NO11G01250, NO11G01250 (gene) Nannochloropsis oceanica

Overview
NameNO11G01250
Unique NameNO11G01250
Typegene
OrganismNannochloropsis oceanica (N. oceanica IMET1)
Sequence length1277
Alignment locationchr11:438294..439570 +

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Properties
Property NameValue
DescriptionThiamine-phosphate pyrophosphorylase
Mutants
Expression

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
chr11genomechr11:438294..439570 +
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
PRJNA7699582024-08-13
GSE1786722023-12-15
PRJNA9336932023-10-26
PRJNA9434492023-07-19
GSE1499042020-10-10
NoIMET1_WT_HS2020-09-29
GSE1396152020-03-11
PRJNA2413822020-03-11
BLASTP analysis of N. oceanica IMET1 genes2019-07-11
InterPro analysis for N. oceanica IMET1 genes2019-07-11
GO annotation for IMET1v2 genes2019-07-10
PRJNA1821802019-04-25
Gene prediction for N. oceanica IMET12017-10-24
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR036206ThiamineP_synth_sf
IPR022998ThiaminP_synth_SF
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
Homology
BLAST of NO11G01250 vs. NCBI_GenBank
Match: CBJ26257.1 (thiamine-phosphate pyrophosphorylase [Ectocarpus siliculosus])

HSP 1 Score: 98.2 bits (243), Expect = 4.700e-17
Identity = 74/225 (32.89%), Postives = 104/225 (46.22%), Query Frame = 0
Query:   51 PYLVYVTP--AIPAGAEEQKAWFEKVQQCAQAGVPLVQIRDKVLSAEALAPHVTRCREQLDAAAAHYWQPQQQHTHPKTLLIINGHPE--MHIGLDGVHFPEDSLERVSMVLFREXXXXXXXXXXXXXXXXXXXAFVTGVSVHSVEAAVKAAQMGVDYLQVGTMYLTGSHPEKLEVEGTALMTAIDTALREAQLRDRVLICGIGGITPANCEAVLRAGADGVAVI 272
            P L  +TP     +   E  +    +Q+    GV LVQ+RD    A++ A    R    ++  A               L ++NG P+     G DGVH PE  ++R+                            + G SVHSV AAV+AA++G DY+QVGTM+ T SHP K   EG  ++  +   L+   L D VL+ G+GGI  +NC  V+ AG DGVA I
Sbjct:   49 PVLELITPDGCASSSFSETSSLVINIQKAVAGGVSLVQLRDYKSGAKSKADLAVRISTAIEGRA---------------LFVVNGEPDAARASGADGVHLPERMMDRL-----------------VGLRGQGEWPRIVGCSVHSVAAAVEAARLGADYVQVGTMFATQSHPGKTP-EGVGMLNDVRRQLQVEGL-DVVLVLGVGGIDASNCGDVVAAGGDGVAAI 239          
BLAST of NO11G01250 vs. NCBI_GenBank
Match: KPK48294.1 (hypothetical protein AMJ77_00455 [Dehalococcoidia bacterium SM23_28_2])

