NO20G00510, NO20G00510 (gene) Nannochloropsis oceanica

BLAST of NO20G00510 vs. NCBI_GenBank
Match: EWM22164.1 (sterol esterase tgl1, partial [Nannochloropsis gaditana])

HSP 1 Score: 414.1 bits (1063), Expect = 6.800e-112
Identity = 229/392 (58.42%), Postives = 253/392 (64.54%), Query Frame = 0

Query:    4 YTDVLIDWAHLLYLVCFLIMAKAVGFFIVTVYAIFPFPSLLDRFYLASRGLQTWFSFQLGGAAPTGSKNGGRGRGHDRQLAESLVENTGFALETHTAPTKDGFVLVLHRLVPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNGSRTGTSGRPAVLLMHALMQDSESFLCGGKEHSLALYLAKSGFDVWLGNNRGNRYSHKHLRLSPSEDAYWNFSIDELARYDVPALIEYVLAATKQEKLCYVGFSQGSAQAFAAFTTNHALTQKVSLFLALAAPPRAKGLSSSYLSALVESNPRFIYLVFGRHSMLSSTLMWMKI 396
            YTDVL+DWAHLLYLVCFL  AKAVGFFIVTVYA FPFP+LLDRFYLAS+GLQ+WFS+QLGGAAP  +         +R LAESLVE T F LETHTA T+DGFVLVLHRLVP                                                                                                         N     T  RP +L++HALMQDSESFLCGG+EHSLAL LAK+GFDVWLGNNRGNRYSHKH+ LSPS+DAYWNFSIDELAR+DVPA+++YVL AT QEKL YVGFSQGSAQAFAAFTT+ A+ +KVS+FLALAAPPRAKGLSSSYLSALVESNPRFIYLVFGRHSMLSSTLMWMK+
Sbjct:   37 YTDVLVDWAHLLYLVCFLTAAKAVGFFIVTVYAYFPFPALLDRFYLASQGLQSWFSYQLGGAAPGSAGKASPNGNRERLLAESLVEGTPFILETHTALTRDGFVLVLHRLVP------------KTSGQDGPGAPGQATSSSTSETYVEDRGPQRARSWRKASGENNGRKSGGGSQSLQQHIPTSSFFSKGPGSAAAPAVAPALPSAAVPTATGTGSNAVTPSTLRRPPLLMVHALMQDSESFLCGGEEHSLALVLAKAGFDVWLGNNRGNRYSHKHVSLSPSQDAYWNFSIDELARFDVPAMVDYVLRATGQEKLAYVGFSQGSAQAFAAFTTDLAMAKKVSIFLALAAPPRAKGLSSSYLSALVESNPRFIYLVFGRHSMLSSTLMWMKV 416          

BLAST of NO20G00510 vs. NCBI_GenBank
Match: CBJ32519.1 (lipase [Ectocarpus siliculosus])

HSP 1 Score: 356.7 bits (914), Expect = 1.300e-94
Identity = 173/307 (56.35%), Postives = 229/307 (74.59%), Query Frame = 0

Query:  230 RPAVLLMHALMQDSESFLCGGKEHSLALYLAKSGFDVWLGNNRGNRYSHKHLRLSP-SEDAYWNFSIDELARYDVPALIEYVLAATKQEKLCYVGFSQGSAQAFAAFTTNHALTQKVSLFLALAAPPRAKGLSSSYLSALVESNPRFIYLVFGRHSMLSSTLMWMKIMSPQLFVWTVDRATDFLFNWSNSQVSATRKVHLYQHIFSYASVKTVVQWFQLIKLRRLAFYADDTNSNRVPVEYDLKRLRMPVAVFYGGKDTVIDIDALKEVLPACVKWHEEPLYEHLDMIWADTAKDVIFPEVMKLLEE 536
            RPAVLLMH LMQDSE+ LCGG++H+L L LA +GFDV+LGN RGNRYSHKHLR SP + DAYW+FSIDELARYD P +I++VL  + Q +LCY+GFSQG+AQAFAAF+++  L  KV LF+AL+   RA  LS S L +LV++N RFIYL+FGR  ML   L W ++MS + FV T+D A  +LF+WS S+VSA RK+ +Y HI+S+ SVK V+ WFQ++   RL+ +AD   S  VPV YD+ R+  PVA+ YGG+D ++++++L E+LP  V    E  YEHLD +WADTAKD +FP+V  L+ +
Sbjct:   67 RPAVLLMHGLMQDSEALLCGGRDHALGLRLAAAGFDVFLGNARGNRYSHKHLRRSPVTSDAYWDFSIDELARYDTPTMIDHVLKVSGQTQLCYIGFSQGTAQAFAAFSSDPDLQAKVFLFVALSPAVRANHLSKSLLLSLVQANLRFIYLLFGRRRMLPVALTWQRMMSREFFVATIDGAISYLFSWSASEVSAKRKLEVYPHIYSFISVKCVLHWFQMMYSGRLSMFADGPASQYVPVTYDVARVSCPVAIVYGGRDHLVEVESLIEILPNVVLSIREDKYEHLDTMWADTAKDTVFPKVETLVRQ 373          

