EWM27715.1, cds2922 (CDS) Nannochloropsis gaditana

BLAST of EWM27715.1 vs. NCBI_GenBank
Match: gi|585109988|gb|EWM27715.1| (aaa family cdc48 subfamily [Nannochloropsis gaditana])

HSP 1 Score: 1159.05 bits (2997), Expect = 0.000e+0
Identity = 575/575 (100.00%), Postives = 575/575 (100.00%), Query Frame = 0

Query:    1 MGLGQFPPHSDKKCQHRRSAIEADLPGLEEDIDLLLRLIDGFSRNGGGSRDQSFCQAVRGALLEGGPGAGKTALAKSLARHGGLPSYYCSCPSLFSRDRGDAERGLQEIFQTCIQKAACSEKESCLLVLDDLDCIGKDRKKARHVSDLGILAMLLECLDHLRGAHVFVLAIAGDASLIDVGLLQPGRLEITLKLSSLKQEQREKILANLLGKLNTKSPRAVEESSIQYSGRLLDSLACRTRGFNGADLAYLCSSALTSALRRSQSSDHVCIEEEDWEVAMEGAHPIDIPWLFVEGARLSDLAGMDEIIQTVSETILQPLLQPEALRAMGVPRASFGVLLYGPSGTGKTTLGLALAAAAQSHASFLAVECSELVNKVVGESERAVARLFQVARHRAPCILFLDHVEAIAGRRGFDTSSEQTMDRMLSTLLMEMDGLTAGDCMHDRTGSDMVIVVAATNLLSMLDEAFLRPGRLDILIEMALPDKEARAAIFLLHFSRMPLRFDTAVRGLKSEKNSTFTPLHEFAETMADLSEGLSGADIQGLCQEAALLALREDLDRSYLLPRHVMEALDVKRRNT 575
            MGLGQFPPHSDKKCQHRRSAIEADLPGLEEDIDLLLRLIDGFSRNGGGSRDQSFCQAVRGALLEGGPGAGKTALAKSLARHGGLPSYYCSCPSLFSRDRGDAERGLQEIFQTCIQKAACSEKESCLLVLDDLDCIGKDRKKARHVSDLGILAMLLECLDHLRGAHVFVLAIAGDASLIDVGLLQPGRLEITLKLSSLKQEQREKILANLLGKLNTKSPRAVEESSIQYSGRLLDSLACRTRGFNGADLAYLCSSALTSALRRSQSSDHVCIEEEDWEVAMEGAHPIDIPWLFVEGARLSDLAGMDEIIQTVSETILQPLLQPEALRAMGVPRASFGVLLYGPSGTGKTTLGLALAAAAQSHASFLAVECSELVNKVVGESERAVARLFQVARHRAPCILFLDHVEAIAGRRGFDTSSEQTMDRMLSTLLMEMDGLTAGDCMHDRTGSDMVIVVAATNLLSMLDEAFLRPGRLDILIEMALPDKEARAAIFLLHFSRMPLRFDTAVRGLKSEKNSTFTPLHEFAETMADLSEGLSGADIQGLCQEAALLALREDLDRSYLLPRHVMEALDVKRRNT
Sbjct:    1 MGLGQFPPHSDKKCQHRRSAIEADLPGLEEDIDLLLRLIDGFSRNGGGSRDQSFCQAVRGALLEGGPGAGKTALAKSLARHGGLPSYYCSCPSLFSRDRGDAERGLQEIFQTCIQKAACSEKESCLLVLDDLDCIGKDRKKARHVSDLGILAMLLECLDHLRGAHVFVLAIAGDASLIDVGLLQPGRLEITLKLSSLKQEQREKILANLLGKLNTKSPRAVEESSIQYSGRLLDSLACRTRGFNGADLAYLCSSALTSALRRSQSSDHVCIEEEDWEVAMEGAHPIDIPWLFVEGARLSDLAGMDEIIQTVSETILQPLLQPEALRAMGVPRASFGVLLYGPSGTGKTTLGLALAAAAQSHASFLAVECSELVNKVVGESERAVARLFQVARHRAPCILFLDHVEAIAGRRGFDTSSEQTMDRMLSTLLMEMDGLTAGDCMHDRTGSDMVIVVAATNLLSMLDEAFLRPGRLDILIEMALPDKEARAAIFLLHFSRMPLRFDTAVRGLKSEKNSTFTPLHEFAETMADLSEGLSGADIQGLCQEAALLALREDLDRSYLLPRHVMEALDVKRRNT 575          

BLAST of EWM27715.1 vs. NCBI_GenBank
Match: gi|1101064418|gb|OIO25994.1| (AAA family ATPase [Candidatus Micrarchaeota archaeon CG1_02_55_41])

HSP 1 Score: 279.256 bits (713), Expect = 1.612e-81
Identity = 195/558 (34.95%), Postives = 291/558 (52.15%), Query Frame = 0

