NO08G02150, NO08G02150 (gene) Nannochloropsis oceanica

BLAST of NO08G02150 vs. NCBI_GenBank
Match: EWM26750.1 (alanyl-trna synthetase [Nannochloropsis gaditana])

HSP 1 Score: 1635.2 bits (4233), Expect = 0.000e+0
Identity = 806/996 (80.92%), Postives = 881/996 (88.45%), Query Frame = 0

Query:    1 MSNDGTTEDVDDLRESVTDLRVETAWPARRVRQIFIDYFVQKRGHTYWSSSPVVPHDDPTLLFTNAGMNQFKPLFLGTADPKMPLSKLNRAVNSQKCIRAGGKHNDLEDVGKDTYHHTFFEMLGNWSFGSYFKEDAITWAWECLIDEFGLDPTRLYATYFGGDDKQGLKPDEEAKQLWLKFLPANRVLPFGCKDNFWEMGETGPCGPCSEIHYDRLSGREVPELVNMDSPDLIEIWNIVFIQYNREEGGELRELPNKHVDTGMGFERLTSIMQNRPSNYDTDIFLPLFDAIQELTGAAPYAGKLGKEDKESKDMAYRVVADHIRTLTFAISDGAVPSNDGRGYVLRRVLRRAVRYGQKVLGAPPGFFSKLVHVVVNQMKEVFPDLEEKEKRVMEVLAEEERAFNRTLDQGEKHFKKVAEELLLSAS--GSGNVGKVKFPAKEAHHLYSTMGFPIDLTELMAEEKGLTVDRAGFETLLAEDKRLSAMAAAAKKALGGKELVLEAEQTAWLQSRGVEMTDSSLKYVWHHEPSAKVLALYKGKGSTEDGVGFVDEVTAEDGIVAVILNTTSFYAEQGGQIYDTGVLTNLDGTDTLRVDSVQSYGGYVLHIGPLESGALRVGDTATCMVDYVRRANIAPNHTMTHVLNHALRRVLMKGVSDGSGLCDQKGSAVDEEKLRFDFAWNKALSTAELAEVETIVRERIKEELPVHSYIAPLEKALNIKALRQVFGERYPDPVRVLSVGQEVPPMLEDPANPAWEDKSVEFCGGTHLSNTRDAERFALIEETGIAKGIRRITAVTKDGAKAAEALAEEFAGLLAVAEGKPEDALEAEVKMLSMRLGPLSISAVEKERFRTGLEALVVRVKKYKKEKMAKNREKALGRVVEVATAAAAEGKGVVIERVDFGTDGKISKECLDKAAQVHPTGCFLLVSADEDTGKFGVYSSVPVKEASFDPVAWVAAAIEGAGGGRSGGKAPRGQGTGVGLENVEAALMAAKRFAGL 995
            MS  GT EDV+ L E V DL +ETAW ARRVRQ FIDYF  KR H +W SSPVVPHDDPTLLF NAGMNQ+KPLFLG  DPK+PLSKL RAVNSQKCIRAGGKHNDLEDVGKDTYHHTFFEMLGNWSFGSYFKE+AI WAW+CL++EFGLDP+RLYATYFGG++KQGL  DEEA+ +WL++LP NRVLPF CKDNFWEMGETGPCGPC+EIHYDRL  REVPELVNMDSPDLIEIWN+VFIQYNREEGG LRELP+KHVDTGMGFERLTSI+Q++PSNYDTDIF+PLFDAIQELTGA PYAGKLGK D ES+DMAYRVVADHIRTLTFAI+DGAVPSNDGRGYVLRR+LRRAVRYG KVLGA PGFFSKLVHVVVNQMK++FP+LEEKEKRV+EVLA+EERAFNRTLDQGEKHFKKVA+ELLL+AS  G     KV FPAKEAH+LYSTMGFP+DLTELMAEEKGLTVDRAGFETLLAEDKR SAMAAAAKK +GGKE+VLEAEQTAWLQSRGVE+TDSSLKYVWHHEPSA+V+ALYKGK      VGFV+EVTAEDG+VAVILNTTSFYAEQGGQI+DTGV+TNLDGTDTLRVDSVQSYGGYVLHIGPLESGALRVGDTATCMVDY RRA IAPNHTMTHVLN+ALRRVL+KGVSDGSGLCDQKGSAVD+EKLRFDFAWNKAL+  ELAEVETIVRERI +ELPVHSYIAPLEKAL+I +LRQVFGERYPDPVRVLSVG EVPPMLEDP NPAW+D SVEFCGGTHLSNTR+AERFALIEETGIAKGIRRITAVTKD A+AAE LAEEFAGLLA+AE KPE+ LE EVKML+ RLGPL+ISAVEKERFRT L+AL+ RVKK                                    DFGTDGKI+KE LDKA+Q+HP+GCFLL+SADED+GKFGVYS+ P   +  DP+AWVAAA+EGAGGGRSGGK+PRGQG G GLE + +A+ AAK FAG+
Sbjct:    1 MSIGGTAEDVNGLHEGVADLSIETAWSARRVRQTFIDYFTNKREHVFWPSSPVVPHDDPTLLFANAGMNQYKPLFLGIVDPKLPLSKLTRAVNSQKCIRAGGKHNDLEDVGKDTYHHTFFEMLGNWSFGSYFKEEAIAWAWDCLVNEFGLDPSRLYATYFGGNEKQGLTADEEARNIWLRYLPPNRVLPFDCKDNFWEMGETGPCGPCTEIHYDRLPAREVPELVNMDSPDLIEIWNVVFIQYNREEGGALRELPSKHVDTGMGFERLTSILQDKPSNYDTDIFMPLFDAIQELTGAPPYAGKLGKADPESRDMAYRVVADHIRTLTFAIADGAVPSNDGRGYVLRRILRRAVRYGAKVLGAAPGFFSKLVHVVVNQMKDIFPELEEKEKRVVEVLADEERAFNRTLDQGEKHFKKVADELLLAASQRGGDKGEKVAFPAKEAHYLYSTMGFPVDLTELMAEEKGLTVDRAGFETLLAEDKRQSAMAAAAKKEMGGKEIVLEAEQTAWLQSRGVEITDSSLKYVWHHEPSARVMALYKGK-----AVGFVEEVTAEDGVVAVILNTTSFYAEQGGQIHDTGVITNLDGTDTLRVDSVQSYGGYVLHIGPLESGALRVGDTATCMVDYERRARIAPNHTMTHVLNYALRRVLLKGVSDGSGLCDQKGSAVDDEKLRFDFAWNKALTKEELAEVETIVRERISQELPVHSYIAPLEKALHINSLRQVFGERYPDPVRVLSVGHEVPPMLEDPTNPAWQDTSVEFCGGTHLSNTREAERFALIEETGIAKGIRRITAVTKDMARAAEELAEEFAGLLALAEVKPEEELEGEVKMLNTRLGPLTISAVEKERFRTALDALLTRVKKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDFGTDGKIAKEALDKASQIHPSGCFLLISADEDSGKFGVYSTAPKLGSPLDPIAWVAAAVEGAGGGRSGGKSPRGQGVGEGLEKIGSAVTAAKAFAGV 991          

BLAST of NO08G02150 vs. NCBI_GenBank
Match: CBJ30506.1 (Alanyl-tRNA Synthetase [Ectocarpus siliculosus])

HSP 1 Score: 1094.0 bits (2828), Expect = 0.000e+0
Identity = 550/979 (56.18%), Postives = 694/979 (70.89%), Query Frame = 0

