NO08G00480, NO08G00480 (gene) Nannochloropsis oceanica

Overview
NameNO08G00480
Unique NameNO08G00480
Typegene
OrganismNannochloropsis oceanica (N. oceanica IMET1)
Sequence length1130
Alignment locationchr8:187681..188810 -

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Properties
Property NameValue
DescriptionDna-directed rna polymerases and iii subunit rpabc5
Mutants
Expression

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
chr8genomechr8:187681..188810 -
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
PRJNA7699582024-08-13
GSE1786722023-12-15
PRJNA9336932023-10-26
PRJNA9434492023-07-19
PXD0160542021-01-14
GSE1499042020-10-10
NoIMET1_WT_HS2020-09-29
GSE1396152020-03-11
PRJNA2413822020-03-11
BLASTP analysis of N. oceanica IMET1 genes2019-07-11
InterPro analysis for N. oceanica IMET1 genes2019-07-11
GO annotation for IMET1v2 genes2019-07-10
PRJNA1821802019-04-25
Gene prediction for N. oceanica IMET12017-10-24
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0003899DNA-directed 5'-3' RNA polymerase activity
GO:0003677DNA binding
GO:0016740transferase activity
GO:0003677DNA binding
Vocabulary: Biological Process
TermDefinition
GO:0006351transcription, DNA-templated
GO:0009058biosynthetic process
GO:0006139nucleobase-containing compound metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR023580RNA_pol_su_RPB10
IPR000268RNAP_N/Rpb10
Homology
BLAST of NO08G00480 vs. NCBI_GenBank
Match: EWM26536.1 (dna-directed rna polymerases and iii subunit rpabc5 [Nannochloropsis gaditana])

HSP 1 Score: 154.1 bits (388), Expect = 1.800e-34
Identity = 70/75 (93.33%), Postives = 75/75 (100.00%), Query Frame = 0
Query:    1 MIIPVRCFTCGKVIGNKYEVYVEYLKNDYKEGVALDKVGLKRYCCRRMVLTHVDLISKLLNYNTLAKSGYLKDGE 76
            MIIPVRCFTCGKVIGNKYEVYVEYLKND+KEGVALDKVGLKRYCCRRM+LTHVDLISKLLNYNTLAK+GY+K+GE
Sbjct:   39 MIIPVRCFTCGKVIGNKYEVYVEYLKNDFKEGVALDKVGLKRYCCRRMLLTHVDLISKLLNYNTLAKAGYVKEGE 113          
BLAST of NO08G00480 vs. NCBI_GenBank
Match: XP_009041410.1 (hypothetical protein AURANDRAFT_33434 [Aureococcus anophagefferens] >EGB03858.1 hypothetical protein AURANDRAFT_33434 [Aureococcus anophagefferens])

HSP 1 Score: 119.4 bits (298), Expect = 4.900e-24
Identity = 55/67 (82.09%), Postives = 60/67 (89.55%), Query Frame = 0
Query:    1 MIIPVRCFTCGKVIGNKYEVYVEYLKNDYKEGVALDKVGLKRYCCRRMVLTHVDLISKLLNYNTLAK 68
            MIIPVRCFTCGKVIGNK+E Y+E LK DY EG ALD++GLKRYCCRRMVLTHVDLI KLLNYNTL +
Sbjct:    1 MIIPVRCFTCGKVIGNKWESYLELLKADYSEGDALDELGLKRYCCRRMVLTHVDLIEKLLNYNTLER 67          
BLAST of NO08G00480 vs. NCBI_GenBank
Match: XP_003517695.1 (PREDICTED: DNA-directed RNA polymerase subunit 10-like protein [Glycine max] >XP_003520009.1 PREDICTED: DNA-directed RNA polymerase subunit 10-like protein [Glycine max] >ACU16037.1 unknown [Glycine max] >KHN14567.1 DNA-directed RNA polymerases I, II, and III subunit RPABC5 [Glycine soja] >KRH69587.1 hypothetical protein GLYMA_02G035900 [Glycine max])