BLAST of NO20G00510 vs. NCBI_GenBank
Match: XP_012758971.1 (hypothetical protein SAMD00019534_000840, partial [Acytostelium subglobosum LB1] >GAM16909.1 hypothetical protein SAMD00019534_000840, partial [Acytostelium subglobosum LB1])

HSP 1 Score: 283.5 bits (724), Expect = 1.400e-72
Identity = 146/324 (45.06%), Postives = 202/324 (62.35%), Query Frame = 0

Query:  228 SGRPAVLLMHALMQDSESFLC-GGKEHSLALYLAKSGFDVWLGNNRGNRYSHKHLRLSPSEDAYWNFSIDELARYDVPALIEYVLAATKQEKLCYVGFSQGSAQAFAAFTTNHALTQKVSLFLALAAPPRAKGLSSSYLSALVESNPRFIYLVFGRHSMLSSTLMWMKIMSPQLFVWTVDRATDFLFNWSNSQVSATRKVHLYQHIFSYASVKTVVQWFQLIKLRRLAFYAD-------DTNSN-------RVPVEYDLKRLRMPVAVFYGGKDTVIDIDALKEVLP--ACVKWHEEPLYEHLDMIWADTAKDVIFPEVMKLLE 535
            SG+PAVL+MH  MQ SE++LC      SL   LA +G+DVWLGNNRGN+YS KH  LSP ++ +WN+S+DEL RYD+P+++++++  TK + L Y+GFSQG+AQ +AA +TN +L  KV LF+ALA     KG S+  + +L  S P FI+L+FG+ SML ST  W   +    FV  +D A +FLFNW  + +S   K  LY HIFS +SVK+VV WFQ+I+  R   + D       + NSN       RV   Y   ++    A+F+GG DT+ +  AL   LP    V  HEE  YEHLD +WA  A   IF +++KL++
Sbjct:  106 SGKPAVLIMHGFMQTSEAWLCRSDPNDSLPFILADAGYDVWLGNNRGNKYSFKHTTLSPDQEKFWNWSLDELVRYDLPSIVDFIIDRTKLKSLTYIGFSQGTAQGWAALSTNTSLASKVDLFIALAPVATVKGFSNPMIDSLARSRPDFIFLLFGKKSMLPSTTFWRNTLPKHFFVACIDWAINFLFNWQTTNLSPEEKPMLYSHIFSTSSVKSVVHWFQIIQTNRFQMFDDMLLGITPNENSNQPHRYHGRVIPGYHPGQILTKCALFFGGADTLPNTKALLRHLPRDKVVFVHEEETYEHLDFMWAKDANKKIFSKILKLMK 429          

BLAST of NO20G00510 vs. NCBI_GenBank
Match: OJD19322.1 (hypothetical protein AJ78_00681 [Emergomyces pasteuriana Ep9510])

HSP 1 Score: 282.7 bits (722), Expect = 2.300e-72
Identity = 136/322 (42.24%), Postives = 201/322 (62.42%), Query Frame = 0

