EWM27456.1, cds3225 (CDS) Nannochloropsis gaditana

BLAST of EWM27456.1 vs. NCBI_GenBank
Match: gi|585109711|gb|EWM27456.1| (e3 ubiquitin-protein ligase rnf14 [Nannochloropsis gaditana])

HSP 1 Score: 1405.58 bits (3637), Expect = 0.000e+0
Identity = 683/683 (100.00%), Postives = 683/683 (100.00%), Query Frame = 0

Query:    1 MSEEEECQEQQVLEVEALQAILGAGQAGVELDEVSFDVRGDGSTGSPFRLRCEVDIELPAQEAIDIIVTGGTRTSESYREHDDSSENDGEVNAEVACSKNTSAIDPSPQLELRGIKYLSPLTLYLALPSTYPVTCPPELTIEGLWVDEPSSRRIKAELTRMWEEIYHGGPVIYSWMEWLRHDLMEILLVEPILGREVKEKDANDTGTGASEAPRPGEGEALNDLSRRTLILQGSPPQALFTGHNVDEADWSLPKTTHAEVAPTWQSRFRHLVAHAYAKTSAVFLQGTHTCFICFDEQPGLSFVTLQCGHWWCRSCLRSMASTYVTEGGHHLQWLVCPRPDCALEITPDVLRHELLTSEEFARWERLSLQQALDRMEDVAPCPRCQTPCLTEGGRRREGGSDEGKVDGKGRSKTGGADLGECPTCNFVFCPQCQDAYHAGTPCYSSEARLKQVESEIARLMASTQGGRALAPSQNVRLEKLRRAAEESLSRKVVEKDAKSCPACGMSVQKTEGCNKMRCQCGVLWCWKCRKDISTEGYAHFSENATGGCNLWEREALLAWQRQMLMMEGGGMGNGGGGGDRRRAGGDLHGEMVRMLQWGRGWEGEGPGEGRRRRRGRWRGEDANEDGVKLVRCGACHAMNLKTDSNNHRRCGACRRSYCAVCGCHTFRKASDHFGGSKPCRQHS 683
            MSEEEECQEQQVLEVEALQAILGAGQAGVELDEVSFDVRGDGSTGSPFRLRCEVDIELPAQEAIDIIVTGGTRTSESYREHDDSSENDGEVNAEVACSKNTSAIDPSPQLELRGIKYLSPLTLYLALPSTYPVTCPPELTIEGLWVDEPSSRRIKAELTRMWEEIYHGGPVIYSWMEWLRHDLMEILLVEPILGREVKEKDANDTGTGASEAPRPGEGEALNDLSRRTLILQGSPPQALFTGHNVDEADWSLPKTTHAEVAPTWQSRFRHLVAHAYAKTSAVFLQGTHTCFICFDEQPGLSFVTLQCGHWWCRSCLRSMASTYVTEGGHHLQWLVCPRPDCALEITPDVLRHELLTSEEFARWERLSLQQALDRMEDVAPCPRCQTPCLTEGGRRREGGSDEGKVDGKGRSKTGGADLGECPTCNFVFCPQCQDAYHAGTPCYSSEARLKQVESEIARLMASTQGGRALAPSQNVRLEKLRRAAEESLSRKVVEKDAKSCPACGMSVQKTEGCNKMRCQCGVLWCWKCRKDISTEGYAHFSENATGGCNLWEREALLAWQRQMLMMEGGGMGNGGGGGDRRRAGGDLHGEMVRMLQWGRGWEGEGPGEGRRRRRGRWRGEDANEDGVKLVRCGACHAMNLKTDSNNHRRCGACRRSYCAVCGCHTFRKASDHFGGSKPCRQHS
Sbjct:    1 MSEEEECQEQQVLEVEALQAILGAGQAGVELDEVSFDVRGDGSTGSPFRLRCEVDIELPAQEAIDIIVTGGTRTSESYREHDDSSENDGEVNAEVACSKNTSAIDPSPQLELRGIKYLSPLTLYLALPSTYPVTCPPELTIEGLWVDEPSSRRIKAELTRMWEEIYHGGPVIYSWMEWLRHDLMEILLVEPILGREVKEKDANDTGTGASEAPRPGEGEALNDLSRRTLILQGSPPQALFTGHNVDEADWSLPKTTHAEVAPTWQSRFRHLVAHAYAKTSAVFLQGTHTCFICFDEQPGLSFVTLQCGHWWCRSCLRSMASTYVTEGGHHLQWLVCPRPDCALEITPDVLRHELLTSEEFARWERLSLQQALDRMEDVAPCPRCQTPCLTEGGRRREGGSDEGKVDGKGRSKTGGADLGECPTCNFVFCPQCQDAYHAGTPCYSSEARLKQVESEIARLMASTQGGRALAPSQNVRLEKLRRAAEESLSRKVVEKDAKSCPACGMSVQKTEGCNKMRCQCGVLWCWKCRKDISTEGYAHFSENATGGCNLWEREALLAWQRQMLMMEGGGMGNGGGGGDRRRAGGDLHGEMVRMLQWGRGWEGEGPGEGRRRRRGRWRGEDANEDGVKLVRCGACHAMNLKTDSNNHRRCGACRRSYCAVCGCHTFRKASDHFGGSKPCRQHS 683          