Query:   28 LEEDIDLLLRLIDGFSRNGGGSRDQSFCQAVRGALLEGGPGAGKTALAKSLARHGGLPSYYCSCPSLFSRDRGDAERGLQEIFQTCIQKAACSEKESCLLVLDDLDCIGKDRKKARHVSDLGILAMLLECLDHLRG-AHVFVLAIAGDASLIDVGLLQPGRLEITLKLSSLKQEQREKILANLLGKLNTKSPRAVEESSIQYSGRLLDSLACRTRGFNGADLAYLCSSALTSALRR---------SQSSDHVC----IEEEDWEVAMEGAHPIDIPWLFVE--GARLSDLAGMDEIIQTVSETILQPLLQPEALRAMGVPRASFGVLLYGPSGTGKTTLGLALAAAAQSHASFLAVECSELVNKVVGESERAVARLFQVARHRAPCILFLDHVEAIAGRRGFDTSSEQTMDRMLSTLLMEMDGLTAGDCMHDRTGSDMVIVVAATNLLSMLDEAFLRPGRLDILIEMALPDKEARAAIFLLHFSRMPLRFDTAVRGLKSEKNSTFTPLHEFAETMADLSEGLSGADIQGLCQEAALLALREDLDRSYLLPRHVMEALD 569
            + E ++L +R  + F R G         +  +G L+ G PG GKT LAK++A          S P + S+  G+AE  L+ IF+     A        ++ +D++D I   R++     +  I++ LL  +D L+    V V+A     + ID  L +PGR +  +++    +  R +IL     +++T+    +E+ SI       D LA  T GF GADL  LC  A   ALRR          +   H+     + ++D+E A++G  P  +  +FVE    +  D+ G+ E  + ++E I  PL  PEA   +G+ R   G+LLYGP GTGKT L  A A A +S A+F+AV   E+ NK VGESE++V  +F+ AR  APCI+F D ++A+A  RG D SS  T +R++++LL EMDG+         +    V+V+AATN   +LD A LRPGR D L+E+ LPD+EAR AIF +H   MPL  D  V                  E +   +E  SGADI  LC+EA L ALRE+++   + P+H   A +
Sbjct:  190 VREMVELPMRHPELFERLG--------IEPPKGVLIYGPPGCGKTLLAKAVANESDTHFITLSGPEIVSKFVGEAEEKLRNIFKEAEDNA------PAIIFIDEIDAIAPKREEVVGEVEKRIVSQLLVLMDGLKSRGQVVVIAATNRPNAIDPALRRPGRFDREIEIGVPDKRGRREIL-----EIHTRG-MPMEDVSI-------DDLAAHTHGFVGADLQSLCKEAAMLALRRVLPKMNLEDEEIPKHILEELKVNKKDFEDALKGIQPSALREVFVEIPNVKWEDVGGLKEAKREITEAIELPLKSPEAFTRLGI-RPVKGILLYGPPGTGKTLL--AKAVATESEANFIAVRGPEMFNKWVGESEKSVREVFRKARLAAPCIIFFDEIDALASARGLDESSHVT-ERVVNSLLAEMDGI---------SNQKNVVVIAATNRADILDPALLRPGRFDKLVEIPLPDEEARLAIFKVHTKGMPL--DKGVD----------------LEELVKKTEEYSGADISALCREAGLNALRENVNAKKVGPKHFENAFN 689          

HSP 2 Score: 160.229 bits (404), Expect = 3.216e-38
Identity = 97/286 (33.92%), Postives = 154/286 (53.85%), Query Frame = 0

Query:  293 VEGARLSDLAGMDEIIQTVSETILQPLLQPEALRAMGVPRASFGVLLYGPSGTGKTTLGLALAAAAQSHASFLAVECSELVNKVVGESERAVARLFQVARHRAPCILFLDHVEAIAGRRGFDTSSEQTMDRMLSTLLMEMDGLTAGDCMHDRTGSDMVIVVAATNLLSMLDEAFLRPGRLDILIEMALPDKEARAAIFLLHFSRMPLRFDTAVRGLKSEKNSTFTPLHEFAETMADLSEGLSGADIQGLCQEAALLALREDLDRSYL----LPRHVMEALDVKRRN 574
            +E     D+ G+ +++Q V E +  P+  PE    +G+     GVL+YGP G GKT L  A+A  + +H  F+ +   E+V+K VGE+E  +  +F+ A   AP I+F+D ++AIA +R  +    +   R++S LL+ MDGL +            V+V+AATN  + +D A  RPGR D  IE+ +PDK  R  I  +H   MP+  D ++                  + +A  + G  GAD+Q LC+EAA+LALR  L +  L    +P+H++E L V +++
Sbjct:  172 IETISYEDIGGLRDVVQKVREMVELPMRHPELFERLGI-EPPKGVLIYGPPGCGKTLLAKAVANESDTH--FITLSGPEIVSKFVGEAEEKLRNIFKEAEDNAPAIIFIDEIDAIAPKR--EEVVGEVEKRIVSQLLVLMDGLKS---------RGQVVVIAATNRPNAIDPALRRPGRFDREIEIGVPDKRGRREILEIHTRGMPME-DVSI------------------DDLAAHTHGFVGADLQSLCKEAAMLALRRVLPKMNLEDEEIPKHILEELKVNKKD 424          

BLAST of EWM27715.1 vs. NCBI_GenBank
Match: gi|499626557|ref|WP_011307291.1| (ATPase [Methanosarcina barkeri] >gi|72396972|gb|AAZ71245.1| cell division control protein 48 AAA family protein [Methanosarcina barkeri str. Fusaro] >gi|805401156|gb|AKB51676.1| Cell division protein FtsH [Methanosarcina barkeri str. Wiesmoor])

HSP 1 Score: 276.944 bits (707), Expect = 5.109e-80
Identity = 177/542 (32.66%), Postives = 286/542 (52.77%), Query Frame = 0

Query:   59 RGALLEGGPGAGKTALAKSLARHGGLPSYYCSCPSLFSRDRGDAERGLQEIFQTCIQKAACSEKESCLLVLDDLDCIGKDRKKARHVSDLGILAMLLECLDHL--RGAHVFVLAIAGDASLIDVGLLQPGRLEITLKLSSLKQEQREKIL--------------ANLLGKLNTKSPRA-VEESSIQYSGRLLDSLACRTRGFNGADLAYLCSSALTSALRRS-------------QSSDHVCIEEEDWEVAMEGAHPIDIPWLFVEGARLS--DLAGMDEIIQTVSETILQPLLQPEALRAMGVPRASFGVLLYGPSGTGKTTLGLALAAAAQSHASFLAVECSELVNKVVGESERAVARLFQVARHRAPCILFLDHVEAIAGRRGFDTSSEQTMDRMLSTLLMEMDGLTAGDCMHDRTGSDMVIVVAATNLLSMLDEAFLRPGRLDILIEMALPDKEARAAIFLLHFSRMPLRFDTAVRGLKSEKNSTFTPLHEFAETMADLSEGLSGADIQGLCQEAALLALREDLDRSYLLPRHVMEAL 568
            +G +L G PG GKT +AK++A   G   +Y + P +  +  G++E  L++IF+   Q A        ++ +D++D I   R+      +  ++A LL  LD +  RG  V V+        ID  L +PGR +  + +     + R +IL                 +GK+ T++  A +E    + + + L  LA RT+GF GADL  L   A    LR +             +  + + + + ++E A+  A P  +  ++VE   +S  D+ G+DE  Q+++E +  P+  PE    MG+ +A  G+LLYGP GTGKT +  A A A +S+A+F++V+  E+ +K +GESE+A+   F+ AR  APC++F D +++IA   G +++   T +R+L+ LL EMDGL   + + D      V+V+AATN  ++LD A LRPGR D L+ +  PD++ R  IF +H    PL  +  +                  ET+AD +EG  GADI+ +C+EA ++ALRE+ D  Y+  RH  EAL
Sbjct:  233 KGVILYGPPGTGKTLIAKAVANESGASFHYIAGPEIVGKFYGESEERLRKIFEEATQDA------PSVIFIDEIDSIAPKRENVTGEVERRVVAQLLTLLDGMVERG-QVVVIGATNRVDAIDPALRRPGRFDREIHIGVPDTKDRYEILQIHTRGMPIERDEETEEIGKVETEADEAALERERKEKADKYLMYLAERTQGFVGADLLALVQEAAMRCLRENLPDLDLEKEAIPPERLEKIVVTKRNFEDALMEAEPSALREIYVEMPTVSWNDVGGLDEAKQSITEAVEWPIKNPEKFSHMGI-KAPRGILLYGPPGTGKTLI--AKAVAKESNANFISVKGPEIFSKWLGESEKAIRETFRKARQVAPCVIFFDEIDSIASMPGMESTDSHTSERVLNQLLTEMDGL---ESLRD------VVVIAATNRPNLLDPAILRPGRFDRLVYIGSPDRKGRLKIFRIHTKDTPLAENVNL------------------ETLADETEGYVGADIESVCREAVMIALRENFDTEYVEMRHFREAL 737          

