NO17G00670, NO17G00670 (gene) Nannochloropsis oceanica

Overview
NameNO17G00670
Unique NameNO17G00670
Typegene
OrganismNannochloropsis oceanica (N. oceanica IMET1)
Sequence length1123
Alignment locationchr17:235683..236805 +

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Properties
Property NameValue
DescriptionCytosine deaminase
Mutants
Expression

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
chr17genomechr17:235683..236805 +
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
PRJNA7699582024-08-13
GSE1786722023-12-15
PRJNA9336932023-10-26
PRJNA9434492023-07-19
GSE1499042020-10-10
NoIMET1_WT_HS2020-09-29
GSE1396152020-03-11
PRJNA2413822020-03-11
BLASTP analysis of N. oceanica IMET1 genes2019-07-11
InterPro analysis for N. oceanica IMET1 genes2019-07-11
GO annotation for IMET1v2 genes2019-07-10
PRJNA1821802019-04-25
Gene prediction for N. oceanica IMET12017-10-24
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0008251tRNA-specific adenosine deaminase activity
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0002100tRNA wobble adenosine to inosine editing
Vocabulary: INTERPRO
TermDefinition
IPR016193Cytidine_deaminase-like
IPR002125CMP_dCMP_Zn-bd
IPR028883tRNA_aden_deaminase
Homology
BLAST of NO17G00670 vs. NCBI_GenBank
Match: EWM21163.1 (cytosine deaminase [Nannochloropsis gaditana])

HSP 1 Score: 230.3 bits (586), Expect = 5.500e-57
Identity = 117/169 (69.23%), Postives = 132/169 (78.11%), Query Frame = 0
Query:   48 MATSSPPPS---HIDHDAIYLQQALIEAQQGLEEGGIPIGAVLVLREAGGTRRVLGKGHNRRVQKGSAILHGEIDCLENAGRLPASVYKMCTMYTTLSPCSMCSGAILLFGIPRVVVGENKTFMGAEDYLSAQGVEVIVKQDEGCVQIMTEFIKENAELWEEDSGQSKE 214
            MAT  P P+     + D IYLQQAL EA+ GL EGGIPIGAVLV     G R VLG GHN+RVQK S +LHGE+DCLENAGR PASVY+ CTMYTTLSPCSMCSGAILLFGIPRVVVGEN+TF+GAE++L A GVEVIV QD  CV IM EF++E+  LWEED G+  E
Sbjct:    1 MATQKPQPATREKENEDDIYLQQALEEAKVGLNEGGIPIGAVLVHEGTDGVRHVLGMGHNQRVQKKSPVLHGEMDCLENAGRCPASVYRKCTMYTTLSPCSMCSGAILLFGIPRVVVGENQTFLGAEEHLRANGVEVIVVQDASCVAIMKEFVREHKVLWEEDIGEPSE 169          
BLAST of NO17G00670 vs. NCBI_GenBank
Match: OGU67358.1 (tRNA-specific adenosine deaminase [Ignavibacteria bacterium GWC2_38_9] >OGV07598.1 tRNA-specific adenosine deaminase [Ignavibacteria bacterium RIFOXYB2_FULL_37_11] >OGV10760.1 tRNA-specific adenosine deaminase [Ignavibacteria bacterium RIFOXYA2_FULL_38_8] >OGV12601.1 tRNA-specific adenosine deaminase [Ignavibacteria bacterium RIFOXYC2_FULL_38_25] >OGV26859.1 tRNA-specific adenosine deaminase [Ignavibacteria bacterium RIFOXYC12_FULL_38_8] >OGV78866.1 tRNA-specific adenosine deaminase [Melioribacter sp. GWF2_38_21])

