NO12G01850, NO12G01850 (gene) Nannochloropsis oceanica

Overview
NameNO12G01850
Unique NameNO12G01850
Typegene
OrganismNannochloropsis oceanica (N. oceanica IMET1)
Sequence length560
Alignment locationchr12:534170..534729 +

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Properties
Property NameValue
DescriptionCytochrome b5 reductase 4
Mutants
Expression

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
chr12genomechr12:534170..534729 +
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
PRJNA7699582024-08-13
GSE1786722023-12-15
PRJNA9336932023-10-26
PRJNA9434492023-07-19
GSE1499042020-10-10
NoIMET1_WT_HS2020-09-29
GSE1396152020-03-11
PRJNA2413822020-03-11
BLASTP analysis of N. oceanica IMET1 genes2019-07-11
InterPro analysis for N. oceanica IMET1 genes2019-07-11
PRJNA1821802019-04-25
Gene prediction for N. oceanica IMET12017-10-24
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR036400Cyt_B5-like_heme/steroid_sf
IPR001199Cyt_B5-like_heme/steroid-bd
Homology
BLAST of NO12G01850 vs. NCBI_GenBank
Match: XP_005856099.1 (hypothetical protein NGA_0331900 [Nannochloropsis gaditana CCMP526] >EKU20235.1 hypothetical protein NGA_0331900 [Nannochloropsis gaditana CCMP526] >EWM25821.1 cytochrome b5 reductase 4 [Nannochloropsis gaditana])

HSP 1 Score: 149.1 bits (375), Expect = 8.300e-33
Identity = 66/78 (84.62%), Postives = 74/78 (94.87%), Query Frame = 0
Query:    1 MATGTPTNKTALKKGFGLMDWVKLTTSTSDLSGMLRMGGMRGYRMDEVAKHTSAGDAWAVFGGKVYNLTPYLPYHPGG 79
            MATGTP+NKTAL+KGFGLMDWVKLTTST DLSG+LRMGG+RGYRM+EVA+H ++ DAWAVF GKVYNLTPYLPYHPGG
Sbjct:    1 MATGTPSNKTALRKGFGLMDWVKLTTSTRDLSGVLRMGGLRGYRMEEVARHRTSNDAWAVFAGKVYNLTPYLPYHPGG 78          
BLAST of NO12G01850 vs. NCBI_GenBank
Match: XP_013148264.1 (PREDICTED: cytochrome b5 reductase 4-like [Papilio polytes])

HSP 1 Score: 85.1 bits (209), Expect = 1.500e-13
Identity = 40/77 (51.95%), Postives = 49/77 (63.64%), Query Frame = 0
Query:    2 ATGTPTNKTALKKGFGLMDWVKLTTSTSDLSGMLRMGGMRGYRMDEVAKHTSAGDAWAVFGGKVYNLTPYLPYHPGG 79
            ATG P NK AL+ G  LMDW++L  S  DL+G+   G MR     E+A H +  DAW    G+VYN+T YLPYHPGG
Sbjct:  115 ATGNPRNKCALQPGHSLMDWIRLGNSGKDLTGV--GGRMRPVHPSELAAHNTKNDAWLAIRGRVYNITHYLPYHPGG 189          
BLAST of NO12G01850 vs. NCBI_GenBank
Match: XP_014360030.1 (PREDICTED: cytochrome b5 reductase 4-like [Papilio machaon])

HSP 1 Score: 85.1 bits (209), Expect = 1.500e-13
Identity = 40/77 (51.95%), Postives = 49/77 (63.64%), Query Frame = 0
Query:    2 ATGTPTNKTALKKGFGLMDWVKLTTSTSDLSGMLRMGGMRGYRMDEVAKHTSAGDAWAVFGGKVYNLTPYLPYHPGG 79
            ATG P NK AL+ G  LMDW++L  S  DL+G+   G MR     E+A H +  DAW    G+VYN+T YLPYHPGG
Sbjct:  114 ATGNPRNKCALQPGHSLMDWIRLGNSGKDLTGV--GGRMRPVHPSELATHNTKNDAWLAIRGRVYNITHYLPYHPGG 188          
BLAST of NO12G01850 vs. NCBI_GenBank
Match: XP_021708928.1 (cytochrome b5 reductase 4 isoform X2 [Aedes aegypti] >XP_021708929.1 cytochrome b5 reductase 4 isoform X2 [Aedes aegypti] >XP_021708930.1 cytochrome b5 reductase 4 isoform X2 [Aedes aegypti] >XP_021708931.1 cytochrome b5 reductase 4 isoform X2 [Aedes aegypti])

HSP 1 Score: 84.7 bits (208), Expect = 1.900e-13
Identity = 40/78 (51.28%), Postives = 50/78 (64.10%), Query Frame = 0
Query:    2 ATGTPTNKTALKKGFGLMDWVKLTTSTSDLSGMLRMGGMRGYRMDEVAKHTSAGDAWAVFGGKVYNLTPYLPYHPGGL 80
            ATG P NKTALK G  LMDW++L  S +DL+G    G +R  +  E+A H   GD W    GKVYN+T YL +HPGG+
Sbjct:   42 ATGNPRNKTALKPGHSLMDWIRLGNSGADLAG--TGGRVRPVQHSELASHNKPGDMWMAIRGKVYNVTSYLDFHPGGV 117          
BLAST of NO12G01850 vs. NCBI_GenBank
Match: KZS14580.1 (NADH-cytochrome b5 reductase 1 [Daphnia magna])

