NO28G01320, NO28G01320 (gene) Nannochloropsis oceanica

Overview
NameNO28G01320
Unique NameNO28G01320
Typegene
OrganismNannochloropsis oceanica (N. oceanica IMET1)
Sequence length3064
Alignment locationchr28:415932..418995 -

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Properties
Property NameValue
DescriptionPeptidyl-trna hydrolase pth2 domain-containing protein
Mutants
Expression

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
chr28genomechr28:415932..418995 -
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
PRJNA7699582024-08-13
GSE1786722023-12-15
PRJNA9336932023-10-26
PRJNA9434492023-07-19
GSE1499042020-10-10
NoIMET1_WT_HS2020-09-29
GSE1396152020-03-11
PRJNA2413822020-03-11
BLASTP analysis of N. oceanica IMET1 genes2019-07-11
InterPro analysis for N. oceanica IMET1 genes2019-07-11
GO annotation for IMET1v2 genes2019-07-10
PRJNA1821802019-04-25
Gene prediction for N. oceanica IMET12017-10-24
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004045aminoacyl-tRNA hydrolase activity
GO:0016787hydrolase activity
Vocabulary: INTERPRO
TermDefinition
IPR023476Pep_tRNA_hydro_II_dom
IPR002833PTH2
Vocabulary: Cellular Component
TermDefinition
GO:0016020membrane
Homology
BLAST of NO28G01320 vs. NCBI_GenBank
Match: EWM20852.1 (peptidyl-trna hydrolase pth2 domain-containing protein [Nannochloropsis gaditana])

HSP 1 Score: 209.9 bits (533), Expect = 1.100e-50
Identity = 102/125 (81.60%), Postives = 114/125 (91.20%), Query Frame = 0
Query:  190 IRDFSLLSGPFKMVLCVNMELSMGKGKIAAQCGHATLGAYKRAARHAPTTLRSWEYVGQAKVCLKVPTGADLQQLRALATRAGLVSYQVEDAGRTQIAAGSRTVLAIGPAPVKELDNITGHLKLL 315
            IRDFSL+SGPFKMVLCVNMELSMGKGKIAAQCGHATLGAYKRA R AP TL+ WEY+GQAK+CLKVP   +L++LR L T+A L+SYQVEDAGRTQIAAGS+TVLAIGPAPV+E+D ITGHLKLL
Sbjct:   91 IRDFSLVSGPFKMVLCVNMELSMGKGKIAAQCGHATLGAYKRARRLAPRTLQGWEYMGQAKICLKVPDQVELERLRVLVTKAQLISYQVEDAGRTQIAAGSQTVLAIGPAPVQEIDRITGHLKLL 215          
BLAST of NO28G01320 vs. NCBI_GenBank
Match: CBJ32052.1 (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 159.8 bits (403), Expect = 1.300e-35
Identity = 77/126 (61.11%), Postives = 96/126 (76.19%), Query Frame = 0
Query:  189 GIRDFSLLSGPFKMVLCVNMELSMGKGKIAAQCGHATLGAYKRAARHAPTTLRSWEYVGQAKVCLKVPTGADLQQLRALATRAGLVSYQVEDAGRTQIAAGSRTVLAIGPAPVKELDNITGHLKLL 315
            G R +SL  GPFK+VL VNM+L MGKGK+ AQC HATLG Y+ + RH P+ LR WE++GQAK+C+K PT  +L  ++A A  AGLV+Y V DAG TQIAAGSRTVLA+GPAPV   + I+ HLKL+
Sbjct:   37 GARAWSLDDGPFKLVLIVNMQLKMGKGKVVAQCCHATLGNYRISERHCPSALRGWEHMGQAKICVKCPTETELYDIQAKAQSAGLVNYLVMDAGHTQIAAGSRTVLALGPAPVWAFEGISSHLKLM 162          
BLAST of NO28G01320 vs. NCBI_GenBank
Match: XP_008897238.1 (peptidyl-tRNA hydrolase [Phytophthora parasitica INRA-310] >ETN17704.1 peptidyl-tRNA hydrolase [Phytophthora parasitica INRA-310] >KUF92938.1 Mitogen-activated protein kinase kinase 1 [Phytophthora nicotianae] >KUG02300.1 peptidyl-tRNA hydrolase 2 [Phytophthora nicotianae])

