NO13G01270, NO13G01270 (gene) Nannochloropsis oceanica | NanDeSyn: Nannochloropsis Design & Synthesis Initiative

Overview


Name	NO13G01270
Unique Name	NO13G01270
Type	gene
Organism	Nannochloropsis oceanica (N. oceanica IMET1)
Sequence length	1716
Alignment location	chr13:384593..386308 +

Link to JBrowse

Properties


Property Name	Value
Description	Bifunctional dihydrofolate reductase-thymidylate synthase 1

Mutants

Expression

Analysis:

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Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
chr13	genome	chr13:384593..386308 +

Analyses

This gene is derived from or has results from the following analyses


Analysis Name	Date Performed
GSE178672	2023-12-15
PRJNA933693	2023-10-26
PRJNA943449	2023-07-19
GSE149904	2020-10-10
NoIMET1_WT_HS	2020-09-29
GSE139615	2020-03-11
PRJNA241382	2020-03-11
BLASTP analysis of N. oceanica IMET1 genes	2019-07-11
InterPro analysis for N. oceanica IMET1 genes	2019-07-11
PRJNA182180	2019-04-25
Gene prediction for N. oceanica IMET1	2017-10-24

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0006730	one-carbon metabolic process
GO:0055114	oxidation-reduction process
GO:0046654	tetrahydrofolate biosynthetic process
GO:0006231	dTMP biosynthetic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0004146	dihydrofolate reductase activity
GO:0004799	thymidylate synthase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR036926	Thymidate_synth/dCMP_Mease_sf
IPR024072	DHFR-like_dom
IPR012262	DHFR-TS
IPR023451	Thymidate_synth/dCMP_Mease
IPR001796	DHFR_dom
IPR000398	Thymidylate_synthase

Homology

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BLAST of NO13G01270 vs. NCBI_GenBank
Match: EWM21795.1 (bifunctional dihydrofolate reductase-thymidylate synthase [Nannochloropsis gaditana])

HSP 1 Score: 761.9 bits (1966), Expect = 1.400e-216
Identity = 389/554 (70.22%), Postives = 422/554 (76.17%), Query Frame = 0

Query:   18 EGLRGFTIVVAATAKSLGIGKQGQLPWNLPEDMAHFKHLTSSTTLPNKTNAVIMGRRTWQSIPERFRPLRNRLNVVLSRNPAIREQLNLPQGVRVAASLPEALALLSTGEDATAVEKVFVIGGAAVYKEALSSDLCEAIEFTAIDEEGQEGGQGGEREISNNSPTPLFADCDAFFPTIPATVYGLVTSTETLTQNHLHFRFQRFERLNYRALPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDLVREIIEKGNHRGDRTQTGTLSKFGAQLRFSLRNNVFPLLTTKRVFWRGVAEELLWFVSGKTNSNLLKDKKIGIWDGNSSREYLDKIGLSNREEGDLGPVYGFQWRHFGAEYKDMHADYTGKGIDQLANAIETIKKNPEDRRIIVSAWNPSDLGKMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTSLVAQVCGLAPGEFVHTIGDAHVYSNHVEALKEQLTRTPAKFPRLFINPDKKCIDDFVFEDFRIEGYTPQENIKMKMAV 572
            E LRGFTIVVAATAK+ GIGK GQLPWNLP+DM HFK LT+ T++P+K NAVIMGRRTWQSIPE+FRPLRNRLNVVLSRNP IREQLNLPQGVRVA SL  ALALL+ G DA+ VEKVFVIGGAAVY+EA+ S+ CEAIEFT+I+EE                 TPLFADCDA FP IPAT Y LV+ST  L+QN L FRFQR+ERL+                                                                 L LV+EIIE G  RGDRTQTGTLSKFGAQLRFSLR+  FPLLTTKRVFWRGVAEELLWF+SG T++N LK+KKIGIWDGNSSREYLDK+GLS+REEGDLGPVYGFQWRHFGAEY DMHADYTGKGIDQLA AI TIK NPEDRRIIVSAWNP+DLGKMALPPCHMFCQFYVA+GELSCQMYQRSADMGLGVPFNIASYALLT LVAQVCGL  GEF+HTIGDAHVY NHV+ALKEQLTRTP  FP+L INP KK ID F FEDF IEGY P E++KMKMAV
Sbjct:   23 EALRGFTIVVAATAKTFGIGKAGQLPWNLPQDMEHFKRLTACTSVPDKINAVIMGRRTWQSIPEKFRPLRNRLNVVLSRNPGIREQLNLPQGVRVATSLKAALALLAHGNDASVVEKVFVIGGAAVYREAVESEACEAIEFTSIEEE--------------RGATPLFADCDAHFPVIPATEYCLVSSTPVLSQNALRFRFQRYERLD-------------------------------------RGPSADLPVGDGPNKVSGAAGNQEEAQYLRLVQEIIEHGKRRGDRTQTGTLSKFGAQLRFSLRDETFPLLTTKRVFWRGVAEELLWFISGSTDANYLKEKKIGIWDGNSSREYLDKLGLSHREEGDLGPVYGFQWRHFGAEYSDMHADYTGKGIDQLARAITTIKDNPEDRRIIVSAWNPADLGKMALPPCHMFCQFYVADGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQVCGLKAGEFIHTIGDAHVYLNHVDALKEQLTRTPRSFPKLRINPAKKDIDSFTFEDFTIEGYLPHESLKMKMAV 525

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BLAST of NO13G01270 vs. NCBI_GenBank
Match: CBN76613.1 (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 582.0 bits (1499), Expect = 2.000e-162
Identity = 371/600 (61.83%), Postives = 426/600 (71.00%), Query Frame = 0