Query:   23 ETAWPARRVRQIFIDYFVQKRGHTYWSSSPVVPHDDPTLLFTNAGMNQFKPLFLGTADPKMPLSKLNRAVNSQKCIRAGGKHNDLEDVGKDTYHHTFFEMLGNWSFGSYFKEDAITWAWECLIDEFGLDPTRLYATYFGGDDKQGLKPDEEAKQLWLKFLPANRVLPFGCKDNFWEMGETGPCGPCSEIHYDRLSGREVPELVNMDSPDLIEIWNIVFIQYNREEGGELRELPNKHVDTGMGFERLTSIMQNRPSNYDTDIFLPLFDAIQELTGAAPYAGKLGKEDKESKDMAYRVVADHIRTLTFAISDGAVPSNDGRGYVLRRVLRRAVRYGQKVLGAPPGFFSKLVHVVVNQMKEVFPDLEEKEKRVMEVLAEEERAFNRTLDQGEKHFKKVAEELLLSASGSGNVGKVKFPAKEAHHLYSTMGFPIDLTELMAEEKGLTVDRAGFETLLAEDKRLSAMAAAAKKALGGKELVLEAEQTAWLQSRGVEMTDSSLKYVWHHEPSAKVLALYKGKGSTEDGVGFVDEVT-----AEDGIVAVILNTTSFYAEQGGQIYDTGVLTNLDGTDTLRVDSVQSYGGYVLHIGPLESGALRVGDTATCMVDYVRRANIAPNHTMTHVLNHALRRVLMKGVSDGSGLCDQKGSAVDEEKLRFDFAWNKALSTAELAEVETIVRERIKEELPVHSYIAPLEKALNIKALRQVFGERYPDPVRVLSVGQEVPPMLEDPANPAWEDKSVEFCGGTHLSNTRDAERFALIEETGIAKGIRRITAVTKDGAKAAEALAEEFAGLLAVAEGKPEDALEAEVKMLSMRLGPLSISAVEKERFRTGLEALVVRVKKYKKEKMAKNREKALGRVVEVATAAAAEGKGV----VIERVDFGTDGKISKECLDKAAQVHP-TGCFLLVSADEDTGKFGVYSSVP-VKEASFDPVAWVAAAIEGAG-GGRSGGKAPRGQGTGVGLENVEAALMAAK 990
            +  WPA RVR  FI +F +K  HT+W SSPVVPHDDPTLLF NAGMNQFKPLFLGTADP +PLSKL RA NSQKCIRAGGKHNDL+DVGKD YHHTFFEMLGNWSFGSYFKE+AI +AWECL   + LDP+RLYATYFGGD+K  +  DE+AK++WLK+LP +RVLPF   DNFWEMG+ GPCGPC EIHYDR+ GR+  + VNMD P++IEIWN+VFIQYNRE  G L+ELPNKH+DTG+GFERL SI+Q + SNYDTD+F+P+F+AI+  TGA PY GK+G+ED ++ DMAYRVVADHIRTLT AI+DG  PSNDGRGYVLRRVLRRA+RYGQ+VLG  PGFF  LV VVV  MK +FP+L + E RV  V+ +EE +FN+TLD+G K F KVA     +A G   V    FP   A  LYS+MGFP+DL  LMAEEKGL+VD  GF+  ++++           KA GGK ++LEAEQ  WL  R  E T++S +Y WH +P+AKVLAL+ GKG  EDGVGFV   +     A+   V V+L+ TSFYAE GGQIYD G L+  DG+ +  V  V S+G Y LHIG +++G L VG    C VDY RRAN+APNHTMTHVLN ALR+VL        G+CDQKGS +D+EKLRFDF+W+ +L+  ++A+VE +V ++I + LPV++++APLE+A  I++LR VFGERYPDPVRVLS+GQ+VP MLEDP N AW D SVEFCGGTHLSNT++AE F ++EE GIAKG+RRI AVT+  A AA   A+ F   L VA+G  + +L+  V+ L   L    IS   K + R  L  LV + K  KK+ M    E+ +G+ V V  A A + K      ++ R DFG D K + + L + ++  P     +L +ADE+  ++     +P  K A  D   WV  A++ +G GG+ GGK  R QG   G++ ++ A+  A+
Sbjct:   28 DNGWPASRVRDSFISFFTEKHDHTFWKSSPVVPHDDPTLLFANAGMNQFKPLFLGTADPALPLSKLTRATNSQKCIRAGGKHNDLDDVGKDLYHHTFFEMLGNWSFGSYFKEEAIGYAWECLTTVYKLDPSRLYATYFGGDEKLRVGADEDAKKIWLKYLPESRVLPFSLADNFWEMGDVGPCGPCVEIHYDRIGGRDAAKDVNMDKPEVIEIWNVVFIQYNRENDGSLKELPNKHIDTGLGFERLASILQGKMSNYDTDVFVPIFEAIRSATGARPYTGKVGEEDSDNMDMAYRVVADHIRTLTIAITDGCTPSNDGRGYVLRRVLRRAIRYGQQVLGGKPGFFHTLVPVVVGLMKGIFPELVDAEARVTAVIKDEEMSFNKTLDKGLKEFNKVA-----AALGDSKV----FPGTAAFQLYSSMGFPLDLLTLMAEEKGLSVDVEGFDQKMSDEXXXXXXXXXXXKAAGGKPMILEAEQMDWLSKRDTEATEASGRYTWHQQPAAKVLALFVGKGEGEDGVGFVPSASAVADEADQQTVGVVLDCTSFYAEGGGQIYDIGELSLSDGSGSFAVRQVSSFGAYTLHIGQVKAGTLSVGSDVVCKVDYERRANVAPNHTMTHVLNFALRKVL----DPSDGMCDQKGSFLDDEKLRFDFSWSGSLTAEQVADVEDLVNDKINKNLPVYAFVAPLEQAQRIQSLRGVFGERYPDPVRVLSIGQDVPAMLEDPENSAWSDVSVEFCGGTHLSNTQEAETFVILEEAGIAKGVRRIVAVTRGLALAAVERAKAFEQQLDVADGIEDISLDTTVRRLGAELDGAVISVKTKNKLRARLATLVQKAKALKKKVM----EERVGQAVTVGKAEAEKTKEAGGTQLVFRADFGCDAKSAVKILAEVSKACPELTSMMLFTADEEADRYLCCGLIPNSKAAKKDTNKWVQCALDASGRGGKGGGKGDRAQGISQGVDTIDKAMEVAR 989          

BLAST of NO08G02150 vs. NCBI_GenBank
Match: XP_002182439.1 (predicted protein [Phaeodactylum tricornutum CCAP 1055/1] >EEC46340.1 predicted protein [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 1032.7 bits (2669), Expect = 7.400e-298
Identity = 538/975 (55.18%), Postives = 672/975 (68.92%), Query Frame = 0

