NO18G01840, NO18G01840 (gene) Nannochloropsis oceanica

BLAST of NO18G01840 vs. NCBI_GenBank
Match: EWM23389.1 (aspartate-semialdehyde dehydrogenase [Nannochloropsis gaditana])

HSP 1 Score: 575.5 bits (1482), Expect = 1.400e-160
Identity = 277/325 (85.23%), Postives = 308/325 (94.77%), Query Frame = 0

Query:   86 DRRFPVTLDTLRLFASERSAGKKIKTPYGDKKIELFSVESARECDFVFLAVSGTFALQYAKKISEKGGPYVIDNSSAFRYDDDIPLVVPEINSAAIGASRLIANPNCTTAIAVMALWPLHQQFGLKRILCSTYQAASGAGQEGMEELRTGMEHYLIGREYKNEVFAHPLPFNVIPHIDKFQANGYTKEEMKVAWETKKIFDYPHMDIACTAVRVPTLRAHAESITIETYLDIEPSAALFALRKAPGVEVRDSGEENLYPMPLTATGKYDVEVGRVRQSLVFGSKGLELFVCGDQLLRGAASNAVFIAEAVLFPERYRGGRGEGTG 411
            DRRFPVTL+TLRLFASERSAGK + TPYG+KKIELFSVE+ARECDFVFLAVSGTFALQYAKKISEKGGPYVIDNSSAFRYDDDIPLVVPEIN+AAIG+S+LIANPNCTTAIAVMALWPLH+++G+KR+L STYQAASGAGQEGMEELRTGMEHYLIGR+ +++VFAHPLPFNVIPHIDKFQ NGYTKEEMKVAWET+KIFDYPHM+++CTAVRVPTLRAHAE++TIETY +IEPS AL  L++APGVEVRD GE+NLYPMPLTATGKY+VEVGRVRQSLVFG KGL++FVCGDQLLRGAASNAVFIAEAVL P++Y  GR  G G
Sbjct:   32 DRRFPVTLETLRLFASERSAGKTLSTPYGEKKIELFSVEAARECDFVFLAVSGTFALQYAKKISEKGGPYVIDNSSAFRYDDDIPLVVPEINAAAIGSSKLIANPNCTTAIAVMALWPLHRRYGIKRLLVSTYQAASGAGQEGMEELRTGMEHYLIGRDCQHQVFAHPLPFNVIPHIDKFQENGYTKEEMKVAWETRKIFDYPHMEVSCTAVRVPTLRAHAEALTIETYQEIEPSQALLLLQEAPGVEVRDDGEKNLYPMPLTATGKYEVEVGRVRQSLVFGRKGLDMFVCGDQLLRGAASNAVFIAEAVLSPDKYGVGREGGWG 356          

BLAST of NO18G01840 vs. NCBI_GenBank
Match: PCH99771.1 (aspartate-semialdehyde dehydrogenase [Alphaproteobacteria bacterium])

HSP 1 Score: 401.4 bits (1030), Expect = 3.600e-108
Identity = 201/302 (66.56%), Postives = 240/302 (79.47%), Query Frame = 0

Query:   96 LRLFASERSAGKKIKTPYGDKKIELFSVESARECDFVFLAVSGTFALQYAKKISEKGGPYVIDNSSAFRYDDDIPLVVPEIN-SAAIGASRLIANPNCTTAIAVMALWPLHQQFGLKRILCSTYQAASGAGQEGMEELRTGMEHYLIGREYKNEVFAHPLPFNVIPHIDKFQANGYTKEEMKVAWETKKIFDYPHMDIACTAVRVPTLRAHAESITIETYLDIEPSAALFALRKAPGVEVRDSGEENLYPMPLTATGKYDVEVGRVRQSLVFGSKGLELFVCGDQLLRGAASNAVFIAEAVL 397
            +RLFAS RSAGK+I TP+G+  IE FSV +A+ECD V +AVSG FA ++A+ I++K    VIDNSSAFRY DDIPLV+PEIN  AA+GAS LIANPNCTTAIA +ALWPLHQ FG+KR + STYQAASGAG  GM+EL TG+  +  G + +   F HPLPFNVIPHID FQANGYT+EEMKV WET+KIF  P + I+CTAVR+PTLRAHAE+ITIET   +  ++A  AL  APG+EV D    N YPMPLTATGKYDVEVGR+RQ+ VFG+ GL++FVCGDQLL+GAA NAV IAE +L
Sbjct:   17 VRLFASARSAGKQIPTPFGELTIEEFSVSAAQECDIVLMAVSGGFATEHAETIAQKS--VVIDNSSAFRYRDDIPLVIPEINPQAAVGAS-LIANPNCTTAIAAVALWPLHQAFGIKRAIISTYQAASGAGSPGMDELETGIRAWSRGDDIQASTFQHPLPFNVIPHIDSFQANGYTREEMKVTWETRKIFGLPELPISCTAVRIPTLRAHAETITIETLSPVTTASARAALENAPGIEVVDDTSANFYPMPLTATGKYDVEVGRIRQNPVFGNHGLDIFVCGDQLLKGAALNAVQIAELLL 315          

