NO19G01220, NO19G01220 (gene) Nannochloropsis oceanica

BLAST of NO19G01220 vs. NCBI_GenBank
Match: EWM23648.1 (trna (uracil-5-)-methyltransferase [Nannochloropsis gaditana])

HSP 1 Score: 510.0 bits (1312), Expect = 1.100e-140
Identity = 310/636 (48.74%), Postives = 370/636 (58.18%), Query Frame = 0

Query:    9 EEDADGILLLQHDTLGTDLSYLCLDDYSHEQYEVSLQGKVQALRELFQPYWSSVLTVFRSPPVHHRLRAKFGIGRVITRNEGSQXXXXXXXXXXXXXXLEKISEGTAANTMTSSKLCYLMWGYDQRPVAISSFPLASRRINDLMPRLLQALEAGPVVLRRGLRAVNFLDTLAGDTLISLMYQVPLIEGGKEGGQGGFWAEAAGIWLRDGLGCQVVGRSKGRVVAVDRAYVTEAFHLF---PCSSSSSSPCFSSFYLGNNCEGKGDKARKAEELVSFFPPPSSSPHPSPCSHIPVPTILRYKQWEGAFSNPNGQMAMHTIRWLCARADAILLAADEAK------TEADEEGGNRA-----------HEGLGGCGESKTGSDKXXXXXXXXXXXXXXXXXXXRQNCNHHSTKSTCDLLELYCGGGNHTVALSRKFQRVVGVEIDKRLVAVARENLVLNGIENTTIIQIPSEKFRVEELGKYRRK------EREKERKAAAAVVPGEVEGMKVGDCGNSKEVEXXXXXXXXXXXXXXXXXXXFNFGVVLVDPPRVGLDRHTRKLISQYEHILYISCGPHSMLRDLQGEDEEKQERSTDKTYRSLAGFGLVETHDVVDMCVLDHFPGTMHIEAAVHLRRR 619
            E   + IL L  + LG DLSYL  +  S +QYE SLQ KV  LR +F PYWS   T+FRSPPVH+RLRAKFGIGRV+ +                       S  T A   +  KLCYLMWG D++ V ISSFPLASRRINDLMPRLL AL  GP++LRR LRAVNFLDTLAGDTLI+LMY+    EGGK   +   W  AA   +++ LG QVVGR K   + VDRAYV E F L    P SS  +SP    +  G      G   R         P  +S+  P PC     P+ILRYKQW+GAFSNPNG MA+HT+RWLCARADA+L   D  +       EADE G  R             EG GG G  +   D                     +           DLLEL+CGGGNHTVALSRKF+RVVGVEID+RLVA A+ENL LNG++N ++IQIPSE+F  E LG YRR+                 + PG+      G+ G +   +                    +F VVLVDPPR GLDRHTRK++S+YE ILYISC P S+LRDL+GE EE++ +S D+ + SLAGFGLV TH VVDMCVLDHFPGT HIE AVHL+RR
Sbjct:    5 EGRCEDILTLAGEALGIDLSYLSPNSASLDQYETSLQRKVDELRSIFFPYWSCEPTIFRSPPVHYRLRAKFGIGRVLPQASHPAGPMAGITE----------SARTGAPQGSPGKLCYLMWGADKKLVVISSFPLASRRINDLMPRLLLALGEGPLILRRSLRAVNFLDTLAGDTLIALMYETAWAEGGKAAKEE--WVAAAREHVKEALGVQVVGRGKRVALPVDRAYVLEEFCLSPPPPASSRGASP-LPPYAPGTGGADTGRDPRGG-------PRRASAGVPHPC-----PSILRYKQWDGAFSNPNGCMALHTLRWLCARADAVLQRGDAPRGVQATGGEADERGRERQMEDGDREQQGDTEGRGGPGGCEGCVDVASGGEGRVCRERQGLRPRVCRPPPSGDKPPAFDLLELFCGGGNHTVALSRKFRRVVGVEIDRRLVAAAKENLALNGVKNASVIQIPSERFHPERLGHYRRQXXXXXXXXXXXXXXXXMLGPGKRVASAGGEAGLTARAQGMGQSPPGEDDP--------SFRVVLVDPPRAGLDRHTRKMVSKYEDILYISCAPLSLLRDLRGESEEERRQSADRKFLSLAGFGLVHTHKVVDMCVLDHFPGTRHIEVAVHLQRR 607          