BLAST of EWM27456.1 vs. NCBI_GenBank
Match: gi|552816259|ref|XP_005844643.1| (hypothetical protein CHLNCDRAFT_138969 [Chlorella variabilis] >gi|307104286|gb|EFN52541.1| hypothetical protein CHLNCDRAFT_138969 [Chlorella variabilis])

HSP 1 Score: 237.269 bits (604), Expect = 2.341e-65
Identity = 179/582 (30.76%), Postives = 250/582 (42.96%), Query Frame = 0

Query:  115 IKYLSPLTLYLALPSTYPVTCPPELTIEGLWVDEPSSRRIKAELTRMWEEIYHGGPVIYSWMEWLRHDLMEILLVEP--ILGREVKEKDANDTGTG-ASEAPRPGEGEALNDLSRRTLILQGSPPQALFTGHNVDEADWSLPKTTHAEVAPTWQSRFRHLVAHAYAKTSAVFLQGTHTCFICFDEQPGLSFVTLQ-CGHWWCRSCLRSMASTYVTEGGHHLQWLVCPRPDCALEITPDVLRHELLTSEEFARWERLSLQQALDRMEDVAPCPRCQTPCLTEGGRRREGGSDEGKVDGKGRSKTGGADLGECPTCNFVFCPQCQDAYHAGTPCYSSEARLKQVESEIARLMASTQGGRALAPSQNVRLEKLRRAAEESLSRKVVE-------KDAKSCPACGMSVQKTEGCNKMRC-QCGVLWCWKCRKDISTEGYAHFSENATGGCNLWEREALLAWQRQMLMMEGGGMGNGGGGGDRRRAGGDLHGEMVRMLQWGRGWEGEGPGEGRRRRRGRWRGEDANEDGVKLVRCGACHAMNLKTDSNNHRRCGACRRSYCAVC-GCHTFRKASDHFGGSKPCRQHS 683
            ++YL P+ + L L   YP   PP +++  LW+      R++ +L R+W E   GGPV Y+W +WL+   ++ L  E   +L  +    D +  G G A+  PR G                G P      G +  E           EVA   + R   L+ +   +  A F +  HTC +C +E PG +FV L+ C H WC  CL   A  +V EGG  L+ L CP P C   + P VLR  +L+ ++F RWE+L+LQ+ LD M D A CPRC +  L +                            +CPTC FVFC  C + +H GT C S+E +L  V  ++A       GGRA        ++ LRR  +E LS   +E       K +K CP CGM+ QK EGCNKM C  CG  WCW+C K+I  +GY HF    +G C L++   +L W+ Q   M+             R     L  EM                                           C  +N K  +NNH  C +C   +C  C      R A     G + C+QHS
Sbjct:  176 VQYLPPICMQLRLGGGYPGQEPPGVSLSALWLGAAQVARLQRQLLRLWHEQGPGGPVCYTWADWLQSSALQHLGGEQALLLADDAPSSDGSTNGGGVAAGEPRAGS--------------SGRPDSPGAEGGDEVEQ----------EVA---EERLVKLLRYDAVQEHAAFQRARHTCGVCLEEAPGTAFVRLEGCRHAWCALCLAEQARIHVAEGG--LEKLRCPDPGCGAALAPGVLRR-VLSPDDFGRWEQLTLQRTLDTMPDAAYCPRCSSLALEDAD-----------------------SCAQCPTCLFVFCSLCNEGWHPGTTCVSAETKLAMVRRKLA------GGGRA-------AVDDLRRQEQELLSLAQIEAGWAALCKMSKRCPQCGMATQKAEGCNKMACGGCGAYWCWRCGKEI--DGYRHFR---SGQCILFDEAEILRWEAQWEEMQAAA----------RVMAAGLRNEM-------------------------------------------CGQVNHKIGNNNHLACWSCTSHFCYCCRAVLRGRGAGGSHFGPRGCKQHS 633          

BLAST of EWM27456.1 vs. NCBI_GenBank
Match: gi|902225615|gb|KNA20123.1| (hypothetical protein SOVF_055230 [Spinacia oleracea])

HSP 1 Score: 232.646 bits (592), Expect = 2.006e-63
Identity = 168/586 (28.67%), Postives = 242/586 (41.30%), Query Frame = 0