HSP 1 Score: 114.8 bits (286), Expect = 1.200e-22
Identity = 51/68 (75.00%), Postives = 60/68 (88.24%), Query Frame = 0
Query:    1 MIIPVRCFTCGKVIGNKYEVYVEYLKNDYKEGVALDKVGLKRYCCRRMVLTHVDLISKLLNYNTLAKS 69
            MIIPVRCFTCGKVIGNK++ Y++ L++DY EG ALD +GL RYCCRRM++THVDLI KLLNYNTL KS
Sbjct:    1 MIIPVRCFTCGKVIGNKWDTYLDLLQSDYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKS 68          
BLAST of NO08G00480 vs. NCBI_GenBank
Match: XP_022034621.1 (DNA-directed RNA polymerases I, II, and III subunit RPABC5 [Helianthus annuus] >XP_023759216.1 DNA-directed RNA polymerases I, II, and III subunit RPABC5 [Lactuca sativa] >XP_023737604.1 DNA-directed RNA polymerases I, II, and III subunit RPABC5 [Lactuca sativa] >KVH94217.1 DNA-directed RNA polymerase, subunit N/Rpb10 [Cynara cardunculus var. scolymus] >KVI12261.1 DNA-directed RNA polymerase, subunit N/Rpb10 [Cynara cardunculus var. scolymus] >OTG28159.1 putative DNA-directed RNA polymerase, subunit N/Rpb10 [Helianthus annuus])

HSP 1 Score: 114.8 bits (286), Expect = 1.200e-22
Identity = 51/68 (75.00%), Postives = 60/68 (88.24%), Query Frame = 0
Query:    1 MIIPVRCFTCGKVIGNKYEVYVEYLKNDYKEGVALDKVGLKRYCCRRMVLTHVDLISKLLNYNTLAKS 69
            MIIPVRCFTCGKVIGNK++ Y++ L++DY EG ALD +GL RYCCRRM++THVDLI KLLNYNTL KS
Sbjct:    1 MIIPVRCFTCGKVIGNKWDTYLDLLQSDYTEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLEKS 68          
BLAST of NO08G00480 vs. NCBI_GenBank
Match: XP_015953583.1 (DNA-directed RNA polymerase subunit 10-like protein [Arachis duranensis] >XP_015953584.1 DNA-directed RNA polymerase subunit 10-like protein [Arachis duranensis] >XP_015953585.1 DNA-directed RNA polymerase subunit 10-like protein [Arachis duranensis] >XP_015965053.1 DNA-directed RNA polymerase subunit 10-like protein [Arachis duranensis] >XP_016188698.1 DNA-directed RNA polymerase subunit 10-like protein [Arachis ipaensis] >XP_016188699.1 DNA-directed RNA polymerase subunit 10-like protein [Arachis ipaensis] >XP_016188700.1 DNA-directed RNA polymerase subunit 10-like protein [Arachis ipaensis])

HSP 1 Score: 114.8 bits (286), Expect = 1.200e-22
Identity = 51/68 (75.00%), Postives = 60/68 (88.24%), Query Frame = 0
Query:    1 MIIPVRCFTCGKVIGNKYEVYVEYLKNDYKEGVALDKVGLKRYCCRRMVLTHVDLISKLLNYNTLAKS 69
            MIIPVRCFTCGKVIGNK++ Y++ L++DY EG ALD +GL RYCCRRM++THVDLI KLLNYNTL KS
Sbjct:    1 MIIPVRCFTCGKVIGNKWDAYLDLLQSDYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKS 68          
BLAST of NO08G00480 vs. NCBI_GenBank
Match: XP_016200727.1 (DNA-directed RNA polymerase subunit 10-like protein [Arachis ipaensis])

HSP 1 Score: 114.8 bits (286), Expect = 1.200e-22
Identity = 51/68 (75.00%), Postives = 60/68 (88.24%), Query Frame = 0
Query:    1 MIIPVRCFTCGKVIGNKYEVYVEYLKNDYKEGVALDKVGLKRYCCRRMVLTHVDLISKLLNYNTLAKS 69
            MIIPVRCFTCGKVIGNK++ Y++ L++DY EG ALD +GL RYCCRRM++THVDLI KLLNYNTL KS
Sbjct:   44 MIIPVRCFTCGKVIGNKWDAYLDLLQSDYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKS 111          
BLAST of NO08G00480 vs. NCBI_GenBank
Match: XP_022010075.1 (DNA-directed RNA polymerase subunit 10-like protein [Helianthus annuus] >OTF98439.1 putative RNA polymerases N / 8 kDa subunit [Helianthus annuus])

