NO01G02120, NO01G02120 (gene) Nannochloropsis oceanica

Overview
NameNO01G02120
Unique NameNO01G02120
Typegene
OrganismNannochloropsis oceanica (N. oceanica IMET1)
Sequence length1286
Alignment locationchr1:539801..541086 -

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Properties
Property NameValue
DescriptionCobalamin (Vitamin B12) biosynthesis CbiX
Mutants
Expression

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
chr1genomechr1:539801..541086 -
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
GSE1786722023-12-15
PRJNA9336932023-10-26
PRJNA9434492023-07-19
GSE1499042020-10-10
NoIMET1_WT_HS2020-09-29
GSE1396152020-03-11
PRJNA2413822020-03-11
BLASTP analysis of N. oceanica IMET1 genes2019-07-11
InterPro analysis for N. oceanica IMET1 genes2019-07-11
GO annotation for IMET1v2 genes2019-07-10
PRJNA1821802019-04-25
Gene prediction for N. oceanica IMET12017-10-24
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0009236cobalamin biosynthetic process
GO:0009987cellular process
GO:0009058biosynthetic process
Vocabulary: Molecular Function
TermDefinition
GO:0016852sirohydrochlorin cobaltochelatase activity
GO:0003824catalytic activity
Vocabulary: INTERPRO
TermDefinition
IPR002762Cbl_biosynth_CbiX
Homology
[Close]
BLAST of NO01G02120 vs. NCBI_GenBank
Match: EWM29197.1 (Cobalamin (vitamin B12) biosynthesis CbiX [Nannochloropsis gaditana])

HSP 1 Score: 260.4 bits (664), Expect = 6.200e-66
Identity = 151/203 (74.38%), Postives = 165/203 (81.28%), Query Frame = 0 
Query:   68 MQFPTLSDTLRVSKGREVVIET--TATEHLEEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGVIVVDHGSRRAEANNALAGVVNDFQALSGYELVEPAHMELAEPSIATAFGRCVERGATFIICHPFFLSRGRHVAEDIPTLLEEAASIFPGVEWALSQPLGSQAEIPRLMQMAVEDCVVEQNIKSEDHADGSPLPEFNI 269
            MQ P+ S    V K R  ++ET  TAT+HLE+            XXXXXXXXXXXXXXXX   VGVIVVDHGSRR EAN AL  V ++F+ALSGYELVEPAHMEL EPSIATAFGRCVERGATFIICHPFFLSRGRHVAEDIP LLEEAASIFPGVEWALSQPLG QA+IPRLMQ AVE+C+ E+NI+SEDHADG P+PEFNI
Sbjct:   55 MQSPSRSMNSWVPKDRRNMLETLSTATQHLEDLGIEEDAHGFQEXXXXXXXXXXXXXXXXEYKVGVIVVDHGSRRKEANEALESVASEFKALSGYELVEPAHMELCEPSIATAFGRCVERGATFIICHPFFLSRGRHVAEDIPNLLEEAASIFPGVEWALSQPLGLQADIPRLMQQAVEECIAEKNIQSEDHADGLPMPEFNI 257
[Close]
BLAST of NO01G02120 vs. NCBI_GenBank
Match: CBJ30308.1 (sirohydrochlorin ferrochelatase, putative chloroplast precursor [Ectocarpus siliculosus])

HSP 1 Score: 137.9 bits (346), Expect = 4.700e-29
Identity = 68/119 (57.14%), Postives = 81/119 (68.07%), Query Frame = 0 
Query:  129 VGVIVVDHGSRRAEANNALAGVVNDFQALSGYELVEPAHMELAEPSIATAFGRCVERGATFIICHPFFLSRGRHVAEDIPTLLEEAASIFPGVEWALSQPLGSQAEIPRLMQMAVEDCV 248
            VGVIV DHGSRR  AN  L  V   +++ SG+E+VE AHMELAEPSI  AF RCV  GA  ++ HPFFLS GRHV  DIP L+  AA   PGV W +S+PLG Q  +PR+M  AV + V
Sbjct:   87 VGVIVCDHGSRRENANTMLFEVAERYRSFSGFEIVEAAHMELAEPSIEEAFDRCVAAGAQKVVLHPFFLSPGRHVTSDIPALMVAAAKRHPGVGWVVSEPLGLQELMPRVMHAAVSESV 205
[Close]
BLAST of NO01G02120 vs. NCBI_GenBank
Match: WP_105359918.1 (cobalamin biosynthesis protein CbiX [Blastopirellula marina] >PQO27253.1 cobalamin biosynthesis protein CbiX [Blastopirellula marina] >PTL41399.1 cobalamin biosynthesis protein CbiX [Blastopirellula marina])

