NO20G02130, NO20G02130 (gene) Nannochloropsis oceanica

Overview
NameNO20G02130
Unique NameNO20G02130
Typegene
OrganismNannochloropsis oceanica (N. oceanica IMET1)
Sequence length1701
Alignment locationchr20:637000..638714 +

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Properties
Property NameValue
DescriptionHeat shock protein hsp20
Mutants
Expression

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
chr20genomechr20:637000..638714 +
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
PRJNA7699582024-08-13
GSE1786722023-12-15
PRJNA9336932023-10-26
PRJNA9434492023-07-19
PXD0160542021-01-14
PXD0166992021-01-08
GSE1499042020-10-10
NoIMET1_WT_HS2020-09-29
PXD0100302020-03-16
GSE1396152020-03-11
PRJNA2413822020-03-11
BLASTP analysis of N. oceanica IMET1 genes2019-07-11
InterPro analysis for N. oceanica IMET1 genes2019-07-11
PXD0087212019-04-30
PRJNA1821802019-04-25
Gene prediction for N. oceanica IMET12017-10-24
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR008978HSP20-like_chaperone
IPR002068A-crystallin/Hsp20_dom
Homology
BLAST of NO20G02130 vs. NCBI_GenBank
Match: EWM22022.1 (heat shock protein hsp20 [Nannochloropsis gaditana])

HSP 1 Score: 165.2 bits (417), Expect = 2.500e-37
Identity = 91/154 (59.09%), Postives = 105/154 (68.18%), Query Frame = 0
Query:    1 MALSRF---DPFGSMLTERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQAIVPNLSLDVKEKNNEFEVAVDAPGMKKEDFDVTFENNVLRVACERKEEKEEEDARWHISERRWGSCSRSVRLPRAAGCENIKAEYDAGVLKVKRMRGKKAE 152
            MALSR+   DP GS L ER                                Q +VP+L+LDVKEKNNEF+VA+DAPGMKKEDFDVTF+NNVLRVACERKEEKEEEDARWHISERRWGS SRSVRLPR A  + I AEYD+GVLK+   + +++E
Sbjct:    1 MALSRYWRDDPLGSNLIER---DPFADFFSTGGGWLTPSVGGALSTGTSRGQPVVPSLNLDVKEKNNEFQVAIDAPGMKKEDFDVTFDNNVLRVACERKEEKEEEDARWHISERRWGSSSRSVRLPRTANGDGITAEYDSGVLKIHVPKTQESE 151          
BLAST of NO20G02130 vs. NCBI_GenBank
Match: EWM28800.1 (heat shock protein hsp20 [Nannochloropsis gaditana])

HSP 1 Score: 90.1 bits (222), Expect = 1.000e-14
Identity = 43/113 (38.05%), Postives = 72/113 (63.72%), Query Frame = 0
Query:   53 PNLSLDVKEKNNEFEVAVDAPGMKKEDFDVTFENNVLRVACERKEEKEEEDARWHISERRWGSCSRSVRLPRAAGCENIKAEYDAGVLKVKRMRGKKAEREGXXXXXXQDEAF 166
            P++ +D+ E + EF + VD PG KKED D++ ++++L V  ER+EE  E+   +H+ ERR G   RS+RLPR+A  ++++A+Y  GVL++     KKAE+         D+++
Sbjct:   33 PSMQMDISETDREFRIQVDLPGHKKEDIDISVDDDILTVQTERREEHSEDTENFHVKERRVGKAKRSIRLPRSADTDSVRAQYADGVLRI--CVEKKAEKGQSKSIKITDQSY 143          
BLAST of NO20G02130 vs. NCBI_GenBank
Match: OGO72027.1 (heat-shock protein Hsp20 [Chloroflexi bacterium RBG_19FT_COMBO_56_12])

