NO28G00270, NO28G00270 (gene) Nannochloropsis oceanica

Overview
NameNO28G00270
Unique NameNO28G00270
Typegene
OrganismNannochloropsis oceanica (N. oceanica IMET1)
Sequence length1615
Alignment locationchr28:104090..105704 +

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Properties
Property NameValue
Descriptionribonuclease 1
Mutants
Expression

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
chr28genomechr28:104090..105704 +
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
PRJNA7699582024-08-13
GSE1786722023-12-15
PRJNA9336932023-10-26
PRJNA9434492023-07-19
GSE1499042020-10-10
NoIMET1_WT_HS2020-09-29
GSE1396152020-03-11
PRJNA2413822020-03-11
BLASTP analysis of N. oceanica IMET1 genes2019-07-11
InterPro analysis for N. oceanica IMET1 genes2019-07-11
GO annotation for IMET1v2 genes2019-07-10
PRJNA1821802019-04-25
Gene prediction for N. oceanica IMET12017-10-24
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0033897ribonuclease T2 activity
GO:0003723RNA binding
GO:0033897ribonuclease T2 activity
GO:0003723RNA binding
Vocabulary: INTERPRO
TermDefinition
IPR036430RNase_T2-like_sf
IPR001568RNase_T2-like
Vocabulary: Biological Process
TermDefinition
GO:0090502RNA phosphodiester bond hydrolysis, endonucleolytic
Homology
BLAST of NO28G00270 vs. NCBI_GenBank
Match: CBJ26931.1 (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 164.1 bits (414), Expect = 6.000e-37
Identity = 77/208 (37.02%), Postives = 113/208 (54.33%), Query Frame = 0
Query:   50 PKEFDFYLLATFWPAELCHRETTNSCQYPQDWWKVNLTGHGLWPNNNDGTYPTSCGGAPFNDSIPEETIGMPIMTTYWPNFQYDVEDGEELYDNLWEHEWNKHGTCTGLDQYIYMTTTLAGIMN--ATPRLLSQTAXXXXXXXXXXXXXXPLLTLQNAFGGPTMAVLQCTSHKYLTGVFTCWSKNNETNLPYARIPCPAAVL-DEGTC 255
            P +F+ Y+ A  W AE C+ +    C+ P+ +W+ NLT HGLWP+  DGTYPT+C   P++       IG+ ++ T WPN Q   E   + YD+ WEHEW KHGTCTGL    Y +T ++ +     TP  L++                  + L++A+GG  MAVL C    +L  V+ C   + ET+LP  ++ CP AV+  + TC
Sbjct:   85 PGDFNLYIFAMSWKAEWCYHQNFPGCKKPRSFWEKNLTIHGLWPDYGDGTYPTTCSSEPYDHDRVVNAIGLDVLETMWPNIQVPEEFAGKKYDSFWEHEWTKHGTCTGLSMEEYFSTAISLLQRRFLTPPQLAENVGGQTTR----------VDLEDAYGGEGMAVLDCRGKVHLNQVYLCLEPDPETHLPGEQVACPPAVVAHDDTC 282          
BLAST of NO28G00270 vs. NCBI_GenBank
Match: EWM20292.1 (conserved unknown protein, partial [Nannochloropsis gaditana])

HSP 1 Score: 152.9 bits (385), Expect = 1.400e-33
Identity = 70/116 (60.34%), Postives = 82/116 (70.69%), Query Frame = 0
Query:  151 KHGTCTGLDQYIYMTTTLAGIMNATPRLLSQTAXXXXXXXXXXXXXXPLLTLQNAFGGPTMAVLQCTSHKYLTGVFTCWSKNNETNLPYARIPCPAAVLDEGTCQKGGDVVVPLFK 267
            KHGTCTGLDQY YMTTT+AGI  ATP LL + A              PL  L+ AFGGP MAVL C   KYLTGV+TCW+K++ T+ PYAR+ CP AV+ EGTC KGG+VVVP+FK
Sbjct:   23 KHGTCTGLDQYTYMTTTIAGITTATPTLLGEMAATAAAASPPHPPSLPLPDLETAFGGPGMAVLMCNGKKYLTGVYTCWTKDSGTHKPYARMQCPPAVVAEGTCPKGGEVVVPIFK 138          
BLAST of NO28G00270 vs. NCBI_GenBank
Match: GAX99438.1 (Hypothetical protein PINS_007264 [Pythium insidiosum])

