EWM28782.1, cds1965 (CDS) Nannochloropsis gaditana

Overview
NameEWM28782.1
Unique Namecds1965
TypeCDS
OrganismNannochloropsis gaditana (N. gaditana B-31)
Alignment locationCM002458.1:595671..596230 +
Alignment locationCM002458.1:596351..596789 +

Link to JBrowse

Properties
Property NameValue
Protein idEWM28782.1
Productrna (guanine-9-)-methyltransferase domain-containing
Orig transcript idgnl|cribi|Naga_100002g48.4901.mrna
GbkeyCDS
Mutants
Expression
No biomaterial libraries express this feature.
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
CM002458.1supercontigCM002458.1:595671..596230 +
CM002458.1supercontigCM002458.1:596351..596789 +
Analyses
This CDS is derived from or has results from the following analyses
Analysis NameDate Performed
GO annotation for N. gaditana B312020-04-08
BLAST analysis for N. gaditana B-312020-04-07
InterPro analysis for N. gaditana B-312020-04-06
Gene prediction for N. gaditana B-312014-02-18
Annotated Terms
The following terms have been associated with this CDS:
Vocabulary: Molecular Function
TermDefinition
GO:0016740transferase activity
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR028564MT_TRM10-typ
IPR007356tRNA_m1G_MeTrfase_euk
IPR016009tRNA_MeTrfase_TRMD/TRM10
Homology
BLAST of EWM28782.1 vs. NCBI_GenBank
Match: gi|585111205|gb|EWM28782.1| (rna (guanine-9-)-methyltransferase domain-containing [Nannochloropsis gaditana])

HSP 1 Score: 691.034 bits (1782), Expect = 0.000e+0
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 0
Query:    1 MEPKRSANSAAEAVRDASEDTLSIPAPPSASAPSSSTPVKGGNLFLFRKPSRVKDVSKSVAKKLWRQEMWKVKKATKKAAKRAARQETNRQSGEVGQDDNLNLQAATSESKNDQLLRKQRREQRKNQRHEAQMAFLRRCEEGARVVIDCGFDSHMTEKELKSLGQQLMYVYASNKRALAPCTVHIAELGAGIQQRLSCVAGVQNWQGFNRETRPFMETYAAERDKLVYLTSDSPNVLTQLDPGLIYVIGGIVDRNRLKHATYNKALAQGIATARFPLREHVQLGSSPVLAVNHVFDILLEFQYTNDWRATFAKVLPNRKSVKVNEPAVNKDS 332
            MEPKRSANSAAEAVRDASEDTLSIPAPPSASAPSSSTPVKGGNLFLFRKPSRVKDVSKSVAKKLWRQEMWKVKKATKKAAKRAARQETNRQSGEVGQDDNLNLQAATSESKNDQLLRKQRREQRKNQRHEAQMAFLRRCEEGARVVIDCGFDSHMTEKELKSLGQQLMYVYASNKRALAPCTVHIAELGAGIQQRLSCVAGVQNWQGFNRETRPFMETYAAERDKLVYLTSDSPNVLTQLDPGLIYVIGGIVDRNRLKHATYNKALAQGIATARFPLREHVQLGSSPVLAVNHVFDILLEFQYTNDWRATFAKVLPNRKSVKVNEPAVNKDS
Sbjct:    1 MEPKRSANSAAEAVRDASEDTLSIPAPPSASAPSSSTPVKGGNLFLFRKPSRVKDVSKSVAKKLWRQEMWKVKKATKKAAKRAARQETNRQSGEVGQDDNLNLQAATSESKNDQLLRKQRREQRKNQRHEAQMAFLRRCEEGARVVIDCGFDSHMTEKELKSLGQQLMYVYASNKRALAPCTVHIAELGAGIQQRLSCVAGVQNWQGFNRETRPFMETYAAERDKLVYLTSDSPNVLTQLDPGLIYVIGGIVDRNRLKHATYNKALAQGIATARFPLREHVQLGSSPVLAVNHVFDILLEFQYTNDWRATFAKVLPNRKSVKVNEPAVNKDS 332          
BLAST of EWM28782.1 vs. NCBI_GenBank
Match: gi|1214710777|gb|OWZ21837.1| (RNA (guanine-9-)-methyltransferase [Phytophthora megakarya])