BLAST of NO18G01840 vs. NCBI_GenBank
Match: KJS38848.1 (aspartate-semialdehyde dehydrogenase [Rhodospirillaceae bacterium BRH_c57])

HSP 1 Score: 400.6 bits (1028), Expect = 6.100e-108
Identity = 199/311 (63.99%), Postives = 240/311 (77.17%), Query Frame = 0

Query:   86 DRRFPVTLDTLRLFASERSAGKKIKTPYGDKKIELFSVESARECDFVFLAVSGTFALQYAKKISEKGGPYVIDNSSAFRYDDDIPLVVPEINSAAIGASRLIANPNCTTAIAVMALWPLHQQFGLKRILCSTYQAASGAGQEGMEELRTGMEHYLIGREYKNEVFAHPLPFNVIPHIDKFQANGYTKEEMKVAWETKKIFDYPHMDIACTAVRVPTLRAHAESITIETYLDIEPSAALFALRKAPGVEVRDSGEENLYPMPLTATGKYDVEVGRVRQSLVFGSKGLELFVCGDQLLRGAASNAVFIAEAVL 397
            DR FP  L  LRL+AS RSAGK I+TPYG K IE FSV++A ECD   +AVSG FA +YA +++E+ G  VIDNSSAFRYDDD PL+VPEIN  AIG +R+I+NPNCTTAI  +ALWPLH++FG+K+++ STYQAASGAG EGM+EL      +L   +  N VFAHPLPFN+IPHID FQ NGYTKEEMKV WE++KI   P + ++CTAVR+PT RAHAES  IET   I+P+AA   L KAPGV+V D     +YPMPL A+G+YDVEVGR+RQ+LVFG  GLE FVCGDQLL+GAA NAV IAE V+
Sbjct:   30 DRAFP--LSELRLYASARSAGKTIETPYGTKTIEAFSVDAAAECDIALMAVSGDFAKEYAPQLAER-GVTVIDNSSAFRYDDDKPLIVPEINPEAIGDARIISNPNCTTAILAVALWPLHKEFGVKKVIVSTYQAASGAGAEGMKELEDEARAFLSTGKAGNAVFAHPLPFNLIPHIDAFQDNGYTKEEMKVTWESRKIMGLPDLALSCTAVRIPTSRAHAESAVIETEKPIDPAAARAVLSKAPGVKVVDDPAAKVYPMPLNASGQYDVEVGRIRQNLVFGDHGLEFFVCGDQLLKGAALNAVQIAEVVI 337          

BLAST of NO18G01840 vs. NCBI_GenBank
Match: WP_051089907.1 (aspartate-semialdehyde dehydrogenase [Novispirillum itersonii])

HSP 1 Score: 396.4 bits (1017), Expect = 1.200e-106
Identity = 200/311 (64.31%), Postives = 233/311 (74.92%), Query Frame = 0

