NO17G02100, NO17G02100 (gene) Nannochloropsis oceanica

BLAST of NO17G02100 vs. NCBI_GenBank
Match: EWM24202.1 (protein kinase domain containing protein [Nannochloropsis gaditana])

HSP 1 Score: 454.9 bits (1169), Expect = 4.000e-124
Identity = 316/522 (60.54%), Postives = 357/522 (68.39%), Query Frame = 0

Query:   84 MELCPYGDLEQKIKRHQHRKEYIDEREIWVYSVNLLEGLAALHAKGVCHRDLKPANCLIDKHGCIKIADMNISKVSKGGNGGMMQTQVGTPYFICPEIYLKKPYSSMSDMWSLGGVLYNLAALHPPFLADNIQNLRKVVVRGAYDPLPNVYGTSLAALIGRLLQINPSDRPEAKDILKDPLVERHKYLLLHPLPVGSEEAEGEMLPTIRVASDKGGVKTITLPGPAYEEE--------GGNEXXXXXXXXXXXXXXXFVVYSPRAKXXXXXXXXXXXXXXXXXXXXXXXXXXXXGDGMNVGAHPLKRSAQPRTNPGXXXXXXXXXXXXXXXXXXXXXPRPRSXPSVSIPASXXXXXXXXXXXXXXXXXXXXXXXXTPFPPSAAGSPARTSSPLTSLLPALQDLGRDLARHLPSRASLSPKELLRQQQEKEKARLAAANFGGGAIFVDTGDDSHEALPDSSAGAASATGSGGPAEGKSKRSSIRKVFDGESIPAFPT------AEMKNFFSGGKLPLLPSKGK 592
            MELCPYGDLEQKIKRH+HRK+YIDEREIWVY+VNLLEGLAALH+KGV HRDLKPANCLID  GC+KIADMNISKVSKGGN   MQTQVGTPYFICPEIYLK+PY+S SD+WSLGGVLYNLAAL PPFLADNIQ LR+VV+RG++DPLP+V+G SL  LIG+LLQINPSDRPEAK+ILKDPLVERHKYLLLHPLP G EEAEGEMLPTIRVASDK G KTI LPGPAYEEE        G                  FVV SPRAK XXXXXXXXXXXXXXXXXXXXXXXXXXX                      XXXXXXXXXXXXXXXX       P   PS   P                 XXXXXXXXX                      PALQD+GRD+ RHLP R    P   + ++++   A +AA  +GGGA+F+DTG+  ++ + D +   A++T  G    GK+KRSSIR+VFDGE +P+FPT      AE+KNFF+       P  G+
Sbjct:    1 MELCPYGDLEQKIKRHKHRKQYIDEREIWVYAVNLLEGLAALHSKGVVHRDLKPANCLIDSQGCVKIADMNISKVSKGGN---MQTQVGTPYFICPEIYLKRPYTSTSDIWSLGGVLYNLAALRPPFLADNIQKLRRVVIRGSFDPLPSVFGQSLTTLIGQLLQINPSDRPEAKEILKDPLVERHKYLLLHPLPAGQEEAEGEMLPTIRVASDKEGTKTIRLPGPAYEEEETGAGTRAGKERGARDRPPSPRSPTSVFVVSSPRAKDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGRL---SPLKTPS---PTQILPSFPFASPKVGGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPALQDMGRDMVRHLPVR---PPGMKVSKEEKARLAAIAAGEYGGGAVFIDTGE--YDDVGDVAGSPATSTSVG----GKAKRSSIRRVFDGEGLPSFPTLEAFPVAEVKNFFANANGRRTPPPGE 504          

BLAST of NO17G02100 vs. NCBI_GenBank
Match: XP_005856094.1 (protein kinase 2-like protein [Nannochloropsis gaditana CCMP526] >EKU20269.1 protein kinase 2-like protein [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 316.6 bits (810), Expect = 1.700e-82
Identity = 159/324 (49.07%), Postives = 220/324 (67.90%), Query Frame = 0