HSP 1 Score: 136.3 bits (342), Expect = 1.400e-28
Identity = 63/119 (52.94%), Postives = 88/119 (73.95%), Query Frame = 0 
Query:  129 VGVIVVDHGSRRAEANNALAGVVNDFQALSGYELVEPAHMELAEPSIATAFGRCVERGATFIICHPFFLSRGRHVAEDIPTLLEEAASIFPGVEWALSQPLGSQAEIPRLMQMAVEDCV 248
            +G+++VDHGSRRAE+N+ L  VV  F+  S YE+VE AHMELAEPSIA AF RCVE+GAT I+ HP+FL+ GRH  EDIP L +EA++   G+ + ++ PLG    + +++Q  +EDC+
Sbjct:   11 LGIVIVDHGSRRAESNDLLLEVVEQFRQASRYEIVEAAHMELAEPSIAAAFARCVEQGATQIVVHPYFLAPGRHWHEDIPRLAQEASTNHVGIHFTVTHPLGPHPLMLKIIQDRIEDCL 129
[Close]
BLAST of NO01G02120 vs. NCBI_GenBank
Match: WP_105336641.1 (hypothetical protein [Blastopirellula marina] >PQO44799.1 hypothetical protein C5Y93_17025 [Blastopirellula marina])

HSP 1 Score: 136.0 bits (341), Expect = 1.800e-28
Identity = 61/119 (51.26%), Postives = 87/119 (73.11%), Query Frame = 0 
Query:  129 VGVIVVDHGSRRAEANNALAGVVNDFQALSGYELVEPAHMELAEPSIATAFGRCVERGATFIICHPFFLSRGRHVAEDIPTLLEEAASIFPGVEWALSQPLGSQAEIPRLMQMAVEDCV 248
            +G+++VDHGSRRAE+N+ L  +V  F+  S Y +VEPAHMELAEPSIA AF +C ++GAT I+ HP+FLS GRH  EDIP L  EAA+  PG+ + ++ PLG    + ++++  +EDC+
Sbjct:   11 LGIVIVDHGSRRAESNDLLLEIVQQFREASDYAIVEPAHMELAEPSIADAFAKCAQQGATQIVVHPYFLSPGRHWHEDIPNLAAEAAANHPGLRYEVTAPLGPHPLMLKIIEERIEDCL 129
[Close]
BLAST of NO01G02120 vs. NCBI_GenBank
Match: WP_105351426.1 (cobalamin biosynthesis protein CbiX [Blastopirellula marina] >PQO36943.1 cobalamin biosynthesis protein CbiX [Blastopirellula marina])

HSP 1 Score: 135.2 bits (339), Expect = 3.000e-28
Identity = 63/119 (52.94%), Postives = 87/119 (73.11%), Query Frame = 0 
Query:  129 VGVIVVDHGSRRAEANNALAGVVNDFQALSGYELVEPAHMELAEPSIATAFGRCVERGATFIICHPFFLSRGRHVAEDIPTLLEEAASIFPGVEWALSQPLGSQAEIPRLMQMAVEDCV 248
            +G+I+VDHGSRRAE+N+ L  VV  F+  +GY++VEPAHMELA PSIA AF +C ++GAT I+ HP+FLS GRH  EDIP L +EAA+  PG+   ++ PLG    + ++MQ  +E C+
Sbjct:   11 LGIIIVDHGSRRAESNDLLLEVVKMFRQTAGYQIVEPAHMELAAPSIAEAFQKCADQGATQIVVHPYFLSPGRHWHEDIPRLAKEAAASHPGISHLVTAPLGLHPLMAKVMQERIETCL 129
[Close]
BLAST of NO01G02120 vs. NCBI_GenBank
Match: WP_105331933.1 (cobalamin biosynthesis protein CbiX [Blastopirellula marina] >PQO32921.1 cobalamin biosynthesis protein CbiX [Blastopirellula marina])