HSP 2 Score: 88.1965 bits (217), Expect = 1.400e-14
Identity = 65/220 (29.55%), Postives = 111/220 (50.45%), Query Frame = 0

Query:   55 CQAVRGALLEGGPGAGKTALAKSLARHGGLPSYYCSCPSLFSRDRGDAERGLQEIFQTCIQKAACSEKESCLLVLDDLDCI----GKDRKKARHVSDLGILAMLLECLDHLRGAH-VFVLAIAGDASLIDVGLLQPGRLEITLKLSSLKQEQREKILANLLGKLNTKSPRAVEESSIQYSGRLLDSLACRTRGFNGADLAYLCSSALTSALRRSQSSDHV 269
             +A RG LL G PG GKT +AK++A+           P +FS+  G++E+ ++E F+   Q A       C++  D++D I    G +   + H S+  +L  LL  +D L     V V+A     +L+D  +L+PGR +  + + S  ++ R KI      +++TK     E  +       L++LA  T G+ GAD+  +C  A+  ALR +  +++V
Sbjct:  529 IKAPRGILLYGPPGTGKTLIAKAVAKESNANFISVKGPEIFSKWLGESEKAIRETFRKARQVA------PCVIFFDEIDSIASMPGMESTDS-HTSER-VLNQLLTEMDGLESLRDVVVIAATNRPNLLDPAILRPGRFDRLVYIGSPDRKGRLKIF-----RIHTKDTPLAENVN-------LETLADETEGYVGADIESVCREAVMIALRENFDTEYV 728          

BLAST of EWM27715.1 vs. NCBI_GenBank
Match: gi|156566470|gb|ABU81875.1| (AAA family ATPase, CDC48 subfamily [Ignicoccus hospitalis KIN4/I])

HSP 1 Score: 273.478 bits (698), Expect = 3.983e-79
Identity = 197/584 (33.73%), Postives = 305/584 (52.23%), Query Frame = 0

Query:   11 DKKCQHRR--SAIEADLPGLE-------EDIDLLLRLIDGFSRNGGGSRDQSFCQAVRGALLEGGPGAGKTALAKSLARHGGLPSYYCSCPSLFSRDRGDAERGLQEIFQTCIQKAACSEKESCLLVLDDLDCIGKDRKKARHVSDLGILAMLLECLDHL--RGAHVFVLAIAGDASLIDVGLLQPGRLEITLKLSSLKQEQREKILANLLGKLNTKSPRAVEESSIQYSGRLLDSLACRTRGFNGADLAYLCSSALTSALRRSQSS-------------DHVCIEEEDWEVAMEGAHPIDIPWLFVEGARL--SDLAGMDEIIQTVSETILQPLLQPEALRAMGVPRASFGVLLYGPSGTGKTTLGLALAAAAQSHASFLAVECSELVNKVVGESERAVARLFQVARHRAPCILFLDHVEAIAGRRGFDTSSEQTMDRMLSTLLMEMDGLTAGDCMHDRTGSDMVIVVAATNLLSMLDEAFLRPGRLDILIEMALPDKEARAAIFLLHFSRMPLRFDTAVRGLKSEKNSTFTPLHEFAETMADLSEGLSGADIQGLCQEAALLALREDLDRSYLLPRHVMEAL 568
            +K  +H R       D+ GLE       E I+L ++  + F R G         +  +G LL G PG GKT LAK+LA          + P + S+  G++E+ L+EIF+   + A        ++ +D++D I   R++     +  ++A LL  +D L  RG  V V+      + ID  L +PGRLE+ +++    ++ R +IL     +++T++    E+         L+ LA  T G+ GADLA L  SA   ALRR                 D + +  ED+  A +   P  +  +++E   +   D+ G+ E  Q + E +  PL  PEA R +G+     G+LL+GP GTGKT L  A AAA +S A+F+AV   E+++K VGESE+A+  +F+ AR  AP I+F D +++IA  RG DTS+ Q  +R++S LL EMDG+       +R G+  V+V+A+TN   M+D A LRPGR D LI +  PDKEAR  I  +H   MPL  D  +  L                  A+++EG +GAD++ LC+EA + A+RE+++ + +  RH + AL
Sbjct:  171 EKYVEHARVPKVTWEDIGGLENVVRKLRELIELPMKYPEIFKRLG--------IEPPKGVLLFGPPGTGKTMLAKALANEIDAHFIPINGPEIMSKYYGESEQRLREIFEEARKNA------PSIIFIDEIDAIAPKREEVTGEVEKRVVAQLLTLMDGLQERG-RVVVIGATNRPNAIDPALRRPGRLEVEIEIPLPDKKGRLEIL-----QIHTRNMPLAEDVD-------LEKLAEMTHGYTGADLAALVRSAAFYALRRYLPEIDLDKGEIPPDLLDRMKVTMEDFINAYKDIVPSGLREIYIETPEVHWEDVGGLKEAKQQLREAVEWPLKYPEAFRRLGI-EPPKGILLFGPPGTGKTLL--AKAAATESQANFIAVRGPEILSKWVGESEKAIREIFRKARQAAPTIVFFDEIDSIAPVRGMDTST-QVTERIVSQLLTEMDGI-------ERLGN--VVVIASTNRPDMVDPALLRPGRFDKLIYVPPPDKEARFQILKIHTRNMPLDMDVDLWRL------------------AEMTEGYTGADLEALCREAGMEAMRENINTTKVSMRHFLNAL 696          

