NO12G00320, NO12G00320 (gene) Nannochloropsis oceanica

Overview
NameNO12G00320
Unique NameNO12G00320
Typegene
OrganismNannochloropsis oceanica (N. oceanica IMET1)
Sequence length1873
Alignment locationchr12:112068..113940 -

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Properties
Property NameValue
DescriptionEndoglucanase a
Mutants
Expression

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
chr12genomechr12:112068..113940 -
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
PRJNA7699582024-08-13
GSE1786722023-12-15
PRJNA9336932023-10-26
PRJNA9434492023-07-19
GSE1499042020-10-10
NoIMET1_WT_HS2020-09-29
GSE1396152020-03-11
PRJNA2413822020-03-11
BLASTP analysis of N. oceanica IMET1 genes2019-07-11
InterPro analysis for N. oceanica IMET1 genes2019-07-11
PRJNA1821802019-04-25
Gene prediction for N. oceanica IMET12017-10-24
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR008979Galactose-bd-like
Homology
BLAST of NO12G00320 vs. NCBI_GenBank
Match: EWM25981.1 (endoglucanase a [Nannochloropsis gaditana])

HSP 1 Score: 269.2 bits (687), Expect = 1.300e-68
Identity = 121/160 (75.62%), Postives = 140/160 (87.50%), Query Frame = 0
Query:   32 LVLALLPLFAVPQSLYGTLPFADEKIQGPQTIPGKVWLAYFDQGGPDVSYHSYDGKNHGSCELNPCTGTNAIYKNVFRKDEGASTSYTKPPNGGWTGDRFSANNSQVPVDQMYWGWNAIGNWVRLSLLVKKGGNYTLSLFGSSNSGGALTLTVDDYLQKG 192
            LV+A   + A  QSLYGT PFADEK+QGPQ IPGKVW+A+FD+GG +V+YHSYD  NHGSCELNPCTG +A+YKNVFRKDEGASTSYTKPPNGGW GDRF+ NNSQVP+DQMYWGWNA+GNWVR+SLLV++ G+YT+SLFG+SNSGG L LTVDDY   G
Sbjct:   11 LVMASCQIGAGIQSLYGTSPFADEKMQGPQNIPGKVWMAWFDRGGQNVAYHSYDDVNHGSCELNPCTGVDAVYKNVFRKDEGASTSYTKPPNGGWPGDRFAGNNSQVPIDQMYWGWNALGNWVRVSLLVQEPGDYTVSLFGTSNSGGTLLLTVDDYASHG 170          
BLAST of NO12G00320 vs. NCBI_GenBank
Match: XP_005854847.1 (hypothetical protein NGA_0367900 [Nannochloropsis gaditana CCMP526] >EKU21514.1 hypothetical protein NGA_0367900 [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 131.0 bits (328), Expect = 5.400e-27
Identity = 64/93 (68.82%), Postives = 72/93 (77.42%), Query Frame = 0
Query:  164 GNYTLSLFGSSNSGGALTLTVDDYLQKGKAVTTTATIPATDGYHQWALFPDIAVLPSLPPGLALLAVNVTGIYAYKEGSQFGNLLWFDFHWKG 257
            G+YT+SLFG+SNSGG L LTVDDY   G   + TA IP TDGYH+W+LF DIAVLP+L  G ALL VNVTGI  YKEGSQFGNLLW DF  +G
Sbjct:    8 GDYTVSLFGTSNSGGTLLLTVDDYASHGTEASVTAAIPPTDGYHRWSLFQDIAVLPALRRGPALLTVNVTGIDNYKEGSQFGNLLWLDFVRRG 100          
BLAST of NO12G00320 vs. NCBI_GenBank
Match: WP_051978626.1 (hypothetical protein [Edaphobacter aggregans])