Query:   26 WPARRVRQIFIDYFVQKRGHTYWSSSPVVPHDDPTLLFTNAGMNQFKPLFLGTADPKMPLSKLNRAVNSQKCIRAGGKHNDLEDVGKDTYHHTFFEMLGNWSFGSYFKEDAITWAWECLIDEFGLDPTRLYATYFGGDDKQGLKP-DEEAKQLWLKFLPANRVLPFGCKDNFWEMGETGPCGPCSEIHYDRLSGREVPELVNMDSPDLIEIWNIVFIQYNREEGGELRELPNKHVDTGMGFERLTSIMQNRPSNYDTDIFLPLFDAIQELTGAAPYAGKLGKEDKESKDMAYRVVADHIRTLTFAISDGAVPSNDGRGYVLRRVLRRAVRYGQKVLGAPPGFFSKLVHVVVNQMKEVFPDLEEKEKRVMEVLAEEERAFNRTLDQGEKHFKKVAEELLLSASGSGNVGKVKFPAKEAHHLYSTMGFPIDLTELMAEEKGLTVDRAGFETLLAEDKRLSAMAAAAKKALG-GKELVLEAEQTAWLQSRGVEMTDSSLKYVWHHE-PSAKVLALYKGKGSTEDGVGFVDEVTAEDGIVAVILNTTSFYAEQGGQIYDTGVLTNLDGTDTLRVDSVQSYGGYVLHIGPLESGALRVGDTATCMVDYVRRANIAPNHTMTHVLNHALRRVLMKGVSDGSG-----LCDQKGSAVDEEKLRFDFAWNKALSTAELAEVETIVRERIKEELPVHSYIAPLEKALNIKALRQVFGERYPDPVRVLSVGQE-VPPMLEDPANPAWEDKSVEFCGGTHLSNTRDAERFALIEETGIAKGIRRITAVTKDGAKAAEALAEEFAGLLAVAEGKPEDALEAEVKMLSMRLGPLSISAVEKERFRTGLEALVVRVKKYKKEKMAKNREKALGRVVEVATAAAAEGKGVVIERVDFGTDGKISKECLDKAAQVHPTGCFLLVSADEDTGKFGVYSSVPVKEASFDPVAWVAAAIEGAGGGRSGGKAPRGQGTGVGLENVEAALMAAKRF 992
            WP  +VR  F +YFV++ GH +W SSP VP DDPTLLFTNAGMNQ+KPLFLGT DP + +SKL RAVNSQKCIRAGGKHNDL+DVGKD YHHTFFEMLGNWSFG YFK  AI  AW+CL   FGLDP RLYATYF GD+   L P DEEA+QLWL++LP +RVLPF  +DNFWEMG TGPCGPC+EIHYDR+ GR+  +LVN D PD++EIWN+VFIQYNRE  G LR LP +HVDTGMGFERLTSI+QN  SNYDTDIF+PLF AIQ +TGA PYA K+GKED E  DMAYRVVADHIRTL FAI+DGAVPSNDGRGYVLRRVLRRAVRYG++ LGA  GFF+KLV   V+ M   FP++ EK++ V  ++ EEE +F+RTLD+G + F ++AE++     G+  +    F   +AH LY++MGFP+DLTELMAEEKG+T+++  FE  + E+  +S  A  AK A G GK++ L AEQT++L  + +  TD + KYVWH E     V A + G+  TED +GFV  ++ E   V ++L+ +SFYAE GGQ+YD G LT+  G   +++ +VQ+YG +VLH+G + SG L VGDT  C VDYVRRA IA NHTMTHVLNHALR VL+K     SG       DQKGS VDE KLRFDF+W+  L+  +LAEVE +  +RI   +PV +Y+APL  A  I +LR VFGE+YPDPVRV++V    VP ML +P +  W + SVEFCGGTHL+NT++AE F L+ E GIAKGIRRITA+T   AK A ALA EF   L  AE    D LE+ VK LS  L  L I+AV+K +FR  L  +  +V  YKK+K+A                    G   V+ R DFG +GK++K  +    +       LLV+AD +  +F V +  P      +  AW+ AA +G    + GGK    Q    G+E V+  L  A++F
Sbjct:   15 WPMEKVRDTFKNYFVEQHGHVFWPSSPCVPVDDPTLLFTNAGMNQYKPLFLGTCDPNLEMSKLTRAVNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKAGAIDMAWQCLTVTFGLDPERLYATYFAGDE---LTPVDEEARQLWLRYLPDDRVLPFDARDNFWEMGATGPCGPCTEIHYDRIGGRDASKLVNADLPDVMEIWNVVFIQYNREADGSLRPLPAQHVDTGMGFERLTSILQNVDSNYDTDIFIPLFTAIQNITGARPYAKKVGKEDPEYIDMAYRVVADHIRTLCFAITDGAVPSNDGRGYVLRRVLRRAVRYGRQNLGAELGFFAKLVPAFVDVMGSAFPEVVEKQEYVTGIIQEEEESFSRTLDKGLQKFNELAEKV-----GADQI----FSGADAHFLYTSMGFPVDLTELMAEEKGMTLNKEEFEAKMQEEHDISQAAHLAKMAGGSGKDMRLVAEQTSYLVGQNISATDDAAKYVWHEELADCVVKACFIGRNETEDMIGFVGSISPESSAVGIVLDKSSFYAEAGGQVYDVGTLTSSTGA-VVKITNVQAYGQFVLHLGEVASGTLSVGDTVKCSVDYVRRAPIASNHTMTHVLNHALREVLIKRPEKESGKTSTLTVDQKGSLVDETKLRFDFSWSGQLTPEQLAEVEKLCMDRIVNAVPVDAYVAPLGDAQQISSLRAVFGEKYPDPVRVVAVSDHAVPEMLANPQDSQWNEYSVEFCGGTHLTNTKEAEAFVLLAEEGIAKGIRRITAITTGEAKKAIALANEFESKLTAAEVVQGDDLESTVKQLSAELDGLDIAAVKKMQFREQLATMTKQVLAYKKQKLA--------------------GGSKVVMRFDFGVEGKVAKSVMTAFGKQVKDKALLLVTADPEADRFMVIAGAPKAMKDLNCKAWIEAATDGL-DAKGGGKPDSAQYQVSGVEAVDTVLEKARKF 955          

BLAST of NO08G02150 vs. NCBI_GenBank
Match: GAX25155.1 (alanyl-tRNA synthetase [Fistulifera solaris])

HSP 1 Score: 1031.6 bits (2666), Expect = 1.700e-297
Identity = 555/970 (57.22%), Postives = 670/970 (69.07%), Query Frame = 0

Query:   23 ETAWPARRVRQIFIDYFVQKRGHTYWSSSPVVPHDDPTLLFTNAGMNQFKPLFLGTADPKMPLSKLNRAVNSQKCIRAGGKHNDLEDVGKDTYHHTFFEMLGNWSFGSYFKEDAITWAWECLIDEFGLDPTRLYATYFGGDDKQGLKPDEEAKQLWLKFLPANRVLPFGCKDNFWEMGETGPCGPCSEIHYDRLSGREVPELVNMDSPDLIEIWNIVFIQYNREEGGELRELPNKHVDTGMGFERLTSIMQNRPSNYDTDIFLPLFDAIQELTGAAPYAGKLGKEDKESKDMAYRVVADHIRTLTFAISDGAVPSNDGRGYVLRRVLRRAVRYGQKVLGAPPGFFSKLVHVVVNQMKEVFPDLEEKEKRVMEVLAEEERAFNRTLDQGEKHFKKVAEELLLSASGSGNVGKVKFPAKEAHHLYSTMGFPIDLTELMAEEKGLTVDRAGFETLLAEDKRLSAMAAAAK-KALGGKELVLEAEQTAWLQSRGVEMTDSSLKYVWH-HEPSAKVLALYKGKGSTEDGVGFVDEVTAEDGIVAVILNTTSFYAEQGGQIYDTGVLTNLDGTDTLRVDSVQSYGGYVLHIGPLESGALRVGDTATCMVDYVRRANIAPNHTMTHVLNHALRRVLMKGVSDGSGLCDQKGSAVDEEKLRFDFAWNKALSTAELAEVETIVRERIKEELPVHSYIAPLEKALNIKALRQVFGERYPDPVRVLSVGQE-VPPMLEDPANPAWEDKSVEFCGGTHLSNTRDAERFALIEETGIAKGIRRITAVTKDGAKAAEALAEEFAGLLAVAEGKPEDALEAEVKMLSMRLGPLSISAVEKERFRTGLEALVVRVKKYKKEKMAKNREKALGRVVEVATAAAAEGKGVVIERVDFGTDGKISKECLDKAAQVHPTGCFLLVSADEDTGKFGVYSSVPVKEASFDPVAWVAAAIEGAGGGRSGGKAPRGQGTGVGLENVEAALMAAK 990
            E  WP  +VR  F ++F Q  GHT+W SSP VP DDPTLLFTNAGMNQ+KPLFLGT DP + ++ L RAVNSQKCIRAGGKHNDL+DVGKD YHHTFFEMLGNWSFG YFKE+AI  AW+CL + + LDP RLYATYFGGD++     D EA+ +WLK+LP NRVLPF  KDNFWEMG TGPCGPC+EIHYDR+ GR+  +LVN D PD+IEIWN VFIQYNRE  G LR LP +HVDTGMGFERLTSI+QN  SNYDTDIFLPLF AIQ  TGA PY GK+GKED E  DMAYRVVADHIRTL FAI+DGA PSNDGRGYVLRRVLRRAVRYG++ L A  GFFSKLV  ++  M +VFP+L+EKE RV +V+ EEE +F+RT+D+G + F+++AE+               FP ++AH+LY+TMGFP DLTELMAEE+GL VD AGFE  + +++ LSA A  AK     GK++ + AEQTA L S+ V  TD S KYVW+ H  + KV AL+ G+  TED +GFVD +T E   + VILN TSFYAEQGGQI DTG L    G   +RV +VQ YG +VLHIG +E G + V DT TC VDYVRR  IA NHTMTHVLNHALR VL   V+D S   +QKGS VDE KLRFDF+WN  L+  +L +VE      I  ++PV  Y+APL  A  I +LR VFGE+YPDPVRV++V  + V  ML +P +  W + SVEFCGGTHL+NT +AE F L+ E GIAKG+RRI  VT + AK   ALA+ FA  L+ AE    DALE  VK+LS  L  L+ISA+ K  FR  L+ L   V  YKK+K+A   ++ + +   VA A    G  VV+ RVDF  DGK +K       +       +L++ D D  KF V +  P  +   D   WVAA  EG  GG+ GGKA   Q     L+ V+     AK
Sbjct:    8 EFKWPMAKVRTTFFEFF-QANGHTFWPSSPCVPVDDPTLLFTNAGMNQYKPLFLGTCDPNLEMASLKRAVNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKEEAIDLAWKCLTEVYQLDPNRLYATYFGGDEQTPC--DTEARDIWLKYLPENRVLPFDAKDNFWEMGATGPCGPCTEIHYDRIGGRDAAKLVNADLPDVIEIWNNVFIQYNREADGSLRPLPAQHVDTGMGFERLTSILQNVDSNYDTDIFLPLFAAIQRETGARPYQGKIGKEDPEFIDMAYRVVADHIRTLCFAIADGATPSNDGRGYVLRRVLRRAVRYGRQNLNAELGFFSKLVPTLIELMGDVFPELKEKELRVTQVIREEEESFSRTIDKGLQKFQELAEKCTDGV----------FPGEDAHYLYTTMGFPFDLTELMAEERGLKVDVAGFEARMKQEQELSAAAHLAKMTGDSGKDMRMVAEQTAHLVSKNVPSTDDSPKYVWNQHLENCKVEALFLGRNETEDKIGFVDVITPESSTIGVILNQTSFYAEQGGQINDTGFLKTTSGA-VVRVQNVQLYGQFVLHIGVVEQGTVSVNDTVTCEVDYVRRQPIASNHTMTHVLNHALRAVL---VNDESMTVEQKGSLVDETKLRFDFSWNGGLTPEQLKKVEEHCVSFIDRKVPVDCYVAPLPDAEKISSLRAVFGEKYPDPVRVVAVADKPVTDMLANPQDAVWNEYSVEFCGGTHLTNTSEAEAFVLLSEEGIAKGVRRIVGVTMNDAKKCMALADSFADKLSSAEKLEGDALEPAVKVLSTELDGLNISAIRKIEFREKLQELSKAVVAYKKQKVAGMADEIVNQA--VALAGTMAGSKVVL-RVDFAIDGKTAKSVAQAFGKKVTDKALMLIAVDVD--KFSVVAFSP--KGVVDCQKWVAAVTEGT-GGKGGGKADFAQTQVSDLKFVDQVEQKAK 952          

