NO12G02130, NO12G02130 (gene) Nannochloropsis oceanica

BLAST of NO12G02130 vs. NCBI_GenBank
Match: EWM25837.1 (maleylacetate reductase [Nannochloropsis gaditana])

HSP 1 Score: 665.6 bits (1716), Expect = 1.400e-187
Identity = 357/621 (57.49%), Postives = 444/621 (71.50%), Query Frame = 0

Query:    1 MQEFVVEAPPARIFFGPGCIKCVGATEIRKLGLHRLLVLGSHRALTGTPALVELQAAIKDLVVGVIQDVEWYNSPEALVDKANEVSSVDADGLLVIGGGSPLGLAKALAQLMSQFS------PSRLAIVSIPTTYSGSEVTAYHGIHLSADGMPQ----------------KFAIRNRVVRPLLVLYDAQLLLGLPRALAISSSFNALAHAFEALWVSRTPSPQSQRAAKMSIETIFKYLPALDTTDQ--ESEEGLEVVNELLHAAYLGAHSLDTEDLGLQHQLAHVLIGTYNLPHAEAHMLLLPHVIHFYEETWAGAGKNTTPLTDIQLPLTSSPMLGMACHVFDFQRTLLRSQPPLNLKDLGLQESDLEGVIDTLIEYDGLSKDPEFPWAKPALLILLQHAWAGDRPLLERHAQSPQVSSVPKVPRVSYLSNDDFRGQDPEAAAKIVARRQGNLLNLDRVLMHAPAVAQGWNAMFGSLRSGLQLIDGRLRELVILRVAVLNRAYYEYFQHYPVLLAEGGTVAEAEALGDWQGEAARGIFGDRDKAVLAYVDEMTRHVQVQQATAETLRAVLPVGNSESYMVELTALCAGYNMVSRFLEALQLTPETTNLAELPHMPVDV 598
            M+EFVV+ PPARI FGPGC+  VGA EIR LG  RL+++GS R+L   PA+V LQ+ + DLVVG I  V+     + + +   EV  + AD L+V+GGG+ LGLAKALA L+   S      PS L I+SIPTTYSGSEVT+YHG+ +  +   Q                K A+RN+ VRP LVLYDA  L  LP  L ISS FNALAHAFEALWV+  PSPQ+  AA  S+  I +Y+  LD + +  ++ E L VVNELL+ AYL A+SLDTEDLGLQH+LAHVL GTY++PHA+AHMLLLPHV+ FYE+ WA  G+ TTPL+ ++L    S      C +FD QR+L+ S+PPL+L+D+GL + DLEGV+DTL+E+ G+    + P +KP LL LLQHA +G+ P  E    S       + PR+SYLS+ DFR +DPEAA+KI+ARR G LL+LDRVLMHAP VAQGWNAMFGSLRSGL+ IDGRLRELVILRVAV+N+AYYEYFQHYPV L EGG+V EAEALG+W+ EA++ +FG +D+AVLAYVDEMT  +QVQ  T + L A       ES++VELTALCAGYNMVSRFLEAL LT E+ N A LP MPVD+
Sbjct:    1 MREFVVKGPPARIIFGPGCVSRVGAAEIRTLGCKRLVMVGSVRSLKNIPAVVALQSELGDLVVGTIPRVKENVYGDLVEEAGKEVGRLRADALVVVGGGTALGLAKALANLLPLSSTGPSQMPSPLTIISIPTTYSGSEVTSYHGVSVVLNPAAQALIDKDANKPHGIKARKVAVRNQAVRPALVLYDAHFLQSLPYPLTISSLFNALAHAFEALWVNPMPSPQACWAASESVNIILRYIGNLDPSGRVADASESLVVVNELLYGAYLAAYSLDTEDLGLQHRLAHVLGGTYSVPHAQAHMLLLPHVLRFYEDAWADGGEKTTPLSHLKLGQAPSTQSLARC-IFDIQRSLVHSKPPLSLQDVGLGQPDLEGVVDTLLEHGGIPVRQDVPISKPLLLGLLQHAISGESPPWEGRPAS-------RTPRISYLSDQDFRAKDPEAASKIMARRHGQLLHLDRVLMHAPTVAQGWNAMFGSLRSGLRSIDGRLRELVILRVAVVNQAYYEYFQHYPVFLKEGGSVPEAEALGNWRSEASKHLFGAKDRAVLAYVDEMTGRIQVQDKTVKILFATASTEAPESFVVELTALCAGYNMVSRFLEALHLTAESENSAALPRMPVDI 613          

