NO25G00350, NO25G00350 (gene) Nannochloropsis oceanica

Overview
NameNO25G00350
Unique NameNO25G00350
Typegene
OrganismNannochloropsis oceanica (N. oceanica IMET1)
Sequence length1282
Alignment locationchr25:105624..106905 -

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Properties
Property NameValue
DescriptionRab family gtpase
Mutants
Expression

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
chr25genomechr25:105624..106905 -
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
PRJNA7699582024-08-13
GSE1786722023-12-15
PRJNA9336932023-10-26
PRJNA9434492023-07-19
GSE1499042020-10-10
NoIMET1_WT_HS2020-09-29
GSE1396152020-03-11
PRJNA2413822020-03-11
BLASTP analysis of N. oceanica IMET1 genes2019-07-11
InterPro analysis for N. oceanica IMET1 genes2019-07-11
GO annotation for IMET1v2 genes2019-07-10
PRJNA1821802019-04-25
Gene prediction for N. oceanica IMET12017-10-24
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005525GTP binding
GO:0003924GTPase activity
GO:0005525GTP binding
GO:0003924GTPase activity
Vocabulary: INTERPRO
TermDefinition
IPR027417P-loop_NTPase
IPR001806Small_GTPase
Homology
BLAST of NO25G00350 vs. NCBI_GenBank
Match: EWM21552.1 (rab family gtpase [Nannochloropsis gaditana])

HSP 1 Score: 234.6 bits (597), Expect = 3.200e-58
Identity = 122/199 (61.31%), Postives = 143/199 (71.86%), Query Frame = 0
Query:   36 MTIGVEYGLKPAQVKGRDIQIEIWDTAGQECYMALTRSYYRNADGCLVCFDLTNRSSFDSVSKWVEHARSNSGNPYLVLTLVGNKADCQGRRQVARAEAEALAAHHGMEYREITAKDNEAVQGLFHTTAERIFKAVESGPDGEAGAVGSDIVIDGAGRVXXXXXXXXXXXXXXGNIVLAPGKASVNPSSAAEGG---CC 232
            MT+GVEYGLK A V+ ++IQIEIWDTAGQE YM+LT SYYRNADGCL+ FDLT R SF+ VSKW EHAR  S NP+LV TLVGNKAD    RQVAR EAEA+AAH GMEYRE+TAKD+ AVQGLF TTAERIF+A E+G   + G  GSDIVIDGAG++              G   L+PG+++ +   A   G   CC
Sbjct:    1 MTVGVEYGLKSATVRDQNIQIEIWDTAGQEAYMSLTNSYYRNADGCLIVFDLTQRESFEKVSKWAEHARQYSNNPHLVFTLVGNKADQAALRQVAREEAEAVAAHQGMEYREVTAKDHTAVQGLFQTTAERIFQAFETGAGLKGGMGGSDIVIDGAGKIRLRSFNSSLSSSAFG---LSPGRSARSQEMAGNSGKGRCC 196          
BLAST of NO25G00350 vs. NCBI_GenBank
Match: CBN76817.1 (Rab2B, RAB family GTPase [Ectocarpus siliculosus])

