NO06G01310, NO06G01310 (gene) Nannochloropsis oceanica

Overview
NameNO06G01310
Unique NameNO06G01310
Typegene
OrganismNannochloropsis oceanica (N. oceanica IMET1)
Sequence length778
Alignment locationchr6:425625..426402 +

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Properties
Property NameValue
DescriptionAdenosine trna methylthiotransferase
Mutants
Expression

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
chr6genomechr6:425625..426402 +
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
PRJNA7699582024-08-13
GSE1786722023-12-15
PRJNA9336932023-10-26
PRJNA9434492023-07-19
GSE1499042020-10-10
NoIMET1_WT_HS2020-09-29
GSE1396152020-03-11
PRJNA2413822020-03-11
BLASTP analysis of N. oceanica IMET1 genes2019-07-11
InterPro analysis for N. oceanica IMET1 genes2019-07-11
GO annotation for IMET1v2 genes2019-07-10
PRJNA1821802019-04-25
Gene prediction for N. oceanica IMET12017-10-24
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:00515394 iron, 4 sulfur cluster binding
GO:0016740transferase activity
GO:0005488binding
Vocabulary: INTERPRO
TermDefinition
IPR013848Methylthiotransferase_N
Vocabulary: Biological Process
TermDefinition
GO:0006464cellular protein modification process
GO:0006139nucleobase-containing compound metabolic process
Homology
BLAST of NO06G01310 vs. NCBI_GenBank
Match: EWM22418.1 (adenosine trna methylthiotransferase [Nannochloropsis gaditana])

HSP 1 Score: 214.5 bits (545), Expect = 2.000e-52
Identity = 107/124 (86.29%), Postives = 116/124 (93.55%), Query Frame = 0
Query:    1 MFDLSYTSKPQGRVQGRGLMPTPLKQLMAKEGADADAALTYAVESMGCAMNTADSERMAGQLMALGFKEASTPQEVTKADVVVVNTCSIRDHAEQKVYSYLGPYINRKKRGDNLAIVVAGCVAQ 125
            MFDLSYTSKPQGRVQGRGL+PTPLK+LMAK+    DAAL YAVE+MGCAMNTADSERMAGQL ALGF+EA++ +EV KADVVVVNTCSIRDHAEQKVYSYLGPY+NRKKRGDNLAIVVAGCVAQ
Sbjct:  209 MFDLSYTSKPQGRVQGRGLLPTPLKELMAKD--KDDAALVYAVETMGCAMNTADSERMAGQLSALGFREAASSEEVAKADVVVVNTCSIRDHAEQKVYSYLGPYVNRKKRGDNLAIVVAGCVAQ 330          
BLAST of NO06G01310 vs. NCBI_GenBank
Match: GAX11752.1 (tRNA-2-methylthio-N6-dimethylallyladenosine synthase [Fistulifera solaris])

HSP 1 Score: 117.1 bits (292), Expect = 4.400e-23
Identity = 58/94 (61.70%), Postives = 70/94 (74.47%), Query Frame = 0
Query:   31 EGADADAALTYAVESMGCAMNTADSERMAGQLMALGFKEASTPQEVTKADVVVVNTCSIRDHAEQKVYSYLGPYINRKKRGDNLAIVVAGCVAQ 125
            E    D  LTY +E+MGC MNTAD+ERM GQL  LG + +   +E    DV+V+NTCSIRDHAEQKVYSYLGPY  RK+ G+++ IVVAGCVAQ
Sbjct:  207 EALRRDTDLTYKMETMGCQMNTADAERMEGQLQDLGLRPS---EEGQNPDVIVLNTCSIRDHAEQKVYSYLGPYAKRKRNGEDVTIVVAGCVAQ 297          
BLAST of NO06G01310 vs. NCBI_GenBank
Match: GAX20818.1 (tRNA-2-methylthio-N6-dimethylallyladenosine synthase [Fistulifera solaris])

