ++YH++DNRVLPS VD E A+ EGWW KPEYIIN+LN+NS IT P HDE +P+   T    Y L+GYAY GGGR+V R+EV++D G++W++  L   E P ++G++WCW  W+L+V++L L+G  ++A RAWD S NTQP++L WN++GMMNN WF++ +++   ++ +  +  +HP       GGWM          + +     S  + +  KS+ S          T  E+ +HN+    WI V G VYD T ++++HPGG  +I++NAG D TE+FEAIHS +A  ++++Y +G L  + + SSP+ S                     +   +  +VAL  + +   CKL+FK+  S DV   RF LP   Q LGLPVG HI L A I+ +   R YTP S  D KGH +L+VK+Y    HP+FP+GGLMSQHLD M +G  +DI GPLGHI Y G G     G+    K    +AGG+GITP+ QV++A+L++P D T   ++YA R  +D+LLREE+D+WA+++EQF V Y V     + W YSVGF+T  +L  + P+A+ D  AL+CGPPPMV FAVKPNLEKLGY
Sbjct:   80 DPRDAGTSDQWIKRNTSMLRLTGKHPFNSEPPLQELVEHGFITPVPLHYVRNHGPVPKAQWDSWTIEVSGL-VKNPTQFTMAQLIDDFPSHEFPVTLVCAGNRRKEQNMIKQSIGFNWGAAGISTSVWRGVPLCTVLKSCGI--LNRNSGALYVCFEGAE-ELPGGGGSKYGTSITREVALDPSRDIILAYMQNGDVLTPDHGFPVRIIIPGFIGGRMVKWLKRIIVTSKQSDNYYHYNDNRVLPSHVDAELANAEGWWYKPEYIINELNVNSVITSPCHDEILPINSCTTQMPYLLRGYAYSGGGRKVTRVEVTMDGGETWQVCTLDHSEKPNKYGKYWCWCFWSLEVEVLDLLGAKDIAVRAWDESLNTQPQNLIWNLMGMMNNCWFKVKSSLCKANKGEIGILFEHPTQPGNQSGGWM---------AKERQLEKSSDSNSILKKSLSSPFMITSSKTYTISEVEKHNNAESAWIIVHGHVYDCTRFIKDHPGGADSILINAGTDCTEEFEAIHSDKAKKIIEQYRIGELITTGSYSSPDISMHNTSVSVTTNLAPIKEITLPVTPLRSVSVALNPREKT-SCKLLFKKSISHDVRLFRFALPYEGQLLGLPVGKHIFLCATIDEKLCMRAYTPTSSVDEKGHFDLVVKIYLKGVHPKFPNGGLMSQHLDSMPIGSTLDIKGPLGHIEYAGRGNFMVHGKHKFAKRLAMLAGGSGITPIYQVVQAILKDPEDLTELHVVYANRSEDDILLREEMDSWAKKHEQFKVWYVVQESKREGWEYSVGFITESILKQHVPQASPDTLALVCGPPPMVQFAVKPNLEKLGY 893

[Close]

BLAST of NO14G02460 vs. NCBI_GenBank
Match: XP_013708150.1 (nitrate reductase [NADH], clone PBNBR1412-like [Brassica napus])

HSP 1 Score: 793.1 bits (2047), Expect = 8.600e-226
Identity = 400/831 (48.13%), Postives = 535/831 (64.38%), Query Frame = 0