Query:    1 MGLDDFDVIQTLGKGAFARVDKVKRKVDAKIYALKRVDISNIPPKDLEDSLNEIRILASFKHPRLIRWYETFVESELQELCIVMELCPYGDLEQKIKRHQHRKEYIDEREIWVYSVNLLEGLAALHAKGVCHRDLKPANCLIDKHGCIKIADMNISKVSKGGNGGMMQTQVGTPYFICPEIYLKKPYSSMSDMWSLGGVLYNLAALHPPFLADNIQNLRKVVVRGAYDPLPNVYGTSLAALIGRLLQINPSDRPEAKDILKDPLVERHKYLLLHPL---------PVGSEEAEGEMLPTIRVASDKG-GVKTITLPGPAYEEEG 315
            MG++ F+++++LG+GAFA V KV R VD K YALK+VD+S++  K+L  +LNEIR+LASF HPR++R +ETF++     LCIVME C +GDL  KIKR+  R+EYIDER IWVY + +LEGL ALH + V HRDLKPANC + + G IKI DMN+SKV K GN    +TQ+GTPY++ PEI+ ++PY+  +D+WSLG ++Y L AL PPFL +N+  L+  V+ G ++P+P+VY   L ++I R+L     DRP A + L  P V   K L+ + L          VG   AE  ++PTI + S +  GVK   LPGP+Y  +G
Sbjct:    1 MGIEQFEILKSLGEGAFASVHKVTRLVDGKTYALKKVDVSSLDDKELLSALNEIRLLASFGHPRIVRLHETFMDG--NNLCIVMEYCGWGDLAMKIKRYVKRREYIDERVIWVYMIQILEGLKALHERNVLHRDLKPANCFLAEDGSIKIGDMNVSKVMKDGNA---KTQIGTPYYMSPEIWARRPYNHATDIWSLGCLIYELCALRPPFLGNNMSELKTAVLGGNFNPVPSVYSKDLGSVIARMLLAAARDRPSAAEALAYPEVNARKCLVKNVLREEELYTKGGVGGYGAEDALMPTIHIGSLRELGVK---LPGPSYPSDG 316          

BLAST of NO17G02100 vs. NCBI_GenBank
Match: EWM23138.1 (protein kinase [Nannochloropsis gaditana])

HSP 1 Score: 313.9 bits (803), Expect = 1.100e-81
Identity = 157/324 (48.46%), Postives = 219/324 (67.59%), Query Frame = 0

Query:    1 MGLDDFDVIQTLGKGAFARVDKVKRKVDAKIYALKRVDISNIPPKDLEDSLNEIRILASFKHPRLIRWYETFVESELQELCIVMELCPYGDLEQKIKRHQHRKEYIDEREIWVYSVNLLEGLAALHAKGVCHRDLKPANCLIDKHGCIKIADMNISKVSKGGNGGMMQTQVGTPYFICPEIYLKKPYSSMSDMWSLGGVLYNLAALHPPFLADNIQNLRKVVVRGAYDPLPNVYGTSLAALIGRLLQINPSDRPEAKDILKDPLVERHKYLLLHPL---------PVGSEEAEGEMLPTIRVASDKG-GVKTITLPGPAYEEEG 315
            MG++ F+++++LG+GAFA V KV R VD K YALK+VD+S++  K+L  +LNEIR+LASF HPR++R +ETF++     LCIVME C +GDL  KIKR+  R+EYIDER IWVY + +LEGL ALH + V HRDLKPANC + + G IKI DMN+SKV K GN    +TQ+GTPY++ PEI+ ++PY+  +D+WSLG ++Y L AL PPFL +N+  L+  V+ G ++P+P+VY   L ++I R+L     DRP A + L  P V   K L+ + L           G   AE  ++PTI + S +  G+K   LPGP+Y  +G
Sbjct:    1 MGIEQFEILKSLGEGAFASVHKVTRLVDGKTYALKKVDVSSLDDKELLSALNEIRLLASFGHPRIVRLHETFMDG--NNLCIVMEYCGWGDLAMKIKRYVKRREYIDERVIWVYMIQILEGLKALHERNVLHRDLKPANCFLAEDGSIKIGDMNVSKVMKDGNA---KTQIGTPYYMSPEIWARRPYNHATDIWSLGCLIYELCALRPPFLGNNMSELKTAVLGGNFNPVPSVYSKDLGSVIARMLLAAARDRPSAAEALAYPEVNARKCLVKNVLREEELYTKGGKGGYGAEDALMPTIHIGSLRELGIK---LPGPSYPSDG 316          

