NO06G01960, NO06G01960 (gene) Nannochloropsis oceanica

Overview
NameNO06G01960
Unique NameNO06G01960
Typegene
OrganismNannochloropsis oceanica (N. oceanica IMET1)
Sequence length1879
Alignment locationchr6:583829..585707 -

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Properties
Property NameValue
DescriptionRibonuclease h
Mutants
Expression

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
chr6genomechr6:583829..585707 -
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
PRJNA7699582024-08-13
GSE1786722023-12-15
PRJNA9336932023-10-26
PRJNA9434492023-07-19
GSE1499042020-10-10
NoIMET1_WT_HS2020-09-29
GSE1396152020-03-11
PRJNA2413822020-03-11
BLASTP analysis of N. oceanica IMET1 genes2019-07-11
InterPro analysis for N. oceanica IMET1 genes2019-07-11
GO annotation for IMET1v2 genes2019-07-10
PRJNA1821802019-04-25
Gene prediction for N. oceanica IMET12017-10-24
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004523RNA-DNA hybrid ribonuclease activity
GO:0003676nucleic acid binding
GO:0004523RNA-DNA hybrid ribonuclease activity
GO:0003676nucleic acid binding
Vocabulary: INTERPRO
TermDefinition
IPR012337RNaseH-like_dom
IPR009027Ribosomal_L9/RNase_H1_N
IPR002156RNaseH_domain
IPR011320RNase_H1_N
Vocabulary: Biological Process
TermDefinition
GO:0090502RNA phosphodiester bond hydrolysis, endonucleolytic
Homology
BLAST of NO06G01960 vs. NCBI_GenBank
Match: EWM26257.1 (ribonuclease h [Nannochloropsis gaditana])

HSP 1 Score: 168.3 bits (425), Expect = 5.100e-38
Identity = 124/432 (28.70%), Postives = 187/432 (43.29%), Query Frame = 0
Query:    2 KFYAVRLPIRAIFTSWPECNAVVSRANFANFKSFPTLEEARVYMEDGLALQEQPDEDGGEQQHVEKFMGDGGKDYEQRAGKPWGLKQESEGSRDGIGEGXXXXXXXXXXXXXXXXXFMNTAESKREEQVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVISLLSDSGDEDYSEDLRGGQKRRLKSDWGQ---------AEEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKAHVITSGKLFFDGGSRGNPGIAGCGAVMYDELSGRKLWSLSAFLGHDRTNNQAEYAALCFGLEEALKRGVSSVQVFGDSTLVVKQVQGAWKVKNAGMRVWHSKALALGKKFKRFRICYIPREENKVADALANEGMDGRKEGGAVVVEE 425
            K+YAV++P+RAIFTSW EC+AVV+ A  A FKSF +L+EAR ++EDGL  Q+   +    + H  +   +  +D +       G+ +E E + +G+                    + N+    R+E +                               + + +  +G    + +    +++R K +  Q         A+E                                                                                +   + +G LFFDGG+RGNPG++G GAVM+D    ++LW+LS FLG D+TNNQAEY AL  GLEEA +RGV ++   GDS LV++Q++G WKVKN  +   H++  AL ++F+     ++PR EN VADALAN  MD  K     VVEE
Sbjct:   48 KYYAVKVPMRAIFTSWSECHAVVNGAGNATFKSFSSLDEARAFVEDGLESQQYTGKKSSRESHGLQIFSE--QDLKDCGMLQEGIDEEREQNIEGL--------------------YANSGAETRKEYM----------------------ERSSTPHTDMDNHVCGTGGARCAANEERRKRKRTKEEHAQDVSQEQDAFAQECGDAPLTGRLGSRIGGGSICTVGMNELAGEGRGDQENEDDDFDDESSRVTEPIPAIILSPNDTSPNIPSEDVSATALLQRRPRFVDAGVLFFDGGARGNPGVSGGGAVMFD-CDNKQLWTLSVFLGSDKTNNQAEYLALYHGLEEAARRGVRTLHARGDSLLVIRQMEGVWKVKNKAIMRLHAQVKALARRFRALTFQHVPRAENTVADALANAAMD--KRHSIPVVEE 432          
BLAST of NO06G01960 vs. NCBI_GenBank
Match: PSC67966.1 (bifunctional RNase H acid phosphatase [Micractinium conductrix])

