NO25G00730, NO25G00730 (gene) Nannochloropsis oceanica

BLAST of NO25G00730 vs. NCBI_GenBank
Match: XP_005854208.1 (long-chain acyl-CoA ligase [Nannochloropsis gaditana CCMP526] >EKU22147.1 long-chain acyl-CoA ligase [Nannochloropsis gaditana CCMP526] >EWM21593.1 amp-dependent synthetase and ligase [Nannochloropsis gaditana])

HSP 1 Score: 946.8 bits (2446), Expect = 3.300e-272
Identity = 477/621 (76.81%), Postives = 529/621 (85.19%), Query Frame = 0

Query:    1 MVDKAKYLLQQAGIGPGDKVAVISRNRVEWAVGCMASLSLGATWVPMYEEQRMNECEYILKDSGAKLLLVGSEETYDKTRHFLASNXXXXXXXXXXXXXXXXXXXQQGFQGPVPSLQDIWTFDIDVPARMAMVSPSMLASSP---SSPHIHADDVAVLIYTSGTTGTPKGVELTHANLCSNIQGMWEVVKPEDMPDALSLAFLPWAHSIGLNCELFNMMSRGARVALAQDIKTIASDIKEVKPNILFAVPTLYKKVYDKIQDKINSSGRWSRWLVDTAVAVAEKRLQVLREGGVLSLVLQLQYNLVDRIVLSKLRETMGGNVKLSPVGGSKMSLPVTRFFEAALGIAILEGYGLSETSPLLSISRPEVQTRRLGSVGQAVKGAELAVVRDGRRVPEGEEGEVWAAGPMVFKGYLSKEEETAAAFGELEGRKWFRTGDLGRLSEGFLTITGRLKELYKLENGKYVCAPVVEKALLESEYISQVVLHGADLPFNVALIVPNWEKLTLWALSSKLAGLSPTPSKEELSAHPEVQAFMRQTIVLACKDKVKKYEMPQEWLLLTEAFTVENEMLTPKMSIKRHAVIKAYLPALTALYEQAAIAHRSKSRTKETVANQQPCDDALAA 619
            MV KAK++L++AGIGPGDKVA +S NRVEWAVGCMASLSLGATWVPMYE+QR+ ECEYIL+DSGAKLLLVGS E YDKTRHFL                       +     V SL+DIWTFD+DVPARMA V+  M A +    +S  +  DDVAVLIYTSGTTGTPKGVELTH NLC+NI+GMWEVV+PEDMP+ALSLAFLPWAHSIG+NCELFNMMSRGARVALAQD+ TIASDIKEV+PNILFAVPTLYKKVYD+IQDKI S G+WSRWLVD AVAVAEKRL++LREG  LSL LQ QY ++D IVLSKLR+T+GG V++SPVGGSKMSLPVTRFFEAALGIAILEGYGLSETSPLLSISRP++Q RRLGSVGQAVKGAELAVVRDG RVPEGEEGE+WA+GPMVFKGYL K+EETAAAFGE++G+KWFRTGDLG L +GFLTITGRLKELYKLENGKYVCAPVVEKALLESEYI QVVL+GADLPFN ALIVPNWEKLT W +S KL GLS +  K E+  HPEVQAFMR+ IVLACKDK+KKYEMPQ+WLLL E FTVENEMLTPKMSIKRHAVIKAY  AL A+Y+QA +A   KSR KET   QQP +DALAA
Sbjct:   91 MVGKAKFVLREAGIGPGDKVAALSGNRVEWAVGCMASLSLGATWVPMYEQQRLTECEYILRDSGAKLLLVGSREAYDKTRHFLTRPSRNPAFAQGDALSVINMQESK----TVSSLKDIWTFDVDVPARMARVTGVMTAEADACFASAVVRPDDVAVLIYTSGTTGTPKGVELTHRNLCTNIEGMWEVVRPEDMPNALSLAFLPWAHSIGMNCELFNMMSRGARVALAQDMTTIASDIKEVRPNILFAVPTLYKKVYDRIQDKITSQGKWSRWLVDKAVAVAEKRLEILREGKRLSLGLQAQYEVLDHIVLSKLRDTLGGRVRISPVGGSKMSLPVTRFFEAALGIAILEGYGLSETSPLLSISRPDIQIRRLGSVGQAVKGAELAVVRDGLRVPEGEEGEIWASGPMVFKGYLKKKEETAAAFGEMDGKKWFRTGDLGTLEQGFLTITGRLKELYKLENGKYVCAPVVEKALLESEYIFQVVLYGADLPFNTALIVPNWEKLTAWCVSKKLPGLSASSRKTEIRTHPEVQAFMRENIVLACKDKMKKYEMPQKWLLLEEPFTVENEMLTPKMSIKRHAVIKAYGSALNAIYDQANVASYPKSRAKETPDTQQPYEDALAA 707          

BLAST of NO25G00730 vs. NCBI_GenBank
Match: CBJ27350.1 (long chain acyl-coA synthetase [Ectocarpus siliculosus])

HSP 1 Score: 422.5 bits (1085), Expect = 2.200e-114
Identity = 246/595 (41.34%), Postives = 352/595 (59.16%), Query Frame = 0