HSP 1 Score: 114.8 bits (286), Expect = 1.200e-22
Identity = 51/68 (75.00%), Postives = 60/68 (88.24%), Query Frame = 0
Query:    1 MIIPVRCFTCGKVIGNKYEVYVEYLKNDYKEGVALDKVGLKRYCCRRMVLTHVDLISKLLNYNTLAKS 69
            MIIPVRCFTCGKVIGNK++ Y++ L++DY EG ALD +GL RYCCRRM++THVDLI KLLNYNTL KS
Sbjct:    1 MIIPVRCFTCGKVIGNKWDTYLDLLQSDYTEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLEKS 68          
BLAST of NO08G00480 vs. NCBI_GenBank
Match: KFK40670.1 (hypothetical protein AALP_AA2G026200 [Arabis alpina])

HSP 1 Score: 114.4 bits (285), Expect = 1.600e-22
Identity = 51/68 (75.00%), Postives = 59/68 (86.76%), Query Frame = 0
Query:    1 MIIPVRCFTCGKVIGNKYEVYVEYLKNDYKEGVALDKVGLKRYCCRRMVLTHVDLISKLLNYNTLAKS 69
            MIIPVRCFTCGKVIGNK++ Y+E L+ DY EG ALD +GL RYCCRRM++THVDLI KLLNYNT+ KS
Sbjct:    1 MIIPVRCFTCGKVIGNKWDTYLELLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTMEKS 68          
BLAST of NO08G00480 vs. NCBI_GenBank
Match: XP_009105127.1 (PREDICTED: DNA-directed RNA polymerase subunit 10-like protein [Brassica rapa] >XP_013591461.1 PREDICTED: DNA-directed RNA polymerase subunit 10-like protein [Brassica oleracea var. oleracea] >XP_013670204.1 DNA-directed RNA polymerase subunit 10-like protein [Brassica napus] >XP_013670232.1 DNA-directed RNA polymerase subunit 10-like protein [Brassica napus] >XP_018437442.1 PREDICTED: DNA-directed RNA polymerase subunit 10-like protein [Raphanus sativus] >CDY52320.1 BnaA01g36190D [Brassica napus])

HSP 1 Score: 114.4 bits (285), Expect = 1.600e-22
Identity = 51/68 (75.00%), Postives = 59/68 (86.76%), Query Frame = 0
Query:    1 MIIPVRCFTCGKVIGNKYEVYVEYLKNDYKEGVALDKVGLKRYCCRRMVLTHVDLISKLLNYNTLAKS 69
            MIIPVRCFTCGKVIGNK++ Y++ L+ DY EG ALD +GL RYCCRRM++THVDLI KLLNYNTL KS
Sbjct:    1 MIIPVRCFTCGKVIGNKWDTYLDLLQADYTEGDALDAIGLVRYCCRRMLMTHVDLIEKLLNYNTLEKS 68          
BLAST of NO08G00480 vs. NCBI_GenBank
Match: XP_017250243.1 (PREDICTED: DNA-directed RNA polymerase subunit 10-like protein [Daucus carota subsp. sativus] >KZM95396.1 hypothetical protein DCAR_018638 [Daucus carota subsp. sativus])

HSP 1 Score: 114.4 bits (285), Expect = 1.600e-22
Identity = 51/68 (75.00%), Postives = 60/68 (88.24%), Query Frame = 0
Query:    1 MIIPVRCFTCGKVIGNKYEVYVEYLKNDYKEGVALDKVGLKRYCCRRMVLTHVDLISKLLNYNTLAKS 69
            MIIPVRCFTCGKVIGNK++ Y++ L+ DY+EG ALD +GL RYCCRRM++THVDLI KLLNYNTL KS
Sbjct:    1 MIIPVRCFTCGKVIGNKWDTYLDLLQADYEEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLEKS 68          
The following BLAST results are available for this feature:
BLAST of NO08G00480 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes)
Total hits: 20
Match NameE-valueIdentityDescription
EWM26536.11.800e-3493.33dna-directed rna polymerases and iii subunit rpabc... [more]
XP_009041410.14.900e-2482.09hypothetical protein AURANDRAFT_33434 [Aureococcus... [more]
XP_003517695.11.200e-2275.00PREDICTED: DNA-directed RNA polymerase subunit 10-... [more]
XP_022034621.11.200e-2275.00DNA-directed RNA polymerases I, II, and III subuni... [more]
XP_015953583.11.200e-2275.00DNA-directed RNA polymerase subunit 10-like protei... [more]
XP_016200727.11.200e-2275.00DNA-directed RNA polymerase subunit 10-like protei... [more]
XP_022010075.11.200e-2275.00DNA-directed RNA polymerase subunit 10-like protei... [more]
KFK40670.11.600e-2275.00hypothetical protein AALP_AA2G026200 [Arabis alpin... [more]
XP_009105127.11.600e-2275.00PREDICTED: DNA-directed RNA polymerase subunit 10-... [more]
XP_017250243.11.600e-2275.00PREDICTED: DNA-directed RNA polymerase subunit 10-... [more]

