NO06G04150, NO06G04150 (gene) Nannochloropsis oceanica

Overview
NameNO06G04150
Unique NameNO06G04150
Typegene
OrganismNannochloropsis oceanica (N. oceanica IMET1)
Sequence length1192
Alignment locationchr6:1158207..1159398 -

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Properties
Property NameValue
DescriptionRibosomal large subunit pseudouridine synthase B
Mutants
Expression

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
chr6genomechr6:1158207..1159398 -
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
PRJNA7699582024-08-13
GSE1786722023-12-15
PRJNA9336932023-10-26
PRJNA9434492023-07-19
GSE1499042020-10-10
NoIMET1_WT_HS2020-09-29
GSE1396152020-03-11
PRJNA2413822020-03-11
BLASTP analysis of N. oceanica IMET1 genes2019-07-11
InterPro analysis for N. oceanica IMET1 genes2019-07-11
GO annotation for IMET1v2 genes2019-07-10
PRJNA1821802019-04-25
Gene prediction for N. oceanica IMET12017-10-24
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0016866intramolecular transferase activity
GO:0009982pseudouridine synthase activity
GO:0003723RNA binding
GO:0016866intramolecular transferase activity
GO:0009982pseudouridine synthase activity
GO:0003723RNA binding
Vocabulary: Biological Process
TermDefinition
GO:0009451RNA modification
GO:0001522pseudouridine synthesis
GO:0009451RNA modification
GO:0001522pseudouridine synthesis
Vocabulary: INTERPRO
TermDefinition
IPR020103PsdUridine_synth_cat_dom
IPR000748PsdUridine_synth_RsuA/RluB/E/F
IPR006145PsdUridine_synth_RsuA/RluD
IPR002942S4_RNA-bd
Homology
[Close]
BLAST of NO06G04150 vs. NCBI_GenBank
Match: CEM01544.1 (unnamed protein product [Vitrella brassicaformis CCMP3155])

HSP 1 Score: 167.5 bits (423), Expect = 7.700e-38
Identity = 93/196 (47.45%), Postives = 131/196 (66.84%), Query Frame = 0 
Query:  169 ARKGQAGTSRKKSSPSSPSIAPKGSVVSLSPRGATTRLNKMLAHAGVASRRSAEEMIAQGKVSVNGVIVLDVGCKVDPSVDSITVEGKAAPVALRKASEVVWMKVNKPKGTLTSITDSKGRKCLGDLVESAVEKRLLPVGRLDRDTAGLLLMTNDNEAINLLTHPSFGHVKLYKAVVEKGMPSVHVLHQLKRGLVL 365
            A K  + +S +  S ++PS+       SL  R A  RL K+++ AG+ASRR+AEEMI  G+V VNGV+V D+G KVD   D +TV+G+   +A+R   E+ W+ V+KPKG + S+ D + RK + DLV  A   RLLPVGRLDRD++GLLL+TNDN+ ++ LTHPS+GH K Y   V+ G P   VL++L  G++L
Sbjct:  108 AAKLDSSSSLQDPSQNAPSLP------SLEERSAPERLQKVISRAGIASRRAAEEMILDGRVRVNGVVVTDLGVKVDALGDRVTVDGR--KIAVRPPEEIKWIAVHKPKGYICSMNDDRDRKTVKDLVPMAERLRLLPVGRLDRDSSGLLLLTNDNKWLHPLTHPSYGHQKRYIVTVDNGTPKYDVLNELAEGVLL 295
[Close]
BLAST of NO06G04150 vs. NCBI_GenBank
Match: WP_045673688.1 (rRNA pseudouridine synthase [Paenibacillus beijingensis] >AJY78015.1 RNA pseudouridine synthase [Paenibacillus beijingensis])