HSP 1 Score: 192.2 bits (487), Expect = 4.449e-56
Identity = 103/271 (38.01%), Postives = 157/271 (57.93%), Query Frame = 0
Query:   56 VSKSVAKKLWRQEMWKVKKATKKAAKRAARQETNRQSGEVGQDDNLNLQAATSESKNDQLLRKQRREQRKNQRHEAQMAFLRRCEEGARVVIDCGFDSHMTEKELKSLGQQLMYVYASNKRALAPCTVHIAELGAGIQQRLSCVAGVQNWQGFNRETRPFMETYAAERDKLVYLTSDSPNVLTQLDPGLIYVIGGIVDRNRLKHATYNKALAQGIATARFPLREHVQLGSSP-VLAVNHVFDILLEFQYTNDWRATFAKVLPNRKSVKVNE 325
            +SK   +K  R+  W+ +K  KK  KR   Q   + +G +     L++         + +LR++ R   K +      A+L   EEG +VVIDCGF+ +MTEKE +SL QQ+M+ Y  N+R+  P   +I  L   I++ L  ++G   WQ F   +  +++ +  +++ LVYLT+DSPN +++L    +Y+IGGIVDRNRLK  TY KA+ +GI TA+ PL   V+LGS+  VL VNHVF+IL +F    DW       LP+RK  ++ +
Sbjct:   10 LSKRALRKEKRRMQWEERKVEKKL-KRQENQRVKKANGLIPPPKELDMS-------EEAVLRRKERAMAKRE------AYLMAAEEGVKVVIDCGFEENMTEKEKRSLSQQIMFSYGVNRRSPTPMNTYITSLHGDIKKNLEKISGFHEWQAFTGSSNSYLDLF--KKESLVYLTADSPNTISKLSRDKVYIIGGIVDRNRLKGITYQKAVEEGIETAKLPLDAVVELGSATRVLTVNHVFEILAQFSEIKDWAQATLATLPSRKGAQLKQ 264          
BLAST of EWM28782.1 vs. NCBI_GenBank
Match: gi|1173949772|gb|OQR97776.1| (RNA (guanine-9-)-methyltransferase domain-containing protein [Achlya hypogyna])

HSP 1 Score: 192.2 bits (487), Expect = 7.930e-56
Identity = 105/271 (38.75%), Postives = 152/271 (56.09%), Query Frame = 0
Query:   54 KDVSKSVAKKLWRQEMWKVKKATKKAAKRAARQETNRQSGEVGQDDNLNLQAATSESKNDQLLRKQRREQRKNQRHEAQMAFLRRCEEGARVVIDCGFDSHMTEKELKSLGQQLMYVYASNKRALAPCTVHIAELGAGIQQRLSCVAGVQNWQGFNRETRPFMETYAAERDKLVYLTSDSPNVLTQLDPGLIYVIGGIVDRNRLKHATYNKALAQGIATARFPLREHVQLG-SSPVLAVNHVFDILLEFQYTNDWRATFAKVLPNRKSVKV 323
            K+  K   ++  RQE+W   K  K+A K  AR    +    V     L++  A   ++ ++ + K+              +FL    EG  +VIDCGF+  M+ KE KSL QQ+M+ Y  NKR  +P TV +  L    +  L  + G   W  F+  T+ +M+T+   ++ LVYLT+DSPNV+ +L    +Y+IGGIVDRNRLK ATY KA+AQGIATA+ PL + V++G ++ VL VNHVF IL+++Q   DW       LP RK   +
Sbjct:   28 KNSGKRALRRAKRQEIWVSVKERKRAKKEEARAARPKAPEPV-----LDMSEAAVLARRERTIAKRE-------------SFLMAANEGPTIVIDCGFEEDMSSKEKKSLSQQIMFSYGINKRCESPATVFLTSLHGETEANLQKIGGFSEWLAFDATTKCYMDTF--RKESLVYLTADSPNVIEELSSDKVYIIGGIVDRNRLKGATYEKAVAQGIATAKLPLDKVVEMGQATRVLTVNHVFQILVDYQTKQDWTQATMSALPARKGAHI 278          
BLAST of EWM28782.1 vs. NCBI_GenBank
Match: gi|953499128|emb|CEG39440.1| (rna (guanine-9-)-methyltransferase domain-containing [Plasmopara halstedii])