Query:   57 DARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITPAAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELVA-LPNRTLPVTLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPDALEPGQYWVNFDGPDGELPKG---IYGTSIPLLKALDPAQDVLVAFKQNHERLLPDHGFPVRLIIPGYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSVDQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIPLKKGT----YKLQGYAYCGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDILRLVGCTNVACRAWDNSQNTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQPVVRIKHPAPIA--PGGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIAQRKDLSVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMNAGKDATEDFEAIHSKRAWAMLDEYLVGTLGASLTSSSPEASA---------IAAPKEAA----VALQGKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLGLPVGMHIGLRAVINGESTKRQYTPVSDGDAKGHVELLVKVYRANQHPRFPDGGLMSQHLDRMSLGDCIDIDGPLGHITYEGPGCIRQLGEDVHVKHFVAVAGGTGITPVVQVLRAVLENPCDTTRFSLIYAARVPEDLLLREELDAWA-EQYEQFTVHYTVDVPPPDWPYSVGFLTAEMLAANFPEATK-DMGALICGPPPMVNFAVKPNLEKLGY-TEDQFFIF 862
            D+RD+ TAD W+ R   M+RLTG+HPFNAE     L+  GFITP  +HYVRNHGPVPK  W +  I VTGL V  P +L+M++L++  P+R  PVTLVCAGNRRKE N+ +Q+ GF+WGS  VSTS+W GVPL  +LR CGV   +   G   V F+G + +LP G    YGTSI    A+DPA+D+++A+ QN E L PDHGFPVR+I+PG+IGGRM+KWL RI ++ QES S+YH+ DNRVLPS VD E A+ E WW KPEYIIN+LN+NS IT P H+E +P+   T    Y L+GYAY GGG++V R+EV+LD G++W + +L  +E P ++G+FWCW  W+LDV++L L+   +VA RAWD S NTQP  L WN++GMMNN WFR+ T V    R +  +  +HP       GGWM       ++ ++  T           KSV S        + +  ++ +HNS    WI V G VYD T +L++HPGG+ +I++NAG D TE+FEAIHS +A  +L++Y +G L  +   SSP  S          +A  KE A    +AL      +  KL+ K   S DV R RF LP  DQ LGLPVG HI L A IN +   R YTP S  DA GH++L+VKVY    HPRFP+GGLMSQHLD +S+G  +DI GPLGHI Y+G G     G+    K    +AGGTGITP+ QV++++L +P D T   ++YA R  +D+L+REEL+ WA +  E+  V Y V++    W YS GF+T ++L  + PE  + +  AL CGPPPM+ FA++PNLEK+GY  ++   IF
Sbjct:   90 DSRDESTADNWIHRNSSMVRLTGKHPFNAEAPLPRLMHHGFITPVPLHYVRNHGPVPKADWSEWTIEVTGL-VKRPVILTMEQLISEFPSREFPVTLVCAGNRRKEQNMVKQTIGFNWGSAGVSTSLWKGVPLSEILRRCGV--YSRRGGALNVCFEGAE-DLPGGGGAKYGTSIKKEMAMDPARDIILAYMQNGELLTPDHGFPVRIIVPGFIGGRMVKWLKRIIVTPQESDSYYHYKDNRVLPSLVDAELANAEAWWYKPEYIINELNINSVITTPGHEEILPINAFTTQKPYTLRGYAYSGGGKKVTRVEVTLDGGETWSVCELDHQEKPNKYGKFWCWCFWSLDVEVLDLLSAKDVAVRAWDESFNTQPDKLIWNLMGMMNNCWFRIRTNVCKPHRGEIGIVFEHPTRPGNQSGGWMAKERQLEISSESNPT---------LKKSVSSPFMNTSSKMYSISDVRKHNSVESAWIIVHGHVYDCTRFLKDHPGGIDSILINAGTDCTEEFEAIHSDKAKKILEDYRIGELITTGYDSSPNVSVHGGSNVAPLLAPIKELAPPKNIALVNPREKVPVKLIEKTSISHDVRRFRFALPSEDQQLGLPVGKHIFLCANINDKLCLRAYTPTSTVDAVGHIDLVVKVYFKQVHPRFPNGGLMSQHLDSLSIGAVLDIKGPLGHIEYKGKGNFMVNGKPKFAKKLAMLAGGTGITPIYQVIQSILSDPEDETEMYVVYANRTEDDILVREELEGWANKDKERLKVWYVVEIAKEGWNYSTGFITEDVLREHVPEGLEGESLALACGPPPMIQFALQPNLEKMGYNVKEDLLIF 907

[Close]

BLAST of NO14G02460 vs. NCBI_GenBank
Match: ABW05098.1 (nitrate reductase [Beta vulgaris] >ACB73000.1 nitrate reductase [Beta vulgaris])