Query:    6 ATAVAAASTVTKEGLRGFTIVVAATAKSLGIGKQGQLPWNLPEDMAHFKHLTSS----------TTLPNKTNAVIMGRRTWQSIPERFRPLRNRLNVVLSRNPAIREQLNLPQGVRVAASLPEALALLSTGEDATAVEKVFVIGGAAVYKEALSSDLCEAIEFTAIDEEGQEGGQGGEREISNNSPTPLFADCDAFFPTIPATVYGLVTSTETLTQNHLHFRFQRFERLNY------------------------RALPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDLVREIIEKGNHRGDRTQTGTLSKFGAQLRFSLRNNVFPLLTTKRVFWRGVAEELLWFVSGKTNSNLLKDKKIGIWDGNSSREYLDKIGLSNREEGDLGPVYGFQWRHFGAEYKDMHADYTGKGIDQLANAIETIKKNPEDRRIIVSAWNPSDLGKMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTSLVAQVCGLAPGEFVHTIGDAHVYSNHVEALKEQLTRTPAKFPRLFINPDKKCIDDFVFEDFRIEGYTPQENIKMKMAV 572
            A++VA AS  T    R F ++VAATA SLGIGK G LPW L  DMA+FK  TS+              +K NAVIMGR+TWQSIPERFRPL  R NVVLSRNPA R+ L LP+ V +A SL EAL LLS    A+ V+KVFVIGG +VY EA++S+LCE +  T++                       F DCD  FP +    + LV   E   +  +   F  +E +N+                             XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX    L+LV +I++ G  RGDRT TGTLSKFG Q+RFSLR   FPLLTTKRVFWRGVAEELLWFV+G TN+N+LK++ I IWDGN SRE+LD +GL+ REEGDLGPVYGFQWRHFGAEY DMHADYTGKG+DQLA  I+ IK NPEDRRI++SAWNP+DLGKMALPPCHMFCQFYVA GELSCQMYQRSADMGLGVPFNIASYALLT LVAQ CGL  G+FVHTIGDAHVY NHV+AL+EQL R P  FP L IN D+  ID F ++   ++GY+P + IKMKMAV
Sbjct:    2 ASSVAVASPSTS---RSFVVIVAATAGSLGIGKNGALPWRLAADMAYFKRCTSTPXXXXXXXXXXXXXDKINAVIMGRKTWQSIPERFRPLAGRRNVVLSRNPAARDTLRLPKDVLLAGSLTEALDLLSPAA-ASEVDKVFVIGGGSVYAEAVASELCEKVLLTSVTPPAD----------------GRFDDCDTHFPALDPDTFKLVKKGEAQEEKGVELSFDEYESVNHSEAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEMQYLNLVEDILDNGVRRGDRTGTGTLSKFGVQMRFSLREERFPLLTTKRVFWRGVAEELLWFVAGCTNANVLKERGIHIWDGNGSREFLDGLGLTEREEGDLGPVYGFQWRHFGAEYTDMHADYTGKGVDQLAECIQKIKTNPEDRRIVLSAWNPADLGKMALPPCHMFCQFYVAEGELSCQMYQRSADMGLGVPFNIASYALLTRLVAQACGLKAGDFVHTIGDAHVYVNHVDALREQLKRKPRPFPTLSINTDETDIDSFEYKHLEVKGYSPYKTIKMKMAV 581

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BLAST of NO13G01270 vs. NCBI_GenBank
Match: OUS47933.1 (thymidylate synthase/dCMP hydroxymethylase domain-containing protein [Ostreococcus tauri])

HSP 1 Score: 556.6 bits (1433), Expect = 8.900e-155
Identity = 293/560 (52.32%), Postives = 356/560 (63.57%), Query Frame = 0

Query:   20 LRGFTIVVAATAKSLGIGKQGQLPWNLPEDMAHFKHLTSSTTLPNKTNAVIMGRRTWQSIPERFRPLRNRLNVVLSRNPAIREQLN--------LPQGVRVAASLPEALALLSTGEDATAVEKVFVIGGAAVYKEALSSDLCEAIEFTAIDEEGQEGGQGGEREISNNSPTPLFADCDAFFPTIPATVYGLVTSTETLTQNHLHFRFQRFERLNYRALPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDLVREIIEKGNHRGDRTQTGTLSKFGAQLRFSLRNNVFPLLTTKRVFWRGVAEELLWFVSGKTNSNLLKDKKIGIWDGNSSREYLDKIGLSNREEGDLGPVYGFQWRHFGAEYKDMHADYTGKGIDQLANAIETIKKNPEDRRIIVSAWNPSDLGKMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTSLVAQVCGLAPGEFVHTIGDAHVYSNHVEALKEQLTRTPAKFPRLFINPDKKCIDDFVFEDFRIEGYTPQENIKMKMAV 572
            +R F +VVAAT    GIGK   LPW L +DM HFK LTS T   N  NAV+MGR+TW+SIP +FRPL  RLNVVLSR+  + E  +        LP+GV V  ++ +AL+ +S+ E    VEK FVIGGA +Y+EAL S+ CEA+  T ++ +                      +CDAF P I A  + L   ++ +T+N   ++F  +   +  +                                                              LD++REII++G  +GDRT TGT+S FG Q+RF LR + FPLLTTKRVFWRGVAEELLWFV+G+TN+N L +KKI IWDGN SREYLD IGL+ RE GDLGPVYGFQWRHFGAEY +MHADYTGKG+DQLA  I  IK NP DRRI+++AWNP+ L +MALPPCHMFCQFYVANGELSCQMYQRS DMGLGVPFNIASY+LLT ++AQVC L PG+FVH  GD HVYSNHVE LK+QLT  P  FP L INP+KK ID F F+DF I GY P   I+MKMAV
Sbjct:    1 MRAFQVVVAATVDG-GIGKDNGLPWKLTKDMGHFKKLTSETRDANAVNAVVMGRKTWESIPAKFRPLPGRLNVVLSRSGVLAEANDENNNGAETLPEGVLVRKTIDDALSAISSSE--KRVEKTFVIGGAQIYEEALKSERCEAVHLTEVEGD---------------------FECDAFIPKIDADKFKLYGQSKPITENGARYQFLTYVTKDSES--------------------------------------GKFRPIAGELLPAGCDVKHEEHQYLDMIREIIDQGAVKGDRTGTGTISTFGNQMRFDLRRS-FPLLTTKRVFWRGVAEELLWFVAGETNANKLAEKKINIWDGNGSREYLDSIGLTEREVGDLGPVYGFQWRHFGAEYTNMHADYTGKGVDQLAEVINKIKNNPTDRRILLTAWNPAALKEMALPPCHMFCQFYVANGELSCQMYQRSCDMGLGVPFNIASYSLLTCMIAQVCDLKPGDFVHVCGDTHVYSNHVEPLKKQLTCEPRPFPILKINPEKKDIDSFTFDDFEIIGYDPHPKIEMKMAV 497