HSP 1 Score: 188.0 bits (476), Expect = 3.400e-44
Identity = 85/182 (46.70%), Postives = 128/182 (70.33%), Query Frame = 0
Query:    1 MPSLVFKFIVVGNTDVGKSSLLLQFVEQRFNEKHAMTIGVEYGLKPAQVKGRDIQIEIWDTAGQECYMALTRSYYRNADGCLVCFDLTNRSSFDSVSKWVEHARSNSGNPYLVLTLVGNKADCQGRRQVARAEAEALAAHHGMEYREITAKDNEAVQGLFHTTAERIFKAVESGPDGEAGAV 183
            MP+L+FK+I++GNT VGKS++LLQF E++F + H MTIGVE+G K   +    +++EIWDTAGQE ++++TRSYYR  DGCL+ FD+T+R SF+++ +W++ AR NS NP L + L+ NKAD   +R V   E +A A  HG+ + E++A++   V+  F  TAERIF  +E+ P  +   +
Sbjct:    1 MPTLLFKYIIIGNTAVGKSAILLQFTERQFRDAHDMTIGVEFGAKDVSIGDAQVKLEIWDTAGQESFLSITRSYYRGTDGCLLVFDITDRKSFEALPRWLDEARMNSANPDLSVMLIANKADLAEKRCVTTEEGQAFADKHGLLFMEMSARNAAQVERAFIGTAERIFSDLEAHPRNKPSGI 182          
BLAST of NO25G00350 vs. NCBI_GenBank
Match: XP_009034590.1 (hypothetical protein AURANDRAFT_22559 [Aureococcus anophagefferens] >EGB11031.1 hypothetical protein AURANDRAFT_22559 [Aureococcus anophagefferens])

HSP 1 Score: 186.8 bits (473), Expect = 7.600e-44
Identity = 86/179 (48.04%), Postives = 125/179 (69.83%), Query Frame = 0
Query:    5 VFKFIVVGNTDVGKSSLLLQFVEQRFNEKHAMTIGVEYGLKPAQVK-GRDIQIEIWDTAGQECYMALTRSYYRNADGCLVCFDLTNRSSFDSVSKWVEHARSNSGNPYLVLTLVGNKADCQGRRQVARAEAEALAAHHGMEYREITAKDNEAVQGLFHTTAERIFKAVESGPDGEAGAV 183
            +FK+I++G+T+VGKSS++L F EQ F + H MTIGVE+  +  Q+  G    +EIWDTAGQE Y+++TRSYYR  D CL+ +D+T R SFD + +W+  A+ N+ NP LVL LVGNKAD +G RQ++RAE +A A  HGM + E+TAK+   V+ +F  +A+R+     + P  ++ AV
Sbjct:   12 LFKYIIIGDTEVGKSSIMLMFTEQHFQQAHDMTIGVEFDSRSVQLSDGNQANLEIWDTAGQESYLSITRSYYRGTDCCLLVYDVTRRESFDHLPRWLNEAKQNASNPNLVLMLVGNKADLEGSRQISRAEGKAFADLHGMLFVELTAKNQNLVESVFVESAQRVLAVQRANPRAKSAAV 190          
BLAST of NO25G00350 vs. NCBI_GenBank
Match: XP_001453196.1 (hypothetical protein [Paramecium tetraurelia strain d4-2] >CAI39341.1 rab_B28 [Paramecium tetraurelia] >CAK85799.1 unnamed protein product (macronuclear) [Paramecium tetraurelia])

HSP 1 Score: 181.4 bits (459), Expect = 3.200e-42
Identity = 80/171 (46.78%), Postives = 129/171 (75.44%), Query Frame = 0
Query:    3 SLVFKFIVVGNTDVGKSSLLLQFVEQRFNEKHAMTIGVEYGLKPAQVKGRDIQIEIWDTAGQECYMALTRSYYRNADGCLVCFDLTNRSSFDSVSKWVEHARSNSGNPYLVLTLVGNKADCQGRRQVARAEAEALAAHHGMEYREITAKDNEAVQGLFHTTAERIFKAVES 174
            S +FKFI++G+T VGKS LLLQF+++RF +KH +TIGVE+G +  ++ G++I+++IWDTAGQE + ++TRSYYR+A G ++ +D+T R +F+++S+W+E A+ N GNP L  TLVGNK+D +  RQV+  E +  A +HG+++ E++AK    V+ +F  TA++I + + S
Sbjct:    4 SYLFKFILIGDTGVGKSCLLLQFIDKRFRQKHEVTIGVEFGARMIEIDGQNIKLQIWDTAGQESFRSITRSYYRSAAGAIIVYDITKRETFENISRWLEEAKQN-GNPKLTFTLVGNKSDLEADRQVSFEEGQEFARNHGVDFVEVSAKTANNVEEVFLKTAQQILEKINS 173          
BLAST of NO25G00350 vs. NCBI_GenBank
Match: XP_001441913.1 (hypothetical protein [Paramecium tetraurelia strain d4-2] >CAI43252.1 rab_A28 [Paramecium tetraurelia] >CAK74516.1 unnamed protein product (macronuclear) [Paramecium tetraurelia])