HSP 1 Score: 792.7 bits (2046), Expect = 1.100e-225
Identity = 397/842 (47.15%), Postives = 542/842 (64.37%), Query Frame = 0

Query:   54 TSTDARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITPAAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELV-ALPNRTLPVTLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPDALEPGQYWVNFDGPDGELPKG---IYGTSIPLLKALDPAQDVLVAFKQNHERLLPDHGFPVRLIIPGYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSVDQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIPLKKGT----YKLQGYAYCGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDILRLVGCTNVACRAWDNSQNTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQPVVRIKHPAPIA--PGGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIAQRKDL-------SVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMNAGKDATEDFEAIHSKRAWAMLDEYLVGTL---GASLTSSSP--------EASAIAAPKEAA----VALQGKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLGLPVGMHIGLRAVINGESTKRQYTPVSDGDAKGHVELLVKVYRANQHPRFPDGGLMSQHLDRMSLGDCIDIDGPLGHITYEGPGCIRQLGEDVHVKHFVAVAGGTGITPVVQVLRAVLENPCDTTRFSLIYAARVPEDLLLREELDAWAEQY-EQFTVHYTVDVPPPDWPYSVGFLTAEMLAANFPEATKDMGALICGPPPMVNFAVKPNLEKLGY-TEDQFFIF 862
            +S DARD+ TAD W++R   MIRLTG+HPFN+EP    L+  GFITP  +HYVRNHGPVP   W+D  + VTGL V  P   ++D+LV    +R  PVTLVCAGNRRKE N+ +QS GF+WGS AVSTS+W GVPL  +L+ CG+     + G   V F+G + +LP G    YGTS+    ALDPA+D+++A+ QN E+L PDHGFPVR+IIPG+IGGRM+KWL RI ++  ES ++YHF DNRVLPS VD E A+ E WW KPE+IIN+LN+NS IT P H+E +P+   T    Y ++GYAY GGGR+V R+E++LD G++W + +L  +E P+++G++WCW  W+L+V++L L+G   +A RAWD   NTQP  L WNV+GMMNN WFR+ T V    + +  +  +HP       GGWM                  +   H+E    P++ +            + +  E+ +HNS    WI V G VYD T +L++HPGG  +I++NAG D TE+F+AIHS +A  +L+++ VG +   G +  SSSP          S +A  KEAA    VAL  + R I CKL+ K   S DV R RFGLP  D  LGLPVG HI L A ++ +   R YTP S  D  G+ +L+VK+Y   QHP+FP+GG+MSQHLD + LG  +++ GPLGHI Y G G     G+  + K    +AGGTGITP+ QV++A+L++P D T  +++YA R  +D+LLREELD WA+++ E+  V Y V+  P  W Y  GF++ ++L  + PE   D+ AL CGPPPM+ FAV+PNL+K+GY  +DQ  IF
Sbjct:   87 SSVDARDEGTADNWIERNSSMIRLTGKHPFNSEPPLTRLMHHGFITPVPLHYVRNHGPVPNAKWEDWTVEVTGL-VKRPARFTIDQLVNEFQSREFPVTLVCAGNRRKEQNMVKQSIGFNWGSAAVSTSVWRGVPLRDVLKRCGILTS--KKGALNVCFEGAE-DLPGGGGSKYGTSVRRELALDPARDIMLAYMQNGEKLSPDHGFPVRMIIPGFIGGRMVKWLKRIIVTTSESDNYYHFKDNRVLPSHVDAELANTEAWWYKPEFIINELNINSVITTPCHEEILPINAWTTQRPYTIRGYAYSGGGRKVTRVELTLDGGETWHVCELDIQEKPSKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDEGLNTQPEKLIWNVMGMMNNCWFRVKTNVCKPHKGEIGIVFEHPTQPGNKSGGWM------------------ARERHLEISDAPTLKRTASTPFMNTTSKMYSMSEVKKHNSAESAWIVVHGHVYDATRFLKDHPGGSDSILINAGTDCTEEFDAIHSDKAKRLLEDFRVGEIISTGYTSDSSSPGNSVHGGSNYSFLAPIKEAAPLRNVALNPRER-ISCKLIEKVSLSHDVRRFRFGLPSKDHVLGLPVGKHIFLCANVDDKLCMRAYTPSSTVDEVGYFDLVVKIYFKGQHPKFPNGGIMSQHLDSLELGSIVEVKGPLGHIEYLGKGNFTVHGKAKYAKKLAMIAGGTGITPIYQVMQAILKDPEDETEMNVVYANRTEDDILLREELDNWADEFRERVKVWYVVEKGPEGWRYDTGFISEKILREHIPEVGDDVLALTCGPPPMIQFAVQPNLDKMGYDIKDQLLIF 905