BLAST of NO12G02130 vs. NCBI_GenBank
Match: CDR38177.1 (RHTO0S03e05160g1_1 [Rhodotorula toruloides])

HSP 1 Score: 174.5 bits (441), Expect = 1.000e-39
Identity = 131/387 (33.85%), Postives = 193/387 (49.87%), Query Frame = 0

Query:    1 MQEFVVEAPPARIFFGPGCIKCVGATEIRKLGLHRLLVLGSHRALTGTPALVELQAAIKDLVVGVIQDVEWYNSPEALVDKANEVSSVDADGLLVIGGGSPLGLAKALAQLMSQFSPSRLAIVSIPTTYSGSEVTAYHG-IHLSADGMPQKFAIRNRVVRPLLVLYDAQLLLGLPRALAISSSFNALAHAFEALWVSRTPSPQSQRAAKMSIETIFKYLPALDTTDQESEEGLEVVNELLHAAYLGAHSLDTEDLGLQHQLAHVLIGTYNLPHAEAHMLLLPHVIHFYEETWAGAGKNTTPLTDIQLPLTSSPMLGMACHVFDFQRTLLRSQPPLNLKDLGLQESDLEGVIDTLIEYDGLSKDPEFPWAKPALLILLQHAWAGDRPL 387
            MQ FV  A PAR+ FG G  K V A E+ +LG  R LVL + + +    A  ++QA + DL VG+      +   E  +D   +  ++ AD  + +GGGS +GLAKALA  +   + ++L IV+IPTTY+GSE T   G   +  DG P K   +   V P  ++YD +L L LP  + ++S  NA+AHA EALW     +P     A   I+ I + LP +    Q S++G     + L  A+     L    + L H+L H L G++N+PHAE H ++LPH + +     A A  +             +  LG+A            +  P +LK LG +E DLE   +  +       +P  P  K  LL LL++A+ G RPL
Sbjct:    1 MQPFVYIALPARVVFGFGTAKQV-AQEVTRLGCSRALVLTTPQQVA---AGEQVQADLGDLNVGIYSKAAMHTPLEVTLDALAQAKALGADCCVAVGGGSTIGLAKALA--LHSANSAKLKIVAIPTTYAGSEATPIIGQTEVGKDGKPLKTTQKTMKVLPEAIVYDVELTLSLPPQMTVTSGLNAIAHAVEALWAVEA-NPIISSLAIQGIKAIARSLPLIKAGPQ-SKDGRA---DALFGAWACGTCLGAVGMSLHHKLCHTLGGSFNMPHAETHTVILPHAVAYNAPFAADAVSSV------------AKALGVANASQGLYDLAKENGAPYSLKALGFKEEDLERAAE--LAASAPYPNPA-PLKKEKLLALLRNAYEGVRPL 361          

BLAST of NO12G02130 vs. NCBI_GenBank
Match: KUJ86435.1 (putative maleylacetate reductase [Halomonas sp. 54_146])

HSP 1 Score: 172.2 bits (435), Expect = 5.000e-39
Identity = 127/382 (33.25%), Postives = 194/382 (50.79%), Query Frame = 0