HSP 1 Score: 177.9 bits (450), Expect = 3.500e-41
Identity = 79/171 (46.20%), Postives = 129/171 (75.44%), Query Frame = 0
Query:    3 SLVFKFIVVGNTDVGKSSLLLQFVEQRFNEKHAMTIGVEYGLKPAQVKGRDIQIEIWDTAGQECYMALTRSYYRNADGCLVCFDLTNRSSFDSVSKWVEHARSNSGNPYLVLTLVGNKADCQGRRQVARAEAEALAAHHGMEYREITAKDNEAVQGLFHTTAERIFKAVES 174
            S +FKFI++G+T VGKS LLLQF+++RF +KH +TIGVE+G +  + +G++I+++IWDTAGQE + ++TRSYYR+A G ++ +D+T R +F+++S+W+E A+ N GNP L  TLVGNK+D +  RQV+  E +  A ++G+++ E++AK    V+ +F  TA++I + + S
Sbjct:    4 SYLFKFILIGDTGVGKSCLLLQFIDKRFRQKHEVTIGVEFGARMIEFEGQNIKLQIWDTAGQESFRSITRSYYRSAAGAIIVYDITKRETFENISRWLEEAKQN-GNPKLTFTLVGNKSDLEADRQVSFEEGQEFARNNGIDFVEVSAKTANNVEEVFLKTAQQILEKINS 173          
BLAST of NO25G00350 vs. NCBI_GenBank
Match: XP_022651518.1 (ras-related protein Rab-2A [Varroa destructor] >XP_022686419.1 ras-related protein Rab-2A [Varroa jacobsoni])

HSP 1 Score: 172.2 bits (435), Expect = 1.900e-39
Identity = 79/170 (46.47%), Postives = 120/170 (70.59%), Query Frame = 0
Query:    5 VFKFIVVGNTDVGKSSLLLQFVEQRFNEKHAMTIGVEYGLKPAQVKGRDIQIEIWDTAGQECYMALTRSYYRNADGCLVCFDLTNRSSFDSVSKWVEHARSNSGNPYLVLTLVGNKADCQGRRQVARAEAEALAAHHGMEYREITAKDNEAVQGLFHTTAERIFKAVESG 175
            +FK+I++G+T VGKS LLLQF ++RF   H +TIGVE+G +   +  + I+++IWDTAGQE + ++TRSYYR A G L+ +D+T R +FD ++ W+E AR +S N  +V+ L+GNK+D +GRRQV + E EA A  HG+ + E +AK  + V+  F  TA+ I++ +E G
Sbjct:    6 LFKYIIIGDTGVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMITIDRKQIKLQIWDTAGQEAFRSITRSYYRGAAGALLVYDITRRDTFDHLTTWLEDARQHS-NSNMVIMLIGNKSDLEGRRQVKKEEGEAFAQEHGLVFMETSAKTADNVEDAFIKTAKEIYEKIEEG 174          
BLAST of NO25G00350 vs. NCBI_GenBank
Match: ORX85488.1 (ras-domain-containing protein [Basidiobolus meristosporus CBS 931.73])

