EWM25480.1, cds5199 (CDS) Nannochloropsis gaditana

BLAST of EWM25480.1 vs. NCBI_GenBank
Match: gi|585107243|gb|EWM25480.1| (trna-splicing ligase [Nannochloropsis gaditana])

HSP 1 Score: 1058.51 bits (2736), Expect = 0.000e+0
Identity = 510/510 (100.00%), Postives = 510/510 (100.00%), Query Frame = 0

Query:    1 MANGSVVRTFEEEMGFLHRETPTRFRIDKGCVPNMKVEGAFYVNTKLEELMFAELKQHTTAGGVGGFLPAVKQIANVAALPGIVGRSIGLPDVHSGYGFAIGNVAAFAVADTEAIVSPGGVGFDINCGVRLIRTNLTEEDVRGKREKLTQSLFDHIPVGVGSKGVIPTSAADLEAALEMGMDWSLREGYAWAEDNEHCEENGRMRQADPTKVGMRAKKRGLPQLGTLGAGNHYAEIQVVEEIHDPRAAARMGIDQIGQVCVMIHSGSRGLGHQVATDALVEMERAMARDGILTNDRQLACARIQSPEGQNYLAAMAAAANFAWVNRSSMTFLTRQAFAKMFEATPDDLDMHVIYDVSHNIAKVEEHMYQGRPKTLLVHRKGSTRAFPPNHPLIPVDYQFTGQPVLIGGTMGTCSYVLTGTDKGLEETFGSTCHGAGRAQSRNKSRKTLEYTEVLAALEEKGISIRVASPKLVMEEAPESYKDVTQVVDTCHEAGISRKAIKLRPIAVVKG 510
            MANGSVVRTFEEEMGFLHRETPTRFRIDKGCVPNMKVEGAFYVNTKLEELMFAELKQHTTAGGVGGFLPAVKQIANVAALPGIVGRSIGLPDVHSGYGFAIGNVAAFAVADTEAIVSPGGVGFDINCGVRLIRTNLTEEDVRGKREKLTQSLFDHIPVGVGSKGVIPTSAADLEAALEMGMDWSLREGYAWAEDNEHCEENGRMRQADPTKVGMRAKKRGLPQLGTLGAGNHYAEIQVVEEIHDPRAAARMGIDQIGQVCVMIHSGSRGLGHQVATDALVEMERAMARDGILTNDRQLACARIQSPEGQNYLAAMAAAANFAWVNRSSMTFLTRQAFAKMFEATPDDLDMHVIYDVSHNIAKVEEHMYQGRPKTLLVHRKGSTRAFPPNHPLIPVDYQFTGQPVLIGGTMGTCSYVLTGTDKGLEETFGSTCHGAGRAQSRNKSRKTLEYTEVLAALEEKGISIRVASPKLVMEEAPESYKDVTQVVDTCHEAGISRKAIKLRPIAVVKG
Sbjct:    1 MANGSVVRTFEEEMGFLHRETPTRFRIDKGCVPNMKVEGAFYVNTKLEELMFAELKQHTTAGGVGGFLPAVKQIANVAALPGIVGRSIGLPDVHSGYGFAIGNVAAFAVADTEAIVSPGGVGFDINCGVRLIRTNLTEEDVRGKREKLTQSLFDHIPVGVGSKGVIPTSAADLEAALEMGMDWSLREGYAWAEDNEHCEENGRMRQADPTKVGMRAKKRGLPQLGTLGAGNHYAEIQVVEEIHDPRAAARMGIDQIGQVCVMIHSGSRGLGHQVATDALVEMERAMARDGILTNDRQLACARIQSPEGQNYLAAMAAAANFAWVNRSSMTFLTRQAFAKMFEATPDDLDMHVIYDVSHNIAKVEEHMYQGRPKTLLVHRKGSTRAFPPNHPLIPVDYQFTGQPVLIGGTMGTCSYVLTGTDKGLEETFGSTCHGAGRAQSRNKSRKTLEYTEVLAALEEKGISIRVASPKLVMEEAPESYKDVTQVVDTCHEAGISRKAIKLRPIAVVKG 510          

BLAST of EWM25480.1 vs. NCBI_GenBank
Match: gi|255079850|ref|XP_002503505.1| (predicted protein [Micromonas commoda] >gi|327488439|sp|C1E9Y5.1|RTCB_MICSR RecName: Full=tRNA-splicing ligase RtcB homolog >gi|226518772|gb|ACO64763.1| predicted protein [Micromonas commoda])

HSP 1 Score: 846.269 bits (2185), Expect = 0.000e+0
Identity = 399/503 (79.32%), Postives = 445/503 (88.47%), Query Frame = 0

