NO25G01780, NO25G01780 (gene) Nannochloropsis oceanica

Overview
NameNO25G01780
Unique NameNO25G01780
Typegene
OrganismNannochloropsis oceanica (N. oceanica IMET1)
Sequence length5538
Alignment locationchr25:528933..534470 +

Link to JBrowse

Properties
Property NameValue
DescriptionGlutamate cysteine ligase
Mutants
Expression

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
chr25genomechr25:528933..534470 +
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
PRJNA7699582024-08-13
GSE1786722023-12-15
PRJNA9336932023-10-26
PRJNA9434492023-07-19
GSE1499042020-10-10
NoIMET1_WT_HS2020-09-29
GSE1396152020-03-11
PRJNA2413822020-03-11
BLASTP analysis of N. oceanica IMET1 genes2019-07-11
InterPro analysis for N. oceanica IMET1 genes2019-07-11
PRJNA1821802019-04-25
Gene prediction for N. oceanica IMET12017-10-24
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0004357glutamate-cysteine ligase activity
Vocabulary: Biological Process
TermDefinition
GO:0006750glutathione biosynthetic process
Vocabulary: INTERPRO
TermDefinition
IPR014746Gln_synth/guanido_kin_cat_dom
IPR004308GCS
Homology
BLAST of NO25G01780 vs. NCBI_GenBank
Match: XP_002297305.1 (glutamate-cysteine ligase, partial [Thalassiosira pseudonana CCMP1335] >EED86407.1 glutamate-cysteine ligase, partial [Thalassiosira pseudonana CCMP1335])

HSP 1 Score: 738.4 bits (1905), Expect = 2.300e-209
Identity = 383/749 (51.13%), Postives = 486/749 (64.89%), Query Frame = 0
Query:   23 GLLKVGRPLPWHVSLPHLRPVRRAGVRQFLNIYHRLKDIRQDGLLWGEEVEYGIFKVDRSKRQVWLSLRGKEVMESLIKKEQDYTQRSEGCSWHPEFGSWMVESTPSRPYAGYATDLLRVESNMRLRRKRLLTSLDKDEIAPTVVAFPLMGV--GDFVDPPAPPGGPVSDSAFVPDSCINPHPRFGTLVKNIRTRRGSKVDIRVPLFKDLDTPEFKAWDEITRRVGAPRPYINMDAMAFGMGCCCLQVTFQARDIDESRYMHDQLAVLAPILMALTAASPIFKGRLADTDARWDTIAGAVDDRTPAERGELKGEALTAAANPLMAGEGIQRLFKSRYGSISTFIYSCGDEEQQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTITTEKEKRKTSAIDRYNDIPCEVDKELQAMLLEGGVDPALSRHVAHLFARDPLVIFDDAI-EVDDSKTTEHFENIQSTNWQTVRWKPPPQRTTPSDPHIGWRTEFRSMEIQLTDFENAAFTVFVILLTRVILTFDLSLYLPLSKVDDNMAKAHQRDGLLKEKFWFRKHLAPXXXXXXXXXXXXXXXXXXDKAFEGAQKAGFVASSSCQDHVWQGTADEYELMTVEEILMGKGDYFLGLIPLCNMYLDYIGCDRETFRRVSVYLDFIARRGAGETMTTAAWMRQFVTKHPEYKRDSVVSPSIAYDLMMECKRIGEGRLQCPEVMGNVQIDPIFPGEAYDVYLSGTKMGSSERAELVKAYRKRGPF 769
            GLLKVG P PW  S  +L  VR AGVRQF++ Y+R+KD++ D L+WG+E+EYGIF +D   ++V LSLR KE+M++L   E  ++  +EGC+W PE+G+WMVE+TP+RPY GY+TDLLRVE NMRLRRKR+L  L + EIAPTV  FPL+G    D   PP P GGPV++S ++ D  INPHPRFG L  NIR RRG KV+I VPLF D  TPE+       +     RP I+MDAMAFGMGCCCLQ+TFQA D++ESR++ DQLAV+API+MALTA++P+ KGRLADTD RW  I+  VDDRTPAERG   G       +  MAG+G +RL+KSRY SIST++Y                                         +TE    K   ++ YNDIP  +D++    L E G+DP LS+H+AH+F RDPLVIFD A+ EVDD   TEHFE+IQSTNWQTVRWKPPP R+ P+DPHIGWRTEFRSMEIQLTDFENAAFT F  LLTRVILTFDL+LY+PLS+VD NM +AH R+  +K KF+F                               ++    AS   ++       + YE MT+ EI+ GKGDYF GLIPL   YLD+I CD  T  R++ YLDFI +R  G+ +T A W+R F+  H +YK DSVVS SIAYDL++ CK IGEG+   PE++G+V I PI   +AY   L   ++ + +   L++ Y  R  F
Sbjct:    1 GLLKVGTPQPWPTSKRNLNYVREAGVRQFISTYNRVKDLKGDDLVWGDEIEYGIFVLDDENKKVRLSLRAKEIMDNLNANEGIHSHEAEGCNWVPEYGAWMVEATPARPYTGYSTDLLRVERNMRLRRKRILAELKEGEIAPTVAVFPLLGAMEDDGSVPPTPLGGPVTESEYIGDGIINPHPRFGALSANIRKRRGEKVNICVPLFHDTHTPEYANLPGFAQMFNV-RPAIHMDAMAFGMGCCCLQITFQATDVEESRFLFDQLAVMAPIMMALTASTPVLKGRLADTDCRWGIISEGVDDRTPAERGREGGP--DPPGDIEMAGKGRRRLYKSRYDSISTYLYQ--------------------------------------GASTEDSHMKNRILNMYNDIPVPIDEDKYRDLREAGIDPLLSQHIAHIFVRDPLVIFDGAVEEVDDETQTEHFESIQSTNWQTVRWKPPPPRSGPNDPHIGWRTEFRSMEIQLTDFENAAFTTFTTLLTRVILTFDLNLYIPLSRVDANMQRAHGRNAAVKGKFFFH-------------------------GVSAKRRVAPNASGGPEE-------NSYEEMTMAEIMTGKGDYFPGLIPLVYAYLDHIQCDSVTMERMTKYLDFIEKRATGQLVTPATWVRSFIRNHEDYKFDSVVSDSIAYDLLVACKEIGEGQRAAPELLGDVVIKPILATDAYQKKLDSKRVNNGQILSLLERYSARKTF 676          
BLAST of NO25G01780 vs. NCBI_GenBank
Match: XP_002179736.1 (predicted protein [Phaeodactylum tricornutum CCAP 1055/1] >EEC48722.1 predicted protein [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 731.5 bits (1887), Expect = 2.900e-207
Identity = 383/764 (50.13%), Postives = 503/764 (65.84%), Query Frame = 0
Query:   23 GLLKVGRPLPWHVSLPHLRPVRRAGVRQFLNIYHRLKDIRQDGLLWGEEVEYGIFKVDRSKRQVWLSLRGKEVMESLIKKEQDYTQRSEGCSWHPEFGSWMVESTPSRPYAGYATDLLRVESNMRLRRKRLLTSLDKDEIAPTVVAFPLMGV--GDFVDPPAPPGGPVSDSAFVPDSCINPHPRFGTLVKNIRTRRGSKVDIRVPLFKDLDTPEFKAWD--------EITRRVGAPRPYINMDAMAFGMGCCCLQVTFQARDIDESRYMHDQLAVLAPILMALTAASPIFKGRLADTDARWDTIAGAVDDRTPAERGELKGEALTAAANPLMAGEGIQRLFKSRYGSISTFIYSCGDEEQQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTITTEKEKRKTSAIDR----YNDIPCEVDKELQAMLLEGGVDPALSRHVAHLFARDPLVIFDDAI-EVDDSKTTEHFENIQSTNWQTVRWKPPPQRTTPSDPHIGWRTEFRSMEIQLTDFENAAFTVFVILLTRVILTFDLSLYLPLSKVDDNMAKAHQRDGLLKEKFWFRKHLAPXXXXXXXXXXXXXXXXXXDKAFEGAQKAGFVASSSCQDHVWQGTADEYELMTVEEILMGKGDYFLGLIPLCNMYLDYIGCDRETFRRVSVYLDFIARRGAGETMTTAAWMRQFVTKHPEYKRDSVVSPSIAYDLMMECKRIGEGRLQCPEVMGNVQIDPIFPGEAYDVYLSGTKMGSSERAELVKAYRKRGPFHAN 772
            GLLKVG P  W  S   L  +R+AGVRQF++ Y+R+KD + D LLWG+E+EYGIF +D + ++V LSLR KE+M+ L +KE  ++Q  EGC+W PE+G+WMVE+TP RPY GY TDLLRVE NMRLRRKR+LT L ++E+APT+ +F ++G    D   P    GGPV++S ++ D  INPHPRFGTL  NIR RRGSKV+IRVPLF+D  TPE+  ++        E  R     R  I+MDAMAFGMGCCCLQ+TFQA+DI+ESR+++DQLAV+API+MALTA++PI KGRLADTDARW  I+ +VD RT AERG    +A     N     +G +R++KSRY SIST+IY                                        + + K+++ T   +R    YNDIP  +D++    L + G+DPAL++HVAHLF RDPLV+F+ A+ EVDD   TEH+E+IQSTNWQ+VRWKPPP R +P+DPHIGWRTEFRSME+QLTDFENAAFT F++LLTRVILTFDL+LY+P+S+VD NM +AH R+   K KF+FR+H+AP                      EG    G                + +E MT+ EI+ GKGD+F GLI L   YLD+I CD+ T +R+  YL FIA+R  GE +T A WMR FV  HP Y++DS++S  IAYDLM+ C+ IG+G+L  PE++G+  IDPI    AYDV L   ++ +    +L++ Y KR  F A+
Sbjct:   48 GLLKVGVPKSWDDSKKDLDYIRKAGVRQFVSTYNRVKDFKGDELLWGDEIEYGIFHMDHNAKKVRLSLRAKEIMDELNRKETLHSQIVEGCNWVPEYGAWMVEATPDRPYTGYTTDLLRVERNMRLRRKRMLTVLSEEEVAPTISSFFMLGAQGDDGTIPSTTVGGPVTESEYISDGIINPHPRFGTLTANIRQRRGSKVNIRVPLFRDTRTPEYIDFEPPKGSEIPEEMRSDETCRASIHMDAMAFGMGCCCLQITFQAKDIEESRFIYDQLAVMAPIMMALTASTPILKGRLADTDARWGIISESVDCRTLAERGREDPDAPYEDYN----RKGQRRIYKSRYDSISTYIYQ--------------------------------------GVLSLKDEKSTGLANRVLNIYNDIPVPIDEDSYKQLRDAGIDPALAQHVAHLFIRDPLVVFNGAVEEVDDDTQTEHWESIQSTNWQSVRWKPPPPRNSPNDPHIGWRTEFRSMELQLTDFENAAFTAFIVLLTRVILTFDLNLYIPMSRVDANMQRAHSRNSAAKGKFFFRRHMAPLQ--------------------EGDDGYGV-----------NSEENSFEEMTMNEIMNGKGDHFPGLIVLILAYLDHINCDKVTLKRLENYLTFIAKRATGELVTPATWMRNFVRNHPAYEQDSILSDEIAYDLMIACRDIGQGKLHVPELLGDNIIDPITSEGAYDVKLDSKRVQNDRILDLLRQYTKRKSFSAH 738          
BLAST of NO25G01780 vs. NCBI_GenBank
Match: OEU14148.1 (gamma glutamylcysteine synthetase [Fragilariopsis cylindrus CCMP1102])