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BLAST of NO13G01270 vs. NCBI_GenBank
Match: XP_002502546.1 (bifunctional dihydrofolate reductase-thymidylate synthase [Micromonas commoda] >ACO63804.1 bifunctional dihydrofolate reductase-thymidylate synthase [Micromonas commoda])

HSP 1 Score: 555.8 bits (1431), Expect = 1.500e-154
Identity = 297/566 (52.47%), Postives = 352/566 (62.19%), Query Frame = 0

Query:   20 LRGFTIVVAATAKSLGIGKQGQLPWNLPEDMAHFKHLTSSTTLPNKTNAVIMGRRTWQSIPERFRPLRNRLNVVLSRNPAIREQLN--------------LPQGVRVAASLPEALALLSTGEDATAVEKVFVIGGAAVYKEALSSDLCEAIEFTAIDEEGQEGGQGGEREISNNSPTPLFADCDAFFPTIPATVYGLVTSTETLTQNHLHFRFQRFERLNYRALPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDLVREIIEKGNHRGDRTQTGTLSKFGAQLRFSLRNNVFPLLTTKRVFWRGVAEELLWFVSGKTNSNLLKDKKIGIWDGNSSREYLDKIGLSNREEGDLGPVYGFQWRHFGAEYKDMHADYTGKGIDQLANAIETIKKNPEDRRIIVSAWNPSDLGKMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTSLVAQVCGLAPGEFVHTIGDAHVYSNHVEALKEQLTRTPAKFPRLFINPDKKCIDDFVFEDFRIEGYTPQENIKMKMAV 572
            +R F +VVAAT   +GIG QG LPWNLP+DMA+FK +TS    P   NAV+MGR+TW+SIP +FRPL  R+NVVLSR   + E  +              LP+GV +  SL  AL LLS  E    VEKVFVIGGA VY EA++S  C+A+  T               E++  +  P    CDAF P I    + L  S + L +          + L Y  + P                                                           LDL++EI+E+GN +GDRT TGT+SKFG Q+RF LR + FPLLTTKRVFWRGVAEEL+WFV G T++  L+DK I IWDGN SREYLD +GL +REEGDLGPVYGFQWRHFGAEYKDMHADYTG+G+DQLA  I+ IK NP DRRI+++AWNP+ L +MALPPCHMFCQFYVANGELSCQMYQRS DMGLGVPFNIASY+LLT +VAQVCGL PG+FVH  GD HVYSNHVE L +QL   P  FP L INP+KK ID F F DF I  Y P   I M+MAV
Sbjct:    8 VRPFQVVVAATKGEMGIGYQGGLPWNLPKDMAYFKAVTSQVNEPGTKNAVVMGRKTWESIPAKFRPLPGRVNVVLSRGHTLVEAGSENSAPVNGGGKPEVLPEGVFLRPSLEAALELLSAPEHEAGVEKVFVIGGAQVYAEAMASPHCQALHLT---------------EVTPPADEPEKYKCDAFLPQIDPAKFKLYASAKPLREKD----GATIQFLTYFGVDP------------------------------GTGKFRSPGSKVLPAGAVAKGVRHEEMQYLDLIKEIMEEGNVKGDRTGTGTISKFGCQMRFDLRRS-FPLLTTKRVFWRGVAEELIWFVKGCTSAKELQDKDIHIWDGNGSREYLDSVGLGHREEGDLGPVYGFQWRHFGAEYKDMHADYTGQGVDQLAEVIDKIKNNPNDRRILLTAWNPAALKEMALPPCHMFCQFYVANGELSCQMYQRSCDMGLGVPFNIASYSLLTCMVAQVCGLKPGDFVHCCGDTHVYSNHVEPLYKQLENEPRPFPTLKINPEKKDIDSFEFSDFEIVDYDPHPKIAMQMAV 523

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BLAST of NO13G01270 vs. NCBI_GenBank
Match: XP_003074642.2 (Thymidylate synthase [Ostreococcus tauri] >CEF96887.1 Thymidylate synthase [Ostreococcus tauri])

HSP 1 Score: 555.1 bits (1429), Expect = 2.600e-154
Identity = 292/560 (52.14%), Postives = 355/560 (63.39%), Query Frame = 0