HSP 1 Score: 148.3 bits (373), Expect = 4.900e-32
Identity = 84/160 (52.50%), Postives = 109/160 (68.12%), Query Frame = 0 
Query:  205 RLNKMLAHAGVASRRSAEEMIAQGKVSVNGVIVLDVGCKVDPSVDSITVEGKAAPVALRKASEVVWMKVNKPKGTLTSITDSKGRKCLGDLVESAVEKRLLPVGRLDRDTAGLLLMTNDNEAINLLTHPSFGHVKLYKAVVEKGMPSVHVLHQLKRGLVL 365
            RL K+LA AGVASRR  EE+I  G+V VNG  V ++G K DPS D ITV GK  P+   ++ + V++ +NKPKG +TS TD +GRK + D +E  +++R+ P+GRLD DT GLLL+TND E  NLLTHPS    K Y A V KG+P    L QLK+G+ L
Sbjct:    3 RLQKILAQAGVASRRKCEELIQSGQVEVNGQTVTELGTKADPSADIITVNGK--PI---RSEKKVYLMMNKPKGVITSATDPQGRKVVTDYLE-GIKERVYPIGRLDYDTEGLLLLTNDGEFANLLTHPSHHVPKTYLATV-KGVPHGSALDQLKKGIKL 155
[Close]
BLAST of NO06G04150 vs. NCBI_GenBank
Match: CBJ30337.1 (pseudouridine synthase [Ectocarpus siliculosus])

HSP 1 Score: 147.9 bits (372), Expect = 6.400e-32
Identity = 75/169 (44.38%), Postives = 108/169 (63.91%), Query Frame = 0 
Query:  201 GATTRLNKMLAHAGVASRRSAEEMIAQGKVSVNGVIVLDVGCKVDPSVDSITVEGKAAPVALRKASEVVWMKVNKPKGTLTSITDSKGRKCLGDLVESAVEKRLLPVGRLDRDTAGLLLMTNDNEAINLLTHPSFGHVKLYKAVVEKGMPSVHVLHQLKRGLVLLEEEN 370
            G   RL K L+HAG+ SRR  E+MI  G+V VNG +V ++G KV+P  D + V+G    V++R  SE+ W+ +NKPKG + +++D  GR      +   +E RL+PVGRLDRD+ GLLL+TN+N  IN LTHPS+GH K Y+  +  G P+      L +G++L  + N
Sbjct:   32 GGEERLQKFLSHAGLDSRRQCEQMILDGRVRVNGKMVKELGVKVNPRRDQVHVDGNR--VSMRPDSEIKWVALNKPKGVICTVSDELGRPTAVASIPRGLEMRLVPVGRLDRDSCGLLLLTNENSWINPLTHPSYGHEKTYRVALTSGYPTTEEWDVLTQGMLLDGDPN 198
[Close]
BLAST of NO06G04150 vs. NCBI_GenBank
Match: WP_028599813.1 (rRNA pseudouridine synthase [Paenibacillus pasadenensis] >PLT45606.1 Ribosomal large subunit pseudouridine synthase B [Paenibacillus pasadenensis])

HSP 1 Score: 147.5 bits (371), Expect = 8.300e-32
Identity = 83/160 (51.88%), Postives = 109/160 (68.12%), Query Frame = 0 
Query:  205 RLNKMLAHAGVASRRSAEEMIAQGKVSVNGVIVLDVGCKVDPSVDSITVEGKAAPVALRKASEVVWMKVNKPKGTLTSITDSKGRKCLGDLVESAVEKRLLPVGRLDRDTAGLLLMTNDNEAINLLTHPSFGHVKLYKAVVEKGMPSVHVLHQLKRGLVL 365
            RL K+LA AGVASRR  EE+IA G V VNG +V ++G K DP+ D ITV+GK  P+A  K    +++ +NKPKG +TS +D KGRK + D +   +++R+ PVGRLD DT GLLL+TND E  NLLTHPS    K Y A V KG+P    L +L++G+ L
Sbjct:    3 RLQKILAAAGVASRRKCEELIASGAVEVNGKVVRELGTKADPATDEITVKGK--PIASEKK---IYLMMNKPKGVITSASDPKGRKIVSDFL-PGIKERVYPVGRLDYDTEGLLLLTNDGEFANLLTHPSHHVPKTYLATV-KGVPHGSALERLQKGIKL 155
[Close]
BLAST of NO06G04150 vs. NCBI_GenBank
Match: WP_013310759.1 (MULTISPECIES: rRNA pseudouridine synthase [Paenibacillus] >ADM70611.1 RNA pseudouridine synthase [Paenibacillus polymyxa E681] >ALA42775.1 RNA pseudouridine synthase [Paenibacillus peoriae] >ODA07084.1 pseudouridine synthase [Paenibacillus polymyxa] >SFR06617.1 23S rRNA pseudouridine2605 synthase [Paenibacillus sp. cl130] >APQ60045.1 RNA pseudouridine synthase [Paenibacillus polymyxa] >OME72751.1 pseudouridine synthase [Paenibacillus peoriae])