Query:  100 NTSAIDPSPQLELRGIKYLSPLTLYLALPSTYPVTCPPELTIEGLWVDEPSSRRIKAELTRMWEEIYHGGPVIYSWMEWLRHDLMEILLVEPILGREVKEKDANDTGTGASEAPRPGEGEALNDLSRRTLILQGSPPQALFTGHNVDEADWSLPKTTHAEVAPTWQSRFRHLVAHAYAKTSAVFLQGTHTCFICFDEQPGLSFVTLQCGHWWCRSCLRSMASTYVTEGGHHLQWLVCPRPDCALEITPDVLRHELLTSEEFARWERLSLQQALDRMEDVAPCPRCQTPCLTEGGRRREGGSDEGKVDGKGRSKTGGADLGECPTCNFVFCPQCQDAYHAGTPCYSSEARLKQVES-EIARLMASTQGGRALAPSQNVRLEKLRRAAEESLSRKVVEKDAKSCPACGMSVQKTEGCNKMRC-QCGVLWCWKCRKDISTEGYAHFSENATGGCNLWEREALLAWQRQMLMMEGGGMGNGGGGGDRRRAGGDLHGEMVRMLQWGRGWEGEGPGEGRRRRRGRWRGEDANEDGVKLVRCGACHAMNLKTDSNNHRRCGACRRSYCAVCGCHTFRKASDHFGGSKPCRQHS 683
            N+   D    L    ++YL P+ L   LP +YP  CPP  TI   W+ E    ++ + L  +W E   G  ++Y W+EWL    +  L                    G+ E    G     +   RR      SP             D  +P                 + ++   +    FL G H C ICF E  G  F+ L C H++C  C+ + A  +V EG   +  L CP   C   I P +L+  LL  EEF RWE L LQ++LD M DVA CPRC+TPCL +  +                         +C  C F FC  C++  H G  C + E +LK +E  + + L+   Q  + L     +R            S K + +DAK CP C +++ +TEGCNKM C QCG  +C++C K IS  GY HF E   GGC L+ +EA++ W+ Q+               + R+  G +  +M                                        C  C  +N K  +NNH  C AC+  YC +C     R++S+H+ G K C+QHS
Sbjct:  222 NSKVCDSDDFLYTFKVQYLPPIILTCLLPKSYPSHCPPSFTISVQWLHESRISKLCSMLDLLWNE-QAGQEILYQWVEWLHSSSLSYL--------------------GSDEELLLGPYNVKSVPDRRAFSGSLSP-------------DIDIPS----------------MRSYNEERVHENFLSGCHECCICFSEYAGTEFLRLPCHHFFCVKCMTTYADVHVKEGT--VNKLQCPSTKCGGMIPPGLLKR-LLGDEEFQRWESLVLQKSLDAMPDVAYCPRCETPCLEDDDQH-----------------------AQCSKCFFSFCSLCREKRHVGIACMTPEMKLKFLEERQNSSLIKDEQRRKELDMINELR------------SVKEILRDAKQCPNCKIAISRTEGCNKMVCTQCGKYFCYRCSKAIS--GYEHFREE--GGCQLFPQEAIVQWEEQV---------------NARQVLGQIQAQMF---------------------------------AANARPCPGCKQLNAKMGNNNHIFCWACQNHYCYLCR-KMVRRSSEHY-GPKGCKQHS 665          

BLAST of EWM27456.1 vs. NCBI_GenBank
Match: gi|971520247|dbj|GAQ78006.1| (hypothetical protein KFL_000060730 [Klebsormidium nitens])

HSP 1 Score: 233.032 bits (593), Expect = 3.313e-63
Identity = 176/572 (30.77%), Postives = 237/572 (41.43%), Query Frame = 0

Query:  115 IKYLSPLTLYLALPSTYPVTCPPELTIEGLWVDEPSSRRIKAELTRMWEEIYHGGPVIYSWMEWLRHDLMEILLVEPILGREVKEKDANDTGTGASEAPRPGEGEALNDLSRRTLILQGSPPQALFTGHNVDEADWSLPKTTHAEVAPTWQSRFRHLVAHAYAKTSAVFLQGTHTCFICFDEQPGLSFVTL--QCGHWWCRSCLRSMASTYVTEGGHHLQWLVCPRPDCALEITPDVLRHELLTSEEFARWERLSLQQALDRMEDVAPCPRCQTPCLTEGGRRREGGSDEGKVDGKGRSKTGGADLGECPTCNFVFCPQCQDAYHAGTPCYSSEARLKQVESEIARLMASTQGGRALAPSQNVRLEKLRRAAEESLSRKVVEKDAKSCPACGMSVQKTEGCNKMRC-QCGVLWCWKCRKDISTEGYAHFSENATGGCNLWEREALLAWQRQMLMMEGGGMGNGGGGGDRRRAGGDLHGEMVRMLQWGRGWEGEGPGEGRRRRRGRWRGEDANEDGVKLVRCGACHAMNLKTDSNNHRRCGACRRSYCAVCGCHTFRKASDHFGGSKPCRQHS 683
            +++L PL L   LP+ YP   PP   +  +W+  P   R+   L  +W+E   G  V+YSW +WL  +  E L +                          GE   L      TL  +G   ++           W +            Q     L+ +   KT   F +G H C +CF EQPG  FV L   C H +CR C+ S+   +V EG   L  L CP  DC   + P++++ +LL  E+F RWE L LQ+  D M DV  CPRC+T  + E                            +C  C F FC  C DA H G+ C  +EA+L       A L   T+G R  A  Q  +   L     E L+ K VE++AK CP CGM++QK EGCNKM C  C   +C+KC K IS  GY HF+   +G C L+E   + AW R M     G         DR  A   ++   VR                                      C AC   N+K   NNH RC +C+  +C  CG     K S HF G K C+QH+
Sbjct:  262 LEHLPPLRLTCTLPAAYPSHSPPRFALSSVWLTRPQLGRLCRGLDEVWQE-NQGQVVVYSWADWLTRETFETLDL--------------------------GESFELGG----TLNAEGKGKESAEHDERAQAEGWGV------------QDVLSRLLRYNEEKTMEEFRKGVHECQVCFAEQPGTEFVHLWPGCRHNFCRGCMTSLVDIHVKEGS--LARLTCPDTDCKEPLPPNIIK-DLLAPEQFQRWETLMLQKTFDSMSDVVYCPRCETATIEEPDH-----------------------TAQCQKCFFAFCSLCDDATHFGSQCMDAEAQL-------AVLRERTRG-RLPAEEQKRKEAAL---LNEILNMKYVEREAKKCPKCGMAIQKDEGCNKMTCWNCSSYFCYKCGKAIS--GYDHFN---SGACVLFEAAEIDAWNRMM----AGVNAPRNEARDRIDAQIQMYPNRVRA-------------------------------------CPACRQPNVKEAGNNHLRCWSCQNHFCGACG-KAVLKGSHHF-GPKGCKQHT 705          