HSP 1 Score: 117.5 bits (293), Expect = 6.200e-23
Identity = 74/193 (38.34%), Postives = 103/193 (53.37%), Query Frame = 0
Query:   47 YGTLPFADEKIQGPQTIPGKVWLAYFDQGGPDVSYHSYDGKNHGSCELNPCTGTNAIYKNVFRKDEGASTSYTKPPNGGWTGDRFSANNSQV---PVDQMYWGWNAIGNWVRLSLLVKKGGNYTLSLFGSSNSGGALTLTVDDYLQKGK---AVTTTATIP-ATDGYHQWALFPDIAVLPSLPPGLALLAVNV 233
            Y   P+ D+K + PQTIPG V  A++D+GG  V+YH  D  NHGS ELNP  G+   Y + FR  E    SYTK  N   + DR   N   +   P +Q+Y GW   G W  +S+ V   G YT  L  +SN GG++++ V+ +   G+     T  A  P A   +H W L P++  L  LP G +LL ++V
Sbjct:    5 YKGAPYLDDKQRVPQTIPGVVECAFYDKGGEGVAYHDTDPVNHGSGELNPADGS---YLHEFRMHEAVDISYTKFNN---SPDRTDDNPWTIVLPPANQLYVGWTEPGEWFNISVDVHHAGTYTADLLYTSNRGGSISIDVNGHPASGELKIPTTYNAADPVAWRQWHHWNLAPNLVKL-QLPAGRSLLTIHV 190          
BLAST of NO12G00320 vs. NCBI_GenBank
Match: OJV44199.1 (hypothetical protein BGO25_10605 [Acidobacteriales bacterium 59-55])

HSP 1 Score: 115.5 bits (288), Expect = 2.400e-22
Identity = 83/230 (36.09%), Postives = 115/230 (50.00%), Query Frame = 0
Query:   34 LALLPLFA-VPQSLYGTLPFADE-KIQGPQTIPGKVWLAYFDQGGPDVSYHSYDGKNHGSCELNPCTGTNAIYKNVFRKDEGASTSYTKPPNGGWTGDRFSANNSQVPVDQMYWGWNAIGNWVRLSLLVKKGGNYTLSLFGSSNSGGALTLTVDDYLQKGKAVTTTATIPATDG---------YHQWALFPDIAVLPSLPPGLALLAVNVTGIYAYKEGSQFGNLLWFDF 253
            +A+ P FA  P   Y   P+ D+    G Q+IPG+V  A +D+GG  V+YH  + KN GS  LNP  GT   Y N FR DEG   SYTK  N     D    +  Q P  Q+Y GW   G W  +++   + G Y+  L  +SN GGA+++ ++     GKA +    IP+T           +H W L P I  L  LP G ++L V++      K G+   NL +FDF
Sbjct:    1 MAVAPAFAGTPPKGYKGTPYKDDHHPNGAQSIPGRVECALYDRGGEGVAYHDTEAKNLGSGTLNPADGT---YLNQFRMDEGVDISYTKFHNAPDRIDDNPYDLVQPPDHQLYVGWTEPGEWFNVTVQAARAGVYSADLLYTSNRGGAISVDLN-----GKAASGPLNIPSTFNPADPIAWRQWHHWNLAPAIMKL-HLPAGKSVLTVHI-----LKGGNM--NLAYFDF 214          
BLAST of NO12G00320 vs. NCBI_GenBank
Match: SPE27393.1 (conserved hypothetical protein [Candidatus Sulfotelmatomonas gaucii])

HSP 1 Score: 113.2 bits (282), Expect = 1.200e-21
Identity = 80/224 (35.71%), Postives = 107/224 (47.77%), Query Frame = 0
Query:   47 YGTLPFADEKIQ-GPQTIPGKVWLAYFDQGGPDVSYHSYDGKNHGSCELNPCTGTNAIYKNVFRKDEGASTSYTK----PPNGGWTGDRFSANNSQVPVDQMYWGWNAIGNWVRLSLLVKKGGNYTLSLFGSSNSGGALTLTVDDYLQKGKAVTTTATIPAT---------DGYHQWALFPDIAVLPSLPPGLALLAVNVTGIYAYKEGSQFGNLLWFDFHWKG 257
            Y   P+ D +   GPQ IPGKV+ AY+D+GG  V+YH  D KN+GS  LNP  G+   Y N FR +EG   SYTK    PP      D    +  Q P +  Y GW   G W  +++ VK  G Y+  L  +SN GG ++L V+     G+  T+   I +T           +H W + P I     L   LA    NV  ++   EG    NL +FDF   G
Sbjct:   32 YKGSPYEDSRYHGGPQKIPGKVYCAYYDRGGEGVAYHDSDAKNNGSGALNPADGS---YLNEFRINEGVDISYTKMDRNPPI-----DNTQYDKLQPPANMFYVGWTVPGEWFNITIDVKASGWYSADLLYTSNRGGTISLDVN-----GEPATSELKIESTFDSSDPVAWRQWHHWNIAPGI-----LKVKLA-RGTNVLTVHILTEGQM--NLAYFDFKKAG 234          
BLAST of NO12G00320 vs. NCBI_GenBank
Match: WP_105489074.1 (hypothetical protein [Candidatus Sulfotelmatomonas gaucii])