BLAST of NO08G02150 vs. NCBI_GenBank
Match: GAX12815.1 (alanyl-tRNA synthetase [Fistulifera solaris])

HSP 1 Score: 1025.8 bits (2651), Expect = 9.100e-296
Identity = 552/970 (56.91%), Postives = 667/970 (68.76%), Query Frame = 0

Query:   23 ETAWPARRVRQIFIDYFVQKRGHTYWSSSPVVPHDDPTLLFTNAGMNQFKPLFLGTADPKMPLSKLNRAVNSQKCIRAGGKHNDLEDVGKDTYHHTFFEMLGNWSFGSYFKEDAITWAWECLIDEFGLDPTRLYATYFGGDDKQGLKPDEEAKQLWLKFLPANRVLPFGCKDNFWEMGETGPCGPCSEIHYDRLSGREVPELVNMDSPDLIEIWNIVFIQYNREEGGELRELPNKHVDTGMGFERLTSIMQNRPSNYDTDIFLPLFDAIQELTGAAPYAGKLGKEDKESKDMAYRVVADHIRTLTFAISDGAVPSNDGRGYVLRRVLRRAVRYGQKVLGAPPGFFSKLVHVVVNQMKEVFPDLEEKEKRVMEVLAEEERAFNRTLDQGEKHFKKVAEELLLSASGSGNVGKVKFPAKEAHHLYSTMGFPIDLTELMAEEKGLTVDRAGFETLLAEDKRLSAMAAAAKKA-LGGKELVLEAEQTAWLQSRGVEMTDSSLKYVWHHE-PSAKVLALYKGKGSTEDGVGFVDEVTAEDGIVAVILNTTSFYAEQGGQIYDTGVLTNLDGTDTLRVDSVQSYGGYVLHIGPLESGALRVGDTATCMVDYVRRANIAPNHTMTHVLNHALRRVLMKGVSDGSGLCDQKGSAVDEEKLRFDFAWNKALSTAELAEVETIVRERIKEELPVHSYIAPLEKALNIKALRQVFGERYPDPVRVLSVG-QEVPPMLEDPANPAWEDKSVEFCGGTHLSNTRDAERFALIEETGIAKGIRRITAVTKDGAKAAEALAEEFAGLLAVAEGKPEDALEAEVKMLSMRLGPLSISAVEKERFRTGLEALVVRVKKYKKEKMAKNREKALGRVVEVATAAAAEGKGVVIERVDFGTDGKISKECLDKAAQVHPTGCFLLVSADEDTGKFGVYSSVPVKEASFDPVAWVAAAIEGAGGGRSGGKAPRGQGTGVGLENVEAALMAAK 990
            E  WP  +VR  F ++F Q  GHT+W SSP VP DDPTLLFTNAGMNQ+KPLFLGT DP + ++ L RAVNSQKCIRAGGKHNDL+DVGKD YHHTFFEMLGNWSFG YFKE+AI  AW+CL + + LDP RLYATYFGGD++     D EA+ +WLK+LP NRVLPF  KDNFWEMG TGPCGPC+EIHYDR+ GR+  +LVN D PD+IEIWN VFIQYNRE  G LR LP +HVDTGMGFERLTSI+QN  SNYDTDIFLPLF AIQ  TGA PY GK+GKED E  DMAYRVVADHIRTL FAI+DGA PSNDGRGYVLRRVLRRAVRYG++ L A  GFFSKLV  ++  M +VFP+L+EKE RV +V+ EEE +F+RT+D+G + F+++AE+               FP ++AH+LY+TMGFP DLTELMAEE+GL VD AGFE  + +++ LSA A  AK A   GK++ + AEQTA L S+ V  TD S KYVW+    + KV AL+ G+  TED +GFVD +T E   + VILN TSFYAEQGGQI DTG L    G   ++V +VQ YG +VLHIG +E G + V D+ TC VDYVRR  IA NHTMTHVLNHALR VL   V+D S   +QKGS VDE KLRFDF+WN  L+  +L +VE      I  ++PV  Y+APL  A  I +LR VFGE+YPDPVRV++V  Q V  ML +P +  W + SVEFCGGTHL+NT +AE F L+ E GIAKG+RRI  VT D AK   ALA+ FA  L  AE    D LE  VK+LS  L  L++SA+ K  FR  L+ L   V  YKK+K+A   ++ + +   VA A    G  VV+ RVD   DGK +K       +       +L++ D D  KF V +  P  +   D   WVAA  EG  GG+ GGKA   Q     L+ V+     AK
Sbjct:    8 ELKWPMAKVRTTFFEFF-QANGHTFWPSSPCVPVDDPTLLFTNAGMNQYKPLFLGTCDPNLEMASLKRAVNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKEEAIDLAWKCLTEVYQLDPNRLYATYFGGDEQTPC--DTEARDIWLKYLPENRVLPFDAKDNFWEMGATGPCGPCTEIHYDRIGGRDAAKLVNADLPDVIEIWNNVFIQYNREADGSLRPLPAQHVDTGMGFERLTSILQNVDSNYDTDIFLPLFAAIQRETGARPYQGKIGKEDPEFIDMAYRVVADHIRTLCFAIADGATPSNDGRGYVLRRVLRRAVRYGRQNLNAELGFFSKLVPTLIELMGDVFPELKEKELRVTQVIREEEESFSRTIDKGLQKFQELAEKCTDGV----------FPGEDAHYLYTTMGFPFDLTELMAEERGLKVDVAGFEARMKQEQELSAAAHLAKMAGDSGKDMRMVAEQTAHLVSKNVPSTDDSPKYVWNQPLENCKVEALFLGRNETEDKIGFVDVITPESSTIGVILNQTSFYAEQGGQINDTGFLKTTSGA-VVKVQNVQLYGKFVLHIGVVEQGTVSVNDSVTCEVDYVRRQLIASNHTMTHVLNHALRAVL---VNDESMTVEQKGSLVDETKLRFDFSWNGGLTPEQLKKVEEHCVSFIDRKVPVDCYVAPLPDAEKISSLRAVFGEKYPDPVRVVAVADQPVTDMLANPQDAVWNEYSVEFCGGTHLTNTSEAEAFVLLSEEGIAKGVRRIVGVTMDDAKKCMALADSFADKLRNAETLQGDVLEPAVKVLSTELDGLNVSAIRKIEFREKLQELSKAVVAYKKQKVAGMADEIVNQA--VALAGTTAGSKVVL-RVDLAMDGKTAKSVAQAFGKKVTDKALMLIAVDVD--KFSVVAFSP--KGVVDCQKWVAAVTEGT-GGKGGGKADFAQTQVGDLKFVDQVEQKAK 952          