Query:    8 RTFEEEMGFLHRETPTRFRIDKGCVPNMKVEGAFYVNTKLEELMFAELKQHTTAGGVGGFLPAVKQIANVAALPGIVGRSIGLPDVHSGYGFAIGNVAAFAVADTEAIVSPGGVGFDINCGVRLIRTNLTEEDVRGKREKLTQSLFDHIPVGVGSKGVIPTSAADLEAALEMGMDWSLREGYAWAEDNEHCEENGRMRQADPTKVGMRAKKRGLPQLGTLGAGNHYAEIQVVEEIHDPRAAARMGIDQIGQVCVMIHSGSRGLGHQVATDALVEMERAMARDGILTNDRQLACARIQSPEGQNYLAAMAAAANFAWVNRSSMTFLTRQAFAKMFEATPDDLDMHVIYDVSHNIAKVEEHMYQGRPKTLLVHRKGSTRAFPPNHPLIPVDYQFTGQPVLIGGTMGTCSYVLTGTDKGLEETFGSTCHGAGRAQSRNKSRKTLEYTEVLAALEEKGISIRVASPKLVMEEAPESYKDVTQVVDTCHEAGISRKAIKLRPIAVVKG 510
            RT+ +EM FL R TPT++R+ +G VPNM+V G FYVN  LE LMF EL+QH   G VGGFLPAVKQ+ANVA LPGIV +SI LPDVHSGYGFAIGNVAAF ++D  A+VSPGGVGFDINCGVR++RTNL E DV   +EKL QSLFDHIPVGVGS+G+IPTS A LEAALEMGMDWSLREGYAWAED EHCEE GRM  ADP KV  RAKKRGLPQ+GTLGAGNHYAEIQVV+EI D  AA +MGI+++GQV VMIHSGSRGLGHQVATDAL EMERAMARDGIL NDRQLACA+I SPEGQNYL+AM+ AAN+AWVNRSSMTFL RQAFAKMF+ TPDDLDMHV+YDVSHNIAK+EEH+  G  KTLLVHRKGSTRAFPP+HPLIPVDYQ+TGQPVLIGGTMGTCSY+LTGT+KG+EETFGSTCHGAGRA+SRN SR  L+YTEVL  L+ KGI+IRVASPKLVMEEAPESYKDVT+VV+TCH+AGIS+KA+KLRPIAVVKG
Sbjct:   11 RTYAQEMEFLERVTPTQWRVREGFVPNMRVPGVFYVNKHLETLMFDELRQHVDRGDVGGFLPAVKQLANVACLPGIVSKSIALPDVHSGYGFAIGNVAAFDMSDPNAVVSPGGVGFDINCGVRVVRTNLHERDVTDIKEKLAQSLFDHIPVGVGSQGIIPTSPAGLEAALEMGMDWSLREGYAWAEDKEHCEEYGRMLNADPNKVSARAKKRGLPQMGTLGAGNHYAEIQVVDEIFDKHAADKMGIERLGQVMVMIHSGSRGLGHQVATDALTEMERAMARDGILVNDRQLACAKISSPEGQNYLSAMSCAANYAWVNRSSMTFLCRQAFAKMFDQTPDDLDMHVVYDVSHNIAKIEEHVVDGELKTLLVHRKGSTRAFPPHHPLIPVDYQYTGQPVLIGGTMGTCSYILTGTEKGMEETFGSTCHGAGRARSRNNSRNKLDYTEVLEKLKTKGIAIRVASPKLVMEEAPESYKDVTEVVNTCHDAGISKKAVKLRPIAVVKG 513          

BLAST of EWM25480.1 vs. NCBI_GenBank
Match: gi|1022759492|gb|KZS05822.1| (tRNA-splicing ligase RtcB [Daphnia magna])

HSP 1 Score: 843.958 bits (2179), Expect = 0.000e+0
Identity = 399/503 (79.32%), Postives = 443/503 (88.07%), Query Frame = 0