HSP 1 Score: 132.9 bits (333), Expect = 1.500e-27
Identity = 64/119 (53.78%), Postives = 85/119 (71.43%), Query Frame = 0 
Query:  129 VGVIVVDHGSRRAEANNALAGVVNDFQALSGYELVEPAHMELAEPSIATAFGRCVERGATFIICHPFFLSRGRHVAEDIPTLLEEAASIFPGVEWALSQPLGSQAEIPRLMQMAVEDCV 248
            +G+I+VDHGSRRAE+N+ L  VV  F+  S + +VEPAHMELAEPSIA AF RCV +GA  II HP+FLS GRH  EDIP L  EA++   GV + ++ PLG    + +++Q  +EDC+
Sbjct:   11 LGIIIVDHGSRRAESNDLLLEVVEQFRQTSDFGIVEPAHMELAEPSIAEAFARCVAQGANRIIVHPYFLSPGRHWHEDIPQLAREASANHAGVSFTVTHPLGPHPLMLKIIQDRIEDCL 129
[Close]
BLAST of NO01G02120 vs. NCBI_GenBank
Match: WP_081444667.1 (hypothetical protein [Blastopirellula marina])

HSP 1 Score: 128.3 bits (321), Expect = 3.700e-26
Identity = 65/132 (49.24%), Postives = 87/132 (65.91%), Query Frame = 0 
Query:  129 VGVIVVDHGSRRAEANNALAGVVNDFQALSGYELVEPAHMELAEPSIATAFGRCVERGATFIICHPFFLSRGRHVAEDIPTLLEEAASIFPGVEWALSQPLGSQAEIPRLMQMAVEDCVVEQNIKSEDHADG 261
            +G++VVDHGSRRA +N+ L  VV  F+  +G  +VE AHMELAEPSIA AF RCV +GA  +I HP+FLS GRH +EDIP L  +AA+  PGV   ++ PLG   ++  +M   +E C+     + ED A G
Sbjct:   49 IGIVVVDHGSRRAASNDLLLEVVQLFRQTTGRPIVEAAHMELAEPSIAAAFARCVAQGAGLVIVHPYFLSPGRHWSEDIPRLAAQAAAQHPGVRHMVTAPLGLHQKMAEIMAERIEVCLA--RCRGEDVACG 178
[Close]
BLAST of NO01G02120 vs. NCBI_GenBank
Match: EAQ82176.1 (cbiX protein [Blastopirellula marina DSM 3645])

HSP 1 Score: 128.3 bits (321), Expect = 3.700e-26
Identity = 65/132 (49.24%), Postives = 87/132 (65.91%), Query Frame = 0 
Query:  129 VGVIVVDHGSRRAEANNALAGVVNDFQALSGYELVEPAHMELAEPSIATAFGRCVERGATFIICHPFFLSRGRHVAEDIPTLLEEAASIFPGVEWALSQPLGSQAEIPRLMQMAVEDCVVEQNIKSEDHADG 261
            +G++VVDHGSRRA +N+ L  VV  F+  +G  +VE AHMELAEPSIA AF RCV +GA  +I HP+FLS GRH +EDIP L  +AA+  PGV   ++ PLG   ++  +M   +E C+     + ED A G
Sbjct:   11 IGIVVVDHGSRRAASNDLLLEVVQLFRQTTGRPIVEAAHMELAEPSIAAAFARCVAQGAGLVIVHPYFLSPGRHWSEDIPRLAAQAAAQHPGVRHMVTAPLGLHQKMAEIMAERIEVCLA--RCRGEDVACG 140
[Close]
BLAST of NO01G02120 vs. NCBI_GenBank
Match: PCJ59651.1 (cobalamin biosynthesis protein CbiX [Candidatus Hydrogenedentes bacterium])