HSP 1 Score: 187.193 bits (474), Expect = 7.823e-54
Identity = 96/211 (45.50%), Postives = 133/211 (63.03%), Query Frame = 0
Query:  119 QRREQRKNQRHEAQMAFLRRCEEGARVVIDCGFDSHMTEKELKSLGQQLMYVYASNKRALAPCTVHIAELGAGIQQRLSCVAGVQNWQGFNRETRPFMETYAAERDKLVYLTSDSPNVLTQLDPGLIYVIGGIVDRNRLKHATYNKALAQGIATARFPLREHVQLGSSP-VLAVNHVFDILLEFQYTNDW-RATFAKVLPNRKSVKVNEPA 327
            +RRE+   +R    MA     EEG++VVIDC FD  M+EKE KSL QQ+M+ Y  N+R+  P  V+I  L   +++ L  ++G   WQ F   +  +M+ +  +++ LVYLT+DSPN +T+L    +Y+IGGIVDRNRLK  TY KA+ QGI TA+ PL   V +GS+  VL VNHVF+IL  F    +W +AT A  LP+RK V   E +
Sbjct:   84 RRRERNIAKRETLLMA----AEEGSKVVIDCAFDDKMSEKEKKSLSQQIMFSYGVNRRSSMPMNVYITSLHGDVRKNLEKISGFHEWQTFAESSLSYMDIF--KKESLVYLTADSPNTITKLCRDKVYIIGGIVDRNRLKGITYEKAIKQGIETAKLPLDAVVDMGSATRVLTVNHVFEILTHFSKVKNWAQATLA-TLPSRKGVHFKETS 287          
BLAST of EWM28782.1 vs. NCBI_GenBank
Match: gi|695376083|ref|XP_009515698.1| (hypothetical protein PHYSODRAFT_477148 [Phytophthora sojae] >gi|348688609|gb|EGZ28423.1| hypothetical protein PHYSODRAFT_477148 [Phytophthora sojae])

HSP 1 Score: 186.037 bits (471), Expect = 3.006e-53
Identity = 109/271 (40.22%), Postives = 159/271 (58.67%), Query Frame = 0
Query:   56 VSKSVAKKLWRQEMWKVKKATKKAAKRAARQETNRQSGEVGQDDNLNLQAATSESKNDQLLRKQRREQRKNQRHEAQMAFLRRCEEGARVVIDCGFDSHMTEKELKSLGQQLMYVYASNKRALAPCTVHIAELGAGIQQRLSCVAGVQNWQGFNRETRPFMETYAAERDKLVYLTSDSPNVLTQLDPGLIYVIGGIVDRNRLKHATYNKALAQGIATARFPLREHVQLGSSP-VLAVNHVFDILLEFQYTNDWRATFAKVLPNRKSVKVNE 325
            +SK   +K   +  W+ +KA+KK  KR  +Q   + SGE+     L++         + +LR++ R   K +      AFL   EEG +VVIDC F+  MTEKE KSL QQ+M+ Y  N+R+  P + +I  L   IQ+ L  ++G   WQ F    + +++ +  +++ LVYLT+DSPN +T+L    +Y+IGGIVDRNRLK  TY KA+ QGI TA+ PL   V++GS+  VL VNHVF+IL +F    DW       LP+RK  ++ E
Sbjct:   42 LSKRAKRKQKHRMQWEERKASKKL-KRQEQQRLKKASGEIPPPKELDMS-------EEAVLRRKERALAKRE------AFLMAAEEGVKVVIDCEFEEEMTEKEKKSLSQQIMFSYGVNRRSRTPMSAYITSLHGDIQKNLEKISGFHEWQAFTGSQKSYIDLF--KKESLVYLTADSPNTITKLSRDKVYIIGGIVDRNRLKGITYQKAVEQGIETAKLPLDTVVEMGSATRVLTVNHVFEILAQFSEVKDWAQATLSTLPSRKGAQLKE 296          
BLAST of EWM28782.1 vs. NCBI_GenBank
Match: gi|669144700|ref|XP_008607912.1| (hypothetical protein SDRG_04039 [Saprolegnia diclina VS20] >gi|530740078|gb|EQC38320.1| hypothetical protein SDRG_04039 [Saprolegnia diclina VS20])