Query:  226 GTSGRPAVLLMHALMQDSESFLC-GGKEHSLALYLAKSGFDVWLGNNRGNRYSHKHLRLSPSEDAYWNFSIDELARYDVPALIEYVLAATKQEKLCYVGFSQGSAQAFAAFTTNHALTQKVSLFLALAAPPRAKGLSSSYLSALVESNPRFIYLVFGRHSMLSSTLMWMKIMSPQLFVWTVDRATDFLFNWSNSQVSATRKVHLYQHIFSYASVKTVVQWFQLIKLRRLAFYADD-------TNSNRV--PVEYDLKRLRMPVAVFYGGKDTVIDIDALKEVLPACVKWHEEPLYEHLDMIWADTAKDVIFPEVMKLLEEYG 538
            GT  +P V L H L+ +SE ++C   +E  L   L + G+DVWLGNNRGN+YS K +  +PS  A+WNFS+D+ A +D+P  IEY+L+ T Q  L YVGFSQG+AQAFA  + +  L QK++LF+ALA      GLS+  + AL++++P  ++L FGR S+LSST MW  I+ P +FV  +D +  FLFNWS   ++  +K+  Y H++S+ S K+VV WFQ+I+ +    Y DD        N+NR   P ++  K ++ P+ + YGG+D+++DID + E LP          YEHLD +WA+   D +FP +   L  +G
Sbjct:  127 GTPQKPVVYLHHGLLMNSEVWVCLTNEERCLPFQLVEKGYDVWLGNNRGNKYSKKSIFHAPSSTAFWNFSMDQFAFHDIPDSIEYILSVTSQAALSYVGFSQGTAQAFATLSIHPTLNQKINLFIALAPAMSPAGLSNGVVDALMKASPNVLFLAFGRRSILSSTPMWQTILYPPIFVRIIDISLSFLFNWSAVNINTDQKLAAYPHLYSFTSTKSVVHWFQIIRNKSFQMYDDDAGPSVSINNNNRYYKPAKFPTKNIKTPIVLVYGGQDSLVDIDVMLEELPGHTVATSISHYEHLDFLWANDVADQVFPHIFDALGSHG 448          

BLAST of NO20G00510 vs. NCBI_GenBank
Match: XP_001543627.1 (hypothetical protein HCAG_00673 [Histoplasma capsulatum NAm1] >EDN02809.1 hypothetical protein HCAG_00673 [Histoplasma capsulatum NAm1])

HSP 1 Score: 282.3 bits (721), Expect = 3.100e-72
Identity = 135/322 (41.93%), Postives = 202/322 (62.73%), Query Frame = 0

Query:  226 GTSGRPAVLLMHALMQDSESFLC-GGKEHSLALYLAKSGFDVWLGNNRGNRYSHKHLRLSPSEDAYWNFSIDELARYDVPALIEYVLAATKQEKLCYVGFSQGSAQAFAAFTTNHALTQKVSLFLALAAPPRAKGLSSSYLSALVESNPRFIYLVFGRHSMLSSTLMWMKIMSPQLFVWTVDRATDFLFNWSNSQVSATRKVHLYQHIFSYASVKTVVQWFQLIKLRRLAFYADD-------TNSNRV--PVEYDLKRLRMPVAVFYGGKDTVIDIDALKEVLPACVKWHEEPLYEHLDMIWADTAKDVIFPEVMKLLEEYG 538
            G   +P V L H L+ +SE ++C   +E  L   L + G+DVWLGNNRGN+YS K +  SPS  A+W+FSID+ A +D+P  IEY+L+ T Q+ L Y+GFSQG+AQAFA  + +  L QK++LF+ALA      GLS+  + AL++++P  ++L FGR S+LSST MW  I+ P +FV  +D +  FLFNWS+  +++ +K+  Y H+FS+ S K+VV WFQ+I+ +    Y DD        N++R   P ++  K ++ P+ + YGG+D+++DID +   LP        P YEHLD +WA    D +FP +   L  +G
Sbjct:  127 GARQKPVVYLHHGLLMNSEVWVCLTNEERCLPFQLVEKGYDVWLGNNRGNKYSKKSIFHSPSSTAFWDFSIDQFAFHDIPDSIEYILSVTSQKSLSYIGFSQGTAQAFATLSIHPTLNQKINLFIALAPAMSPAGLSNGVVDALMKASPNVLFLAFGRRSILSSTPMWQTILYPPIFVRIIDISLSFLFNWSSVNINSDQKLAAYPHLFSFTSTKSVVHWFQIIRNKSFQMYDDDAGPLVNINNNSRYYKPAKFPTKNIKTPIVLVYGGRDSLVDIDVMLGELPNHTLATSIPHYEHLDFLWARDVADQVFPHIFDALRSHG 448          