HSP 1 Score: 146.0 bits (367), Expect = 2.400e-31
Identity = 83/160 (51.88%), Postives = 109/160 (68.12%), Query Frame = 0 
Query:  205 RLNKMLAHAGVASRRSAEEMIAQGKVSVNGVIVLDVGCKVDPSVDSITVEGKAAPVALRKASEVVWMKVNKPKGTLTSITDSKGRKCLGDLVESAVEKRLLPVGRLDRDTAGLLLMTNDNEAINLLTHPSFGHVKLYKAVVEKGMPSVHVLHQLKRGLVL 365
            RL K+LA AGVASRR  EE+I  G V VNGVIV ++G K DP  D ITV+G+  P+   K  + +++ +NKPKG +TS +D +GRK + D ++  V++R+ PVGRLD DT GLLL+TND E  NLLTHP     K Y A V KG+P    L +LK+G+VL
Sbjct:    3 RLQKILAQAGVASRRKCEELIQAGSVEVNGVIVTELGTKADPDQDMITVKGR--PI---KTEKKIYLMMNKPKGVITSASDPEGRKIVSDYLK-GVKERVYPVGRLDYDTEGLLLLTNDGEFANLLTHPKHHVPKTYVATV-KGVPHGTELDKLKKGIVL 155
[Close]
BLAST of NO06G04150 vs. NCBI_GenBank
Match: WP_035297859.1 (rRNA pseudouridine synthase [Brevibacillus thermoruber])

HSP 1 Score: 146.0 bits (367), Expect = 2.400e-31
Identity = 80/160 (50.00%), Postives = 115/160 (71.88%), Query Frame = 0 
Query:  205 RLNKMLAHAGVASRRSAEEMIAQGKVSVNGVIVLDVGCKVDPSVDSITVEGKAAPVALRKASEVVWMKVNKPKGTLTSITDSKGRKCLGDLVESAVEKRLLPVGRLDRDTAGLLLMTNDNEAINLLTHPSFGHVKLYKAVVEKGMPSVHVLHQLKRGLVL 365
            RL K+LAHAGVASRR  EE+I QG+V VNG +V ++G +VDP  D I V+G+  PV L    + V++ + KP G +TS++D +GR+ + DL++  V++R+ PVGRLD DT+GLLLMTND E  N+L HPS+   K+Y+A V +G+PS   +++L RG+ L
Sbjct:    3 RLQKVLAHAGVASRRHCEELIVQGRVQVNGKVVRELGTRVDPGTDRIAVDGR--PVRL---EQHVYLLLYKPTGVITSVSDPQGRRVVTDLLK-GVKERVYPVGRLDYDTSGLLLMTNDGELANMLAHPSYEIDKVYRAWV-RGVPSPEKVNRLARGIKL 155
[Close]
BLAST of NO06G04150 vs. NCBI_GenBank
Match: WP_071641426.1 (MULTISPECIES: rRNA pseudouridine synthase [Paenibacillus] >APB70772.1 rRNA pseudouridine synthase [Paenibacillus polymyxa] >OMF49192.1 pseudouridine synthase [Paenibacillus peoriae] >PPQ47641.1 rRNA pseudouridine synthase [Paenibacillus peoriae])