HSP 1 Score: 113.2 bits (282), Expect = 1.200e-21
Identity = 80/224 (35.71%), Postives = 107/224 (47.77%), Query Frame = 0
Query:   47 YGTLPFADEKIQ-GPQTIPGKVWLAYFDQGGPDVSYHSYDGKNHGSCELNPCTGTNAIYKNVFRKDEGASTSYTK----PPNGGWTGDRFSANNSQVPVDQMYWGWNAIGNWVRLSLLVKKGGNYTLSLFGSSNSGGALTLTVDDYLQKGKAVTTTATIPAT---------DGYHQWALFPDIAVLPSLPPGLALLAVNVTGIYAYKEGSQFGNLLWFDFHWKG 257
            Y   P+ D +   GPQ IPGKV+ AY+D+GG  V+YH  D KN+GS  LNP  G+   Y N FR +EG   SYTK    PP      D    +  Q P +  Y GW   G W  +++ VK  G Y+  L  +SN GG ++L V+     G+  T+   I +T           +H W + P I     L   LA    NV  ++   EG    NL +FDF   G
Sbjct:    4 YKGSPYEDSRYHGGPQKIPGKVYCAYYDRGGEGVAYHDSDAKNNGSGALNPADGS---YLNEFRINEGVDISYTKMDRNPPI-----DNTQYDKLQPPANMFYVGWTVPGEWFNITIDVKASGWYSADLLYTSNRGGTISLDVN-----GEPATSELKIESTFDSSDPVAWRQWHHWNIAPGI-----LKVKLA-RGTNVLTVHILTEGQM--NLAYFDFKKAG 206          
BLAST of NO12G00320 vs. NCBI_GenBank
Match: WP_020710045.1 (hypothetical protein [Acidobacteriaceae bacterium KBS 83])

HSP 1 Score: 112.5 bits (280), Expect = 2.000e-21
Identity = 78/214 (36.45%), Postives = 106/214 (49.53%), Query Frame = 0
Query:   50 LPFADEK-IQGPQTIPGKVWLAYFDQGGPDVSYHSYDGKNHGSCELNPCTGTNAIYKNVFRKDEGASTSYTKPPNGGWTGDRFSANNSQVPVDQMYWGWNAIGNWVRLSLLVKKGGNYTLSLFGSSNSGGALTLTVDDYLQKGKAVTTTATIPATDG---------YHQWALFPDIAVLPSLPPGLALLAVNVTGIYAYKEGSQFGNLLWFDFH 254
            +P+ D +   G QTIPG+V  AY+D+GG  VSYH  D KN GS  LNP  GT   Y N FR  EG  TSYTK  +     D         P   +Y GW   G W  +++ V + G Y   L  +SN GGA+++ V+     G+ VT    I +T           +H W L P + V   LP G ++L V++       +G+   NL +FDFH
Sbjct:    1 MPYQDSRHAGGGQTIPGRVMCAYYDRGGEGVSYHDSDAKNSGSGGLNPADGT---YLNEFRMHEGVDTSYTKYHD---AIDNNPFEKVHPPEGLLYVGWTEPGEWFNITVNVPRAGVYAGDLLYTSNRGGAISIDVN-----GENVTGPVAIASTFNKADPIAWRQWHHWNLAPGL-VKVRLPKGKSVLTVHILA-----QGNM--NLAYFDFH 195          
BLAST of NO12G00320 vs. NCBI_GenBank
Match: WP_011523872.1 (hypothetical protein [Candidatus Koribacter versatilis] >ABF42071.1 hypothetical protein Acid345_3070 [Candidatus Koribacter versatilis Ellin345])