HSP 1 Score: 91.3 bits (225), Expect = 5.700e-15
Identity = 74/217 (34.10%), Postives = 98/217 (45.16%), Query Frame = 0
Query:   72 EKVQQCAQAGVPLVQIRDKVLSAEALAPHVTRCREQLDAAAAHYWQPQQQHTHPKTLLIINGHPEMHI--GLDGVHFPEDSLERVSMVLFREXXXXXXXXXXXXXXXXXXXAFVTGVSVHSVEAAVKAAQMGVDYLQVGTMYLTGSHPEKLEVEGTALMTAIDTALREAQLRDRVLICGIGGITPANCEAVLRAGADGVAVISGIAAVDNDVPRTVA 287
            E V Q    GV +VQ+R+K L+A  L P   R R                 T  K LLI+N H ++ +    DGVH PE      +M                         F+ G SVHS E AV+A + G DY+QVG ++ T SH   L   G AL+ ++   L        + I  +GGIT  N E V+RAGA+G AVIS I  +D+  P   A
Sbjct:   24 EAVAQAVDGGVNVVQLREKDLTAAQLIPLADRLR---------------AITEDKALLIVNTHLDVALVCAADGVHLPERGPSVAAM------------------RRLAGDGFIIGRSVHSAEEAVRAEEEGADYVQVGAIFPTRSH-RGLPAAGLALLKSVAAML-------AIPIVAVGGITAGNVEQVMRAGAEGAAVISAI--LDSSSPGAAA 197          
BLAST of NO11G01250 vs. NCBI_GenBank
Match: XP_009821548.1 (thiamine-phosphate pyrophosphorylase [Aphanomyces astaci] >ETV89148.1 thiamine-phosphate pyrophosphorylase [Aphanomyces astaci])

HSP 1 Score: 90.9 bits (224), Expect = 7.500e-15
Identity = 59/115 (51.30%), Postives = 73/115 (63.48%), Query Frame = 0
Query:  185 GVSVHSVEAAVKAAQMGVDYLQVGTMYLTGSHPEKLEVEGTALMTAIDTALREAQLRDRVLICGIGGITPANCEAVLRAGADGVAVISGIAAVDNDVPRTVAAFREVFARLDTAS 300
            GVSVH++ +AV AA +   YLQVGTM+ T SHPEK  VEG  LM A+  AL   +L       GIGGIT +NC  V+RAGA+G+AVI  I  +D D P  +AA R +   LD AS
Sbjct:  118 GVSVHAISSAVTAAALESSYLQVGTMFPTASHPEKTSVEGPELMRAVRQALPSTRL------IGIGGITESNCVDVMRAGANGIAVIRAI--LDADKP-DIAATR-LRQALDNAS 222          
BLAST of NO11G01250 vs. NCBI_GenBank
Match: XP_008862568.1 (thiamine-phosphate pyrophosphorylase [Aphanomyces invadans] >ETW08763.1 thiamine-phosphate pyrophosphorylase [Aphanomyces invadans])

HSP 1 Score: 90.1 bits (222), Expect = 1.300e-14
Identity = 67/170 (39.41%), Postives = 85/170 (50.00%), Query Frame = 0
Query:  120 QQHTHPKTLLIIN---GHPEMHIGLDGVHFPEDSLERVSMVLFREXXXXXXXXXXXXXXXXXXXAFVTGVSVHSVEAAVKAAQMGVDYLQVGTMYLTGSHPEKLEVEGTALMTAIDTALREAQLRDRVLICGIGGITPANCEAVLRAGADGVAVISGIAAVDNDVPRTVA 287
            +Q T P  L ++N   G P+    LDG H+PE  L  +  +L +                        GVSVH+  +A  AA +G  Y+QVGTM+ T SHPEK  VEG  L+  +  AL  A L       G+GGIT ANC  VL+AGA GVAVI  I  +D D P   A
Sbjct:   67 EQLTKP-PLYVVNTPIGLPDSTPRLDGWHYPERCLSTLEGMLEQHGRGFF------------------GVSVHAAASAETAAALGASYIQVGTMFPTASHPEKTCVEGPELIRQVRQALPSATL------IGVGGITDANCVDVLQAGASGVAVIRAI--LDADKPDVAA 209          
BLAST of NO11G01250 vs. NCBI_GenBank
Match: WP_024327492.1 (thiamine phosphate synthase [Thioalkalivibrio sp. AKL19])