Query:    4 FVVEAPPARIFFGPGCIKCVGATEIRKLGLHRLLVLGSHRALTGTPALVELQAAIKDLVVGVIQDVEWYNSPEALVDKANEVSSVDADGLLVIGGGSPLGLAKALAQLMSQFSPSRLAIVSIPTTYSGSEVTAYHGIHLSADGMPQKFAIRNRVVRPLLVLYDAQLLLGLPRALAISSSFNALAHAFEALWVSRTPSPQSQRAAKMSIETIFKYLPALDTTDQESEEGLEVVNELLHAAYLGAHSLDTEDLGLQHQLAHVLIGTYNLPHAEAHMLLLPHVIHFYEETWAGAGKNTTPLTDIQLPLTSSPMLGMACHVFDFQRTLLRSQPPLNLKDLGLQESDLEGVIDTLIEYDGLSKDPEFPWAKPALLILLQHAWAGDRP 386
            F+  A PARI FGPG +  +   E+R+LG  + LVL +            L   + +L VGV  +   +   E         +++ AD ++ +GGGS +GL KA+A        +    V I TTY+GSEVTA  G   + DG+  K  +++  + P +V+YDA+L LGLP + +++S+ NA+AHA E L+       ++   A M++E I     AL    Q+  E LE  N  L+ A+L    L T  + L H+L H L G++NLPHAE H ++LPH + +          N     ++  P        +   ++DF + L   Q PL LK+LGL E+DL+ V D  ++    +     P  + A+  LLQ AW+G RP
Sbjct:    5 FLYIANPARIVFGPGSLAQIN-DEVRQLGCAQALVLSTPSRRKDAE---RLNQQLGNLGVGVFSEAAMHTPVEVTERALQAYAAMGADCVIALGGGSTIGLGKAIAY------RNNAPQVVIATTYAGSEVTAILG--QTEDGV--KTTVKHESILPEVVIYDAELTLGLPVSTSVTSALNAMAHALEGLYAKE----RNPITALMAVEGIGVLKEALPIIVQQ-PENLEARNNALYGAWLCGTVLGTVGMALHHKLCHTLGGSFNLPHAETHAIMLPHTMAY----------NAQAAAEMLAPAAELFAGDLGGGLYDFAQGL---QAPLALKELGLAEADLDKVADLAVQNPYWNPQ---PIERQAVRELLQRAWSGTRP 351          

BLAST of NO12G02130 vs. NCBI_GenBank
Match: WP_013959719.1 (maleylacetate reductase [Cupriavidus necator] >AEI82688.1 maleylacetate reductase TftE (plasmid) [Cupriavidus necator N-1])

HSP 1 Score: 169.9 bits (429), Expect = 2.500e-38
Identity = 126/394 (31.98%), Postives = 195/394 (49.49%), Query Frame = 0

Query:    1 MQEFVVEAPPARIFFGPGCIKCVGATEIRKLGLHRLLVLGSHRALTGTPALVELQAAIKDLV----VGVIQDVEWYNSPEALVDKANEVSSVDADGLLVIGGGSPLGLAKALAQLMSQFSPSRLAIVSIPTTYSGSEVTAYHGIHLSADGMPQKFAIRNRVVRPLLVLYDAQLLLGLPRALAISSSFNALAHAFEALWVSRTPSPQSQRAAKMSIETIFKYLPALDTTDQESEEGLEVVNELLHAAYLGAHSLDTEDLGLQHQLAHVLIGTYNLPHAEAHMLLLPHVIHFYEETWAGAGKNTTPLTDIQLPLTSSPMLGMACHVFDFQRTLLR----SQPPLNLKDLGLQESDLEGVIDTLIEYDGLSKDPEFPWAKPALLILLQHAWAGDRPL 387
            M  FV  A P+R+ FG G    + A EI +LG HR L+L        TP  VEL      L+     G+    + +         +     + AD L+  GGGS +GLAKA+A        S L +V++PTTY+GSE+T  +GI        +K    +  V P  V+YD +L LGLP  ++++S  NA+AHA EAL+ +R  +P  +  A++ I  + + +PA+    +   E  +  ++ L+ A+L   +L    + L H+L H L GT+NLPHAE H ++LPH + +         + +TP         +  +LG A    D  R L      +  P  L+D+G QE+D+    D  + +   +     P A+ ALL LLQ A+ G+ P+
Sbjct:    1 MNPFVYHANPSRVVFGMGTFAQLPA-EIERLGCHRSLLL-------STPHQVELARDAATLLGSAAAGIFDQAQMHVPAGVAAQASKAAKKIGADCLVATGGGSTIGLAKAIA------LESGLPVVAVPTTYAGSEMTPIYGI----TDQGRKRTGSDARVLPRTVIYDPELTLGLPVVISVTSGINAIAHAAEALY-ARDGNPIMETLAELGIRALARAIPAI----RSDVEDRQARSDALYGAWLCGTTLGHVGMALHHKLCHTLGGTFNLPHAETHTIVLPHALAY--------NRKSTP--------RAMQILGRALASEDGPRALYELARDNGAPTALRDIGFQEADIAVAADIALTHPYWN---PAPLAREALLALLQRAYRGEPPI 352          