HSP 1 Score: 150.6 bits (379), Expect = 8.100e-33
Identity = 73/116 (62.93%), Postives = 89/116 (76.72%), Query Frame = 0
Query:  199 PFKMVLCVNMELSMGKGKIAAQCGHATLGAYKRAARHAPTTLRSWEYVGQAKVCLKVPTGADLQQLRALATRAGLVSYQVEDAGRTQIAAGSRTVLAIGPAPVKELDNITGHLKLL 315
            P KMV+CV  +L MGKGK+AAQC HATLGAYKRA +  P  +R+WE +GQAKVCLKV +  ++  L   A   GLV+Y V DAGRTQIA  S+TVL+IGPAP+KE+D ITGHLKL+
Sbjct:   77 PHKMVMCVRTDLKMGKGKVAAQCCHATLGAYKRALKRTPDAVRAWEMLGQAKVCLKVDSEEEMLALAEKAAEHGLVNYVVVDAGRTQIAPESKTVLSIGPAPLKEIDEITGHLKLM 192          
BLAST of NO28G01320 vs. NCBI_GenBank
Match: OQS04825.1 (peptidyl-tRNA hydrolase [Thraustotheca clavata])

HSP 1 Score: 150.2 bits (378), Expect = 1.100e-32
Identity = 77/121 (63.64%), Postives = 89/121 (73.55%), Query Frame = 0
Query:  194 SLLSGPFKMVLCVNMELSMGKGKIAAQCGHATLGAYKRAARHAPTTLRSWEYVGQAKVCLKVPTGADLQQLRALATRAGLVSYQVEDAGRTQIAAGSRTVLAIGPAPVKELDNITGHLKLL 315
            SL   P KMVL V  +L MGKGKIAAQCGHATLGAYKRA +  P  L  WE +GQAKV LKV T AD+ +L A A   GLV Y V DAGRTQIA  ++TVLA+GPAP  ++DN+TGHLKL+
Sbjct:   57 SLKWEPHKMVLVVRNDLKMGKGKIAAQCGHATLGAYKRAVKRTPQALECWEALGQAKVALKVETEADMLELAARAKELGLVHYVVVDAGRTQIAPDTKTVLAVGPAPNGDIDNLTGHLKLM 177          
BLAST of NO28G01320 vs. NCBI_GenBank
Match: XP_009517234.1 (hypothetical protein PHYSODRAFT_344137 [Phytophthora sojae] >EGZ29959.1 hypothetical protein PHYSODRAFT_344137 [Phytophthora sojae])

HSP 1 Score: 149.1 bits (375), Expect = 2.400e-32
Identity = 72/116 (62.07%), Postives = 88/116 (75.86%), Query Frame = 0
Query:  199 PFKMVLCVNMELSMGKGKIAAQCGHATLGAYKRAARHAPTTLRSWEYVGQAKVCLKVPTGADLQQLRALATRAGLVSYQVEDAGRTQIAAGSRTVLAIGPAPVKELDNITGHLKLL 315
            P KMV+CV  +L MGKGKIAAQC HATLGAYKRA +  P  +R+WE +GQAKVCLKV +  ++  L   A   GLV+Y V DAGRTQIA  S+TVL+IGPAP+K +D +TGHLKL+
Sbjct:   75 PHKMVMCVRTDLKMGKGKIAAQCCHATLGAYKRAVKRTPDAVRAWEMLGQAKVCLKVDSEEEMLALAERAAEHGLVNYVVVDAGRTQIAPDSKTVLSIGPAPLKAIDEVTGHLKLM 190          
BLAST of NO28G01320 vs. NCBI_GenBank
Match: POM67434.1 (Peptidyl-tRNA hydrolase [Phytophthora palmivora var. palmivora])