[Close]

BLAST of NO14G02460 vs. NCBI_GenBank
Match: XP_010670929.1 (PREDICTED: nitrate reductase [NADH] [Beta vulgaris subsp. vulgaris] >KMT20017.1 hypothetical protein BVRB_1g000370 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 792.0 bits (2044), Expect = 1.900e-225
Identity = 397/842 (47.15%), Postives = 542/842 (64.37%), Query Frame = 0

Query:   54 TSTDARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITPAAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELV-ALPNRTLPVTLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPDALEPGQYWVNFDGPDGELPKG---IYGTSIPLLKALDPAQDVLVAFKQNHERLLPDHGFPVRLIIPGYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSVDQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIPLKKGT----YKLQGYAYCGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDILRLVGCTNVACRAWDNSQNTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQPVVRIKHPAPIA--PGGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIAQRKDL-------SVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMNAGKDATEDFEAIHSKRAWAMLDEYLVGTL---GASLTSSSP--------EASAIAAPKEAA----VALQGKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLGLPVGMHIGLRAVINGESTKRQYTPVSDGDAKGHVELLVKVYRANQHPRFPDGGLMSQHLDRMSLGDCIDIDGPLGHITYEGPGCIRQLGEDVHVKHFVAVAGGTGITPVVQVLRAVLENPCDTTRFSLIYAARVPEDLLLREELDAWAEQY-EQFTVHYTVDVPPPDWPYSVGFLTAEMLAANFPEATKDMGALICGPPPMVNFAVKPNLEKLGY-TEDQFFIF 862
            +S DARD+ TAD W++R   MIRLTG+HPFN+EP    L+  GFITP  +HYVRNHGPVP   W+D  + VTGL V  P   ++D+LV    +R  PVTLVCAGNRRKE N+ +QS GF+WGS AVSTS+W GVPL  +L+ CG+     + G   V F+G + +LP G    YGTS+    ALDPA+D+++A+ QN E+L PDHGFPVR+IIPG+IGGRM+KWL RI ++  ES ++YHF DNRVLPS VD E A+ E WW KPE+IIN+LN+NS IT P H+E +P+   T    Y ++GYAY GGGR+V R+E++LD G++W + +L  +E P+++G++WCW  W+L+V++L L+G   +A RAWD   NTQP  L WNV+GMMNN WFR+ T V    + +  +  +HP       GGWM                  +   H+E    P++ +            + +  E+ +HNS    WI V G VYD T +L++HPGG  +I++NAG D TE+F+AIHS +A  +L+++ VG +   G +  SSSP          S +A  KEAA    VAL  + R I CKL+ K   S DV R RFGLP  D  LGLPVG HI L A ++ +   R YTP S  D  G+ +L+VK+Y   QHP+FP+GG+MSQHLD + LG  +++ GPLGHI Y G G     G+  + K    +AGGTGITP+ QV++A+L++P D T  +++YA R  +D+LLREELD WA+++ E+  V Y V+  P  W Y  GF++ ++L  + PE   D+ AL CGPPPM+ FAV+PNL+K+GY  +DQ  IF
Sbjct:   87 SSVDARDEGTADNWIERNSSMIRLTGKHPFNSEPPLTRLMHHGFITPVPLHYVRNHGPVPNAKWEDWTVEVTGL-VKRPARFTIDQLVNEFQSREFPVTLVCAGNRRKEQNMVKQSIGFNWGSAAVSTSVWRGVPLRDVLKRCGILTS--KKGALNVCFEGAE-DLPGGGGSKYGTSVRRELALDPARDIMLAYMQNGEKLSPDHGFPVRMIIPGFIGGRMVKWLKRIIVTTSESDNYYHFKDNRVLPSHVDAELANAEAWWYKPEFIINELNINSVITTPCHEEILPINAWTTQRPYTIRGYAYSGGGRKVTRVELTLDGGETWHVCELDIQEKPSKYGKYWCWCFWSLEVEVLDLLGAKEIAVRAWDEGLNTQPEKLIWNVMGMMNNCWFRVKTNVCKPHKGEIGIVFEHPTQPGNKSGGWM------------------ARERHLEISDAPTLKRTASTPFMNTTSKMYSMSEVKKHNSAESAWIVVHGHVYDATRFLKDHPGGSDSILINAGTDCTEEFDAIHSDKAKRLLEDFRVGEIISTGYTSDSSSPGNSVHGGSNYSFLAPIKEAAPLRNVALNPRER-ISCKLIEKVSLSHDVRRFRFGLPSKDHVLGLPVGKHIFLCANVDDKLCMRAYTPSSTVDEVGYFDLVVKIYFKGQHPKFPNGGIMSQHLDSLELGSIVEVKGPLGHIEYLGKGNFTVHGKAKYAKKLAMIAGGTGITPIYQVMQAILKDPEDETEMNVVYANRTEDDILLREELDNWADEFRERVKVWYVVEKGPEGWRYDTGFISEKILREHIPEVGDDVLALTCGPPPMIQFAVQPNLDKMGYDIKDQLLIF 905