BLAST of NO17G02100 vs. NCBI_GenBank
Match: OMJ87286.1 (hypothetical protein SteCoe_11057 [Stentor coeruleus])

HSP 1 Score: 267.7 bits (683), Expect = 9.100e-68
Identity = 134/311 (43.09%), Postives = 195/311 (62.70%), Query Frame = 0

Query:    1 MGLDDFDVIQTLGKGAFARVDKVKRKVDAKIYALKRVDISNIPPKDLEDSLNEIRILASFKHPRLIRWYETFVESELQELCIVMELCPYGDLEQKIKRHQHRKEYIDEREIWVYSVNLLEGLAALHAKGVCHRDLKPANCLIDKHGCIKIADMNISKVSKGGNGGMMQTQVGTPYFICPEIYLKKPYSSMSDMWSLGGVLYNLAALHPPFLADNIQNLRKVVVRGAYDPLPNVYGTSLAALIGRLLQINPSDRPEAKDILKDPLVERHKYLLLHPLPVGSEEAEGEMLPTIRVASDKGGVKTITLPGPAYE 312
            M + DF+++  LG GAF+ V KV RK D++ YALK+V +  + PK+ E++LNE+RILAS  HP +I + E F++     LCIVMEL   GDL +KI  H+  + +  E E+W   V++ +GL  LH   + HRDLK AN  + K G +K+ D+N+SKV+K    G+  TQ GTPY+  PE++  +PY+S SD+WSLG V+Y + AL PPF A ++Q L K ++RG Y  +P+ Y T L+ +I  LLQ+NP+ RP    IL+ P V+RH  +L  P P  +     ++L TI+       +K   LPGP Y+
Sbjct:    1 MSISDFEILSKLGDGAFSSVFKVVRKSDSQTYALKKVKLGTLKPKEKENALNEVRILASVYHPNIIAYKEVFIDEPTNTLCIVMELAEGGDLLKKISNHKAARTFFPENEVWQAIVHVTQGLKVLHDTKILHRDLKCANIFVSKEGVMKLGDLNVSKVNK---RGLAYTQTGTPYYASPEVWKDQPYNSSSDIWSLGCVIYEMVALVPPFTATDMQGLYKKIIRGIYPNIPSQYSTDLSNVIRTLLQVNPTLRPSCDKILEMPPVQRHINVLAMPNPQAA-----DLLGTIKFEPGMKNLKQ-KLPGPNYD 302          

BLAST of NO17G02100 vs. NCBI_GenBank
Match: OMJ86601.1 (hypothetical protein SteCoe_11813 [Stentor coeruleus])

HSP 1 Score: 261.9 bits (668), Expect = 5.000e-66
Identity = 131/315 (41.59%), Postives = 194/315 (61.59%), Query Frame = 0

Query:    1 MGLDDFDVIQTLGKGAFARVDKVKRKVDAKIYALKRVDISNIPPKDLEDSLNEIRILASFKHPRLIRWYETFVESELQELCIVMELCPYGDLEQKIKRHQHRKEYIDEREIWVYSVNLLEGLAALHAKGVCHRDLKPANCLIDKHGCIKIADMNISKVSKGGNGGMMQTQVGTPYFICPEIYLKKPYSSMSDMWSLGGVLYNLAALHPPFLADNIQNLRKVVVRGAYDPLPNVYGTSLAALIGRLLQINPSDRPEAKDILK-DPLVERHKYLLLHPLPVGSEEAEGEMLPTIRVASDKGGVKTITLPGPAYEEEG 315
            M +  F+++  LG+GAF+ V +V+R+ D  IYALK+V +SN+  K+ E++LNE+RILAS  H  +I + E F++     LCIVME    GDL   I +H+       E+EIW   + LL GL ALH   + HRDLK AN  +  +G  KI D+N+SKV+K    G+  TQ GTPY+  PE++  +PY+S SD+WSLG V+Y +  L+PPF+A ++QNL + V++G Y  +PN+Y T L+ +I  ++Q+NPS+RP  + IL+  P++    Y       V S +A  E+L TI+       + T  LP   YE+ G
Sbjct:    1 MSVSQFEILSKLGEGAFSNVFRVRRRSDGNIYALKKVKLSNLGAKERENALNEVRILASISHQNVIAYKEAFIDEGTSTLCIVMEYADSGDLLNLIDKHKRESSRFTEKEIWDILIQLLRGLKALHGLNILHRDLKCANVFLSSNGIAKIGDLNVSKVNK---QGLAYTQTGTPYYASPEVWRDQPYNSSSDIWSLGCVIYEMTTLNPPFMATDMQNLYRKVIKGLYPEIPNIYSTDLSNVIRVMIQVNPSNRPSCEKILEMAPVIRNMNY-----KKVDSVDAPAELLQTIKFVPSL-RMLTNRLPAANYEKRG 306          