BLAST of EWM27456.1 vs. NCBI_GenBank
Match: gi|1021515369|ref|XP_016201136.1| (E3 ubiquitin-protein ligase RNF14 [Arachis ipaensis])

HSP 1 Score: 221.476 bits (563), Expect = 2.788e-59
Identity = 162/573 (28.27%), Postives = 239/573 (41.71%), Query Frame = 0

Query:  115 IKYLSPLTLYLALPSTYPVTCPPELTIEGLWVDEPSSRRIKAELTRMWEEIYHGGPVIYSWMEWLRHDLMEILLV--EPILGREVKEKDANDTGTGASEAPRPGEGEALNDLSRRTLILQGSPPQALFTGHNVDEADWSLPKTTHAEVAPTWQSRFRHLVAHAYAKTSAVFLQGTHTCFICFDEQPGLSFVTLQCGHWWCRSCLRSMASTYVTEGGHHLQWLVCPRPDCALEITPDVLRHELLTSEEFARWERLSLQQALDRMEDVAPCPRCQTPCLTEGGRRREGGSDEGKVDGKGRSKTGGADLGECPTCNFVFCPQCQDAYHAGTPCYSSEARLKQVESEIARLMASTQGGRALAPSQNVRLEKLRRAAEESLSRKVVEKDAKSCPACGMSVQKTEGCNKMRC-QCGVLWCWKCRKDIS-TEGYAHFSENATGGCNLWEREALLAWQRQMLMMEGGGMGNGGGGGDRRRAGGDLHGEMVRMLQWGRGWEGEGPGEGRRRRRGRWRGEDANEDGVKLVRCGACHAMNLKTDSNNHRRCGACRRSYCAVCGCHTFRKASDHFGGSKPCRQHS 683
            ++YL P+ L   LP +YP   PP  T+   W++      + ++L  +W E   G  VIY W++WL+   +  L    E ILGR          G       R   G         ++ L  + P     G+N         K  H E                       FL+  H C ICF E PG  FV L C H++C  CL++    +V EG   +  L CP   C + I P +L+H LL  EE+ RWE + L++ L  M DV  CPRC+TPC+ +  +                         +C  C F FC  C++  H G  C + + +L+        ++   Q    L   Q +R    R    E LS K + +D+K CP+C M++ +TEGCNKM+C  CG  +C++C K I  ++ Y HF +   G C L+ +E +  WQ ++               + R+  G L  E+                    R RG              + C  C   N K  +NNH  C AC+  YC +C     R+ S H+ G K C+QH+
Sbjct:  243 VQYLPPIVLTCLLPKSYPSHQPPLFTVSVQWLESLDILSLCSQLDSIWAE-QQGQEVIYQWVDWLQSSSLSHLGFDKEIILGR---------NGVNCVGDERVVSG---------SISLDAAIP--FLRGYN---------KERHNEN----------------------FLKELHECSICFSEYPGTEFVRLPCQHFFCLKCLQTFTQMHVKEGT--ISNLQCPDSKCKVMIPPGLLKH-LLGDEEYERWESMMLERTLASMSDVVYCPRCETPCIEDEDQH-----------------------AQCSKCFFSFCTLCRERRHVGIACMTLDMKLQ--------ILQDRQNSSQLKEDQKLR---EREKINEMLSMKEIHRDSKLCPSCSMAINRTEGCNKMKCSNCGAYFCYRCNKLIDPSDPYGHFRD---GSCELFPQEMIENWQERI---------------NHRQVVGQLQAELF-------------------RERG--------------LPCPNCRQFNAKVGNNNHMFCWACQSHYCYLCN-EIVRRGSKHY-GPKGCKQHT 673          