HSP 1 Score: 127.1 bits (318), Expect = 8.200e-26
Identity = 58/116 (50.00%), Postives = 83/116 (71.55%), Query Frame = 0 
Query:  130 GVIVVDHGSRRAEANNALAGVVNDFQALSGYELVEPAHMELAEPSIATAFGRCVERGATFIICHPFFLSRGRHVAEDIPTLLEEAASIFPGVEWALSQPLGSQAEIPRLMQMAVED 246
            G+I++DHGS+R  AN  L  VV+ FQ  SG  +VEPAHMELA+P++A A+  CVE+GAT II HP+FL+ GRH   DIP L EEAA+++P + + +++PLG    I  ++Q  ++D
Sbjct:    7 GIILIDHGSKRTAANAQLLEVVSLFQKTSGARIVEPAHMELADPTLAEAYAACVEQGATEIIIHPYFLAPGRHSTSDIPRLAEEAAALYPLIPYRITKPLGVDTRICEVIQQRIDD 122
[Close]
BLAST of NO01G02120 vs. NCBI_GenBank
Match: KMZ64302.1 (Sirohydrochlorin cobaltochelatase [Zostera marina])

HSP 1 Score: 124.0 bits (310), Expect = 7.000e-25
Identity = 61/119 (51.26%), Postives = 79/119 (66.39%), Query Frame = 0 
Query:  130 GVIVVDHGSRRAEANNALAGVVNDFQALSGYELVEPAHMELAEPSIATAFGRCVERGATFIICHPFFLSRGRHVAEDIPTLLEEAASIFPGVEWALSQPLGSQAEIPRLMQMAVEDCVV 249
            GVI+VDHGSRR E+N  L   V  F A +GY +VEPAHMELAEPSI  AF  CV+RGAT II  PFFL  G+H ++DIP L  EAA   PG+ + ++ PLG    +  +M   ++ C++
Sbjct:   76 GVIIVDHGSRRKESNLMLNDFVGMFVARNGYRIVEPAHMELAEPSIKDAFDSCVQRGATRIIISPFFLLPGKHWSKDIPALAAEAAKAHPGISYVITAPLGLHELLVDVMNDRIQQCLM 194
The following BLAST results are available for this feature:
BLAST of NO01G02120 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes)
Total hits: 20
Match NameE-valueIdentityDescription
EWM29197.16.200e-6674.38Cobalamin (vitamin B12) biosynthesis CbiX [Nannoch... [more]
CBJ30308.14.700e-2957.14sirohydrochlorin ferrochelatase, putative chloropl... [more]
WP_105359918.11.400e-2852.94cobalamin biosynthesis protein CbiX [Blastopirellu... [more]
WP_105336641.11.800e-2851.26hypothetical protein [Blastopirellula marina] >PQO... [more]
WP_105351426.13.000e-2852.94cobalamin biosynthesis protein CbiX [Blastopirellu... [more]
WP_105331933.11.500e-2753.78cobalamin biosynthesis protein CbiX [Blastopirellu... [more]
WP_081444667.13.700e-2649.24hypothetical protein [Blastopirellula marina][more]
EAQ82176.13.700e-2649.24cbiX protein [Blastopirellula marina DSM 3645][more]
PCJ59651.18.200e-2650.00cobalamin biosynthesis protein CbiX [Candidatus Hy... [more]
KMZ64302.17.000e-2551.26Sirohydrochlorin cobaltochelatase [Zostera marina][more]

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Relationships

This gene is member of the following syntenic_region feature(s):

Feature NameUnique NameSpeciesType
nonsL006nonsL006Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ncniR047ncniR047Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ngnoR091ngnoR091Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region


This gene is orthologous to the following gene feature(s):

Feature NameUnique NameSpeciesType
NSK008129NSK008129Nannochloropsis salina (N. salina CCMP1776)gene


The following polypeptide feature(s) derives from this gene:

Feature NameUnique NameSpeciesType
NO01G02120.1NO01G02120.1-proteinNannochloropsis oceanica (N. oceanica IMET1)polypeptide


The following gene feature(s) are orthologous to this gene:

Feature NameUnique NameSpeciesType
jgi.p|Nanoce1779_2|576510gene_197Nannochloropsis oceanica (N. oceanica CCMP1779)gene
SirBgene1479Nannochloropsis gaditana (N. gaditana B-31)gene