Query:   20 LRGFTIVVAATAKSLGIGKQGQLPWNLPEDMAHFKHLTSSTTLPNKTNAVIMGRRTWQSIPERFRPLRNRLNVVLSRNPAIREQLN--------LPQGVRVAASLPEALALLSTGEDATAVEKVFVIGGAAVYKEALSSDLCEAIEFTAIDEEGQEGGQGGEREISNNSPTPLFADCDAFFPTIPATVYGLVTSTETLTQNHLHFRFQRFERLNYRALPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDLVREIIEKGNHRGDRTQTGTLSKFGAQLRFSLRNNVFPLLTTKRVFWRGVAEELLWFVSGKTNSNLLKDKKIGIWDGNSSREYLDKIGLSNREEGDLGPVYGFQWRHFGAEYKDMHADYTGKGIDQLANAIETIKKNPEDRRIIVSAWNPSDLGKMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTSLVAQVCGLAPGEFVHTIGDAHVYSNHVEALKEQLTRTPAKFPRLFINPDKKCIDDFVFEDFRIEGYTPQENIKMKMAV 572
            +R F +VVAAT    GIGK   LPW L +DM HFK LTS T   N  NAV+MGR+TW+SIP +FRPL  RLNVVLSR+  + E  +        LP+GV V  ++ +AL+ +S+ E    VEK FVIGGA +Y+EAL S+ CEA+  T ++ +                      +CD F P I A  + L   ++ +T+N   ++F  +   +  +                                                              LD++REII++G  +GDRT TGT+S FG Q+RF LR + FPLLTTKRVFWRGVAEELLWFV+G+TN+N L +KKI IWDGN SREYLD IGL+ RE GDLGPVYGFQWRHFGAEY +MHADYTGKG+DQLA  I  IK NP DRRI+++AWNP+ L +MALPPCHMFCQFYVANGELSCQMYQRS DMGLGVPFNIASY+LLT ++AQVC L PG+FVH  GD HVYSNHVE LK+QLT  P  FP L INP+KK ID F F+DF I GY P   I+MKMAV
Sbjct:    3 MRAFQVVVAATVDG-GIGKDNGLPWKLTKDMGHFKKLTSETRDANAVNAVVMGRKTWESIPAKFRPLPGRLNVVLSRSGVLAEANDENNNGAETLPEGVLVRKTIDDALSAISSSE--KRVEKTFVIGGAQIYEEALKSERCEAVHLTEVEGD---------------------FECDVFIPKIDADKFKLYGQSKPITENGARYQFLTYVTKDSES--------------------------------------GKFRPIAGELLPAGCDVKHEEHQYLDMIREIIDQGAVKGDRTGTGTISTFGNQMRFDLRRS-FPLLTTKRVFWRGVAEELLWFVAGETNANKLAEKKINIWDGNGSREYLDSIGLTEREVGDLGPVYGFQWRHFGAEYTNMHADYTGKGVDQLAEVINKIKNNPTDRRILLTAWNPAALKEMALPPCHMFCQFYVANGELSCQMYQRSCDMGLGVPFNIASYSLLTCMIAQVCDLKPGDFVHVCGDTHVYSNHVEPLKKQLTCEPRPFPILKINPEKKDIDSFTFDDFEIIGYDPHPKIEMKMAV 499

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BLAST of NO13G01270 vs. NCBI_GenBank
Match: XP_012201157.1 (bifunctional dihydrofolate reductase-thymidylate synthase 2 [Saprolegnia parasitica CBS 223.65] >KDO28037.1 bifunctional dihydrofolate reductase-thymidylate synthase 2 [Saprolegnia parasitica CBS 223.65])

HSP 1 Score: 553.1 bits (1424), Expect = 9.900e-154
Identity = 286/549 (52.09%), Postives = 356/549 (64.85%), Query Frame = 0

Query:   24 TIVVAATAKSLGIGKQGQLPWNLPEDMAHFKHLTSSTTLPNKTNAVIMGRRTWQSIPERFRPLRNRLNVVLSRNPAIREQLNLPQGVRVAASLPEALALLSTGEDATAVEKVFVIGGAAVYKEALSSDLCEAIEFTAIDEEGQEGGQGGEREISNNSPTPLFADCDAFFPTIPATVYGLVTSTETL-TQNHLHFRFQRFERLNYRALPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDLVREIIEKGNHRGDRTQTGTLSKFGAQLRFSLRNNVFPLLTTKRVFWRGVAEELLWFVSGKTNSNLLKDKKIGIWDGNSSREYLDKIGLSNREEGDLGPVYGFQWRHFGAEYKDMHADYTGKGIDQLANAIETIKKNPEDRRIIVSAWNPSDLGKMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTSLVAQVCGLAPGEFVHTIGDAHVYSNHVEALKEQLTRTPAKFPRLFINPDKKCIDDFVFEDFRIEGYTPQENIKMKMAV 572
            +++VA    + GIG +GQLPW L  D+  F+ LT+ST    K NAV+MGR+TW+S+P + RPL  R+NVVLSRNPA+R  L+   GV  A +L EAL  L      T VE VF+IGG ++Y+EA     C     T +D E                      +CDAFFP   A+     TS   +  +  + F++ + +R +                                                                 L+L+++I+++G H+GDRT TGT+S FGAQ+RFSLR++VFPLLTTKRVFW+GVAEELLWF+SG TN+  L+DK I IWDGN SRE+LDK+GL++RE GDLGPVYGFQWRHFGA+Y DM+ DYTG+G+DQLA+ I  IK +P DRRI++SAWNPSDLG MALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLT ++AQVCGL  G+F+H IGDAHVYSNHVE L+EQL RTP+ FP L INP K  ID F F+DF IEGYTPQ+ IKM+MA+
Sbjct:    5 SMIVAVARATNGIGIKGQLPWRLAGDLKRFRELTTSTADATKQNAVVMGRKTWESLPLKHRPLPGRVNVVLSRNPAVRAALSAQTGVLAAGNLDEALGAL------TGVEHVFIIGGQSLYEEAAQHPRCTRAYLTLVDGE---------------------YECDAFFPKSLASCGFAETSVSPVQIEKDISFQYIQLDRKH------------------------------------------------------------EELQYLELIQKILDEGVHKGDRTGTGTISLFGAQMRFSLRHDVFPLLTTKRVFWKGVAEELLWFISGDTNAKTLQDKGIKIWDGNGSREFLDKLGLTHREAGDLGPVYGFQWRHFGAKYVDMYTDYTGQGVDQLADVIHKIKHSPNDRRILLSAWNPSDLGLMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTRMIAQVCGLKAGDFIHVIGDAHVYSNHVEPLQEQLKRTPSPFPMLKINPAKMDIDKFTFDDFTIEGYTPQKTIKMEMAI 466