Query:    8 RTFEEEMGFLHRETPTRFRIDKGCVPNMKVEGAFYVNTKLEELMFAELKQHTTAGGVGGFLPAVKQIANVAALPGIVGRSIGLPDVHSGYGFAIGNVAAFAVADTEAIVSPGGVGFDINCGVRLIRTNLTEEDVRGKREKLTQSLFDHIPVGVGSKGVIPTSAADLEAALEMGMDWSLREGYAWAEDNEHCEENGRMRQADPTKVGMRAKKRGLPQLGTLGAGNHYAEIQVVEEIHDPRAAARMGIDQIGQVCVMIHSGSRGLGHQVATDALVEMERAMARDGILTNDRQLACARIQSPEGQNYLAAMAAAANFAWVNRSSMTFLTRQAFAKMFEATPDDLDMHVIYDVSHNIAKVEEHMYQGRPKTLLVHRKGSTRAFPPNHPLIPVDYQFTGQPVLIGGTMGTCSYVLTGTDKGLEETFGSTCHGAGRAQSRNKSRKTLEYTEVLAALEEKGISIRVASPKLVMEEAPESYKDVTQVVDTCHEAGISRKAIKLRPIAVVKG 510
            R++EEE+ F+ R +P  +RI KG VPNMKVEG FYVN  LE LMF ELK  +  G VGGFLP +KQIANVAALPGIVGRSIGLPDVHSGYGFAIGNVAAF + D  +IVSPGGVGFDINCGVRL+RTNLTE+DV+  +E+LTQS+FDHIPVGVGSKGVIP +A DLE ALEMG+DWSLREGYAWAED EHCEE GRM  ADP+KV +RAKKRGLPQLGTLGAGNHYAEIQVV+EI+D  AA +MGI+  GQVC+MIHSGSRG GHQVATDALV MERAM RD I+TNDRQLACARI S EGQ+YL AM+AAANFAWVNRSSMTFL RQAFAK F +TPDDLDMH+IYDVSHNIAKVE+H   G+ KTLLVHRKGSTRAFPP+HPLIPVDYQ TGQPVLIGGTMGTCSY+LTGT+KG+EETFGSTCHGAGRA SR KSR+ L+YTEVL  L+E+GISIRVASPKLVMEEAPESYK+VT VVDTCH AGIS+K +KLRPIAV+KG
Sbjct:    3 RSYEEELKFIERLSPYSWRIKKGFVPNMKVEGIFYVNNNLENLMFEELKASSAQGLVGGFLPGMKQIANVAALPGIVGRSIGLPDVHSGYGFAIGNVAAFDMDDPNSIVSPGGVGFDINCGVRLLRTNLTEKDVQPVKEQLTQSMFDHIPVGVGSKGVIPINAQDLEEALEMGVDWSLREGYAWAEDKEHCEEFGRMLNADPSKVSLRAKKRGLPQLGTLGAGNHYAEIQVVDEIYDKWAATKMGIEHKGQVCIMIHSGSRGFGHQVATDALVTMERAMKRDNIVTNDRQLACARISSTEGQDYLKAMSAAANFAWVNRSSMTFLCRQAFAKQFNSTPDDLDMHLIYDVSHNIAKVEQHTVNGKLKTLLVHRKGSTRAFPPHHPLIPVDYQLTGQPVLIGGTMGTCSYILTGTEKGMEETFGSTCHGAGRALSRAKSRRNLDYTEVLDRLKEQGISIRVASPKLVMEEAPESYKNVTDVVDTCHAAGISKKTVKLRPIAVIKG 505          

BLAST of EWM25480.1 vs. NCBI_GenBank
Match: gi|340371191|ref|XP_003384129.1| (PREDICTED: tRNA-splicing ligase RtcB homolog [Amphimedon queenslandica])

HSP 1 Score: 840.106 bits (2169), Expect = 0.000e+0
Identity = 396/506 (78.26%), Postives = 446/506 (88.14%), Query Frame = 0

Query:    5 SVVRTFEEEMGFLHRETPTRFRIDKGCVPNMKVEGAFYVNTKLEELMFAELKQHTTAGGVGGFLPAVKQIANVAALPGIVGRSIGLPDVHSGYGFAIGNVAAFAVADTEAIVSPGGVGFDINCGVRLIRTNLTEEDVRGKREKLTQSLFDHIPVGVGSKGVIPTSAADLEAALEMGMDWSLREGYAWAEDNEHCEENGRMRQADPTKVGMRAKKRGLPQLGTLGAGNHYAEIQVVEEIHDPRAAARMGIDQIGQVCVMIHSGSRGLGHQVATDALVEMERAMARDGILTNDRQLACARIQSPEGQNYLAAMAAAANFAWVNRSSMTFLTRQAFAKMFEATPDDLDMHVIYDVSHNIAKVEEHMYQGRPKTLLVHRKGSTRAFPPNHPLIPVDYQFTGQPVLIGGTMGTCSYVLTGTDKGLEETFGSTCHGAGRAQSRNKSRKTLEYTEVLAALEEKGISIRVASPKLVMEEAPESYKDVTQVVDTCHEAGISRKAIKLRPIAVVKG 510
            S VRT++EE  +L + +   +RI KG VPNMKVEG FYVN  L++LMF EL+ H ++GGVGGFLPAVKQI NVAALPGIVG+SIGLPDVHSGYGFAIGN+AAF ++D EA+VSPGGVGFDINCGVRL+RTNL ++D+   +E+L QSLFDHIPVGVGSKGVIP +A DLE+ALEMGMDW+LREGY WAED EHCEE GRM QADP+KV  RAKKRGLPQLGTLGAGNHYAEIQVVEEI D  AAA+MGI++  QVCVMIHSGSRGLGHQVATDALVEME+AM RD I  NDRQLACA+I+S EG+NYL  MAAAAN+AWVNRSSMTFLTRQAFAKMF++TPDDLDMH+IYDVSHNIAKVE+H+  GR KTLLVHRKGSTRAFPP+HPLIPVDYQ TGQPVLIGGTMGTCSYVLTGTDKG  ETFGSTCHGAGRA SR KSR+TL Y EVL +L ++GI+IRVASPKLVMEEAP+SYK+VT VV+TCH AGIS KA KL+PIAV+KG
Sbjct:    3 SAVRTYDEECSYLEKISNCSYRIKKGFVPNMKVEGLFYVNDHLKDLMFDELRHHCSSGGVGGFLPAVKQIGNVAALPGIVGKSIGLPDVHSGYGFAIGNMAAFDMSDPEAVVSPGGVGFDINCGVRLLRTNLFKKDIEPVKEQLAQSLFDHIPVGVGSKGVIPMTARDLESALEMGMDWTLREGYGWAEDKEHCEEYGRMLQADPSKVSQRAKKRGLPQLGTLGAGNHYAEIQVVEEIFDKHAAAKMGIEEKEQVCVMIHSGSRGLGHQVATDALVEMEKAMKRDNIEVNDRQLACAKIKSKEGENYLKGMAAAANYAWVNRSSMTFLTRQAFAKMFKSTPDDLDMHIIYDVSHNIAKVEQHIVSGREKTLLVHRKGSTRAFPPHHPLIPVDYQLTGQPVLIGGTMGTCSYVLTGTDKGFTETFGSTCHGAGRALSRAKSRRTLGYEEVLESLAKQGIAIRVASPKLVMEEAPQSYKNVTDVVETCHSAGISNKAFKLKPIAVIKG 508          