HSP 1 Score: 183.341 bits (464), Expect = 2.723e-52
Identity = 89/210 (42.38%), Postives = 129/210 (61.43%), Query Frame = 0
Query:  115 LLRKQRREQRKNQRHEAQMAFLRRCEEGARVVIDCGFDSHMTEKELKSLGQQLMYVYASNKRALAPCTVHIAELGAGIQQRLSCVAGVQNWQGFNRETRPFMETYAAERDKLVYLTSDSPNVLTQLDPGLIYVIGGIVDRNRLKHATYNKALAQGIATARFPLREHVQLG-SSPVLAVNHVFDILLEFQYTNDWRATFAKVLPNRKSVKV 323
            L+RK+R   ++        +FL    EG  +VIDCGF+  MT KE KSL QQ+M+ Y  NKR+  P  VH+  +    +  L+ + G  +W  F+   + +M+ +   ++ LVYLT+DSPNV+ +L    +Y+IGGIVDRNRLK  TY KA+AQGIATA+ PL + +++G ++ VL VNHVF IL+ ++   DW       LP RK  +V
Sbjct:   84 LMRKERAIAKRE-------SFLMAASEGPTIVIDCGFEEAMTSKEKKSLTQQIMFSYGINKRSETPANVHVTSVRGEAEANLTKIGGFNDWLAFSTTPKCYMDVF--RKESLVYLTADSPNVIDELQSDKVYIIGGIVDRNRLKGVTYEKAVAQGIATAKLPLDKVLEMGQATRVLTVNHVFQILVNYRTAQDWTQATLSALPARKGAQV 284          
BLAST of EWM28782.1 vs. NCBI_GenBank
Match: gi|813218974|ref|XP_012210864.1| (hypothetical protein SPRG_16203 [Saprolegnia parasitica CBS 223.65] >gi|641523656|gb|KDO18434.1| hypothetical protein SPRG_16203 [Saprolegnia parasitica CBS 223.65])

HSP 1 Score: 182.185 bits (461), Expect = 6.941e-52
Identity = 88/210 (41.90%), Postives = 129/210 (61.43%), Query Frame = 0
Query:  115 LLRKQRREQRKNQRHEAQMAFLRRCEEGARVVIDCGFDSHMTEKELKSLGQQLMYVYASNKRALAPCTVHIAELGAGIQQRLSCVAGVQNWQGFNRETRPFMETYAAERDKLVYLTSDSPNVLTQLDPGLIYVIGGIVDRNRLKHATYNKALAQGIATARFPLREHVQLG-SSPVLAVNHVFDILLEFQYTNDWRATFAKVLPNRKSVKV 323
            L+RK+R   ++        +FL    EG  +VIDCGF+  M+ KE KSL QQ+M+ Y  NKR+  P  VH+  +    +  L+ + G  +W  F+   + +M+ +   ++ LVYLT+DSPNV+ +L    +Y+IGGIVDRNRLK  TY KA+AQGIATA+ PL + +++G ++ VL VNHVF IL+ ++   DW       LP RK  +V
Sbjct:   75 LMRKERAIAKRE-------SFLMAASEGPTIVIDCGFEEAMSSKEKKSLTQQIMFSYGINKRSETPANVHVTSVRGEAEANLTKIGGFNDWLAFSTTPKCYMDVF--RKESLVYLTADSPNVIDELQSDKVYIIGGIVDRNRLKGVTYEKAVAQGIATAKLPLDKVLEMGQATRVLTVNHVFQILVNYRTAQDWTQATLSALPARKGAQV 275          
BLAST of EWM28782.1 vs. NCBI_GenBank
Match: gi|301117816|ref|XP_002906636.1| (RNA (guanine-9-)-methyltransferase domain-containing protein, putative [Phytophthora infestans T30-4] >gi|262107985|gb|EEY66037.1| RNA (guanine-9-)-methyltransferase domain-containing protein, putative [Phytophthora infestans T30-4])