HSP 1 Score: 131.3 bits (329), Expect = 6.900e-27
Identity = 66/134 (49.25%), Postives = 91/134 (67.91%), Query Frame = 0
Query:  284 KLFFDGGSRGNPGIAGCGAVMYDELSGRKLWSLSAFLGHDRTNNQAEYAALCFGLEEALKRGVSSVQVFGDSTLVVKQVQGAWKVKNAGMRVWHSKALALGKKFKRFRICYIPREENKVADALANEGMDGRKEG 418
            +L FDG S+ NPG AG GAV+Y+E  G ++  L  ++G   TNNQAEYA L  GL  AL+ G   V+V GDSTL+V+QV G W+VKN G+R +H+ A+AL ++F  F    +PR +N VADAL+N+ +D  + G
Sbjct:  166 RLEFDGASKQNPGPAGFGAVIYEEAEGEEVHRLCQYMGDLCTNNQAEYAGLIAGLAAALELGCRQVKVQGDSTLIVQQVLGNWQVKNEGLRPYHAAAVALARRFAGFEAKQVPRAQNAVADALSNQAIDEFRSG 299          
BLAST of NO06G01960 vs. NCBI_GenBank
Match: PRW20551.1 (ribonuclease H [Chlorella sorokiniana])

HSP 1 Score: 128.3 bits (321), Expect = 5.800e-26
Identity = 65/134 (48.51%), Postives = 88/134 (65.67%), Query Frame = 0
Query:  284 KLFFDGGSRGNPGIAGCGAVMYDELSGRKLWSLSAFLGHDRTNNQAEYAALCFGLEEALKRGVSSVQVFGDSTLVVKQVQGAWKVKNAGMRVWHSKALALGKKFKRFRICYIPREENKVADALANEGMDGRKEG 418
            +L FDGGS+GNPG AG GAV+YD+ SG ++  L  ++G   TNNQAEYA L  GL  AL+ G S ++V GDS LV+ QV G W+VK  G+R +H  A  L ++F  F    + RE N+ ADAL+N+ +D  + G
Sbjct:  139 RLEFDGGSKGNPGPAGFGAVLYDDASGVEVARLCQYIGDHNTNNQAEYAGLIAGLAAALELGCSRLKVQGDSMLVINQVLGRWQVKTDGLRPYHRLAQQLARRFDHFEAKQVLREFNQAADALSNQAVDDYRSG 272          
BLAST of NO06G01960 vs. NCBI_GenBank
Match: XP_022841041.1 (Ribonuclease H1, N-terminal [Ostreococcus tauri] >CEG01575.1 Ribonuclease H1, N-terminal [Ostreococcus tauri])

HSP 1 Score: 126.3 bits (316), Expect = 2.200e-25
Identity = 62/128 (48.44%), Postives = 90/128 (70.31%), Query Frame = 0
Query:  285 LFFDGGSRGNPGIAGCGAVMYDELSGRKLWSLSAFLGHDRTNNQAEYAALCFGLEEALKRGVSSVQVFGDSTLVVKQVQGAWKVKNAGMRVWHSKALALGKKFKRFRICYIPREENKVADALANEGMD 413
            L FDG SRGNPG AG GA++  +   R +  L  +LG +RT N+AEYAALC GL +A++ G++ ++V GDS L+V QV G++K+K+  +R  H++A++L KKF  F+I ++ RE NK AD LAN  +D
Sbjct:  149 LEFDGASRGNPGEAGAGALLRRKRDDRVVEELLEYLGSERTVNEAEYAALCLGLRKAIELGITKIEVRGDSKLIVNQVDGSFKLKSENLRSMHAEAVSLKKKFAEFKISHVKREFNKHADHLANMAVD 276          
BLAST of NO06G01960 vs. NCBI_GenBank
Match: XP_001419204.1 (predicted protein, partial [Ostreococcus lucimarinus CCE9901] >ABO97497.1 predicted protein, partial [Ostreococcus lucimarinus CCE9901])