Query:    2 VDKAKYLLQQAGIGPGDKVAVISRNRVEWAVGCMASLSLGATWVPMYEEQRMNECEYILKDSGAKLLLVGSEETYDKTRHFLASNXXXXXXXXXXXXXXXXXXXQQGFQGPVPSLQDIWTFDIDVPARMAMVSPSMLASSPSS--PHIHAD--DVAVLIYTSGTTGTPKGVELTHANLCSNIQGMWEVVKPEDMPDA--LSLAFLPWAHSIGLNCELFNMMSRGARVALAQDIKTIASDIKEVKPNILFAVPTLYKKVYDKIQDKINSSGRWSRWLVDTAVAVAEKRLQVLREGGVLSLVLQLQYNLVDRIVLSKLRETMGGNVKLSPVGGSKMSLPVTRFFEAALGIAILEGYGLSETSPLLSISRPEVQTRRLGSVGQAVKGAELAVVRDGRRVPEGEEGEVWAAGPMVFKGYLSKEEETAAAFGELEGRKWFRTGDLGRLSEG-FLTITGRLKELYKLENGKYVCAPVVEKALLESEYISQVVLHGADLPFNVALIVPNWEKLTLWALSSKLAGLSPTPSKEELSAHPEVQAFMRQTIVLACKDKVKKYEMPQEWLLLTEAFTVENEMLTPKMSIKRHAVIKAYLPALTALY 590
            VD+ +  L   GI  GD V +IS NR EWAV   A+ SLGA +VPMYEEQR  +  YIL+DSGAK L V ++  Y +T HF                         G QG V ++     F  D PA        ++ ++     P    D  ++A LIYTSGTTG PKGV L+H N+ SNI G+   + P+D+      SL+FLPWAH  G   EL  +M+ GA + +++D+  +   + EVKP +L+AVP L+K+V D I  K+  S    ++L   ++ +A +R + +  G  +   L+LQ+ L+D+ VLSK+R+  GGN+K+S VGG+  ++ V  FFE  + I ILEGYGL+ETSP+++++ PE + RRLGS G+ +    + ++  G  +P GEEGEV  +GP V +GY +  E ++    +L+G+++FRTGDLGR+ +G +L ITGR+KE YKLENGKYV    +E A+  S YI+Q ++ G + PFNVAL+ P+W  +  WA S   A  +   S EEL++   V+  +   I L+  D  KKYEMP+ + LL E FT EN M T K+S KRH VIK Y   L  +Y
Sbjct:  120 VDRCRAALAGLGITHGDTVGIISNNREEWAVCAYATYSLGAVFVPMYEEQRPEDWRYILEDSGAKCLFVATKGIYRETFHF------------------------AGVQGNVQNV-----FCFDQPAGQPGSFKDLIDTNAEEKVPAYIPDPSELATLIYTSGTTGKPKGVMLSHGNIISNIVGI-RAILPDDLVSCHDRSLSFLPWAHCYGQTAELHALMAHGASMGISRDVTALLDQLAEVKPTLLYAVPQLFKRVCDAIHAKVAESSPAKQYLFKRSLQMAHRRRKHIEAGEPVGAALELQHRLLDQAVLSKIRDRFGGNLKVSFVGGAATNMEVLNFFE-NIDIKILEGYGLTETSPMVTVNTPEQKFRRLGSTGRCLADVNVKIMMGGEEMPAGEEGEVCVSGPNVMQGYNNLPEASSEVLFDLDGQRYFRTGDLGRMDDGTYLRITGRIKEQYKLENGKYVVPGPIEAAMTSSTYITQALVFGDNRPFNVALVFPDWNLVRSWAESKGRA--NDGTSLEELASMDVVKNLIAGEIALSL-DGFKKYEMPRTFELLEEGFTKENNMQTQKLSTKRHVVIKHYHDTLMHMY 680          

BLAST of NO25G00730 vs. NCBI_GenBank
Match: WP_012828673.1 (long-chain fatty acid--CoA ligase [Haliangium ochraceum] >ACY16074.1 AMP-dependent synthetase and ligase [Haliangium ochraceum DSM 14365])

HSP 1 Score: 399.8 bits (1026), Expect = 1.500e-107
Identity = 230/596 (38.59%), Postives = 353/596 (59.23%), Query Frame = 0