Query:   86 DRRFPVTLDTLRLFASERSAGKKIKTPYGDKKIELFSVESARECDFVFLAVSGTFALQYAKKISEKGGPYVIDNSSAFRYDDDIPLVVPEINSAAIGASRLIANPNCTTAIAVMALWPLHQQFGLKRILCSTYQAASGAGQEGMEELRTGMEHYLIGREYKNEVFAHPLPFNVIPHIDKFQANGYTKEEMKVAWETKKIFDYPHMDIACTAVRVPTLRAHAESITIETYLDIEPSAALFALRKAPGVEVRDSGEENLYPMPLTATGKYDVEVGRVRQSLVFGSKGLELFVCGDQLLRGAASNAVFIAEAVL 397
            DR FP  L  LRLFASERSAGK   TPYG K IE F+V++  ECD   LAVSG FA ++A +++ + G  VIDNSSAFRYDDD PLV+PEIN  A+G +R+IANPNCTTAI  +ALWPLHQ FGLK+++ STYQAASGAG EGM EL      YL   +  N+VFAHPLPFN+IPHID FQ NGYTKEEMKV WE++KI   P + ++CTAVR+PT RAHAES  IE    + P AA   L  APGV+V D     +YPMPL A+GKYDVEVGR+RQ+LVFG  GLELFVCGDQLL+GAA NAV IAE VL
Sbjct:   30 DRAFP--LSELRLFASERSAGKTTATPYGTKTIEAFTVDAVAECDIALLAVSGDFAKEFAPQLAAR-GVTVIDNSSAFRYDDDKPLVIPEINPEAVGDARIIANPNCTTAILAVALWPLHQAFGLKKVIVSTYQAASGAGAEGMSELEDQARAYLDTGKAANKVFAHPLPFNLIPHIDAFQDNGYTKEEMKVTWESRKIMGIPDLALSCTAVRIPTSRAHAESAVIEFAKPVTPDAARALLAAAPGVKVVDDPAAKVYPMPLNASGKYDVEVGRIRQNLVFGDHGLELFVCGDQLLKGAALNAVQIAEVVL 337          

BLAST of NO18G01840 vs. NCBI_GenBank
Match: WP_009541231.1 (aspartate-semialdehyde dehydrogenase [Caenispirillum salinarum] >EKV29266.1 Aspartate-semialdehyde dehydrogenase [Caenispirillum salinarum AK4])

HSP 1 Score: 396.0 bits (1016), Expect = 1.500e-106
Identity = 198/311 (63.67%), Postives = 239/311 (76.85%), Query Frame = 0

Query:   86 DRRFPVTLDTLRLFASERSAGKKIKTPYGDKKIELFSVESARECDFVFLAVSGTFALQYAKKISEKGGPYVIDNSSAFRYDDDIPLVVPEINSAAIGASRLIANPNCTTAIAVMALWPLHQQFGLKRILCSTYQAASGAGQEGMEELRTGMEHYLIGREYKNEVFAHPLPFNVIPHIDKFQANGYTKEEMKVAWETKKIFDYPHMDIACTAVRVPTLRAHAESITIETYLDIEPSAALFALRKAPGVEVRDSGEENLYPMPLTATGKYDVEVGRVRQSLVFGSKGLELFVCGDQLLRGAASNAVFIAEAVL 397
            DR FP+T   LRLFAS RSAGK ++TP+G K IE F+V+S  ECD   LAVSG F+ +YA +++E+ G  VIDNSSAFRYDDD PLVVPEIN+ AIG +++IANPNCTTAI  +AL PLH +FGLKR + STYQAASG+G  GM+EL     + L G +  N+VF HPLPFNVIPHIDKFQ NGYTKEEMKV WET+KI   P + ++CTAVR+PTLRAHAE+ T+E    + P AA  AL  APGV++ D  E N YPMPL A+G YDVEVGR+RQS+VFG  GL++FVCGDQLL+GAA NAV IAE V+
Sbjct:   30 DRNFPLT--DLRLFASARSAGKTLETPFGSKTIEEFTVDSVAECDVALLAVSGDFSKEYAPQLAER-GVTVIDNSSAFRYDDDKPLVVPEINADAIGDAKIIANPNCTTAILAVALQPLHAKFGLKRAIVSTYQAASGSGAPGMKELEEETRNILQGGQSSNKVFVHPLPFNVIPHIDKFQDNGYTKEEMKVTWETRKIMRIPDLPLSCTAVRIPTLRAHAEAATLEFDKPVTPEAAREALSGAPGVKIVDDPENNAYPMPLNASGHYDVEVGRIRQSIVFGDHGLDIFVCGDQLLKGAALNAVQIAECVV 337          

BLAST of NO18G01840 vs. NCBI_GenBank
Match: WP_085584560.1 (aspartate-semialdehyde dehydrogenase [Thalassospira mesophila] >OSQ37046.1 aspartate-semialdehyde dehydrogenase [Thalassospira mesophila])