HSP 1 Score: 148.7 bits (374), Expect = 2.600e-32
Identity = 74/208 (35.58%), Postives = 106/208 (50.96%), Query Frame = 0
Query:   53 FDFYLLATFWPAELCH--RETTNSCQYPQDWWKVNLTGHGLWPNNNDGTYPTSCGGAPFNDSIPEETIGMPIMTTYWPNFQYDVEDGEELYDNLWEHEWNKHGTCTGLDQYIYMTTTLAGIMN---ATPRLLSQTAXXXXXXXXXXXXXXPLLTLQNAFGGPTMAVLQCTSHKYLTGVFTCWSKNNETNLPYARIPCPAAVLDEGTCQ 256
            FDFY+LA  W  E CH   +    C  P+D+W+ + T HGLWP    G +P  C G  F+ +  EE +G+ ++  +WPN +  VE G   Y + W HEW +HGTC+GL+Q  Y +  +  I N   +TP L+ Q                  +       G    VL+C  + +L   FTCW+K++ TN P  R  CPA +L E TC+
Sbjct:   64 FDFYVLAVSWQPEFCHGKEQEYPGCVKPRDFWRSHFTLHGLWPERERGKHPGFCHGPAFDPAAVEEAVGLDVLLQFWPNVK--VEAGSADYVDFWRHEWTRHGTCSGLEQTTYFSRAVDIICNGTLSTPALVQQNVGKSVSTADLRAAFKRDVDGDEDDDG--NVVLKCDHNNHLAQAFTCWAKDDATNEPTTRRACPAHILSEDTCR 267          
BLAST of NO28G00270 vs. NCBI_GenBank
Match: OQR86380.1 (hypothetical protein ACHHYP_10610 [Achlya hypogyna])

HSP 1 Score: 147.9 bits (372), Expect = 4.500e-32
Identity = 76/214 (35.51%), Postives = 109/214 (50.93%), Query Frame = 0
Query:   53 FDFYLLATFWPAELCHRETTNSCQYPQDWWKVNLTGHGLWPNNNDGTYPTSCGGAPFNDSIPEETIGMPIMTTYWPNFQYDVEDGEELYDNLWEHEWNKHGTCTGLDQYIYMTTTLAGIM-NATPRLLSQTAXXXXXXXXXXXXXXPLLTLQNAFGGPTMAVLQCTSHKYLTGVFTCWSKNNETNLPYARIPCPAAVLDEGTCQKGGDVVVPLF 266
            FDFY+ A  W A  C+ E    C+ P+ +WK + T HGLWP  N G +P  C   P +  +  + IG   +  YWPN +  V      YD+ W HEW +HGTC+GLDQ  +  + +  I  N TP  + +                    L++A+GGPT AVLQC  +K L+ V+TC +K+ + N P     C   VL E  C   G++++P F
Sbjct:   40 FDFYVFAHSWQASFCYHEPYPGCKEPEAYWKTHFTVHGLWPELNKGPHPGFCSKEPLDIKLVRQAIGEDTLEEYWPNVKVAVNTTG--YDSFWNHEWTRHGTCSGLDQVTFFQSAIDLIKDNGTPDFVQKNVGNVVSTK----------ELRDAYGGPTKAVLQC-KNKRLSQVYTCLAKDKD-NKPTEATDCSPQVLKEDNCHV-GEILIPKF 238          
BLAST of NO28G00270 vs. NCBI_GenBank
Match: GAX99751.1 (Hypothetical protein PINS_007583 [Pythium insidiosum])