BLAST of NO17G02100 vs. NCBI_GenBank
Match: OMJ77922.1 (hypothetical protein SteCoe_22397 [Stentor coeruleus])

HSP 1 Score: 259.6 bits (662), Expect = 2.500e-65
Identity = 135/314 (42.99%), Postives = 193/314 (61.46%), Query Frame = 0

Query:    1 MGLDDFDVIQTLGKGAFARVDKVKRKVDAKIYALKRVDISNIPPKDLEDSLNEIRILASFKHPRLIRWYETFVESELQELCIVMELCPYGDLEQKIKRHQHRKEYIDEREIWVYSVNLLEGLAALHAKGVCHRDLKPANCLIDKHGCIKIADMNISKVSKGGNGGMMQTQVGTPYFICPEIYLKKPYSSMSDMWSLGGVLYNLAALHPPFLADNIQNLRKVVVRGAYDPLPNVYGTSLAALIGRLLQINPSDRPEAKDILKDPLVERHKYLLLHPLPVGSEEAEGEMLPTIRVASDKGGVKTITLPGPAYEEEG 315
            M LD F++I+ LG+GAF+ V KV RK D + YALK+V +  +  K+ E++LNEIRI+ASF H  +I + ++F++ +   LCIVMEL   GDL +KI  H+ +     E EIW   + +  GL  LH   + HRDLK AN  + K G IK+ D+N+SKV+K    G+  TQ GTPY+  PE++  KPY+  SD+WSLG V+Y +AAL PPF+A ++Q L K +++G Y  +P++Y  SL  +I  LLQ+NP+ RP  + IL  P V+R  Y    P     +    ++L TI+       +KT  LP P YE  G
Sbjct:    9 MSLDSFEIIRKLGEGAFSCVYKVNRKSDGQCYALKKVKLGTLCIKEKENALNEIRIMASFCHSNIIAYKDSFIDEKSNTLCIVMELAEGGDLHKKINEHKKQGSNFSEYEIWSTLMQITYGLKVLHNANIIHRDLKCANIFVTKDGVIKLGDLNVSKVNK---LGLAYTQTGTPYYASPEVWRDKPYTYSSDIWSLGCVIYEMAALEPPFVASDMQALYKKIIKGEYPEIPHIYSDSLVRVIRHLLQVNPNLRPTCEQILNFPTVKR--YTSFSP---RKDSENPDLLKTIKFEPCIQNLKT-KLPSPNYENRG 313          

BLAST of NO17G02100 vs. NCBI_GenBank
Match: KRX07956.1 (Protein kinase-like domain [Pseudocohnilembus persalinus])

HSP 1 Score: 258.5 bits (659), Expect = 5.500e-65
Identity = 136/312 (43.59%), Postives = 199/312 (63.78%), Query Frame = 0