BLAST of EWM27715.1 vs. NCBI_GenBank
Match: gi|1180238172|ref|WP_083756977.1| (AAA family ATPase [Ignicoccus hospitalis])

HSP 1 Score: 273.092 bits (697), Expect = 4.031e-79
Identity = 197/584 (33.73%), Postives = 305/584 (52.23%), Query Frame = 0

Query:   11 DKKCQHRR--SAIEADLPGLE-------EDIDLLLRLIDGFSRNGGGSRDQSFCQAVRGALLEGGPGAGKTALAKSLARHGGLPSYYCSCPSLFSRDRGDAERGLQEIFQTCIQKAACSEKESCLLVLDDLDCIGKDRKKARHVSDLGILAMLLECLDHL--RGAHVFVLAIAGDASLIDVGLLQPGRLEITLKLSSLKQEQREKILANLLGKLNTKSPRAVEESSIQYSGRLLDSLACRTRGFNGADLAYLCSSALTSALRRSQSS-------------DHVCIEEEDWEVAMEGAHPIDIPWLFVEGARL--SDLAGMDEIIQTVSETILQPLLQPEALRAMGVPRASFGVLLYGPSGTGKTTLGLALAAAAQSHASFLAVECSELVNKVVGESERAVARLFQVARHRAPCILFLDHVEAIAGRRGFDTSSEQTMDRMLSTLLMEMDGLTAGDCMHDRTGSDMVIVVAATNLLSMLDEAFLRPGRLDILIEMALPDKEARAAIFLLHFSRMPLRFDTAVRGLKSEKNSTFTPLHEFAETMADLSEGLSGADIQGLCQEAALLALREDLDRSYLLPRHVMEAL 568
            +K  +H R       D+ GLE       E I+L ++  + F R G         +  +G LL G PG GKT LAK+LA          + P + S+  G++E+ L+EIF+   + A        ++ +D++D I   R++     +  ++A LL  +D L  RG  V V+      + ID  L +PGRLE+ +++    ++ R +IL     +++T++    E+         L+ LA  T G+ GADLA L  SA   ALRR                 D + +  ED+  A +   P  +  +++E   +   D+ G+ E  Q + E +  PL  PEA R +G+     G+LL+GP GTGKT L  A AAA +S A+F+AV   E+++K VGESE+A+  +F+ AR  AP I+F D +++IA  RG DTS+ Q  +R++S LL EMDG+       +R G+  V+V+A+TN   M+D A LRPGR D LI +  PDKEAR  I  +H   MPL  D  +  L                  A+++EG +GAD++ LC+EA + A+RE+++ + +  RH + AL
Sbjct:  155 EKYVEHARVPKVTWEDIGGLENVVRKLRELIELPMKYPEIFKRLG--------IEPPKGVLLFGPPGTGKTMLAKALANEIDAHFIPINGPEIMSKYYGESEQRLREIFEEARKNA------PSIIFIDEIDAIAPKREEVTGEVEKRVVAQLLTLMDGLQERG-RVVVIGATNRPNAIDPALRRPGRLEVEIEIPLPDKKGRLEIL-----QIHTRNMPLAEDVD-------LEKLAEMTHGYTGADLAALVRSAAFYALRRYLPEIDLDKGEIPPDLLDRMKVTMEDFINAYKDIVPSGLREIYIETPEVHWEDVGGLKEAKQQLREAVEWPLKYPEAFRRLGI-EPPKGILLFGPPGTGKTLL--AKAAATESQANFIAVRGPEILSKWVGESEKAIREIFRKARQAAPTIVFFDEIDSIAPVRGMDTST-QVTERIVSQLLTEMDGI-------ERLGN--VVVIASTNRPDMVDPALLRPGRFDKLIYVPPPDKEARFQILKIHTRNMPLDMDVDLWRL------------------AEMTEGYTGADLEALCREAGMEAMRENINTTKVSMRHFLNAL 680          

BLAST of EWM27715.1 vs. NCBI_GenBank
Match: gi|1125708812|ref|WP_075050279.1| (AAA family ATPase [Ignicoccus islandicus])

HSP 1 Score: 272.322 bits (695), Expect = 7.915e-79
Identity = 183/536 (34.14%), Postives = 285/536 (53.17%), Query Frame = 0