HSP 1 Score: 395.2 bits (1014), Expect = 2.600e-106
Identity = 199/309 (64.40%), Postives = 234/309 (75.73%), Query Frame = 0

Query:   86 DRRFPVTLDTLRLFASERSAGKKIKTPYGDKKIELFSVESARECDFVFLAVSGTFALQYAKKISEKGGPYVIDNSSAFRYDDDIPLVVPEINSAAIGASRLIANPNCTTAIAVMALWPLHQQFGLKRILCSTYQAASGAGQEGMEELRTGMEHYLIGREYKNEVFAHPLPFNVIPHIDKFQANGYTKEEMKVAWETKKIFDYPHMDIACTAVRVPTLRAHAESITIETYLDIEPSAALFALRKAPGVEVRDSGEENLYPMPLTATGKYDVEVGRVRQSLVFGSKGLELFVCGDQLLRGAASNAVFIAEA 395
            DR FPV    L LFASERSAGK ++T YG K + LFSVE AR+CD VFLAVSG FA +YA +I+   G  VIDNSSAFR +DD+PL+VPEIN      ++LIANPNCTTAIA +ALWPLHQ FGLK++  STYQAASGAGQ GM ELR G+   L G E K EVF+HPL FNVIPHID FQ NGYT+EEMKV WET+KIF  P + I+CTAVR+PT R H+ESI +ET   + P AA   L KA GV++ D+ E+  YPMP+ AT +YDVEVGR+R +L+FG  GLELFV GDQLL+GAA NAV IAEA
Sbjct:   27 DRNFPV--GELTLFASERSAGKTLETKYGTKTVTLFSVEEARKCDIVFLAVSGDFAKEYAPQIAAGDGALVIDNSSAFRLNDDVPLIVPEINGDLAKTAKLIANPNCTTAIAAVALWPLHQAFGLKKVFVSTYQAASGAGQPGMNELREGLAAGLKGEEIKAEVFSHPLAFNVIPHIDTFQENGYTREEMKVTWETRKIFGVPDLAISCTAVRIPTERTHSESIVVETEKPVTPQAARDVLAKARGVKLVDNPEKLEYPMPINATKQYDVEVGRIRTNLIFGDHGLELFVAGDQLLKGAALNAVQIAEA 333          

BLAST of NO18G01840 vs. NCBI_GenBank
Match: WP_085615623.1 (aspartate-semialdehyde dehydrogenase [Thalassospira alkalitolerans] >OSQ50417.1 aspartate-semialdehyde dehydrogenase [Thalassospira alkalitolerans])

HSP 1 Score: 394.0 bits (1011), Expect = 5.700e-106
Identity = 198/311 (63.67%), Postives = 238/311 (76.53%), Query Frame = 0

Query:   86 DRRFPVTLDTLRLFASERSAGKKIKTPYGDKKIELFSVESARECDFVFLAVSGTFALQYAKKISEKGGPYVIDNSSAFRYDDDIPLVVPEINSAAIGASRLIANPNCTTAIAVMALWPLHQQFGLKRILCSTYQAASGAGQEGMEELRTGMEHYLIGREYKNEVFAHPLPFNVIPHIDKFQANGYTKEEMKVAWETKKIFDYPHMDIACTAVRVPTLRAHAESITIETYLDIEPSAALFALRKAPGVEVRDSGEENLYPMPLTATGKYDVEVGRVRQSLVFGSKGLELFVCGDQLLRGAASNAVFIAEAVL 397
            DR FPV    L LFASERSAGK ++T YG K I LFSVE A++CD VFLAVSG FA ++A +I++  G  VIDNSSAFR DDD+PL+VPEIN A    ++LIANPNCTTAIA +ALWPLHQ FGLK+++ STYQAASGAGQ GM ELR G+   L G+    EVFAHPL FNVIPHID FQ NGYT+EEMKV WET+KIF  P++ ++CTAVR+P  R H+E+I IET   + P+AA  AL KA GV++ D    N+YPMP+ AT +YDVEVGR+R +L+FG  GLELFV GDQLL+GAA NAV IAEA +
Sbjct:   27 DRNFPV--GELTLFASERSAGKTMETKYGTKTIALFSVEEAKQCDIVFLAVSGDFAKEFAPQIAK--GALVIDNSSAFRMDDDVPLIVPEINGALAKTAKLIANPNCTTAIAAVALWPLHQAFGLKKVIVSTYQAASGAGQPGMNELREGLAAGLEGKPVSAEVFAHPLAFNVIPHIDSFQENGYTREEMKVTWETRKIFGEPNLAVSCTAVRIPIERTHSEAIVIETEKVVTPAAAREALAKAKGVKLVDDPANNVYPMPINATKQYDVEVGRIRTNLIFGDHGLELFVAGDQLLKGAALNAVQIAEAYI 333          