BLAST of NO20G00510 vs. NCBI_GenBank
Match: KLJ05888.1 (lysosomal acid lipase/cholesteryl ester hydrolase [Emmonsia parva UAMH 139])

HSP 1 Score: 282.0 bits (720), Expect = 4.000e-72
Identity = 134/322 (41.61%), Postives = 203/322 (63.04%), Query Frame = 0

Query:  226 GTSGRPAVLLMHALMQDSESFLC-GGKEHSLALYLAKSGFDVWLGNNRGNRYSHKHLRLSPSEDAYWNFSIDELARYDVPALIEYVLAATKQEKLCYVGFSQGSAQAFAAFTTNHALTQKVSLFLALAAPPRAKGLSSSYLSALVESNPRFIYLVFGRHSMLSSTLMWMKIMSPQLFVWTVDRATDFLFNWSNSQVSATRKVHLYQHIFSYASVKTVVQWFQLIKLRRLAFYADD-------TNSNRV--PVEYDLKRLRMPVAVFYGGKDTVIDIDALKEVLPACVKWHEEPLYEHLDMIWADTAKDVIFPEVMKLLEEYG 538
            GT  +P V L H L+ +SE ++C   +E  L   L + G+DVW GNNRGN+YS K +  +PS  A+W+FS+DE A +D+P  IEY+L+ T Q+ L Y+GFSQG+AQAFA  + +  L QK++LF+ALA      GLS+  + AL++++P  ++L FGR S+LSST MW  I+ P +FV  +D +  FLFNWS   +++ +K+  Y H++S+ S K+VV WFQ+I+ +    Y DD        N+NR   P ++  + ++ P+ + YGG+D+++DID + E LP+  +      YEHLD +WA    D  FP V+  L  +G
Sbjct:  127 GTRQKPVVYLHHGLLMNSEVWVCLTDEERCLPFQLVEKGYDVWFGNNRGNKYSKKSVSHAPSSTAFWDFSMDEFAFHDIPDSIEYILSVTSQKSLSYIGFSQGTAQAFATLSIHPTLNQKINLFIALAPAMSPAGLSNGVVDALMKASPNVLFLAFGRRSILSSTPMWQTILYPPIFVRIIDISLSFLFNWSGVNINSDQKLAAYPHLYSFTSTKSVVHWFQIIRNKSFQMYDDDAGPSVSINNNNRYYKPAKFPTRNIKTPIVLVYGGRDSLVDIDVMLEELPSHTQAISISHYEHLDFLWARDVADQAFPHVLDALRSHG 448          

BLAST of NO20G00510 vs. NCBI_GenBank
Match: PGH36600.1 (esterase/lipase [Emmonsia crescens])

HSP 1 Score: 281.6 bits (719), Expect = 5.200e-72
Identity = 135/322 (41.93%), Postives = 201/322 (62.42%), Query Frame = 0

Query:  226 GTSGRPAVLLMHALMQDSESFLC-GGKEHSLALYLAKSGFDVWLGNNRGNRYSHKHLRLSPSEDAYWNFSIDELARYDVPALIEYVLAATKQEKLCYVGFSQGSAQAFAAFTTNHALTQKVSLFLALAAPPRAKGLSSSYLSALVESNPRFIYLVFGRHSMLSSTLMWMKIMSPQLFVWTVDRATDFLFNWSNSQVSATRKVHLYQHIFSYASVKTVVQWFQLIKLRRLAFYADD-------TNSNRV--PVEYDLKRLRMPVAVFYGGKDTVIDIDALKEVLPACVKWHEEPLYEHLDMIWADTAKDVIFPEVMKLLEEYG 538
            GT  +P V L H L+ +SE ++C   +E  L   L + G+DVWLGNNRGN+YS K +  +PS  A+WNFS+D+ A +D+P  IEY+L+ T Q+ L Y+GFSQG+AQAFA  + +  L QK++LF+ALA      GLS+  + AL++++P  ++L FGR S+LSST MW  I+ P +FV  +D +  FLFNWS   ++  +K+  Y H++S+ S K+VV WFQ+I+ R    Y DD        N+NR   P ++  K ++ P+ + YGG+D+++DI+ + E LP          YEHLD +WA    D +FP +   L  +G
Sbjct:  127 GTPQKPVVYLHHGLLMNSEVWVCLTDEERCLPFQLVEKGYDVWLGNNRGNKYSKKSISHAPSSTAFWNFSMDQFAFHDIPDSIEYILSVTSQKALSYIGFSQGTAQAFATLSIHPTLNQKINLFIALAPAMSPAGLSNGVVDALMKASPNVLFLAFGRRSILSSTPMWQTILYPPIFVRIIDMSLSFLFNWSGVNINNDQKLAAYPHLYSFTSTKSVVHWFQIIRNRSFQMYDDDAGPSVSINNNNRYYKPAKFPTKNIKTPIVLVYGGQDSLVDINVMLEELPNHTVATSISHYEHLDFLWARDVADQVFPHIFDALGSHG 448          