BLAST of EWM25480.1 vs. NCBI_GenBank
Match: gi|303272537|ref|XP_003055630.1| (predicted protein [Micromonas pusilla CCMP1545] >gi|327488438|sp|C1MI97.1|RTCB_MICPC RecName: Full=tRNA-splicing ligase RtcB homolog >gi|226463604|gb|EEH60882.1| predicted protein [Micromonas pusilla CCMP1545])

HSP 1 Score: 840.106 bits (2169), Expect = 0.000e+0
Identity = 392/503 (77.93%), Postives = 444/503 (88.27%), Query Frame = 0

Query:    8 RTFEEEMGFLHRETPTRFRIDKGCVPNMKVEGAFYVNTKLEELMFAELKQHTTAGGVGGFLPAVKQIANVAALPGIVGRSIGLPDVHSGYGFAIGNVAAFAVADTEAIVSPGGVGFDINCGVRLIRTNLTEEDVRGKREKLTQSLFDHIPVGVGSKGVIPTSAADLEAALEMGMDWSLREGYAWAEDNEHCEENGRMRQADPTKVGMRAKKRGLPQLGTLGAGNHYAEIQVVEEIHDPRAAARMGIDQIGQVCVMIHSGSRGLGHQVATDALVEMERAMARDGILTNDRQLACARIQSPEGQNYLAAMAAAANFAWVNRSSMTFLTRQAFAKMFEATPDDLDMHVIYDVSHNIAKVEEHMYQGRPKTLLVHRKGSTRAFPPNHPLIPVDYQFTGQPVLIGGTMGTCSYVLTGTDKGLEETFGSTCHGAGRAQSRNKSRKTLEYTEVLAALEEKGISIRVASPKLVMEEAPESYKDVTQVVDTCHEAGISRKAIKLRPIAVVKG 510
            RT+++EM FL R +  ++ +++G VPNM+V G FYVN  LE LMF EL+QH  +GG GGFLPAVKQ+ANVA LPGIVG+SI +PDVHSGYGF IGNVAAF ++D  A+VSPGGVGFDINCGVR++RTNL E DV   +EKL Q+LFDHIPVGVGS+G+IPTSAA LE+ALEMGMDWSLREGYAWAED EHCEE GRM  ADP KV  RAKKRGLPQ+GTLGAGNHYAEIQVV+EI D  AA +MG+D +GQV VMIHSGSRGLGHQVATDAL EMERAMARDGI TNDRQLACARI S EGQNYL+AM+ AAN+AWVNRSSMTFL RQAFAKMF++TPDDLDMHV+YDVSHNIAK+EEHM  G+ KTLLVHRKGSTRAFPP+HPLIPVDYQ+TGQPV+IGGTMGTCSY+LTGT KG++ETFGSTCHGAGRA+SRN SR  L+Y EVL  L+ KGISIRVASPKLVMEEAPESYKDVT+VV+TCH+AGIS+KA+KLRPIAVVKG
Sbjct:    5 RTYQQEMAFLSRVSANQWIVNEGFVPNMRVPGTFYVNKHLETLMFEELQQHVDSGGHGGFLPAVKQLANVACLPGIVGKSIAMPDVHSGYGFCIGNVAAFDMSDPAAVVSPGGVGFDINCGVRVVRTNLMESDVADVKEKLAQALFDHIPVGVGSQGIIPTSAAGLESALEMGMDWSLREGYAWAEDKEHCEEYGRMLTADPNKVSARAKKRGLPQMGTLGAGNHYAEIQVVDEIFDAHAAEKMGVDAVGQVMVMIHSGSRGLGHQVATDALTEMERAMARDGIETNDRQLACARINSTEGQNYLSAMSCAANYAWVNRSSMTFLCRQAFAKMFDSTPDDLDMHVVYDVSHNIAKIEEHMVDGKLKTLLVHRKGSTRAFPPHHPLIPVDYQYTGQPVMIGGTMGTCSYILTGTQKGMDETFGSTCHGAGRARSRNNSRNKLDYAEVLENLKTKGISIRVASPKLVMEEAPESYKDVTEVVNTCHDAGISKKAVKLRPIAVVKG 507          