HSP 1 Score: 110.9 bits (276), Expect = 5.800e-21
Identity = 82/231 (35.50%), Postives = 111/231 (48.05%), Query Frame = 0
Query:   30 FFLVLALLP--LFA-VPQSLYGTLPFADEKIQ-GPQTIPGKVWLAYFDQGGPDVSYHSYDGKNHGSCELNPCTGTNAIYKNVFRKDEGASTSYTKPPNGGWTGDRFSANNSQVPVDQMYWGWNAIGNWVRLSLLVKKGGNYTLSLFGSSNSGGALTLTVDDYLQKGKAVTTTATIPATD----GYHQWALFPDIAVLPSLPPGLALLAVNVTGIYAYKEGSQFGNLLWFDF 253
            FF+  +++P  LF+  P S Y   P+ D + Q G Q IPGKV+ AY+D GG  V+YH  D +NHGS ELNP  G+   Y N FR  EG   SYTK        D    N    P D  Y GW     W  +++ V + G Y      +SN GG +++ V+     G    TT   PA       +H W +  D+  + +L  G  +L V+V        GS   NL +FDF
Sbjct:    9 FFVAASMIPATLFSQSPSSEYQGSPYHDSRYQAGAQKIPGKVFCAYYDLGGEGVAYHDTDPQNHGSGELNPADGS---YLNEFRIHEGVDISYTKFNRKPNLIDDNPFNKVVPPADLPYVGWTEPSEWFNVTVEVAQTGTYAADFLYTSNRGGTISIDVNGKDATGPLDITTTNDPADPLPWRQWHHWNVAGDLFKV-TLSKGKNILTVHV-----LTNGSM--NLAYFDF 228          
BLAST of NO12G00320 vs. NCBI_GenBank
Match: WP_058186623.1 (hypothetical protein [Terracidiphilus gabretensis])

HSP 1 Score: 108.6 bits (270), Expect = 2.900e-20
Identity = 74/219 (33.79%), Postives = 103/219 (47.03%), Query Frame = 0
Query:   44 QSLYGTLPFADEKIQ-GPQTIPGKVWLAYFDQGGPDVSYHSYDGKNHGSCELNPCTGTNAIYKNVFRKDEGASTSYTKPPNGGWTGDRFSANNSQVPVDQMYWGWNAIGNWVRLSLLVKKGGNYTLSLFGSSNSGGALTLTVDDYLQKGKAVTTTATIPAT---------DGYHQWALFPDIAVLPSLPPGLALLAVNVTGIYAYKEGSQFGNLLWFDF 253
            QS Y   P+ D + Q GPQ IPG+V+ AY+D GG  ++YH  D KNHGS ELNP  GT   Y + FR  EG  TS+ K        D    + +  P D +Y GW   G W  +++   K   YT     +SN G  +++ V+     GK+ T    I +T           +H W L P++  +  L  G      NV  ++    G    NL +FDF
Sbjct:   11 QSQYKGTPYGDSRYQDGPQKIPGRVFCAYYDLGGEGIAYHDIDEKNHGSGELNPADGT---YLHEFRMHEGVDTSFVKFDRKPDQVDDNPYDRALPPRDMLYVGWTEPGEWFNITVNAAKTETYTADFLYTSNRGATISIDVN-----GKSATAPLGIASTYDSADPVAWRQWHHWNLAPNLFKV-HLDKG-----KNVLTVHILSNGQM--NLGYFDF 213          
BLAST of NO12G00320 vs. NCBI_GenBank
Match: WP_020715406.1 (hypothetical protein [Acidobacteriaceae bacterium KBS 89])