HSP 1 Score: 124.4 bits (311), Expect = 8.400e-25
Identity = 60/126 (47.62%), Postives = 90/126 (71.43%), Query Frame = 0
Query:  287 FDGGSRGNPGIAGCGAVMYDELSGRKLWSLSAFLGHDRTNNQAEYAALCFGLEEALKRGVSSVQVFGDSTLVVKQVQGAWKVKNAGMRVWHSKALALGKKFKRFRICYIPREENKVADALANEGMD 413
            FDG SRGNPG AG GA++  +   R +  L  +LG++RT N+AEYAALC GL +A++ G++ ++V GDS L+V QV+G++K+K+A ++  H++A  L +KF  F+I ++ RE NK AD LAN  +D
Sbjct:    1 FDGASRGNPGEAGAGALLRRKRDDRIVEELLEYLGNERTVNEAEYAALCLGLRKAVELGITKIEVRGDSKLIVNQVEGSFKLKSANLKSMHAEACELKEKFTEFKISHVKREFNKHADHLANMAVD 126          
BLAST of NO06G01960 vs. NCBI_GenBank
Match: WP_013275740.1 (ribonuclease H [Thermosediminibacter oceani] >ADL07698.1 ribonuclease H [Thermosediminibacter oceani DSM 16646])

HSP 1 Score: 119.8 bits (299), Expect = 2.100e-23
Identity = 63/128 (49.22%), Postives = 89/128 (69.53%), Query Frame = 0
Query:  285 LFFDGGSRGNPGIAGCGAVMYDELSGRKLWSLSAFLGHDRTNNQAEYAALCFGLEEALKRGVSSVQVFGDSTLVVKQVQGAWKVKNAGMRVWHSKALALGKKFKRFRICYIPREENKVADALANEGMD 413
            ++ DG SRGNPG AG G V+ DE +G  L  +S ++G   TNN AEY AL   L+EAL+ G   + +F DS L+VKQ+ G +++KN G++  +++A+ L K+FKRF I +I RE+NK AD LANEG+D
Sbjct:    6 VYTDGASRGNPGDAGIGIVILDE-NGNTLKEISDYIG-QTTNNIAEYTALVTALKEALEMGCEEIGIFSDSELMVKQINGEYQIKNEGIKRLYAQAMELLKEFKRFSISHIRREQNKRADELANEGID 131          
BLAST of NO06G01960 vs. NCBI_GenBank
Match: WP_068748886.1 (hypothetical protein [Thermovenabulum gondwanense] >KYO65184.1 14.7 kDa ribonuclease H-like protein [Thermovenabulum gondwanense])

HSP 1 Score: 119.0 bits (297), Expect = 3.500e-23
Identity = 65/128 (50.78%), Postives = 86/128 (67.19%), Query Frame = 0
Query:  285 LFFDGGSRGNPGIAGCGAVMYDELSGRKLWSLSAFLGHDRTNNQAEYAALCFGLEEALKRGVSSVQVFGDSTLVVKQVQGAWKVKNAGMRVWHSKALALGKKFKRFRICYIPREENKVADALANEGMD 413
            L+ DG SRGNPG AG G V+ DE  G  +  +S ++G   TNN AEY AL  GL+EAL+     V VF DS L+VKQ+QG ++VKN G++  + +   L K+FK+F IC++ RE NK AD LANEG+D
Sbjct:    6 LYTDGASRGNPGDAGIGIVIIDE-EGNIVKEISDYIG-QTTNNIAEYKALITGLKEALELECEEVNVFTDSELMVKQIQGEYQVKNEGLKPLYKEVFELIKEFKKFSICHVRREMNKRADELANEGID 131          
BLAST of NO06G01960 vs. NCBI_GenBank
Match: OGC46515.1 (hypothetical protein A2V49_00500 [candidate division WWE3 bacterium RBG_19FT_COMBO_34_6])