BLAST of NO19G01220 vs. NCBI_GenBank
Match: XP_005854838.1 (trna (uracil-5-)-methyltransferase, partial [Nannochloropsis gaditana CCMP526] >EKU21518.1 trna (uracil-5-)-methyltransferase, partial [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 198.7 bits (504), Expect = 5.200e-47
Identity = 107/189 (56.61%), Postives = 125/189 (66.14%), Query Frame = 0

Query:    9 EEDADGILLLQHDTLGTDLSYLCLDDYSHEQYEVSLQGKVQALRELFQPYWSSVLTVFRSPPVHHRLRAKFGIGRVITRNEGSQXXXXXXXXXXXXXXLEKISEGTAANTMTSSKLCYLMWGYDQRPVAISSFPLASRRINDLMPRLLQALEAGPVVLRRGLRAVNFLDTLAGDTLISLMYQVPLIEGG 198
            E   + IL+L  + LG DLSYL  +  S +QYE SLQ KV  LR +F PYWS   T+FRSPPVH+RLRAKFGIGRV+ +                       S  T A   +  KLCYLMWG D++PV ISSFPLASRRINDLMPRLL AL  GP++LRR LRAVNFLDTLAGDTLI+L+Y+    EGG
Sbjct:    5 EGRCEDILMLAGEALGIDLSYLSPNSASLDQYETSLQRKVDELRSIFFPYWSCQPTIFRSPPVHYRLRAKFGIGRVLPQASHPAGPMAGITE----------SARTGAPQGSPGKLCYLMWGADKKPVVISSFPLASRRINDLMPRLLLALGEGPLILRRSLRAVNFLDTLAGDTLIALIYETAWAEGG 183          

BLAST of NO19G01220 vs. NCBI_GenBank
Match: XP_002500539.1 (predicted protein [Micromonas commoda] >ACO61797.1 predicted protein [Micromonas commoda])

HSP 1 Score: 179.1 bits (453), Expect = 4.300e-41
Identity = 176/638 (27.59%), Postives = 233/638 (36.52%), Query Frame = 0

Query:   33 DDYSHEQYEVSLQGKVQALRELFQPYWSSVL-----------------TVFRSPPVHHRLRAKFGIGRVITRNEGSQXXXXXXXXXXXXXXLEKISEGTAANTMTSSKLCYLMWGYDQRPVAISSFPLASRRINDLMPRLLQALEAGPVVLRRGLRAVNFLDT--LAGDTLISLMYQVPLIE--------------------------GGKEGGQGGFWAEAAGIWLRDGLGCQVVGRSKG-RVVAVDRAYVTEAFHLFPCSSSSSSPCFSSFYLGNNCEGKGDKARKAEELVSFFPPPSSSPHPSPCSHIPVPTILRYKQWEGAFSNPNGQMAMHTIRWLCARADAILLAADEAKTEADEEGGNRAHEGLGGCGESKTGSDKXXXXXXXXXXXXXXXXXXXRQNCNHHSTKSTCDLLELYCGGGNHTVALSRKFQRVVGVEIDKRLVAVARENLVLNGIENTTIIQIPSEKFRVEELGKYRRKEREKE----RKAAAAVVPGEVEGMKVGDCGNSKEVEXXXXXXXXXXXXXXXXXXXFNFGVVLVDPPRVGLDRHTRKLISQYEHILYISCGPHSMLRDLQGEDEEKQERSTDKTYRSLAGF-GLVETHDVVDMCVLDHFPGTMHIEAAVHLRRRR 620
            D      Y  +L  KV  L  LF P                        VF SPP++ R R +F +                          E        +     +L Y MW   +  V I+ FP+A R I +LMP +L+  EA P  L  GL+A++FL +  + G  L++++Y  P+ E                          GG + G  GF          D     ++GRS+G ++V  D  +V E   L                         D  R                             LRY+Q EGAFSNPN  MA  T+ WLC  A                       E LGG  E                               H +  +   LLELY G GNHTVAL++ F  V  VE+   L   ARENL  NG+EN  ++  PSE       G  RRK+R +E    + A AA   G  +G K GD G+  E +                     + VVLVDPPR GLD  T  L+S+++ +LYISC P   L +        +   T +      G+ GL  THD+V   V DHFP T HIE      RRR
Sbjct:  648 DPVDASTYSAALDAKVAELERLFSPLMQQASIPILNAKGVDRSVSVRPEVFPSPPLNFRSRCRFQV-----------------MWEDAPVTAESTGITPGESVEPGRRLSYRMWNKGKPTVPITEFPMALREIEELMPAVLREAEAHP-ELGDGLQAIHFLASRGIRGGMLVTMVYGRPIDEAWKMAAEGPRARLKAAMVDIRERVYGGGDDDGFDGFAGPRGDDEELDATALNILGRSRGTQIVVGDACHVFETLAL------------------------RDGRR-----------------------------LRYRQAEGAFSNPNSFMAESTLDWLCDCA------------------ATTVREALGGTSE-------------------------------HSAEAAPPSLLELYSGNGNHTVALAKYFDSVAAVELSGVLCEAARENLERNGVENAKVLHSPSESV---ARGMLRRKKRHEEGRVGKAAMAAAATGAGDGSKRGD-GSGMEFDINA------------------YDVVLVDPPRAGLDPDTLDLVSRFDVVLYISCDPRKGLLENAVGPHAARALDTGEFEGEFKGWSGLARTHDLVRFAVFDHFPYTRHIECGACFVRRR 1143          