HSP 1 Score: 84.0 bits (206), Expect = 7.300e-13
Identity = 40/98 (40.82%), Postives = 63/98 (64.29%), Query Frame = 0
Query:   54 NLSLDVKEKNNEFEVAVDAPGMKKEDFDVTFENNVLRVACERKEEKEEEDARWHISERRWGSCSRSVRLPRAAGCENIKAEYDAGVLKVKRMRGKKAE 152
            +L+LDV E  +EF V    PG+  +D ++TF NN L +  E KEEK+ E A +H+ ERR+GS +RS+ LP     + I+A Y+ GVLK++  + ++ +
Sbjct:   39 DLALDVTESEDEFTVKASLPGINPDDLEITFTNNTLTIKGETKEEKDVEKAHYHLRERRYGSFARSITLPAGIETDKIQANYEVGVLKLRLPKAEEVK 136          
BLAST of NO20G02130 vs. NCBI_GenBank
Match: OQY88376.1 (hypothetical protein B6D39_11330 [Anaerolineae bacterium UTCFX2])

HSP 1 Score: 83.6 bits (205), Expect = 9.500e-13
Identity = 39/102 (38.24%), Postives = 69/102 (67.65%), Query Frame = 0
Query:   49 QAIVPNLSLDVKEKNNEFEVAVDAPGMKKEDFDVTFENNVLRVACERKEEKEEEDARWHISERRWGSCSRSVRLPRAAGCENIKAEYDAGVLKVKRMRGKKA 151
            Q +  NL+LDV E ++E+ V+   PG+  +D ++T+++NVL +  E +E++E ++ R+H+ ERR+GS SRS+ LP +   + IKA+Y  GVL +   + ++A
Sbjct:   36 QPMTWNLALDVAETDDEYRVSASLPGIDPDDLEITYDSNVLTIKGEMREDQEVKEERYHMRERRYGSFSRSISLPTSVKADKIKADYSKGVLTLHLPKSEEA 137          
BLAST of NO20G02130 vs. NCBI_GenBank
Match: PKL36239.1 (hypothetical protein CVV44_18670 [Spirochaetae bacterium HGW-Spirochaetae-1])

HSP 1 Score: 83.6 bits (205), Expect = 9.500e-13
Identity = 46/95 (48.42%), Postives = 61/95 (64.21%), Query Frame = 0
Query:   57 LDVKEKNNEFEVAVDAPGMKKEDFDVTFENNVLRVACERKEEKEEEDARWHISERRWGSCSRSVRLPRAAGCENIKAEYDAGVLKVKRMRGKKAE 152
            LDV+E      V  + PG+ ++D DVT ENNVL +A E+K EKEE+D R+ ISERR+GS SRS+ LP     + IKA +  GVL V   R + A+
Sbjct:   47 LDVQEDAKAITVKAEIPGIDEKDLDVTLENNVLTIAGEKKLEKEEKDKRYTISERRFGSFSRSISLPEGVSPDRIKAAFKNGVLSVTIPRDESAQ 141          
BLAST of NO20G02130 vs. NCBI_GenBank
Match: OGP94089.1 (molecular chaperone [Deltaproteobacteria bacterium RBG_16_47_11])

HSP 1 Score: 82.4 bits (202), Expect = 2.100e-12
Identity = 42/98 (42.86%), Postives = 62/98 (63.27%), Query Frame = 0
Query:   56 SLDVKEKNNEFEVAVDAPGMKKEDFDVTFENNVLRVACERKEEKEEEDARWHISERRWGSCSRSVRLPRAAGCENIKAEYDAGVLKVKRMRGKKAERE 154
            SLDV E  +   V  + PGM  +  D++F + +L V  E K+EKEE+D  +H+ ERR+GS SRSVRLP     + IKA Y  GVLK+   + ++A+++
Sbjct:   45 SLDVSETKDNIMVKAELPGMDPKKIDISFADGILTVKGENKQEKEEKDENYHMVERRYGSFSRSVRLPHEVKSDKIKANYKEGVLKITLPKSEEAKKK 142          
BLAST of NO20G02130 vs. NCBI_GenBank
Match: EPZ32380.1 (Alpha crystallin/Hsp20 domain-containing protein [Rozella allomycis CSF55])