BLAST of NO12G02130 vs. NCBI_GenBank
Match: WP_102633682.1 (maleylacetate reductase [Paraburkholderia rhynchosiae] >PMS29273.1 maleylacetate reductase [Paraburkholderia rhynchosiae])

HSP 1 Score: 169.9 bits (429), Expect = 2.500e-38
Identity = 126/389 (32.39%), Postives = 191/389 (49.10%), Query Frame = 0

Query:    1 MQEFVVEAPPARIFFGPGCIKCVGATEIRKLGLHRLLVLGSHRALTGTPALVE-LQAAIKDLVVGVIQDVEWYNSPEALVDKANEVSSVDADGLLVIGGGSPLGLAKALAQLMSQFSPSRLAIVSIPTTYSGSEVTAYHGIHLSADGMPQKFAIRNRVVRPLLVLYDAQLLLGLPRALAISSSFNALAHAFEALWVSRTPSPQSQRAAKMSIETIFKYLPALDTTDQESEEGLEVVNELLHAAYLGAHSLDTEDLGLQHQLAHVLIGTYNLPHAEAHMLLLPHVIHFYEETWAGAGKNTTPLTDIQLPLTSSPMLGMACHVFDFQRTLLRSQPPLNLKDLGLQESDLEGVIDTLIEYDGLSKDPEFPWAKP------ALLILLQHAWAG 383
            M+ F+ +A PAR+ FG G    + A E ++LGL RLL+L       G  AL E  +    + VVG+      +   +        V  + ADG++ IGGGS +GL KA+A        + L  + +PTTY+GSE+T   G   + DG+  K   R+  + P  V+YD  L L LP  ++  S+FNA+AHA EAL+   T +P     A+  + T+ + LP L  T  +      V +++L+ A+L ++ L    +GL H+L H L G +NLPHAE H ++LP+V+ F       A        +   P+ +   L  AC +            P+ LKD+G+ E  + G +  L+           P+A P       L  LL+HAWAG
Sbjct:    1 MESFIYQAQPARVIFGSGTAAQL-ADEAQRLGLKRLLIL----CTPGQAALAERARQWAGERVVGIFAGATMHTPVDVTERALRVVQDLHADGVVSIGGGSTIGLGKAIA------LRTDLPQIVLPTTYAGSEMTPILG--ETKDGL--KTTQRSPRILPETVIYDVDLSLSLPSPISALSAFNAIAHAVEALYAPDT-NPIISLMAQEGVRTLVRALPQLIETPHDP----AVRSDMLYGAWLCSYCLGNTTMGLHHKLCHTLGGAFNLPHAETHTIVLPYVLEFNAPVIPDAMARLCHAMNDDDPVNTLLALERACRI------------PMALKDIGMPEEGIAGAVQQLLAN---------PYANPRPLETIELTTLLEHAWAG 348          

BLAST of NO12G02130 vs. NCBI_GenBank
Match: WP_034287162.1 (maleylacetate reductase [Bacillus sp. 72])

HSP 1 Score: 169.5 bits (428), Expect = 3.200e-38
Identity = 133/393 (33.84%), Postives = 201/393 (51.15%), Query Frame = 0