HSP 1 Score: 146.0 bits (367), Expect = 1.700e-31
Identity = 74/208 (35.58%), Postives = 105/208 (50.48%), Query Frame = 0
Query:   53 FDFYLLATFWPAELCH--RETTNSCQYPQDWWKVNLTGHGLWPNNNDGTYPTSCGGAPFNDSIPEETIGMPIMTTYWPNFQYDVEDGEELYDNLWEHEWNKHGTCTGLDQYIYMTTTLAGIMN---ATPRLLSQTAXXXXXXXXXXXXXXPLLTLQNAFGGPTMAVLQCTSHKYLTGVFTCWSKNNETNLPYARIPCPAAVLDEGTCQ 256
            FDFY+LA  W  E CH   +    C  P+D+W+ + T HGLWP    G +P  C G  F+ +  EE +G+ ++  +WPN +  VE G   Y + W HEW +HGTC+GL+Q  Y +  +    N   +TP L  Q               X  +       G    VL+C  + +L   FTCW+K++ TN P  R  CPA +L E TC+
Sbjct:   64 FDFYVLAVSWQPEFCHGKEQEYPGCVKPRDFWRSHFTLHGLWPERERGKHPGFCHGPAFDPAAVEEAVGLDVLLQFWPNVK--VEAGSADYVDFWRHEWTRHGTCSGLEQTTYFSRAVDIXXNGTLSTPXLXQQNVGKSVSTADLRAAFXRDVDGDEDDDG--NVVLKCDHNNHLAQAFTCWAKDDATNEPTTRRACPAHILSEDTCR 267          
BLAST of NO28G00270 vs. NCBI_GenBank
Match: XP_009838822.1 (hypothetical protein H257_13092 [Aphanomyces astaci] >ETV71634.1 hypothetical protein H257_13092 [Aphanomyces astaci])

HSP 1 Score: 144.4 bits (363), Expect = 4.900e-31
Identity = 75/217 (34.56%), Postives = 107/217 (49.31%), Query Frame = 0
Query:   51 KEFDFYLLATFWPAELCHRETTNSCQYPQDWWKVNLTGHGLWPNNNDGTYPTSCGGAPFNDSIPEETIGMPIMTTYWPNFQYDVEDGEELYDNLWEHEWNKHGTCTGLDQYIYMTTTLAGI-MNATPRLLSQTAXXXXXXXXXXXXXXPLLTLQNAFGGPTMAVLQCTSHKYLTGVFTCWSKNNETNLPYARIPCPAAVLDEGTCQKGGDVVVPLFK 267
            K+FDFY+ A  W    C       C  P+ +W+ + T HGLWP   DG +P  C   P + +I    IG   +  YWPN +  V      YD+ W HEW++HGTC+GLDQ  +  + +  I +  TP  ++Q                    +++AFGG   AVL+C     L+ VFTC+ K+  +N+P     C A VL E TC+    VV+  FK
Sbjct:   38 KDFDFYVFAHSWQPSFCAGTDFPGCWVPELYWESHFTIHGLWPELEDGPHPGFCTKEPLDINIVRSAIGEDTLEKYWPNVK--VAANTTHYDSFWGHEWSRHGTCSGLDQVEFFQSAIDKIKVQGTPAFVTQHVGQSVSTK----------DVRDAFGGAGQAVLKCEHGNELSQVFTCYDKDASSNVPTTLRACSAHVLAEDTCKSTATVVIRGFK 242          
BLAST of NO28G00270 vs. NCBI_GenBank
Match: AIG55452.1 (secreted protein [Thraustotheca clavata] >OQS05596.1 hypothetical protein THRCLA_02301 [Thraustotheca clavata])