HSP 1 Score: 105.1 bits (261), Expect = 3.200e-19
Identity = 72/205 (35.12%), Postives = 99/205 (48.29%), Query Frame = 0
Query:   41 AVPQSLYGTLPFADEKIQG-PQTIPGKVWLAYFDQGGPDVSYHSYDGKNHGSCELNPCTGTNAIYKNVFRKDEGASTSYTKPPNGGWTGDRFSANNSQV---PVDQMYWGWNAIGNWVRLSLLVKKGGNYTLSLFGSSNSGGALTLTVDDYLQKGKAVTTTATIPATDG---------YHQWALFPDIAVLPSLPPGLALLAVNV 233
            A+    Y   P+ D    G  Q IPG+V  AY+D GG  V+YH  D KN+GS  LNP  GT   Y N FR +EG  TSYTK        D+   N  +V   P + +Y GW   G W ++++ V   G Y+  L  +SN GG + L V+     G++ T   TI +T           +H W   P +  L  LP G  +L V++
Sbjct:   10 ALDHGKYKGTPYHDNHHSGVAQAIPGQVDCAYYDLGGEGVAYHDTDAKNNGSGALNPADGT---YLNQFRMNEGVDTSYTK------FHDQIDDNPYEVIHPPENLLYVGWTEPGEWFKITVEVAHAGIYSADLLYTSNRGGTIALDVN-----GESATLPLTIQSTFNAADPIAWRQWHHWNRAPSLTTL-RLPKGKTVLTVHI 199          
The following BLAST results are available for this feature:
BLAST of NO12G00320 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes)
Total hits: 20
Match NameE-valueIdentityDescription
EWM25981.11.300e-6875.63endoglucanase a [Nannochloropsis gaditana][more]
XP_005854847.15.400e-2768.82hypothetical protein NGA_0367900 [Nannochloropsis ... [more]
WP_051978626.16.200e-2338.34hypothetical protein [Edaphobacter aggregans][more]
OJV44199.12.400e-2236.09hypothetical protein BGO25_10605 [Acidobacteriales... [more]
SPE27393.11.200e-2135.71conserved hypothetical protein [Candidatus Sulfote... [more]
WP_105489074.11.200e-2135.71hypothetical protein [Candidatus Sulfotelmatomonas... [more]
WP_020710045.12.000e-2136.45hypothetical protein [Acidobacteriaceae bacterium ... [more]
WP_011523872.15.800e-2135.50hypothetical protein [Candidatus Koribacter versat... [more]
WP_058186623.12.900e-2033.79hypothetical protein [Terracidiphilus gabretensis][more]
WP_020715406.13.200e-1935.12hypothetical protein [Acidobacteriaceae bacterium ... [more]

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Relationships

This gene is member of the following syntenic_region feature(s):

Feature NameUnique NameSpeciesType
nonsL124nonsL124Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ncniR066ncniR066Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ngnoR006ngnoR006Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region


This gene is orthologous to the following gene feature(s):

Feature NameUnique NameSpeciesType
NSK009920NSK009920Nannochloropsis salina (N. salina CCMP1776)gene


The following polypeptide feature(s) derives from this gene:

Feature NameUnique NameSpeciesType
NO12G00320.1NO12G00320.1-proteinNannochloropsis oceanica (N. oceanica IMET1)polypeptide


The following gene feature(s) are orthologous to this gene:

Feature NameUnique NameSpeciesType
jgi.p|Nanoce1779_2|609691gene_10095Nannochloropsis oceanica (N. oceanica CCMP1779)gene
Naga_100151g12gene4533Nannochloropsis gaditana (N. gaditana B-31)gene


The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
NO12G00320.1NO12G00320.1Nannochloropsis oceanica (N. oceanica IMET1)mRNA