The following BLAST results are available for this feature:

BLAST of NO14G02460 vs. NCBI_GenBank

BLAST of NO14G02460 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes)
Total hits: 20


Match Name	E-value	Identity	Description
AET85052.1	0.000e+0	94.17	nitrate reductase [Nannochloropsis sp. W2J3B]	[more]
EWM20573.1	8.500e-266	71.36	nitrate reductase [Nannochloropsis gaditana]	[more]
XP_020202176.1	1.200e-227	48.91	inducible nitrate reductase [NADH] 2 [Cajanus caja...	[more]
PRW21069.1	1.300e-226	47.65	nitrate reductase [Chlorella sorokiniana]	[more]
XP_024018107.1	2.300e-226	48.32	nitrate reductase [NADH] [Morus notabilis]	[more]
XP_019416847.1	5.000e-226	47.98	PREDICTED: inducible nitrate reductase [NADH] 2-li...	[more]
ADV03138.1	6.600e-226	46.98	nitrate reductase 1 [Medicago truncatula]	[more]
XP_013708150.1	8.600e-226	48.13	nitrate reductase [NADH], clone PBNBR1412-like [Br...	[more]
ABW05098.1	1.100e-225	47.15	nitrate reductase [Beta vulgaris] >ACB73000.1 nitr...	[more]
XP_010670929.1	1.900e-225	47.15	PREDICTED: nitrate reductase [NADH] [Beta vulgaris...	[more]

Pages

Relationships

This gene is member of the following syntenic_region feature(s):


Feature Name	Unique Name	Species	Type
nonsL138	nonsL138	Nannochloropsis oceanica (N. oceanica IMET1)	syntenic_region
ncniR069	ncniR069	Nannochloropsis oceanica (N. oceanica IMET1)	syntenic_region
ngnoR023	ngnoR023	Nannochloropsis oceanica (N. oceanica IMET1)	syntenic_region

The following polypeptide feature(s) derives from this gene:


Feature Name	Unique Name	Species	Type
NO14G02460.1	NO14G02460.1-protein	Nannochloropsis oceanica (N. oceanica IMET1)	polypeptide

The following gene feature(s) are orthologous to this gene:


Feature Name	Unique Name	Species	Type
jgi.p\|Nanoce1779_2\|590448	gene_5828	Nannochloropsis oceanica (N. oceanica CCMP1779)	gene

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Species	Type
NO14G02460.1	NO14G02460.1	Nannochloropsis oceanica (N. oceanica IMET1)	mRNA

Sequences

The following sequences are available for this feature:

gene sequence
protein sequence

gene sequence

>NO14G02460 ID=NO14G02460|Name=NO14G02460|organism=Nannochloropsis oceanica|type=gene|length=4186bp
ATGGAAACATACTTTTTTTGTTGCGCCACTTCTCGGCCGTACCCCACAAG
ACGTATCCACGATTGCGAAAGgtaaagttctatttcatgtcccctggtcg
actggtttgtgtaactccttgccaaaaacctacctgttactttatcaccg
acggcgttggcacaacaggcactgcaccaaaaaaccgccgcctcgattgg
gggccgcgaacatgacgtgtcggaacaggaaaagcaaccgctgcaaggcg
ccggatcttagcgcgcagtcgtagcagcaggaatcgacaatatgtgttct
cgtttctgcatacgtcgactctggacagctatgactgacccgactctgac
cacacttcatgacccacccaccacacaacgagggcagGTGCCGCAGCAGC
TTTAGCACGTTGCTCCTCTACATGGCCTTTAAACTCTCACCACAGGTGCC
TACGGCGCCTGAGCCGGTCCGTGCCACCAGTACAGACGCGCGAGACGATG
ACACGGCAGACAAGTGGGTTCAGCGCCTGCCAGGCATGATCCGCCTGACG
GGTCGCCACCCGTTTAACGCCGAGCCGCACACCAAGGAGCTGGTCGACGC
TGGCTTCATTACCCCCGCCGCCATGCACTATGTGCGgtaagtccttcttt
tctgggtcggccaatccagttcgtgtctctcatcatctttctaaactaca
ccatcacctacagCAACCACGGCCCAGTACCCAAGCTTGCCTGGGATGAC
CACCGCATAACCGTGACCGGCCTTGGCGTAGCTGAGCCCCAGGTTTTGTC
AATGGACGAACTGGTCGCCCTGCCCAATCGAACCCTGCCCGTCACTCTTG
TCTGCGCCGGCAATCGCCGCAAGGAGGTTAACGTCACCCGGCAGAGCAAG
GGCTTCAGCTGGGGCTCCGGTGCAGTGAGCACCTCCATTTGGACGGGCGT
GCCCCTGCACGTGCTTCTGCGCCACTGTGGCGTTGACCCCGATGCGTTAG
AGCCCGGACAATACTGGGTCAACTTCGACGGGCCTGACGGGGAGCTGCCC
AAGGGCATTTATGGCACGAGTATCCCCCTCCTCAAGgtaagcattcgggc
atatatttatgcatgcttgtgctcattgtcatccagttatgacaaaacta
tccatcttttcttttttccagGCGCTGGATCCAGCACAGGATGTGCTTGT
GGCCTTCAAGCAGAACCACGAGCGCCTCCTCCCCGACCACGGCTTCCCTG
TCCGCCTCATTATTCCAGgtacacaatcaaacatacacatacacacccat
acacgcacatacatacatgaaccacagccatattactcactttccttttt
atctcctctacttgcagGGTACATAGGGGGACGGATGATCAAATGGCTTA
CTCGCATCACTATCAGCCGCCAAGAGTCGCAGTCCTTTTACCACTTCCAC
GACAATCGCGTCCTGCCCTCGTCGGTGGACCAAGAGCGCGCGGATAATGA
GGGCTGGTGGCGCAAGCCTGAGTACATCATCAACGACCTCAACCTTAACT
CGGCCATCACCCATCCGgtacgtactcgtgtccgcgggcctcagctggga
ttacgaaagtttacaaacgtgcaagctcgccgcttatactgctgttcgtc
attttccctgccagACTCACGACGAGGAGATCCCGCTGAAGAAAGGCACT
TACAAACTCCAAGGCTACGCCTACTGTGGTGGCGGCCGGCAAGTGCAACG
CATGGAGGTCTCCCTCGACGATGGCAAGgtaggaagtatcaccttgtcgc
ggttttcacactgctatcattgactcaatcgacatttacacacccattcc