HSP 1 Score: 149.1 bits (375), Expect = 2.400e-32
Identity = 74/116 (63.79%), Postives = 87/116 (75.00%), Query Frame = 0
Query:  199 PFKMVLCVNMELSMGKGKIAAQCGHATLGAYKRAARHAPTTLRSWEYVGQAKVCLKVPTGADLQQLRALATRAGLVSYQVEDAGRTQIAAGSRTVLAIGPAPVKELDNITGHLKLL 315
            P KMVLCV  +L MGKGKIAAQC HATLGAYKRA +  P  +R+WE +GQAKVCLKV +  ++  L   A   GLV+Y V DAGRTQIA  S+TVL IGPAP+K +D ITGHLKL+
Sbjct:   70 PHKMVLCVRTDLKMGKGKIAAQCCHATLGAYKRALKRTPDAVRAWEMLGQAKVCLKVDSEEEMLLLAEKAAERGLVNYVVVDAGRTQIAPESKTVLGIGPAPLKAIDEITGHLKLM 185          
BLAST of NO28G01320 vs. NCBI_GenBank
Match: GAX96580.1 (peptidyl-tRNA hydrolase, putative [Pythium insidiosum])

HSP 1 Score: 148.7 bits (374), Expect = 3.100e-32
Identity = 70/116 (60.34%), Postives = 87/116 (75.00%), Query Frame = 0
Query:  199 PFKMVLCVNMELSMGKGKIAAQCGHATLGAYKRAARHAPTTLRSWEYVGQAKVCLKVPTGADLQQLRALATRAGLVSYQVEDAGRTQIAAGSRTVLAIGPAPVKELDNITGHLKLL 315
            P KMVLCV  +L MGKGKIAAQC HATLGAYKRA +  P  +++WE +GQAKVCLKV +  ++  L   AT  GL++Y V DAGRTQIA  S+TVL IGPAP+  ++ +TGHLKL+
Sbjct:   85 PHKMVLCVRTDLKMGKGKIAAQCCHATLGAYKRAVKRTPNAVKAWEMLGQAKVCLKVDSEEEMMSLAEAATACGLINYVVVDAGRTQIAPDSKTVLGIGPAPIDAINEVTGHLKLM 200          
BLAST of NO28G01320 vs. NCBI_GenBank
Match: EJK65910.1 (hypothetical protein THAOC_13191 [Thalassiosira oceanica])

HSP 1 Score: 148.3 bits (373), Expect = 4.000e-32
Identity = 70/122 (57.38%), Postives = 88/122 (72.13%), Query Frame = 0
Query:  193 FSLLSGPFKMVLCVNMELSMGKGKIAAQCGHATLGAYKRAARHAPTTLRSWEYVGQAKVCLKVPTGADLQQLRALATRAGLVSYQVEDAGRTQIAAGSRTVLAIGPAPVKELDNITGHLKLL 315
            + +   P+KMVLCVN  LSMGKGKIAAQCGHA +G YKRA RH    L +WE  G AK+ +K P+  ++ ++ A A   G+  Y VEDAGRTQIAAGSRTVL +GPAPV+E D ++ HLKL+
Sbjct:   65 WGISDAPYKMVLCVNTSLSMGKGKIAAQCGHAAVGCYKRAKRHCQAGLSAWERTGCAKIAVKCPSEDEMDEIAAKALSRGIPIYLVEDAGRTQIAAGSRTVLGLGPAPVREFDGVSNHLKLM 186          
BLAST of NO28G01320 vs. NCBI_GenBank
Match: ETI34089.1 (peptidyl-tRNA hydrolase [Phytophthora parasitica P1569] >ETK74435.1 peptidyl-tRNA hydrolase [Phytophthora parasitica] >ETL27856.1 peptidyl-tRNA hydrolase [Phytophthora parasitica] >ETM34297.1 peptidyl-tRNA hydrolase [Phytophthora parasitica])