Query:   55 CQAVRGALLEGGPGAGKTALAKSLARHGGLPSYYCSC--PSLFSRDRGDAERGLQEIFQTCIQKAACSEKESCLLVLDDLDCIGKDRKKARHVSDLGILAMLLECLDHL--RGAHVFVLAIAGDASLIDVGLLQPGRLEITLKLSSLKQEQREKILANLLGKLNTKSPRAVEESSIQYSGRLLDSLACRTRGFNGADLAYLCSSALTSALRR----------------SQSSDHVCIEEEDWEVAMEGAHPIDIPWLFVE--GARLSDLAGMDEIIQTVSETILQPLLQPEALRAMGVPRASFGVLLYGPSGTGKTTLGLALAAAAQSHASFLAVECSELVNKVVGESERAVARLFQVARHRAPCILFLDHVEAIAGRRGFDTSSEQTMDRMLSTLLMEMDGLTAGDCMHDRTGSDMVIVVAATNLLSMLDEAFLRPGRLDILIEMALPDKEARAAIFLLHFSRMPLRFDTAVRGLKSEKNSTFTPLHEFAETMADLSEGLSGADIQGLCQEAALLALREDLDRSYLLPRHVMEAL 568
             +  +G LL G PG GKT LAK+LA   G  +Y+ +   P + S+  G++E+ L+EIF+   + A        ++ +D++D I   R++     +  ++A LL  +D L  RG  V V+      + ID  L +PGR +  +++    +  R+ IL     +++T++    E+         LD +A  T G+ GADLA L   A  +ALRR                +   +++ +   D+  AM+   P  +  +++E    R  D+ G++E+ Q + E ++ PL  PE    MG+     G+LL+GP GTGKT L  A AAA +S A+F+AV   E+++K VGESE+A+  +F+ AR  AP I+F D +++IA RRG D S    +DR+++ +L EMDG+              V V+AATN   +LD A LRPGR D LI +  PDK+AR  IF +H  RMPL  D  +                  E +A+++EG +GADI+ +C+EAAL ALRE++    +  RH  +AL
Sbjct:  213 IEPPKGVLLYGPPGTGKTMLAKALANEIG--AYFIAINGPEIMSKYYGESEQRLREIFEEARKNA------PSIIFIDEIDAIAPKREEVTGEVEKRVVAQLLTLMDGLQERG-RVVVIGATNRPNAIDPALRRPGRFDREIEIPPPDKRARKAIL-----EVHTRNVPLAEDVD-------LDKIAEMTHGYTGADLAALVKEAAMNALRRFFKEKGIDLTKVEKIPTTELENLKVTFRDFLNAMKVIQPTLMREVYIEVPEVRWEDIGGLEEVKQQLKEAVVWPLKHPEFFTEMGI-EPPKGILLFGPPGTGKTML--AKAAATESQANFIAVRGPEILSKWVGESEKAIREIFRKARQAAPTIIFFDEIDSIAVRRGRDVSG--VLDRIVNQILTEMDGIEP---------LQRVTVIAATNRPDLLDPALLRPGRFDRLIYVPPPDKKARLEIFKVHTRRMPLADDVDL------------------EKLAEITEGYTGADIEAVCREAALTALRENMKPVPVTMRHFEKAL 695          

HSP 2 Score: 144.436 bits (363), Expect = 6.936e-33
Identity = 107/340 (31.47%), Postives = 166/340 (48.82%), Query Frame = 0

Query:  243 FNGADLAYLCSSALTSALRRSQSSDHVCIEEEDW-EVAMEGAHPIDIPWLFVEGARLSDLAGMDEIIQTVSETILQPLLQPEALRAMGVPRASFGVLLYGPSGTGKTTLGLALAAAAQSHASFLAVECSELVNKVVGESERAVARLFQVARHRAPCILFLDHVEAIAGRRGFDTSSEQTMDRMLSTLLMEMDGLTAGDCMHDRTGSDMVIVVAATNLLSMLDEAFLRPGRLDILIEMALPDKEARAAIFLLHFSRMPLRFDTAVRGLKSEKNSTFTPLHEFAETMADLSEGLSGADIQGLCQEAALLALRE-------DLDRSYLLPRHVMEALDVKRRN 574
            F G+ L  L  S   S       +  + I ++   E A++G     + W         D+  ++E  + + E +  P+  PE  R +G+     GVLLYGP GTGKT L  AL  A +  A F+A+   E+++K  GESE+ +  +F+ AR  AP I+F+D ++AIA +R  +  + +   R+++ LL  MDGL              V+V+ ATN  + +D A  RPGR D  IE+  PDK AR AI  +H   +PL  D  +                  + +A+++ G +GAD+  L +EAA+ ALR        DL +   +P   +E L V  R+
Sbjct:  135 FFGSTLELLVVSTQPSTRVYVTENTEISISKKPVKEEALKGVP--KVTW--------EDIGDLEEAKERLREIVELPMKHPEVFRHLGI-EPPKGVLLYGPPGTGKTMLAKAL--ANEIGAYFIAINGPEIMSKYYGESEQRLREIFEEARKNAPSIIFIDEIDAIAPKR--EEVTGEVEKRVVAQLLTLMDGLQE---------RGRVVVIGATNRPNAIDPALRRPGRFDREIEIPPPDKRARKAILEVHTRNVPLAEDVDL------------------DKIAEMTHGYTGADLAALVKEAAMNALRRFFKEKGIDLTKVEKIPTTELENLKVTFRD 432          

BLAST of EWM27715.1 vs. NCBI_GenBank
Match: gi|969856108|gb|ALU11974.1| (ATPase AAA [Ignicoccus islandicus DSM 13165])

HSP 1 Score: 271.552 bits (693), Expect = 2.811e-78
Identity = 183/536 (34.14%), Postives = 285/536 (53.17%), Query Frame = 0

Query:   55 CQAVRGALLEGGPGAGKTALAKSLARHGGLPSYYCSC--PSLFSRDRGDAERGLQEIFQTCIQKAACSEKESCLLVLDDLDCIGKDRKKARHVSDLGILAMLLECLDHL--RGAHVFVLAIAGDASLIDVGLLQPGRLEITLKLSSLKQEQREKILANLLGKLNTKSPRAVEESSIQYSGRLLDSLACRTRGFNGADLAYLCSSALTSALRR----------------SQSSDHVCIEEEDWEVAMEGAHPIDIPWLFVE--GARLSDLAGMDEIIQTVSETILQPLLQPEALRAMGVPRASFGVLLYGPSGTGKTTLGLALAAAAQSHASFLAVECSELVNKVVGESERAVARLFQVARHRAPCILFLDHVEAIAGRRGFDTSSEQTMDRMLSTLLMEMDGLTAGDCMHDRTGSDMVIVVAATNLLSMLDEAFLRPGRLDILIEMALPDKEARAAIFLLHFSRMPLRFDTAVRGLKSEKNSTFTPLHEFAETMADLSEGLSGADIQGLCQEAALLALREDLDRSYLLPRHVMEAL 568
             +  +G LL G PG GKT LAK+LA   G  +Y+ +   P + S+  G++E+ L+EIF+   + A        ++ +D++D I   R++     +  ++A LL  +D L  RG  V V+      + ID  L +PGR +  +++    +  R+ IL     +++T++    E+         LD +A  T G+ GADLA L   A  +ALRR                +   +++ +   D+  AM+   P  +  +++E    R  D+ G++E+ Q + E ++ PL  PE    MG+     G+LL+GP GTGKT L  A AAA +S A+F+AV   E+++K VGESE+A+  +F+ AR  AP I+F D +++IA RRG D S    +DR+++ +L EMDG+              V V+AATN   +LD A LRPGR D LI +  PDK+AR  IF +H  RMPL  D  +                  E +A+++EG +GADI+ +C+EAAL ALRE++    +  RH  +AL
Sbjct:  248 IEPPKGVLLYGPPGTGKTMLAKALANEIG--AYFIAINGPEIMSKYYGESEQRLREIFEEARKNA------PSIIFIDEIDAIAPKREEVTGEVEKRVVAQLLTLMDGLQERG-RVVVIGATNRPNAIDPALRRPGRFDREIEIPPPDKRARKAIL-----EVHTRNVPLAEDVD-------LDKIAEMTHGYTGADLAALVKEAAMNALRRFFKEKGIDLTKVEKIPTTELENLKVTFRDFLNAMKVIQPTLMREVYIEVPEVRWEDIGGLEEVKQQLKEAVVWPLKHPEFFTEMGI-EPPKGILLFGPPGTGKTML--AKAAATESQANFIAVRGPEILSKWVGESEKAIREIFRKARQAAPTIIFFDEIDSIAVRRGRDVSG--VLDRIVNQILTEMDGIEP---------LQRVTVIAATNRPDLLDPALLRPGRFDRLIYVPPPDKKARLEIFKVHTRRMPLADDVDL------------------EKLAEITEGYTGADIEAVCREAALTALRENMKPVPVTMRHFEKAL 730          