Query:    1 MVDKAKYLLQQAGIGPGDKVAVISRNRVEWAVGCMASLSLGATWVPMYEEQRMNECEYILKDSGAKLLLVGSEETYDKTRHFLASNXXXXXXXXXXXXXXXXXXXQQGFQGPVPSLQDIWTFDIDVPARMAMVSP-SMLASSP-SSPHIHADDVAVLIYTSGTTGTPKGVELTHANLCSNIQGMWEVVKPEDMP---DALSLAFLPWAHSIGLNCELFNMMSRGARVALAQDIKTIASDIKEVKPNILFAVPTLYKKVYDKIQDKINSSGRWSRWLVDTAVAVAEKRLQVLREGGVLSLVLQLQYNLVDRIVLSKLRETMGGNVKLSPVGGSKMSLPVTRFFEAALGIAILEGYGLSETSPLLSISRPEVQTRRLGSVGQAVKGAELAVV-RDGRRVPEGEEGEVWAAGPMVFKGYLSKEEETAAAFGELEGRKWFRTGDLGRLS-EGFLTITGRLKELYKLENGKYVCAPVVEKALLESEYISQVVLHGADLPFNVALIVPNWEKLTLWALSSKLAGLSPTPSKEELSAHPEVQAFMRQTIVLACKDKVKKYEMPQEWLLLTEAFTVENEMLTPKMSIKRHAVIKAYLPALTALY 590
            +V++ +  L Q G+  GD+VAV+S NRVEWAVG  A+ SLG  +VPMYE Q   E  YI++DS +K+ L  +E  Y +                             G  G V SL+    FD    A  + +   ++ A +P SS H   DD+A L+YTSGTTG PKGV L+H N+CSN++     V P+  P   + ++ +FLPWAH+ G   EL  ++S GA + LA+ ++T+  +  EV+P +L AVP ++ ++YD ++ ++    +  + L + A++V++KR + +R+ G  S +L +Q+ L +R+V +K++E  GG ++    GG+ +++ V  F +  LGI + EGYGL+ETSP+ +++RP    RR+G+VGQA+    + +    G+ +P   +GEV  +GP + +GY    E TA    E++G++ FRTGD+GRLS +GFL+ITGR KE YKLENGKYV   ++E  L  S +I+Q  ++G + PFNV LIVP++E +  WA S +  G+S   S E L A   V A + + +  A   + K +E P+ W LL E F+ EN ++TPKMS++R AV   Y   L  LY
Sbjct:   44 LVNRFRAALAQLGVEAGDRVAVVSNNRVEWAVGAYATYSLGGHYVPMYEAQSPAERRYIIEDSNSKVALAATEAIYQEL---------------------------AGLIGEVDSLEHAICFDASESAPHSYLHHLAVGAENPVSSVHPSPDDLAGLLYTSGTTGKPKGVMLSHNNICSNLR-----VMPDLFPLSHEDVTCSFLPWAHAFGQTAELHGIISAGASMGLAESVQTLMENFLEVRPTVLLAVPRIFHRIYDGVKKRMADEPKLRQKLFEHALSVSQKRTE-MRDHGGSSTLLDIQHKLFERVVFAKIKERFGGRLRFVMSGGAALAVEVAEFID-NLGITVFEGYGLTETSPVATVNRP--GARRIGTVGQAIPDVSIYICDEQGKLLPPETDGEVLISGPNIMQGYHGLPEATAEVMVEVDGKQAFRTGDMGRLSQDGFLSITGRFKEQYKLENGKYVVPTLLEDQLKLSGFITQAFIYGDNKPFNVCLIVPDFEAIQKWAKSDEGGGVSGDLSPEALCADARVHAKIGEELA-AYGAEFKHFEQPKRWKLLVEDFSPENGLMTPKMSVRRRAVTARYQDELDKLY 602          

BLAST of NO25G00730 vs. NCBI_GenBank
Match: WP_011552606.1 (long-chain fatty acid--CoA ligase [Myxococcus xanthus] >ABF88081.1 putative long-chain-fatty-acid--CoA ligase [Myxococcus xanthus DK 1622] >SDW94184.1 long-chain acyl-CoA synthetase [Myxococcus xanthus])

HSP 1 Score: 388.3 bits (996), Expect = 4.600e-104
Identity = 241/602 (40.03%), Postives = 342/602 (56.81%), Query Frame = 0

Query:    1 MVDKAKYLLQQAGIGPGDKVAVISRNRVEWAVGCMASLSLGATWVPMYEEQRMNECEYILKDSGAKLLLVGSEETYDKTRHFLASNXXXXXXXXXXXXXXXXXXXQQGFQGPVPSLQDIWTF-----DIDVPA---RMAMVSPSMLASSPSSPHIHADDVAVLIYTSGTTGTPKGVELTHANLCSNIQGMWEVVKPEDMPDALSLAFLPWAHSIGLNCELFNMMSRGARVALAQDIKTIASDIKEVKPNILFAVPTLYKKVYDKIQDKINSSGRWSRWLVDTAVAVAEKRLQVLREGGVLSLVLQLQYNLVDRIVLSKLRETMGGNVKLSPVGGSKMSLPVTRFFEAALGIAILEGYGLSETSPLLSISRPEVQTRRLGSVGQAVKGAELAVVRDGRRVPEGEEGEVWAAGPMVFKGYLSKEEETAAAFGELEGRKWFRTGDLGRLS-EGFLTITGRLKELYKLENGKYVCAPVVEKALLESEYISQVVLHGADLPFNVALIVPNWEKLTLWALSSKLAGLSPTPSKEELSAHPEVQAFMRQTIVLACKDKVKKYEMPQEWLLLTEAFTVENEMLTPKMSIKRHAVIKAYLPALTALYEQAA 594
            MVD  +  L Q G+G GD+VAVIS NR+EWAVG  A+ +LG  +VPMYE Q++ E ++IL DSGAK++   +++   +                            Q  +  +P L+ I  F     D D  A   R    +P+ L S   +      D+A LIYTSGTTG PKGV L+HAN+  N+  M EV  P    D  SLAFLPWAH  G   EL  ++S GA +A+A+ ++ I  ++ EVKP +LF+VP ++ ++YD +Q ++      +R++    +AVA +R + L E G  S +L LQ+   D++V SK+R   GG +K +  GGS +S  V  F +  LGI + EGYGL+ETSP+ + + P    R++GSVG+A+ G  + +  D     E  +GE+   G  V  GY +K EE    F    G   FRTGD+G L  +G+L ITGR+KE YKLENGKYV    +E++L  S YI+  ++HG + P+NVA+IV + + L  WA    L     T S  EL   PEV    R+ +    +D VK YE PQ +LL++E FTV N+MLTPKMS+KR  V+  Y  A+ ALY + +
Sbjct:   45 MVDDLRGGLAQLGVGAGDRVAVISNNRLEWAVGAYATYTLGGAYVPMYESQQVKELQFILNDSGAKVVFCATDDIAQRI---------------------------QSVRAELPQLEHIIRFSGTTSDTDSFATLLRRGAETPTPLVSPKPA------DLAGLIYTSGTTGQPKGVMLSHANIARNVSAMHEVF-PMGTEDR-SLAFLPWAHVFGQTVELHALLSMGASMAIAEAVEKIIDNLSEVKPTLLFSVPRIFNRIYDGLQKRMAGEKAVTRFMFHRGLAVAAQR-RALAEAGKSSGLLDLQHAFFDKVVFSKVRARFGGRLKYAFSGGSAISKEVAEFID-NLGITVYEGYGLTETSPIATANFP--NNRKIGSVGKALPGIRVEI--DTAATGEATQGEIVVHGHNVMMGYYNKPEENEKVF---TGNGGFRTGDMGYLDPDGYLYITGRIKEQYKLENGKYVVPSPIEQSLALSTYIANALVHGMNKPYNVAIIVVDVDTLKKWATEKGL----DTTSMPELLKRPEVLQLYREQLNEFTRD-VKGYERPQRFLLISEDFTVANDMLTPKMSVKRRNVVARYNDAIEALYREGS 597          