HSP 1 Score: 84.3 bits (207), Expect = 2.500e-13
Identity = 39/77 (50.65%), Postives = 51/77 (66.23%), Query Frame = 0
Query:    2 ATGTPTNKTALKKGFGLMDWVKLTTSTSDLSGMLRMGGMRGYRMDEVAKHTSAGDAWAVFGGKVYNLTPYLPYHPGG 79
            ATG P NKTALK G  LMDW+KLT S+++LSG      +R   +DE+A+H +  DAW    G VYN++ Y  +HPGG
Sbjct:   11 ATGNPRNKTALKPGRSLMDWIKLTNSSTNLSG--NGNRLRDISLDELAQHKNKNDAWLAIRGMVYNVSSYFEFHPGG 85          
BLAST of NO12G01850 vs. NCBI_GenBank
Match: EFX79716.1 (hypothetical protein DAPPUDRAFT_52230 [Daphnia pulex])

HSP 1 Score: 84.0 bits (206), Expect = 3.300e-13
Identity = 40/76 (52.63%), Postives = 49/76 (64.47%), Query Frame = 0
Query:    3 TGTPTNKTALKKGFGLMDWVKLTTSTSDLSGMLRMGGMRGYRMDEVAKHTSAGDAWAVFGGKVYNLTPYLPYHPGG 79
            TG P NKTALK G  LMDW+KLT S++DLSG      +R    +E+A+H S  DAW    G VYN+T Y  +HPGG
Sbjct:   10 TGNPRNKTALKPGRSLMDWIKLTNSSTDLSG--TGNRLRDISPNELAQHNSKNDAWLAIRGMVYNVTSYFEFHPGG 83          
BLAST of NO12G01850 vs. NCBI_GenBank
Match: XP_022916780.1 (cytochrome b5 reductase 4 isoform X1 [Onthophagus taurus])

HSP 1 Score: 84.0 bits (206), Expect = 3.300e-13
Identity = 36/78 (46.15%), Postives = 51/78 (65.38%), Query Frame = 0
Query:    2 ATGTPTNKTALKKGFGLMDWVKLTTSTSDLSGMLRMGGMRGYRMDEVAKHTSAGDAWAVFGGKVYNLTPYLPYHPGGL 80
            ATG P NK AL+ G  LMDW++L +S  DL+G+    G     + E+A+H++  D W    G+VYN+T YLP+HPGG+
Sbjct:   51 ATGNPRNKCALQPGHSLMDWIRLGSSGKDLTGVGSKAGQLTITLKELAQHSTETDVWLALRGRVYNVTHYLPFHPGGI 128          
BLAST of NO12G01850 vs. NCBI_GenBank
Match: EFJ04902.1 (hypothetical protein SELMODRAFT_138045 [Selaginella moellendorffii] >EFJ11012.1 hypothetical protein SELMODRAFT_127066 [Selaginella moellendorffii])

HSP 1 Score: 83.6 bits (205), Expect = 4.300e-13
Identity = 37/72 (51.39%), Postives = 46/72 (63.89%), Query Frame = 0
Query:    8 NKTALKKGFGLMDWVKLTTSTSDLSGMLRMGGMRGYRMDEVAKHTSAGDAWAVFGGKVYNLTPYLPYHPGGL 80
            NK   +KGF  MDW+KLT S  DL+G+      R   M+EV +H    DAW V  G+VYN+TPYL +HPGGL
Sbjct:    8 NKVPFEKGFSQMDWLKLTQSHPDLAGLKGRSNRRMIAMEEVKQHRKQDDAWTVLRGRVYNITPYLNFHPGGL 79          
BLAST of NO12G01850 vs. NCBI_GenBank
Match: XP_005844825.1 (hypothetical protein CHLNCDRAFT_36685 [Chlorella variabilis] >EFN52723.1 hypothetical protein CHLNCDRAFT_36685 [Chlorella variabilis])

HSP 1 Score: 83.6 bits (205), Expect = 4.300e-13
Identity = 37/76 (48.68%), Postives = 49/76 (64.47%), Query Frame = 0
Query:    6 PTNKTALKKGFGLMDWVKLTTSTSDLSGMLRMGGMRGYRMDEVAKHTSAGDAWAVFGGKVYNLTPYLPYHPGGLAL 82
            P  K   +KGF  MDWV+LT +  DL+G+      RG  M+EVA+H +  DAW V  G+VYN+T YL +HPGG+ L
Sbjct:   21 PRKKVPFEKGFSQMDWVRLTQTHPDLAGLGGQRPRRGITMEEVARHNTRDDAWTVLRGRVYNITHYLGFHPGGVPL 96          
BLAST of NO12G01850 vs. NCBI_GenBank
Match: XP_018323908.1 (PREDICTED: cytochrome b5 reductase 4 isoform X1 [Agrilus planipennis])