gactacgcaccacgagtagAGCTGGGAACTAGCTCAGCTGAGCAGTGAGG
AGTACCCAACTGAACACGGCCGCTTCTGGTGCTGGCGTATTTGGAATCTT
GATGTCGACATCCTACGTCTGgtgagtgcagcttgagaacgagcgagaaa
cgtctgtgttccatcacgctctcatatatacaatccctccctctctcata
gGTGGGCTGTACGAATGTCGCCTGTCGTGCATGGGACAACTCGCAGAACA
CACAGCCTCGAGACTTGACGTGGAACGTCCTAGGCAgtgagtatttcgtt
tcctctcttctagagatacttttattccatccatctctatctttacctgt
gtctatgagagtaagagtaatcgtcacaccttattcatacccctgaactc
ccctttccacccctcccttccctacccacagTGATGAACAACAGCTGGTT
CCGGCTTACAACCGCGGTTAGTCTCAACGATCGCCAGCAGCCTGTCGTCC
GCATCAAGCACCCGGCGCCCATTGCTCCTGGTGGGTGGATGGAGGCAGGG
GCCGACGAGACTGTAAATGTACAGGCCAAGACAACGGGTACCGGTAGCGG
ACGGTCACACGTGGAAGACAAGTCTGTCCCATCGATAGCGCAGCGTAAGG
ATTTGTCCGTCATCACGCGCGAAGAGTTGGCGCGGCACAACAGCAAgtac
gtgcggggggaagagggcatgaggaaggtgggtggagggagggcaacgac
gatgtttgtccatcaatacgtgtatatgacgcacagtcaaccgctgacta
aacctactgcacgaaaatcacagAACTGACTGCTGGATCGCTGTCAAGGG
TCAGGTCTACGATGTGACCCCCTACTTGCAGGAGCACCCGGGCGGCGTGG
CCGCCATCGTCATGAACGCCGGCAAAGACGCCACCGAGGATTTTGAGgta
cgcaacatatgaatatgcaagcccgccccgcatgcatcgatgagagcacg
gcacttaattgtgcccaccaaccacatacacacgcagGCGATCCACTCCA
AAAGGGCCTGGGCTATGCTGGATGAGTATCTGGTCGGCACCCTCGGGGCT
TCTTTGACCTCCTCCTCCCCTGAAGCCTCCGCCATCGCCGCGCCCAAGGA
GGCTGCCGTGGCGCTGCAAGGCAAGAACCGCGCCATCAAGTGCAAGCTCG
TGTTCAAGGAGTACGAGAGTCCCGACGTCCTGCGTATCCGATTTGGCCTC
CCGCAGCCAGACCAGCCCCTGGGCCTCCCTGTTGGGATGCATATCGGCCT
GCGCGCCGTGATCAACGGTGAGAGTACCAAGCGGCAATACACGCCCGTGT
CGGACGGGGACGCCAAGGGTCACGTGGAGCTGCTGGTCAAGgtacgtgcg
tgcaggcaaatgggttttgagtgattggacgatggagcctctctcatcct
ctgcgcgagctaggaccatctgacattgccagtccccgtcgccccttgcg
gacctgtagaccccgtagccccccttgcacgcacagcgccttatttcttg
acacacatgcacaccctaccacacagGTCTACCGCGCCAACCAGCACCCG
CGCTTTCCCGACGGCGGGCTTATGTCGCAGCACCTAGACCGTATGTCCCT
CGGCGACTGCATAGATATTGACGGACCCCTCGGTCACATTACTTACGAGG
GCCCCGGCTGCATTCGCCAACTGGGGGAGGACGTGCATGTCAAGCACTTT
GTGGCGGTCGCCGGCGGCACGGGCATCACGCCAGTCGTGCAGgtacgttg
acaatcgacgtcataaattttgaggaaaaaaggtgttggtgtcgggaata
cgtcgctagtgactgattcgaatccactcaatcaactctcaccgcagGTG
CTTCGTGCCGTGTTAGAGAATCCTTGCGACACTACCCGCTTTTCCCTCAT
CTATGCGGCCCGGGTTCCAGAGGATTTGCTCCTGCGCGAGGAGCTGGACG
CCTGGGCGGAGCAGTACGAACAGTTTACGgtaaggaatagtgttctcgac
atttggtctcagcttccgctcattttcttcttgacacgactcactaactc
aacattctgctttacttatctcttaatttaagGTGCACTACACCGTCGAT
GTTCCTCCCCCTGATTGGCCGTACTCCGTCGGTTTCCTCACGGCCGAGAT
GCTGGCGGCGAATTTCCCCGAGGCCACCAAGGACATGGGCGCGCTTATCT
GCGGCCCGCCGCCGATGGTGAACTTCGCCGTGAAACCCAACCTAGAAAAG
CTTGGTTACACCGAGGACCAGTTTTTCATCTTTTAA