BLAST of NO08G02150 vs. NCBI_GenBank
Match: XP_002290312.1 (alanine-trna ligase [Thalassiosira pseudonana CCMP1335] >EED92064.1 alanine-trna ligase [Thalassiosira pseudonana CCMP1335])

HSP 1 Score: 1020.8 bits (2638), Expect = 2.900e-294
Identity = 530/972 (54.53%), Postives = 674/972 (69.34%), Query Frame = 0

Query:   26 WPARRVRQIFIDYFVQKRGHTYWSSSPVVPHDDPTLLFTNAGMNQFKPLFLGTADPKMPLSKLNRAVNSQKCIRAGGKHNDLEDVGKDTYHHTFFEMLGNWSFGSYFKEDAITWAWECLIDEFGLDPTRLYATYFGGDDKQGLKPDEEAKQLWLKFLPANRVLPFGCKDNFWEMGETGPCGPCSEIHYDRLSGREVPELVNMDSPDLIEIWNIVFIQYNREEGGELRELPNKHVDTGMGFERLTSIMQNRPSNYDTDIFLPLFDAIQELTGAAPYAGKLGKEDKESKDMAYRVVADHIRTLTFAISDGAVPSNDGRGYVLRRVLRRAVRYGQKVLGAPPGFFSKLVHVVVNQMKEVFPDLEEKEKRVMEVLAEEERAFNRTLDQGEKHFKKVAEELLLSASGSGNVGKVK-FPAKEAHHLYSTMGFPIDLTELMAEEKGLTVDRAGFETLLAEDKRLSAMAAAAKKALG-GKELVLEAEQTAWLQSRGVEMTDSSLKYVWHHEPSAKVL-ALYKGKGSTEDGVGFVDEVTAEDGIVAVILNTTSFYAEQGGQIYDTGVLTNLDGTDTLRVDSVQSYGGYVLHIGPLESGALRVGDTATCMVDYVRRANIAPNHTMTHVLNHALRRVLM-KGVSDGSGL-CDQKGSAVDEEKLRFDFAWNKALSTAELAEVETIVRERIKEELPVHSYIAPLEKALNIKALRQVFGERYPDPVRVLSVG-QEVPPMLEDPANPAWEDKSVEFCGGTHLSNTRDAERFALIEETGIAKGIRRITAVTKDGAKAAEALAEEFAGLLAVAEGKPEDALEAEVKMLSMRLGPLSISAVEKERFRTGLEALVVRVKKYKKEKMAKNREKALGRVVEVATAAAAEGKGVVIERVDFGTDGKISKECLDKAAQVHPTGCFLLVSADEDTGKFGVYSSVPVKEASFDPVAWVAAAIEGAGGGRSGGKAPRGQGTGVGLENVEAALMAAKRF 992
            WP  +VR  FI YF ++ GHT+W SSP VPHDDPTLLFTNAGMNQ+KPLFLGT DP + ++ L RA NSQKCIRAGGKHNDL+DVGKD YHHTFFEMLGNWSFG YFK++AI  AW+CL +EF LDP RLYATYFGGD+      D+EA+++WL++LPA RVLPF  KDNFWEMG TGPCGPC+EIHYDR+  R+  +LVN D PD+IEIWN VFIQ+NRE  G LR LP +HVDTGMGFERLTSI+Q   SNYDTDIF+PLFDAIQ++TGA PYAGK+ KED E  DMAYRVVADH+RTL+FAI+DGAVPSNDGRGYVLRRVLRRAVRYG++ L A  GFF++LV  +V  M + FP+L EKE+ V  ++ EEE +F++T+D+G   F K  + L          G VK FP ++AH LY++MGFP+DLTELM EE+GLT+D  G++  + E++ LS  A  AK + G GK++ L AEQT+ L ++GV+ T+   KYVW+ +    ++ AL+ G+  TEDG+GFVD ++ E+G V ++L+ T+FY E GGQ +DTG +    G  T+ + +VQ YG +VLHIG +  G + V D ATC VDYVRRA IA NHTMTH+LN ALR VL+ +G +D  G   DQKGS VDE KLRFDF+W+  L+  +L  VE I  ++I   +PV +Y+APLE A  I +LR VFGE YPDPVRV++V    +  MLE+P +  W   SVEFCGGTHL NT +A+ F L+ E GIAKG+RRIT VT   A  A A A EF   L  A       LEA VK+L++ +  LSISA +K +FR  +     +V  +KK   A+     + +V+E A   + EG  VV+ R DFG DGK++K       +       L V+AD D  +F V +  P K +  D  AWVAAA+ G   G+ GGK    Q T  G+ N++  L  A+ F
Sbjct:   14 WPMDKVRSTFISYF-ERNGHTFWRSSPCVPHDDPTLLFTNAGMNQYKPLFLGTCDPALKMASLKRATNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEAIEMAWKCLTEEFKLDPNRLYATYFGGDESTPC--DDEAREIWLQYLPAERVLPFDAKDNFWEMGATGPCGPCTEIHYDRIGNRDASKLVNADLPDVIEIWNNVFIQFNREADGSLRPLPAQHVDTGMGFERLTSILQGVDSNYDTDIFIPLFDAIQKITGARPYAGKIEKEDPEFIDMAYRVVADHVRTLSFAIADGAVPSNDGRGYVLRRVLRRAVRYGRQNLNAELGFFAQLVRTLVELMGDTFPELREKEEYVTAIIREEEESFSKTVDKGIVEFNKRCDAL----------GDVKVFPGEDAHFLYTSMGFPVDLTELMCEERGLTLDMEGYDAKMKEEQELSNAAHLAKMSAGSGKDMRLVAEQTSHLVNKGVDATNDDAKYVWNEDLEGSIIKALFIGRNETEDGIGFVDSISKENGAVGIVLDKTNFYGESGGQTFDTGSIKTSSGA-TIEITNVQIYGSFVLHIGDVTEGTVTVNDAATCCVDYVRRAPIAANHTMTHILNFALRDVLVTRGDADQHGASVDQKGSLVDENKLRFDFSWSGPLTPEQLVAVEKICVDQINSAVPVDNYVAPLEGAQQISSLRAVFGEVYPDPVRVVAVSPSPITSMLENPQDAQWNKYSVEFCGGTHLKNTSEAQGFVLLSEEGIAKGVRRITGVTMKDAAIATAAASEFEAKLTAAGSLEGLDLEAHVKVLTVEMEGLSISAAKKIQFRDVMGTYGKKVAAWKKANAAEQTATVVEQVIEAAN--STEGNKVVV-RCDFGVDGKVAKAVSTAYGKKIKDKALLFVTADVDADRFMVVAFAP-KGSDVDCKAWVAAAVAGT-DGKGGGKKDSAQSTVPGVGNIDGVLEKARGF 966          

BLAST of NO08G02150 vs. NCBI_GenBank
Match: EJK71470.1 (hypothetical protein THAOC_07085 [Thalassiosira oceanica])

HSP 1 Score: 1013.1 bits (2618), Expect = 6.100e-292
Identity = 532/974 (54.62%), Postives = 669/974 (68.69%), Query Frame = 0