HSP 1 Score: 729.9 bits (1883), Expect = 8.300e-207
Identity = 388/768 (50.52%), Postives = 502/768 (65.36%), Query Frame = 0
Query:   23 GLLKVGRPLPWHVSLPHLRPVRRAGVRQFLNIYHRLKDIRQDGLLWGEEVEYGIFKVDRSKRQVWLSLRGKEVMESLIKKEQDYTQRSEGCSWHPEFGSWMVESTPSRPYAGYATDLLRVESNMRLRRKRLLTSLDKDEIAPTVVAFPLMGV--GDFVDPPAPPGGPVSDSAFVPDSCINPHPRFGTLVKNIRTRRGSKVDIRVPLFKDLDTPEFKAWDEITRRVGA-------PRPYINMDAMAFGMGCCCLQVTFQARDIDESRYMHDQLAVLAPILMALTAASPIFKGRLADTDARWDTIAGAVDDRTPAERGELKGEALTAAANPLMAGEGIQRLFKSRYGSISTFIYSCGDEEQQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTITTEKEKRKTSAIDRYNDIPCEVDKELQAMLLEGGVDPALSRHVAHLFARDPLVIFDDAI-EVDDSKTTEHFENIQSTNWQTVRWKPPPQRTTPSDPHIGWRTEFRSMEIQLTDFENAAFTVFVILLTRVILTFDLSLYLPLSKVDDNMAKAHQRDGLLKEKFWFRKHLAP---------XXXXXXXXXXXXXXXXXXDKAFEGAQKAGFVASSSCQDHVWQGTADE--YELMTVEEILMGKGDYFLGLIPLCNMYLDYIGCDRETFRRVSVYLDFIARRGAGETMTTAAWMRQFVTKHPEYKRDSVVSPSIAYDLMMECKRIGEGRLQCPEVMGNVQIDPIFPGEAYDVYLSGTKMGSSERAELVKAYRKR-GPF 769
            GLLKVG P  W  S   L  +R+AGVRQF++ Y+R+KD++ D LLWG+EVEYGIF +D   ++V LSLR KEVM+ L +KE  ++Q SEGC+W PE+G+WMVE+TPSRPY G++TDLLRVE NMRLRRKR+LT L+++EIAPTV  FPL+G    D   PP   GGP+++S ++ D  INPHPRFGTL  NIR RRGSKV+I++PLF+D DT EFK  D     V          R  +++DAMAFGMGCCCLQ+TFQA+D+DESR++ DQLAV+API+MALTA++PI KGRL DTDARW  I+ +VD RT +ER    G A   A +      G ++++KSRY SIST+IY                                       T   + +      ++ YNDIP  +D+     L E G+DPAL++HVAHLF RDPLVIF+ A+ EVDD   TEHFE+IQSTNWQTVRWKPPP R +P+ PHIGWRTEFRSMEIQLTDFENAAF+VF +LL R ILTFDL+LY+P+SKVD NM +AH R+   K KF+FR+HLAP                            + FE A+ A  V++        +G+A+E  +E MT+EEI+ GK D++ GLIPL   YLD+I  D  +  R+  YLDF+ +R  GE +TTA W+R FV  HP+YK+DS V+  IA+DL++  K IGEG+L  PE++G+VQI  I    AY   L   ++ +    EL++ Y KR  PF
Sbjct:    2 GLLKVGVPKSWDDSKKDLEYIRKAGVRQFISTYNRVKDLKGDELLWGDEVEYGIFHIDPETKKVRLSLRAKEVMDELNRKEAVHSQISEGCNWVPEYGAWMVEATPSRPYTGFSTDLLRVERNMRLRRKRILTVLNENEIAPTVSVFPLLGCLGDDGSVPPTAVGGPITESEYIGDGIINPHPRFGTLTANIRQRRGSKVNIKIPLFRDTDTQEFKELDMPKSGVDGYNNSGDMLRAAVHVDAMAFGMGCCCLQITFQAKDVDESRFIFDQLAVMAPIMMALTASTPILKGRLLDTDARWGIISESVDGRTLSER----GRADPNAPHDYYNANGQRQIYKSRYDSISTYIYQ-----------------------------------GSMTGGQDAKDLSNRVLNIYNDIPLAIDESCYQDLREAGIDPALAQHVAHLFIRDPLVIFNGAVDEVDDETQTEHFESIQSTNWQTVRWKPPPPRDSPNAPHIGWRTEFRSMEIQLTDFENAAFSVFTVLLQRTILTFDLNLYIPISKVDANMQRAHSRNSAAKGKFFFRRHLAPLEEGDDGYGVKYVSMFSQGVKGAYNETSENFE-AEDADGVSNYRKSAPTARGSAEENAFEEMTMEEIMCGKDDHYPGLIPLVRAYLDHISADSTSMARLHTYLDFVEKRSTGELITTATWVRNFVRNHPDYKQDSHVTEKIAHDLLVASKEIGEGKLHAPELLGDVQIQEISTDGAYSTKLDSRRVTNQRIVELLQRYTKRQSPF 729          
BLAST of NO25G01780 vs. NCBI_GenBank
Match: OEU14149.1 (gamma glutamylcysteine synthetase [Fragilariopsis cylindrus CCMP1102])