BLAST of NO19G01220 vs. NCBI_GenBank
Match: WP_078085881.1 (tRNA (uridine(54)-C5)-methyltransferase TrmA [Microbulbifer mangrovi])

HSP 1 Score: 168.7 bits (426), Expect = 5.800e-38
Identity = 156/593 (26.31%), Postives = 229/593 (38.62%), Query Frame = 0

Query:   30 LCLDDYSHEQYEVSLQGKVQALRELFQPYWSSVLTVFRSPPVHHRLRAKFGIGRVITRNEGSQXXXXXXXXXXXXXXLEKISEGTAANTMTSSKLCYLMW--GYDQRPVAISSFPLASRRINDLMPRLLQALEAGPVVLRRGLRAVNFLDTLAGDTLISLMYQVPLIEGGKEGGQGGFWAEAAGIWLRDGLGCQVVGRSKGRVVAVDRAYVTEAFHLFPCSSSSSSPCFSSFYLGNNCEGKGDKARKAEELVSFFPPPSSSPHPSPCSHIPVPTILRYKQWEGAFSNPNGQMAMHTIRWLCARADAILLAADEAKTEADEEGGNRAHEGLGGCGESKTGSDKXXXXXXXXXXXXXXXXXXXRQNCNHHSTKSTCDLLELYCGGGNHTVALSRKFQRVVGVEIDKRLVAVARENLVLNGIENTTIIQIPSEKF--RVEELGKYRRKEREKERKAAAAVVPGEVEGMKVGDCGNSKEVEXXXXXXXXXXXXXXXXXXXFNFGVVLVDPPRVGLDRHTRKLISQYEHILYISCGPHSMLRDLQGEDEEKQERSTDKTYRSLAGFGLVETHDVVDMCVLDHFPGTMHIEAAVHLRRR 619
            + L   + + Y+  L  KV  LR  F P+ S    VF SPP H+RLRA+F + +                            EG         ++ Y M+  G  ++P  I  F + SRRIN LMP LL+A+     +LR+ L    FL TL+GD LI+L+Y  PL E          W + A   L+D LG  ++GRS+ + + +DR YV E   +                     +G+                                   +YKQ EG+F+ PNG +    I+W  AR+                            C E   G                                   DLLELYCG GN T+ L+ KF++V+  EI K  V  A+EN+  NG++N  I+++ SE+F   ++++  +RR                 ++G+ +                             ++F  VLVDPPR GLD  T  +I+++  ILYISC P + L +L+                      L +TH V    + D FP T H E+AV L RR
Sbjct:    1 MALHRVNTDDYQQQLDQKVDRLRGAFSPFTSLAPEVFPSPPSHYRLRAEFKMWQ----------------------------EG--------GRVDYAMYKQGEYKKPFVIQEFTVGSRRINALMPPLLEAINDSE-ILRKRLFCTEFLTTLSGDALITLIYHKPLDEA---------WEQEARA-LQDHLGVPILGRSRKQKLVLDRDYVIEKLEI---------------------DGRS---------------------------------YQYKQVEGSFTQPNGDICTSMIQW--ARS----------------------------CCEDTEG-----------------------------------DLLELYCGNGNFTIPLAEKFRKVLATEISKVSVNAAQENIAANGVDNLDIVRLSSEEFTQAIDKVRPFRR-----------------LKGVDL---------------------------ESYDFSTVLVDPPRAGLDADTCAMIARFPRILYISCNPQTQLENLE---------------------ELTKTHKVERFAIFDQFPYTDHTESAVLLVRR 362          