HSP 2 Score: 144.436 bits (363), Expect = 9.555e-33
Identity = 107/340 (31.47%), Postives = 166/340 (48.82%), Query Frame = 0

Query:  243 FNGADLAYLCSSALTSALRRSQSSDHVCIEEEDW-EVAMEGAHPIDIPWLFVEGARLSDLAGMDEIIQTVSETILQPLLQPEALRAMGVPRASFGVLLYGPSGTGKTTLGLALAAAAQSHASFLAVECSELVNKVVGESERAVARLFQVARHRAPCILFLDHVEAIAGRRGFDTSSEQTMDRMLSTLLMEMDGLTAGDCMHDRTGSDMVIVVAATNLLSMLDEAFLRPGRLDILIEMALPDKEARAAIFLLHFSRMPLRFDTAVRGLKSEKNSTFTPLHEFAETMADLSEGLSGADIQGLCQEAALLALRE-------DLDRSYLLPRHVMEALDVKRRN 574
            F G+ L  L  S   S       +  + I ++   E A++G     + W         D+  ++E  + + E +  P+  PE  R +G+     GVLLYGP GTGKT L  AL  A +  A F+A+   E+++K  GESE+ +  +F+ AR  AP I+F+D ++AIA +R  +  + +   R+++ LL  MDGL              V+V+ ATN  + +D A  RPGR D  IE+  PDK AR AI  +H   +PL  D  +                  + +A+++ G +GAD+  L +EAA+ ALR        DL +   +P   +E L V  R+
Sbjct:  170 FFGSTLELLVVSTQPSTRVYVTENTEISISKKPVKEEALKGVP--KVTW--------EDIGDLEEAKERLREIVELPMKHPEVFRHLGI-EPPKGVLLYGPPGTGKTMLAKAL--ANEIGAYFIAINGPEIMSKYYGESEQRLREIFEEARKNAPSIIFIDEIDAIAPKR--EEVTGEVEKRVVAQLLTLMDGLQE---------RGRVVVIGATNRPNAIDPALRRPGRFDREIEIPPPDKRARKAILEVHTRNVPLAEDVDL------------------DKIAEMTHGYTGADLAALVKEAAMNALRRFFKEKGIDLTKVEKIPTTELENLKVTFRD 467          

BLAST of EWM27715.1 vs. NCBI_GenBank
Match: gi|503564455|ref|WP_013798531.1| (ATPase AAA [Methanotorris igneus] >gi|333750843|gb|AEF95922.1| AAA family ATPase, CDC48 subfamily [Methanotorris igneus Kol 5])

HSP 1 Score: 270.781 bits (691), Expect = 3.738e-78
Identity = 190/584 (32.53%), Postives = 299/584 (51.20%), Query Frame = 0

Query:    8 PHSDKKCQHRRSAIEADLPGLEEDI-------DLLLRLIDGFSRNGGGSRDQSFCQAVRGALLEGGPGAGKTALAKSLARHGGLPSYYCSCPSLFSRDRGDAERGLQEIFQTCIQKAACSEKESCLLVLDDLDCIGKDRKKARHVSDLGILAMLLECLDHLRG-AHVFVLAIAGDASLIDVGLLQPGRLEITLKLSSLKQEQREKILANLLGKLNTKSPRAVEESSIQYSGRLLDSLACRTRGFNGADLAYLCSSALTSALRR-------------SQSSDHVCIEEEDWEVAMEGAHPIDIPWLFVE--GARLSDLAGMDEIIQTVSETILQPLLQPEALRAMGVPRASFGVLLYGPSGTGKTTLGLALAAAAQSHASFLAVECSELVNKVVGESERAVARLFQVARHRAPCILFLDHVEAIAGRRGFDTSSEQTMDRMLSTLLMEMDGLTAGDCMHDRTGSDMVIVVAATNLLSMLDEAFLRPGRLDILIEMALPDKEARAAIFLLHFSRMPLRFDTAVRGLKSEKNSTFTPLHEFAETMADLSEGLSGADIQGLCQEAALLALREDLDRSYLLPRHVMEAL 568
            P  + K     S    D+ GL+E++       +L +R  + F R G         +  +G LL G PG GKT LAK++A   G   Y  + P + S+  G+ E  L++IFQ         E    ++ +D++D I   R +A    +  ++A LL  +D L     V V+A       +D  L +PGR +  + +    +  R++IL     +++T++    ++  + Y       LA  T GF GADLA LC  A    LRR              +  D + +  +D++ A++   P  +  + VE    +  D+ G++++ Q + E +  PL   +    MG+ R   GVLL+GP GTGKT L  A A A +S A+F++V+  E+ +K VGESE+A+  +F+ AR  APCI+F D +++IA RRG    S  T +++++ LL E+DGL              V+V+AATN   +LD A LRPGRLD ++ +  PDK+AR AIF +H  +MPL  D  +                  E +A+ +EG +GADI+ +C+EAA+LALRE+++   +  RH  EAL
Sbjct:  164 PVKEIKESKVPSVTYEDIGGLKEEVRKIREMVELPMRHPELFERLG--------IEPPKGVLLAGPPGTGKTLLAKAVANEAGANFYSINGPEILSKYVGETEENLRKIFQEA------EENAPSVIFIDEIDAIAPKRDEATGEVERRMVAQLLTLMDGLESRGQVVVIAATNRPDALDPALRRPGRFDREIVIGVPDRNARKEIL-----QIHTRNMPLAKDVDLDY-------LADVTHGFVGADLAALCKEAAMKTLRRILPDLDLDKDEIPKEVLDSIEVTMDDFKEALKEVEPSALREVLVEVPNVKWDDIGGLEDVKQELREAVEWPLKHRDVFERMGI-RPPRGVLLFGPPGTGKTLL--AKAVANESEANFISVKGPEIFSKWVGESEKAIREIFRKARQTAPCIIFFDEIDSIAPRRGSGHDSGVT-EKVVNQLLTELDGLEE---------PKDVVVIAATNRPDILDPALLRPGRLDRIVLVPAPDKKARLAIFKVHTRKMPLADDVDL------------------EKLAEKTEGYTGADIEAVCREAAMLALRENINAEKVEMRHFEEAL 690          