Query:   24 TAWPARRVRQIFIDYFVQKRGHTYWSSSPVVPHDDPTLLFTNAGMNQFKPLFLGTADPKMPLSKLNRAVNSQKCIRAGGKHNDLEDVGKDTYHHTFFEMLGNWSFGSYFKEDAITWAWECLIDEFGLDPTRLYATYFGGDDKQGLKPDEEAKQLWLKFLPANRVLPFGCKDNFWEMGETGPCGPCSEIHYDRLSGREVPELVNMDSPDLIEIWNIVFIQYNREEGGELRELPNKHVDTGMGFERLTSIMQNRPSNYDTDIFLPLFDAIQELTGAAPYAGKLGKEDKESKDMAYRVVADHIRTLTFAISDGAVPSNDGRGYVLRRVLRRAVRYGQKVLGAPPGFFSKLVHVVVNQMKEVFPDLEEKEKRVMEVLAEEERAFNRTLDQGEKHFKKVAEELLLSASGSGNVGKVK-FPAKEAHHLYSTMGFPIDLTELMAEEKGLTVDRAGFETLLAEDKRLSAMAAAAKKALG-GKELVLEAEQTAWLQSRGVEMTDSSLKYVWHHE-PSAKVLALYKGKGSTEDGVGFVDEVTAEDGIVAVILNTTSFYAEQGGQIYDTGVLTNLDGTDTLRVDSVQSYGGYVLHIGPLESGALRVGDTATCMVDYVRRANIAPNHTMTHVLNHALRRVLMKGV--SDGSGLCDQKGSAVDEEKLRFDFAWNKALSTAELAEVETIVRERIKEELPVHSYIAPLEKALNIKALRQVFGERYPDPVRVLSVGQE-VPPMLEDPANPAWEDKSVEFCGGTHLSNTRDAERFALIEETGIAKGIRRITAVTKDGAKAAEALAEEFAGLLAVAEGKPEDALEAEVKMLSMRLGPLSISAVEKERFRTGLEALVVRVKKYKKEKMAKNREKALGRVVEVATAAAAEGKGVVIERVDFGTDGKISKECLDKAAQVHPTGCFLLVSADEDTGKFGVYSSVPVKEASFDPVAWVAAAIEGAGGGRSGGKAPRGQGTGVGLENVEAALMAAKRF 992
            T WP  +VR  FI +F ++ GHT+W SSP VP+DDPTLLFTNAGMNQ+KPLFLGT DP + ++ L RA NSQKCIRAGGKHNDL+DVGKD YHHTFFEMLGNWSFG YFK++AI  AW+CL +EFGLDP RLYATYFGGD+      D+EA+ +WL+FLPA RVLPF  KDNFWEMG TGPCGPC+EIHYDR+  R+   LVN D PD+IEIWN VFIQ+NRE  G LR LP +HVDTGMGFERLTSI+Q   SNYDTDIF+PLFDAI+ +TGA PYAGK+G ED+   DMAYRVVADH+RTL+FAI+DGAVPSNDGRGYVLRRVLRRAVRYG++ L A  GFF+KLV  +V+ M + FP+L EKE+ +  V+ EEE +F++T+D+G   FKK+ + L          G  K FP  +A  L++  GFP+DLTELM EE+GL +D AGF+  L E++  S  A   K + G GK++ L AEQT+ L  +GV+ TD S KYVW  +   + V AL+ G+  TEDGVGFVD +T E G V ++L+ T+FY E GGQIYDTG +    G  T+ V +VQ YG +VLH+G ++SG + V ++ATC VDY RR+ +A NHTMTHVLNHALR VL+     S      DQKGS  DE KLRFDF+W+ AL+  +LA VE +  E I+  +PV + +A L+ A +I +LR VFGE YPDPVRV++V    +  ML +P++  W D SVEFCGGTHL NT +A+ F L+ E GIAKGIRRITAVT   A  A A A EF G +  A      ALEA+VK+L++ LG LSISA  K   R  L +   RV  +KK+  A+   + + +VVE   A ++ G  VVI R DFG DGK++K       +       +LVSADE + ++ V +  P      D  AW AAA EG  G + GGK      +  G+  +   L  A+ F
Sbjct:  117 TEWPMDKVRSTFISFF-ERNGHTFWRSSPCVPYDDPTLLFTNAGMNQYKPLFLGTVDPNVEMAGLKRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEAIEMAWKCLTEEFGLDPERLYATYFGGDESTPC--DDEARDIWLQFLPAERVLPFDAKDNFWEMGATGPCGPCTEIHYDRIGNRDAASLVNADLPDVIEIWNNVFIQFNREADGSLRPLPAQHVDTGMGFERLTSILQGVDSNYDTDIFIPLFDAIRSITGARPYAGKIGSEDEGYVDMAYRVVADHVRTLSFAIADGAVPSNDGRGYVLRRVLRRAVRYGRQNLNAELGFFAKLVRTLVDLMGDTFPELREKEEYITSVIREEEESFSKTVDKGIVEFKKMCDAL----------GDQKTFPGADAFTLFAERGFPVDLTELMCEERGLVLDTAGFDAKLKEEQDKSKEAHLKKMSAGTGKDMRLVAEQTSHLVGKGVDPTDDSAKYVWDDDLTGSTVKALFVGRNETEDGVGFVDSITPESGAVGIVLDKTNFYGEAGGQIYDTGSIVAASGA-TIEVTNVQVYGSFVLHLGEVKSGTVTVDESATCKVDYARRSPVAANHTMTHVLNHALRDVLVTRAESSSAGAAVDQKGSLCDESKLRFDFSWSGALTADQLAAVERMCVETIERGVPVDTLVAALDDARSISSLRAVFGETYPDPVRVVAVSSSPISDMLANPSDERWNDYSVEFCGGTHLKNTSEAKGFVLLSEEGIAKGIRRITAVTMKDAADATARAAEFEGRVVEAGKLDGFALEAQVKLLTVELGGLSISAAMKMTLRDTLGSYGKRVVAWKKKNAAEQTSRVVDQVVE--AAGSSSGDKVVI-RCDFGCDGKVAKAVTAAYGKKVKDKALMLVSADESSDRYMVVAFAPKGMKGVDCKAWAAAATEGT-GAKGGGKKDSANFSVQGVSTIGGVLEKARSF 1072          

BLAST of NO08G02150 vs. NCBI_GenBank
Match: XP_009516106.1 (hypothetical protein PHYSODRAFT_473521 [Phytophthora sojae] >EGZ28831.1 hypothetical protein PHYSODRAFT_473521 [Phytophthora sojae])

HSP 1 Score: 999.2 bits (2582), Expect = 9.100e-288
Identity = 520/975 (53.33%), Postives = 659/975 (67.59%), Query Frame = 0