HSP 1 Score: 198.4 bits (503), Expect = 2.300e-47
Identity = 93/146 (63.70%), Postives = 117/146 (80.14%), Query Frame = 0
Query:   64 YLQQALIEAQQGLEEGGIPIGAVLVLREAGGTRRVLGKGHNRRVQKGSAILHGEIDCLENAGRLPASVYKMCTMYTTLSPCSMCSGAILLFGIPRVVVGENKTFMGAEDYLSAQGVEVIVKQDEGCVQIMTEFIKENAELWEEDSG 210
            ++Q A+ EAQ+GLEEGGIPIG+V+V +      ++LG+GHNRRVQKGS ILHGE+D  ENAGR PASVYK C +YTTLSPC MCSGAI+L+GIP+V++GENKTFMG E++L ++GVEV V QDE C+ +MT FI +   LW ED G
Sbjct:    4 FMQAAIEEAQKGLEEGGIPIGSVIVHK-----GKILGRGHNRRVQKGSVILHGEMDAFENAGRQPASVYKECVLYTTLSPCPMCSGAIILYGIPKVIIGENKTFMGEEEHLKSRGVEVEVLQDETCINMMTNFIADKPTLWSEDIG 144          
BLAST of NO17G00670 vs. NCBI_GenBank
Match: OLC08886.1 (tRNA-specific adenosine deaminase [Gemmatimonadetes bacterium 13_1_40CM_66_11] >OLD15352.1 tRNA-specific adenosine deaminase [Acidobacteria bacterium 13_1_40CM_3_65_5])

HSP 1 Score: 198.4 bits (503), Expect = 2.300e-47
Identity = 93/146 (63.70%), Postives = 119/146 (81.51%), Query Frame = 0
Query:   64 YLQQALIEAQQGLEEGGIPIGAVLVLREAGGTRRVLGKGHNRRVQKGSAILHGEIDCLENAGRLPASVYKMCTMYTTLSPCSMCSGAILLFGIPRVVVGENKTFMGAEDYLSAQGVEVIVKQDEGCVQIMTEFIKENAELWEEDSG 210
            +L  A+ EA+QGL EGGIPIG+V+V R      +++G+GHNRRVQKGSA+LHGE+D LENAGR PASVY+  T+YTTLSPC+MCSGAILL+GIPRV++GENKTF+G E  LS +GV + V+QD  C+++MTEFI+ + ELW ED G
Sbjct:    6 FLAAAIAEAEQGLREGGIPIGSVIVSR-----GKIIGRGHNRRVQKGSAVLHGEVDALENAGRQPASVYRGATLYTTLSPCAMCSGAILLYGIPRVIIGENKTFVGEEGLLSGRGVSLEVRQDPTCIRMMTEFIRTHPELWNEDIG 146          
BLAST of NO17G00670 vs. NCBI_GenBank
Match: WP_017937909.1 (nucleoside deaminase [Pseudomonas thermotolerans])

HSP 1 Score: 196.4 bits (498), Expect = 8.800e-47
Identity = 96/147 (65.31%), Postives = 118/147 (80.27%), Query Frame = 0
Query:   64 YLQQALIEAQQGLEEGGIPIGAVLVLREAGGTRRVLGKGHNRRVQKGSAILHGEIDCLENAGRLPASVYKMCTMYTTLSPCSMCSGAILLFGIPRVVVGENKTFMGAEDYLSAQGVEVIVKQDEGCVQIMTEFIKENAELWEEDSGQ 211
            ++Q A+ EA+QGLEEGGIPIG+VLV R      R++G+GHNRRVQKGSA LHGE+D LENAGRLPASVY+   +YTTLSPC MCSGAILL+GIPRVVVGEN+TFMG E+ L A+GV+V V QDE C+++M  FI+    +W ED G+
Sbjct:    4 FMQAAIDEARQGLEEGGIPIGSVLVHR-----GRIIGRGHNRRVQKGSATLHGEMDALENAGRLPASVYRDAVLYTTLSPCPMCSGAILLYGIPRVVVGENRTFMGEEELLRARGVQVEVLQDERCIELMRTFIERYPAVWNEDIGE 145          
BLAST of NO17G00670 vs. NCBI_GenBank
Match: WP_045225507.1 (nucleoside deaminase [Methyloterricola oryzae])

HSP 1 Score: 196.4 bits (498), Expect = 8.800e-47
Identity = 96/146 (65.75%), Postives = 119/146 (81.51%), Query Frame = 0
Query:   64 YLQQALIEAQQGLEEGGIPIGAVLVLREAGGTRRVLGKGHNRRVQKGSAILHGEIDCLENAGRLPASVYKMCTMYTTLSPCSMCSGAILLFGIPRVVVGENKTFMGAEDYLSAQGVEVIVKQDEGCVQIMTEFIKENAELWEEDSG 210
            +L+ A+ EA++GL EGGIPIG+V+V    GG  R++G+GHNRRVQ GSAILHGE+D LENAGR PASVY+ CT+YTTLSPC+MCSGAILL+GIPRVV+GEN+TFMG ED L A+GV + V  DE CV++M +FI  + ELW ED G
Sbjct:    4 FLRAAIEEAEKGLAEGGIPIGSVIV---HGG--RIIGRGHNRRVQSGSAILHGEMDALENAGRQPASVYRECTLYTTLSPCAMCSGAILLYGIPRVVIGENRTFMGEEDLLRARGVSLEVVDDETCVRLMRDFIAAHGELWNEDIG 144          
BLAST of NO17G00670 vs. NCBI_GenBank
Match: OYX23869.1 (tRNA-specific adenosine deaminase [Algoriphagus sp. 32-45-6])