Query:    1 MGLDDFDVIQTLGKGAFARVDKVKRKVDAKIYALKRVDISNIPPKDLEDSLNEIRILASFKHPRLIRWYETFVESELQELCIVMELCPYGDLEQKIKRHQHRKEYIDEREIWVYSVNLLEGLAALHAKGVCHRDLKPANCLIDKHGCIKIADMNISKVSKGGNGGMMQTQVGTPYFICPEIYLKKPYSSMSDMWSLGGVLYNLAALHPPFLADNIQNLRKVVVRGAYDPLPNVYGTSLAALIGRLLQINPSDRPEAKDILKDPLVERH--KYLLLHPLPVGSEEAEGEMLPTIRVASDKGGVKTITLPGPAY 311
            M +++F VIQ LG GA++ V KVKR  D ++YALK+V + N+  K+  ++LNE+RILAS +HP +I + E F++ +   LCIVME C  GDL QKI ++Q + +   E+EI+   V +++GL  LH   + HRDLK AN  + K G +K+ DMN+SKV+K    G++ TQ GTPY+  PE++  +PY S SD+WSLG VLY +  L PPF A++++ L K V+RG Y  +P+ Y   L A+I +LLQ+NP  RP ++ IL+ P + RH  +    H   + SEE    +L TI+V  +   + T  LP P Y
Sbjct:    1 MSVNNFQVIQRLGDGAYSSVYKVKRHDDGEVYALKKVKLMNLSQKEKLNALNEVRILASIRHPNVIGYKEAFLDDKSNSLCIVMEYCDGGDLFQKICQYQKKNQLFPEKEIFSILVQIVKGLQELHQLKIFHRDLKSANVFLCKDGSVKLGDMNVSKVAK---KGLLYTQTGTPYYASPEVWKDQPYESKSDIWSLGCVLYEMTTLKPPFRAEDMEGLYKRVIRGQYPKIPSHYSADLGAVIKQLLQVNPHMRPSSEKILQLPGLVRHMRESNFEH---LESEEGNNNLLQTIKVPRNLHFL-TDRLPKPNY 305          

BLAST of NO17G02100 vs. NCBI_GenBank
Match: EJY69153.1 (Protein kinase domain containing protein (macronuclear) [Oxytricha trifallax])

HSP 1 Score: 255.4 bits (651), Expect = 4.600e-64
Identity = 132/306 (43.14%), Postives = 191/306 (62.42%), Query Frame = 0

Query:    5 DFDVIQTLGKGAFARVDKVKRKVDAKIYALKRVDISNIPPKDLEDSLNEIRILASFKHPRLIRWYETFVESELQELCIVMELCPYGDLEQKIKRHQHRKEYIDEREIWVYSVNLLEGLAALHAKGVCHRDLKPANCLIDKHGCIKIADMNISKVSKGGNGGMMQTQVGTPYFICPEIYLKKPYSSMSDMWSLGGVLYNLAALHPPFLADNIQNLRKVVVRGAYDPLPNVYGTSLAALIGRLLQINPSDRPEAKDILKDPLVERHKYLLLHPLPVGSEEAEGEMLPTIRVASDKGG----VKTITLP 307
            DF VI  LG+GA+++V KVKR  D +IYALK+V +  +  K+ E++LNE+RILAS +HP ++ + E F+E + + LCI+ME    GDL QKI  HQ      DE  IW Y ++++ GL ALH   + HRD+K AN  I+K+G  K+ D+N+SKV+K    G++ TQ GTPY+  PE++  +PY   SDMWSLG VLY +A L PPF A+++  L K V++G+Y  +P  Y + LA +I +L+ ++ S RP    IL +P+V++        L + SE  E E    I V+   GG    + TI LP
Sbjct:    7 DFTVINKLGEGAYSQVYKVKRLSDNQIYALKKVSLDPLSQKERENALNEVRILASIQHPNVVGYKEAFLEEQEKYLCIIMEYADDGDLYQKIIEHQRNSTLFDEDTIWRYFIHMVRGLKALHKLKIFHRDMKSANIFINKNGTAKLGDLNVSKVAK---KGLLYTQTGTPYYASPEVWQDQPYDQKSDMWSLGCVLYEIATLQPPFKANDMDGLFKKVLKGSYPQIPEQYSSDLAKIIKKLICVHASGRPSCDQILNNPVVKK----WCTKLGISSETDEIENEQQIVVSDGLGGNLALLSTIVLP 305          

BLAST of NO17G02100 vs. NCBI_GenBank
Match: CDW85297.1 (protein kinase domain containing protein [Stylonychia lemnae])

HSP 1 Score: 252.7 bits (644), Expect = 3.000e-63
Identity = 125/308 (40.58%), Postives = 186/308 (60.39%), Query Frame = 0