Query:    1 MQEFVVEAPPARIFFGPGCIKCVGATEIRKLGLHRLLVLGSHRALTGTPALVELQAAIKDLVVGVIQDVE---WYNSPEALVDKANE-VSSVDADGLLVIGGGSPLGLAKALAQLMSQFSPSRLAIVSIPTTYSGSEVTAYHGIHLSADGMPQKFAIRNRVVRPLLVLYDAQLLLGLPRALAISSSFNALAHAFEALWVSRTPSPQSQRAAKMSIETIFKYLPALDTTDQESEEGLEVVNELLHAAYLGAHSLDTEDLGLQHQLAHVLIGTYNLPHAEAHMLLLPHVIHFYEETWAGAGKNTTPLTDIQLPLTSSPMLGMACHVFDFQRTLLRSQPPLNLKDLGLQESDLEGVIDTLIEYDGLSKDPEF---PWAKPALLILLQHAWAGDRPL 387
            M+ F+ EA  AR+ FGPG IK V  TE+ K+   R LV+        TP   EL   + DL+  +   +      + P   V+KA E V ++  D L+ IGGGS +GLAKA+A   S      L IV+IPTTY+GSE+T   G  L+ +G+  K   ++ VV+P  V+YD  L + LP  + ++S  NA+AH  E L+     +P     A+  I  +   LPA+          +E  +E L+  +LG   L +  + L H+L HVL G+YNLPHAE H ++LP+ I      W  A   +  +  I   L       +A  +FD   +L     P +L ++G++ESDL+   +        +K+P +   P  +  +  LL++A+AG RPL
Sbjct:    1 MKSFLYEAQAARVIFGPGEIKQV-QTELEKISAKRALVI-------STPGQEELANKVSDLIGDLCAGIHAKAVQHVPTEKVEKALEIVRALHVDSLVPIGGGSSIGLAKAIALNTS------LPIVAIPTTYAGSEMTPIWG--LTENGI--KKTGKDLVVKPKTVIYDPNLTVNLPTRVTVTSGMNAIAHCVEGLYAENV-NPIISLLAEEGIRALHSSLPAI----LADSYNIEARSEALYGCWLGGTVLGSVGMALHHKLCHVLGGSYNLPHAETHSVMLPYAI------WYNATHASKAIKSIARALRVEEE-DVAGSLFDLTTSL---GVPTSLAEIGMKESDLDQAAEL------AAKNPYYNPRPIEQQTIRKLLEYAFAGKRPL 354          

BLAST of NO12G02130 vs. NCBI_GenBank
Match: PBK65917.1 (iron-containing alcohol dehydrogenase [Armillaria solidipes])

HSP 1 Score: 169.5 bits (428), Expect = 3.200e-38
Identity = 129/388 (33.25%), Postives = 191/388 (49.23%), Query Frame = 0

Query:    1 MQEFVVEAPPARIFFGPGCIKCVGATEIRKLGLHRLLVLGSHRALTGTPALVELQAAIKDLVVGVIQDVEWYNSPEALVDKANEVSSVDADGLLVIGGGSPLGLAKALAQLMSQFSPSRLAIVSIPTTYSGSEVTAYHGIHLSADGMPQKFAIRNRVVRPLLVLYDAQLLLGLPRALAISSSFNALAHAFEALWVSRTPSPQSQRAAKMSIETIFKYLPALDTTDQESEEGLEVVNELLHAAYLGAHSLDTEDLGLQHQLAHVLIGTYNLPHAEAHMLLLPHVIHFYEETWAGAGKNTTPLTDIQLPLTSSPMLGMACHVFDFQRTLLRSQPPLNLKDLGLQESDLEGVIDTLIEYDGLSKDP---EFPWAKPALLILLQHAWAGDRP 386
            M+ FV  A P+R+ FG G +  V   EI  LG  + L+L + R +     L EL   + D  VG+  +   +       D    V  + AD ++ +GGGS +GLAKA+A        + L  + IPTTYSGSE TA  G   + +G+  K   +   V P +++YD  L L LP  + I+S  NA+AHA EAL+ S+  SP +   A+  I +I K LP +          L+  +E L+ A+     L    +GL H+L H L GT+N+PHAE H ++LPH + +       A +    +  +            A  +FD  ++L     P  LK+LG++E DLE   D        SK P     P  K  +L+LL++A+AG RP
Sbjct:    1 MKSFVYNALPSRVVFGNGTLSQV-VQEITNLGCSKALILTTPRQVAQGERLKEL---VGDKTVGIYTNATMHTPTNVTDDAVAVVQKLGADVVVAVGGGSTIGLAKAIA------LRTDLPQIVIPTTYSGSEATAVIG--QTENGL--KTTQKTLKVLPEVIIYDISLTLTLPPRVTITSGLNAIAHAVEALY-SKEASPITDTLAEQGILSIAKALPVI----MNDSGNLDARSEALYGAWACGTCLGAVGMGLHHKLCHTLGGTFNMPHAETHTVILPHSMAYNAPYAQEAMEKVAKILGVS---------NAAQGIFDLSKSL---GVPSTLKELGMKEEDLEKAADI------ASKSPYPNPAPLEKEKMLLLLKNAYAGVRP 351          

BLAST of NO12G02130 vs. NCBI_GenBank
Match: PRQ78033.1 (iron-containing alcohol dehydrogenase [Rhodotorula toruloides])