HSP 1 Score: 146.0 bits (367), Expect = 2.400e-31
Identity = 83/160 (51.88%), Postives = 109/160 (68.12%), Query Frame = 0 
Query:  205 RLNKMLAHAGVASRRSAEEMIAQGKVSVNGVIVLDVGCKVDPSVDSITVEGKAAPVALRKASEVVWMKVNKPKGTLTSITDSKGRKCLGDLVESAVEKRLLPVGRLDRDTAGLLLMTNDNEAINLLTHPSFGHVKLYKAVVEKGMPSVHVLHQLKRGLVL 365
            RL K+LA AGVASRR  EE+I  G V VNGVIV ++G K DP  D ITV+G+  P+   K  + +++ +NKPKG +TS +D +GRK + D ++  V++R+ PVGRLD DT GLLL+TND E  NLLTHP     K Y A V KG+P    L +LK+G+VL
Sbjct:    3 RLQKILAQAGVASRRKCEELIQAGSVEVNGVIVTELGTKADPDQDMITVKGR--PI---KTEKKIYLMMNKPKGVITSASDPEGRKIVSDYLK-GVKERVYPVGRLDYDTEGLLLLTNDGEFANLLTHPKHHVPKTYVATV-KGVPHGTELDKLKKGIVL 155
[Close]
BLAST of NO06G04150 vs. NCBI_GenBank
Match: WP_023554481.1 (rRNA pseudouridine synthase [Brevibacillus panacihumi] >EST56103.1 RNA pseudouridine synthase [Brevibacillus panacihumi W25])

HSP 1 Score: 145.6 bits (366), Expect = 3.200e-31
Identity = 78/160 (48.75%), Postives = 114/160 (71.25%), Query Frame = 0 
Query:  205 RLNKMLAHAGVASRRSAEEMIAQGKVSVNGVIVLDVGCKVDPSVDSITVEGKAAPVALRKASEVVWMKVNKPKGTLTSITDSKGRKCLGDLVESAVEKRLLPVGRLDRDTAGLLLMTNDNEAINLLTHPSFGHVKLYKAVVEKGMPSVHVLHQLKRGLVL 365
            RL K++AHAG+ASRR  EE+I QGKV VNG +V ++G KVDP+ D I VEG+  P+   +  + V++ +NKP G +TS++D +GR+ + DL+   +++R+ PVGRLD +T+GLLL+TND E  N L HPS+   K+Y+A V KG+P+   L  L RG++L
Sbjct:    3 RLQKVMAHAGIASRRHCEELIMQGKVQVNGQVVRELGTKVDPTKDKILVEGR--PI---RTEQHVYLMLNKPTGVITSVSDPRGRRVVTDLLR-GLKERVYPVGRLDYNTSGLLLLTNDGELANKLAHPSYEIDKVYRAWV-KGVPTADKLGMLARGIML 155
[Close]
BLAST of NO06G04150 vs. NCBI_GenBank
Match: WP_023989197.1 (MULTISPECIES: rRNA pseudouridine synthase [Paenibacillus] >KEO79333.1 RNA pseudouridine synthase [Paenibacillus polymyxa] >AIW40499.1 RNA pseudouridine synthase [Paenibacillus polymyxa CR1] >KOS01305.1 pseudouridine synthase [Paenibacillus polymyxa] >KYG95862.1 pseudouridine synthase [Paenibacillus polymyxa] >ODB51296.1 pseudouridine synthase [Paenibacillus polymyxa] >ODB62312.1 pseudouridine synthase [Paenibacillus polymyxa] >AOK88732.1 pseudouridine synthase [Paenibacillus polymyxa] >APB75427.1 rRNA pseudouridine synthase [Paenibacillus polymyxa] >OMF31617.1 pseudouridine synthase [Paenibacillus peoriae] >OMF71759.1 pseudouridine synthase [Paenibacillus peoriae] >OMF79660.1 pseudouridine synthase [Paenibacillus peoriae] >PNQ80422.1 rRNA pseudouridine synthase [Paenibacillus sp. F4] >POR27665.1 rRNA pseudouridine synthase [Paenibacillus polymyxa])