BLAST of NO25G00730 vs. NCBI_GenBank
Match: WP_013939111.1 (long-chain fatty acid--CoA ligase [Myxococcus fulvus] >AEI65972.1 putative long-chain-fatty-acid--CoA ligase [Myxococcus fulvus HW-1])

HSP 1 Score: 387.9 bits (995), Expect = 6.000e-104
Identity = 241/603 (39.97%), Postives = 340/603 (56.38%), Query Frame = 0

Query:    1 MVDKAKYLLQQAGIGPGDKVAVISRNRVEWAVGCMASLSLGATWVPMYEEQRMNECEYILKDSGAKLLLVGSEETYDKTRHFLASNXXXXXXXXXXXXXXXXXXXQQGFQGPVPSLQDIWTF-----DIDVPA----RMAMVSPSMLASSPSSPHIHADDVAVLIYTSGTTGTPKGVELTHANLCSNIQGMWEVVKPEDMPDALSLAFLPWAHSIGLNCELFNMMSRGARVALAQDIKTIASDIKEVKPNILFAVPTLYKKVYDKIQDKINSSGRWSRWLVDTAVAVAEKRLQVLREGGVLSLVLQLQYNLVDRIVLSKLRETMGGNVKLSPVGGSKMSLPVTRFFEAALGIAILEGYGLSETSPLLSISRPEVQTRRLGSVGQAVKGAELAVVRDGRRVPEGEEGEVWAAGPMVFKGYLSKEEETAAAFGELEGRKWFRTGDLGRLS-EGFLTITGRLKELYKLENGKYVCAPVVEKALLESEYISQVVLHGADLPFNVALIVPNWEKLTLWALSSKLAGLSPTPSKEELSAHPEVQAFMRQTIVLACKDKVKKYEMPQEWLLLTEAFTVENEMLTPKMSIKRHAVIKAYLPALTALYEQAA 594
            MVD  +  L Q G+G GD+VAVIS NR+EWAVG  A+ SLG  +VPMYE Q+  E ++I+ DSG+K++   ++E   +                            Q  +  +P L+ I  F     D D  A    R A     M++  P+       D+A LIYTSGTTG PKGV L+HAN+  N+  M EV       D  SLAFLPWAH  G   EL  + S GA +A+A+ ++ I  ++ EVKP +LF+VP ++ ++YD +Q ++      +R++    +AVA ++ + L E G  S +L LQ+   D++V SK+R   GG +K +  GGS +S  V  F +  LGI + EGYGL+ETSP+ + + PE   R++GSVG+A+ G  + +  D     E ++GE+   G  V  GY +K EE    F    G   FRTGD+G L  +G+L ITGR+KE YKLENGKYV    +E++L  S YI+  ++HG + P+NVA+IV + + L  WA    L     T S  EL   PEV    R+ +    +D VK YE PQ +LL +E FTV N+MLTPKMS+KR  V+  Y  A+ ALY + A
Sbjct:   45 MVDDLRGGLAQLGVGAGDRVAVISNNRLEWAVGAYATYSLGGAYVPMYESQQAKELQFIINDSGSKVVFCATDEIAQRI---------------------------QSVRAELPHLEHIIRFSGTTSDTDSFATLLRRGAETPTPMVSPKPT-------DLAGLIYTSGTTGQPKGVMLSHANIARNVSAMHEVFPMG--TDDRSLAFLPWAHVFGQTVELHALFSMGASMAIAEAVEKIIDNLSEVKPTLLFSVPRIFNRIYDGLQKRMAGEKPVTRFMFRRGLAVAAEK-RALAEQGKSSGLLDLQHAFFDKVVFSKVRARFGGRLKYAFSGGSAISKEVAEFID-NLGITVYEGYGLTETSPIATANFPE--NRKIGSVGKALPGVRIEI--DTAATGEAKQGEIVVHGHNVMMGYYNKPEENEKVF---TGNGGFRTGDMGYLDPDGYLYITGRIKEQYKLENGKYVVPSPIEQSLALSTYIANALVHGMNKPYNVAIIVVDVDALKKWATEKGL----DTSSMPELLKRPEVLQLYREQVNEFTRD-VKGYERPQRFLLTSEDFTVANDMLTPKMSVKRRNVVARYNDAIEALYREGA 597          

