delta-12 fatty acid desaturases

Overview


Mutinfo: delta-12 fatty acid desaturases
Organism: Nannochloropsis oceanica (N. oceanica CCMP1779)
Method: Overexpression
Genes: jgi.p\|Nanoce1779_2\|591536
Reporter: Firefly luciferase, NanoLuciferase, Cyan FP (Cerulean variant)
Promoter: EF, LDSP, Ribi
Selection: Hygromycin, Zeocin
Phenotypes: manipulate the EPA biosynthesis pathway
Publications:
Poliner E, Pulman JA, Zienkiewicz K, Childs K, Benning C, Farré EM. A toolkit for Nannochloropsis oceanica CCMP1779 enables gene stacking and genetic engineering of the eicosapentaenoic acid pathway for enhanced long-chain polyunsaturated fatty acid production. Plant biotechnology journal. 2018 01; 16(1):298-309.