HSP 1 Score: 148.3 bits (373), Expect = 4.000e-32
Identity = 72/116 (62.07%), Postives = 88/116 (75.86%), Query Frame = 0
Query:  199 PFKMVLCVNMELSMGKGKIAAQCGHATLGAYKRAARHAPTTLRSWEYVGQAKVCLKVPTGADLQQLRALATRAGLVSYQVEDAGRTQIAAGSRTVLAIGPAPVKELDNITGHLKLL 315
            P KMV+CV  +L MGKGK+AAQC HATLGAYKRA +  P  +R+WE +GQAKVCLKV +  ++  L   A   GLV+Y V DAGRTQIA  S+TVL+IGPAP+K +D ITGHLKL+
Sbjct:   77 PHKMVMCVRTDLKMGKGKVAAQCCHATLGAYKRALKRTPDAVRAWEMLGQAKVCLKVDSEEEMLALAEKAAEHGLVNYVVVDAGRTQIAPESKTVLSIGPAPLKAIDEITGHLKLM 192          
BLAST of NO28G01320 vs. NCBI_GenBank
Match: ETL81108.1 (peptidyl-tRNA hydrolase [Phytophthora parasitica] >ETO62894.1 peptidyl-tRNA hydrolase [Phytophthora parasitica P1976] >ETP03959.1 peptidyl-tRNA hydrolase [Phytophthora parasitica CJ01A1] >ETP32114.1 peptidyl-tRNA hydrolase [Phytophthora parasitica P10297])

HSP 1 Score: 148.3 bits (373), Expect = 4.000e-32
Identity = 72/116 (62.07%), Postives = 88/116 (75.86%), Query Frame = 0
Query:  199 PFKMVLCVNMELSMGKGKIAAQCGHATLGAYKRAARHAPTTLRSWEYVGQAKVCLKVPTGADLQQLRALATRAGLVSYQVEDAGRTQIAAGSRTVLAIGPAPVKELDNITGHLKLL 315
            P KMV+CV  +L MGKGK+AAQC HATLGAYKRA +  P  +R+WE +GQAKVCLKV +  ++  L   A   GLV+Y V DAGRTQIA  S+TVL+IGPAP+K +D ITGHLKL+
Sbjct:   77 PHKMVMCVRTDLKMGKGKVAAQCCHATLGAYKRALKRTPDAVRAWEMLGQAKVCLKVDSEEEMLALAEKAAEHGLVNYVVVDAGRTQIAPESKTVLSIGPAPLKAIDEITGHLKLM 192          
The following BLAST results are available for this feature:
BLAST of NO28G01320 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes)
Total hits: 20
Match NameE-valueIdentityDescription
EWM20852.11.100e-5081.60peptidyl-trna hydrolase pth2 domain-containing pro... [more]
CBJ32052.11.300e-3561.11conserved unknown protein [Ectocarpus siliculosus][more]
XP_008897238.18.100e-3362.93peptidyl-tRNA hydrolase [Phytophthora parasitica I... [more]
OQS04825.11.100e-3263.64peptidyl-tRNA hydrolase [Thraustotheca clavata][more]
XP_009517234.12.400e-3262.07hypothetical protein PHYSODRAFT_344137 [Phytophtho... [more]
POM67434.12.400e-3263.79Peptidyl-tRNA hydrolase [Phytophthora palmivora va... [more]
GAX96580.13.100e-3260.34peptidyl-tRNA hydrolase, putative [Pythium insidio... [more]
EJK65910.14.000e-3257.38hypothetical protein THAOC_13191 [Thalassiosira oc... [more]
ETI34089.14.000e-3262.07peptidyl-tRNA hydrolase [Phytophthora parasitica P... [more]
ETL81108.14.000e-3262.07peptidyl-tRNA hydrolase [Phytophthora parasitica] ... [more]

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Relationships

This gene is member of the following syntenic_region feature(s):

Feature NameUnique NameSpeciesType
ncniR065ncniR065Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ngnoR094ngnoR094Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region


The following polypeptide feature(s) derives from this gene:

Feature NameUnique NameSpeciesType
NO28G01320.1NO28G01320.1-proteinNannochloropsis oceanica (N. oceanica IMET1)polypeptide