The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
NO01G02120.1NO01G02120.1Nannochloropsis oceanica (N. oceanica IMET1)mRNA


Sequences
The following sequences are available for this feature:

gene sequence

>NO01G02120 ID=NO01G02120|Name=NO01G02120|organism=Nannochloropsis oceanica|type=gene|length=1286bp
ACAGGCAGCACTTTTACAAGTGCACAAGGAAGACGCGCTAGATCCGACTC
GTTGGCTGGTCGCTTTTTCGTTAGTGATCCCGCCTCTGGACAACTATTGC
TGGCTAGCCTAGAGCGCGCGCGCACGCCGATACCATCAACATCCACCCCA
GCCACTCGCCCGCATCACATCGCATCATGTGCAGGTACTCACATTCCTCG
CAGCTGCTCGTGGTCGCACTGCTTGGGACGGCGCAACTTGCCGTCCACGG
GTTCCTCTTACTCAACAAGTCACCACTGGTACAAAATCAGGACGCCCGGT
GGCGACCCAGTCCCAGCAGCGACAGCCTCAGCACCCGCCCCACCACTGCT
ACCATGCACACGGAAGGGCGGATTTGGATGCAGTTCCCAACCTTGTCTGA
CACGCTGCGCGTGAGCAAGGGCCGCGAGGTGGTCATCGAGACGACTGCCA
CCGAGCATTTGGAAGAGGCCGAGATCGAAGTGGACGCTCACGCCTTTCAA
CTGCAACAAGAGCAACAGAGGATACGGGAAAAGTCCCAGGCTCAAGCGAG
AGAGTACAAGGTGGGCGTGATCGTCGTGGATCATGGATCGCGCCGCGCTG
AGGCCAATAATGCGTTGGCCGGCGTTGTGAATGATTTCCAGGCCCTGAGT
GGATATGAGCTGGTGGAGCCCGCCCACATGGAGCTAGCAGAGCCCTCCAT
TGCCACAGCTTTTGGTCGATGCGTGGAACGTGGGGCCACATTTATTATTT
GTCATCCGTTTTTTTTGTCGAGGGGGCGGCATGTGGCGGAAGATATCCCA
ACGTTGTTGGAAGAGGCGGCCTCAATTTTCCCTGGGGTGGAATGGGCTTT
GTCTCAGCCCCTGGGATCGCAGGCGGAGATTCCTCGGTTGATGCAAATGG
CGGTGGAGGATTGTGTGGTGGAGCAGAACATTAAGAGTGAGGACCATGCG
GACGGGTCGCCGTTGCCGGAGTTCAATATTTAGGAGGGAGGGATGTAGCG
ATGATTTGTTTCTGGCGTAATGGATGATTTTGATTTTGAAGGTGCGGAAA
GGAGTATATTTTTGTGGTAAGTGTTTTATATGTAGTTTATTTATGCTCTG
TGTTGCAGAAGCTAGCAAAACGAAGTTACCTTTTAGTTTTATGCTTGCGA
GGATTGGAGCGGGAGGGAGGGCGCGTCCCCTACCACTTAGCGTAGCACGA
AGGGGAATGACGAAAAAAAGAATGTAACGTGTATATAAACATTATAATAA
AGAAAAATGTCGCGGTCAATCCGTCCATTTAACAAT
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protein sequence of NO01G02120.1

>NO01G02120.1-protein ID=NO01G02120.1-protein|Name=NO01G02120.1|organism=Nannochloropsis oceanica|type=polypeptide|length=269bp
MCRYSHSSQLLVVALLGTAQLAVHGFLLLNKSPLVQNQDARWRPSPSSDS
LSTRPTTATMHTEGRIWMQFPTLSDTLRVSKGREVVIETTATEHLEEAEI
EVDAHAFQLQQEQQRIREKSQAQAREYKVGVIVVDHGSRRAEANNALAGV
VNDFQALSGYELVEPAHMELAEPSIATAFGRCVERGATFIICHPFFLSRG
RHVAEDIPTLLEEAASIFPGVEWALSQPLGSQAEIPRLMQMAVEDCVVEQ
NIKSEDHADGSPLPEFNI*
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Synonyms
Publications