HSP 2 Score: 154.836 bits (390), Expect = 2.933e-36
Identity = 96/283 (33.92%), Postives = 151/283 (53.36%), Query Frame = 0

Query:  293 VEGARLSDLAGMDEIIQTVSETILQPLLQPEALRAMGVPRASFGVLLYGPSGTGKTTLGLALAAAAQSHASFLAVECSELVNKVVGESERAVARLFQVARHRAPCILFLDHVEAIAGRRGFDTSSEQTMDRMLSTLLMEMDGLTAGDCMHDRTGSDMVIVVAATNLLSMLDEAFLRPGRLDILIEMALPDKEARAAIFLLHFSRMPLRFDTAVRGLKSEKNSTFTPLHEFAETMADLSEGLSGADIQGLCQEAALLALRE-----DLDRSYLLPRHVMEALDV 570
            V      D+ G+ E ++ + E +  P+  PE    +G+     GVLL GP GTGKT   LA A A ++ A+F ++   E+++K VGE+E  + ++FQ A   AP ++F+D ++AIA +R  D ++ +   RM++ LL  MDGL +            V+V+AATN    LD A  RPGR D  I + +PD+ AR  I  +H   MPL  D  +                  + +AD++ G  GAD+  LC+EAA+  LR      DLD+   +P+ V+++++V
Sbjct:  173 VPSVTYEDIGGLKEEVRKIREMVELPMRHPELFERLGI-EPPKGVLLAGPPGTGKTL--LAKAVANEAGANFYSINGPEILSKYVGETEENLRKIFQEAEENAPSVIFIDEIDAIAPKR--DEATGEVERRMVAQLLTLMDGLES---------RGQVVVIAATNRPDALDPALRRPGRFDREIVIGVPDRNARKEILQIHTRNMPLAKDVDL------------------DYLADVTHGFVGADLAALCKEAAMKTLRRILPDLDLDKDE-IPKEVLDSIEV 422          

BLAST of EWM27715.1 vs. NCBI_GenBank
Match: gi|494103500|ref|WP_007044287.1| (ATPase AAA [Methanotorris formicicus] >gi|373560840|gb|EHP87090.1| AAA family ATPase, CDC48 subfamily [Methanotorris formicicus Mc-S-70])

HSP 1 Score: 270.011 bits (689), Expect = 6.553e-78
Identity = 190/584 (32.53%), Postives = 296/584 (50.68%), Query Frame = 0

Query:    8 PHSDKKCQHRRSAIEADLPGLEEDI-------DLLLRLIDGFSRNGGGSRDQSFCQAVRGALLEGGPGAGKTALAKSLARHGGLPSYYCSCPSLFSRDRGDAERGLQEIFQTCIQKAACSEKESCLLVLDDLDCIGKDRKKARHVSDLGILAMLLECLDHLRG-AHVFVLAIAGDASLIDVGLLQPGRLEITLKLSSLKQEQREKILANLLGKLNTKSPRAVEESSIQYSGRLLDSLACRTRGFNGADLAYLCSSALTSALRR-------------SQSSDHVCIEEEDWEVAMEGAHPIDIPWLFVE--GARLSDLAGMDEIIQTVSETILQPLLQPEALRAMGVPRASFGVLLYGPSGTGKTTLGLALAAAAQSHASFLAVECSELVNKVVGESERAVARLFQVARHRAPCILFLDHVEAIAGRRGFDTSSEQTMDRMLSTLLMEMDGLTAGDCMHDRTGSDMVIVVAATNLLSMLDEAFLRPGRLDILIEMALPDKEARAAIFLLHFSRMPLRFDTAVRGLKSEKNSTFTPLHEFAETMADLSEGLSGADIQGLCQEAALLALREDLDRSYLLPRHVMEAL 568
            P  + K     S    D+ GL+E++       +L +R  + F R G         +  +G LL G PG GKT LAK++A   G   Y  + P + S+  G+ E  L++IFQ         E    ++ +D++D I   R +A    +  ++A LL  +D L G   V V+A       +D  L +PGR +  + +    +  R++IL     +++T++    E+ ++ Y       LA  T GF GADLA LC  A    LRR                 D + +  +D++ A++   P  +  + VE    +  D+ G++E+ Q + E +  PL   E    MG+ R   GVLL+GP GTGKT L  A A A +S A+F++V+  E+ +K VGESE+A+  +F+ AR  AP ++F D +++IA RRG    S  T +++++ LL E+DGL              V+V+AATN   +LD A LRPGRLD ++ +  PDK+ R +IF +H   MPL  D  +                  E +A+ +EG +GADI+ +C+EAA+LALRE++    +  RH  EAL
Sbjct:  164 PVKEIKESKVPSVTYEDIGGLKEEVRKIREMVELPMRHPELFERLG--------IEPPKGVLLAGPPGTGKTLLAKAVANEAGANFYSINGPEIMSKYVGETEENLRKIFQEA------EENAPSVIFIDEIDAIAPKRDEATGEVERRMVAQLLTLMDGLEGRGQVVVIAATNRPDALDSALRRPGRFDREIVIGVPDRNARKEIL-----QIHTRNMPLAEDVNLDY-------LADVTHGFVGADLAALCKEAAMKTLRRILPDLDLDKDEIPKDILDSIEVTMDDFKEALKEVEPSALREVLVEVPNVKWDDIGGLEEVKQELKEAVEWPLKHKEVFERMGI-RPPRGVLLFGPPGTGKTLL--AKAVANESEANFISVKGPEIFSKWVGESEKAIREIFRKARQTAPTVIFFDEIDSIAPRRGSGHDSGVT-EKVVNQLLTELDGLEE---------PKDVVVIAATNRPDILDPALLRPGRLDRIVFVPAPDKKTRLSIFKVHTKNMPLAEDVDL------------------EKLAEKTEGYTGADIEAICREAAMLALRENMKADKVEMRHFEEAL 690          