The following gene feature(s) are orthologous to this gene:

Feature NameUnique NameSpeciesType
jgi.p|Nanoce1779_2|609390gene_9863Nannochloropsis oceanica (N. oceanica CCMP1779)gene
Naga_100514g3gene9566Nannochloropsis gaditana (N. gaditana B-31)gene


The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
NO28G01320.1NO28G01320.1Nannochloropsis oceanica (N. oceanica IMET1)mRNA


Sequences
The following sequences are available for this feature:

gene sequence

>NO28G01320 ID=NO28G01320|Name=NO28G01320|organism=Nannochloropsis oceanica|type=gene|length=3064bp
ATGGGTGGGATGACATACCTTCGGAGGGGGAGCAGCAGCAGCAGCAGCAG
CAGCAGTTTTTCTGTCAAAGAATGCAAATGTAGCATTTGGAGAAGCTACA
ACTACTGCTGTCATTGGTGGATAAgtgagtcttcacataacgtcgaagaa
ggaaagtggtgaaaggcttttggaatgatgcaagaagtgaggacaaaaac
atgatctcgaagcaatcacaagcgtggagtaaaatagcaatgaagaaata
cgcactgtggcaggagatgggaggtaattgccttcgaatggcgtatttta
tgctctgcttggtctcgtatgtcggcatgtatgtatggtacatacgtaag
cggatgcgcgacagtgttgcacagcaacatagcccccgttcccaaaatcc
agggattgtttaaaacaatattaccagcctctagctctctgtaggcaaat
ggggaggccttctaaaaccccgcaaaaggcccgtagggacgtacaggggg
aggtttttttatcgaaagatgagacgcattccccgttccccccaattcgt
aactgtttggtctgcaaatgatgtcgatggccatacgtgtagccattata
attcaatcaattaattaaatacgtagatgccctataggcttgaccgtata
ggcttgaccatggtcagtgtcatcgactctgagatccctaaccccccaaa
aaaattgacgagtacatgaccacaaatacataaagaacaatttcggaggc
ttttttgctgcaaaaagtgcccgtaggactcggttgggcttggtacaggt
gcatatgggtgtgcgtcaggtgccttttgctttttacagggtttgctctg
cttctcaaacccctcaaaaagcatcgaacacacatcgacaaacccctgaa
aaagcaagcgagggttttttgctttttgaggggttggatggcgaacacac
ccactactcaactgaagcgcaacggacacggacgcgcaaactttgaaact
tcttactacctcctcttgagcagTGGACGGCAAGCCTAGCTTGCATATCG
CAAAAGCTCACGACCGGAAATCCCCTCATCCAACAAAATTGCCTTACAGT
ACAGCAGCAGCAGCAACAGTAGCAGCAGCAGCAGCGGTAGCCGCAGCAGT
AGCAGCAGTAGCAGCAGTAGCAGCAGGAGGCAATCATCATGGCATTCGCT
GCGTACTCATCCGTTCTTGCGGCCGTTTTAGAGGCCGCGATTGCAACGCC
CGGGCGGGCTCTCCTTCTTTCCTTCGCCGCGGGCATGTTGGTAACGGGGT
GTGTGCTGCCCATGGGGGGGGGAGGAGGCGGCGCCGACGCCGTCTACAAC
CCTTGGAGGAACGAAAGGAACAGGAGGTAGGGGACGGGGAGGACGACGAC
GAGCAAGAAAGCGGGAGTGAGAGCGAAAGCGACGAAGACAGTGAAACCGC
AGCGGCCAGCGGGGGCATTCGTGACTTCTCCCTTCTGTCTGGTCCCTTCA
AGATGGTGCTCTGTGTCAACATGGAACTGTCGATGGGAAAAGGCAAGATT