Query:    3 LDDFDVIQTLGKGAFARVDKVKRKVDAKIYALKRVDISNIPPKDLEDSLNEIRILASFKHPRLIRWYETFVESELQELCIVMELCPYGDLEQKIKRHQHRKEYIDEREIWVYSVNLLEGLAALHAKGVCHRDLKPANCLIDKHGCIKIADMNISKVSKGGNGGMMQTQVGTPYFICPEIYLKKPYSSMSDMWSLGGVLYNLAALHPPFLADNIQNLRKVVVRGAYDPLPNVYGTSLAALIGRLLQINPSDRPEAKDILKDPLVERHKYLLLHPLPVGSEEAEGEMLPTIRVASDKGGVKTITLPGPAY 311
            + +F V+Q LG+GA+++V KVKR  D   YA+K+V +  +  ++ ++++NE+RILAS +HP +I + E F E     LCI+ME    GDL ++I  H+ +     E+E W Y + ++ GL ALH   +CHRD+K AN  + K G +K+ D+N+SKV+K    GM+ TQ GTPY+  PE++  KPY   SD+WSLG VLY +  L+PPF A N+  L   V+RG YDP+P++Y   L  ++   LQ++P  RP  + IL  P +  H    L  + V  +     +L TI+V  D G + T  LP P Y
Sbjct:   13 ITNFHVMQKLGEGAYSQVFKVKRISDGAEYAMKKVKMIKLSERERQNAVNEVRILASIQHPNIIAYKEAFFEDSTSCLCIIMEYADGGDLLKQINNHKRKGTNFSEKESWYYFIQMVRGLKALHDLKICHRDIKCANLFLTKEGIVKLGDLNVSKVAK---KGMLHTQTGTPYYASPEVWKDKPYDHRSDIWSLGCVLYEMLTLNPPFRAPNMNGLYHKVLRGLYDPVPSMYTKDLQFMVRNCLQVSPVARPSCEKILAMPGLLNHLTGTLERIDVSIDVENDALLNTIKVPKDLGQI-TERLPKPQY 316          

BLAST of NO17G02100 vs. NCBI_GenBank
Match: OMJ88081.1 (hypothetical protein SteCoe_10066 [Stentor coeruleus])

HSP 1 Score: 252.7 bits (644), Expect = 3.000e-63
Identity = 129/313 (41.21%), Postives = 195/313 (62.30%), Query Frame = 0

Query:    1 MGLDDFDVIQTLGKGAFARVDKVKRKVDAKIYALKRVDISNIPPKDLEDSLNEIRILASFKHPRLIRWYETFVESELQELCIVMELCPYGDLEQKIKRHQHRKEYIDEREIWVYSVNLLEGLAALHAKGVCHRDLKPANCLIDKHGCIKIADMNISKVSKGGNGGMMQTQVGTPYFICPEIYLKKPYSSMSDMWSLGGVLYNLAALHPPFLADNIQNLRKVVVRGAYDPLPNVYGTSLAALIGRLLQINPSDRPEAKDILKDPLVERHKYLLLHPLPVGSEEAEGEMLPTIRVASDKGGVKTITLPGPAYEEE 314
            M + DF+++  LG+GAF+ V KV+RK D + YALK+V + ++  K+ E++LNE+RILAS  HP +I + E F++     LCI+MEL   GDL +KI   +  + +  E EI    V++ +GL  LH   + HRDLK AN  I + G +K+ D+N+SKV+K    G+  TQ GTPY+  PE++  +PY+S SD+WSLG V+Y + AL PPF A +++ L   ++RG Y  +P+ Y + L+++I  LLQ NPS RP    IL+ PLV+  KY+ +  LP      + ++L TI+       +K   LPGP Y+ +
Sbjct:    1 MSISDFEILSKLGEGAFSSVYKVRRKSDNQFYALKKVQLGSLKQKEKENALNEVRILASISHPNIIAYKEVFIDDSSNLLCIIMELAEGGDLLKKINDQKSSRSFFPENEILSTLVHVTQGLKILHDSKILHRDLKCANIFISREGVMKLGDLNVSKVNK---RGLAYTQTGTPYYASPEVWKDQPYNSSSDIWSLGCVIYEMVALTPPFTASDMKGLYTKIIRGIYPDIPSQYSSDLSSIIKSLLQTNPSLRPNCDKILQLPLVQ--KYINIQLLP---NLQKADLLSTIKFEPRMTNLKQ-KLPGPNYDNK 304