BLAST of EWM27456.1 vs. NCBI_GenBank
Match: gi|873241170|emb|CEL92756.1| (unnamed protein product [Vitrella brassicaformis CCMP3155])

HSP 1 Score: 218.394 bits (555), Expect = 2.841e-58
Identity = 163/563 (28.95%), Postives = 248/563 (44.05%), Query Frame = 0

Query:    3 EEEECQEQQVLEVEALQAILGAGQAGVELDEVSFDVRGDGSTGSPFRLRCEVDIELPAQEAIDIIVTGGTRTSESYREHDDSSENDGEVNAEVACSKNTSAIDPSPQLELRGIKYLSPLTLYLALPSTYPVTCPPELTIEGLWVDEPSSRRIKAELTRMWEEIYHGGPVIYSWMEWLRHDLMEILLVEPILGREVKEKDANDTGTGASEAPRPGEGEALNDLSRRTLILQGSPPQALFTGHNVDEADWSLPKTTHAEVAPTWQSRFRHLVAHAYAKTSAVFLQGTHTCFICFDEQPGLSFVTLQ-CGHWWCRSCLRSMASTYVTEGGHHLQWLVCPRPDCALEITPDVLRHELLTSEEFARWERLSLQQALDRMEDVAPCPRCQTPCLTEGGRRREGGSDEGKVDGKGRSKTGGADLGECPTCNFVFCPQCQDAYHAGTPCYSSEARLKQVESEIARLMASTQGGRALAPSQNVRLEKLRRAAEESLSRKVVEKDAKSCPACGMSVQKTEGCNKMRC-QCGVLWCWKCRKDISTEGYAHFSENATGGCNLWEREALLAWQRQM 563
            E E  +  Q  E+ AL++I       +  D+     + D     P         + P+ EAID+I     R  E   +H  +   D +    +   +    I          +++L PL+L++ +P  YP    P  T+  +W+D+ +  R+   L ++WEE   G P++++W  +L  D M  L     +GR +      + G         G+G    D            P+A+    +V                         L+ +  ++    +    HTC ICFD   G     L  C H +C  CL  +  T+V +G  +L  L CP P C   I P+VL H LL  +E+ RW+RLSLQ+ALD M D+  CPRC+         R E  +     D           L  CP C+F FC  C+  +H GT C   E  L+ +E       A ++G +A       R + L++ AE   S K ++++A+ CP CGM++ KTEGCNKM+C QC   +CWKC + IS  GY HF+ N    CNL++ E +  W R M
Sbjct:   56 EREANERAQEDEIMALESIYMEAFKAISTDKSPRVFQLDVEVTFP---DAPSSADAPSDEAIDVI----ARFEEPSAQHHPNYAGDEDHPDRLRIEQQDVPIG--------RVRFLPPLSLHIVMPPAYPSRRSPFFTLSAMWIDKGAVGRLCRRLDQLWEEEGPGSPIVFTWANFLSTDSMGYL----SMGRLIVIAPLREEG---------GDGHTTGD------------PRAMQECADVGVC-------------------VEELLRYDASREDVEYRSTKHTCMICFDVMLGERMDRLPGCRHAYCTQCLAGLVKTHVKDGAINL--LRCPEPSCGRSIDPEVL-HRLLDEDEYERWQRLSLQKALDGMGDLVYCPRCE-------AHRNENTAVLEDAD----------HLARCPKCHFAFCGICRAEWHPGTACLDPEQLLEVLE-------ARSKGDKAYGEEVLRREQNLKQEAE---SLKTIKREARQCPTCGMAIYKTEGCNKMKCEQCYTNFCWKCLQPIS--GYEHFANNPN--CNLFDMEEIERWNRMM 525          

BLAST of EWM27456.1 vs. NCBI_GenBank
Match: gi|508777891|gb|EOY25147.1| (NDR1/HIN1-like 8, putative isoform 1 [Theobroma cacao])

HSP 1 Score: 215.698 bits (548), Expect = 3.918e-57
Identity = 154/570 (27.02%), Postives = 240/570 (42.11%), Query Frame = 0

