Candidate sgRNAs were scaned genome-wide for N. oceanica IMET1 (NoIMET1v2). Potential off-target sites were searched using cas-offinder with at most 3 mismatches. All sgRNAs which can uniquely target one specific genomic site and without potential off-target sites within 3 mismatches were retained.
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A. abundant and uniformly distributed target-sites can be obtained along the whole genome.
B. 48% sites were uniquely targeted and without potential off-target sites within 3 mismatches.
C. Average gap length of adjacent targeted sites was ~ 14 bp.