HSP 1 Score: 116.3 bits (290), Expect = 7.600e-23
Identity = 58/94 (61.70%), Postives = 70/94 (74.47%), Query Frame = 0
Query:   31 EGADADAALTYAVESMGCAMNTADSERMAGQLMALGFKEASTPQEVTKADVVVVNTCSIRDHAEQKVYSYLGPYINRKKRGDNLAIVVAGCVAQ 125
            E    D  LTY +E+MGC MNTADSERM GQL  LG + +   +E    DV+V+NTCSIRDHAEQKVYSYLGP+  RK+ G+++ IVVAGCVAQ
Sbjct:  202 EALRRDTDLTYKMETMGCQMNTADSERMEGQLQDLGLRPS---EEGQNPDVIVLNTCSIRDHAEQKVYSYLGPHAKRKRNGEDVTIVVAGCVAQ 292          
BLAST of NO06G01310 vs. NCBI_GenBank
Match: EJK59086.1 (hypothetical protein THAOC_20733 [Thalassiosira oceanica])

HSP 1 Score: 115.2 bits (287), Expect = 1.700e-22
Identity = 59/109 (54.13%), Postives = 73/109 (66.97%), Query Frame = 0
Query:   31 EGADADAALTYAVESMGCAMNTADSERMAGQLMALGFKEASTPQEVTKA---------------DVVVVNTCSIRDHAEQKVYSYLGPYINRKKRGDNLAIVVAGCVAQ 125
            E    D  LTY +E+MGC MN ADSERM GQLM+LG +     + VTK+               DV+V+NTCSIRDHAEQKVYSYLGP+  RK+ G+++ I+VAGCVAQ
Sbjct:  296 EDGVVDVDLTYKIETMGCQMNQADSERMEGQLMSLGIRPLLEDETVTKSSGLFEETVKESKRKPDVIVLNTCSIRDHAEQKVYSYLGPHAKRKRDGEDVTIIVAGCVAQ 404          
BLAST of NO06G01310 vs. NCBI_GenBank
Match: XP_009040921.1 (hypothetical protein AURANDRAFT_32629 [Aureococcus anophagefferens] >EGB04364.1 hypothetical protein AURANDRAFT_32629 [Aureococcus anophagefferens])

HSP 1 Score: 112.8 bits (281), Expect = 8.400e-22
Identity = 63/124 (50.81%), Postives = 80/124 (64.52%), Query Frame = 0
Query:    1 MFDLSYTSKPQGRVQGRGLMPTPLKQLMAKEGADADAALTYAVESMGCAMNTADSERMAGQLMALGFKEASTPQEVTKADVVVVNTCSIRDHAEQKVYSYLGPYINRKKRGDNLAIVVAGCVAQ 125
            M DL   +KP+ +V+G            A + A A A  TYA+ +MGC MN ADSER+ G L  LG  EA  P+    A+VVV+NTCSIR+HAE KVYSY+GP   RK+ G+++ IVVAGCVAQ
Sbjct:    1 MHDLDLATKPRKKVRG-----------AATDLAGACAGKTYAMVTMGCQMNAADSERLEGSLRQLGLVEAPDPK---LANVVVLNTCSIREHAETKVYSYVGPQAKRKRNGEDVTIVVAGCVAQ 110          
BLAST of NO06G01310 vs. NCBI_GenBank
Match: XP_005794442.1 (hypothetical protein EMIHUDRAFT_308277 [Emiliania huxleyi CCMP1516] >EOD42013.1 hypothetical protein EMIHUDRAFT_308277 [Emiliania huxleyi CCMP1516])

HSP 1 Score: 111.3 bits (277), Expect = 2.400e-21
Identity = 56/82 (68.29%), Postives = 67/82 (81.71%), Query Frame = 0
Query:   43 VESMGCAMNTADSERMAGQLMALGFKEASTPQEVTKADVVVVNTCSIRDHAEQKVYSYLGPYINRKKRGDNLAIVVAGCVAQ 125
            ++SMGC MNTAD+ERM GQL +LGF  A   +E T A VVV+NTCSIR+HAE KVYSYLGP+  RK+ G++LAIVVAGCVAQ
Sbjct:    1 MDSMGCQMNTADAERMEGQLRSLGFARA---EEGTPAQVVVLNTCSIREHAEAKVYSYLGPHAARKRAGEDLAIVVAGCVAQ 79          
BLAST of NO06G01310 vs. NCBI_GenBank
Match: XP_005826573.1 (hypothetical protein GUITHDRAFT_96633 [Guillardia theta CCMP2712] >EKX39593.1 hypothetical protein GUITHDRAFT_96633 [Guillardia theta CCMP2712])