BLAST of NO25G00730 vs. NCBI_GenBank
Match: WP_002639036.1 (long-chain fatty acid--CoA ligase [Myxococcus hansupus] >AKQ67593.1 Long-chain-fatty-acid--CoA ligase [Myxococcus hansupus])

HSP 1 Score: 387.5 bits (994), Expect = 7.800e-104
Identity = 239/604 (39.57%), Postives = 344/604 (56.95%), Query Frame = 0

Query:    1 MVDKAKYLLQQAGIGPGDKVAVISRNRVEWAVGCMASLSLGATWVPMYEEQRMNECEYILKDSGAKLLLVGSEETYDKTRHFLASNXXXXXXXXXXXXXXXXXXXQQGFQGPVPSLQDIWTF-----DIDVPA---RMAMVSPSMLASSPSSPHIHADDVAVLIYTSGTTGTPKGVELTHANLCSNIQGMWEV--VKPEDMPDALSLAFLPWAHSIGLNCELFNMMSRGARVALAQDIKTIASDIKEVKPNILFAVPTLYKKVYDKIQDKINSSGRWSRWLVDTAVAVAEKRLQVLREGGVLSLVLQLQYNLVDRIVLSKLRETMGGNVKLSPVGGSKMSLPVTRFFEAALGIAILEGYGLSETSPLLSISRPEVQTRRLGSVGQAVKGAELAVVRDGRRVPEGEEGEVWAAGPMVFKGYLSKEEETAAAFGELEGRKWFRTGDLGRL-SEGFLTITGRLKELYKLENGKYVCAPVVEKALLESEYISQVVLHGADLPFNVALIVPNWEKLTLWALSSKLAGLSPTPSKEELSAHPEVQAFMRQTIVLACKDKVKKYEMPQEWLLLTEAFTVENEMLTPKMSIKRHAVIKAYLPALTALYEQAA 594
            MVD  +  L Q G+G GD+VAVIS NR+EWAVG  A+ +LG  +VPMYE Q++ E ++I+ DSGAK++   ++E   +                            Q  +  +P+++ +  F     D D  A   R    +P+ L S   +      D+A LIYTSGTTG PKGV L+H N+  N+  M EV  + PED     SLAFLPWAH  G   EL  ++S GA +A+A+ ++ I  ++ EVKP +LF+VP ++ ++YD +Q ++      +R++    +AVA +R + L E G  S +L LQ+   D++V SK+R   GG +K +  GGS +S  V  F +  LGI + EGYGL+ETSP+ + + PE   R++GSVG+A+ G  + +  D     E  +GE+   G  V  GY +K EE    F    G   FRTGD+G L  +G+L ITGR+KE YKLENGKYV    +E++L  S +I+  ++HG + P+NV +IV + + L  WA    L     T S  EL   PEVQ   R+ +    +D VK YE PQ +LL +E FTV N+MLTPKMS+KR  V+  Y  A+ ALY + +
Sbjct:   45 MVDDLRGGLAQLGVGAGDRVAVISNNRLEWAVGAYATYTLGGAYVPMYESQQVKELQFIINDSGAKVVFCATDEIAQRL---------------------------QAVRAELPNVEHLIRFSGTTSDADSFATLLRRGAETPTPLVSPKPT------DLAGLIYTSGTTGQPKGVMLSHQNIARNVSAMHEVFPMTPEDR----SLAFLPWAHVFGQTVELHALLSMGASMAVAEAVEKIIDNLSEVKPTLLFSVPRIFNRIYDGLQKRMAGEKAVTRFMFHRGLAVAAQR-RALAEQGKSSGLLDLQHAFFDKVVFSKVRARFGGRLKYAFSGGSAISKEVAEFID-NLGITVYEGYGLTETSPIATANFPE--NRKIGSVGKALPGVRIEI--DTAATGEATQGEIVVHGHNVMMGYYNKPEENEKVF---TGNGGFRTGDMGLLDKDGYLYITGRIKEQYKLENGKYVVPSPIEQSLTLSTFIANALVHGMNKPYNVVVIVVDVDTLKKWATEKGL----DTSSLPELLKRPEVQQLYREQVNEFTRD-VKGYERPQRFLLTSEDFTVANDMLTPKMSVKRRNVVARYNDAIEALYREGS 597          

BLAST of NO25G00730 vs. NCBI_GenBank
Match: WP_095958260.1 (long-chain fatty acid--CoA ligase [Myxococcus macrosporus] >ATB46893.1 long-chain-fatty-acid--CoA ligase [Myxococcus macrosporus DSM 14697])

HSP 1 Score: 386.3 bits (991), Expect = 1.700e-103
Identity = 240/603 (39.80%), Postives = 340/603 (56.38%), Query Frame = 0