BLAST of EWM25480.1 vs. NCBI_GenBank
Match: gi|1101400003|ref|XP_018911068.1| (PREDICTED: tRNA-splicing ligase RtcB homolog isoform X1 [Bemisia tabaci])

HSP 1 Score: 832.402 bits (2149), Expect = 0.000e+0
Identity = 392/505 (77.62%), Postives = 440/505 (87.13%), Query Frame = 0

Query:    6 VVRTFEEEMGFLHRETPTRFRIDKGCVPNMKVEGAFYVNTKLEELMFAELKQHTTAGGVGGFLPAVKQIANVAALPGIVGRSIGLPDVHSGYGFAIGNVAAFAVADTEAIVSPGGVGFDINCGVRLIRTNLTEEDVRGKREKLTQSLFDHIPVGVGSKGVIPTSAADLEAALEMGMDWSLREGYAWAEDNEHCEENGRMRQADPTKVGMRAKKRGLPQLGTLGAGNHYAEIQVVEEIHDPRAAARMGIDQIGQVCVMIHSGSRGLGHQVATDALVEMERAMARDGILTNDRQLACARIQSPEGQNYLAAMAAAANFAWVNRSSMTFLTRQAFAKMFEATPDDLDMHVIYDVSHNIAKVEEHMYQGRPKTLLVHRKGSTRAFPPNHPLIPVDYQFTGQPVLIGGTMGTCSYVLTGTDKGLEETFGSTCHGAGRAQSRNKSRKTLEYTEVLAALEEKGISIRVASPKLVMEEAPESYKDVTQVVDTCHEAGISRKAIKLRPIAVVKG 510
            VVR + EE+ +L + TP  +RI KG  PNM VEG FYVN  LE+LMF EL+     G VGGFLP +KQIANVAALPGIVG+S+GLPDVHSGYGFAIGN+AAF   D +++VSPGGVGFDINCGVRL+RTNL E+DV   +E+L QS+FDHIPVGVGSKG+IP +A DLE ALEMGMDWSLREGY WAED EHCEE GRM  ADP+KV MRAKKRGLPQLGTLGAGNHYAEIQVVEEI+D  AA +MGI++ GQ+CVMIHSGSRG GHQVATDALV+ME+AM RD I TNDRQLACARI S EGQ+YL +M+AAANFAWVNRSSMTFL+RQAFAK F  TPDDLDMHVIYDVSHNIAK EEHM  G+P+TLLVHRKGSTRAFPP+HPLIPVDYQ TGQPVLIGGTMGTCSYVLTGT++G++ETFGSTCHGAGRA SR KSR+ L+YTEVL  L+E+GISIRVASPKLVMEEAPESYK+VT VVDTCH AGIS+K+IKLRPIAV+KG
Sbjct:    2 VVRQYNEELKYLEKLTPYSWRIKKGFQPNMNVEGIFYVNNHLEKLMFDELRNACRPGMVGGFLPGMKQIANVAALPGIVGKSVGLPDVHSGYGFAIGNMAAFDANDPKSVVSPGGVGFDINCGVRLLRTNLFEKDVLPVKEQLAQSMFDHIPVGVGSKGIIPMNARDLEEALEMGMDWSLREGYIWAEDKEHCEEYGRMLNADPSKVSMRAKKRGLPQLGTLGAGNHYAEIQVVEEIYDKWAADKMGIEEKGQICVMIHSGSRGFGHQVATDALVQMEKAMKRDNIETNDRQLACARIHSKEGQDYLKSMSAAANFAWVNRSSMTFLSRQAFAKQFNTTPDDLDMHVIYDVSHNIAKTEEHMVDGKPRTLLVHRKGSTRAFPPHHPLIPVDYQLTGQPVLIGGTMGTCSYVLTGTEQGMQETFGSTCHGAGRALSRAKSRRNLDYTEVLRKLDEQGISIRVASPKLVMEEAPESYKNVTDVVDTCHAAGISKKSIKLRPIAVIKG 506          

BLAST of EWM25480.1 vs. NCBI_GenBank
Match: gi|470308521|ref|XP_004363837.1| (hypothetical protein CAOG_02998 [Capsaspora owczarzaki ATCC 30864])

HSP 1 Score: 832.017 bits (2148), Expect = 0.000e+0
Identity = 397/509 (78.00%), Postives = 446/509 (87.62%), Query Frame = 0