HSP 1 Score: 143.7 bits (361), Expect = 8.400e-31
Identity = 77/220 (35.00%), Postives = 108/220 (49.09%), Query Frame = 0
Query:   52 EFDFYLLATFWPAELCHRE-----TTNSCQYPQDWWKVNLTGHGLWPNNNDGTYPTSCGGAPFNDSIPEETIGMPIMTTYWPNFQYDVEDGEELYDNLWEHEWNKHGTCTGLDQYIYMTTTLAGI-MNATPRLLSQTAXXXXXXXXXXXXXXPLLTLQNAFGGPTMAVLQCTSHKYLTGVFTCWSKNNETNLPYARIPCPAAVLDEGTCQKGGDVVVPLF 266
            +FDFY+ A  W    C+ E         C+ PQ++WK + T HGLWP  N+G +P  C     N  +  + IG   +  YWPN +  V      YD+ W HEW +HGTC+GLDQ  Y  + +  I  + TP  + ++                   L+ A+GGP   VLQC   K L+ VFTC +K ++ N+P     C   VL E TC  G +V +P F
Sbjct:   39 QFDFYVFAHSWQPSFCYHEPYPAQALLGCKEPQEYWKTHFTVHGLWPELNEGPHPGFCSKEELNIDLVRKAIGEVTLEEYWPNVKVPVNSSS--YDSFWNHEWTRHGTCSGLDQVTYFKSAIELIKTHGTPECIQKSVGKTMSTK----------ELREAYGGPKRVVLQCKG-KTLSQVFTCIAK-DKNNVPTKASDCSIQVLKEDTCH-GTEVYLPAF 243          
BLAST of NO28G00270 vs. NCBI_GenBank
Match: XP_008617861.1 (hypothetical protein SDRG_13546 [Saprolegnia diclina VS20] >EQC28669.1 hypothetical protein SDRG_13546 [Saprolegnia diclina VS20])

HSP 1 Score: 142.9 bits (359), Expect = 1.400e-30
Identity = 69/215 (32.09%), Postives = 110/215 (51.16%), Query Frame = 0
Query:   52 EFDFYLLATFWPAELCHRETTNSCQYPQDWWKVNLTGHGLWPNNNDGTYPTSCGGAPFNDSIPEETIGMPIMTTYWPNFQYDVEDGEELYDNLWEHEWNKHGTCTGLDQYIYMTTTLAGI-MNATPRLLSQTAXXXXXXXXXXXXXXPLLTLQNAFGGPTMAVLQCTSHKYLTGVFTCWSKNNETNLPYARIPCPAAVLDEGTCQKGGDVVVPLF 266
            +FD+Y+ A  W A  C++E    C+ P  +WK + T HGLWP  N+G +P  C   P +     + IG   +  +WPN +  +      YD+ W HEW +HGTC+GLDQ  +  + +  +    TP  +SQ+                   +++A+GGP   VLQC + + L+ V+TC  K+ + N P     C + VL E  C + G++++P F
Sbjct:   35 QFDYYVFAHSWQASFCYKEPYPGCKAPNPYWKTHFTVHGLWPELNEGPHPGYCSKEPLDMQRVRKAIGQDTLEKFWPNVKVAINTTG--YDSFWNHEWTRHGTCSGLDQVTFFQSAIERMKTQGTPDFISQSVGKVVATS----------AIRDAYGGPKKVVLQCKNQR-LSQVYTCLGKDKD-NKPTDATECSSQVLKEDNC-RVGEILIPAF 234          
BLAST of NO28G00270 vs. NCBI_GenBank
Match: XP_012202475.1 (ribonuclease T2 family protein [Saprolegnia parasitica CBS 223.65] >KDO26829.1 ribonuclease T2 family protein [Saprolegnia parasitica CBS 223.65])

HSP 1 Score: 138.3 bits (347), Expect = 3.500e-29
Identity = 66/215 (30.70%), Postives = 110/215 (51.16%), Query Frame = 0
Query:   52 EFDFYLLATFWPAELCHRETTNSCQYPQDWWKVNLTGHGLWPNNNDGTYPTSCGGAPFNDSIPEETIGMPIMTTYWPNFQYDVEDGEELYDNLWEHEWNKHGTCTGLDQYIYMTTTLAGI-MNATPRLLSQTAXXXXXXXXXXXXXXPLLTLQNAFGGPTMAVLQCTSHKYLTGVFTCWSKNNETNLPYARIPCPAAVLDEGTCQKGGDVVVPLF 266
            +FD+Y+ A  W    C+++    C+ P  +WK + T HGLWP  N+G +P  C   P +     + IG   +  +WPN +  +   +  YD+ W HEW +HGTC+GLDQ  +  + +  +    TP  +S++                   +++A+GGP   VLQC + + L+ V+TC  K+ + N P     C   VL E +C + G++++P F
Sbjct:   35 QFDYYVFAHSWQPSFCYQQPYPGCKEPNPYWKTHFTVHGLWPEKNEGPHPGYCTKEPLDMQRVRKAIGQDTLEKFWPNVKVAINTTD--YDSFWNHEWTRHGTCSGLDQVTFFQSAIERMKTQGTPDFISKSVGKVVSTS----------AIRDAYGGPKKVVLQCKNQR-LSQVYTCLGKDKD-NQPTNATDCSRQVLKEDSC-RVGEILIPGF 234          
BLAST of NO28G00270 vs. NCBI_GenBank
Match: XP_008869363.1 (hypothetical protein H310_06179 [Aphanomyces invadans] >ETW01515.1 hypothetical protein H310_06179 [Aphanomyces invadans])