HSP 1 Score: 729.9 bits (1883), Expect = 8.300e-207
Identity = 388/768 (50.52%), Postives = 502/768 (65.36%), Query Frame = 0
Query:   23 GLLKVGRPLPWHVSLPHLRPVRRAGVRQFLNIYHRLKDIRQDGLLWGEEVEYGIFKVDRSKRQVWLSLRGKEVMESLIKKEQDYTQRSEGCSWHPEFGSWMVESTPSRPYAGYATDLLRVESNMRLRRKRLLTSLDKDEIAPTVVAFPLMGV--GDFVDPPAPPGGPVSDSAFVPDSCINPHPRFGTLVKNIRTRRGSKVDIRVPLFKDLDTPEFKAWDEITRRVGA-------PRPYINMDAMAFGMGCCCLQVTFQARDIDESRYMHDQLAVLAPILMALTAASPIFKGRLADTDARWDTIAGAVDDRTPAERGELKGEALTAAANPLMAGEGIQRLFKSRYGSISTFIYSCGDEEQQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTITTEKEKRKTSAIDRYNDIPCEVDKELQAMLLEGGVDPALSRHVAHLFARDPLVIFDDAI-EVDDSKTTEHFENIQSTNWQTVRWKPPPQRTTPSDPHIGWRTEFRSMEIQLTDFENAAFTVFVILLTRVILTFDLSLYLPLSKVDDNMAKAHQRDGLLKEKFWFRKHLAP---------XXXXXXXXXXXXXXXXXXDKAFEGAQKAGFVASSSCQDHVWQGTADE--YELMTVEEILMGKGDYFLGLIPLCNMYLDYIGCDRETFRRVSVYLDFIARRGAGETMTTAAWMRQFVTKHPEYKRDSVVSPSIAYDLMMECKRIGEGRLQCPEVMGNVQIDPIFPGEAYDVYLSGTKMGSSERAELVKAYRKR-GPF 769
            GLLKVG P  W  S   L  +R+AGVRQF++ Y+R+KD++ D LLWG+EVEYGIF +D   ++V LSLR KEVM+ L +KE  ++Q SEGC+W PE+G+WMVE+TPSRPY G++TDLLRVE NMRLRRKR+LT L+++EIAPTV  FPL+G    D   PP   GGP+++S ++ D  INPHPRFGTL  NIR RRGSKV+I++PLF+D DT EFK  D     V          R  +++DAMAFGMGCCCLQ+TFQA+D+DESR++ DQLAV+API+MALTA++PI KGRL DTDARW  I+ +VD RT +ER    G A   A +      G ++++KSRY SIST+IY                                       T   + +      ++ YNDIP  +D+     L E G+DPAL++HVAHLF RDPLVIF+ A+ EVDD   TEHFE+IQSTNWQTVRWKPPP R +P+ PHIGWRTEFRSMEIQLTDFENAAF+VF +LL R ILTFDL+LY+P+SKVD NM +AH R+   K KF+FR+HLAP                            + FE A+ A  V++        +G+A+E  +E MT+EEI+ GK D++ GLIPL   YLD+I  D  +  R+  YLDF+ +R  GE +TTA W+R FV  HP+YK+DS V+  IA+DL++  K IGEG+L  PE++G+VQI  I    AY   L   ++ +    EL++ Y KR  PF
Sbjct:   43 GLLKVGVPKSWDDSKKDLEYIRKAGVRQFISTYNRVKDLKGDELLWGDEVEYGIFHIDPETKKVRLSLRAKEVMDELNRKEAVHSQISEGCNWVPEYGAWMVEATPSRPYTGFSTDLLRVERNMRLRRKRILTVLNENEIAPTVSVFPLLGCLGDDGSVPPTAVGGPITESEYIGDGIINPHPRFGTLTANIRQRRGSKVNIKIPLFRDTDTQEFKELDMPKSGVDGYNNSGDMLRAAVHVDAMAFGMGCCCLQITFQAKDVDESRFIFDQLAVMAPIMMALTASTPILKGRLLDTDARWGIISESVDGRTLSER----GRADPNAPHDYYNANGQRQIYKSRYDSISTYIYQ-----------------------------------GSMTGGQDAKDLSNRVLNIYNDIPLAIDESCYQDLREAGIDPALAQHVAHLFIRDPLVIFNGAVDEVDDETQTEHFESIQSTNWQTVRWKPPPPRDSPNAPHIGWRTEFRSMEIQLTDFENAAFSVFTVLLQRTILTFDLNLYIPISKVDANMQRAHSRNSAAKGKFFFRRHLAPLEEGDDGYGVKYVSMFSQGVKGAYNETSENFE-AEDADGVSNYRKSAPTARGSAEENAFEEMTMEEIMCGKDDHYPGLIPLVRAYLDHISADSTSMARLHTYLDFVEKRSTGELITTATWVRNFVRNHPDYKQDSHVTEKIAHDLLVASKEIGEGKLHAPELLGDVQIQEISTDGAYSTKLDSRRVTNQRIVELLQRYTKRQSPF 770          
BLAST of NO25G01780 vs. NCBI_GenBank
Match: XP_002291923.1 (glutamate-cysteine ligase, partial [Thalassiosira pseudonana CCMP1335] >EED90774.1 glutamate-cysteine ligase, partial [Thalassiosira pseudonana CCMP1335])

HSP 1 Score: 726.9 bits (1875), Expect = 7.100e-206
Identity = 374/709 (52.75%), Postives = 469/709 (66.15%), Query Frame = 0
Query:   23 GLLKVGRPLPWHVSLPHLRPVRRAGVRQFLNIYHRLKDIRQDGLLWGEEVEYGIFKVDRSKRQVWLSLRGKEVMESLIKKEQDYTQRSEGCSWHPEFGSWMVESTPSRPYAGYATDLLRVESNMRLRRKRLLTSLDKDEIAPTVVAFPLMGV--GDFVDPPAPPGGPVSDSAFVPDSCINPHPRFGTLVKNIRTRRGSKVDIRVPLFKDLDTPEFKAWDEITRRVGAPRPYINMDAMAFGMGCCCLQVTFQARDIDESRYMHDQLAVLAPILMALTAASPIFKGRLADTDARWDTIAGAVDDRTPAERGELKGEALTAAANPLMAGEGIQRLFKSRYGSISTFIYSCGDEEQQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTITTEKEKRKTSAIDRYNDIPCEVDKELQAMLLEGGVDPALSRHVAHLFARDPLVIFDDAI-EVDDSKTTEHFENIQSTNWQTVRWKPPPQRTTPSDPHIGWRTEFRSMEIQLTDFENAAFTVFVILLTRVILTFDLSLYLPLSKVDDNMAKAHQRDGLLKEKFWFRKHLAPXXXXXXXXXXXXXXXXXXDKAFEGAQKAGFVASSSCQDHVWQGTADEYELMTVEEILMGKGDYFLGLIPLCNMYLDYIGCDRETFRRVSVYLDFIARRGAGETMTTAAWMRQFVTKHPEYKRDSVVSPSIAYDLMMECKRIGEGRLQCPEVMG 729
            GLLKVG P PW  S  +L  VR AGVRQF++ Y+R+KD++ D L+WG+E+EYGIF +D   ++V LSLR KE+M++L   E  ++  +EGC+W PE+G+WMVE+TP+RPY GY+TDLLRVE NMRLRRKR+L  L + EIAPTV  FPL+G    D   PP P GGPV++S ++ D  INPHPRFG L  NIR RRG KV+IRVPLF D  TPE+       +     RP I+MDAMAFGMGCCCLQ+TFQA D++ESR++ DQLAV+API+MALTA++P+ KGRLADTD RW  I+  VDDRTPAERG   G       +P MAG+G +RL+KSRY SIST++Y                                         +TE    K   ++ YNDIP  +D++    L E G+DP LS+H+AH+F RDPLVIFD A+ EVDD   TEHFE+IQSTNWQTVRWKPPP R+ P+DPHIGWRTEFRSMEIQLTDFENAAFT F  LLTRVILTFDL+LY+PLS+VD NM +AH R+  +K KF+F                               ++    AS   ++       + YE MT+ EI+ GKGDYF GLIPL   YLD+I CD  T  R++ YLDFI +R  G+ +T A W+R F+  H +YK DSVVS SIAYDL++ CK IGEG+   PE++G
Sbjct:    1 GLLKVGTPQPWPTSKRNLNYVREAGVRQFISTYNRVKDLKGDDLVWGDEIEYGIFVLDDENKKVRLSLRAKEIMDNLNANEGIHSHEAEGCNWVPEYGAWMVEATPARPYTGYSTDLLRVERNMRLRRKRILAELKEGEIAPTVAVFPLLGAMEDDGSVPPTPLGGPVTESEYIGDGIINPHPRFGALSANIRKRRGEKVNIRVPLFHDTHTPEYANLPGFAQMFNV-RPAIHMDAMAFGMGCCCLQITFQATDVEESRFLFDQLAVMAPIMMALTASTPVLKGRLADTDCRWGIISEGVDDRTPAERGREGGP--DPPGDPEMAGKGRRRLYKSRYDSISTYLYQ--------------------------------------GASTEDSHMKNRILNMYNDIPVPIDEDKYRDLREAGIDPLLSQHIAHIFVRDPLVIFDGAVEEVDDETQTEHFESIQSTNWQTVRWKPPPPRSGPNDPHIGWRTEFRSMEIQLTDFENAAFTTFTTLLTRVILTFDLNLYIPLSRVDANMQRAHGRNAAVKGKFFFH-------------------------GVSAKRRVAPNASGGPEE-------NSYEEMTMAEIMTGKGDYFPGLIPLVYAYLDHIQCDSVTMERMTKYLDFIEKRATGQLVTPATWVRSFIRNHEDYKFDSVVSDSIAYDLLVACKEIGEGQRAAPELLG 636          
BLAST of NO25G01780 vs. NCBI_GenBank
Match: CBJ30287.1 (Glutamate cysteine ligase [Ectocarpus siliculosus])