HSP 2 Score: 155.606 bits (392), Expect = 1.369e-36
Identity = 96/283 (33.92%), Postives = 151/283 (53.36%), Query Frame = 0

Query:  293 VEGARLSDLAGMDEIIQTVSETILQPLLQPEALRAMGVPRASFGVLLYGPSGTGKTTLGLALAAAAQSHASFLAVECSELVNKVVGESERAVARLFQVARHRAPCILFLDHVEAIAGRRGFDTSSEQTMDRMLSTLLMEMDGLTAGDCMHDRTGSDMVIVVAATNLLSMLDEAFLRPGRLDILIEMALPDKEARAAIFLLHFSRMPLRFDTAVRGLKSEKNSTFTPLHEFAETMADLSEGLSGADIQGLCQEAALLALRE-----DLDRSYLLPRHVMEALDV 570
            V      D+ G+ E ++ + E +  P+  PE    +G+     GVLL GP GTGKT   LA A A ++ A+F ++   E+++K VGE+E  + ++FQ A   AP ++F+D ++AIA +R  D ++ +   RM++ LL  MDGL          G   V+V+AATN    LD A  RPGR D  I + +PD+ AR  I  +H   MPL  D  +                  + +AD++ G  GAD+  LC+EAA+  LR      DLD+   +P+ ++++++V
Sbjct:  173 VPSVTYEDIGGLKEEVRKIREMVELPMRHPELFERLGI-EPPKGVLLAGPPGTGKTL--LAKAVANEAGANFYSINGPEIMSKYVGETEENLRKIFQEAEENAPSVIFIDEIDAIAPKR--DEATGEVERRMVAQLLTLMDGL---------EGRGQVVVIAATNRPDALDSALRRPGRFDREIVIGVPDRNARKEILQIHTRNMPLAEDVNL------------------DYLADVTHGFVGADLAALCKEAAMKTLRRILPDLDLDKDE-IPKDILDSIEV 422          

BLAST of EWM27715.1 vs. NCBI_GenBank
Match: gi|850927564|ref|WP_048057848.1| (ATPase AAA [Methanothermococcus okinawensis])

HSP 1 Score: 270.011 bits (689), Expect = 8.342e-78
Identity = 179/557 (32.14%), Postives = 288/557 (51.71%), Query Frame = 0

Query:   28 LEEDIDLLLRLIDGFSRNGGGSRDQSFCQAVRGALLEGGPGAGKTALAKSLARHGGLPSYYCSCPSLFSRDRGDAERGLQEIFQTCIQKAACSEKESCLLVLDDLDCIGKDRKKARHVSDLGILAMLLECLDHLRG-AHVFVLAIAGDASLIDVGLLQPGRLEITLKLSSLKQEQREKILANLLGKLNTKSPRAVEESSIQYSGRLLDSLACRTRGFNGADLAYLCSSALTSALRR-------------SQSSDHVCIEEEDWEVAMEGAHPIDIPWLFVE--GARLSDLAGMDEIIQTVSETILQPLLQPEALRAMGVPRASFGVLLYGPSGTGKTTLGLALAAAAQSHASFLAVECSELVNKVVGESERAVARLFQVARHRAPCILFLDHVEAIAGRRGFDTSSEQTMDRMLSTLLMEMDGLTAGDCMHDRTGSDMVIVVAATNLLSMLDEAFLRPGRLDILIEMALPDKEARAAIFLLHFSRMPLRFDTAVRGLKSEKNSTFTPLHEFAETMADLSEGLSGADIQGLCQEAALLALREDLDRSYLLPRHVMEAL 568
            + E ++L +R  + F + G         +  +G LL G PG GKT LAK++A   G   Y  + P + S+  G+ E  L++IF+         E+   ++ +D++D I   R +A    +  ++A LL  +D L     V V+A       +D  L +PGR +  + +    ++ R++IL     +++T++    E+         LD LA  T GF GADLA LC  A    LRR              +  D + +  +D++ A++   P  +  + VE    +  D+ G++E+ Q + E +  PL   E    MG+ R   GVLL+GP GTGKT L  A A A +S A+F++V+  E+ +K VGESE+A+  +F+ AR  AP ++F D +++IA RRG D       +++++ LL E+DGL              V+++AATN   +LD A LRPGRLD ++ + +PDK+AR  I  +H  +MPL  D  ++ L                  A+ +EG +GAD++ +C+EAA++ALRE+L    +  RH  EAL
Sbjct:  189 IREMVELPMRYPELFDKLG--------IEPPKGVLLAGPPGTGKTLLAKAVANEAGANFYTINGPEIMSKYVGETEENLRKIFEEA------EEESPSIVFIDEIDAIAPKRDEASGEVERRMVAQLLTLMDGLESRGQVVVIAATNRPDALDPALRRPGRFDREITIGVPDRKGRKEIL-----QIHTRNMPLAEDVD-------LDYLADVTHGFVGADLAALCKEAAMKTLRRLLPDIDLEKEEIPKEILDKIEVTMQDFKEALKEVEPSALREVLVEVPNVKWDDIGGLEEVKQDLKEAVEWPLKYKEVFEKMGI-RPPKGVLLFGPPGTGKTLL--AKAVANESQANFISVKGPEIFSKWVGESEKAIREIFRKARQAAPTVVFFDEIDSIAPRRGSDIGGSGVAEKVVNQLLTELDGLEE---------PKDVVIIAATNRPDILDPALLRPGRLDRIVLVPVPDKKARYEILKVHTKKMPLAEDVDLKKL------------------AEKTEGYTGADLEAVCREAAMIALRENLKAEKVELRHFEEAL 689