HSP 1 Score: 110.5 bits (275), Expect = 4.200e-21
Identity = 52/79 (65.82%), Postives = 65/79 (82.28%), Query Frame = 0
Query:   46 MGCAMNTADSERMAGQLMALGFKEASTPQEVTKADVVVVNTCSIRDHAEQKVYSYLGPYINRKKRGDNLAIVVAGCVAQ 125
            MGC MN+ADSERMAGQL A+G  +   P   +KA+VV+ NTCSIRDHAEQKVYSY+GP  +RK+ G+N+A++VAGCVAQ
Sbjct:    1 MGCQMNSADSERMAGQLEAMGMAKVEDP---SKANVVIYNTCSIRDHAEQKVYSYIGPQADRKRNGENVALIVAGCVAQ 76          
BLAST of NO06G01310 vs. NCBI_GenBank
Match: CBJ34118.1 (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 110.2 bits (274), Expect = 5.400e-21
Identity = 53/82 (64.63%), Postives = 67/82 (81.71%), Query Frame = 0
Query:   43 VESMGCAMNTADSERMAGQLMALGFKEASTPQEVTKADVVVVNTCSIRDHAEQKVYSYLGPYINRKKRGDNLAIVVAGCVAQ 125
            +E+MGC MN ADSERM GQ+  LG ++    ++ T+A V V+NTCSIRDHAEQKVYSY+GPY  RK++G+N+AIVVAGCVAQ
Sbjct:    1 METMGCQMNVADSERMEGQMADLGIQKT---EDKTEASVFVLNTCSIRDHAEQKVYSYVGPYAIRKQKGENIAIVVAGCVAQ 79          
BLAST of NO06G01310 vs. NCBI_GenBank
Match: XP_002178193.1 (predicted protein [Phaeodactylum tricornutum CCAP 1055/1] >EEC49858.1 predicted protein [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 109.4 bits (272), Expect = 9.200e-21
Identity = 52/87 (59.77%), Postives = 66/87 (75.86%), Query Frame = 0
Query:   39 LTYAVESMGCAMNTADSERMAGQLMALGFKEASTPQEVTK-ADVVVVNTCSIRDHAEQKVYSYLGPYINRKKRGDNLAIVVAGCVAQ 125
            LTY +E+MGC MN ADSER+ GQL  LG +      +  K  DVV++NTCSIRDHAEQKVYSY+GP+  RK+ G+++ I+VAGCVAQ
Sbjct:   13 LTYKLETMGCQMNMADSERIEGQLQGLGIRPLDPDVDKNKQPDVVILNTCSIRDHAEQKVYSYIGPHAKRKRDGEDVTIIVAGCVAQ 99          
BLAST of NO06G01310 vs. NCBI_GenBank
Match: XP_002297013.1 (predicted protein [Thalassiosira pseudonana CCMP1335] >EED86741.1 predicted protein [Thalassiosira pseudonana CCMP1335])

HSP 1 Score: 105.9 bits (263), Expect = 1.000e-19
Identity = 51/79 (64.56%), Postives = 62/79 (78.48%), Query Frame = 0
Query:   46 MGCAMNTADSERMAGQLMALGFKEASTPQEVTKADVVVVNTCSIRDHAEQKVYSYLGPYINRKKRGDNLAIVVAGCVAQ 125
            MGC MN ADSER+ GQLM+LG +      E  K DV+V+NTCSIRDHAEQKVYSYLGP+  RK+ G+++ I+VAGCVAQ
Sbjct:    1 MGCQMNLADSERIEGQLMSLGIRPLG---EDEKPDVIVLNTCSIRDHAEQKVYSYLGPHAKRKRAGEDITIIVAGCVAQ 76          
The following BLAST results are available for this feature:
BLAST of NO06G01310 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes)
Total hits: 20
Match NameE-valueIdentityDescription
EWM22418.12.000e-5286.29adenosine trna methylthiotransferase [Nannochlorop... [more]
GAX11752.14.400e-2361.70tRNA-2-methylthio-N6-dimethylallyladenosine syntha... [more]
GAX20818.17.600e-2361.70tRNA-2-methylthio-N6-dimethylallyladenosine syntha... [more]
EJK59086.11.700e-2254.13hypothetical protein THAOC_20733 [Thalassiosira oc... [more]
XP_009040921.18.400e-2250.81hypothetical protein AURANDRAFT_32629 [Aureococcus... [more]
XP_005794442.12.400e-2168.29hypothetical protein EMIHUDRAFT_308277 [Emiliania ... [more]
XP_005826573.14.200e-2165.82hypothetical protein GUITHDRAFT_96633 [Guillardia ... [more]
CBJ34118.15.400e-2164.63conserved unknown protein [Ectocarpus siliculosus][more]
XP_002178193.19.200e-2159.77predicted protein [Phaeodactylum tricornutum CCAP ... [more]
XP_002297013.11.000e-1964.56predicted protein [Thalassiosira pseudonana CCMP13... [more]