HSP 1 Score: 169.1 bits (427), Expect = 4.200e-38
Identity = 132/391 (33.76%), Postives = 191/391 (48.85%), Query Frame = 0

Query:    1 MQEFVVEAPPARIFFGPGCIKCVGATEIRKLGLHRLLVLGSHRALTGTPALVELQAAIKDLVVGVIQDVEWYNSPEALVDKANEVSSVDADGLLVIGGGSPLGLAKALAQLMSQFSPSRLAIVSIPTTYSGSEVTAYHG-IHLSADGMPQKFAIRN-----RVVRPLLVLYDAQLLLGLPRALAISSSFNALAHAFEALWVSRTPSPQSQRAAKMSIETIFKYLPALDTTDQESEEGLEVVNELLHAAYLGAHSLDTEDLGLQHQLAHVLIGTYNLPHAEAHMLLLPHVIHFYEETWAGAGKNTTPLTDIQLPLTSSPMLGMACHVFDFQRTLLRSQPPLNLKDLGLQESDLEGVIDTLIEYDGLSKDPEFPWAKPALLILLQHAWAGDRP 386
            MQ FV  A PAR+ FG G  K V A E+ +LG  R LVL + + +    A  ++QA + DL VG+      +   E  +D   +  ++DAD  + +GGGS +GLAKALA  +     ++L IV+IPTTY+GSE T   G   +  DG P K   +       +V P +++YD  L L L   + + S  NA+AHA EALW     +P     A   IE I + L  L   D +S++G     + L  A+     L    + L H+L H L G++N+PHAE H ++LPH + +     A A                +  LG+A            +  P +LK LG +E DLE   +  I       +P  P  K  LL+LL++A+ G RP
Sbjct:    1 MQPFVYTALPARVVFGFGTAKQV-AHEVARLGCSRALVLTTPQQVA---AGEQVQADLGDLNVGIYSKAAMHTPLEVTLDALAQAKALDADCCVAVGGGSTIGLAKALA--LHSPDSAKLKIVAIPTTYAGSEATPIIGQTEIGKDGKPLKTTQKTMKASLSLVLPEVIVYDVDLTLTLSPQMTVISGLNAIAHAVEALWAVEA-NPIISTLAVQGIEAIARSL-LLIKADSQSKDGRA---DALFGAWACGTCLGAVGMSLHHKLCHTLGGSFNMPHAETHTVILPHAVAYNAPFAADAVSRV------------AKALGVANAAQGLYDLSKENGAPYSLKSLGFKEEDLERAAE--IAARAPYPNPA-PLVKEKLLVLLRNAYEGVRP 365          

BLAST of NO12G02130 vs. NCBI_GenBank
Match: WP_063974891.1 (maleylacetate reductase [Domibacillus aminovorans] >OAH55734.1 maleylacetate reductase [[Bacillus] aminovorans])

HSP 1 Score: 168.7 bits (426), Expect = 5.500e-38
Identity = 137/398 (34.42%), Postives = 204/398 (51.26%), Query Frame = 0