HSP 1 Score: 145.6 bits (366), Expect = 3.200e-31
Identity = 82/160 (51.25%), Postives = 109/160 (68.12%), Query Frame = 0 
Query:  205 RLNKMLAHAGVASRRSAEEMIAQGKVSVNGVIVLDVGCKVDPSVDSITVEGKAAPVALRKASEVVWMKVNKPKGTLTSITDSKGRKCLGDLVESAVEKRLLPVGRLDRDTAGLLLMTNDNEAINLLTHPSFGHVKLYKAVVEKGMPSVHVLHQLKRGLVL 365
            RL K+LA AGVASRR  EE+I  G V VNGV+V ++G K DP  D ITV+G+  P+   K  + +++ +NKPKG +TS +D +GRK + D ++  V++R+ PVGRLD DT GLLL+TND E  NLLTHP     K Y A V KG+P    L +LK+G+VL
Sbjct:    3 RLQKILAQAGVASRRKCEELIQAGSVEVNGVVVTELGTKADPDQDMITVKGR--PI---KTEKKIYLMMNKPKGVITSASDPEGRKIVSDYLK-GVKERVYPVGRLDYDTEGLLLLTNDGEFANLLTHPKHHVPKTYVATV-KGVPHGTELDKLKKGIVL 155
[Close]
BLAST of NO06G04150 vs. NCBI_GenBank
Match: WP_025721016.1 (rRNA pseudouridine synthase [Paenibacillus sp. 1-18])

HSP 1 Score: 145.6 bits (366), Expect = 3.200e-31
Identity = 83/160 (51.88%), Postives = 109/160 (68.12%), Query Frame = 0 
Query:  205 RLNKMLAHAGVASRRSAEEMIAQGKVSVNGVIVLDVGCKVDPSVDSITVEGKAAPVALRKASEVVWMKVNKPKGTLTSITDSKGRKCLGDLVESAVEKRLLPVGRLDRDTAGLLLMTNDNEAINLLTHPSFGHVKLYKAVVEKGMPSVHVLHQLKRGLVL 365
            RL K+LA AGVASRR  EE+I  G V VNGVIV ++G K DP  D ITV+G+  P+   K  + +++ +NKPKG +TS +D +GRK + D ++  V++R+ PVGRLD DT GLLL+TND E  NLLTHP     K Y A V KG+P    L +LK+G+VL
Sbjct:    3 RLQKILAQAGVASRRKCEELIQAGSVEVNGVIVTELGTKADPDQDIITVKGR--PI---KTEKKIYLMMNKPKGVITSASDPEGRKIVSDYLK-GVKERVYPVGRLDYDTEGLLLLTNDGEFANLLTHPKHHVPKTYVATV-KGVPHGTELDKLKKGIVL 155
The following BLAST results are available for this feature:
BLAST of NO06G04150 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes)
Total hits: 20
Match NameE-valueIdentityDescription
CEM01544.17.700e-3847.45unnamed protein product [Vitrella brassicaformis C... [more]
WP_045673688.14.900e-3252.50rRNA pseudouridine synthase [Paenibacillus beijing... [more]
CBJ30337.16.400e-3244.38pseudouridine synthase [Ectocarpus siliculosus][more]
WP_028599813.18.300e-3251.88rRNA pseudouridine synthase [Paenibacillus pasaden... [more]
WP_013310759.12.400e-3151.88MULTISPECIES: rRNA pseudouridine synthase [Paeniba... [more]
WP_035297859.12.400e-3150.00rRNA pseudouridine synthase [Brevibacillus thermor... [more]
WP_071641426.12.400e-3151.88MULTISPECIES: rRNA pseudouridine synthase [Paeniba... [more]
WP_023554481.13.200e-3148.75rRNA pseudouridine synthase [Brevibacillus panacih... [more]
WP_023989197.13.200e-3151.25MULTISPECIES: rRNA pseudouridine synthase [Paeniba... [more]
WP_025721016.13.200e-3151.88rRNA pseudouridine synthase [Paenibacillus sp. 1-1... [more]

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Relationships

This gene is member of the following syntenic_region feature(s):

Feature NameUnique NameSpeciesType
nonsL091nonsL091Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ngnoR147ngnoR147Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region


The following polypeptide feature(s) derives from this gene:

Feature NameUnique NameSpeciesType
NO06G04150.1NO06G04150.1-proteinNannochloropsis oceanica (N. oceanica IMET1)polypeptide


The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
NO06G04150.1NO06G04150.1Nannochloropsis oceanica (N. oceanica IMET1)mRNA