BLAST of NO18G01840 vs. NCBI_GenBank
Match: XP_008609066.1 (aspartate-semialdehyde dehydrogenase [Saprolegnia diclina VS20] >EQC37546.1 aspartate-semialdehyde dehydrogenase [Saprolegnia diclina VS20])

HSP 1 Score: 393.7 bits (1010), Expect = 7.500e-106
Identity = 197/308 (63.96%), Postives = 241/308 (78.25%), Query Frame = 0

Query:   86 DRRFPVTLDTLRLFASERSAGKKIKTPYGDKKIELFSVESARECDFVFLAVSGTFALQYAKKISEKGGPYVIDNSSAFRYDDDIPLVVPEINSAAIGASRLIANPNCTTAIAVMALWPLHQQFGLKRILCSTYQAASGAGQEGMEELRTGMEHYLIGREYKNEVFAHPLPFNVIPHIDKFQANGYTKEEMKVAWETKKIFDYPHMDIACTAVRVPTLRAHAESITIETYLDIEPSAALFALRKAPGVEVRDSGEENLYPMPLTATGKYDVEVGRVRQSLVFGSKGLELFVCGDQLLRGAASNAVFIAE 394
            DR+F VT  ++ LFAS RSAGK ++TP+G  +++LF+VE+AR  D+VFLAVSG+FAL++A+ I+  GG  VIDNSSAFR    +PLV+PEIN A+IG+ RLIANPNCTTAIAVMALWPLH  +G+++++ STYQAASGAG EGM+EL    +  L G    ++VFAHPL FNVIPHID FQ NGYTKEEMKV WET KIFD  +M I+CTAVR+P LRAH+E+ITI+T   I P+ AL  LR+A GV+V D    N YPMPL AT KY++EVGRVRQS+VF   GL+LFV GDQLLRGAA NAV IAE
Sbjct:   23 DRKFAVT--SVHLFASARSAGKVVETPFGPSEVQLFTVEAARAMDYVFLAVSGSFALEHAEAIAAPGGAVVIDNSSAFRMQPAVPLVIPEINGASIGSKRLIANPNCTTAIAVMALWPLHCHYGIEKMIVSTYQAASGAGVEGMDELLNTTKDALAGASPSHKVFAHPLAFNVIPHIDVFQPNGYTKEEMKVTWETNKIFDEANMKISCTAVRIPILRAHSEAITIQTKRPIAPADALALLRQADGVDVVDEPLANKYPMPLNATSKYNIEVGRVRQSIVFEEHGLDLFVSGDQLLRGAALNAVLIAE 328          

BLAST of NO18G01840 vs. NCBI_GenBank
Match: XP_002178397.1 (predicted protein [Phaeodactylum tricornutum CCAP 1055/1] >EEC50062.1 predicted protein [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 393.3 bits (1009), Expect = 9.800e-106
Identity = 195/312 (62.50%), Postives = 244/312 (78.21%), Query Frame = 0