Query:    1 MVDKAKYLLQQAGIGPGDKVAVISRNRVEWAVGCMASLSLGATWVPMYEEQRMNECEYILKDSGAKLLLVGSEETYDKTRHFLASNXXXXXXXXXXXXXXXXXXXQQGFQGPVPSLQDIWTF-----DIDVPA----RMAMVSPSMLASSPSSPHIHADDVAVLIYTSGTTGTPKGVELTHANLCSNIQGMWEVVKPEDMPDALSLAFLPWAHSIGLNCELFNMMSRGARVALAQDIKTIASDIKEVKPNILFAVPTLYKKVYDKIQDKINSSGRWSRWLVDTAVAVAEKRLQVLREGGVLSLVLQLQYNLVDRIVLSKLRETMGGNVKLSPVGGSKMSLPVTRFFEAALGIAILEGYGLSETSPLLSISRPEVQTRRLGSVGQAVKGAELAVVRDGRRVPEGEEGEVWAAGPMVFKGYLSKEEETAAAFGELEGRKWFRTGDLGRLS-EGFLTITGRLKELYKLENGKYVCAPVVEKALLESEYISQVVLHGADLPFNVALIVPNWEKLTLWALSSKLAGLSPTPSKEELSAHPEVQAFMRQTIVLACKDKVKKYEMPQEWLLLTEAFTVENEMLTPKMSIKRHAVIKAYLPALTALYEQAA 594
            MVD  +  L Q G+G GD+VAVIS NR+EWAVG  A+ SLG  +VPMYE Q+  E ++I+ DSG+K++   ++E   +                            Q  +  +P L+ I  F     D D  A    R A     M++  P+       D+A LIYTSGTTG PKGV L+HAN+  N+  M EV       D  SLAFLPWAH  G   EL  + S GA +A+A+ ++ I  ++ EVKP +LF+VP ++ ++YD +Q ++      +R++    +AVA ++ + L E G  S +L LQ+   D++V SK+R   GG +K +  GGS +S  V  F +  LGI + EGYGL+ETSP+ + + PE   R++GSVG+A+ G  + +  D     E ++GE+   G  V  GY +K EE    F    G   FRTGD+G L  +G+L ITGR+KE YKLENGKYV    +E++L  S +I+  ++HG + P+NVA+IV + + L  WA    L     T S  EL   PEV    R+ +    +D VK YE PQ +LL +E FTV N+MLTPKMS+KR  V+  Y  A+ ALY + A
Sbjct:   45 MVDDLRGGLAQLGVGAGDRVAVISNNRLEWAVGAYATYSLGGAYVPMYESQQAKELQFIINDSGSKVVFCATDEIAQRI---------------------------QSVRAELPHLEHIIRFSGTTSDTDSFATLLRRGAETPTPMVSPKPT-------DLAGLIYTSGTTGQPKGVMLSHANIARNVSAMHEVFPMG--TDDRSLAFLPWAHVFGQTVELHALFSMGASMAIAEAVEKIIDNLSEVKPTLLFSVPRIFNRIYDGLQKRMAGEKPVTRFMFRRGLAVAAEK-RALAEQGKSSGLLDLQHAFFDKVVFSKVRARFGGRLKYAFSGGSAISKEVAEFID-NLGITVYEGYGLTETSPIATANFPE--NRKIGSVGKALPGVRIEI--DTAATGEAKQGEIVVHGHNVMMGYYNKPEENEKVF---TGNGGFRTGDMGYLDPDGYLYITGRIKEQYKLENGKYVVPSPIEQSLALSTFIANALVHGMNKPYNVAIIVVDVDALKKWATEKGL----DTSSMPELLKRPEVLQLYREQVNEFTRD-VKGYERPQRFLLTSEDFTVANDMLTPKMSVKRRNVVARYNDAIEALYREGA 597          

BLAST of NO25G00730 vs. NCBI_GenBank
Match: WP_090489343.1 (long-chain fatty acid--CoA ligase [Myxococcus virescens] >SDD93266.1 long-chain acyl-CoA synthetase [Myxococcus virescens])

HSP 1 Score: 386.0 bits (990), Expect = 2.300e-103
Identity = 239/602 (39.70%), Postives = 342/602 (56.81%), Query Frame = 0