BLAST of NO19G01220 vs. NCBI_GenBank
Match: WP_010130808.1 (tRNA (uridine(54)-C5)-methyltransferase TrmA [Microbulbifer agarilyticus])

HSP 1 Score: 161.0 bits (406), Expect = 1.200e-35
Identity = 149/591 (25.21%), Postives = 223/591 (37.73%), Query Frame = 0

Query:   30 LCLDDYSHEQYEVSLQGKVQALRELFQPYWSSVLTVFRSPPVHHRLRAKFGIGRVITRNEGSQXXXXXXXXXXXXXXLEKISEGTAANTMTSSKLCYLMW--GYDQRPVAISSFPLASRRINDLMPRLLQALEAGPVVLRRGLRAVNFLDTLAGDTLISLMYQVPLIEGGKEGGQGGFWAEAAGIWLRDGLGCQVVGRSKGRVVAVDRAYVTEAFHLFPCSSSSSSPCFSSFYLGNNCEGKGDKARKAEELVSFFPPPSSSPHPSPCSHIPVPTILRYKQWEGAFSNPNGQMAMHTIRWLCARADAILLAADEAKTEADEEGGNRAHEGLGGCGESKTGSDKXXXXXXXXXXXXXXXXXXXRQNCNHHSTKSTCDLLELYCGGGNHTVALSRKFQRVVGVEIDKRLVAVARENLVLNGIENTTIIQIPSEKFRVEELGKYRRKEREKERKAAAAVVPGEVEGMKVGDCGNSKEVEXXXXXXXXXXXXXXXXXXXFNFGVVLVDPPRVGLDRHTRKLISQYEHILYISCGPHSMLRDLQGEDEEKQERSTDKTYRSLAGFGLVETHDVVDMCVLDHFPGTMHIEAAVHLRRR 619
            + L   + ++Y+  L  KV  LRE + PY      VF SPP H+RLRA+F + +                                       ++ Y M+  G  ++P  I  F + SRRIN+LMP LL A+ A   +LR+ L    FL TL+GD LI+L+Y  PL         G  W E+    L++ LG  V+GRS+ + + ++R YV E   +                     +G+                                 + +YKQ EG+F+ PNG++    I W              AK+   + G  +A                                              + DLLELYCG GN T+ L+  F++ +  EI K  V  A+EN+  NG++N  I+++ SE+F  + + K R   R K+                                              ++F  VLVDPPR GLD+ T  +I ++  ILYISC P + L +L                       L +TH +    + D FP T H E+ V L R+
Sbjct:    1 MALHRVNTDEYQQQLDQKVARLREAYTPYTELAPEVFPSPPSHYRLRAEFKMWQ------------------------------------DGGRVDYAMYKQGEYKKPFVIQEFTVGSRRINELMPPLLDAINASE-ILRKRLFCTEFLTTLSGDALITLIYHKPL---------GDEW-ESEARALQELLGVPVLGRSRKQKLVLERDYVIEKLDI---------------------DGR---------------------------------VFQYKQVEGSFTQPNGEICRSMISW--------------AKSCCPQPGAEQA----------------------------------------------SGDLLELYCGNGNFTIPLASHFRKALATEISKVSVNAAQENIAANGVDNLAILRLSSEEF-TQAIDKLRPFRRLKD-----------------------------------------FDLDSYDFSTVLVDPPRAGLDQDTCAMIVRFPRILYISCNPETQLENLA---------------------ELTKTHRIERFAIFDQFPYTDHTESGVLLVRK 367          

BLAST of NO19G01220 vs. NCBI_GenBank
Match: WP_027021434.1 (tRNA (uridine(54)-C5)-methyltransferase TrmA [Conchiformibius steedae])

HSP 1 Score: 159.8 bits (403), Expect = 2.700e-35
Identity = 155/587 (26.41%), Postives = 216/587 (36.80%), Query Frame = 0