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BLAST of NO13G01270 vs. NCBI_GenBank
Match: XP_009040691.1 (hypothetical protein AURANDRAFT_38942 [Aureococcus anophagefferens] >EGB04584.1 hypothetical protein AURANDRAFT_38942 [Aureococcus anophagefferens])

HSP 1 Score: 551.2 bits (1419), Expect = 3.800e-153
Identity = 296/548 (54.01%), Postives = 344/548 (62.77%), Query Frame = 0

Query:   24 TIVVAATAKSLGIGKQGQLPWNLPEDMAHFKHLTSSTTLPNKTNAVIMGRRTWQSIPERFRPLRNRLNVVLSRNPAIREQLNLPQGVRVAASLPEALALLSTGEDATAVEKVFVIGGAAVYKEALSSDLCEAIEFTAIDEEGQEGGQGGEREISNNSPTPLFADCDAFFPTIPATVYGLVTSTETLTQNHLHFRFQRFERLNYRALPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDLVREIIEKGNHRGDRTQTGTLSKFGAQLRFSLRNNVFPLLTTKRVFWRGVAEELLWFVSGKTNSNLLKDKKIGIWDGNSSREYLDKIGLSNREEGDLGPVYGFQWRHFGAEYKDMHADYTGKGIDQLANAIETIKKNPEDRRIIVSAWNPSDLGKMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTSLVAQVCGLAPGEFVHTIGDAHVYSNHVEALKEQLTRTPAKFPRLFINPDKKCIDDFVFEDFRIEGYTPQENIKMKMAV 572
            T +VAA A S GIGK G LPW L  DM +FK +TS+     KTNA +MGR+TW SIP +FRPL  R NVVLSRNP  RE+L LP  V V  SL  A+  L+   D   + +VFVIGG  VY  AL+   C  +  T+ID+                     FADCDA FP      +    S E L +N + +RF+   R   +  PP                                                           LDL+ EI+++G  RGDRT TGT+S FGA++ FSLRN+ FPLLTTKRVFWRGVAEEL+WFVSG TN  LL DK I IWDGN SRE+LD+ GL +REE DLGPVYGFQWRHFGA+Y DMH DY G+G+DQLA  +ETIK++P  RRI++SAWNP+DL +MALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLT +VAQ  GL PG+FVH IGDAHVYSNHV+ALK QL R P  FP L INP K  IDDF F DF +EGY P   I MKMAV
Sbjct:   18 TCIVAAVAGSYGIGKDGTLPWKLAGDMKYFKKVTSAAP-EGKTNACVMGRKTWLSIPPKFRPLGGRKNVVLSRNPNAREELGLPPDVFVCPSLDAAVDALAGEAD---LGEVFVIGGGQVYASALADARCTKVLLTSIDD-----------------AAGAFADCDAVFPDPAKAGFASAASGEALVENGVAYRFETLTRPAKK--PP--------------------------------LRESNGPPAPPPANEKPAAPNHEEKQYLDLIAEILDRGVRRGDRTGTGTISIFGARMSFSLRNDRFPLLTTKRVFWRGVAEELVWFVSGSTNGKLLADKGIHIWDGNGSREFLDRRGLGHREEMDLGPVYGFQWRHFGAKYVDMHKDYAGEGVDQLAACVETIKQDPNSRRIVLSAWNPADLDEMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTRMVAQATGLKPGDFVHVIGDAHVYSNHVDALKVQLQREPRPFPTLTINPAKTGIDDFEFADFTVEGYKPYPKIDMKMAV 510

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BLAST of NO13G01270 vs. NCBI_GenBank
Match: XP_007514117.1 (predicted protein [Bathycoccus prasinos] >CCO15554.1 predicted protein [Bathycoccus prasinos])

HSP 1 Score: 550.4 bits (1417), Expect = 6.400e-153
Identity = 296/577 (51.30%), Postives = 359/577 (62.22%), Query Frame = 0