GCTGCACAATGCGGTCACGCCACCCTAGGTGCTTACAAACGTGCAGCGCG
GCATGCCCCCACTACATTGCGAAGTTGGGAGTATGTTGGgtgagtgtggg
atggaggaagggaaggcaagatgaagagagggcagggattgtacggatgc
gcgaaagcctgcttgcaagatgctacgtgtggtgtggtagcacagggctt
tatccgccgtattaaaaagaagtcatggtatcggatcgaatgcactaatt
tcaaaatataggtcaggcttccctccagtgtgtggacgtcgatgaccgtt
cgtgttcgctgtgcgactgcaaaagcatggcgccctgacctgtcggagaa
catatgtccatgtgtggatgtatttgtgtaatgatccttcaatgcatcat
attgtccttcctcttttccttccctcagGCAAGCCAAGGTGTGTCTTAAA
GTCCCGACGGGAGCAGACCTGCAACAGCTCCGCGCCTTGGCCACACGGGC
GGGGTTGGTTTCCTACCAGGTGGAAGATGCCGGACGCACGCAGATAGCTG
CAGGATCACGGACTGTCTTAGCGATCGGTCCCGCGCCAGTGAAAGAGCTG
GATAACATCACGGGACACTTGAAACTACTCTGACAGCGCACACAATGAGC
GAATCCTAGTACCATAAAGTATATACGAACGTATTGAAGCATGCCACATG
ACACTTAAGCGTTGAAGGAACAGAATGTCGTTTCGTTCTTATGCTTGGTT
TGCCCGTTCCATTTTTGGTTTTCTCTTCGTTTATCGTTTTTCTTCAAATC
TGTCCTCTATTTTTCACAAAATCTGTGGCATTGTCTTTATGCAATCATCC
GTGTTTCACGGTTCGTCGTGCATATCCCCCCATCCCCCTTTCCTTGTCCG
CTAGGACCATAGGGGACCAGATATTTACATCCTCTTCTTGTTTCCTCTTA
TTGTTTCCTCTTATTGTTCGGACACTCTTTCCGACCGCGGGTGGAGGAGG
GGAGGAAGGGAAGAAAAGAGGGGGAGGGAAGGAGAATGTGCCTCGCCCCT
CCTGTGTCTTCTACCAATGGAACACCTCCTCGTGCATTGCTATCCTCTCT
TTTGCTTCTTGCAACTCCATCACTGCGTTGCGAGCCTCCACAAGCATGGC
ATCCGGGCCACATACCACCACCCCTTTCTCCCCCCCGTCCTCCTTCCCTC
CTTCCCGCGATTCACTCCATTTTTTGACCATCGTTCTCACTTCCGGTCGC
CCACAGTCGTAGACTAATCCTCCTTCCGTCACGCCTCCTCGCCTTCCTTC
CTCCCTCCCTCCCTCCCTCCCGCCCGTCACATACAGCCGAAGGGTGAATG
AGAAGGAAGGAGGGAGGGAGGGCGAGAATTTCGCCCGCACAGATGTCGCG
GCAGCATCAGCAGCAACAGCAGCAACAGCAGAGCGCAACGGCATGCCATT
CTTACTCTCTTCCCCGTTCTCGTCCTCATCCTTCCACTCTCCGCCCCCCT
CCCCCCTCCCTCCC
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protein sequence of NO28G01320.1

>NO28G01320.1-protein ID=NO28G01320.1-protein|Name=NO28G01320.1|organism=Nannochloropsis oceanica|type=polypeptide|length=315bp
MGGMTYLRRGSSSSSSSSSFSVKECKCSIWRSYNYCCHWWIMDGKPSLHI
AKAHDRKSPHPTKLPYSTAAAATVAAAAAVAAAVAAVAAVAAGGNHHGIR
CVLIRSCGRFRGRDCNARAGSPSFLRRGHVGNGVCAAHGGGRRRRRRRLQ
PLEERKEQEVGDGEDDDEQESGSESESDEDSETAAASGGIRDFSLLSGPF
KMVLCVNMELSMGKGKIAAQCGHATLGAYKRAARHAPTTLRSWEYVGQAK
VCLKVPTGADLQQLRALATRAGLVSYQVEDAGRTQIAAGSRTVLAIGPAP
VKELDNITGHLKLL*
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Synonyms
Publications