Query:   37 HEQYEVSLQGKVQALRELFQPYWSSVLTVFRSPPVHHRLRAKFGIGRVITRNEGSQXXXXXXXXXXXXXXLEKISEGTAANTMTSSKLCYLMWGYDQRP-----VAISSFPLASRRINDLMPRLLQALEAGPVVLRRGLRAVNFLDTLAGDTLISLMYQVPLIEGGKEGGQGGFWAEAAGIWLRDGLGCQVVGRSKGRVVAVDRAYVTEAFHLFPCSSSSSSPCFSSFYLGNNCEGKGDKARKAEELVSFFPPPSSSPHPSPCSHIPVPTILRYKQWEGAFSNPNGQMAMHTIRWLCARADAILLAADEAKTEADEEGGNRAHEGLGGCGESKTGSDKXXXXXXXXXXXXXXXXXXXRQNCNHHSTKSTCDLLELYCGGGNHTVALSRKFQRVVGVEIDKRLVAVARENLVLNGIENTTIIQIPSEKFRVEELGKYRRKEREKERKAAAAVVPGEVEGMKVGDCGNSKEVEXXXXXXXXXXXXXXXXXXXFNFGVVLVDPPRVGLDRHTRKLISQYEHILYISCGPHSMLRDLQGEDEEKQERSTDKTYRSLAGFGLVETHDVVDMCVLDHFPGTMHIEAAVHLRRR 619
            H  Y   L  K   L+ L  P+ +    VF SPP H+R+RA+F I                                         ++CY M+   Q+      + +  FP A+  IN LMPRLL A+  G  VLR  L    FL TL+GD LI+L+Y   L         G  W  AA   L+D LG  ++GRS+G+ + + R +VTE+  +                        G+                                L Y+Q EG FS PN  +    + W          A+D A             + LGG                                          DLLELYCG GN TV LSR+F+RV+  E+ K  V  A+ NL  N  +N  + ++ +     EEL +    ER   R A         + + + D                           F+F  V VDPPR G+D  T KL++ +++ILYISC PHS+  +L+                      L  TH VV   + D FP T HIE  V+L+R+
Sbjct:    2 HSDYSAQLAEKTARLQNLLAPFDAPAPEVFASPPQHYRMRAEFRIWH------------------------------------DGDEICYAMFERGQKAATASLIRLQQFPAAAESINALMPRLLAAVR-GDAVLRERLYQCEFLATLSGDMLITLIYHKKL---------GDDWT-AAAQRLQDDLGIALIGRSRGQKIVLKRDFVTESLKV-----------------------NGE-------------------------------TLHYRQIEGGFSQPNALVCEKMLAW----------ASDCA-------------QNLGG------------------------------------------DLLELYCGNGNFTVPLSRRFRRVLATELSKTSVKAAQWNLATNSADNVQLARLSA-----EELAQAFAGERVFRRLAE--------QNIDLSD---------------------------FDFSTVFVDPPRAGVDSDTLKLLADFDNILYISCNPHSLADNLK---------------------QLCATHQVVRSALFDQFPFTEHIECGVYLQRK 361          

BLAST of NO19G01220 vs. NCBI_GenBank
Match: WP_077403755.1 (tRNA (uridine(54)-C5)-methyltransferase TrmA [Microbulbifer agarilyticus] >AQQ67774.1 tRNA (uridine(54)-C5)-methyltransferase TrmA [Microbulbifer agarilyticus])

HSP 1 Score: 159.5 bits (402), Expect = 3.500e-35
Identity = 147/591 (24.87%), Postives = 223/591 (37.73%), Query Frame = 0