Sequences
The following sequences are available for this feature:

gene sequence

>NO12G00320 ID=NO12G00320|Name=NO12G00320|organism=Nannochloropsis oceanica|type=gene|length=1873bp
ATGGTGATGGCGGTGTTTTCATCACAGGACGAGTTGAAgtagggctatcg
ctatcgcctttggctcgcttgctccctgcatctggctgtggcaataccct
ggctacaccccttcccggaaggatgcacatggactatgggaggggagagg
acaagcgcaactatgcgaagaattatgccggatttatttcgcactagaac
tctaccattaggtccctcactccacagGACTCAACACTACGACATGACGA
TCAGCAGCAGAAGCAGCGCGCGCCCCTTCTTCCTCGTCTTGGCACTGCTA
CCGCTTTTTGCTGTCCCGCAGAGCTTGTATGGGACGCTGCCTTTTGCGGA
CGAGAAGATCCAAGGGCCGCAGgtatatctgggagggatggaagaggttg
gaagtggggaggagcattgtagggcgggagcgtgacaggagggcgagagc
tctatcagagcgggccaggccctcacatcgtatccgcttcacagaactgt
ctcgagcaatttttctgctacaacatcttctggtccaattacccacattc
tcccctccctccctccctcccctccttcctcccctcacagACCATTCCGG
GCAAGGTATGGCTTGCCTACTTCGACCAAGGAGGGCCGGATGTCTCATAT
CATTCGTACGATGGAAAAAACCACGGCTCTTGCGAGCTGAATCCATGCAC
GGGTACCAATGCAATCTATAAAAATGTCTTCCGAAAGgtacagtgaggag
ggtgcagggagggaggagggacaagggagagggaggacggaatgaagtta
aatttaggcgtatcgtatagttggtaaccggtcccggcctattactctcc
ttttttgcgtgccctgagtctttccaacttatcatcgctgctgtacgaca
aatccatctcaccctccattctccatcgtccttcttccctctctcccttt
cccccctcctctacaatacagGACGAAGGCGCCTCAACCTCTTACACCAA
GCCCCCCAACGGCGGATGGACGGGGGACCGCTTTTCCGCCAACAATAGCC
AAGTGCCCGTGGATCAGATGTACTGGGGATGGAATGCGATAGgtgaggcc
gatttctcctcttctccgtctttccctccctcctgccctccctttctccc
tccctcctgccataccttctgttgccctccctccttccctccttccctcc
ctgtcctctccttcccgccctcccttaccccatccctccttttgtcggtg
ggacgtgggccttcctcccccccctccctccctcttgccccccctctgag
cagacctcgcctccctcctccaaccacacttgggaaactactgagccgcc
tttgttcccttcctccttccttccttcccttcttccctccttccctccct
ttcgcagGTAATTGGGTTCGTCTCTCCCTCCTCGTCAAGAAGGGCGGAAA
CTACACCCTCTCCCTTTTTGGTTCCAGCAACAGCGGCGGCGCACTCACAC
TGACGGTAGACGACTACCTGCAGAAGGGCAAGGCGGTGgtaagtgggagg
aatgaagaaatgacccgagggagggagggagggatagagggacgattaat
ttggtgcattttctctctgaatgctctttctcattttttttgagggtgtt
ttctccctcttctcctttcctccctctcctctagACTACCACAGCCACCA
TTCCCGCCACTGACGGGTACCATCAATGGGCACTTTTTCCCGACATCGCC
GTCCTTCCTTCCCTCCCCCCCGGCCTGGCCCTCCTGGCGGTGAATGTGAC
AGGCATTTATGCCTATAAAGAGGGGAGTCAATTCGGGAACTTGCTCTGGT
TCGATTTTCACTGGAAGGGGTAG
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protein sequence of NO12G00320.1

>NO12G00320.1-protein ID=NO12G00320.1-protein|Name=NO12G00320.1|organism=Nannochloropsis oceanica|type=polypeptide|length=257bp
MVMAVFSSQDELKTQHYDMTISSRSSARPFFLVLALLPLFAVPQSLYGTL
PFADEKIQGPQTIPGKVWLAYFDQGGPDVSYHSYDGKNHGSCELNPCTGT
NAIYKNVFRKDEGASTSYTKPPNGGWTGDRFSANNSQVPVDQMYWGWNAI
GNWVRLSLLVKKGGNYTLSLFGSSNSGGALTLTVDDYLQKGKAVTTTATI
PATDGYHQWALFPDIAVLPSLPPGLALLAVNVTGIYAYKEGSQFGNLLWF
DFHWKG*
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Synonyms
Publications