HSP 1 Score: 195.7 bits (496), Expect = 1.500e-46
Identity = 93/147 (63.27%), Postives = 117/147 (79.59%), Query Frame = 0
Query:   64 YLQQALIEAQQGLEEGGIPIGAVLVLREAGGTRRVLGKGHNRRVQKGSAILHGEIDCLENAGRLPASVYKMCTMYTTLSPCSMCSGAILLFGIPRVVVGENKTFMGAEDYLSAQGVEVIVKQDEGCVQIMTEFIKENAELWEEDSGQ 211
            +LQ A+ EAQ GL+EGGIPIG+VLV R      ++LG+GHN+RVQ+ S  LHGE+D LENAGR PASVY+ CT+YTTLSPC MC+GAILL+GIP+VV+GEN+TF+G+E+ L   GVEVIVK D  C+Q+M EFI +N  LW ED G+
Sbjct:    4 FLQAAIEEAQTGLQEGGIPIGSVLVYR-----GQILGRGHNKRVQQKSVTLHGEMDALENAGRQPASVYRECTLYTTLSPCPMCTGAILLYGIPKVVIGENQTFLGSEELLKENGVEVIVKNDPTCIQLMEEFIAKNPALWNEDIGE 145          
BLAST of NO17G00670 vs. NCBI_GenBank
Match: WP_102653363.1 (nucleoside deaminase [Halomonas endophytica] >PMR75349.1 tRNA-specific adenosine deaminase [Halomonas endophytica])

HSP 1 Score: 195.3 bits (495), Expect = 2.000e-46
Identity = 95/147 (64.63%), Postives = 117/147 (79.59%), Query Frame = 0
Query:   64 YLQQALIEAQQGLEEGGIPIGAVLVLREAGGTRRVLGKGHNRRVQKGSAILHGEIDCLENAGRLPASVYKMCTMYTTLSPCSMCSGAILLFGIPRVVVGENKTFMGAEDYLSAQGVEVIVKQDEGCVQIMTEFIKENAELWEEDSGQ 211
            Y+Q AL EA+QGL EGGIPIG+VLV R      R+LG+GHNRRVQ GS +LHGE+D LENAGR  ASVY+ C +YTTLSPC MCSGAILL+GIP+VVVGEN+TFMG E+ L ++GVEV + Q E C+++MT FI+ + ELW ED G+
Sbjct:    4 YMQAALEEARQGLTEGGIPIGSVLVHR-----GRILGRGHNRRVQSGSTVLHGEMDALENAGRQAASVYRDCVLYTTLSPCPMCSGAILLYGIPKVVVGENRTFMGEEELLRSRGVEVEIIQSEECIELMTGFIEASPELWNEDIGE 145          
BLAST of NO17G00670 vs. NCBI_GenBank
Match: WP_107037200.1 (nucleoside deaminase [Brumimicrobium mesophilum])

HSP 1 Score: 195.3 bits (495), Expect = 2.000e-46
Identity = 95/146 (65.07%), Postives = 114/146 (78.08%), Query Frame = 0
Query:   64 YLQQALIEAQQGLEEGGIPIGAVLVLREAGGTRRVLGKGHNRRVQKGSAILHGEIDCLENAGRLPASVYKMCTMYTTLSPCSMCSGAILLFGIPRVVVGENKTFMGAEDYLSAQGVEVIVKQDEGCVQIMTEFIKENAELWEEDSG 210
            +  +A+ EA+ G+ EGGIPIG+VLV        +VLG+GHN+RVQKGS ILHGE+D LENAGRLPASVYK C +YTTLSPC MCSG ILL+GIP+VV+GENKTF+GAE +L   GVEVIV  D+ C ++M EFI EN ELW ED G
Sbjct:    4 FFLEAVKEAEIGIAEGGIPIGSVLV-----HNGKVLGRGHNKRVQKGSVILHGEMDALENAGRLPASVYKECVLYTTLSPCPMCSGTILLYGIPKVVIGENKTFLGAEKHLKENGVEVIVLDDKRCKELMDEFIAENPELWNEDIG 144          
BLAST of NO17G00670 vs. NCBI_GenBank
Match: WP_026903822.1 (nucleoside deaminase [Pedobacter glucosidilyticus])