Query:   87 RRFPVTLDTLRLFASERSAGKKIKTPYGDKKIELFSVESARECDFVFLAVSGTFALQYAKKISEKGGPYV-IDNSSAFRYDDDIPLVVPEINSAAIGASRLIANPNCTTAIAVMALWPLHQQFGLKRILCSTYQAASGAGQEGMEELRTGMEHYLIGREYKNEVFAHPLPFNVIPHIDKFQANGYTKEEMKVAWETKKIFDYP-HMDIACTAVRVPTLRAHAESITIETYLDIEPSAALFALRKAPGVEVRDSGEENLYPMPLTATGKYDVEVGRVRQSLVFGSKGLELFVCGDQLLRGAASNAVFIAEAVL 397
            R FPV  + LR+F S RSAG  + T YGD  +ELF V +A++CD VFLAVSG F+L++A+ +++     V IDNSSAFRY+ D+PLVVPEIN A    S+LIANPNCTTAIA+MALWPLHQ FGLK+++ STYQAASGAGQ GM+EL  G + YL G   +N +FAHPLPFNVIP IDKFQ+NGYTKEEMKV WET+KI   P    ++CTAVR+PT+RAH+ESI +ET   ++ +AA  AL +APGV++ D+ E+  YPMP TATG+YDVEVGR+R+S+V+G  GLELFVCGDQLLRGAA NAV +AE ++
Sbjct:   25 RDFPV--ERLRIFGSARSAGSTVATKYGDVTVELFDVAAAKQCDVVFLAVSGDFSLEHARHLTQGDDATVCIDNSSAFRYEADVPLVVPEINGAETKKSKLIANPNCTTAIALMALWPLHQLFGLKKVIMSTYQAASGAGQPGMDELTQGTQAYLNGEAVENNIFAHPLPFNVIPQIDKFQSNGYTKEEMKVTWETRKICGLPDDFPLSCTAVRIPTIRAHSESIVVETERPVDVAAARQALAQAPGVKLVDNTEQLEYPMPQTATGQYDVEVGRLRKSIVYGDYGLELFVCGDQLLRGAALNAVLVAEEMV 334          

BLAST of NO18G01840 vs. NCBI_GenBank
Match: WP_007091858.1 (MULTISPECIES: aspartate-semialdehyde dehydrogenase [Thalassospira] >KEO54670.1 aspartate-semialdehyde dehydrogenase [Thalassospira permensis NBRC 106175] >AJD50257.1 aspartate-semialdehyde dehydrogenase [Thalassospira xiamenensis M-5 = DSM 17429] >SIT27223.1 aspartate semialdehyde dehydrogenase [Thalassospira xiamenensis M-5 = DSM 17429])

HSP 1 Score: 392.9 bits (1008), Expect = 1.300e-105
Identity = 197/311 (63.34%), Postives = 236/311 (75.88%), Query Frame = 0

Query:   86 DRRFPVTLDTLRLFASERSAGKKIKTPYGDKKIELFSVESARECDFVFLAVSGTFALQYAKKISEKGGPYVIDNSSAFRYDDDIPLVVPEINSAAIGASRLIANPNCTTAIAVMALWPLHQQFGLKRILCSTYQAASGAGQEGMEELRTGMEHYLIGREYKNEVFAHPLPFNVIPHIDKFQANGYTKEEMKVAWETKKIFDYPHMDIACTAVRVPTLRAHAESITIETYLDIEPSAALFALRKAPGVEVRDSGEENLYPMPLTATGKYDVEVGRVRQSLVFGSKGLELFVCGDQLLRGAASNAVFIAEAVL 397
            DR FPV    L LFASERSAGK ++T YG K I LFSVE A++CD VFLAVSG FA ++A +I+E  G  VIDNSSAFR +DD+PL+VPEIN      ++L+ANPNCTTAIA +ALWPLH+ FGLK++  STYQAASGAGQ GM ELR G+   L GR    EVFAHPL FNVIPHID FQ NGYT+EEMKV WET+KIF  P++ ++CTAVR+PT R H+ESI IET   + P+AA  AL  A GV++ D    N+YPMP+ AT +YDVEVGR+R +L+FG  GLELFV GDQLL+GAA NAV IAEA +
Sbjct:   27 DRNFPV--GELTLFASERSAGKTVETKYGTKTIALFSVEEAKQCDIVFLAVSGDFAKEFAPQIAE--GALVIDNSSAFRLNDDVPLIVPEINGDLAKTAKLVANPNCTTAIAAVALWPLHKAFGLKKVFVSTYQAASGAGQPGMNELREGLAAGLEGRPVPAEVFAHPLAFNVIPHIDSFQENGYTREEMKVTWETRKIFGTPNLPVSCTAVRIPTERTHSESIVIETEKAVTPAAAREALANAKGVKLVDDPANNVYPMPINATKQYDVEVGRIRSNLIFGDHGLELFVAGDQLLKGAALNAVQIAEAYI 333