Query:  115 IKYLSPLTLYLALPSTYPVTCPPELTIEGLWVDEPSSRRIKAELTRMWEEIYHGGPVIYSWMEWLRHDLMEILLVEPILGREVKEKDANDTGTGASEAPRPGEGEALNDLSRRTLILQGSPPQALFTGHNVDEADWSLPKTTHAEVAPTWQSRFRHLVAHAYAKTSAVFLQGTHTCFICFDEQPGLSFVTLQCGHWWCRSCLRSMASTYVTEGGHHLQWLVCPRPDCALEITPDVLRHELLTSEEFARWERLSLQQALDRMEDVAPCPRCQTPCLTEGGRRREGGSDEGKVDGKGRSKTGGADLGECPTCNFVFCPQCQDAYHAGTPCYSSEARLKQVESEIARLMASTQGGRALAPSQNVRLEKLRRAAEESLSRKVVEKDAKSCPACGMSVQKTEGCNKMRCQ-CGVLWCWKCRKDISTEGYAHFSENATGGCNLWEREALLAWQRQMLMMEGGGMGNGGGGGDRRRAGGDLHGEMVRMLQWGRGWEGEGPGEGRRRRRGRWRGEDANEDGVKLVRCGACHAMNLKTDSNNHRRCGACRRSYCAVCGCHTFRKASDHFGGSKPCRQHS 683
            ++YL P+ L   LP +YP   PP  TI   W+       + ++L  +W+E   G  VIY W +WL++  +  L                    G  +    G     N+  RR +    SP           + D    ++ + E+                      FL+  H C ICF E  G+ F+ L C H++C  C+++ +  ++TEG   +  L+CP   C   + P +L+  LL  E + RWE L LQ+ L+ M DV  CPRC+TPC+ +  +                         +C  C F FC  C++  H G  C + E +L        R++   Q    L  +Q     K R    E LS K + +DAK CP+C M++ KTEGCNKM C+ CG  +C++C   I+  GY HF +   G C L+ +E +  W+ ++               + R+  G +H ++                      RG+               C  C  +N K  +NNH  C AC+  YC +C  +  R++S H+ G K C+QH+
Sbjct:  249 VQYLPPIVLTCLLPRSYPSHLPPYFTISVQWLHSARISDLCSQLDLIWKE-QEGQEVIYRWADWLQNFSLSYL--------------------GCDKEIMLGPYGIENNGERRAVSGSVSP-----------DVDVPFIRSYNDEIC------------------HENFLESLHECSICFSEYAGIDFIRLPCQHFFCCKCIKTYSDMHITEGT--VNKLLCPEAKCGGTVPPGLLK-RLLGDEGYERWESLMLQKTLESMSDVVYCPRCETPCIEDEDQH-----------------------AQCSKCFFSFCTLCRERRHVGIECMTPELKL--------RILQERQNSSQLNQAQK---HKEREMINEILSMKEIMRDAKQCPSCKMAISKTEGCNKMVCENCGQYFCYRCNSAIN--GYDHFRD---GACELFPQEMIREWEERL---------------NARQVLGQVHAQLF-------------------AERGQ--------------PCPNCRQLNAKVGNNNHIFCWACQMHYCYLCK-NIVRRSSQHY-GPKGCKQHT 676          

BLAST of EWM27456.1 vs. NCBI_GenBank
Match: gi|297851676|ref|XP_002893719.1| (E3 ubiquitin-protein ligase RNF14 [Arabidopsis lyrata subsp. lyrata] >gi|297339561|gb|EFH69978.1| predicted protein [Arabidopsis lyrata subsp. lyrata])

HSP 1 Score: 214.157 bits (544), Expect = 1.413e-56
Identity = 174/616 (28.25%), Postives = 253/616 (41.07%), Query Frame = 0

Query:   78 YREHDDSSENDGEVNAEVACSKNTSA-----IDPSPQLE--LRGIK--YLSPLTLYLALPSTYPVTCPPELTIEGLWVDEPSSRRIKAELTRMWEEIYHGGPVIYSWMEWLRHDLMEILLVEPILGREVKEKDANDTGTGASEAPRPGEGEALNDLSRRTLILQGSPPQALFTGHNVDEADWSLPKTTHAEVAPTWQSRFRHLVAHAYAKTSAVFLQGTHTCFICFDEQPGLSFVTLQCGHWWCRSCLRSMASTYVTEGGHHLQWLVCPRPDCALEITPDVLRHELLTSEEFARWERLSLQQALDRMEDVAPCPRCQTPCLTEGGRRREGGSDEGKVDGKGRSKTGGADLGECPTCNFVFCPQCQDAYHAGTPCYSSEARLKQVESEIARLMASTQGGRALAPSQNVRLEKLRRAAEESLSRKVVEKDAKSCPACGMSVQKTEGCNKMRC-QCGVLWCWKCRKDISTEGYAHFSENATGGCNLWEREALLAWQRQMLMMEGGGMGNGGGGGDRRRAGGDLHGEMVRMLQWGRGWEGEGPGEGRRRRRGRWRGEDANEDGVKLVRCGACHAMNLKTDSNNHRRCGACRRSYCAVCGCHTFRKASDHFGGSKPCRQHS 683
            +  H D       VN E     N SA      D S + E  L   K  +L P+ L   LP+ YP   PP   I   W++      + + L  +W E   G  V+Y W +WL++  +  L  +       KE      G   S   R   G      SR       SP             D  +P                ++ ++   K    FLQ  H C ICF E  G+ FV L C H++C  C+++    +V+EG   +  L+CP   C   + P +L+  LL  E + RWE L LQ+ L+ M DVA CPRC+TPC+           DE +             L  C  C F FC  C++  H G  C S E RL+        ++   QG   L   Q     K +    E +S KV+ K AK CP+C +++ +T GCNKM C  CG  +C++C K I+  GY HF E   G C+L+ +EA+  W  +M               + R+  G +  ++           G+ P  G+                     C  C   N K  +NNH  C AC+  +C +C     +K++ H+ G K C+QH+
Sbjct:  201 FDRHKDMRYFQVHVNVEATSEYNISAKLKLQADSSKESEDFLYSFKAQHLPPIVLTCLLPNAYPSHLPPYFLISVQWMNPDKISSLCSMLDSIWME-QPGQEVLYLWTDWLQNSSISHLGFD-------KEIFLGPYGVTCSRDKRAVSG------SR-------SP-------------DSDIP----------------YIRSYDDEKRHESFLQSLHECCICFTESAGIDFVKLPCQHFFCLKCMKTYTDIHVSEGT--VNKLMCPDSKCGEIVPPGILK-RLLGEEAYERWETLMLQKTLESMTDVAYCPRCETPCI----------EDEEQ-------------LALCFKCYFSFCTLCKEKRHVGVACMSPELRLQ--------ILQERQGSSRLGEEQR---RKEKEMINEIMSVKVIMKSAKQCPSCKIAISRTGGCNKMVCNNCGQYFCYRCNKAIT--GYEHFRE---GTCDLFPQEAIQEWNERM---------------NERQVIGQIQAQLF-------AQHGQFPQRGQ--------------------LCPNCRQFNAKAGNNNHLFCWACQAHFCYLCK-KVVKKSAQHY-GPKGCKQHT 680          