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Relationships

This gene is member of the following syntenic_region feature(s):

Feature NameUnique NameSpeciesType
nonsL091nonsL091Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
nonsL089nonsL089Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ngnoR147ngnoR147Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ngnoR145ngnoR145Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region


The following polypeptide feature(s) derives from this gene:

Feature NameUnique NameSpeciesType
NO06G01310.1NO06G01310.1-proteinNannochloropsis oceanica (N. oceanica IMET1)polypeptide


The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
NO06G01310.1NO06G01310.1Nannochloropsis oceanica (N. oceanica IMET1)mRNA


Sequences
The following sequences are available for this feature:

gene sequence

>NO06G01310 ID=NO06G01310|Name=NO06G01310|organism=Nannochloropsis oceanica|type=gene|length=778bp
TAATAGAGGATGTCATGTTCGACCTTAGCTACACCTCCAAGCCTCAGGGT
CGAGTGCAGGGCCGGGGTTTGATGCCGACGCCTCTCAAACAGCTCATGGC
TAAGGAGGGGGCCGATGCGGACGCCGCGCTGACGTACGCAGTAGAATCAA
TGGGCTGTGCGATGAACACGGCAGACTCGGAGCGTATGGCAGGACAATTG
ATGGCGCTGGGCTTCAAGGAGGCCTCCACGCCACAGGAGGTCACAAAGGC
CGATGTGGTGGTTGTTAATACTTGTTCCATCCGTGATCACGCCGAGCAAA
AAGTCTACAGCTATCTTGGCCCGTACATCAACCGGAAGAAGCGCGGCGAC
AACCTGGCCATTGTCGTGGCAGGCTGTGTGGCGCAGCATCCTTGTCCACG
CACACAAGCCCTTATTCAGATTCACAATTCCTGAGCATCCTCACCAAAGG
ACCCATCGTGTCTTCTCTACATCACTCACTCTGTTAGGGTTAGCACGCTT
TTTGTCTTGATCTCTATTTGCCGCCGAGTCCCATCCTTTGAGCTCTACTT
CCCCTTGCATTCCTTTCCTTCTTCCCTCCTTCCCTCCCTTCCCTCAAGCC
CAGCGCAGGCAAGGCGGCAGGAATTGCAACCCCAAGTAGTCCCACACCTC
CTCCTTCCCCTTTTCAACCTACAGAGGCACAAACAGGTAAACCTCTCCTA
TGTGCGTCAGGGAAGAGTTCGGAAACGCTCGTAAAGCCTCCCAGCAATTC
CTCCTCCCTCTCTCCCCTTCCTCCCCCC
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protein sequence of NO06G01310.1

>NO06G01310.1-protein ID=NO06G01310.1-protein|Name=NO06G01310.1|organism=Nannochloropsis oceanica|type=polypeptide|length=140bp
MFDLSYTSKPQGRVQGRGLMPTPLKQLMAKEGADADAALTYAVESMGCAM
NTADSERMAGQLMALGFKEASTPQEVTKADVVVVNTCSIRDHAEQKVYSY
LGPYINRKKRGDNLAIVVAGCVAQHPCPRTQALIQIHNS*
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Synonyms
Publications