diacylglycerol acyltransferase type 2–7

Overview


Mutinfo: diacylglycerol acyltransferase type 2–7
Organism: Nannochloropsis oceanica (N. oceanica CCMP1779)
Method: Overexpression
Genes: jgi.p\|Nanoce1779_2\|584523
Reporter: Flag tag
Promoter: Hsp20
Selection: Zeocin
Phenotypes: Overexpression of DGAT2 in Nannochloropsis increased the relative transcript abundance by 3.48-fold in engineered microalgae cells. TAG biosynthesis was subsequently accelerated by DGAT2 overexpression and neutral lipid content was significantly elevated by 69% in engineered microalgae.
Publications:
Li DW, Cen SY, Liu YH, Balamurugan S, Zheng XY, Alimujiang A, Yang WD, Liu JS, Li HY. A type 2 diacylglycerol acyltransferase accelerates the triacylglycerol biosynthesis in heterokont oleaginous microalga Nannochloropsis oceanica. Journal of biotechnology. 2016 Jul 10; 229:65-71.