A type 2 diacylglycerol acyltransferase accelerates the triacylglycerol biosynthesis in heterokont oleaginous microalga Nannochloropsis oceanica

Overview
TitleA type 2 diacylglycerol acyltransferase accelerates the triacylglycerol biosynthesis in heterokont oleaginous microalga Nannochloropsis oceanica
AuthorsLi DW, Cen SY, Liu YH, Balamurugan S, Zheng XY, Alimujiang A, Yang WD, Liu JS, Li HY
TypeJournal Article
Journal NameJournal of biotechnology
Volume229
IssueN/A
Year2016
Page(s)65-71
CitationLi DW, Cen SY, Liu YH, Balamurugan S, Zheng XY, Alimujiang A, Yang WD, Liu JS, Li HY. A type 2 diacylglycerol acyltransferase accelerates the triacylglycerol biosynthesis in heterokont oleaginous microalga Nannochloropsis oceanica. Journal of biotechnology. 2016 Jul 10; 229:65-71.

Abstract

Oleaginous microalgae have received a considerable attention as potential biofuel feedstock. However, lack of industry-suitable strain with lipid rich biomass limits its commercial applications. Targeted engineering of lipogenic pathways represents a promising strategy to enhance the efficacy of microalgal oil production. In this study, a type 2 diacylglycerol acyltransferase (DGAT), a rate-limiting enzyme in triacylglycerol (TAG) biosynthesis, was identified and overexpressed in heterokont oleaginous microalga Nannochloropsis oceanica for the first time. Overexpression of DGAT2 in Nannochloropsis increased the relative transcript abundance by 3.48-fold in engineered microalgae cells. TAG biosynthesis was subsequently accelerated by DGAT2 overexpression and neutral lipid content was significantly elevated by 69% in engineered microalgae. The fatty acid profile determined by GC-MS revealed that fatty acid composition was altered in engineered microalgae. Saturated fatty acids and polyunsaturated fatty acids were found to be increased whereas monounsaturated fatty acids content decreased. Furthermore, DGAT2 overexpression did not show negative impact on algal growth parameters. The present investigation showed that the identified DGAT2 would be a potential candidate for enhancing TAG biosynthesis and might facilitate the development of promising oleaginous strains with industrial potential.

Properties
Additional details for this publication include:
Property NameValue
Journal CountryNetherlands
Publication TypeJournal Article
Language Abbreng
LanguageEnglish
CopyrightCopyright © 2016 Elsevier B.V. All rights reserved.
PIIS0168-1656(16)30261-9
ElocationS0168-1656(16)30261-9
DOI10.1016/j.jbiotec.2016.05.005
Journal AbbreviationJ. Biotechnol.
Publication Date2016 Jul 10
eISSN1873-4863
ISSN1873-4863
Publication ModelPrint-Electronic
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PMID: PubMedPMID:27164260