HSP 1 Score: 83.2 bits (204), Expect = 1.600e-12
Identity = 78/247 (31.58%), Postives = 108/247 (43.72%), Query Frame = 0
Query:   53 LVYVTPAIPAGAEEQKAWFEKVQQCAQAGVPLVQIRDKVLSAEALAPHVTRCREQLDAAAAHYWQPQQQHTHPKTLLIINGHPEM--HIGLDGVHFPEDSLERVSMVLFREXXXXXXXXXXXXXXXXXXXAFVTGVSVHSVEAAVKAA-QMGVDYLQVGTMYLTGSHPEKLEVEGTALMTAIDTALREAQLRDRVLICGIGGITPANCEAVLRAGADGVAVISGIAAVDNDVPRTVAAFREVFARLD 297
            L +VTPA+P GA+ +    E  +   + G  L+Q RDKVL+ +A        RE +  A AH             L IIN   E+   +G DGVH   D  + V+                           V GVS ++  A  +AA + G +Y+  GTM+ + + PE           A    +R A+    + ICGIGGIT  N   V+ AGAD VAVI GI+A     P   AA R++    D
Sbjct:   12 LYFVTPAVPEGADPRTTHLENSRAALRGGARLIQFRDKVLANDA---REAIARELVALAHAH-----------GALCIINDDTELAARVGADGVHLGRDDPDPVA------------------ARARLGPGAVVGVSCYNELARAEAATRTGANYVAFGTMFASPTKPE--------AAYAGPELIRRARAALDLPICGIGGITADNAAEVVTAGADLVAVIQGISA----APDPEAAARQISELFD 214          
BLAST of NO11G01250 vs. NCBI_GenBank
Match: OGO48492.1 (hypothetical protein A2W34_04135 [Chloroflexi bacterium RBG_16_64_32])

HSP 1 Score: 83.2 bits (204), Expect = 1.600e-12
Identity = 73/233 (31.33%), Postives = 100/233 (42.92%), Query Frame = 0
Query:   46 LSVPTPYLVYVTPAIPAGAEEQKAWFEKVQQCAQAGVPLVQIRDKVLSAEALAPHVTRCREQLDAAAAHYWQPQQQHTHPKTLLIINGHPEM--HIGLDGVHFPEDSLERVSMVLFREXXXXXXXXXXXXXXXXXXXAFVTGVSVHSVEAAVKAAQMGVDYLQVGTMYLTGSHPEKLEVEGTALMTAIDTALREAQLRDRVLICGIGGITPANCEAVLRAGADGVAVISGIAA 277
            + +P P L+ VT     G  +  A  + V+   + G   VQ+R+K L A  L+    R RE    A                LL++NG  E+    G DGVH PED+                               F+ G S+HS  AA +A   G DYL  G +Y T SHP + E  G AL+  +   +       R+ +  IGG+T    E V+RAGA GVAVIS + A
Sbjct:    1 MPLPRPCLMLVTDRSLCGGADGLA--DAVEAAVEGGADAVQLREKSLPASELSTLARRLREVTRGA----------------LLVVNGPLEVATEAGADGVHLPEDA----------------------PPVRRPRQGFLVGRSIHSPVAARRAEAEGADYLVAGPVYETRSHPGR-EPAGLALIEQVTQIV-------RIPVLAIGGVTAGRVEEVVRAGASGVAVISAVLA 185          
BLAST of NO11G01250 vs. NCBI_GenBank
Match: GBD11024.1 (Thiazole tautomerase [bacterium HR23])