BLAST of NO20G00510 vs. NCBI_GenBank
Match: KKZ59048.1 (lysosomal acid lipase/cholesteryl ester hydrolase [Emmonsia crescens UAMH 3008])

HSP 1 Score: 281.2 bits (718), Expect = 6.800e-72
Identity = 134/322 (41.61%), Postives = 201/322 (62.42%), Query Frame = 0

Query:  226 GTSGRPAVLLMHALMQDSESFLC-GGKEHSLALYLAKSGFDVWLGNNRGNRYSHKHLRLSPSEDAYWNFSIDELARYDVPALIEYVLAATKQEKLCYVGFSQGSAQAFAAFTTNHALTQKVSLFLALAAPPRAKGLSSSYLSALVESNPRFIYLVFGRHSMLSSTLMWMKIMSPQLFVWTVDRATDFLFNWSNSQVSATRKVHLYQHIFSYASVKTVVQWFQLIKLRRLAFYADD-------TNSNRV--PVEYDLKRLRMPVAVFYGGKDTVIDIDALKEVLPACVKWHEEPLYEHLDMIWADTAKDVIFPEVMKLLEEYG 538
            GT  +P V L H L+ +SE ++C   +E  L   L + G+DVWLGNNRGN+YS K +  +PS  A+WNFS+D+ A +D+P  IEY+L+ T Q+ L Y+GFSQG+AQAFA  + +  L QK++LF+ALA      GLS+  + AL++++P  ++L FGR S+LSST MW  I+ P +FV  +D +  FLFNWS   ++  +K+  Y H++S+ S K+VV WFQ+I+ R    Y DD        N+NR   P ++  K ++ P+ + YGG+D+++DI+ + E LP+         YEHLD +W     D +FP +   L  +G
Sbjct:  127 GTPQKPVVYLHHGLLMNSEVWVCLTDEERCLPFQLVEKGYDVWLGNNRGNKYSKKSISHAPSSTAFWNFSMDQFAFHDIPDSIEYILSVTSQKTLSYIGFSQGTAQAFATLSIHPTLNQKINLFIALAPAMSPDGLSNGVVDALMKASPNVLFLAFGRRSILSSTPMWQTILYPPIFVRIIDMSLSFLFNWSGVNINNDQKLAAYPHLYSFTSTKSVVHWFQIIRNRSFQMYDDDAGPSVSINNNNRYYKPAKFPTKNIKTPIVLVYGGQDSLVDINVMLEELPSHTVATSISHYEHLDFLWGRDVADQVFPHIFDALGSHG 448          

BLAST of NO20G00510 vs. NCBI_GenBank
Match: EEH02790.1 (triacylglycerol lipase [Histoplasma capsulatum G186AR])

HSP 1 Score: 280.0 bits (715), Expect = 1.500e-71
Identity = 134/322 (41.61%), Postives = 202/322 (62.73%), Query Frame = 0