Query:    1 MVDKAKYLLQQAGIGPGDKVAVISRNRVEWAVGCMASLSLGATWVPMYEEQRMNECEYILKDSGAKLLLVGSEETYDKTRHFLASNXXXXXXXXXXXXXXXXXXXQQGFQGPVPSLQDIWTF-----DIDVPA---RMAMVSPSMLASSPSSPHIHADDVAVLIYTSGTTGTPKGVELTHANLCSNIQGMWEVVKPEDMPDALSLAFLPWAHSIGLNCELFNMMSRGARVALAQDIKTIASDIKEVKPNILFAVPTLYKKVYDKIQDKINSSGRWSRWLVDTAVAVAEKRLQVLREGGVLSLVLQLQYNLVDRIVLSKLRETMGGNVKLSPVGGSKMSLPVTRFFEAALGIAILEGYGLSETSPLLSISRPEVQTRRLGSVGQAVKGAELAVVRDGRRVPEGEEGEVWAAGPMVFKGYLSKEEETAAAFGELEGRKWFRTGDLGRLS-EGFLTITGRLKELYKLENGKYVCAPVVEKALLESEYISQVVLHGADLPFNVALIVPNWEKLTLWALSSKLAGLSPTPSKEELSAHPEVQAFMRQTIVLACKDKVKKYEMPQEWLLLTEAFTVENEMLTPKMSIKRHAVIKAYLPALTALYEQAA 594
            MVD  +  L Q G+G GD+VAVIS NR+EWAVG  A+ +LG  +VPMYE Q++ E ++IL DSG+K++   +++   +                            Q  +  +P L+ I  F     D D  A   R    +P+ L S   +      D+A LIYTSGTTG PKGV L+H+N+  N+  M EV  P    D  SLAFLPWAH  G   EL  ++S GA +A+A+ ++ I  ++ EVKP +LF+VP ++ ++YD +Q ++      +R++    +AVA +R + L E G  S +L LQ+   D++V SK+R   GG +K +  GGS +S  V  F +  LGI + EGYGL+ETSP+ + + P    R++GSVG+A+ G  + +  D     E  +GE+   G  V  GY +K EE    F    G   FRTGD+G L  +G+L ITGR+KE YKLENGKYV    +E++L  S YI+  ++HG + P+NVA+IV + + L  WA    L     T S  EL   PEV    R+ +    +D VK YE PQ +LL++E FTV N+MLTPKMS+KR  V+  Y  A+ ALY + +
Sbjct:   45 MVDDLRGGLAQLGVGAGDRVAVISNNRLEWAVGAYATYTLGGAYVPMYESQQVKELQFILNDSGSKVVFCATDDIAQRI---------------------------QAVRAELPHLEHIIRFSGTTSDTDSFATLLRRGAETPTPLVSPKPT------DLAGLIYTSGTTGQPKGVMLSHSNIARNVSAMHEVF-PMGTEDR-SLAFLPWAHVFGQTVELHALLSMGASMAIAEAVEKIIDNLSEVKPTLLFSVPRIFNRIYDGLQKRMAGEKAVTRFMFHRGLAVAAQR-RALAEAGKSSGLLDLQHAFFDKVVFSKVRARFGGRLKYAFSGGSAISKEVAEFID-NLGITVYEGYGLTETSPIATANFP--NNRKIGSVGKALPGVRVEI--DTAATGEATQGEIVVHGHNVMMGYYNKPEENEKVF---TGNGGFRTGDMGYLDPDGYLYITGRIKEQYKLENGKYVVPSPIEQSLALSTYIANALVHGMNKPYNVAIIVVDVDTLKKWAAEKGL----DTTSMPELLKRPEVLQLYREQLNEFTRD-VKGYERPQRFLLISEDFTVANDMLTPKMSVKRRNVVARYNDAIEALYREGS 597          

BLAST of NO25G00730 vs. NCBI_GenBank
Match: XP_013756570.1 (hypothetical protein AMSG_12039 [Thecamonas trahens ATCC 50062] >KNC51096.1 hypothetical protein AMSG_12039 [Thecamonas trahens ATCC 50062])

HSP 1 Score: 373.2 bits (957), Expect = 1.500e-99
Identity = 236/634 (37.22%), Postives = 344/634 (54.26%), Query Frame = 0

Query:    9 LQQAGIGPGDKVAVISRNRVEWAVGCMASLSLGATWVPMYEEQRMNECEYILKDSGAKLLLVGSEETYDKTRHFLASNXXXXXXXXXXXXXXXXXXXQQGFQGPVPSLQDIWTFD----IDVPARMAMVS--------PSMLASSPSSPHIHADDVAVLIYTSGTTGTPKGVELTHANLCSNIQG------------------------MWEVVKPEDMPDA--LSLAFLPWAHSIGLNCELFNMMSRGARVALAQDIKTIASDIKEVKPNILFAVPTLYKKVYDKIQDKINSSGRWSRWLVDTAVAVAEKRLQVLREGGVLSLVLQLQYNLVDRIVLSKLRETMGGNVKLSPVGGSKMSLPVTRFFEAALGIAILEGYGLSETSPLLSISRPEVQTRRLGSVGQAVKGAELAVVR--DGRRV---PEGEEGEVWAAGPMVFKGYLSKEEETA------AAFGELEGRKWFRTGDLGRL-SEGFLTITGRLKELYKLENGKYVCAPVVEKALLESEYISQVVLHGADLPFNVALIVPNWEKLTLWALSSKLAGLSPTPSKEELSAHPEVQAFMRQTIVLACKDKVKKYEMPQEWLLLTEAFTVENEMLTPKMSIKRHAVIKAYLPALTALYEQA 593
            L   G   G +VA++S N VEWAV   A+LSLGA +VPMYE Q  +  +YI++DSGA++LLV     ++K  H +                       Q   G    L    TF     ++  +R+  +S         S+    P SP   ADD+A +IYTSGTTGTPKGV L+H N+ +N++                         +W +     + D   +SL+FLPWAH+ G  CEL +++  G  + L + + TI  D K+V P +LF+VP L+ ++YD I +K+ S     R L +  +A+  ++L     G  LS++  L   ++D+ +L+K+RET GG ++++ VGG+ M   + +  + ALGI + EGYGL+ETSP+ +++ P    R+ G+VG  + G ++ + +  DG  V   P G +GEV  AG  V   Y +K E TA      A  G  +  + FRTGDLGRL ++GFLTITGR+KE YKLENGK+V    VE  L  S +ISQ  +HGA++P NVALIVP+W  L  WA      GL    S   L     V+A +   I   C   +K YE P  WL L E F+ +N+MLTPK+S+KRH +++ Y   + AL+ +A
Sbjct: 1650 LAALGTQKGSRVAIVSNNSVEWAVAYYATLSLGAVFVPMYEAQMPSTWKYIIEDSGAEVLLVSKPAVFNKALHLVDDIDPLHAIIPLGGLDPESALRAQVSFG----LDAETTFGSGAALEAGSRVPFLSYRDALSYGASIRDDEPPSPPA-ADDLATIIYTSGTTGTPKGVALSHRNIVANLKAIQSGNAAXXXXXXXXXXXXGRPTELWGLSPSGLLYDGSDVSLSFLPWAHAYGQTCELNSLLVEGGAIGLVEHVTTILEDFKKVSPTVLFSVPALFNRIYDGINNKLASKPPIVRSLFEKTIALRTRQL-AGASGPPLSILDTLILKVLDKAILTKIRETFGGRLRVAFVGGAAMDPHIMQLLD-ALGITVFEGYGLTETSPIATLNTP--GARKYGTVGPPIAGVDIVIAKSFDGDLVDVAPSGTDGEVCIAGDNVMVEYYNKPEATAEAVVENARVGPGKSLRLFRTGDLGRLDADGFLTITGRIKEQYKLENGKFVVPAPVEDKLNLSRFISQSFIHGANMPSNVALIVPDWAALRAWAAEH---GLDAGASTTSLLDSSRVRALLDAEIAAGCA-ALKSYERPAAWLYLREPFSTDNDMLTPKLSLKRHNILQTYASQVDALHAEA 2270          