HSP 1 Score: 171.8 bits (434), Expect = 2.500e-39
Identity = 78/170 (45.88%), Postives = 122/170 (71.76%), Query Frame = 0
Query:    5 VFKFIVVGNTDVGKSSLLLQFVEQRFNEKHAMTIGVEYGLKPAQVKGRDIQIEIWDTAGQECYMALTRSYYRNADGCLVCFDLTNRSSFDSVSKWVEHARSNSGNPYLVLTLVGNKADCQGRRQVARAEAEALAAHHGMEYREITAKDNEAVQGLFHTTAERIFKAVESG 175
            +FK+I++G+T VGKS LLLQF ++RF   H +TIGVE+G +   V  + I++++WDTAGQE + ++TRSYYR A G L+ +D+T R +F+ ++KW+E AR +S N  + + L+GNK+D +G+R V++ E EA A  HG+ + E +AK +  V+  F  TAERI++ ++SG
Sbjct:    7 LFKYIIIGDTGVGKSCLLLQFTDKRFQPAHDLTIGVEFGARFITVDNKSIKLQVWDTAGQESFRSITRSYYRGAAGALLVYDITRRETFEHLAKWLEDAREHS-NDNITILLIGNKSDLEGKRAVSKEEGEAFAKEHGLFFLETSAKTSANVEEAFINTAERIYEKIQSG 175          
BLAST of NO25G00350 vs. NCBI_GenBank
Match: XP_003055718.1 (predicted protein [Micromonas pusilla CCMP1545] >EEH60970.1 predicted protein [Micromonas pusilla CCMP1545])

HSP 1 Score: 171.0 bits (432), Expect = 4.300e-39
Identity = 92/239 (38.49%), Postives = 137/239 (57.32%), Query Frame = 0
Query:    3 SLVFKFIVVGNTDVGKSSLLLQFVEQRFNEKHAMTIGVEYGLKPAQVKGRDIQIEIWDTAGQECYMALTRSYYRNADGCLVCFDLTNRSSFDSVSKWVEHARSNSGNPYLVLTLVGNKADCQGRRQVARAEAEALAAHHGMEYREITAKDNEAVQGLFHTTAERIFKAVESGPD----------GEAGAVGSDIVIDGAGRVXXXXXXXXXXXXXXGNIVLAPGKASVNPSSAAEGGCC 232
            S +FK+I++G+T VGKS LLLQF ++RF   H +TIGVE+G +   +  + I+++IWDTAGQE + ++TRSYYR A G L+ +D+T R +F+ ++ W+E AR ++ NP + +TLVGNK+D +  R V+  E EA A  HG+ + E +A+ +E V+ +F +TA  I   ++ G D          G  GA G  I   G G V                         V P+  +EGGCC
Sbjct:    4 SYLFKYIIIGDTGVGKSCLLLQFTDKRFQHVHDLTIGVEFGSRLVAIGEKQIKLQIWDTAGQESFRSITRSYYRGAAGALLVYDITRRDTFEHLTSWLEDARQHA-NPNMTVTLVGNKSDAEASRVVSTEEGEAFAREHGLAFLETSAQTSEGVEEVFTSTAAAICAKIDDGLDVNNESNGIKPGYGGARGGGIRSGGGGVV----------------------DVGVQPARRSEGGCC 219          
BLAST of NO25G00350 vs. NCBI_GenBank
Match: XP_018418650.1 (PREDICTED: ras-related protein Rab-2B [Nanorana parkeri])

HSP 1 Score: 169.5 bits (428), Expect = 1.200e-38
Identity = 78/170 (45.88%), Postives = 119/170 (70.00%), Query Frame = 0
Query:    5 VFKFIVVGNTDVGKSSLLLQFVEQRFNEKHAMTIGVEYGLKPAQVKGRDIQIEIWDTAGQECYMALTRSYYRNADGCLVCFDLTNRSSFDSVSKWVEHARSNSGNPYLVLTLVGNKADCQGRRQVARAEAEALAAHHGMEYREITAKDNEAVQGLFHTTAERIFKAVESG 175
            +FK+I++G+T VGKS LLLQF ++RF   H +TIGVE+G +   + G+ I+++IWDTAGQE + ++TRSYYR A G L+ +D+T R +F  ++ W+E AR +S +  +V+ L+GNK+D +GRR V+R E EA A  HG+ + E +AK    V+  F  TA+ I+K ++ G
Sbjct:    6 LFKYIIIGDTGVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMINIDGKPIKLQIWDTAGQESFRSITRSYYRGAAGALLVYDITRRETFSHLASWLEDARQHSSS-NMVIILIGNKSDLEGRRDVSREEGEAFAREHGLIFMETSAKTAANVEEAFIGTAKEIYKKIQQG 174          
BLAST of NO25G00350 vs. NCBI_GenBank
Match: XP_002646304.1 (C. briggsae CBR-UNC-108 protein [Caenorhabditis briggsae] >CAP31058.1 Protein CBR-UNC-108 [Caenorhabditis briggsae] >PIC51323.1 hypothetical protein B9Z55_001879 [Caenorhabditis nigoni])