BLAST of EWM27456.1 vs. NCBI_GenBank
Match: gi|242074180|ref|XP_002447026.1| (E3 ubiquitin-protein ligase RNF14 [Sorghum bicolor] >gi|241938209|gb|EES11354.1| hypothetical protein SORBI_3006G199200 [Sorghum bicolor])

HSP 1 Score: 211.846 bits (538), Expect = 4.673e-56
Identity = 190/676 (28.11%), Postives = 278/676 (41.12%), Query Frame = 0

Query:    9 EQQVLEVEALQAILGAGQAGVELDEVSFDVRGDGSTGSPFRLRCEVDIELPAQEAIDIIVTGGTRTSESYREHDDSSENDGEVNAEVACSKNTSAIDPSPQLELRGIKYLSPLTLYLALPSTYPVTCPPELTIEGLWVDEPSSRRIKAELTRMWEEIYHGGPVIYSWMEWLRHDLMEILLVEPILGREVKEKDANDTGTGASEAPRPGEGEALNDLSRRTLILQGSPPQALFTGHNVDEADWSLPKTTHAEVAPTWQSRFRHLVAHAYAKTSAVFLQGTHTCFICFDEQPGLSFVTLQCGHWWCRSCLRSMASTYVTEGGHHLQWLVCPRPDCALEITPDVLRHELLTSEEFARWERLSLQQALDRMEDVAPCPRCQTPCLTEGGRRREGGSDEGKVDGKGRSKTGGADLGECPTCNFVFCPQCQDAYHAGTPCYSSEARLKQVESEIARLMASTQGGRALAPSQNVRLEKLRRAAEESLSRKVVEKDAKSCPACGMSVQKTEGCNKMRC-QCGVLWCWKCRKDISTEGYAHFSENATGGCNLWEREALLAWQRQMLMMEGGGMGNGGGGGDRRRAGGDLHGEMVRMLQWGRGWEGEGPGEGRRRRRGRWRGEDANEDGVKLVRCGACHAMNLKTDSNNHRRCGACRRSYCAVCGCHTFRKASDHFGGSKPCRQHS 683
            ++Q  E+ AL+AI G   A V LD            G     +  V IE+P  ++ID+       T  +Y         DG +N    C  +          + R +++L P+ L   LP++YP   PP  TI   W+D+     +   L  +WEE   G  V Y W++WL+   +  L  +   G  + + D      G  +   P                  +PP            D  +P+                L+ +   K    FL   H C ICF E PG+ F+ L C H++C  C+++     V EG  ++  L+CP   C   + P+VL+  LL  +E+ RWE L LQ+ LD M+DV  CPRCQT CL + G                           C +C F FC  C++  H G  C S E RL  +E                  S NV+ +++ +  EE  S K + KD+K CP C M++ KTEGCNKM C  CG  +C++C + I+  GY HF     G C L+ +E L  W+ QM                RR+     H EM        G +G+                           C  C   + K  +NNH  C AC++ +CA+C   T +K + H+ G K C+QHS
Sbjct:  144 QRQEDEICALEAIFG--DAVVILDR----------KGGQRSFQVLVHIEIP--DSIDV------STKLNY--------GDGTLNYGATCDDDDDLF-----YKFR-VEHLPPILLTCLLPTSYPSHRPPFFTISTYWLDKRMISLLCRMLDMLWEE-QQGMEVAYQWVQWLQSSSLSHLGFDN--GVILSKSDVTCDADGEDKRACP----------------DNAPP------------DVIIPR----------------LMRYNDNKHHEAFLHAIHDCMICFSECPGVDFIKLPCHHFFCWKCMQTYCKMNVKEG--NVVKLLCPDTKCEGAVPPNVLK-RLLEEDEYERWEGLLLQRTLDAMKDVVYCPRCQTACLEDVGNE-----------------------AVCSSCLFSFCTLCRNRRHVGEQCMSPEERLMILEKR--------------QESGNVQGDQM-KILEELRSLKEIMKDSKQCPKCRMAISKTEGCNKMHCGNCGEYFCYQCNRAIT--GYEHFR----GSCVLFPQEELDRWELQM------------NQRVRRQVVAHAHAEM-------HGRDGQAHP------------------------CPTCRQPSPKIGNNNHLFCWACQKHFCALCH-KTVQKPAQHY-GPKGCKQHS 646          