HSP 1 Score: 82.8 bits (203), Expect = 2.000e-12
Identity = 80/265 (30.19%), Postives = 110/265 (41.51%), Query Frame = 0
Query:   46 LSVPTPYLVYVTP--AIPAGAEEQKAWFEKVQQCAQAGVPLVQIRDKVLSAEALAPHVTRCREQLDAAAAHYWQPQQQHTHPKTLLIINGHPEMHI--GLDGVHFPEDSLERVSMVLFREXXXXXXXXXXXXXXXXXXXAFVTGVSVHSVEAAVKAAQMGVDYLQVGTMYLTGSHPEKLEVEGTALMTAIDTALREAQLRDRVLICGIGGITPANCEAVLRAGADGVAVISGIAAVDNDVPRTVAAFREVFARLDTASKNRGDAC 307
            + +P P L  VT     P+    Q A  E V Q  + GV LVQ+R+K L    L     R RE                T  + LL IN   ++ +    DGVH PE  L   ++                          + G SVHSVEA  +A Q G D L  GT++ + SHP +    G  L+TA+  A+        + +  IGG+TP N    +RAGA GVAV++ I       PR  A+ R +   +  A    G  C
Sbjct:    1 MPIPLPCLALVTDRHLYPS----QDALVEAVAQAVEGGVDLVQVREKDLPGGHLLALAVRLREV---------------TRGRALLFINERADVALLSEADGVHLPEAGLPPSAV------------------RRLAGRRLLIGASVHSVEAGYRAEQEGADLLVAGTIFPSRSHPGQ-SATGVGLLTALRKAV-------SLPVLAIGGVTPQNAGECIRAGAHGVAVVTAILG----QPRPAASARRLREAMRQAWAGGGALC 216          
BLAST of NO11G01250 vs. NCBI_GenBank
Match: XP_008608177.1 (hypothetical protein SDRG_04288 [Saprolegnia diclina VS20] >EQC38585.1 hypothetical protein SDRG_04288 [Saprolegnia diclina VS20])

HSP 1 Score: 82.4 bits (202), Expect = 2.700e-12
Identity = 57/122 (46.72%), Postives = 67/122 (54.92%), Query Frame = 0
Query:  183 VTGVSVHSVEAAVKAAQMGVDYLQVGTMYLTGSHPEKLEVEGTALMTAIDTALREAQLRDRVLICGIGGITPANCEAVLRAGADGVAVISGIAAVDNDVPRTVAAFREVFARLDTASKNRGD 305
            + G SVHSV +A +A ++G  YLQVGTM+ T SHPEK  VEG AL+  I            V + GIGGI   NC  VL AGA GV+VI  I A     P TVA    +   LDT    RGD
Sbjct:  117 IYGFSVHSVPSARRAIKLGASYLQVGTMFPTASHPEKKVVEGPALIHEIHKRF------PNVPLIGIGGIDETNCAEVLAAGASGVSVIRAILAAKG--PSTVA--EHLRRELDT---RRGD 225          
BLAST of NO11G01250 vs. NCBI_GenBank
Match: WP_028491377.1 (thiamine phosphate synthase [Thioalkalivibrio sp. ALE19])

HSP 1 Score: 79.0 bits (193), Expect = 2.900e-11
Identity = 83/261 (31.80%), Postives = 107/261 (41.00%), Query Frame = 0
Query:   41 TTPPKLSVPTPYLVYVTPAIPAGAEEQKAWFEKVQQCAQAGVPLVQIRDKVLSAEALAPHVTRCREQLDAAAAHYWQPQQQHTHPKTLLIINGHPEM--HIGLDGVHFPEDSLERVSMVLFREXXXXXXXXXXXXXXXXXXXAFVTGVSVHSVEAAVKAAQ-MGVDYLQVGTMYLTGSHPEKLEVEGTALMTAIDTALREAQLRDRVLICGIGGITPANCEAVLRAGADGVAVISGIAAVDNDVPRTVAAFREVFARLDTA 299
            T P     P   L +VTPA P GA+   A  +        G   VQ+RDK L  EA        +E +  A A        H H     I+N   E+   IG DGVH   D  +                            A V GVS ++  A  +AAQ  G DY+  G+M+ + + PE +         A    +R+A+    V IC IGGIT  N   V+ AGAD VAVI GI+A     P   AA R + A   TA
Sbjct:    2 TEPVTERAPLRGLYFVTPAPPPGADPLPAHTDAAAAALAGGACCVQLRDKYLEGEA--------QETVARALARLC-----HDH-GAYFIVNDDVELAARIGADGVHLGRDDPDPAG------------------ARARLGDAAVIGVSCYNELARARAAQAAGADYVAFGSMFPSPTKPEAVR--------ATPELIRQARRELAVAICAIGGITADNAAQVVAAGADMVAVIQGISA----APDPEAAARRIAALFRTA 218          
BLAST of NO11G01250 vs. NCBI_GenBank
Match: XP_005539074.1 (similar to thiamine phosphate pyrophosphorylase [Cyanidioschyzon merolae strain 10D] >BAM83038.1 similar to thiamine phosphate pyrophosphorylase [Cyanidioschyzon merolae strain 10D])