Sequences
The following sequences are available for this feature:

gene sequence

>NO06G04150 ID=NO06G04150|Name=NO06G04150|organism=Nannochloropsis oceanica|type=gene|length=1192bp
AACCAACAAATACACACCATGCCTCCCCTTATGCAAGCACAGGTGTCATC
ATGGTGCCAAGAAGAAGGTCTCGAGCAATAGGTGCACCAGTACGACGTAT
GGTTGGGGTCCTGACACTCTTATTTGCCGGCCACAGCTTATTCGTCACCA
CCCATGCCCGTGCTGCATTTCTGCTGCCCTCCGCCGCCTCGGGTCGCTGC
CCTTCATCACGCTCCCACCCAGACCCTGCGACAGGAAGGAGGAGACACAA
TGTCCCACATATGGCACGTACCAGCGGTAGCAGCGGAGGTGGCGGTGGTG
GTGGCCGGAGGGATGGAGGAGGCAATTACAGAGGAGCCAACCGGGATGCG
CCACGCCGACGCTTTAATGACGACGACGACGAACGGCGCCGCCGCCGCAG
CAGCAGCAATAGCAAGCCCCCACATGGCAGCAATAGAGACAGTTACAGTG
GACGCAGTACATCAGCTACAGCAGCCGACACAAACGACATGCGTGGACAG
GGACTACGCAAGGCTTTGTCGGCACTAACGGGCCTCCGTGGTGACAAACC
CTCCGCTCGAAAGGGCCAAGCAGGCACATCCAGAAAGAAATCGTCTCCCT
CCTCCCCATCCATAGCACCGAAAGGTAGCGTCGTGTCCCTCTCTCCCCGT
GGGGCCACTACGCGGCTGAATAAGATGCTGGCGCATGCCGGAGTCGCCTC
TCGACGAAGCGCAGAGGAAATGATTGCTCAAGGGAAGGTCAGCGTGAATG
GCGTGATTGTGTTGGACGTGGGTTGCAAGGTTGATCCATCTGTGGACTCC
ATAACGGTGGAGGGGAAGGCTGCGCCGGTAGCGTTGCGGAAGGCGAGTGA
GGTGGTGTGGATGAAGGTGAACAAGCCGAAGGGCACTCTGACGTCAATCA
CGGACAGCAAAGGCCGTAAATGTCTGGGGGATCTGGTGGAAAGCGCGGTG
GAAAAGCGGCTTTTGCCTGTTGGACGGTTGGACAGGGACACTGCGGGATT
GTTGTTGATGACGAATGACAACGAGGCAATCAATCTGTTGACGCACCCGT
CCTTTGGCCACGTTAAGTTGTACAAGGCTGTGGTGGAGAAGGGCATGCCG
TCTGTGCATGTTTTGCACCAGCTTAAGAGGGGCTTGGTGCTCTTGGAGGA
GGAGAACGGGGGTAAAGAGGAGCTGGAGGGGGGGTAAGGGGA
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protein sequence of NO06G04150.1

>NO06G04150.1-protein ID=NO06G04150.1-protein|Name=NO06G04150.1|organism=Nannochloropsis oceanica|type=polypeptide|length=379bp
MVPRRRSRAIGAPVRRMVGVLTLLFAGHSLFVTTHARAAFLLPSAASGRC
PSSRSHPDPATGRRRHNVPHMARTSGSSGGGGGGGRRDGGGNYRGANRDA
PRRRFNDDDDERRRRRSSSNSKPPHGSNRDSYSGRSTSATAADTNDMRGQ
GLRKALSALTGLRGDKPSARKGQAGTSRKKSSPSSPSIAPKGSVVSLSPR
GATTRLNKMLAHAGVASRRSAEEMIAQGKVSVNGVIVLDVGCKVDPSVDS
ITVEGKAAPVALRKASEVVWMKVNKPKGTLTSITDSKGRKCLGDLVESAV
EKRLLPVGRLDRDTAGLLLMTNDNEAINLLTHPSFGHVKLYKAVVEKGMP
SVHVLHQLKRGLVLLEEENGGKEELEGG*
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Synonyms
Publications