protein sequence of NO14G02460.1

>NO14G02460.1-protein ID=NO14G02460.1-protein|Name=NO14G02460.1|organism=Nannochloropsis oceanica|type=polypeptide|length=862bp
METYFFCCATSRPYPTRRIHDCERCRSSFSTLLLYMAFKLSPQVPTAPEP
VRATSTDARDDDTADKWVQRLPGMIRLTGRHPFNAEPHTKELVDAGFITP
AAMHYVRNHGPVPKLAWDDHRITVTGLGVAEPQVLSMDELVALPNRTLPV
TLVCAGNRRKEVNVTRQSKGFSWGSGAVSTSIWTGVPLHVLLRHCGVDPD
ALEPGQYWVNFDGPDGELPKGIYGTSIPLLKALDPAQDVLVAFKQNHERL
LPDHGFPVRLIIPGYIGGRMIKWLTRITISRQESQSFYHFHDNRVLPSSV
DQERADNEGWWRKPEYIINDLNLNSAITHPTHDEEIPLKKGTYKLQGYAY
CGGGRQVQRMEVSLDDGKSWELAQLSSEEYPTEHGRFWCWRIWNLDVDIL
RLVGCTNVACRAWDNSQNTQPRDLTWNVLGMMNNSWFRLTTAVSLNDRQQ
PVVRIKHPAPIAPGGWMEAGADETVNVQAKTTGTGSGRSHVEDKSVPSIA
QRKDLSVITREELARHNSKTDCWIAVKGQVYDVTPYLQEHPGGVAAIVMN
AGKDATEDFEAIHSKRAWAMLDEYLVGTLGASLTSSSPEASAIAAPKEAA
VALQGKNRAIKCKLVFKEYESPDVLRIRFGLPQPDQPLGLPVGMHIGLRA
VINGESTKRQYTPVSDGDAKGHVELLVKVYRANQHPRFPDGGLMSQHLDR
MSLGDCIDIDGPLGHITYEGPGCIRQLGEDVHVKHFVAVAGGTGITPVVQ
VLRAVLENPCDTTRFSLIYAARVPEDLLLREELDAWAEQYEQFTVHYTVD
VPPPDWPYSVGFLTAEMLAANFPEATKDMGALICGPPPMVNFAVKPNLEK
LGYTEDQFFIF*

Synonyms

Publications


Year	Publication
2016	Wang Q, Lu Y, Xin Y, Wei L, Huang S, Xu J. Genome editing of model oleaginous microalgae Nannochloropsis spp. by CRISPR/Cas9. The Plant journal : for cell and molecular biology. 2016 12; 88(6):1071-1081.
2019	Naduthodi MIS, Mohanraju P, Südfeld C, D'Adamo S, Barbosa MJ, van der Oost J. CRISPR-Cas ribonucleoprotein mediated homology-directed repair for efficient targeted genome editing in microalgae. Biotechnology for biofuels. 2019; 12:66.