Query:    1 MQEFVVEAPPARIFFGPGCIKCVGATEIRKLGLHRLLVLGSHRALTGTPALVELQAAIKDLV----VGV----IQDVEWYNSPEALVDKANE-VSSVDADGLLVIGGGSPLGLAKALAQLMSQFSPSRLAIVSIPTTYSGSEVTAYHGIHLSADGMPQKFAIRNRVVRPLLVLYDAQLLLGLPRALAISSSFNALAHAFEALWVSRTPSPQSQRAAKMSIETIFKYLPALDTTDQESEEGLEVVNELLHAAYLGAHSLDTEDLGLQHQLAHVLIGTYNLPHAEAHMLLLPHVIHFYEETWAGAGKNTTPLTDIQLPLTSSPMLGMACHVFDFQRTLLRSQPPLNLKDLGLQESDLEGVIDTLIEYDGLSKDPEF---PWAKPALLILLQHAWAGDRPL 387
            M+ F+ EA  AR+ FGPG IK V  TE+ K+   R LV+        TP   EL   + DL+    VG+    +Q V     P   V+KA E V ++  D L+ IGGGS +GLAKA+A   S      L IV+IPTTY+GSE+T   G  L+ +G+  K   ++ VV+P  V+YD  L + LP  + ++S  NA+AH  E L+     +P     A+  I  +   LPA+          +EV +E L+  +LG   L +  + L H+L HVL G+YNLPHAE H ++LP+ I      W  A   +  +  I   L       +A  +FD   +L     P +L ++G++ESDL+   +  +      K+P +      + A+  LL++A+ G RPL
Sbjct:    1 MKSFLYEAQAARVIFGPGEIKQV-QTELEKISAKRALVI-------STPGQEELANKVSDLIGDLCVGIHAKAVQHV-----PTEKVEKALEIVRALHVDSLVPIGGGSSIGLAKAIALNTS------LPIVAIPTTYAGSEMTPIWG--LTENGI--KKTGKDLVVKPKTVIYDPNLTVNLPTLVTVTSGMNAIAHCVEGLYAENV-NPIISLLAEEGIRALHSSLPAI----LADPYNIEVRSEALYGCWLGGTVLGSVGMALHHKLCHVLGGSYNLPHAETHSVMLPYAI------WYNAAHASKAIKSIARALRVEEE-DVAGSLFDLTESL---GVPTSLAEIGMKESDLDQAAELAV------KNPYYNPRSIEQRAIRKLLEYAFTGKRPL 354          

BLAST of NO12G02130 vs. NCBI_GenBank
Match: WP_094619100.1 (MULTISPECIES: maleylacetate reductase [Rhodococcus] >OZC83745.1 maleylacetate reductase [Rhodococcus sp. 06-412-2C] >OZC93931.1 maleylacetate reductase [Rhodococcus sp. 06-412-2B])

HSP 1 Score: 168.7 bits (426), Expect = 5.500e-38
Identity = 132/383 (34.46%), Postives = 199/383 (51.96%), Query Frame = 0

Query:    3 EFVVEAPPARIFFGPGCIKCVGATEIRKLGLHRLLVLGSHRALTGTPALVELQAAIKDLVVGVIQDVEWYNSPEALVDKANEVSSVDADGLLVIGGGSPLGLAKALAQLMSQFSPSRLAIVSIPTTYSGSEVTAYHGIHLSADGMPQKFAIRNRVVRPLLVLYDAQLLLGLPRALAISSSFNALAHAFEALWVSRTPSPQSQRAAKMSIETIFKYLPALDTTDQESEEGLEVVNELLHAAYLGAHSLDTEDLGLQHQLAHVLIGTYNLPHAEAHMLLLPHVIHFYEETWAGAGKNTTPLTDIQLPLTSSPMLGMACHVFDFQRTLLRSQPPLNLKDLGLQESDLEGVIDTLIEYDGLSKDPEFPWAKPALLILLQHAWAGDRP 386
            +F  EA P R+ FG G +  + A E+  LGL R+LVL S  +  GT     + + + D  VGV  D   +   E     + E  S+ ADG + +GGGS +GL KA+A +        L IV++PTTY+GSE+T   G  L+ DG  +K   R+  V P  V+YD +L  GLP  ++++S  NA+AHA EAL+     SP     A+  +  +   LPA+     +S    +  ++ L+ A+L   +L    + L H+L H L GT NLPHA  H ++LPHV+ F +    GA    T L+   LP +  P    A  ++D  R L     P +L +LG++E+D++ + D   +    S     P  + A   +L+ AWAGD+P
Sbjct:    4 DFAYEANPMRVRFGAGSLSAL-ADELDHLGLRRVLVLCS-PSQKGTAE--RIASMLGDRCVGVRADAVMHVPSEVAKRSSAEALSLGADGCVTVGGGSSIGLGKAIALVHG------LPIVAVPTTYAGSEMTPVWG--LTTDG--RKETGRDIRVLPRSVVYDPELTTGLPVDISVTSGINAVAHAVEALYAPDI-SPIVALMAEDGVRALASALPAIVADHLDS----QARSDALYGAWLCGATLGATTMSLHHKLCHSLGGTLNLPHAPTHTVVLPHVLAFNQSAAPGA---VTALSR-ALPGSGDP----ATRLWDLCRGL---GAPRSLAELGMKETDIDRIAD---DVTSRSYGNPRPVTREAATAILRAAWAGDQP 353