HSP 1 Score: 78.6 bits (192), Expect = 3.800e-11
Identity = 51/99 (51.52%), Postives = 58/99 (58.59%), Query Frame = 0
Query:  183 VTGVSVHSVEAAVKAAQMG-VDYLQVGTMYLTGSHPEKLE---VEGTALMTAIDTALREAQLRDRVLICGIGGITPANCEAVLRAGADGVAVISGIAAV 278
            V G SVHSV  A+ AA+ G V YL VGTM+ T SHPEK     +EG  LM A+  AL  + L     + GIGGITP N   VL AGA GVA I  I  V
Sbjct:  131 VLGTSVHSVSEALFAAETGLVSYLLVGTMFATASHPEKSSPEFIEGIPLMRAVSQALSTSSLH----LYGIGGITPGNINQVLEAGAHGVATIRSIVPV 225          
The following BLAST results are available for this feature:
BLAST of NO11G01250 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes)
Total hits: 20
Match NameE-valueIdentityDescription
CBJ26257.14.700e-1732.89thiamine-phosphate pyrophosphorylase [Ectocarpus s... [more]
KPK48294.15.700e-1534.10hypothetical protein AMJ77_00455 [Dehalococcoidia ... [more]
XP_009821548.17.500e-1551.30thiamine-phosphate pyrophosphorylase [Aphanomyces ... [more]
XP_008862568.11.300e-1439.41thiamine-phosphate pyrophosphorylase [Aphanomyces ... [more]
WP_024327492.11.600e-1231.58thiamine phosphate synthase [Thioalkalivibrio sp. ... [more]
OGO48492.11.600e-1231.33hypothetical protein A2W34_04135 [Chloroflexi bact... [more]
GBD11024.12.000e-1230.19Thiazole tautomerase [bacterium HR23][more]
XP_008608177.12.700e-1246.72hypothetical protein SDRG_04288 [Saprolegnia dicli... [more]
WP_028491377.12.900e-1131.80thiamine phosphate synthase [Thioalkalivibrio sp. ... [more]
XP_005539074.13.800e-1151.52similar to thiamine phosphate pyrophosphorylase [C... [more]

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Relationships

This gene is member of the following syntenic_region feature(s):

Feature NameUnique NameSpeciesType
nonsL116nonsL116Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
nonsL115nonsL115Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ncniR010ncniR010Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ngnoR019ngnoR019Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ngnoR018ngnoR018Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region


This gene is orthologous to the following gene feature(s):

Feature NameUnique NameSpeciesType
NSK002144NSK002144Nannochloropsis salina (N. salina CCMP1776)gene


The following polypeptide feature(s) derives from this gene:

Feature NameUnique NameSpeciesType
NO11G01250.1NO11G01250.1-proteinNannochloropsis oceanica (N. oceanica IMET1)polypeptide


The following gene feature(s) are orthologous to this gene:

Feature NameUnique NameSpeciesType
jgi.p|Nanoce1779_2|591072gene_5006Nannochloropsis oceanica (N. oceanica CCMP1779)gene


The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
NO11G01250.1NO11G01250.1Nannochloropsis oceanica (N. oceanica IMET1)mRNA