HSP 1 Score: 721.8 bits (1862), Expect = 2.300e-204
Identity = 392/785 (49.94%), Postives = 498/785 (63.44%), Query Frame = 0
Query:   12 LLILSPSPTEAGLLKVGRPLPWHVSLPHLRPVRRAGVRQFLNIYHRLKDIRQDGLLWGEEVEYGIFKVDRSKRQVWLSLRGKEVMESLIKKEQDYTQRSEGCSWHPEFGSWMVESTPSRPYAGYATDLLRVESNMRLRRKRLLTSLDKDEIAPTVVAFPLMGVGDFVDPPAPPGGPVSDSAFVPDSCINPHPRFGTLVKNIRTRRGSKVDIRVPLFKDLDTPEFKAWDEITRRV-GAPRPYINMDAMAFGMGCCCLQVTFQARDIDESRYMHDQLAVLAPILMALTAASPIFKGRLADTDARWDTIAGAVDDRT-----PAER---GELKGEALTA----AANPLMAGEGIQRLFKSRYGSISTFIYSCGDEEQQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTITTEKEKRKTSAIDRYNDIPCEVDKELQAMLLEGGVDPALSRHVAHLFARDPLVIFDDAI-EVDDSKTTEHFENIQSTNWQTVRWKPPPQRTTPSDPHIGWRTEFRSMEIQLTDFENAAFTVFVILLTRVILTFDLSLYLPLSKVDDNMAKAHQRDGLLKEKFWFRKHLAPXXXXXXXXXXXXXXXXXXDKAFEGAQKAGFVASSSCQDHVWQGTADEYELMTVEEILMGKGDYFLGLIPLCNMYLDYIGCDRETFRRVSVYLDFIARRGAGETMTTAAWMRQFVTKHPEYKRDSVVSPSIAYDLMMECKRIGEGRLQCPEVMGNVQIDPIFPGEAYDVYLSGTKMGSSERAELVKAYRKRG-----PFHANKEGGKG 778
            LL+++    EAGLL+VG P+PW  ++PHL  VR  GV+QF++ Y +++ I  D L WG+E+EY I KVD   ++V +S R  +V++ L  +E ++  RSEGC W PE+GSWM+E TP  P  G  T L RVE NMRLRRKRLL +L  DEI+PT+  FPL GVGDF +PPA  GGP + S FVPDS INPHPRF  L KNIR RRG KVDI++PL+KD  TPEF      T      PRP I+MDAMAFGMGCCCLQVTFQARDI+ESRYM DQLAV++PI++AL+AA+PI +GRLADTD RWDTI+ +VDDRT     PAE    GEL    L      A  P       QR  KSRY SISTFIY+C                                              +   +++YNDI C VD+  +  L + G+D ALS+H+AHLF+RDPLVIF+  I EVDD    +HFEN+QSTNWQTVRWKPPP R +P+DPH+GWRTEFRS+EIQLTDFENAA+TVF++L+TRVIL  DL+LY+PLSKVD+NM +AH++D   K KF+FR ++                     ++ EG ++      S C +    G     E MTV +IL GKGDY  GLIPL + YL+YI  D  T + V +YLD + +R AGE MTTAAW+R+FVT HP Y +DS V+  IAYDL+  C+ IG G+  CPE++G+  I  +   EA+DV L  +KM    R  L+K + KR         A ++GG G
Sbjct:   17 LLLVAAGRGEAGLLRVGTPMPWGQAMPHLGYVRDHGVKQFVSRYRKVEGIENDELKWGDELEYAILKVDHVNKRVRVSTRAADVVKELQTREDEHAYRSEGCQWMPEYGSWMLEGTPKVPLRGLTTHLARVERNMRLRRKRLLATLADDEISPTMTNFPLFGVGDFTNPPAEAGGPTAQSKFVPDSVINPHPRFAALTKNIRERRGRKVDIQIPLYKDERTPEFLVEPPPTEGTEPPPRPTIHMDAMAFGMGCCCLQVTFQARDIEESRYMFDQLAVVSPIMLALSAATPIHRGRLADTDVRWDTISASVDDRTEEEMRPAEEPLSGELDVGGLMKDDENAPRP-------QRQPKSRYDSISTFIYNC-----------------------------------------RTGTDRCRVVNKYNDIDCPVDEPSRKALRKEGIDSALSKHIAHLFSRDPLVIFEGRIEEVDDDTEVDHFENLQSTNWQTVRWKPPPPRLSPNDPHVGWRTEFRSLEIQLTDFENAAYTVFIVLVTRVILALDLNLYIPLSKVDENMRRAHEKDAATKGKFFFRSNI------------------DYAQSPEGGEEGEIY--SPCDNDPQSG----LEEMTVLQILEGKGDY-PGLIPLVHAYLEYINADSSTIKDVELYLDHLRKRAAGEIMTTAAWLRKFVTSHPGYGKDSAVTDEIAYDLVQACQEIGLGKRACPELLGDRTIREVTRDEAWDVVLDSSKMEVQYRNRLIKKFVKRAAEKQRARDAQQQGGVG 728          
BLAST of NO25G01780 vs. NCBI_GenBank
Match: XP_008609334.1 (hypothetical protein SDRG_05398 [Saprolegnia diclina VS20] >EQC37172.1 hypothetical protein SDRG_05398 [Saprolegnia diclina VS20])