Query:   13 STVTKEGLRGFTIVVAATAKSLGIGKQGQLPWNLPEDMAHFKHLTSSTTLPNKTNAVIMGRRTWQSIPERFRPLRNRLNVVLSRNPAIREQLN-----------------LPQGVRVAASLPEALALLSTGEDATAVEKVFVIGGAAVYKEALSSDLCEAIEFTAIDEEGQEGGQGGEREISNNSPTPLFADCDAFFP-TIPATVYGLVTSTETLTQNHLHFRFQRFERLNYRALPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDLVREIIEKGNHRGDRTQTGTLSKFGAQLRFSLRNNVFPLLTTKRVFWRGVAEELLWFVSGKTNSNLLKDKKIGIWDGNSSREYLDKIGLSNREEGDLGPVYGFQWRHFGAEYKDMHADYTGKGIDQLANAIETIKKNPEDRRIIVSAWNPSDLGKMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTSLVAQVCGLAPGEFVHTIGDAHVYSNHVEALKEQLTRTPAKFPRLFINPDKKCIDDFVFEDFRIEGYTPQENIKMKMAV 572
            +T TK+ L+ F +VVA+     GIGK   LPW L ++M HFK LT+  + P   NAVIMGR+TW+SIPE+FRPL  RLNVVLS +  + E+ N                 LP+GV   AS+ EAL  LSTGE    +EKVFVIGGA VY+EAL S+ CEA+  T +            +  +   P   FA CDAF P  + +  + L +S++ +      +    +   N  A                                                              L+L+REII++GN +GDRT TGT+SKFG  +R+ LR + FPLLT+KRVFWRGVAEELLWFV G TN+N LK+K +GIWDGN SREYLD IGL++RE  DLGPVYGFQWRHF AEY DMHADY GKG+DQLA  I  IK NP DRRI+++AWNP+ L +MALPPCHMFCQFYVANGELSCQMYQRS DMGLGVPFNIASY+LLT ++AQVC L PG+FVH +GDAHVY+NHVE L+ QL   P  FP+L INPD K ID F FEDF I GY P   I+MKMAV
Sbjct:    3 ATPTKDDLKKFQVVVASDELG-GIGKNQTLPWKLSKEMKHFKSLTADVSKPMTQNAVIMGRKTWESIPEKFRPLPGRLNVVLSASGRVYEKSNKENGGPGEDDAEGGAKCLPEGVLARASIEEALETLSTGEYKDIIEKVFVIGGAKVYEEALKSEQCEAVHLTEV------------KPPAGKDPKEHFA-CDAFIPLPLDSEKFRLYSSSKLVKDGECSYTTLTYVAQNGPA------------------------------------GKFRVEAEKILPPKILKEQKHEEMQYLELIREIIDEGNVKGDRTGTGTISKFGCSMRYDLRRS-FPLLTSKRVFWRGVAEELLWFVKGSTNANELKEKGVGIWDGNGSREYLDSIGLNHREVNDLGPVYGFQWRHFNAEYTDMHADYAGKGVDQLAEVIHKIKNNPNDRRILLTAWNPAALKEMALPPCHMFCQFYVANGELSCQMYQRSCDMGLGVPFNIASYSLLTCMIAQVCDLKPGDFVHVLGDAHVYANHVEPLETQLKNEPRPFPQLKINPDVKDIDGFKFEDFEIIGYDPCPKIEMKMAV 528

[Close]

BLAST of NO13G01270 vs. NCBI_GenBank
Match: XP_003056897.1 (bifunctional dihydrofolate reductase-thymidylate synthase [Micromonas pusilla CCMP1545] >EEH58542.1 bifunctional dihydrofolate reductase-thymidylate synthase [Micromonas pusilla CCMP1545])

HSP 1 Score: 547.7 bits (1410), Expect = 4.200e-152
Identity = 291/565 (51.50%), Postives = 346/565 (61.24%), Query Frame = 0

Query:   21 RGFTIVVAATAKSLGIGKQGQLPWNLPEDMAHFKHLTSSTTLPNKTNAVIMGRRTWQSIPERFRPLRNRLNVVLSRNPAIREQLNLPQGV--------------RVAASLPEALALLSTGEDATAVEKVFVIGGAAVYKEALSSDLCEAIEFTAIDEEGQEGGQGGEREISNNSPTPLFADCDAFFPTIPATVYGLVTSTETLTQNHLHFRFQRFERLNYRALPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDLVREIIEKGNHRGDRTQTGTLSKFGAQLRFSLRNNVFPLLTTKRVFWRGVAEELLWFVSGKTNSNLLKDKKIGIWDGNSSREYLDKIGLSNREEGDLGPVYGFQWRHFGAEYKDMHADYTGKGIDQLANAIETIKKNPEDRRIIVSAWNPSDLGKMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTSLVAQVCGLAPGEFVHTIGDAHVYSNHVEALKEQLTRTPAKFPRLFINPDKKCIDDFVFEDFRIEGYTPQENIKMKMAV 572
            R F +VVAAT   +GIG  G LPW LP+DMA+FK +T++   P K NAVIMGR+TW+SIP +FRPL  RLNVVLS++ A+ E                       +  SL  ALA L  GE A  VEK+FV+GGA VY EA++S  C+A+  T               E++    TP    CD   P +    + L  S   + +        + + L+Y A                                                              LDL+R+IIE GN +GDRT TGT+SKFG  +RF LR + FPLLTTKRVFWRGVAEELLWFV G+TN+  L+DK I IWDGN SREYLD +GLS+REE DLGPVYGFQWRHFGAEY DMHA+Y GKG+DQLA  I+ IK NP DRRI+++AWNP+ L +MALPPCHMFCQFYVANGELSCQMYQRS DMGLGVPFNIASY+LLT ++AQVCGL PG+FVH +GD HVYSNHVE LK+QL   P  FP L INP K  ID F F DF IEGY P   I+MKMAV
Sbjct:   10 RPFQVVVAATKGDMGIGLDGALPWRLPKDMAYFKSVTATVAEPGKRNAVIMGRKTWESIPAKFRPLPGRLNVVLSKSGALVESTGSENAAPAXXXXXXXXXXXXLLQPSLEAALATLGAGEHAENVEKIFVVGGAQVYAEAMASPACQAVHIT---------------EVTPPKDTPDAFKCDTHMPPVDMEKFKLYASAPPVNEK----EGAKIQFLSYVA------------------------------KDAATGKFRIPGSVSLPPGAVANGVRHEELQYLDLIRDIIESGNVKGDRTGTGTISKFGTSMRFDLRRS-FPLLTTKRVFWRGVAEELLWFVGGRTNAKELQDKDIHIWDGNGSREYLDSVGLSHREEQDLGPVYGFQWRHFGAEYTDMHANYDGKGVDQLAEVIDKIKNNPNDRRILLTAWNPAALKEMALPPCHMFCQFYVANGELSCQMYQRSCDMGLGVPFNIASYSLLTCMIAQVCGLKPGDFVHVMGDTHVYSNHVEPLKKQLLCEPRPFPTLKINPAKTDIDSFEFSDFTIEGYDPHPKIEMKMAV 524

[Close]

BLAST of NO13G01270 vs. NCBI_GenBank
Match: BAJ98915.1 (predicted protein [Hordeum vulgare subsp. vulgare])

HSP 1 Score: 545.8 bits (1405), Expect = 1.600e-151
Identity = 285/551 (51.72%), Postives = 351/551 (63.70%), Query Frame = 0