Query:   30 LCLDDYSHEQYEVSLQGKVQALRELFQPYWSSVLTVFRSPPVHHRLRAKFGIGRVITRNEGSQXXXXXXXXXXXXXXLEKISEGTAANTMTSSKLCYLMW--GYDQRPVAISSFPLASRRINDLMPRLLQALEAGPVVLRRGLRAVNFLDTLAGDTLISLMYQVPLIEGGKEGGQGGFWAEAAGIWLRDGLGCQVVGRSKGRVVAVDRAYVTEAFHLFPCSSSSSSPCFSSFYLGNNCEGKGDKARKAEELVSFFPPPSSSPHPSPCSHIPVPTILRYKQWEGAFSNPNGQMAMHTIRWLCARADAILLAADEAKTEADEEGGNRAHEGLGGCGESKTGSDKXXXXXXXXXXXXXXXXXXXRQNCNHHSTKSTCDLLELYCGGGNHTVALSRKFQRVVGVEIDKRLVAVARENLVLNGIENTTIIQIPSEKFRVEELGKYRRKEREKERKAAAAVVPGEVEGMKVGDCGNSKEVEXXXXXXXXXXXXXXXXXXXFNFGVVLVDPPRVGLDRHTRKLISQYEHILYISCGPHSMLRDLQGEDEEKQERSTDKTYRSLAGFGLVETHDVVDMCVLDHFPGTMHIEAAVHLRRR 619
            + L   + ++Y+  L  KV  LRE + PY      VF SPP H+R+RA+F + +                                       ++ Y M+  G  ++P  I  F + SRRIN+LMP LL A+ A   +LR+ L    FL TL+GD LI+L+Y  PL         G  W E+    L++ LG  ++GRS+ + + ++R YV E   +                     +G+                                   +YKQ EG+F+ PNG++    I W           A     +  E+                                                 K++ DLLELYCG GN T+ L+  F++V+  EI K  V  A+EN+  NG++N  I+++ SE+F  + + K R   R K+                                              ++F  VLVDPPR GLD+ T  +I+++  ILYISC P + L +L                       L +TH +    + D FP T H E+ V L R+
Sbjct:    1 MALHRVNTDEYQQQLDQKVARLREAYTPYTDLAPEVFPSPPSHYRMRAEFKMWQ------------------------------------DGGRVDYAMYKQGEYKKPFVIQEFTVGSRRINELMPPLLDAINASE-MLRKRLFCTEFLTTLSGDALITLIYHKPL---------GDEW-ESEARALQEQLGVPILGRSRKQKLVLERDYVIEKLDI---------------------DGRE---------------------------------YQYKQVEGSFTQPNGEICRSMISW-----------AKSCCPQTSED-------------------------------------------------KASGDLLELYCGNGNFTIPLAAHFRKVLATEISKVSVNAAQENIAANGVDNLAILRLSSEEF-TQAIDKVRPFRRLKD-----------------------------------------FDLDSYDFSTVLVDPPRAGLDQDTCAMIARFPKILYISCNPETQLENLA---------------------ELTKTHRIERFAIFDQFPYTDHTESGVLLVRK 367          

BLAST of NO19G01220 vs. NCBI_GenBank
Match: WP_015046129.1 (tRNA (uridine(54)-C5)-methyltransferase TrmA [Simiduia agarivorans] >AFU97956.1 tRNA (uracil-5-)-methyltransferase [Simiduia agarivorans SA1 = DSM 21679])

HSP 1 Score: 158.3 bits (399), Expect = 7.800e-35
Identity = 152/583 (26.07%), Postives = 204/583 (34.99%), Query Frame = 0

Query:   36 SHEQYEVSLQGKVQALRELFQPYWSSVLTVFRSPPVHHRLRAKFGIGRVITRNEGSQXXXXXXXXXXXXXXLEKISEGTAANTMTSSKLCYLMWGYDQRPVAISSFPLASRRINDLMPRLLQALEAGPVVLRRGLRAVNFLDTLAGDTLISLMYQVPLIEGGKEGGQGGFWAEAAGIWLRDGLGCQVVGRSKGRVVAVDRAYVTEAFHLFPCSSSSSSPCFSSFYLGNNCEGKGDKARKAEELVSFFPPPSSSPHPSPCSHIPVPTILRYKQWEGAFSNPNGQMAMHTIRWLCARADAILLAADEAKTEADEEGGNRAHEGLGGCGESKTGSDKXXXXXXXXXXXXXXXXXXXRQNCNHHSTKSTCDLLELYCGGGNHTVALSRKFQRVVGVEIDKRLVAVARENLVLNGIENTTIIQIPSEKFRVEELGKYRRKEREKERKAAAAVVPGEVEGMKVGDCGNSKEVEXXXXXXXXXXXXXXXXXXXFNFGVVLVDPPRVGLDRHTRKLISQYEHILYISCGPHSMLRDLQGEDEEKQERSTDKTYRSLAGFGLVETHDVVDMCVLDHFPGTMHIEAAVHLRRR 619
            S E Y+  L  KV  +   F P     + VF SP  H+RLRA+F I +                            +G A   M          G  ++P  I  FP+ + RIN LMPRLL  + A    LR+ L +  FL TL+GD LI+L+Y  PL E          W +AA + LR  LG  ++GRS+ + + ++R YV E                       + +GK                                    YKQ EG F+ PNG +    + W  A                       A +G  G                                          DLLELYCG GN T+ L++ F RV+  EI K  V  A  N  LN IEN  I ++ SE+F  + + K R   R                                                 ++F  + VDPPR GLD  T  L  ++E I+YISC PH++L +LQ                     GL  +H V    V D FP T H+E    L RR
Sbjct:    7 SPEHYDDQLADKVATIERAFAPLDIPAIEVFDSPKSHYRLRAEFKIWQ---------------------------EDGRAHYAMYEK-------GEYKKPYIIDGFPVGAERINQLMPRLLAEINANE-TLRKKLFSAEFLTTLSGDALITLLYHKPLDEQ---------WEQAARV-LRTSLGVDLIGRSRKQKITLERDYVNETL---------------------SVDGKP---------------------------------FHYKQIEGGFTQPNGAVCEKMLAWAVA-----------------------ASKGCSG------------------------------------------DLLELYCGNGNFTLPLAQNFDRVLATEISKTSVEAALHNAELNRIENVAIARMSSEEF-TQAMDKVRPFRR-----------------------------------------LAHIDLDSYHFSTLFVDPPRAGLDPDTLALAQRFERIIYISCNPHTLLDNLQ---------------------GLSNSHKVDRFAVFDQFPYTDHLECGAILSRR 362          