Sequences
The following sequences are available for this feature:

gene sequence

>NO11G01250 ID=NO11G01250|Name=NO11G01250|organism=Nannochloropsis oceanica|type=gene|length=1277bp
ATGGGAAGAAATAGGGTGCCACCGATACCTTTACTGATCACCGCGGTGCC
ACTGACGAGTCTAACCCTTATCGTCTGGATTATTAGTGTCGTCGCTTTAA
GACAGCAGTTACCCCCCTCAACCACTCCTCCAAAGCTGTCTGTCCCCACG
CCCTACCTCGTCTACGTAACTCCGGCCATTCCTGCAGGGGCGGAGGAGCA
GAAGGCTTGGTTCGAGAAGgtaattttagcgaaatgtgggctaacaagcc
ttgaatcttcctcactcttccccctcgacaactccacataagGTTCAGCA
ATGCGCCCAGGCCGGAGTGCCATTAGTGCAAATCCGCGACAAGGTTTTGT
CTGCAGAGgtaagaaatggaattcaaggtgaatttgcctctgacatctgc
acatactctgttcattacctcctttctctccacccattccactcaagGCC
CTGGCACCCCATGTGACGCGATGCCGGGAGCAACTTGATGCTGCCGCAGC
CCACTACTGGCAGCCGCAACAGCAGCATACGCACCCCAAAACCCTTCTTA
TTATCAATGGTCATCCTGAAATGCATATAGGGTTGGATGGAGTCCATTTC
CCTGAGGACAGTCTGGAGCGCGTATCCATGGTGCTTTTTAGAGAGCAAAA
GCGGCAGGAGGAGAAGGAGGAGGAGGAAGAAGAAAAGCAGCGGCAGCAGC
TAGCCTTTGTGACGGGCGTGTCGGTGCATAGCGTGgtgtgtatccgagaa
gcggtggcatacgtttacgttctctatacctgacgatgtcgtttcatgca
tacatatagGAAGCAGCTGTCAAGGCCGCACAGATGGGGGTCGACTACTT
GCAGgttcgctgcgaaagaagggaaaattagaccgtccccgccgccgata
agatgtcttactttatctccaactacatgcatacatgcattcagGTGGGC
ACCATGTACCTGACGGGGAGCCACCCCGAAAAGCTGGAGGTGGAAGGCAC
GGCCTTAATGACGGCCATAGACACGGCGCTCCGCGAAGCCCAGCTTCGGG
ACCGCGTTCTTATCTGTGGTATTGGGGGCATCACCCCCGCCAATTGTGAG
GCTGTGCTACGGGCAGGTGCAGATGGAGTGGCGGTCATCAGTGGCATTGC
AGCGGTGGACAATGATGTGCCCCGTACCGTGGCTGCGTTTCGCGAGGTGT
TTGCGAGGCTAGATACGGCATCAAAAAACAGAGGAGATGCATGTCTATAG
GGTCAGAGAATGTATTAAACGAAATTC
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protein sequence of NO11G01250.1

>NO11G01250.1-protein ID=NO11G01250.1-protein|Name=NO11G01250.1|organism=Nannochloropsis oceanica|type=polypeptide|length=308bp
MGRNRVPPIPLLITAVPLTSLTLIVWIISVVALRQQLPPSTTPPKLSVPT
PYLVYVTPAIPAGAEEQKAWFEKVQQCAQAGVPLVQIRDKVLSAEALAPH
VTRCREQLDAAAAHYWQPQQQHTHPKTLLIINGHPEMHIGLDGVHFPEDS
LERVSMVLFREQKRQEEKEEEEEEKQRQQLAFVTGVSVHSVEAAVKAAQM
GVDYLQVGTMYLTGSHPEKLEVEGTALMTAIDTALREAQLRDRVLICGIG
GITPANCEAVLRAGADGVAVISGIAAVDNDVPRTVAAFREVFARLDTASK
NRGDACL*
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Synonyms
Publications