Query:   26 WPARRVRQIFIDYFVQKR--GHTYWSSSPVVPHDDPTLLFTNAGMNQFKPLFLGTADPKMPLSKLNRAVNSQKCIRAGGKHNDLEDVGKDTYHHTFFEMLGNWSFGSYFKEDAITWAWECLIDEFGLDPTRLYATYFGGDDKQGLKPDEEAKQLWLKFLPANRVLPFGCKDNFWEMGETGPCGPCSEIHYDRLSGREVPELVNMDSPDLIEIWNIVFIQYNREEGGELRELPNKHVDTGMGFERLTSIMQNRPSNYDTDIFLPLFDAIQELTGAAPYAGKLGKEDKESKDMAYRVVADHIRTLTFAISDGAVPSNDGRGYVLRRVLRRAVRYGQKVLGAPPGFFSKLVHVVVNQMKEVFPDLEEKEKRVMEVLAEEERAFNRTLDQGEKHFKKVAEELLLSASGSGNVGKVKFPAKEAHHLYSTMGFPIDLTELMAEEKGLTVDRAGFETLLAEDKRLSAMAAAAKKALGGKELVLEAEQTAWLQSRGVEMTDSSLKYVWHHEPSAKVLALYKGKGSTEDGVGFVDEVTAED-GIVAVILNTTSFYAEQGGQIYDTGVLTNLDGTDTLRVDSVQSYGGYVLHIGPLESGALRVGDTATCMVDYVRRANIAPNHTMTHVLNHALRRVLMKGVSDGSGLCDQKGSAVDEEKLRFDFAWNKALSTAELAEVETIVRERIKEELPVHSYIAPLEKALNIKALRQVFGERYPDPVRVLSVGQEVPPMLEDPANPAWEDKSVEFCGGTHLSNTRDAERFALIEETGIAKGIRRITAVTKDGAKAAEALAEEFAGLLAVAEGKPEDALEAEVKMLSMRLGPLSISAVEKERFRTGLEALVVRVKKYKKEKMAKNREKALGRVVEVATAAAAEGKGVVIERVDFGTDGKISKECLDKAAQVHPTGCFLLVSADEDTGKFGVYSSVP---VKEASFDPVAWV---AAAIEGAGGGRSGGKAPRGQGTGVGLENVEAALMAAKRF 992
            WPA R+R+ F+D+F  +    HT+W S PVVP DDPTLLF NAGMNQ+KP+FLG  DP  P+SKL RA NSQKCIRAGGKHNDL+DVGKD YHHTFFEMLGNWSFG+YFK++A+  ++  L + FG+D  RLYATYFGGD+KQGL  DEE +Q+WL +LPA RVLPFGCKDNFWEMG+ GPCGPC+EIHYDR+  R+  +LVN D PD+IEIWN VFIQ+NRE+ G+L  LP+KHVDTGMGFERL SI+Q + SNYDTD+F PLF AIQ+ T AAPY GKLGKED + KDMAYRVVADH+RTLTFAI+DGAVPSNDGRGYVLRR+LRRAVRYGQ+ L AP GF ++LV VVVN + E FP+L +K+K V E++ +EE++F RTL++G + FKK+A+ +  + +GS     +  P ++A  LY +MGFPIDLTE+M EE+G+TVD  G+E  +      S          G + LVLEA +T+ L  + +  TD   KY W  + SAKV+A++    +TE    FVDEV A D   V VIL+ TSFYA+ GGQIYDTGV++       L VDSV+SY GYV+H+GP+ SG+++VGD   C VDY RR+ IAPNHTMTHVLN ALR+VL   V       DQ+GS VDE +LRFDF  NKAL   +LAEVE I  + IK++L V++  +   +A  I+ LR VFGE YPD VRV+S+GQ + PMLEDP N  W + SVEFCGGTHL NT++A++F L EE  IAKGIRR++A T D AK AE    +    L   +    +     V      L    IS   K++ R  ++ LV RVKK KKE  A      +      AT A   G+ +V+ + D GTD K+ +E L+    + PTG F++ S D D  K   ++ V    V     D   WV    A ++G GGG+    A    G    +E V+ A+  AK F
Sbjct:   22 WPAARIRKTFVDFFTNQTELPHTFWKSCPVVPLDDPTLLFINAGMNQYKPIFLGQVDPSHPMSKLVRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGNYFKKEAVHMSFTLLTEVFGIDKERLYATYFGGDEKQGLPSDEETRQIWLNYLPAERVLPFGCKDNFWEMGDVGPCGPCTEIHYDRIGNRDASKLVNADLPDVIEIWNNVFIQFNREQDGKLVPLPHKHVDTGMGFERLASILQGKDSNYDTDVFTPLFAAIQKSTKAAPYTGKLGKEDPDKKDMAYRVVADHVRTLTFAITDGAVPSNDGRGYVLRRILRRAVRYGQQFLNAPCGFLTELVPVVVNMLGEAFPELIQKQKEVEEIILDEEKSFGRTLNKGIERFKKIAQGIREANAGSDKA--LVVPGEDAFFLYDSMGFPIDLTEIMVEEEGMTVDVKGYEECMRLQSERSKTDRKKGGNNGARPLVLEARETSALAGKSITATDDMAKYEWSIKTSAKVVAIFT---TTESSSDFVDEVKAGDFERVGVILDKTSFYAQAGGQIYDTGVVSG--ANFKLDVDSVESYAGYVMHMGPIASGSIKVGDAVECQVDYARRSKIAPNHTMTHVLNFALRKVLGTTV-------DQRGSLVDESRLRFDFTNNKALKANQLAEVEAICDDIIKQQLEVYTQDSAQAEAKRIQGLRAVFGETYPDFVRVVSIGQPIAPMLEDPENSTWSNFSVEFCGGTHLKNTKEAKKFVLYEEGAIAKGIRRVSAYTCDLAKEAEERGAKLQAELNAIDMMDGNEFVERVSAFKPVLDQALISLPLKDQLRKQVDGLVNRVKKIKKEAAAARAANGVRDATAEATKAKEAGQEIVVVKFDVGTDSKLGREMLEAMTTIIPTGSFMIFSTDSDANKTAAFTQVSQHHVDSKQLDARKWVNHAMAVMKGKGGGKD---ALNATGQAKTVEKVDEAVALAKAF 979          

BLAST of NO08G02150 vs. NCBI_GenBank
Match: OEU13746.1 (alanyl-tRNA synthetase, cytoplasmic [Fragilariopsis cylindrus CCMP1102])

HSP 1 Score: 996.9 bits (2576), Expect = 4.500e-287
Identity = 541/992 (54.54%), Postives = 670/992 (67.54%), Query Frame = 0

Query:   16 SVTDLRVETAWPARRVRQIFIDYFVQKRGHTYWSSSPVVPHDDPTLLFTNAGMNQFKPLFLGTADPKMPLSKLNRAVNSQKCIRAGGKHNDLEDVGKDTYHHTFFEMLGNWSFGSYFKEDAITWAWECLIDEFGLDPTRLYATYFGGDDKQGLKPDEEAKQLWLKFLPANRVLPFGCKDNFWEMGETGPCGPCSEIHYDRLSGREVPELVNMDSPDLIEIWNIVFIQYNREEGGELRELPNKHVDTGMGFERLTSIMQNRPSNYDTDIFLPLFDAIQELTGA-APYAGKLGK-EDKESKDMAYRVVADHIRTLTFAISDGAVPSNDGRGYVLRRVLRRAVRYGQKVLGAPPGFFSKLVHVVVNQMKEVFPDLEEKEKRVMEVLAEEERAFNRTLDQGEKHFKKVAEELLLSASGSGNVGKVKFPAKEAHHLYSTMGFPIDLTELMAEEKGLTVDRAGFETLLAEDKRLSAMAAAAKKALG-GKELVLEAEQTAWL-QSRGVEMTDSSLKYVWHHEPSAKVLALYKGKGSTEDGVGFVDEVTAEDGIVAVILNTTSFYAEQGGQIYDTGVLTNLDGTDTLRVDSVQSYGGYVLHIGPLESGALRVGDTATCMVDYVRRANIAPNHTMTHVLNHALRRVLMK------GVSDGSGLCDQKGSAVDEEKLRFDFAWNKALSTAELAEVETIVRERIKEELPVHSYIAPLEKALNIKALRQVFGERYPDPVRVLSV-GQEVPPMLEDPANPAWEDKSVEFCGGTHLSNTRDAERFALIEETGIAKGIRRITAVTKDGAKAAEALAEEF-AGLLAVAEGKPEDALEAEVKMLSMRLGPLSISAVEKERFRTGLEALVVRVKKYKKEKMAKNREKALGRVVEVATAAAAEGK----GVVIERVDFGTDGKISKECLDKAAQVHPTGCFLLVSADEDTGKFGVYSSVPVKEASFDPVAWVAAAIEGAGGGRSGGKAPRGQGTGVGLENVEAALMAAKRF 992
            SV D + E  WP  +VR  FI++F +K GHTYW SSP VP DDPTLLFTNAGMNQ+KPLFLGT DP +P+SKL RA NSQKCIRAGGKHNDL+DVGKD YHHTFFEMLGNWSFG YFKE+ I  +W CL+DEF LDP RLYA+YFGGD+     PD EA+ +WLK+LPA RVLPF   DNFWEMG TGPCGPC EIHYDR+ GR+   LVN D PD+IEIWN VFIQ+NRE  G LR LP++HVDTGMGFERLTSI+Q   SNYDTDIF PLF+ I+ LT A  PY GK+G  ED    DMAYRV+ADH+RTL+FAI DGAVPSNDGRGYVLRRVLRRAVRYG++ LGA  GFFS LV  +V  M   FP+L E++  ++ V+ EEE +F+RTLD+G + F  +       AS   N G   F  K+AH+LY+TMGFPIDLTELMAEE G+TVD+ GFE  + E++ +S  A A K A G GK++ + AEQT+ L  +  V  TD S KY       + V AL+ G+G TED +GFVD VT E   V +IL+ TSFYAEQGGQ YDTG +  +  + T R+ +VQ YG +VLH+G +  G L VGD+ +C VDYVRR  I+ NHTMTHVLN+ALR +L+        VS G  + DQKGS VDE K RFDF+WN  L+  +LA+VET+VR++I +E PV  ++APL  A  I ALR VFGE+YPDPVR+++V    V  +L +P +  W   SVEFCGGTHL+NT++AE F L+ E GIAKGIRRI  VT D AK A A A+EF   L A ++    D LEA VK+LSM    L +S V K  FR  +  L+ +V  YKK++MA       G   E+   A A G+      VI R DFG + K+ K       +       +++SAD D  +F V +  P K    D  AW  A IEG   G+ GGK    Q T   +  +++A+  A+++
Sbjct:    2 SVEDDKKELEWPMDKVRSTFINFFKEKHGHTYWGSSPCVPVDDPTLLFTNAGMNQYKPLFLGTCDPALPMSKLTRATNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKEETIAMSWSCLVDEFKLDPNRLYASYFGGDET--TPPDLEARDIWLKYLPAERVLPFDAADNFWEMGATGPCGPCVEIHYDRIGGRDAAHLVNADVPDVIEIWNNVFIQFNREADGSLRPLPSQHVDTGMGFERLTSILQGVDSNYDTDIFQPLFEEIRILTNAPRPYGGKVGPVEDIGFIDMAYRVIADHVRTLSFAIVDGAVPSNDGRGYVLRRVLRRAVRYGRQNLGAELGFFSNLVPKLVEIMGGTFPELIERQDYIISVVREEEESFSRTLDKGLQKFTDL-------ASNEDNNG--VFSGKDAHYLYATMGFPIDLTELMAEELGMTVDKDGFEAKMKEEQEMSQAAHAEKMAAGSGKDMRMVAEQTSHLVHTLSVPATDDSAKYTTEVLDGSIVKALFIGRGETEDQIGFVDSVTKESSTVGIILDRTSFYAEQGGQTYDTGDIVKVGDSATFRITNVQLYGSFVLHLGIVIDGELSVGDSVSCSVDYVRRRPISSNHTMTHVLNYALREILVNRPAAEGKVSTGVNV-DQKGSLVDEFKSRFDFSWNGQLTPQQLADVETLVRDQIVKETPVDCFVAPLADAEKIGALRAVFGEKYPDPVRIVAVTPSPVTEILANPQDAMWNGYSVEFCGGTHLTNTKEAEAFCLLNEEGIAKGIRRIIFVTLDDAKKAIATADEFETRLEAASKLDGGDELEALVKVLSMECNSLVVSTVRKTSFRETIADLLKKVMAYKKQRMA-------GMTDEIKAKAIAAGEVTDGNKVIFRFDFGANAKLGKNVATAFTKKITDKAIMIISADSDADRFLVLAMAP-KGVDVDCKAWCNAVIEGT-DGKGGGKKDSAQFTVPDISLLDSAIAKAEQW 972          