HSP 1 Score: 698.4 bits (1801), Expect = 2.700e-197
Identity = 382/807 (47.34%), Postives = 491/807 (60.84%), Query Frame = 0
Query:   23 GLLKVGRPLPWHVSLPHLRPVRRAGVRQFLNIYHRLKDIRQDGLLWGEEVEYGIFKVDRSKRQVWLSLRGKEVMESLIKKEQDY---TQRSEGCSWHPEFGSWMVESTPSRPYAGYATDLLRVESNMRLRRKRLLTSLDKDEIAPTVVAFPLMGVGDFVDPPAPPGGPVSDSAFVPDSCINPHPRFGTLVKNIRTRRGSKVDIRVPLFKDLDTPEF----KAW-------------------------------------------DEITRRVGA------PRP-YINMDAMAFGMGCCCLQVTFQARDIDESRYMHDQLAVLAPILMALTAASPIFKGRLADTDARWDTIAGAVDDRTPAERGELKGEALTAAANPLMAGEGIQRLFKSRYGSISTFIYSCGDEEQQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTITTEKEKRKTSAIDRYNDIPCEVDKELQAMLLEGGVDPALSRHVAHLFARDPLVIFDDAIEVDDSKTTEHFENIQSTNWQTVRWKPPPQRTT--PSDPHIGWRTEFRSMEIQLTDFENAAFTVFVILLTRVILTFDLSLYLPLSKVDDNMAKAHQRDGLLKEKFWFRKHLAPXXXXXXXXXXXXXXXXXXDKAFEGAQKAG--FVASSSCQDHVWQ--GTADEYELMTVEEILMGKGDYFLGLIPLCNMYLDYIGCDRETFRRVSVYLDFIARRGAGETMTTAAWMRQFVTKHPEYKRDSVVSPSIAYDLMMECKRIGEGRLQCPEVMGNVQIDPIFPGEAYDVYLSGTKMGSSERAELVKAYRKRG 767
            GLL VG PLPW  +L  L+ VRR G+ QFL  YHR+KDIR D L WG+E+EYGIF++D  ++ V LSLRG E++  L+ KE+ +       E C+W PE+G+WMVE+TP  PY G+ +DL RVE +MRLRR RLL+ L  +EIAPTV AFPL+GVGD++ PP    G +++S ++ D+ INPHPRFGTL  NIRTRR   VDIRVP+FKD+ T +      AW                                             +TRR  +      P P +I+MDAMAFGMG CCLQVTFQA++I ESR+++D L VL+PI++ALTAA+PI KGRLADTD RW TIA +VDDRTP ERG    E   A+    MAG+G++RL KSRY  IS FI +  D E                                            + +D YND+    D E    LL  GVD  L++H+AHLF RDPLV+F+  + +D+   T+HFENIQSTNWQTVRWKPP        S PHIGWRTEFRSMEIQLTDFENAAF+VF+ L++RVIL FDL+LY+PLSKV+ NM  AH+ +   +E F+FR+HLAP                      +   KAG     S++C D   +    A+ +E MT+ EI++GKG Y+ GLIPL N YLD+I CD  T R +  YL  IA+R  GE  T A WMR+FV  HP+Y  DSVVSPSIA+DLM  C +IGEG LQCPE++G+V+++PI    AY V L    + +++R  L++ Y +RG
Sbjct:    2 GLLVVGTPLPWDDALQWLKHVRRNGILQFLETYHRVKDIRGDVLKWGDELEYGIFELDHDEKTVRLSLRGSEILRELMDKEERFHRADHAEEECNWVPEYGAWMVEATPRVPYGGFTSDLRRVEQSMRLRRARLLSVLKPNEIAPTVTAFPLLGVGDYITPPVVKRGKIANSLYISDTIINPHPRFGTLTANIRTRRTENVDIRVPIFKDVYTKQMLEDEAAWVAEHARPTYGDATSGIKSPLKEGSRSPASATDIEVAIAPEPVFKRLTRRQSSFSEQCEPHPGFIHMDAMAFGMGMCCLQVTFQAKNIGESRHLYDHLGVLSPIMLALTAATPILKGRLADTDVRWATIAASVDDRTPQERGVAPQETAPASYTQ-MAGDGVKRLPKSRYEGISAFICNHKDGEDCH-----------------------------------------ANLDHYNDVEIPFDAESYETLLANGVDDVLAKHIAHLFIRDPLVLFEQRLHLDNKTATDHFENIQSTNWQTVRWKPPXXXAEDGASSPHIGWRTEFRSMEIQLTDFENAAFSVFMALVSRVILAFDLNLYVPLSKVNANMEIAHRVNAATEESFFFRRHLAPPD-------------------VDECHKAGCHIQCSATCVDDAAKCIHGAESFETMTIAEIMLGKGCYYPGLIPLVNAYLDHINCDDTTRRLLDKYLALIAKRATGELPTAATWMRRFVCSHPDYHHDSVVSPSIAHDLMDVCAKIGEGALQCPELLGDVRVEPIRGNTAYQVPLKSESVDTTKRCALLRRYVQRG 747          
BLAST of NO25G01780 vs. NCBI_GenBank
Match: OQR93434.1 (glutamate-cysteine ligase catalytic subunit [Achlya hypogyna])

HSP 1 Score: 695.7 bits (1794), Expect = 1.700e-196
Identity = 379/798 (47.49%), Postives = 494/798 (61.90%), Query Frame = 0
Query:   23 GLLKVGRPLPWHVSLPHLRPVRRAGVRQFLNIYHRLKDIRQDGLLWGEEVEYGIFKVDRSKRQVWLSLRGKEVMESLIKKEQDY---TQRSEGCSWHPEFGSWMVESTPSRPYAGYATDLLRVESNMRLRRKRLLTSLDKDEIAPTVVAFPLMGVGDFVDPPAPPGGPVSDSAFVPDSCINPHPRFGTLVKNIRTRRGSKVDIRVPLFKDLDTPEF----KAW------------------------------------DEITRRVGA------PRP-YINMDAMAFGMGCCCLQVTFQARDIDESRYMHDQLAVLAPILMALTAASPIFKGRLADTDARWDTIAGAVDDRTPAERGELKGEALTAAANPLMAGEGIQRLFKSRYGSISTFIYSCGDEEQQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTITTEKEKRKTSAIDRYNDIPCEVDKELQAMLLEGGVDPALSRHVAHLFARDPLVIFDDAIEVDDSKTTEHFENIQSTNWQTVRWKPPPQRT--TPSDPHIGWRTEFRSMEIQLTDFENAAFTVFVILLTRVILTFDLSLYLPLSKVDDNMAKAHQRDGLLKEKFWFRKHLAPXXXXXXXXXXXXXXXXXXDKAFEGAQKAGFVASSSCQDHVWQ--GTADEYELMTVEEILMGKGDYFLGLIPLCNMYLDYIGCDRETFRRVSVYLDFIARRGAGETMTTAAWMRQFVTKHPEYKRDSVVSPSIAYDLMMECKRIGEGRLQCPEVMGNVQIDPIFPGEAYDVYLSGTKMGSSERAELVKAYRKRG 767
            GLL VG PL W  +LP L  VRR G++QFL  YHR+KDIR D L WG+E+EYGIF++D   +   LSLRG EV+  L+ KE+ +       E C+W PE+G+WMVE+TP  PY G+ +DL RVE +MRLRR RLL+ L  +EIAPTV AFPL+GVGD+V PP    GP+++S ++ D  INPHPRFGTL  NIRTRR   VDIRVP+FKD+ T +      AW                                      +TRR  +      P P +I+MDAMAFGMG CCLQVTFQA++I ESR+++D L VL+PI++ALTAA+PI KGRLADTD RW TIA +VDDRTP ERG    ++  A+ +  MAG+G++RL KSRY  IS FI +  D E                                            + +D YN++    D+E    L   GVD  L++H+AHLF RDPLV+F+  + +D+   T+HFENIQSTNWQTVRWKPPP  +   P+ PHIGWRTEFRSMEIQLTDFENAAF+VF+ L++RVIL FDL+LY+PLSKV+ NM  AH+ +   +E F+FR+HLAP                  D+            S++C D   +    A+ +E MT+ EI++GKG Y+ GLIPL N YLD+I CD  T R +  YL  IA+R  GE  T AAWMR+FV  HP+Y  DSVV+P+IA+DL+  C +IGEG L CPE++G+V+I+PI    AY V L    + +++R+EL++ Y +RG
Sbjct:    2 GLLVVGTPLRWEDALPWLHHVRRNGIQQFLETYHRVKDIRGDVLKWGDELEYGIFELDPETKTARLSLRGSEVLHELMDKEERFHRADHAEEECNWVPEYGAWMVEATPRVPYGGFTSDLRRVEQSMRLRRARLLSVLKPNEIAPTVTAFPLLGVGDYVAPPVNRVGPIANSLYIDDRIINPHPRFGTLTANIRTRRTENVDIRVPIFKDVHTKQMLEDEAAWVAEHARPTYGDATSAIKSPQRGNSPKKEVAPSSEPLQRLTRRHPSFAESCEPHPGFIHMDAMAFGMGMCCLQVTFQAKNIGESRHLYDHLGVLSPIMLALTAATPILKGRLADTDVRWATIAASVDDRTPQERGVASADSAPASYSQ-MAGDGVKRLPKSRYEGISAFICNHKDGEDCH-----------------------------------------ANLDHYNNVEIPYDEESYETLRANGVDDILAKHIAHLFIRDPLVLFEQRLHLDNKTATDHFENIQSTNWQTVRWKPPPPPSGDGPAPPHIGWRTEFRSMEIQLTDFENAAFSVFMALVSRVILAFDLNLYVPLSKVNANMEIAHRVNAATEEVFYFRRHLAP---------------PDVDECHRA--DCHVQCSATCLDDAAKCIHGAESFETMTIAEIMLGKGCYYPGLIPLVNAYLDHIQCDDTTRRLLDKYLALIAKRATGELPTAAAWMRRFVRAHPDYAHDSVVTPAIAHDLLDTCAKIGEGTLPCPELLGDVRIEPIRGNTAYQVPLKSESVDTAKRSELLQRYVQRG 740          
BLAST of NO25G01780 vs. NCBI_GenBank
Match: CBN79467.1 (Glutamate cysteine ligase [Ectocarpus siliculosus])