Query:   21 RGFTIVVAATAKSLGIGKQGQLPWNLPEDMAHFKHLTSSTTLPNKTNAVIMGRRTWQSIPERFRPLRNRLNVVLSRNPAIREQLNLPQGVRVAASLPEALALLSTGEDATAVEKVFVIGGAAVYKEALSSDLCEAIEFTAIDEEGQEGGQGGEREISNNSPTPLFADCDAFFPTIPATVYGLVTSTETLTQNHLHFRFQRFERLNYRALPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDLVREIIEKGNHRGDRTQTGTLSKFGAQLRFSLRNNVFPLLTTKRVFWRGVAEELLWFVSGKTNSNLLKDKKIGIWDGNSSREYLDKIGLSNREEGDLGPVYGFQWRHFGAEYKDMHADYTGKGIDQLANAIETIKKNPEDRRIIVSAWNPSDLGKMALPPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTSLVAQVCGLAPGEFVHTIGDAHVYSNHVEALKEQLTRTPAKFPRLFINPDKKCIDDFVFEDFRIEGYTPQENIKMKMAV 572
            R + +VVAAT + +GIGK G LPW LP D+  FK LT +T+ P+K NAVIMGR+TW+SIP +FRPL  RLNV+L+R+ +        + V    SL  +L LL++   ++ +EK F+IGG  V +E+L++  CEAI  T I+                        +CD F P I   ++    S+  + +N++   F  F R+   A                                                              L+LV++I+  G  + DRT TGT+SKFG Q+RF+LR N FPLLTTKRVFWRGV EELLWF+SG TN+ +L++K I IWDGN+SREYLD IGLS REEGDLGPVYGFQWRHFGAEY DMHADYTGKG DQLA+ I  IK NP+DRRII+SAWNP+DL KMALPPCHMF QFYV NGELSCQMYQRSADMGLGVPFNIASY+LLT ++AQVC L+PGEFVH IGDAHVYS HV+AL+EQL + P  FP L INP KK ID FV  DF++  Y P + I+MKMAV
Sbjct:   16 RKYQVVVAAT-REMGIGKDGALPWKLPSDLKFFKDLTMATSDPSKKNAVIMGRKTWESIPTKFRPLPGRLNVILTRSGS--SDYAKAENVVTCGSLDSSLELLASAPYSSTIEKAFLIGGGQVLRESLNASACEAIHLTDIEST---------------------IECDTFIPPIDPLIFHPWYSSSPVVENNIRHSFATFVRVRKSA-------------------------EEANDPNVNDTKKEKFEIQIFSFLPKMIFEKHDEYQYLNLVQDILRTGARKNDRTGTGTISKFGCQMRFNLRKN-FPLLTTKRVFWRGVLEELLWFISGSTNAKVLQEKNIRIWDGNASREYLDSIGLSQREEGDLGPVYGFQWRHFGAEYSDMHADYTGKGFDQLADVINKIKNNPDDRRIILSAWNPTDLKKMALPPCHMFAQFYVENGELSCQMYQRSADMGLGVPFNIASYSLLTCMIAQVCDLSPGEFVHVIGDAHVYSTHVQALEEQLQKQPKPFPILKINPLKKDIDSFVASDFKLACYDPHQKIEMKMAV 516

The following BLAST results are available for this feature:

BLAST of NO13G01270 vs. NCBI_GenBank

BLAST of NO13G01270 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes)
Total hits: 20


Match Name	E-value	Identity	Description
EWM21795.1	1.400e-216	70.22	bifunctional dihydrofolate reductase-thymidylate s...	[more]
CBN76613.1	2.000e-162	61.83	conserved unknown protein [Ectocarpus siliculosus]	[more]
OUS47933.1	8.900e-155	52.32	thymidylate synthase/dCMP hydroxymethylase domain-...	[more]
XP_002502546.1	1.500e-154	52.47	bifunctional dihydrofolate reductase-thymidylate s...	[more]
XP_003074642.2	2.600e-154	52.14	Thymidylate synthase [Ostreococcus tauri] >CEF9688...	[more]
XP_012201157.1	9.900e-154	52.09	bifunctional dihydrofolate reductase-thymidylate s...	[more]
XP_009040691.1	3.800e-153	54.01	hypothetical protein AURANDRAFT_38942 [Aureococcus...	[more]
XP_007514117.1	6.400e-153	51.30	predicted protein [Bathycoccus prasinos] >CCO15554...	[more]
XP_003056897.1	4.200e-152	51.50	bifunctional dihydrofolate reductase-thymidylate s...	[more]
BAJ98915.1	1.600e-151	51.72	predicted protein [Hordeum vulgare subsp. vulgare]	[more]

Pages

Relationships

This gene is member of the following syntenic_region feature(s):


Feature Name	Unique Name	Species	Type
nonsL134	nonsL134	Nannochloropsis oceanica (N. oceanica IMET1)	syntenic_region
ncniR026	ncniR026	Nannochloropsis oceanica (N. oceanica IMET1)	syntenic_region
ngnoR070	ngnoR070	Nannochloropsis oceanica (N. oceanica IMET1)	syntenic_region

This gene is orthologous to the following gene feature(s):


Feature Name	Unique Name	Species	Type
NSK001769	NSK001769	Nannochloropsis salina (N. salina CCMP1776)	gene

The following polypeptide feature(s) derives from this gene:


Feature Name	Unique Name	Species	Type
NO13G01270.1	NO13G01270.1-protein	Nannochloropsis oceanica (N. oceanica IMET1)	polypeptide

The following gene feature(s) are orthologous to this gene:


Feature Name	Unique Name	Species	Type
jgi.p\|Nanoce1779_2\|115886	gene_6882	Nannochloropsis oceanica (N. oceanica CCMP1779)	gene
Naga_100065g11	gene8639	Nannochloropsis gaditana (N. gaditana B-31)	gene