HSP 1 Score: 169.5 bits (428), Expect = 1.200e-38
Identity = 77/170 (45.29%), Postives = 119/170 (70.00%), Query Frame = 0
Query:    5 VFKFIVVGNTDVGKSSLLLQFVEQRFNEKHAMTIGVEYGLKPAQVKGRDIQIEIWDTAGQECYMALTRSYYRNADGCLVCFDLTNRSSFDSVSKWVEHARSNSGNPYLVLTLVGNKADCQGRRQVARAEAEALAAHHGMEYREITAKDNEAVQGLFHTTAERIFKAVESG 175
            +FK+I++G+T VGKS LLLQF ++RF   H +TIGVE+G +   + G+ I+++IWDTAGQE + ++TRSYYR A G L+ +D+T R +F+ ++ W+E AR +S N  +V+ L+GNK+D + RR+V R E EA A  HG+ + E +AK    V+  F  TA+ I++ ++ G
Sbjct:    6 LFKYIIIGDTGVGKSCLLLQFTDKRFQPVHDLTIGVEFGARMVTIDGKQIKLQIWDTAGQESFRSITRSYYRGAAGALLVYDITRRDTFNHLTSWLEDARQHS-NSNMVIMLIGNKSDLEARREVKREEGEAFAREHGLVFMETSAKTANNVEEAFIDTAKEIYRKIQEG 174          
The following BLAST results are available for this feature:
BLAST of NO25G00350 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes)
Total hits: 20
Match NameE-valueIdentityDescription
EWM21552.13.200e-5861.31rab family gtpase [Nannochloropsis gaditana][more]
CBN76817.13.400e-4446.70Rab2B, RAB family GTPase [Ectocarpus siliculosus][more]
XP_009034590.17.600e-4448.04hypothetical protein AURANDRAFT_22559 [Aureococcus... [more]
XP_001453196.13.200e-4246.78hypothetical protein [Paramecium tetraurelia strai... [more]
XP_001441913.13.500e-4146.20hypothetical protein [Paramecium tetraurelia strai... [more]
XP_022651518.11.900e-3946.47ras-related protein Rab-2A [Varroa destructor] >XP... [more]
ORX85488.12.500e-3945.88ras-domain-containing protein [Basidiobolus merist... [more]
XP_003055718.14.300e-3938.49predicted protein [Micromonas pusilla CCMP1545] >E... [more]
XP_018418650.11.200e-3845.88PREDICTED: ras-related protein Rab-2B [Nanorana pa... [more]
XP_002646304.11.200e-3845.29C. briggsae CBR-UNC-108 protein [Caenorhabditis br... [more]

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Relationships

This gene is member of the following syntenic_region feature(s):

Feature NameUnique NameSpeciesType
nonsL046nonsL046Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
nonsL045nonsL045Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ncniR043ncniR043Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ngnoR073ngnoR073Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region


This gene is orthologous to the following gene feature(s):

Feature NameUnique NameSpeciesType
NSK004390NSK004390Nannochloropsis salina (N. salina CCMP1776)gene


The following polypeptide feature(s) derives from this gene:

Feature NameUnique NameSpeciesType
NO25G00350.1NO25G00350.1-proteinNannochloropsis oceanica (N. oceanica IMET1)polypeptide


The following gene feature(s) are orthologous to this gene:

Feature NameUnique NameSpeciesType
jgi.p|Nanoce1779_2|602933gene_8517Nannochloropsis oceanica (N. oceanica CCMP1779)gene
Rab2Bgene8877Nannochloropsis gaditana (N. gaditana B-31)gene


The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
NO25G00350.1NO25G00350.1Nannochloropsis oceanica (N. oceanica IMET1)mRNA


Sequences
The following sequences are available for this feature:

gene sequence

>NO25G00350 ID=NO25G00350|Name=NO25G00350|organism=Nannochloropsis oceanica|type=gene|length=1282bp
ATGCCATgtaagtgatagataggggagcaatggataacatcaagccatac
gttttttaaagtggcctctggaaaaaaacaacgcaacacctctgcaccac
caaacgcagCGCTCGTCTTCAAATTCATCGTGGTTGGGAACACGGATGTC
GGCAAGTCCTCACTCCTCCTTCAGTTTGTAGAACAGCGATTTAATGAGAA
ACATGCCATGACGgtacgctttattggagagattagatgtttaaataggg
attatctgcgagagcttgtcctagacgtatgtcgtgctccataattctgc
ctctttcgttcccttgctctgtccaccgttcaaccatgtccatcgctcgg
ttcaaagccactttgtttttatattcctcatccgtatattgtagATCGGC
GTAGAGTATGGGCTGAAGCCGGCACAAGTCAAGGGCCGCGACATCCAGAT
CGAGATTTGGGACACGGCCGGGCAAGAGTGCTACATGGCTTTGACACGCT
Cgtaagctagcatggcaggaagaggtttagtctgggaagaggaagcagtc
gggcatcctgagcttagattccatgtatttcatgcttatggaaccgttgt
atctttgcttgtgtatcgtgctcgttgcccgtccccagctactcataggc
ccacccctcctcccccaacagGTACTACCGGAATGCCGACGGCTGTCTGG
TCTGCTTCGATTTGACAAATAGAAGCAGTTTCGACAGTGTCTCCAAATGG
GTTGAACACGCTCGCAGCAACAGCGGTAACCCTTACCTCGTGCTCACGCT
GGTTGGCAACAAGGCCGACTGCCAGGGGAGACGACAAGTGGCGCGGGCGG
AGGCGGAGGCCTTGGCAGCCCATCACGGCATGGAGTgtaagtgggcggtt
gagatgcttttgtaatgctgtttcactgatgcccttcctccctcgctcgc
tttctggcggccgcacggtttttggtcagtgtcattcttagcctccctcc
tttccgctcttcctcgcagACCGAGAAATCACTGCCAAGGATAATGAGGC
CGTGCAGGGGCTCTTCCACACCACCGCAGAGCGGATCTTCAAGGCAGTCG
AGAGTGGGCCGGATGGAGAGGCGGGGGCGGTGGGAAGCGATATTGTCATC
GACGGAGCCGGTCGAGTCCGCCTCCGTTCCACCTCTTCCTCTTCCGCTTC
CTCTTCCACCGGGAACATTGTGCTCGCTCCAGGAAAGGCCAGCGTAAATC
CAAGCAGTGCCGCAGAAGGCGGTTGCTGCTAG
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protein sequence of NO25G00350.1

>NO25G00350.1-protein ID=NO25G00350.1-protein|Name=NO25G00350.1|organism=Nannochloropsis oceanica|type=polypeptide|length=232bp
MPSLVFKFIVVGNTDVGKSSLLLQFVEQRFNEKHAMTIGVEYGLKPAQVK
GRDIQIEIWDTAGQECYMALTRSYYRNADGCLVCFDLTNRSSFDSVSKWV
EHARSNSGNPYLVLTLVGNKADCQGRRQVARAEAEALAAHHGMEYREITA
KDNEAVQGLFHTTAERIFKAVESGPDGEAGAVGSDIVIDGAGRVRLRSTS
SSSASSSTGNIVLAPGKASVNPSSAAEGGCC*
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Synonyms
Publications