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Overview
Mutinfo:NsbZIP1, basic leucine zipper domain 1 |
Organism:Nannochloropsis salina (N. salina CCMP1776) |
Method:Overexpression |
Genes:NSK004516 |
Reporter:Flag tag |
Promoter:β-tub, UEP |
Selection:Zeocin |
Phenotypes:We overexpressed a bZIP TF, NsbZIP1, in Nannochloropsis salina, and found that transformants showed enhanced growth with concomitant increase in lipid contents. The improved phenotypes were also notable under stress conditions including N limitation and high salt. To understand the mechanism underlying improved phenotypes, we analyzed expression patterns of predicted target genes involved in lipid metabolism via quantitative RT‐PCR, confirming increases transcript levels. NsbZIP1 appeared to be one of type C bZIPs in plants that has been known to regulate lipid metabolism under stress. Taken together, we demonstrated that NsbZIP1 could improve both growth and lipid production, and TF engineering can serve as an excellent genetic engineering tool for production of biofuels and biomaterials in microalgae.
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