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Species	Type
NO13G01270.1	NO13G01270.1	Nannochloropsis oceanica (N. oceanica IMET1)	mRNA

Sequences

The following sequences are available for this feature:

gene sequence
protein sequence

gene sequence

>NO13G01270 ID=NO13G01270|Name=NO13G01270|organism=Nannochloropsis oceanica|type=gene|length=1716bp
ATGTCTCCCCTCGCTGCCACCGCTGTTGCTGCCGCATCGACCGTCACAAA
GGAGGGTCTGAGGGGTTTTACCATCGTCGTGGCCGCCACCGCCAAGTCCC
TGGGCATTGGCAAGCAAGGCCAACTTCCTTGGAACCTTCCAGAAGACATG
GCACATTTCAAACACCTCACATCGTCCACCACATTGCCCAACAAGACCAA
CGCCGTCATCATGGGCCGTCGGACGTGGCAATCCATCCCCGAGAGATTCC
GCCCCCTGCGAAATCGTCTCAACGTCGTCCTTTCCCGCAATCCCGCAATC
CGCGAGCAACTAAACCTCCCACAAGGTGTGCGTGTTGCGGCCTCGTTACC
CGAGGCATTGGCGTTGTTGTCCACGGGGGAGGATGCAACGGCGGTCGAGA
AAGTGTTTGTGATCGGCGGGGCTGCTGTTTACAAAGAAGCTTTATCCTCC
GACCTATGCGAAGCCATTGAATTCACCGCCATCGATGAGGAAGGGCAGGA
GGGAGGGCAGGGAGGAGAGAGGGAGATTTCGAATAACAGCCCGACGCCGC
TGTTCGCGGATTGTGACGCCTTTTTCCCTACCATCCCTGCCACTGTTTAT
GGACTCGTCACCTCGACCGAGACCTTGACCCAGAATCATTTGCACTTCCG
TTTTCAGCGCTTTGAACGTTTGAATTACAGGGCTTTACCACCTTTCTATG
GCAGCAGTAGCAGCAGCAGCAGCAACAACCACAACAACAACAACAACAAC
AACAACAACAACAACAACAACAACAACAACAACAACAACAACAAAACTAA
GGGCAGTGCGTCGGCTCCGGCTGGGGGAGGAAAGAAGGAAGAGGAAAGGA
ACGAAGAAGAGCAGCAATACCTGGATCTTGTCCGCGAAATCATTGAGAAG
GGCAACCATCGGGGGGATCGAACACAGACAGGCACCTTGTCCAAGTTTGG
AGCACAGCTTCGATTTAGCCTCAGGAACAATGTGTTTCCTCTCTTAACGA
CTAAGAGGGTATTTTGGCGGGGGGTCGCGGAGGAGTTGCTGTGGTTTGTG
AGTGGCAAGACAAACTCGAATCTATTGAAAGACAAAAAGATTGGAATTTG
GGACGGAAACTCTAGCAGGGAGTACCTGGACAAAATAGGGTTGAGCAATC
GGGAGGAGGGAGACCTAGGACCGGTGTACGGCTTCCAGTGGCGGCATTTT
GGGGCTGAATACAAGGATATGCACGCGGACTACACCGGGAAGGGCATCGA
TCAGCTGGCAAATGCAATTGAGACGATAAAGAAAAATCCGGAAGATCGAC
GGATCATTGTCTCAGCCTGGAATCCTTCGGATCTGGGGAAGATGGCGTTG
CCCCCGTGTCACATGTTTTGTCAGTTTTATGTGGCAAATGGGGAGCTGTC
CTGCCAGATGTACCAACGCAGCGCGGATATGGGTTTGGGTGTGCCGTTTA
ATATTGCTTCGTATGCATTGCTCACCAGCTTGGTGGCGCAGGTGTGTGGG
TTGGCGCCAGGGGAGTTCGTGCACACAATTGGGGATGCTCACGTTTACTC
GAATCACGTCGAGGCCTTGAAGGAGCAGTTGACGCGAACCCCGGCCAAGT
TTCCTCGGCTCTTCATTAACCCGGACAAGAAATGCATCGACGACTTTGTG
TTTGAGGATTTTCGGATTGAGGGGTACACGCCGCAGGAGAACATCAAAAT
GAAGATGGCGGTGTAG

protein sequence of NO13G01270.1

>NO13G01270.1-protein ID=NO13G01270.1-protein|Name=NO13G01270.1|organism=Nannochloropsis oceanica|type=polypeptide|length=572bp
MSPLAATAVAAASTVTKEGLRGFTIVVAATAKSLGIGKQGQLPWNLPEDM
AHFKHLTSSTTLPNKTNAVIMGRRTWQSIPERFRPLRNRLNVVLSRNPAI
REQLNLPQGVRVAASLPEALALLSTGEDATAVEKVFVIGGAAVYKEALSS
DLCEAIEFTAIDEEGQEGGQGGEREISNNSPTPLFADCDAFFPTIPATVY
GLVTSTETLTQNHLHFRFQRFERLNYRALPPFYGSSSSSSSNNHNNNNNN
NNNNNNNNNNNNNNKTKGSASAPAGGGKKEEERNEEEQQYLDLVREIIEK
GNHRGDRTQTGTLSKFGAQLRFSLRNNVFPLLTTKRVFWRGVAEELLWFV
SGKTNSNLLKDKKIGIWDGNSSREYLDKIGLSNREEGDLGPVYGFQWRHF
GAEYKDMHADYTGKGIDQLANAIETIKKNPEDRRIIVSAWNPSDLGKMAL
PPCHMFCQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTSLVAQVCG
LAPGEFVHTIGDAHVYSNHVEALKEQLTRTPAKFPRLFINPDKKCIDDFV
FEDFRIEGYTPQENIKMKMAV*

Synonyms

Publications