HSP 1 Score: 83.6 bits (205), Expect = 4.300e-13
Identity = 37/77 (48.05%), Postives = 47/77 (61.04%), Query Frame = 0
Query:    2 ATGTPTNKTALKKGFGLMDWVKLTTSTSDLSGMLRMGGMRGYRMDEVAKHTSAGDAWAVFGGKVYNLTPYLPYHPGG 79
            ATG P NK AL  G  LMDW++L  S +DL+G+    G       E+A+H    DAW    GKV+N+T Y+PYHPGG
Sbjct:   63 ATGNPRNKHALSPGHSLMDWIRLGNSGTDLTGVGSQAGRLSVTHKELARHNKETDAWLAIRGKVFNITHYIPYHPGG 139          
The following BLAST results are available for this feature:
BLAST of NO12G01850 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes)
Total hits: 20
Match NameE-valueIdentityDescription
XP_005856099.18.300e-3384.62hypothetical protein NGA_0331900 [Nannochloropsis ... [more]
XP_013148264.11.500e-1351.95PREDICTED: cytochrome b5 reductase 4-like [Papilio... [more]
XP_014360030.11.500e-1351.95PREDICTED: cytochrome b5 reductase 4-like [Papilio... [more]
XP_021708928.11.900e-1351.28cytochrome b5 reductase 4 isoform X2 [Aedes aegypt... [more]
KZS14580.12.500e-1350.65NADH-cytochrome b5 reductase 1 [Daphnia magna][more]
EFX79716.13.300e-1352.63hypothetical protein DAPPUDRAFT_52230 [Daphnia pul... [more]
XP_022916780.13.300e-1346.15cytochrome b5 reductase 4 isoform X1 [Onthophagus ... [more]
EFJ04902.14.300e-1351.39hypothetical protein SELMODRAFT_138045 [Selaginell... [more]
XP_005844825.14.300e-1348.68hypothetical protein CHLNCDRAFT_36685 [Chlorella v... [more]
XP_018323908.14.300e-1348.05PREDICTED: cytochrome b5 reductase 4 isoform X1 [A... [more]

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Relationships

This gene is member of the following syntenic_region feature(s):

Feature NameUnique NameSpeciesType
nonsL124nonsL124Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ncniR014ncniR014Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ngnoR006ngnoR006Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region


This gene is orthologous to the following gene feature(s):

Feature NameUnique NameSpeciesType
NSK010107NSK010107Nannochloropsis salina (N. salina CCMP1776)gene


The following polypeptide feature(s) derives from this gene:

Feature NameUnique NameSpeciesType
NO12G01850.1NO12G01850.1-proteinNannochloropsis oceanica (N. oceanica IMET1)polypeptide


The following gene feature(s) are orthologous to this gene:

Feature NameUnique NameSpeciesType
jgi.p|Nanoce1779_2|592199gene_5342Nannochloropsis oceanica (N. oceanica CCMP1779)gene
Naga_100021g2gene4666Nannochloropsis gaditana (N. gaditana B-31)gene


The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
NO12G01850.1NO12G01850.1Nannochloropsis oceanica (N. oceanica IMET1)mRNA


Sequences
The following sequences are available for this feature:

gene sequence

>NO12G01850 ID=NO12G01850|Name=NO12G01850|organism=Nannochloropsis oceanica|type=gene|length=560bp
ATGGCGACGGGTACGCCCACCAATAAGACTGCCCTGAAAAAGGGCTTTGG
GCTCATGgtacgtgggaccacacgagctcacggccctgtggtgcgaatgc
cacgtactcactcctttcacgcccccgtgtctgcatcatccactgcactg
tagGATTGGGTCAAGCTTACGACTTCCACCTCGGACTTGTCCGGAATGCT
GCGCATGGGGGGCATGCGAGGGTATCGAATGGACGAAGTTGCCAAGCACA
CAAGTGCAGGGGACGCTTGGGCTGTGTTTGGTGGCAAGGTTTACAACTTG
ACGCCTTACCTGCCTTATCACCCGGGGGGGCTGGCATTGTGGTCAAGGCG
GCGGGTACAGACTGCACGGCGTTATTTAATAAGCACCATCCGTGGGTCAA
CTTTGAGGGTTTGGCGGGGgtacgtttatttttgtttttgggaggggaga
gtaggagggcgggagggaggttcggattatgagagtaaatgtccttctct
gtgtttttcttggtatttataattatgcttacggcgctattatcattatg
cagAAATTGA
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protein sequence of NO12G01850.1

>NO12G01850.1-protein ID=NO12G01850.1-protein|Name=NO12G01850.1|organism=Nannochloropsis oceanica|type=polypeptide|length=110bp
MATGTPTNKTALKKGFGLMDWVKLTTSTSDLSGMLRMGGMRGYRMDEVAK
HTSAGDAWAVFGGKVYNLTPYLPYHPGGLALWSRRRVQTARRYLISTIRG
STLRVWRGN*
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Synonyms
Publications