BLAST of EWM27456.1 vs. NCBI_GenBank
Match: gi|695026879|ref|XP_009400756.1| (PREDICTED: E3 ubiquitin-protein ligase RNF14 [Musa acuminata subsp. malaccensis])

HSP 1 Score: 209.149 bits (531), Expect = 2.507e-55
Identity = 196/691 (28.36%), Postives = 284/691 (41.10%), Query Frame = 0

Query:    3 EEEECQEQQVL--------EVEALQAILGAGQAGVELDEVSFDVRGDGSTGSPFRLRCEVDIELPAQEAIDIIVTGGTRTSESYREHDDSSENDGEVNAEVACSKNTSAIDPSPQLELRGIKYLSPLTLYLALPSTYPVTCPPELTIEGLWVDEPSSRRIKAELTRMWEEIYHGGPVIYSWMEWLRHDLMEILLVEP-ILGREVKEKDANDTGTGASEAPRPGEGEALNDLSRRTLILQGSPPQALFTGHNVDEADWSLPKTTHAEVAPTWQSRFRHLVAHAYAKTSAVFLQGTHTCFICFDEQPGLSFVTLQCGHWWCRSCLRSMASTYVTEGGHHLQWLVCPRPDCALEITPDVLRHELLTSEEFARWERLSLQQALDRMEDVAPCPRCQTPCLTEGGRRREGGSDEGKVDGKGRSKTGGADLGECPTCNFVFCPQCQDAYHAGTPCYSSEARLKQVESEIARLMASTQGGRALAPSQNVRLEKLRRAAEESLSRKVVEKDAKSCPACGMSVQKTEGCNKMRCQ-CGVLWCWKCRKDISTEGYAHFSENATGGCNLWEREALLAWQRQMLMMEGGGMGNGGGGGDRRRAGGDLHGEMVRMLQWGRGWEGEGPGEGRRRRRGRWRGEDANEDGVKLVRCGACHAMNLKTDSNNHRRCGACRRSYCAVCGCHTFRKASDHFGGSKPCRQHS 683
            EE E  E+Q+         EV AL+AI G        + +SFD + +G     F++R E D  LP     D I       S + +    S   +G           T + D    L    ++YL PL L   LP +YP   PP  TI   W++      +  +L  +W E   G  + Y W++WL++  +  L  +  ++      +D  D         R   G A  D    ++I             N D+                              K    FL   H C ICF E  G SF+ L C H++C  CL + ++ +V EG   +  L+CP   C   + P +L+  LL SE + RWE L LQ+ LD M DVA CPRC+T CL +                            +C  C F FC  C++  H G  C + E +L+        ++ + Q    L+ +Q   L   R    E LS K   +DAK CP+C M++ +TEGCNKM CQ CG  +C++C K I  +GY HF E    GC L+ RE +  W+ QM               + R+  G +  E+              P  G                      C  C  +N K  +NNH  C AC++ YCA+C   T RK+S+HFG ++ C+QH+
Sbjct:   85 EEPELSEEQIRVNDQMQEDEVLALEAIYGD-------NVLSFD-KKEGLRF--FQVRMEYD--LP-----DCINVSAMLQSSTTKVKSGSRSLNGR----------TKSDDSHEFLYTFNVQYLPPLLLTCLLPKSYPSHRPPYFTITVDWLNSEKISSLCHKLDAIWME-QPGQEITYQWVDWLQNYSLSYLGFDSGVMLDRYDMQDVKDI--------RADFGNARPDFIISSMI-------------NYDQE-----------------------------KCHQSFLNNLHQCIICFTEYAGTSFIKLPCQHFFCWKCLETYSNMHVMEGT--VTKLLCPTDRCGGFVPPGLLK-RLLGSEAYKRWESLVLQKTLDSMTDVAYCPRCETACLEDEEHH-----------------------AQCSKCFFSFCSLCRERRHVGVECMTLEVKLQ--------ILQARQNSSNLSRNQ---LHMEREMINEILSVKEALRDAKQCPSCKMAISRTEGCNKMVCQNCGQYFCYRCNKAI--DGYDHFRE----GCELFPREEIERWEIQM---------------NPRQVVGQIRAELY-------------PNVGH--------------------PCPTCRQVNPKVGNNNHIFCWACQKHYCAMCR-KTVRKSSEHFGPNR-CKQHT 604