HSP 1 Score: 694.9 bits (1792), Expect = 3.000e-196
Identity = 378/748 (50.53%), Postives = 467/748 (62.43%), Query Frame = 0
Query:   71 EVEYGIFKVDRSKRQVWLSLRGKEVMESLIKKEQDYTQRSEGCSWHPEFGSWMVESTPSRPYAGYATDLLRVESNMRLRRKRLLTSLDKDEIAPTVVAFPLMGVGDFVDPPAPP----------------GGPVSDSAFVPDSCINPHPRFGTLVKNIRTRRGSKVDIRVPLFKDLDTPEFKAWD---------------EITRRVG---APRPYINMDAMAFGMGCCCLQVTFQARDIDESRYMHDQLAVLAPILMALTAASPIFKGRLADTDARWDTIAGAVDDRTPAERGELKGE--ALTAAANPLMAGEGIQRLFKSRYGSISTFIYSCGDEEQQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTITTEKEKRKTSAIDRYNDIPCEVDKELQAMLLEGGVDPALSRHVAHLFARDPLVIFDDAI-EVDDSKTTEHFENIQSTNWQTVRWKPPPQRTTPSDPHIGWRTEFRSMEIQLTDFENAAFTVFVILLTRVILTFDLSLYLPLSKVDDNMAKAHQRDGLLKEKFWFRKHLAPXXXXXXXXXXXXXXXXXXDKAFEGAQKAGFVASSSCQD-HVWQG---------------TADEYELMTVEEILMGKGDYFLGLIPLCNMYLDYIGCDRETFRRVSVYLDFIARRGAGETMTTAAWMRQFVTKHPEYKRDSVVSPSIAYDLMMECKRIGEGRLQCPEVMGNVQIDPIFPGEAYDVYLSGTKMGSSERAELVKAYRKR 766
            +VEYGIFKVD   + V LSLRG E+++ L +KE++   R EGC+WHPE+GSWMVE+TP  P++GYA DL+RVE NMRLRRKR+L+ L   E+APT+  FPLMGVGDFV   A P                 GPV++SA++PD  INPHPRFG L KNIR RRG+ VDIR+PLF+D +TPEF   +                I    G   A  P+++MDAMAFGMGCCCLQVTFQARD+DESRYM+DQLAVL+P+++ALTAA+PI KGRL DTD RWDTIA +VDDRTPAERG L  +  A  AA +P M G+G+ RL+KSRY SIS++++ C                                          +E+     ++ YNDIPCEVDK  ++ L+E GVD AL+ HVAHLFARDPLVIFD A+ EVDD+  TEHFENIQSTNWQTVRWKPPP R     P IGWRTEFRSME+Q TD ENAAFTVFV+L+TRVIL FDL+LY+PLS                  KFW+R H+AP                XX          G   S  C++ H   G                A  +E MT+EEI++GKGDY+ GL+PL   YL +IGCD  T   V  Y+  I +R +GE  T+A+W+R FV +HP YK+DSVVS  IA+DL+ME + IGEG    P ++G + I PI P +AYDV LS TK+  S  A LVK Y  R
Sbjct:   22 KVEYGIFKVDPEAKTVKLSLRGAEILKELDEKEKNSLHRLEGCTWHPEYGSWMVEATPRGPFSGYAADLVRVERNMRLRRKRILSVLRPGEVAPTMTCFPLMGVGDFVASSAVPPSASDSSERPSSRWHVRGPVANSAYIPDVVINPHPRFGALTKNIRARRGNNVDIRIPLFRDENTPEFHGGEAXXXXXXXXXXXXXCPINPTQGLEKAHGPHVHMDAMAFGMGCCCLQVTFQARDVDESRYMYDQLAVLSPVMLALTAATPILKGRLVDTDVRWDTIATSVDDRTPAERGLLDADPAAKEAARDPSMVGDGVARLYKSRYDSISSYLHYC----------------------------------------KRREENPMHVLETYNDIPCEVDKGAESFLVENGVDRALAFHVAHLFARDPLVIFDGAVEEVDDTTATEHFENIQSTNWQTVRWKPPPPRENEDSPRIGWRTEFRSMEMQTTDAENAAFTVFVVLVTRVILAFDLNLYIPLS------------------KFWWRMHMAPPDNAKAAAANHSGAGEXXXXXXXXXXXXGAPMSHPCEESHGGDGGTSSAVDQEHKACDVHAQGHEEMTIEEIMLGKGDYYPGLVPLVYAYLGHIGCDAATMDTVDQYMILICKRASGELKTSASWIRDFVHEHPAYKQDSVVSEEIAHDLVMELRAIGEGEKHAPSLLGGLHISPIRPKQAYDVPLSSTKVSGSCLAFLVKKYTMR 711          
BLAST of NO25G01780 vs. NCBI_GenBank
Match: XP_012197537.1 (hypothetical protein SPRG_03567 [Saprolegnia parasitica CBS 223.65] >KDO31647.1 hypothetical protein SPRG_03567 [Saprolegnia parasitica CBS 223.65])