Query:    2 ANGSVVRTFEEEMGFLHRETPTRFRIDKGCVPNMKVEGAFYVNTKLEELMFAELKQHTTAGGVGGFLPAVKQIANVAALPGIVGRSIGLPDVHSGYGFAIGNVAAFAVADTEAIVSPGGVGFDINCGVRLIRTNLTEEDVRGKREKLTQSLFDHIPVGVGSKGVIPTSAADLEAALEMGMDWSLREGYAWAEDNEHCEENGRMRQADPTKVGMRAKKRGLPQLGTLGAGNHYAEIQVVEEIHDPRAAARMGIDQIGQVCVMIHSGSRGLGHQVATDALVEMERAMARDGILTNDRQLACARIQSPEGQNYLAAMAAAANFAWVNRSSMTFLTRQAFAKMFEATPDDLDMHVIYDVSHNIAKVEEHMYQGRPKTLLVHRKGSTRAFPPNHPLIPVDYQFTGQPVLIGGTMGTCSYVLTGTDKGLEETFGSTCHGAGRAQSRNKSRKTLEYTEVLAALEEKGISIRVASPKLVMEEAPESYKDVTQVVDTCHEAGISRKAIKLRPIAVVKG 510
            A   ++R++E+E  ++ R +PT+FRI KG V NMKV+G FYVN  L+ELMF EL+Q + + GVGGFLPAVKQIANVAALPGIVGRSIGLPD+HSGYGFAIGN+AAF + D EAIVSPGGVGFDINCGVRLIRTNLTE++V   +E+L Q+LFDHIPVGVGSKGVIP +  +LE ALEMGMDWSLREGYAWAED EHCEE GRM QA+P  V  RAKKRGLPQLGTLGAGNHY EIQVV+EI+D  AA +MGIDQ GQVC+M+HSGSRGLGHQVATD+LV ME+AM RDGI  NDRQLACARIQS EGQ+YL  MAAAAN+AWVNRSSMTFLTRQAFAK+F +TPDDLDMHVIYDVSHNIAKVEEHM      TLLVHRKGSTRAFPP+HPLIPVDYQ  GQPVLIGGTMGTCS+VLTGT+KG+ ETFGSTCHGAGRAQSRNKSR+TL+Y +VL +L +KGISIRVASPKLV EEAPESYKDV  VV+TCH+AGIS+KAIKLRPIAV+KG
Sbjct:   11 AASGILRSYEDECKYIERVSPTQFRIKKGFVHNMKVDGCFYVNQHLQELMFDELQQFSDSRGVGGFLPAVKQIANVAALPGIVGRSIGLPDIHSGYGFAIGNMAAFDMNDPEAIVSPGGVGFDINCGVRLIRTNLTEKEVGPVKEELAQALFDHIPVGVGSKGVIPMTMHNLEEALEMGMDWSLREGYAWAEDKEHCEEYGRMLQANPAMVSQRAKKRGLPQLGTLGAGNHYCEIQVVDEIYDAHAARKMGIDQKGQVCIMVHSGSRGLGHQVATDSLVSMEKAMRRDGIEVNDRQLACARIQSKEGQHYLQGMAAAANYAWVNRSSMTFLTRQAFAKIFNSTPDDLDMHVIYDVSHNIAKVEEHMVD---VTLLVHRKGSTRAFPPHHPLIPVDYQLIGQPVLIGGTMGTCSFVLTGTEKGMLETFGSTCHGAGRAQSRNKSRRTLDYQDVLDSLAQKGISIRVASPKLVTEEAPESYKDVIDVVETCHQAGISKKAIKLRPIAVIKG 516          

BLAST of EWM25480.1 vs. NCBI_GenBank
Match: gi|321455578|gb|EFX66707.1| (hypothetical protein DAPPUDRAFT_302483 [Daphnia pulex])

HSP 1 Score: 831.247 bits (2146), Expect = 0.000e+0
Identity = 394/505 (78.02%), Postives = 441/505 (87.33%), Query Frame = 0