HSP 1 Score: 180.644 bits (457), Expect = 1.213e-51
Identity = 89/203 (43.84%), Postives = 127/203 (62.56%), Query Frame = 0
Query:  120 RREQRKNQRHEAQMAFLRRCEEGARVVIDCGFDSHMTEKELKSLGQQLMYVYASNKRALAPC--TVHIAELGAGIQQRLSCVAGVQNWQGFNRETRPFMETYAAERDKLVYLTSDSPNVLTQLDPGLIYVIGGIVDRNRLKHATYNKALAQGIATARFPLREHVQLGSSP-VLAVNHVFDILLEFQYTNDWRATFAKVLPNRK 319
            RR++R N + EA   +L   EEG +VVIDC F+  MT+KE KSL QQ+M+ Y  N+++  P    V+I  L    +  L  + G   W  F   ++ +M+ +  +++ LVYLT+DSPN++T+L    +Y+IGGIVDRNRLK  TY KA+ QGI TA+ PL   V++G++  VL VNHVF+IL +F    DW       LP+RK
Sbjct:   49 RRKERANAKREA---YLMAAEEGVKVVIDCEFEEKMTDKEKKSLSQQIMFSYGVNRKSRTPMKYVVYITSLHGDTRNNLENICGFHEWLAFTESSKSYMDFF--KKESLVYLTADSPNIITKLSRDKVYIIGGIVDRNRLKGITYEKAVEQGIETAKLPLDAVVEMGAATRVLTVNHVFEILAQFSEIKDWAQATLATLPSRK 246          
BLAST of EWM28782.1 vs. NCBI_GenBank
Match: gi|1013946622|gb|KYR00297.1| (putative RNaseIII [Dictyostelium lacteum])

HSP 1 Score: 183.341 bits (464), Expect = 1.587e-51
Identity = 101/273 (37.00%), Postives = 152/273 (55.68%), Query Frame = 0
Query:   56 VSKSVAKKLWRQEMWKVKKATKKAAKRAARQETNRQSGEVGQDDNLNLQAATSESKNDQLLRKQRREQRKNQRHEAQMAFLRRCEEGARVVIDCGFDSHMTEKELKSLGQQLMYVYASNKRALAPCTVHIAELGAGIQQRLSCVAGVQNWQGFNRETRPFMETYAAER-DKLVYLTSDSPNVLTQLDPGLIYVIGGIVDRNRLKHATYNKALAQGIATARFPLREHVQLGSSPVLAVNHVFDILLEFQYTNDWRATFAKVLPNRKSVKVNEPA 327
            +SK+  KKL R E W  +KA KK   +  +++  ++  E+   +++N  A  +               +K +R  + + +         V+ID      M E+E KSL  Q+ ++Y +N+ A  P  + ++   + I+ +   + G +NW G +R ++ + + Y+ +R   LVYL+SDSPNV+T LDP  IY+IGG+VD NRLK  TYNKA  QGI TA+ P+ E +QL S  VLAVNHVFDIL  +   NDW+  F  V+P R   K NE A
Sbjct:   46 LSKNQQKKLIRSEKWAKQKAEKKDELKQRQKDNKKKRKEMMTPEDINKLAEIN---------------KKKKRDPSHLQY------SGTVLIDLEECDKMAEREQKSLVTQVQFLYGTNRLAEKPFHIELSGFQSQIKDKFERMVGFENWTGIDRYSQSYFDKYSKDRLQDLVYLSSDSPNVITTLDPSKIYIIGGLVDHNRLKGLTYNKATTQGIYTAKLPISEFIQLQSRKVLAVNHVFDILSHYTECNDWKKAFETVIPLR---KFNESA 294          
BLAST of EWM28782.1 vs. NCBI_GenBank
Match: gi|1109210024|ref|XP_019179453.1| (PREDICTED: tRNA (guanine(9)-N1)-methyltransferase isoform X3 [Ipomoea nil])