HSP 1 Score: 694.1 bits (1790), Expect = 5.100e-196
Identity = 380/807 (47.09%), Postives = 491/807 (60.84%), Query Frame = 0
Query:   23 GLLKVGRPLPWHVSLPHLRPVRRAGVRQFLNIYHRLKDIRQDGLLWGEEVEYGIFKVDRSKRQVWLSLRGKEVMESLIKKEQDY---TQRSEGCSWHPEFGSWMVESTPSRPYAGYATDLLRVESNMRLRRKRLLTSLDKDEIAPTVVAFPLMGVGDFVDPPAPPGGPVSDSAFVPDSCINPHPRFGTLVKNIRTRRGSKVDIRVPLFKDLDTPEF----KAW-------------------------------------------DEITRRVGA------PRP-YINMDAMAFGMGCCCLQVTFQARDIDESRYMHDQLAVLAPILMALTAASPIFKGRLADTDARWDTIAGAVDDRTPAERGELKGEALTAAANPLMAGEGIQRLFKSRYGSISTFIYSCGDEEQQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTITTEKEKRKTSAIDRYNDIPCEVDKELQAMLLEGGVDPALSRHVAHLFARDPLVIFDDAIEVDDSKTTEHFENIQSTNWQTVRWKPPPQRTT--PSDPHIGWRTEFRSMEIQLTDFENAAFTVFVILLTRVILTFDLSLYLPLSKVDDNMAKAHQRDGLLKEKFWFRKHLAPXXXXXXXXXXXXXXXXXXDKAFEGAQKAG--FVASSSCQDHVWQ--GTADEYELMTVEEILMGKGDYFLGLIPLCNMYLDYIGCDRETFRRVSVYLDFIARRGAGETMTTAAWMRQFVTKHPEYKRDSVVSPSIAYDLMMECKRIGEGRLQCPEVMGNVQIDPIFPGEAYDVYLSGTKMGSSERAELVKAYRKRG 767
            GLL VG PLPW  +L  L+ VRR G+ QFL  YHR+KDIR D L WG+E+EYGIF++D  ++ V LSLRG E++  L+ KE+ +       E C+W PE+G+WMVE+TP  PY G+ +DL RVE +MRLRR RLL+ L  +EIAPTV AFPL+GVGD++ PP    GP+++S ++ D+ INPHPRFGTL  NIR RR   VDIRVP+FKD+ T +      AW                                             +TRR  +      P P +I+MDAMAFGMG CCLQVTFQA++I ESR+++D L VL+PI++ALTAA+PI KGRLADTD RW TIA +VDDRTP ERG    E    +    MAG+G++RL KSRY  IS FI +  D +                                            + +D YND+    D E  A LL  GVD  L++H+AHLF RDPLV+F+  + +D+   T+HFENIQSTNWQTVRWKP         + PHIGWRTEFRSMEIQLTDFENAAF+VF+ L++RVIL FDL+LY+PLSKV+ NM  AH+ +   +E F+FR+HLAP                      +   KAG     S++C D   +    A+ +E MT+ EI++GKG Y+ GLIPL N YLD+I CD  T R +  YL  IA+R  GE  T A WMR+FV  HP+Y  DSVVSPSIA+DLM  C +IGEG LQCPE++G+V+++PI    AY V L    + +++R EL++ Y +RG
Sbjct:    2 GLLVVGTPLPWDDALQWLKHVRRNGILQFLETYHRVKDIRGDVLKWGDELEYGIFELDHDEKTVRLSLRGSEILRELMDKEERFHRADHAEEECNWVPEYGAWMVEATPRVPYGGFTSDLRRVEQSMRLRRARLLSVLKPNEIAPTVTAFPLLGVGDYITPPVVKRGPIANSLYISDTIINPHPRFGTLTANIRKRRTENVDIRVPIFKDVFTKQMLDDEAAWVAEHARPTYGDATSGIKSPLKEGSRSPTSAKDIEVAVAPEPVFARLTRRQSSFSEQCEPYPGFIHMDAMAFGMGMCCLQVTFQAKNIGESRHLYDHLGVLSPIMLALTAATPILKGRLADTDVRWATIAASVDDRTPQERGAAPLETAPPSYKQ-MAGDGVKRLPKSRYEGISAFICNHKDGDDCH-----------------------------------------ANLDHYNDVEIPFDAESYATLLSNGVDDVLAKHIAHLFIRDPLVLFEQRLHLDNKTATDHFENIQSTNWQTVRWKPXXXXXXDGAASPHIGWRTEFRSMEIQLTDFENAAFSVFMALVSRVILAFDLNLYVPLSKVNANMEIAHRVNAATEESFFFRRHLAPPD-------------------VDECHKAGCHVQCSATCVDDAAKCIHGAESFETMTIAEIMLGKGCYYPGLIPLVNAYLDHINCDDTTRRLLDKYLALIAKRATGELPTAATWMRRFVCSHPDYHHDSVVSPSIAHDLMDVCAKIGEGALQCPELLGDVRVEPIRGNTAYQVPLKSESVDTTKRCELLRRYVQRG 747          
The following BLAST results are available for this feature:
BLAST of NO25G01780 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes)
Total hits: 20
Match NameE-valueIdentityDescription
XP_002297305.12.300e-20951.13glutamate-cysteine ligase, partial [Thalassiosira ... [more]
XP_002179736.12.900e-20750.13predicted protein [Phaeodactylum tricornutum CCAP ... [more]
OEU14148.18.300e-20750.52gamma glutamylcysteine synthetase [Fragilariopsis ... [more]
OEU14149.18.300e-20750.52gamma glutamylcysteine synthetase [Fragilariopsis ... [more]
XP_002291923.17.100e-20652.75glutamate-cysteine ligase, partial [Thalassiosira ... [more]
CBJ30287.12.300e-20449.94Glutamate cysteine ligase [Ectocarpus siliculosus][more]
XP_008609334.12.700e-19747.34hypothetical protein SDRG_05398 [Saprolegnia dicli... [more]
OQR93434.11.700e-19647.49glutamate-cysteine ligase catalytic subunit [Achly... [more]
CBN79467.13.000e-19650.53Glutamate cysteine ligase [Ectocarpus siliculosus][more]
XP_012197537.15.100e-19647.09hypothetical protein SPRG_03567 [Saprolegnia paras... [more]

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Relationships

This gene is member of the following syntenic_region feature(s):

Feature NameUnique NameSpeciesType
ncniR045ncniR045Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ngnoR073ngnoR073Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region


The following polypeptide feature(s) derives from this gene:

Feature NameUnique NameSpeciesType
NO25G01780.1NO25G01780.1-proteinNannochloropsis oceanica (N. oceanica IMET1)polypeptide


The following gene feature(s) are orthologous to this gene:

Feature NameUnique NameSpeciesType
jgi.p|Nanoce1779_2|604026gene_8788Nannochloropsis oceanica (N. oceanica CCMP1779)gene


The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
NO25G01780.1NO25G01780.1Nannochloropsis oceanica (N. oceanica IMET1)mRNA


Sequences
The following sequences are available for this feature:

gene sequence

>NO25G01780 ID=NO25G01780|Name=NO25G01780|organism=Nannochloropsis oceanica|type=gene|length=5538bp
ATGTCCGTTTGCGTCCTTCTCATAGGCCTTGTGCTCCTCATTCTATCGCC
CTCCCCCACCGAGGCCGGCCTGCTCAAGgtacgcacgcaccaacagattc
acacgcatacaccctcagatacacatatacgttcacacatgttttctagc
ctgcctgcttctcacgtctttcaacggctcctactctaccatgtccttta
agccttatcgtccctccttccctccctcattccttcccccctccccaagG
TCGGCCGCCCCCTCCCCTGGCACGTCTCCTTACCACATCTCCGTCCCGTT
CGGCGCGCAGGTGTACGTCAATTTCTAAATATTTACCACCGCCTCAAGGA
CATTAGGCAGGATGGTCTTTTGTGGGGGGAGGAGGTGGAGTACGGCATAT
TCAAGgtaagaagggagggagggagggagggaaggagggagccagggcac
ggcaagcgcgactcgttacatgccgtattggtaattcctatctcaacaac
agGTGGATCGAAGCAAGAGACAGGTTTGGCTGTCCCTCCGTGGGAAGGAA
GTCATGGAGAGCCTTATAAAGAAGGAGCAAGACTACACCCAACGgtgcgc
ccccctcccttcctcccttcctccctccctccctcactcactctattact
catccatccatctttccctccttttctctgtcgttcagATCCGAAGGCTG
CAGCTGGCACCCGGAGTTTGGATCCTGGATGGTCGAGTCCACGCCCTCAA
GGCCCTATGCAGGgtgagggagggagggagggagggagggagggaaggga
ccataccaactatgccttccagggccttccagacaggccgtatcagcatg
agggaggcaaggtgtctaagcatggaaggggggagggtgggaagagaagg
agatgacatgatcaaagccaaaggagtctctcacgcgtcgcccctccctc
ccttccttcctccctccctccctcccttcctccctccctttcccctgtag
CTATGCGACAGACCTGTTGCGCGTCGAGAGTAACATGCGCCTTCGGCGGA
AGCGTCTTCTGACCTCCCTGGACAAGGACGAGATCGCCCCTACGGTCGTG
GCATTTCCCCTCATGGGCGTGGGGGATTTTGTGGACCCCCCCGCACCACC
AGGGgtaaggagaaggagattccagcacgttttccctcctcctcctcctc
ctcttcctcctcctcctcctctgtctctctgcatctgtttctgtctccgt
taccgtaacatggcctcgttttttctccctcccttccctcccctggccaa
ccaaccgttcttcccctccctccctccctccctccctccctccctccctc
cctccctcccttcctccctccctcagGGCCCCGTCTCGGACTCGGCCTTC
GTCCCCGACAGCTGCATTAATCCTCACCCTCGTTTCGGCACGCTGGTGAA
GAACATTCGCACCCGCCGAGGGAGTAAGGTCGACATCCGCGTTCCCCTCT
TCAAGGACCTGGACACTCCTGAGTTCAAGGCTTGGGACGgtacgtgccct
ccctccctccctccctccctccctccctccctcccccccatatatggtgc
atgtctatgtggatgtgtgtgtctggatttcgtcatggatcatccctccc
tccctccctccctccctcccttcctccctcccttcctccctccctccctt
cctccctccctcccttccttcttccctttcgatgacataccacccggaga
cgttttgcactttaccctccctctcacctacacctccctccctccctccc
tctcacctacacctccctctctccctccctcctctccagAGATCACCAGG
AGGGTAGGCGCCCCTCGGCCGTACATCAATATGGACGCCATGGCCTTCGG
CATGGGCTGCTGCTGCTTGCAGgtaggccctccttccctccctccctccc
tctttccctccctccctccctccctccctccctctttccctccctccctc
cctccctccctccctccctctctctccctgcctctatcacccgtggtgcc
ttttgttcatccccccatccgcacaccccatctcccctccttcattccct
tgctccctccctccctccctccctccctccatccctccctgccctagGTC
ACCTTCCAAGCGCGCGACATTGACGAATCCCGGTACATGCACGACCAGCT
CGCTGTCCTTGCCCCAATCCTCATGGCACTCACGGCTGCCAGCCCCATCT
TCAAGgtaagccagccctccctccctccctccctccctccctccctccct
ccctccctccctccctccctccctccctccctcctccacataacccacat
gtgggctatatacatatatatgtccacataacccacatgttggttatata
tatatatatatatatatatatatatatatatatgtatgtgtatccacata
agccacatgaaagctatatacatatatatatccacataacccacatgttg
gttatgtatacatatatgtatgtgtatccacataagccacatgaaagcta
tatacatatatatatatccacataacccccatgtgggatatgctccattg
gcccaggcgccgagccatcttctcctttatcttactccctccctccctcc
ctccctccctccctccctccctccctccctccctccctccttcccagGGT
CGCCTGGCGGATACGGATGCTCGATGGGACACCATCGCTGGCGCTGTCGA
TGATCGCACGCCGGCTGAACGGGGAGAGTTAAAGGGGGAGgtgaggaacg
aagggagggagggagggtgggagggagggagggagggagggaagaggcca
ggtagacatgtggctctcgatctgtcaactcacccctcctgttcctccct
cctcctaccttccctccctctctccctcctccttccctcccaatctcttc
ctcctccttccatccctccctctctccctcctccttccctccctccctct
ctccctcctccctccctccctccgtctctctctagGCCTTGACGGCAGCG
GCTAACCCCCTCATGGCCGGTGAGGGCATTCAGCGTCTCTTTAAGTCACG
CTATGGCTCTATATCCACGTTTATTTACAGCTGTGGGGATGAGGAGCAGC
AGAGTCAGGAGGAGGAAGAGGAGGAGGAGGGAGGGAAGGAGGGAGGGAAG
GAGGGAAGGAAGGAGGGAAGGAAGGAGGGAGGGAAGGTCAAGGCGACGAT
CACGACTGAAAAGGAAAAAAGGAAGACGAGTGCCATTGACCGTTACAATG
ACATCCCCTGTGAGGTGGACAAGGAGTTACAGgtaggggagtaggaaagg
agggagggagggacgaacgcacggtttcaccatacgtttcatgctcacat
ccttctccccctccctccttccctccctcccccagGCCATGCTTCTAGAA
GGTGGAGTGGACCCGGCGCTCTCCCGGCACGTGGCCCATCTCTTCGCCCG
CGATCCTTTGGTCATCTTCGACGATGCAATCGAGGTGGATGACAGCAAGA
CCACCGAGCACTTTGAGAATATACAGgtgggcactacccttccctccctc
ctctccctccctccctccctccctctcccatctcgtctcggtcgatgttc
tacaagggctcccatccatctatataagacactaacacctccctccctcc
ctccctccctccctccctccctccctccctccttccctcgcttcccagTC
CACAAACTGGCAAACCGTTCGTTGGAAGCCCCCACCTCAACGGACTACGC
CCAGCGACCCGCACATTGGCTGGAGGACGGAGTTCAGgtgcggagggcgg
gggggagggcgggggggaggccgggaggtagggagggcaagggtacgatt
tggtctctgttgaaaaaggtggtatggctatgctgggtgtttctcgtgcc
cacgcgaagaccctccctccctcccttcctcccatttctagGTCCATGGA
GATCCAGCTCACTGATTTCGAGAACGCCGCCTTCACTGTCTTCGTCATCC
TCCTGACCCGCGTCATTTTGACGTTCGATCTTTCGCTCTACCTGCCCTTG
AGCAAGgtagggaatcaggaagggagggagggagggagggaggaagggat
ggagggaggacagggaggagcatggcaaggaatgccttgtccacacgatg
aaaatctgaccacccttccctccctccctccctccctccctccctccctc
tttccagGTGGACGACAATATGGCCAAGGCCCACCAACGCGACGGTCTGC
TCAAGGAGAAATTCTGGTTCCGAAAACACCTCGCCCCTCCCCTGGCCTCC
TCCTCCTCCTCCTCCTCCTCCTCCTCCTCGTCGGAGAAGAGGGACAAGGC
GTTTGAAGGCGCTCAGAAGGCAGGCTTTGTCGCCTCCTCCTCGTGCCAGG
ACCACGTCTGGCAAGGGACAGCTGACGAGTACGAGCTCATGACGGTGGAG
GAGATATTGATGGGGAAAGGCGATTACTTCCTAGGCCTCATCCCGCTGTG
TAATATGTATTTGGATTACATCGGgtatgtggcccccgtgtccctccttc
ccccgctccctccctccctccctccctcccaccgtccctccctcctcact
ccttccctccctctcctcttccctcccctcacttgtcctcttccccgtcg
actcacaaccgccccctccctccctcccgcccgccctccctccctcccgc
cctccctctctccccagCTGCGACCGCGAAACCTTCCGGCGTGTCTCGGT
CTACTTGGACTTTATTGCCCGTCGGGGTGCGGGCGAGACAATGACAACCG
CGGCGTGGATGCGGCAATTCGTCACCAAGCACCCGGAATACAAAgtaggg
aggcagggagggaggggcttatacaggggcgtggcctgttgcgtattgtt
tgcatccgatgaaatctgtgggatgtattcatttccttctctcttccttc
cttccctcccccctccctcccacccaccctccctccctccttgcagCGGG
ACTCGGTCGTGTCCCCCTCGATCGCCTATGACCTGATGATGGAGTGCAAG
AGGATAGGAGAAGGGCGCCTTCAGgtagggaggaaggaagaagggaggga
ggttgggagggagggagggaggtagggagtgaacaaaaagatggataggt
agaggtcgacgggctgtcgcacaacacccctcatattcacccttcccccc
tccttccctccctccctccttcacccgcagTGTCCCGAGGTCATGGGCAA
CGTGCAGATCGATCCGATCTTCCCCGGGGAGGCCTATGACGTGTATTTGT
CGGGCACCAAGATGGGGAGCAGCGAGCGGGCCGAGCTCGTGAAGGCGTAC
CGCAAGAGAGGGCCCTTCCATGCCAACAAGGAGGGAGGGAAGGGGAGAAA
GGGAGGGAAGGAGGGGGAGACGAAGGGGAGAGAGACGGAAGGTGGTAGGG
AGGAGAAGAATGATGAGTTTTTCCATTACCGGCAGTGA
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protein sequence of NO25G01780.1

>NO25G01780.1-protein ID=NO25G01780.1-protein|Name=NO25G01780.1|organism=Nannochloropsis oceanica|type=polypeptide|length=808bp
MSVCVLLIGLVLLILSPSPTEAGLLKVGRPLPWHVSLPHLRPVRRAGVRQ
FLNIYHRLKDIRQDGLLWGEEVEYGIFKVDRSKRQVWLSLRGKEVMESLI
KKEQDYTQRSEGCSWHPEFGSWMVESTPSRPYAGYATDLLRVESNMRLRR
KRLLTSLDKDEIAPTVVAFPLMGVGDFVDPPAPPGGPVSDSAFVPDSCIN
PHPRFGTLVKNIRTRRGSKVDIRVPLFKDLDTPEFKAWDEITRRVGAPRP
YINMDAMAFGMGCCCLQVTFQARDIDESRYMHDQLAVLAPILMALTAASP
IFKGRLADTDARWDTIAGAVDDRTPAERGELKGEALTAAANPLMAGEGIQ
RLFKSRYGSISTFIYSCGDEEQQSQEEEEEEEGGKEGGKEGRKEGRKEGG
KVKATITTEKEKRKTSAIDRYNDIPCEVDKELQAMLLEGGVDPALSRHVA
HLFARDPLVIFDDAIEVDDSKTTEHFENIQSTNWQTVRWKPPPQRTTPSD
PHIGWRTEFRSMEIQLTDFENAAFTVFVILLTRVILTFDLSLYLPLSKVD
DNMAKAHQRDGLLKEKFWFRKHLAPPLASSSSSSSSSSSSEKRDKAFEGA
QKAGFVASSSCQDHVWQGTADEYELMTVEEILMGKGDYFLGLIPLCNMYL
DYIGCDRETFRRVSVYLDFIARRGAGETMTTAAWMRQFVTKHPEYKRDSV
VSPSIAYDLMMECKRIGEGRLQCPEVMGNVQIDPIFPGEAYDVYLSGTKM
GSSERAELVKAYRKRGPFHANKEGGKGRKGGKEGETKGRETEGGREEKND
EFFHYRQ*
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