Query:    6 VVRTFEEEMGFLHRETPTRFRIDKGCVPNMKVEGAFYVNTKLEELMFAELKQHTTAGGVGGFLPAVKQIANVAALPGIVGRSIGLPDVHSGYGFAIGNVAAFAVADTEAIVSPGGVGFDINCGVRLIRTNLTEEDVRGKREKLTQSLFDHIPVGVGSKGVIPTSAADLEAALEMGMDWSLREGYAWAEDNEHCEENGRMRQADPTKVGMRAKKRGLPQLGTLGAGNHYAEIQVVEEIHDPRAAARMGIDQIGQVCVMIHSGSRGLGHQVATDALVEMERAMARDGILTNDRQLACARIQSPEGQNYLAAMAAAANFAWVNRSSMTFLTRQAFAKMFEATPDDLDMHVIYDVSHNIAKVEEHMYQGRPKTLLVHRKGSTRAFPPNHPLIPVDYQFTGQPVLIGGTMGTCSYVLTGTDKGLEETFGSTCHGAGRAQSRNKSRKTLEYTEVLAALEEKGISIRVASPKLVMEEAPESYKDVTQVVDTCHEAGISRKAIKLRPIAVVKG 510
            +VRT+++EM FL R +P  +RI KG VPNM VEG FYVN  LE+LMF EL+     G VGGFLP +KQIANVAALPGIVGRSIGLPDVHSGYGFAIGNVAAF + D  +IVSPGGVGFDINCGVRL+RTNLTE+DV+  +E+LTQS+FDHIPVGVGSKGVIP +A DLE ALEMG+DWSLREGYAWAED EHCEE GRM  ADP+KV +RAKKRGLPQLGTLGAGNHYAEIQVV+EI+D  AA +MGI+  GQVC+MIHSGSRG GHQVATDAL  M++AM RD I+TNDRQLACARI S EGQ+YL  MAAAANFAWVNR+SMTFL RQAFAK F +TPDDLDMHVIYDVSHNIAKVE+H   G+ KTLLVHRKGSTRAFPP+HPLIPVDYQ TGQPVLIGGTMGTCSY+LTGT++G+ ETFGSTCHGAGRA SR KSR+ L+YTEVL  L+E+GISIRVASPKLVMEEAPESYK+VT VVDTCH AGIS+K +KLRPIAV+KG
Sbjct:    1 MVRTYDDEMKFLERLSPCSWRIKKGFVPNMNVEGIFYVNNNLEDLMFDELRNACAQGLVGGFLPGMKQIANVAALPGIVGRSIGLPDVHSGYGFAIGNVAAFDMDDPNSIVSPGGVGFDINCGVRLLRTNLTEKDVQPFKEQLTQSMFDHIPVGVGSKGVIPITAQDLEEALEMGVDWSLREGYAWAEDKEHCEEFGRMLNADPSKVSLRAKKRGLPQLGTLGAGNHYAEIQVVDEIYDKWAANKMGIEHKGQVCIMIHSGSRGFGHQVATDALNSMDKAMKRDKIVTNDRQLACARISSTEGQDYLKGMAAAANFAWVNRASMTFLCRQAFAKQFNSTPDDLDMHVIYDVSHNIAKVEQHTVDGKLKTLLVHRKGSTRAFPPHHPLIPVDYQLTGQPVLIGGTMGTCSYILTGTEQGMVETFGSTCHGAGRALSRAKSRRNLDYTEVLERLKEQGISIRVASPKLVMEEAPESYKNVTDVVDTCHAAGISKKTVKLRPIAVIKG 505          

BLAST of EWM25480.1 vs. NCBI_GenBank
Match: gi|568250734|gb|ETN60385.1| (tRNA-splicing ligase RtcB like protein 1 [Anopheles darlingi])

HSP 1 Score: 830.091 bits (2143), Expect = 0.000e+0
Identity = 390/505 (77.23%), Postives = 436/505 (86.34%), Query Frame = 0

Query:    6 VVRTFEEEMGFLHRETPTRFRIDKGCVPNMKVEGAFYVNTKLEELMFAELKQHTTAGGVGGFLPAVKQIANVAALPGIVGRSIGLPDVHSGYGFAIGNVAAFAVADTEAIVSPGGVGFDINCGVRLIRTNLTEEDVRGKREKLTQSLFDHIPVGVGSKGVIPTSAADLEAALEMGMDWSLREGYAWAEDNEHCEENGRMRQADPTKVGMRAKKRGLPQLGTLGAGNHYAEIQVVEEIHDPRAAARMGIDQIGQVCVMIHSGSRGLGHQVATDALVEMERAMARDGILTNDRQLACARIQSPEGQNYLAAMAAAANFAWVNRSSMTFLTRQAFAKMFEATPDDLDMHVIYDVSHNIAKVEEHMYQGRPKTLLVHRKGSTRAFPPNHPLIPVDYQFTGQPVLIGGTMGTCSYVLTGTDKGLEETFGSTCHGAGRAQSRNKSRKTLEYTEVLAALEEKGISIRVASPKLVMEEAPESYKDVTQVVDTCHEAGISRKAIKLRPIAVVKG 510
            VVR + EEM ++ R +P  F I KG  PNM V+G FY N++LE+LMF EL+     G  GGFLP VKQIANVAALPGIVGRS+GLPD+HSGYGFAIGN+AAF + D  +IVSPGGVGFDINCGVRL+RTNL E+DV   +E+L QSLFDHIPVGVGSKG+IP +A DLE ALEMGMDWSLREGY WAED EHCEE GRM  ADP+KV +RAKKRGLPQLGTLGAGNHYAEIQVVEEI+D  AA++MGI+++GQ+CVMIHSGSRG GHQVATDALVEME+AM RD I TNDRQLACARI SPEGQNYL AM+AAANFAWVNRSSMTFLTRQAFAK F  TPDDLDMHVIYDVSHN+AK+EEHM  GRPK LLVHRKGSTRAFPP+HPLIPVDYQ TGQPVL+GG+MGTCS+VLTGT+KG+ ETFGSTCHGAGR+ SR KSR+ L+Y +VL  LEEKGISIRVASPKLV EEAP+SYKDV  VV TCH+ GIS KAIKLRPIAV+KG
Sbjct:    2 VVREYNEEMKYIERLSPNSFLIKKGFQPNMNVDGVFYANSRLEKLMFEELRNACRPGMSGGFLPGVKQIANVAALPGIVGRSVGLPDIHSGYGFAIGNMAAFDMNDPTSIVSPGGVGFDINCGVRLLRTNLFEKDVTPVKEQLAQSLFDHIPVGVGSKGIIPMNAHDLEEALEMGMDWSLREGYVWAEDKEHCEEYGRMLNADPSKVSLRAKKRGLPQLGTLGAGNHYAEIQVVEEIYDKYAASKMGIEELGQICVMIHSGSRGFGHQVATDALVEMEKAMKRDKIETNDRQLACARINSPEGQNYLKAMSAAANFAWVNRSSMTFLTRQAFAKQFNTTPDDLDMHVIYDVSHNVAKMEEHMVDGRPKQLLVHRKGSTRAFPPHHPLIPVDYQLTGQPVLVGGSMGTCSFVLTGTEKGMVETFGSTCHGAGRSLSRAKSRRQLDYKDVLRDLEEKGISIRVASPKLVQEEAPDSYKDVRDVVQTCHDVGISNKAIKLRPIAVIKG 506          