HSP 1 Score: 81.6 bits (200), Expect = 3.600e-12
Identity = 41/102 (40.20%), Postives = 63/102 (61.76%), Query Frame = 0
Query:   51 IVPNLSLDVKEKNNEFEVAVDAPGMKKEDFDVTFENNVLRVACERKEEKEEEDARWHISERRWGSCSRSVRLPRAAGCENIKAEYDAGVLKVKRMRGKKAER 153
            I P L+LD+ E +N + +  D PG+ KED  V  E+NVL V  ER+++++E +   H++ER +G   RSVRLP     E  +A  D GVLK++  + + AE+
Sbjct:   40 IKPQLALDISELDNAYVIQADLPGIAKEDISVNLEDNVLTVETERRDQRDENNGTIHLTERFYGKMKRSVRLPAQVDPEKTEATLDNGVLKLRVAKSEAAEK 141          
BLAST of NO20G02130 vs. NCBI_GenBank
Match: WP_015897492.1 (Hsp20/alpha crystallin family protein [Acidobacterium capsulatum] >ACO34416.1 heat shock protein, Hsp20 family [Acidobacterium capsulatum ATCC 51196])

HSP 1 Score: 80.9 bits (198), Expect = 6.200e-12
Identity = 38/87 (43.68%), Postives = 57/87 (65.52%), Query Frame = 0
Query:   57 LDVKEKNNEFEVAVDAPGMKKEDFDVTFENNVLRVACERKEEKEEEDARWHISERRWGSCSRSVRLPRAAGCENIKAEYDAGVLKVK 144
            +D+ E  +   + ++ PGMK+EDFD+  ENN L +  ERK EKEE++  +H  ERR+G+  RS  +P     E +KA YDAGVL+++
Sbjct:   44 VDIYEDEHRIVLKLEVPGMKQEDFDIQLENNTLTIRGERKFEKEEKEENFHRIERRYGTFFRSFAVPNTVNTEQVKAGYDAGVLRIE 130          
BLAST of NO20G02130 vs. NCBI_GenBank
Match: KDD76497.1 (hypothetical protein H632_c205p1 [Helicosporidium sp. ATCC 50920])

HSP 1 Score: 80.5 bits (197), Expect = 8.000e-12
Identity = 44/95 (46.32%), Postives = 60/95 (63.16%), Query Frame = 0
Query:   50 AIVPNLSLDVKEKNNEFEVAVDAPGMKKEDFDVTFENNVLRVACERKEEKEE--EDARWHISERRWGSCSRSVRLPRAAGCENIKAEYDAGVLKV 143
            A+   +++DV E  + F+V  D PG+K++D  VT  ++VLR+  E  EEKEE  ED  WH  ER     SRS+RLPR A  E+IKA+Y  GVL +
Sbjct:   27 ALTRGMAVDVTESKDAFQVQADVPGVKRDDIHVTVHDDVLRLNVESTEEKEEQAEDKTWHRYERSGTWASRSIRLPRNADLEHIKAKYAEGVLSL 121          
BLAST of NO20G02130 vs. NCBI_GenBank
Match: WP_050058308.1 (Hsp20/alpha crystallin family protein [Silvibacterium bohemicum])