BLAST of NO08G02150 vs. NCBI_GenBank
Match: ETI30952.1 (alanine-tRNA ligase [Phytophthora parasitica P1569] >ETM31265.1 alanine-tRNA ligase [Phytophthora parasitica])

HSP 1 Score: 995.0 bits (2571), Expect = 1.700e-286
Identity = 524/994 (52.72%), Postives = 668/994 (67.20%), Query Frame = 0

Query:   10 VDDLRESVTDL---RVETAWPARRVRQIFIDYFVQKRG--HTYWSSSPVVPHDDPTLLFTNAGMNQFKPLFLGTADPKMPLSKLNRAVNSQKCIRAGGKHNDLEDVGKDTYHHTFFEMLGNWSFGSYFKEDAITWAWECLIDEFGLDPTRLYATYFGGDDKQGLKPDEEAKQLWLKFLPANRVLPFGCKDNFWEMGETGPCGPCSEIHYDRLSGREVPELVNMDSPDLIEIWNIVFIQYNREEGGELRELPNKHVDTGMGFERLTSIMQNRPSNYDTDIFLPLFDAIQELTGAAPYAGKLGKEDKESKDMAYRVVADHIRTLTFAISDGAVPSNDGRGYVLRRVLRRAVRYGQKVLGAPPGFFSKLVHVVVNQMKEVFPDLEEKEKRVMEVLAEEERAFNRTLDQGEKHFKKVAEELLLSASGSGNVGKVKFPAKEAHHLYSTMGFPIDLTELMAEEKGLTVDRAGFETLLAEDKRLSAMAAAAKKALGGKELVLEAEQTAWLQSRGVEMTDSSLKYVWHHEPSAKVLALYKGKGSTEDGVGFVDEVTAED-GIVAVILNTTSFYAEQGGQIYDTGVLTNLDGTDTLRVDSVQSYGGYVLHIGPLESGALRVGDTATCMVDYVRRANIAPNHTMTHVLNHALRRVLMKGVSDGSGLCDQKGSAVDEEKLRFDFAWNKALSTAELAEVETIVRERIKEELPVHSYIAPLEKALNIKALRQVFGERYPDPVRVLSVGQEVPPMLEDPANPAWEDKSVEFCGGTHLSNTRDAERFALIEETGIAKGIRRITAVTKDGAKAAEALAEEFAGLLAVAEGKPEDALEAEVKMLSMRLGPLSISAVEKERFRTGLEALVVRVKKYKKEKMAKNREKALGRVVEVATAAAAEGKGVVIERVDFGTDGKISKECLDKAAQVHPTGCFLLVSADEDTGKFGVYSSVP---VKEASFDPVAWV---AAAIEGAGGGRSGGKAPRGQGTGVGLENVEAALMAAKRF 992
            V+ L   V DL    VE  WPA R+R+ F++YF  ++   HT++ S PVVP DDPTLLF NAGMNQ+KP+FLG  DP  P++KL RA NSQKCIRAGGKHNDL+DVGKD YHHTFFEMLGNWSFG+YFK++A+  ++  L + FG+D  RLYATYFGGD+KQGL  DEE +++WL +LPA RVLPFGCKDNFWEMG+ GPCGPC+EIHYDR+  R+  +LVN D PD+IEIWN VFIQ+NRE+ G+L  LP+KHVDTGMGFERL SI+Q + SNYDTD+F PLF AIQ+ T AAPY GKLGKED + KDMAYRVVADH+RTLTFAI+DGAVPSNDGRGYVLRR+LRRAVRYGQ+ L AP GF ++LV VVVN + E FP+L +K+K V E++ +EE++F RTL++G + FKK+A+       G     K+  P ++A  LY +MGFPIDLTE+MAEE+G+TVD  G+E  +      S M      + G + LVLEA +T+ L  + +  TD   KY W+ +  AKV+A++    +TE    FVDEV A D   V VIL+ TSFY++ GGQIYDTGVL+       L VDSV+SY GYV+H+GP+ SG+++VGD+  C VDY RRA IAPNHTMTH LN ALR+VL         + DQ+GS VDE +LRFDF  NKAL   +LAEVE I  + IK++L V++  +   +A  I+ LR VFGE YPD VRV+S+GQ + PMLEDP N  W + SVEFCGGTHL NT++A++F L EE  IAKGIRR++A T D A  AE    +    L   +    +     V      L    IS   K++ R  ++ LV RVKK KKE  A      +      AT A   G+ +VI + D GTD K+ +E L+  + + PTG F++ S D D  K   ++ V    V     D   WV    A ++G GGG+    A    G    +E V+ A+  AK F
Sbjct:   22 VEALEGQVADLSPFSVEN-WPAARIRKTFVEYFENQKDLPHTFYKSCPVVPLDDPTLLFINAGMNQYKPIFLGQVDPSHPMAKLVRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGNYFKKEAVHLSFTLLTEVFGIDKDRLYATYFGGDEKQGLPSDEETREIWLNYLPAERVLPFGCKDNFWEMGDVGPCGPCTEIHYDRIGNRDASKLVNADLPDVIEIWNNVFIQFNREQDGKLVPLPHKHVDTGMGFERLASILQGKDSNYDTDVFTPLFAAIQKSTKAAPYTGKLGKEDPDKKDMAYRVVADHVRTLTFAITDGAVPSNDGRGYVLRRILRRAVRYGQQFLNAPSGFLTELVPVVVNMLGEAFPELIQKQKEVEEIILDEEKSFGRTLNKGIERFKKIAQ-------GIREANKLVVPGEDAFFLYDSMGFPIDLTEIMAEEEGMTVDIKGYEECMRLQSERSKMDRKKGGSNGARPLVLEARETSTLAGKHISATDDMAKYEWNIKTPAKVVAIFT---TTESSSDFVDEVKAGDFERVGVILDKTSFYSQAGGQIYDTGVLS--AANFKLDVDSVESYAGYVMHMGPIASGSIKVGDSVECQVDYARRAKIAPNHTMTHALNFALRKVL-------GTVVDQRGSLVDESRLRFDFTNNKALKPNQLAEVEAICDDIIKQQLEVYTQNSAQAEAKRIQGLRAVFGETYPDFVRVVSIGQPIAPMLEDPENSNWSNFSVEFCGGTHLKNTKEAKKFVLYEEGAIAKGIRRVSAYTCDLAVEAEERGAKLQAELDAIDKLDGNEFVESVSAFKPVLDQALISLPLKDKLRKQVDGLVTRVKKIKKEAAAARAANGVRDATAEATKAKEAGQEIVIVKFDVGTDSKLGREMLEAMSTIIPTGSFMIFSTDSDANKTAAFTQVSQHHVDTKQLDARKWVNHAMAVMKGKGGGKD---ALNATGQAKTVEKVDEAVTLAKSF 992