Genome-wide searching for potential off-target sites of all Cas9 RNA-guided endonucleases (20bp-NGG)


Candidate sgRNAs were scaned genome-wide for N. oceanica IMET1 (NoIMET1v2).  Potential off-target sites were searched using cas-offinder with at most 3 mismatches. All sgRNAs which can uniquely target one specific genomic site and without potential off-target sites within 3 mismatches were retained.