Query:  226 GTSGRPAVLLMHALMQDSESFLC-GGKEHSLALYLAKSGFDVWLGNNRGNRYSHKHLRLSPSEDAYWNFSIDELARYDVPALIEYVLAATKQEKLCYVGFSQGSAQAFAAFTTNHALTQKVSLFLALAAPPRAKGLSSSYLSALVESNPRFIYLVFGRHSMLSSTLMWMKIMSPQLFVWTVDRATDFLFNWSNSQVSATRKVHLYQHIFSYASVKTVVQWFQLIKLRRLAFYADD-------TNSNRV--PVEYDLKRLRMPVAVFYGGKDTVIDIDALKEVLPACVKWHEEPLYEHLDMIWADTAKDVIFPEVMKLLEEYG 538
            G   +P V L H L+ +SE ++C   +E  L   L + G+DVWLGNNRGN+YS K +  SPS  A+W+FSID+ A +D+P  IEY+L+ T Q+ L Y+GFSQG+AQAFA  + +  L QK++LF+ALA      GLS+  + AL++++P  ++L FGR S+LSST MW  I+ P +FV  +D +  FLFNWS+  +++ +K+  Y H+FS+ S K+VV WFQ+I+ +    Y DD        N++R   P ++  K ++ P+ + YGG+D+++DID +   LP          YEHLD +WA    D +FP ++  L  +G
Sbjct:  127 GARQKPVVYLHHGLLMNSEVWVCLTNEERCLPFQLVEKGYDVWLGNNRGNKYSKKSIFHSPSSTAFWDFSIDQFAFHDIPDSIEYILSVTSQKSLSYIGFSQGTAQAFATLSIHPTLNQKINLFIALAPAMSPAGLSNGVVDALMKASPNVLFLAFGRRSILSSTPMWQTILYPPIFVRIIDISLSFLFNWSSVNINSDQKLAAYPHLFSFTSTKSVVHWFQIIRNKSFQMYDDDAGPLVNINNNSRYYKPAKFPTKNIKTPIVLVYGGRDSLVDIDVMLGELPNHTLATSISHYEHLDFLWARDVADQVFPHILDALRSHG 448          

BLAST of NO20G00510 vs. NCBI_GenBank
Match: PGH04954.1 (esterase/lipase [Emmonsia parva])

HSP 1 Score: 279.6 bits (714), Expect = 2.000e-71
Identity = 132/326 (40.49%), Postives = 204/326 (62.58%), Query Frame = 0

Query:  222 GSRTGTSGRPAVLLMHALMQDSESFLC-GGKEHSLALYLAKSGFDVWLGNNRGNRYSHKHLRLSPSEDAYWNFSIDELARYDVPALIEYVLAATKQEKLCYVGFSQGSAQAFAAFTTNHALTQKVSLFLALAAPPRAKGLSSSYLSALVESNPRFIYLVFGRHSMLSSTLMWMKIMSPQLFVWTVDRATDFLFNWSNSQVSATRKVHLYQHIFSYASVKTVVQWFQLIKLRRLAFYADD-------TNSNRV--PVEYDLKRLRMPVAVFYGGKDTVIDIDALKEVLPACVKWHEEPLYEHLDMIWADTAKDVIFPEVMKLLEEYG 538
            G+  GT  +P + L H L+ +SE ++C   +E  L   L + G+DVW GNNRGN+YS K +  +PS  A+W+FS+DE A +D+P  IEY+L+ T Q+ L Y+GFSQG+AQAFA  + +  L  K++LF+ALA      GL++  + AL++++P  ++L FGR S+LSST MW  I+ P +FV  +D +  FLFNW+   +++ +K+  Y H++S+ S K+VV WFQ+I+ +    Y DD        N++R   P ++  K ++ P+ + YGG+D+++DID + E LP+  +    P YEHLD +WA    D  FP V   L  +G
Sbjct:  123 GADDGTHQKPVIYLHHGLLMNSEVWVCLTDEERCLPFQLVEKGYDVWFGNNRGNKYSKKSVSHAPSTTAFWDFSMDEFAFHDIPDSIEYILSVTSQKALSYIGFSQGTAQAFATLSIHPTLNHKINLFIALAPAMSPAGLANGVVDALMKASPNVLFLAFGRRSILSSTPMWQTILYPPIFVRIIDISLSFLFNWTGVNINSDQKLAAYPHLYSFTSTKSVVHWFQIIRNKSFQMYDDDAGPSVSVNNNDRYYKPAKFPTKNIKTPIVLVYGGRDSLVDIDVMLEELPSHTQAISIPHYEHLDFLWARDVADQAFPHVFDALRSHG 448