HSP 1 Score: 80.5 bits (197), Expect = 8.000e-12
Identity = 43/102 (42.16%), Postives = 65/102 (63.73%), Query Frame = 0
Query:   50 AIVPNLSLDVKEKNNEFEVAVDAPGMKKEDFDVTFENNVLRVACERKEEKEEEDARWHISERRWGSCSRSVRLPRAAGCENIKAEYDAGVLKVKRMRGKKAE 152
            A VP   +D+ E  ++  + ++ PG+K+EDFD+  ENN L V  ERK EKEE++  +H  ERR+GS  RS  +P     E +KA YDAGVL+++  +  +A+
Sbjct:   40 AFVP--PVDIYEDQHKIVLKLEVPGLKQEDFDIQLENNTLTVRGERKFEKEEKEENFHRIERRYGSFFRSFTVPTTVNHEGVKAGYDAGVLRIELEKRAEAK 139          
The following BLAST results are available for this feature:
BLAST of NO20G02130 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes)
Total hits: 20
Match NameE-valueIdentityDescription
EWM22022.12.500e-3759.09heat shock protein hsp20 [Nannochloropsis gaditana... [more]
EWM28800.11.000e-1438.05heat shock protein hsp20 [Nannochloropsis gaditana... [more]
OGO72027.17.300e-1340.82heat-shock protein Hsp20 [Chloroflexi bacterium RB... [more]
OQY88376.19.500e-1338.24hypothetical protein B6D39_11330 [Anaerolineae bac... [more]
PKL36239.19.500e-1348.42hypothetical protein CVV44_18670 [Spirochaetae bac... [more]
OGP94089.12.100e-1242.86molecular chaperone [Deltaproteobacteria bacterium... [more]
EPZ32380.13.600e-1240.20Alpha crystallin/Hsp20 domain-containing protein [... [more]
WP_015897492.16.200e-1243.68Hsp20/alpha crystallin family protein [Acidobacter... [more]
KDD76497.18.000e-1246.32hypothetical protein H632_c205p1 [Helicosporidium ... [more]
WP_050058308.18.000e-1242.16Hsp20/alpha crystallin family protein [Silvibacter... [more]

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Relationships

This gene is member of the following syntenic_region feature(s):

Feature NameUnique NameSpeciesType
nonsL021nonsL021Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ncniR020ncniR020Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ngnoR067ngnoR067Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region


This gene is orthologous to the following gene feature(s):

Feature NameUnique NameSpeciesType
NSK002758NSK002758Nannochloropsis salina (N. salina CCMP1776)gene


The following polypeptide feature(s) derives from this gene:

Feature NameUnique NameSpeciesType
NO20G02130.1NO20G02130.1-proteinNannochloropsis oceanica (N. oceanica IMET1)polypeptide
NO20G02130.2NO20G02130.2-proteinNannochloropsis oceanica (N. oceanica IMET1)polypeptide


The following gene feature(s) are orthologous to this gene:

Feature NameUnique NameSpeciesType
jgi.p|Nanoce1779_2|640395gene_6436Nannochloropsis oceanica (N. oceanica CCMP1779)gene
Naga_100959g2gene8413Nannochloropsis gaditana (N. gaditana B-31)gene


The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
NO20G02130.1NO20G02130.1Nannochloropsis oceanica (N. oceanica IMET1)mRNA
NO20G02130.2NO20G02130.2Nannochloropsis oceanica (N. oceanica IMET1)mRNA


Sequences
The following sequences are available for this feature:

gene sequence

>NO20G02130 ID=NO20G02130|Name=NO20G02130|organism=Nannochloropsis oceanica|type=gene|length=1701bp
GTTTGTTTGTTTGTTTGTTTGTTTTGTGAGTGTATGGCATGGCTTTCTTT
CACCCCCGGGACCTTTAATGAAAGCACGACATAGTTTGTAATCTTTTTCC
CCCAGACACCACATGACTGGGCACACATTTTCCCAGCCTTTCCTTCTGTC
CTGGTGTGCCTATTCTGTGTATCTTTTGGACCAACCGAATGTTCTGTTCT
ACATTGTGCATGTGTAGGCTGTCTCATTGACTTCGTGCTCCTCGCCACCC
ACACTCACGTCCTATACATTGTACCATCGCGCCATAGCACCGCCCCTCGT
TTGTTGGGCTTTTGTCGCAAGACGGAGACAGACACGATTTTACTCCCACA
AGCCACTATGGCTCCAGAAATACCCCCACACCACACGGGTGTGTCTTCTC
ACTGGACGTCCACAGCACCCACGGGCGTCTCATTTCTTCCCTTTCCCTCC
CTTTCATTTCCCGTGCACATGCCCATGCCCACCACAGCAGAACCCGAAGT
CTCAAGACTCAAGAACAAATCAACCAAGCCTACAACCCAACAACCATGGC
TCTCTCTCGCTTCGACCCCTTTGGATCCATGCTCACCGAGCGCGACCCGT
TTGCCGACTTCTTTAGCACCGGAGgtaagtattcaggagggaaggaaggt
ggaagtggagaggaggcggcggtagacagggatggatggcgggaggaaga
tcttgaaactgaaggaagaggtcacctttctttctatagctgaccaggca
ccggcctcacatccatcttccacctctcctacacccacctaccctctctt
tctacacagGCGGGTGGCTTGCCCCCAGCGGCGTGGGAGGCGGGGCTTTG
ACCAGTGGTGGCATGGGCCGCGGACAAGCTATCGTGCCCAACCTGAGCCT
GGATGTGAAGGAGAAAAACAACGAGTTTGAGGTCGCTGTTGATGCGCCGG
GCATGAAGAAGGAAGgtatgttggcgttgtggctgaagggagtggtgagg
atgctgtcttgcctgttgtcttccttctgtcgttctttccttatatcctt
acacccaccctcgctttctcccttccactctgcttccagACTTCGATGTG
ACGTTCGAGAACAACGTTCTGCGTGTGGCGTGTGAGCGCAAGGAGGAGAA
GGAGGAAGAGGACGCACGTTGGCACATTTCCGAGCGGCGCTGGGGCTCTT
GCTCCCGCTCTGTTCGCCTTCCTCGTGCCGCTGGCTGCGAGAACATCAAG
GCCGAATACGACGCGGGCGTTCTTAAGgtaaagaggatgagagggaagaa
ggcagagagggagggagggaagaagggagagagacaggatgaagcctttg
attctcacaccactttcgaattctttcctcccattccctctttccccccc
tctagATCCACGTGCCTAAGACGCAGAAGAGCGAGACCCGCAAGGCCATT
CGTGTGGAGTAGAGAAGAGAATGAGAGAAGAGAGAGAGAGAGAGGGGAGA
GAGAGAAAGAGAGAGGGAGAGGGAGAGGGCGCAGGGGGAAGGGAGGAGAA
AAATCTTCGAGTTCCCAGTCCAGAGAGTGTGCAGTGTAAAACTCGTGGCC
TAGATTTGTAAACCCCTCCTAAAAGTGAAGGAAAATATTACATAGTCCTC
CTTGTACGTAGTGAGCGAGAAGAAAAGAGATCAATCGGGGAAGTAGAAGT
A
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protein sequence of NO20G02130.1

>NO20G02130.1-protein ID=NO20G02130.1-protein|Name=NO20G02130.1|organism=Nannochloropsis oceanica|type=polypeptide|length=160bp
MALSRFDPFGSMLTERDPFADFFSTGGGWLAPSGVGGGALTSGGMGRGQA
IVPNLSLDVKEKNNEFEVAVDAPGMKKEDFDVTFENNVLRVACERKEEKE
EEDARWHISERRWGSCSRSVRLPRAAGCENIKAEYDAGVLKIHVPKTQKS
ETRKAIRVE*
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protein sequence of NO20G02130.2

>NO20G02130.2-protein ID=NO20G02130.2-protein|Name=NO20G02130.2|organism=Nannochloropsis oceanica|type=polypeptide|length=245bp
MALSRFDPFGSMLTERDPFADFFSTGGGWLAPSGVGGGALTSGGMGRGQA
IVPNLSLDVKEKNNEFEVAVDAPGMKKEDFDVTFENNVLRVACERKEEKE
EEDARWHISERRWGSCSRSVRLPRAAGCENIKAEYDAGVLKVKRMRGKKA
EREGGKKGERQDEAFDSHTTFEFFPPIPSFPPSRSTCLRRRRARPARPFV
WSREENERRERERGEREKERGRGRGRRGKGGEKSSSSQSRECAV*
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Synonyms
Publications