BLAST of NO19G01220 vs. NCBI_GenBank
Match: WP_044498537.1 (tRNA (uridine(54)-C5)-methyltransferase TrmA [Pseudomonas saudimassiliensis] >CEA02915.1 tRNA (uracil-5-)-methyltransferase [Pseudomonas saudimassiliensis] >CEF26004.1 tRNA (uracil-5-)-methyltransferase [Pseudomonas saudimassiliensis])

HSP 1 Score: 157.5 bits (397), Expect = 1.300e-34
Identity = 152/579 (26.25%), Postives = 218/579 (37.65%), Query Frame = 0

Query:   40 YEVSLQGKVQALRELFQPYWSSVLTVFRSPPVHHRLRAKFGIGRVITRNEGSQXXXXXXXXXXXXXXLEKISEGTAANTMTSSKLCYLMWGYDQRPVAISSFPLASRRINDLMPRLLQALEAGPVVLRRGLRAVNFLDTLAGDTLISLMYQVPLIEGGKEGGQGGFWAEAAGIWLRDGLGCQVVGRSKGRVVAVDRAYVTEAFHLFPCSSSSSSPCFSSFYLGNNCEGKGDKARKAEELVSFFPPPSSSPHPSPCSHIPVPTILRYKQWEGAFSNPNGQMAMHTIRWLCARADAILLAADEAKTEADEEGGNRAHEGLGGCGESKTGSDKXXXXXXXXXXXXXXXXXXXRQNCNHHSTKSTCDLLELYCGGGNHTVALSRKFQRVVGVEIDKRLVAVARENLVLNGIENTTIIQIPSEKFRVEELGKYRRKEREKERKAAAAVVPGEVEGMKVGDCGNSKEVEXXXXXXXXXXXXXXXXXXXFNFGVVLVDPPRVGLDRHTRKLISQYEHILYISCGPHSMLRDLQGEDEEKQERSTDKTYRSLAGFGLVETHDVVDMCVLDHFPGTMHIEAAVHLRRR 619
            YE  L+ K Q L  L QP+ +    VF SP  H RLRA+F +                              +G     M          G ++RP+ I   P+AS+RIN+LM  LL+A +A P VL R L  + FL TL+G+ L++L Y  P+ E          W  AA + L + LG  ++GRS+GR   + R +V EA  +                                                            Y+Q EG F+ PNGQ+  H + W      A+  A D ++                                                           DLLELYCG GN T+ LS +F+RV+  E+ K  V  A EN+ LN ++N T++++ S     EE+ +     RE  R          ++G+ +                             ++FG V VDPPR GLD  T +L+  Y+ ILYISC PH++  +L   DE                  L  TH +V   + D FP + H+E+ V L RR
Sbjct:   10 YEQQLEEKRQRLIALLQPFAAPEPEVFASPREHFRLRAEFRLW---------------------------YEQGQPHYAMFEP-------GDNRRPILIEQLPIASKRINELMMPLLEACKADP-VLGRKLFQIEFLTTLSGEALVTLCYHRPIDED---------WDVAATV-LAEQLGILLIGRSRGRKRVLQRDHVLEALTV------------------------------------------------------AGRTWHYQQAEGGFTQPNGQVNQHMLAW------ALDAAGDNSR-----------------------------------------------------------DLLELYCGNGNFTLPLSTRFRRVLATELSKSSVRSALENIALNQVDNVTLVRLAS-----EEVTQALTGVREFRR----------LQGVDLS---------------------------GYDFGTVFVDPPRAGLDPATLELVKGYDRILYISCNPHTLAANL---DE------------------LHGTHRIVRCALFDQFPYSHHMESGVLLERR 361          