HSP 1 Score: 137.1 bits (344), Expect = 7.900e-29
Identity = 72/218 (33.03%), Postives = 102/218 (46.79%), Query Frame = 0
Query:   49 APKEFDFYLLATFWPAELCHRETTNSCQYPQDWWKVNLTGHGLWPNNNDGTYPTSCGGAPFNDSIPEETIGMPIMTTYWPNFQYDVEDGEELYDNLWEHEWNKHGTCTGLDQYIYMTTTLAGIMN-ATPRLLSQTAXXXXXXXXXXXXXXPLLTLQNAFGGPTMAVLQCTSHKYLTGVFTCWSKNNETNLPYARIPCPAAVLDEGTCQKGGDVVVPLF 266
            A  +FDFY+ A  W    C  E    C  P  +W  + T HGLWP   +G +P  C   P + +     IG  ++  YWPN +  V      YD+ W HEW++HGTC GLDQ  +  + +  I +  TP  ++Q                    +++AFGG   AVLQC +   L+ VFTC+ K+  +N+P     C   VL E TC     V +  F
Sbjct:   36 AMTDFDFYVFAHSWQPSFCAGEDYPGCSVPDSYWASHFTIHGLWPELANGPHPGFCTKEPLDINSVRTAIGEGVLEQYWPNVK--VAANTTHYDSFWAHEWSRHGTCRGLDQVAFFQSAIDKIKSQGTPDFITQHVGQTVSTK----------DVRDAFGGVNHAVLQCENGNELSQVFTCYDKDVNSNVPTTLRACAPHVLAEDTCTSKSTVFIRSF 241          
The following BLAST results are available for this feature:
BLAST of NO28G00270 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes)
Total hits: 20
Match NameE-valueIdentityDescription
CBJ26931.16.000e-3737.02conserved unknown protein [Ectocarpus siliculosus][more]
EWM20292.11.400e-3360.34conserved unknown protein, partial [Nannochloropsi... [more]
GAX99438.12.600e-3235.58Hypothetical protein PINS_007264 [Pythium insidios... [more]
OQR86380.14.500e-3235.51hypothetical protein ACHHYP_10610 [Achlya hypogyna... [more]
GAX99751.11.700e-3135.58Hypothetical protein PINS_007583 [Pythium insidios... [more]
XP_009838822.14.900e-3134.56hypothetical protein H257_13092 [Aphanomyces astac... [more]
AIG55452.18.400e-3135.00secreted protein [Thraustotheca clavata] >OQS05596... [more]
XP_008617861.11.400e-3032.09hypothetical protein SDRG_13546 [Saprolegnia dicli... [more]
XP_012202475.13.500e-2930.70ribonuclease T2 family protein [Saprolegnia parasi... [more]
XP_008869363.17.900e-2933.03hypothetical protein H310_06179 [Aphanomyces invad... [more]

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Relationships

This gene is member of the following syntenic_region feature(s):

Feature NameUnique NameSpeciesType
ncniR002ncniR002Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region


The following polypeptide feature(s) derives from this gene:

Feature NameUnique NameSpeciesType
NO28G00270.1NO28G00270.1-proteinNannochloropsis oceanica (N. oceanica IMET1)polypeptide


The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
NO28G00270.1NO28G00270.1Nannochloropsis oceanica (N. oceanica IMET1)mRNA