BLAST of EWM25480.1 vs. NCBI_GenBank
Match: gi|1080061659|ref|XP_018572837.1| (PREDICTED: tRNA-splicing ligase RtcB homolog [Anoplophora glabripennis])

HSP 1 Score: 829.321 bits (2141), Expect = 0.000e+0
Identity = 395/505 (78.22%), Postives = 439/505 (86.93%), Query Frame = 0

Query:    6 VVRTFEEEMGFLHRETPTRFRIDKGCVPNMKVEGAFYVNTKLEELMFAELKQHTTAGGVGGFLPAVKQIANVAALPGIVGRSIGLPDVHSGYGFAIGNVAAFAVADTEAIVSPGGVGFDINCGVRLIRTNLTEEDVRGKREKLTQSLFDHIPVGVGSKGVIPTSAADLEAALEMGMDWSLREGYAWAEDNEHCEENGRMRQADPTKVGMRAKKRGLPQLGTLGAGNHYAEIQVVEEIHDPRAAARMGIDQIGQVCVMIHSGSRGLGHQVATDALVEMERAMARDGILTNDRQLACARIQSPEGQNYLAAMAAAANFAWVNRSSMTFLTRQAFAKMFEATPDDLDMHVIYDVSHNIAKVEEHMYQGRPKTLLVHRKGSTRAFPPNHPLIPVDYQFTGQPVLIGGTMGTCSYVLTGTDKGLEETFGSTCHGAGRAQSRNKSRKTLEYTEVLAALEEKGISIRVASPKLVMEEAPESYKDVTQVVDTCHEAGISRKAIKLRPIAVVKG 510
            VVR ++EE+ +L + +P  +RI KG  PNMKVEG FYVN +LE+LM  EL+     G VGGFLP VKQIANVAALPGIVGRS+GLPDVHSGYGFAIGN+AAF + + ++IVSPGGVGFDINCGVRL+RTNL E+DV   +E+L QSLFDHIPVGVGSKG+IP +A DLE ALEMGMDWSLREGY WAED EHCEE GRM  ADP KV MRAKKRGLPQLGTLGAGNHYAEIQVVEEI+D  AA +MGI++IGQVCVMIHSGSRG GHQVATDALV+ME+AM RD I TNDRQLACARI S EGQ+YL +MAAAANFAWVNRSSMTFL+RQAFAK F  +PDDLDMHVIYDVSHNIAKVEEH+  G+P TLLVHRKGSTRAFPP+HPLIPVDYQ TGQPVLIGGTMGTCSYVLTGT++G+ ETFGSTCHGAGRA SR KSR+ L+YT+VL  LE  GISIRVASPKLVMEEAPESYK+VT VVDTCH AGIS+K IKLRPIAV+KG
Sbjct:    2 VVRQYQEELQYLEKLSPYCWRIKKGFQPNMKVEGVFYVNQRLEKLMLDELRNACRPGMVGGFLPGVKQIANVAALPGIVGRSVGLPDVHSGYGFAIGNMAAFDINNPDSIVSPGGVGFDINCGVRLLRTNLFEQDVLPIKEQLAQSLFDHIPVGVGSKGIIPMNAKDLEEALEMGMDWSLREGYIWAEDKEHCEEYGRMLNADPAKVSMRAKKRGLPQLGTLGAGNHYAEIQVVEEIYDKWAACKMGIEEIGQVCVMIHSGSRGFGHQVATDALVQMEKAMKRDNIETNDRQLACARINSVEGQDYLKSMAAAANFAWVNRSSMTFLSRQAFAKQFHMSPDDLDMHVIYDVSHNIAKVEEHIVDGKPTTLLVHRKGSTRAFPPHHPLIPVDYQLTGQPVLIGGTMGTCSYVLTGTEQGMLETFGSTCHGAGRALSRAKSRRNLDYTDVLRKLEYMGISIRVASPKLVMEEAPESYKNVTDVVDTCHAAGISKKCIKLRPIAVIKG 506