BLAST of NO19G01220 vs. NCBI_GenBank
Match: WP_061900804.1 (tRNA (uridine(54)-C5)-methyltransferase TrmA [Vibrio cidicii] >KYN81821.1 tRNA (uracil-5-)-methyltransferase [Vibrio cidicii])

HSP 1 Score: 157.5 bits (397), Expect = 1.300e-34
Identity = 153/590 (25.93%), Postives = 222/590 (37.63%), Query Frame = 0

Query:   34 DYSHEQYEVSLQGKVQALRELFQPYWSSVLTVFRSPPVHHRLRAKFGIGRVITRNEGSQXXXXXXXXXXXXXXLEKISEGTAANTMTSSKLCYLMWGYDQR-PVAISSFPLASRRINDLMPRLLQALEAGPVVLRRGLRAVNFLDTLAGDTLISLMYQVPLIEGGKEGGQGGFWAEAAGIWLR----DGLGCQVVGRSKGRVVAVDRAYVTEAFHLFPCSSSSSSPCFSSFYLGNNCEGKGDKARKAEELVSFFPPPSSSPHPSPCSHIPVPTILRYKQWEGAFSNPNGQMAMHTIRWLCARADAILLAADEAKTEADEEGGNRAHEGLGGCGESKTGSDKXXXXXXXXXXXXXXXXXXXRQNCNHHSTKSTCDLLELYCGGGNHTVALSRKFQRVVGVEIDKRLVAVARENLVLNGIENTTIIQIPSEKFRVEELGKYRRKEREKERKAAAAVVPGEVEGMKVGDCGNSKEVEXXXXXXXXXXXXXXXXXXXFNFGVVLVDPPRVGLDRHTRKLISQYEHILYISCGPHSMLRDLQGEDEEKQERSTDKTYRSLAGFGLVETHDVVDMCVLDHFPGTMHIEAAVHLRRR 619
            D + ++Y+  L  KVQ L ++F PY +  L VF SP  H+R+RA+F +      +EG +                               + Y+M+  + R    +  FP ASR INDLMP L++A++ G   LRR L  V+FL TL+G+ L+SL+Y   L E          W E A    +    +G    ++GR++   + +DR YV E                       +  GK                               P I  Y+Q E +F+ PNG++A   + W          A D                    C +   G                                   DLLELYCG GN ++AL++ F+RV+  E+ K  V  A+ N+  N IEN  II++ +E+F     GK     RE  R   A V                                       +N   + VDPPR G+D  T K++  YE ILYISC P ++L +L+                      L ETH +    + D FP T H+EA V L R+
Sbjct:    5 DVNPQRYQAQLAEKVQRLTDMFAPYQAPELEVFESPEQHYRMRAEFRVW-----HEGDE-------------------------------MYYIMFNQETREKYRVDQFPAASRLINDLMPLLIEAMQ-GNESLRRKLFQVDFLSTLSGEILVSLLYHRQLDEA---------WIENAKTLKQRLNDEGFNLNLIGRARKMKIVLDRDYVIEKL---------------------DVNGK-------------------------------PYI--YQQVENSFTQPNGKVAEKMLAW----------AVD--------------------CTQESQG-----------------------------------DLLELYCGNGNFSLALAQNFERVLATELAKPSVESAQYNIAANKIENVQIIRMSAEEFTEAMEGK-----REFRRLKDAGV-----------------------------------DLKSYNCNTIFVDPPRAGMDIDTCKMVQGYERILYISCNPETLLENLE---------------------ILSETHTITRFALFDQFPYTHHMEAGVMLERK 368