BLAST of NO25G00730 vs. NCBI_GenBank
Match: WP_095977778.1 (long-chain fatty acid--CoA ligase [Melittangium boletus] >ATB29173.1 long-chain-fatty-acid--CoA ligase [Melittangium boletus DSM 14713])

HSP 1 Score: 372.9 bits (956), Expect = 2.000e-99
Identity = 225/595 (37.82%), Postives = 335/595 (56.30%), Query Frame = 0

Query:    2 VDKAKYLLQQAGIGPGDKVAVISRNRVEWAVGCMASLSLGATWVPMYEEQRMNECEYILKDSGAKLLLVGSEETYDKTRHFLASNXXXXXXXXXXXXXXXXXXXQQGFQGPVPSLQDIWTF---DIDVPARMAMVSPSMLASSPSSPHIHADDVAVLIYTSGTTGTPKGVELTHANLCSNIQGMWEVVKPEDMPDALSLAFLPWAHSIGLNCELFNMMSRGARVALAQDIKTIASDIKEVKPNILFAVPTLYKKVYDKIQDKINSSGRWSRWLVDTAVAVAEKRLQVLREGGVLSLVLQLQYNLVDRIVLSKLRETMGGNVKLSPVGGSKMSLPVTRFFEAALGIAILEGYGLSETSPLLSISRPEVQTRRLGSVGQAVKGAELAVVRDGRRVPEGEEGEVWAAGPMVFKGYLSKEEETAAAFGELEGRKWFRTGDLGRLSE-GFLTITGRLKELYKLENGKYVCAPVVEKALLESEYISQVVLHGADLPFNVALIVPNWEKLTLWALSSKLAGLSPTPSKEELSAHPEVQAFMRQTIVLACKDKVKKYEMPQEWLLLTEAFTVENEMLTPKMSIKRHAVIKAYLPALTALYEQA 593
            VD  +  L + G+  GD+VAVIS NR EWAVG  AS +LGA +VPMYE+Q+  E +YIL D GAK++ V ++    K                               +  +P+L+ +  F   + D  +   ++S    +  P++      D++ LIYTSGTTG PKGV+L+H N+ SN+  M E+    D  D  SLAFLPWAH  G + EL  + S GA + +A+  + I  ++ EVKP+++F+VP ++ ++YD +Q ++ +     R L    + VA++R + L E    S +L LQ+   D++V SK+R   GG +K +  GG+ +S  V  F +  LGI + EGYGL+ETSP+ + + P    R++GSVG+ + G  + +  D     + ++GE+ A G  + +GY +  EE   AF    G   FRTGD+G + E G+L ITGR+KE YKLENGKYV    +E++L  S +I   +LHG + PFN A+IVP+   LT WA    L+   P      L   PEVQ   R+ I    KD VK YE P+ +LL++E FT  N+MLTP + +KR +V+K Y   +  +Y +A
Sbjct:   46 VDDLRGGLAKLGVTAGDRVAVISNNRHEWAVGAYASYTLGAAYVPMYEQQQEKEWQYILNDCGAKVVFVATDAIAKKI---------------------------SALKSSLPALEHVIRFSGGEQDADSLANLLSQGSASPQPAASP-KPSDLSGLIYTSGTTGNPKGVKLSHGNIASNVAAMHEIFPMSD--DDRSLAFLPWAHVFGQSVELHGLYSMGASMGIAESTEKIIENLAEVKPSLIFSVPRIFNRIYDGLQKRMAAESPLKRLLFTRGIEVAKQR-KALAEQKRTSALLDLQHAFFDKVVFSKVRARFGGRMKYAFSGGAAISREVAEFID-NLGITVYEGYGLTETSPIATANYP--GNRKIGSVGKPLPGTRVEI--DRTETGDPKQGEIVAFGHNIMQGYYNLPEEDEKAFTPEHG---FRTGDMGYVDEDGYLWITGRIKEQYKLENGKYVSPAPIEQSLQLSSFIVNAMLHGQNKPFNTAIIVPDMGSLTKWAEEKGLSTAMPA-----LLTLPEVQQLYREQIAEYTKD-VKGYEKPKHFLLVSEDFTTANDMLTPSLKLKRRSVLKKYGEEVENIYREA 595