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Relationships

This gene is member of the following syntenic_region feature(s):

Feature NameUnique NameSpeciesType
nonsL109nonsL109Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ncniR083ncniR083Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ngnoR141ngnoR141Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region


This gene is orthologous to the following gene feature(s):

Feature NameUnique NameSpeciesType
NSK009020NSK009020Nannochloropsis salina (N. salina CCMP1776)gene


The following polypeptide feature(s) derives from this gene:

Feature NameUnique NameSpeciesType
NO08G00480.1NO08G00480.1-proteinNannochloropsis oceanica (N. oceanica IMET1)polypeptide


The following gene feature(s) are orthologous to this gene:

Feature NameUnique NameSpeciesType
Naga_100141g12gene3994Nannochloropsis gaditana (N. gaditana B-31)gene


The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
NO08G00480.1NO08G00480.1Nannochloropsis oceanica (N. oceanica IMET1)mRNA


Sequences
The following sequences are available for this feature:

gene sequence

>NO08G00480 ID=NO08G00480|Name=NO08G00480|organism=Nannochloropsis oceanica|type=gene|length=1130bp
ACAGGACGGAGAGAAAAGGGTCGTTCAAGATAGCTGAGGAATTAACAAGA
AGAGGGGGGTCGTCGCAAGTTCACCTAGAGCACTATGATTATTCCTGTGC
GCTGCTTCACGTGTGGCAAGgtaaacagagaatatgaggaggaggcaagg
gagggtgtggaagcgccttgggagatatgggggcgagacggcaaagagga
ggttcaaatgaggaggaattaaggcatggggcacgaagcggacaagaaga
gtgtcacgagaacatgaacggtagacctcctgaactatgctgcgtgagaa
gacgcgatgaaagtctatgacttacacctttcttcttgtcccctccctcc
ctttccaactccaccagGTCATCGGCAACAAGTACGAAGTGTACGTGGAG
TACCTCAAGAATGACTACAAAGAGGGgtacgtgtctgaagcgaagatggg
tagtgaatagatgagttaagaaaagtggcgtgctttgccttagttttgtc
acttgggttcaccccttgttgtctttgaacacctgatacgcctgttgcgc
tctccctcacaagtacttttccttcccgtgacattttttgccggagtaga
aagctgtaggatcattactatgtgtgatgttgtcaggcacgacactcggg
tgatggaattctttgtatctttccttcttgccctctctcatctccctctc
tattactgtacaagtcaaatggttcaggctcaatgcaaccatgtccctcc
ttgtcgctgctcttatctcacaaaccttttgtccttctcaccttaccttt
tcaccctgtaacacagTGTGGCGCTGGACAAGGTGGGCTTGAAGCGGTAC
TGCTGCCGACGGATGGTTCTCACGCACGTGGACCTGATTAGCAAGCTTCT
TAACTACAATACCTTGGCAAAATCTGGCTACCTTAAGGACGGGGAGTAGT
AATATATCCTTTGCTCAATCAAGACAATGAATGATGAAGGTTTGAAGTGC
ACATTTCCAGGGGGCGGGAGGGAGTTATGGAGGCCAGGACGGGAGGGAAA
ATATCGCGCAGAGGCCTAGTTAAGACAAGTGACCCTAAACCCTACATGTA
CAAAGAAACCAGACACAGATTAAGTTGAAA
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protein sequence of NO08G00480.1

>NO08G00480.1-protein ID=NO08G00480.1-protein|Name=NO08G00480.1|organism=Nannochloropsis oceanica|type=polypeptide|length=76bp
MIIPVRCFTCGKVIGNKYEVYVEYLKNDYKEGVALDKVGLKRYCCRRMVL
THVDLISKLLNYNTLAKSGYLKDGE*
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Synonyms
Publications