HSP 1 Score: 194.5 bits (493), Expect = 3.300e-46
Identity = 91/146 (62.33%), Postives = 115/146 (78.77%), Query Frame = 0
Query:   64 YLQQALIEAQQGLEEGGIPIGAVLVLREAGGTRRVLGKGHNRRVQKGSAILHGEIDCLENAGRLPASVYKMCTMYTTLSPCSMCSGAILLFGIPRVVVGENKTFMGAEDYLSAQGVEVIVKQDEGCVQIMTEFIKENAELWEEDSG 210
            +++ A  EA++G EEGGIPIG+VLV        +++G+GHN+RVQKGS ILHGE+D LENAGRLPA +Y+ CT+YTTLSPC MCSG ILL+GIPRVV+ ENKTFMG E+ LS++GV+V+V   EGC Q+M +FIKE   LW ED G
Sbjct:    4 FMEAAFEEAKKGYEEGGIPIGSVLV-----HDGKIIGRGHNKRVQKGSVILHGEMDALENAGRLPAKIYQECTLYTTLSPCPMCSGTILLYGIPRVVIAENKTFMGEEELLSSRGVKVLVTHHEGCYQLMQQFIKEKPNLWNEDIG 144          
BLAST of NO17G00670 vs. NCBI_GenBank
Match: PKL82461.1 (tRNA-specific adenosine deaminase [Ignavibacteriae bacterium HGW-Ignavibacteriae-3])

HSP 1 Score: 194.5 bits (493), Expect = 3.300e-46
Identity = 94/146 (64.38%), Postives = 116/146 (79.45%), Query Frame = 0
Query:   64 YLQQALIEAQQGLEEGGIPIGAVLVLREAGGTRRVLGKGHNRRVQKGSAILHGEIDCLENAGRLPASVYKMCTMYTTLSPCSMCSGAILLFGIPRVVVGENKTFMGAEDYLSAQGVEVIVKQDEGCVQIMTEFIKENAELWEEDSG 210
            ++Q A+ EAQ G  EGGIPIG+VLV      + +++G+GHNRRVQKGSAILHGE+D LENAGRLPASVYK  T+YTTLSPC MCSGAILL+GIP+V+VGEN TFMG E++L ++GV V V  DE C+ +MT+FI+E   LW ED G
Sbjct:    4 FMQAAIEEAQLGFNEGGIPIGSVLVY-----SGKIIGRGHNRRVQKGSAILHGEMDALENAGRLPASVYKESTLYTTLSPCPMCSGAILLYGIPKVIVGENITFMGEEEHLKSRGVSVEVLDDETCINLMTKFIEEKPALWNEDIG 144          
The following BLAST results are available for this feature:
BLAST of NO17G00670 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes)
Total hits: 20
Match NameE-valueIdentityDescription
EWM21163.15.500e-5769.23cytosine deaminase [Nannochloropsis gaditana][more]
OGU67358.12.300e-4763.70tRNA-specific adenosine deaminase [Ignavibacteria ... [more]
OLC08886.12.300e-4763.70tRNA-specific adenosine deaminase [Gemmatimonadete... [more]
WP_017937909.18.800e-4765.31nucleoside deaminase [Pseudomonas thermotolerans][more]
WP_045225507.18.800e-4765.75nucleoside deaminase [Methyloterricola oryzae][more]
OYX23869.11.500e-4663.27tRNA-specific adenosine deaminase [Algoriphagus sp... [more]
WP_102653363.12.000e-4664.63nucleoside deaminase [Halomonas endophytica] >PMR7... [more]
WP_107037200.12.000e-4665.07nucleoside deaminase [Brumimicrobium mesophilum][more]
WP_026903822.13.300e-4662.33nucleoside deaminase [Pedobacter glucosidilyticus][more]
PKL82461.13.300e-4664.38tRNA-specific adenosine deaminase [Ignavibacteriae... [more]

