Deletion mutants for nitrate reductase

Mutinfo:

Method:

Phenotypes:

The isolated mutants, in which precise deletion of five bases caused a frameshift in NR translation, grow normally under NH4 Cl but fail to grow under NaNO3 , and thus represent a valuable chassis strain for transgenic-strain development.

• Wang Q, Lu Y, Xin Y, Wei L, Huang S, Xu J. Genome editing of model oleaginous microalgae Nannochloropsis spp. by CRISPR/Cas9. The Plant journal : for cell and molecular biology. 2016 12; 88(6):1071-1081.