Sequences
The following sequences are available for this feature:

gene sequence

>NO28G00270 ID=NO28G00270|Name=NO28G00270|organism=Nannochloropsis oceanica|type=gene|length=1615bp
ATGCCTTCATCGTCAGCGTTAGCAAGGGGCAGCAGTGGCAGTCACGTACA
AAAAGgtgggcatgatttcccgcccttcctccctttcctccctccttccc
gtccacctctcatacgcacacatcccacacatatacagCCCGCGTCCTCC
CACTCCTTTTGCTGCTCCTATGGACGCCGTTCCCGGTGGTGGACGGCAAA
CGACGTGCCTTGCGCGCCGGATTGCGTGCGCCCAAGGAGTTTGATTTCTA
TCTACTGGCTACCTTCgtacgtgtagagaggaggaaaggaggaaggaaga
ggaagatttaagactgagggattgctataatttaaaatgcatcaatcgaa
taggatattgttatcgtgtataccgtggctttttgagccattccactcac
cttccctctcgccttccctccctcccttccctcccttcctcccttcctcc
ttcccccccgcctccctcccttcctcttagTGGCCCGCAGAATTGTGCCA
CCGGGAGACGACCAACAGCTGTCAATACCCGCAGGATTGGTGGAAGgtgc
gtactcaaagaatagggaggaagggaagaggggacggcagccatcgatag
aatgatcgagcatttctggtgtctcgaggcttacttccttcctccctctc
tccctccctcccttccttcctccatccattcatccctcacagGTCAACCT
GACCGGCCACGGCCTGTGGCCCAACAACAACGACGGTACCTACCCTACCA
GCTGCGGCGGCGCCCCCTTCAATGACTCCATTCCCGAGGAGACGATTGGC
ATGCCCATTATGACTACGTgtgagcaagccttccctccctccctccatcc
ctccctccctcgctctctcttcgcgctcggtctaggcccactttctgctc
ctccaatgatcctcctcccctcccttccttcctcccttccttcctccctc
tctccctcccttccttcctcccttctttcctccctccctccctccagACT
GGCCTAATTTTCAATACGACGTGGAAGATGGAGAAGAGCTCTACGATAAT
CTCTGGGAACACGAGTGgtatgtatgtcctctccctttcccccttccttc
cctccctccctcccttcttccttccccctcgtccctttccctcacaggag
ctttttggattgactggttgatcgagagattgatcctattgtgagaccga
tgtacgatatctacacatagccttttccctccctcccaccctccttcccc
tccctcgtagGAACAAACACGGGACTTGCACCGGACTGGACCAATACATC
TACATGACCACCACCCTTGCAGGCATCATGAATGCCACCCCTCGATTACT
CTCTCAAACCGCTGCTGCTGCTGCTGCTGCTCCTGGAAGTCCCTCTCCCT
CCCTCCCCCTCTTGACCTTGCAAAATGCGTTTGGTGGTCCCACCATGGCC
GTGCTTCAATGCACCAGTCATAAATATTTGACAGGTGTATTCACCTGCTG
GTCCAAGAACAATGAGACGAACTTGCCATACGCGCGCATTCCCTGTCCAG
CAGCGGTCCTGGACGAGGGCACGTGCCAGAAGGGCGGTGATGTGGTAGTA
CCCTTATTTAAGTGA
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protein sequence of NO28G00270.1

>NO28G00270.1-protein ID=NO28G00270.1-protein|Name=NO28G00270.1|organism=Nannochloropsis oceanica|type=polypeptide|length=267bp
MPSSSALARGSSGSHVQKARVLPLLLLLLWTPFPVVDGKRRALRAGLRAP
KEFDFYLLATFWPAELCHRETTNSCQYPQDWWKVNLTGHGLWPNNNDGTY
PTSCGGAPFNDSIPEETIGMPIMTTYWPNFQYDVEDGEELYDNLWEHEWN
KHGTCTGLDQYIYMTTTLAGIMNATPRLLSQTAAAAAAAPGSPSPSLPLL
TLQNAFGGPTMAVLQCTSHKYLTGVFTCWSKNNETNLPYARIPCPAAVLD
EGTCQKGGDVVVPLFK*
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Synonyms
Publications