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Relationships

This gene is member of the following syntenic_region feature(s):

Feature NameUnique NameSpeciesType
nonsL158nonsL158Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ncniR035ncniR035Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ngnoR084ngnoR084Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region


This gene is orthologous to the following gene feature(s):

Feature NameUnique NameSpeciesType
NSK004787NSK004787Nannochloropsis salina (N. salina CCMP1776)gene


The following polypeptide feature(s) derives from this gene:

Feature NameUnique NameSpeciesType
NO17G00670.1NO17G00670.1-proteinNannochloropsis oceanica (N. oceanica IMET1)polypeptide


The following gene feature(s) are orthologous to this gene:

Feature NameUnique NameSpeciesType
jgi.p|Nanoce1779_2|600153gene_7818Nannochloropsis oceanica (N. oceanica CCMP1779)gene
Naga_100108g12gene9266Nannochloropsis gaditana (N. gaditana B-31)gene


The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
NO17G00670.1NO17G00670.1Nannochloropsis oceanica (N. oceanica IMET1)mRNA


Sequences
The following sequences are available for this feature:

gene sequence

>NO17G00670 ID=NO17G00670|Name=NO17G00670|organism=Nannochloropsis oceanica|type=gene|length=1123bp
ATGGCCAGCGAGGTTTTGTGCATGTCATGGAGGCTATTCGCCGCGACAAT
ACTTGGAAGACGAGCGAGgtgcgttagcaacacttctcccccgggaccgc
aatcgtcgttgtgtcgtgcagctctcgccaagctgaggccgccgttgttt
ttcggacgcgcggtccaaggacatttccccccttgcttttcacgacacag
ccattaagcggaccaatttatggcgaatatgacgcattgaaatttgatgc
gagtgtgtgtccagtcgcagtactgcttggcaaaagcatgcgggtgggga
gcgtggtggccggccatgtcttgaaagttacctccccaacccttcttatc
tttaccacagGGGGGAAAAAACTCAGCGCAAGTCAGCCCTCTACTGGCCC
CATAAGTGCAACCAGAACGACGAAAACAGgtagagtgataaaatctcata
ttatgctccctgtttgccttagcttgtggcgacatcgcaatcagcaacgc
cgtcgggtaggccatctataccgctgccactctgccaagcaacttctcgc
tcaattgaaccttcccgatgccttgattgctaccccctccaccatgccac
tcaatcccccccaaacagAGCCATGGCTACTTCATCCCCACCACCGTCTC
ACATCGACCACGACGCGATTTACCTCCAGCAAGCCCTCATTGAGGCCCAG
CAGGGGCTCGAGGAGGGGGGTATCCCTATCGGGGCCGTCTTGGTCCTCCG
AGAAGCAGGTGGAACCCGGCGAGTCCTAGGGAAAGGCCACAATCGACGCG
TGCAGAAAGGCTCGGCCATCTTGCATGGGGAGATAGATTGCTTGGAAAAC
GCTGGACGGTTGCCTGCTTCTGTGTATAAAATGTGTACCATGTACACCAC
GCTGTCGCCGTGTTCGATGTGTAGTGGGGCGATCCTGTTGTTTGGGATTC
CACGAGTTGTAGTAGGGGAAAACAAGACTTTTATGGGCGCAGAGGACTAT
TTGAGCGCACAGGGGGTCGAGGTAATCGTGAAGCAGGATGAAGGGTGTGT
GCAGATTATGACGGAGTTTATTAAGGAGAATGCAGAGCTGTGGGAGGAGG
ATAGTGGACAGTCGAAGGAGTGA
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protein sequence of NO17G00670.1

>NO17G00670.1-protein ID=NO17G00670.1-protein|Name=NO17G00670.1|organism=Nannochloropsis oceanica|type=polypeptide|length=214bp
MASEVLCMSWRLFAATILGRRARGEKTQRKSALYWPHKCNQNDENRAMAT
SSPPPSHIDHDAIYLQQALIEAQQGLEEGGIPIGAVLVLREAGGTRRVLG
KGHNRRVQKGSAILHGEIDCLENAGRLPASVYKMCTMYTTLSPCSMCSGA
ILLFGIPRVVVGENKTFMGAEDYLSAQGVEVIVKQDEGCVQIMTEFIKEN
AELWEEDSGQSKE*
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Synonyms
Publications