HSP 1 Score: 183.726 bits (465), Expect = 2.514e-51
Identity = 115/340 (33.82%), Postives = 177/340 (52.06%), Query Frame = 0
Query:    1 MEPKRSANSAAEAVRDASEDTLSIP------APPSASAPSSSTPVKGGNLFLFRKPSRVKDVSKSVAKKLWRQEMWKVKKATKKAAKRAARQ-ETNRQSGEVGQD-DNLNLQAATSESKNDQLLRKQRREQRKNQRHEAQMAFLRRCEEGARVVIDCGFDSHMTEKELKSLGQQLMYVYASNKRALAPCTVHIAELGAGIQQRLSCVAGVQNWQGFNRETRPFMETYAAERDKLVYLTSDSPNVLTQLDPGLIYVIGGIVDRNRLKHATYNKALAQGIATARFPLREHVQLGSSPVLAVNHVFDILLEFQYTNDWRATFAKVLPNRKSVKVNEPAVNKDS 332
            + P  S++    A+   S+D  S P       PP+ SAP    P+                 SK   KKL +Q+ ++ KKA KKA  +  +Q E  R+  E  +    ++ +      +  + +R++R E+R  ++ +      R  E G   +ID  F   MT  EL SL QQ+MY YA N R  +P  + +A     +Q  L    G   W    +E+RP++E +  +++ LVYLT+DS NVL +LDP  +Y+IGG+VDRNR K  T  KA  QGI TA+ P+  ++++ SS VL VN V +ILL+F    DW+A+F +V+P RK    +   V+ ++
Sbjct:   32 LHPFASSHCLLVAMESNSDDHQSPPLATATSPPPATSAPDQQPPL-----------------SKRAQKKLLKQQRFEAKKAEKKAQLKEHKQREAERKRREWAERLAGMSEEEKLKLIEERKSVRRERMEKRSEEKGKKIERLTRAKESGQNFIIDLEFSDLMTSNELHSLVQQIMYCYAVNGRCASPAHLWLAGYQGEMQNLLEKTPGYDKWL-IEKESRPYIEAFEDQKEDLVYLTADSVNVLDELDPKKLYIIGGLVDRNRWKGITMKKAEEQGIQTAKLPIGNYLKMSSSQVLTVNQVVEILLKFLELRDWKASFFEVIPQRKRCGADSEDVDDEA 353          
The following BLAST results are available for this feature:
BLAST of EWM28782.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10
Match NameE-valueIdentityDescription
gi|585111205|gb|EWM28782.1|0.000e+0100.00rna (guanine-9-)-methyltransferase domain-containi... [more]
gi|1214710777|gb|OWZ21837.1|4.449e-5638.01RNA (guanine-9-)-methyltransferase [Phytophthora m... [more]
gi|1173949772|gb|OQR97776.1|7.930e-5638.75RNA (guanine-9-)-methyltransferase domain-containi... [more]
gi|953499128|emb|CEG39440.1|7.823e-5445.50rna (guanine-9-)-methyltransferase domain-containi... [more]
gi|695376083|ref|XP_009515698.1|3.006e-5340.22hypothetical protein PHYSODRAFT_477148 [Phytophtho... [more]
gi|669144700|ref|XP_008607912.1|2.723e-5242.38hypothetical protein SDRG_04039 [Saprolegnia dicli... [more]
gi|813218974|ref|XP_012210864.1|6.941e-5241.90hypothetical protein SPRG_16203 [Saprolegnia paras... [more]
gi|301117816|ref|XP_002906636.1|1.213e-5143.84RNA (guanine-9-)-methyltransferase domain-containi... [more]
gi|1013946622|gb|KYR00297.1|1.587e-5137.00putative RNaseIII [Dictyostelium lacteum][more]
gi|1109210024|ref|XP_019179453.1|2.514e-5133.82PREDICTED: tRNA (guanine(9)-N1)-methyltransferase ... [more]
back to top
Relationships

This CDS is a part of the following mRNA feature(s):

Feature NameUnique NameSpeciesType
rna1970rna1970Nannochloropsis gaditana (N. gaditana B-31)mRNA


Sequences
Synonyms
Publications