HSP 1 Score: 119.0 bits (297), Expect = 3.500e-23
Identity = 60/128 (46.88%), Postives = 87/128 (67.97%), Query Frame = 0
Query:  285 LFFDGGSRGNPGIAGCGAVMYDELSGRKLWSLSAFLGHDRTNNQAEYAALCFGLEEALKRGVSSVQVFGDSTLVVKQVQGAWKVKNAGMRVWHSKALALGKKFKRFRICYIPREENKVADALANEGMD 413
            L+ DGG+RGNPG    G V+YDE +  K++ LS ++G   TNN+AEY +L  G+  AL +G+ ++ VF DS L++KQV G++KVKN  ++V+++K + L KKF      +I REENK AD L NE +D
Sbjct:    5 LYTDGGARGNPGPGAIGVVIYDE-NKNKIYELSKYIGMS-TNNEAEYTSLIEGIGSALDKGIKNLNVFMDSELIIKQVNGSYKVKNERLKVFYNKLVLLRKKFDFINFVHIKREENKQADLLVNEALD 130          
BLAST of NO06G01960 vs. NCBI_GenBank
Match: WP_066351093.1 (hypothetical protein [Fervidicola ferrireducens] >KXG78496.1 14.7 kDa ribonuclease H-like protein [Fervidicola ferrireducens])

HSP 1 Score: 118.6 bits (296), Expect = 4.600e-23
Identity = 62/129 (48.06%), Postives = 88/129 (68.22%), Query Frame = 0
Query:  284 KLFFDGGSRGNPGIAGCGAVMYDELSGRKLWSLSAFLGHDRTNNQAEYAALCFGLEEALKRGVSSVQVFGDSTLVVKQVQGAWKVKNAGMRVWHSKALALGKKFKRFRICYIPREENKVADALANEGMD 413
            K++ DG SRGNPG AG G VM DE +G  +  +S ++G   TNN AEY AL   L+EAL+ G   ++VF DS L+VKQ+ G +++KN G++  +S+ + L K+F+ F I ++ RE NK AD LANEG+D
Sbjct:    5 KVYVDGASRGNPGDAGIGIVMLDE-NGNVVKEMSDYIG-QTTNNIAEYTALIIALKEALEMGCEEIEVFSDSELMVKQINGQYQIKNEGIKRLYSQVVQLMKEFRSFSINHVRREYNKRADELANEGID 131          
BLAST of NO06G01960 vs. NCBI_GenBank
Match: WP_006584292.1 (ribonuclease H [Thermanaerovibrio velox] >EHM10798.1 ribonuclease HI [Thermanaerovibrio velox DSM 12556])

HSP 1 Score: 118.2 bits (295), Expect = 6.000e-23
Identity = 68/141 (48.23%), Postives = 89/141 (63.12%), Query Frame = 0
Query:  284 KLFFDGGSRGNPGIAGCGAVMYDELSGRKLWSLSAFLGHDRTNNQAEYAALCFGLEEALKRGVSSVQVFGDSTLVVKQVQGAWKVKNAGMRVWHSKALALGKKFKRFRICYIPREENKVADALANEGMDGRKEGGAVVVEE 425
            K +FDG SRGNPG+AG GA++ D+  G  +W    FLGH RTNN+AEY AL   LEE  +RG+ SV V GDS LV+ QV G WK+    +R   S+   L +  +     +IPREEN+ AD L+NE +D R++  AV  EE
Sbjct:    3 KGYFDGASRGNPGVAGAGALLVDD-GGSVIWEYRGFLGH-RTNNEAEYQALIALLEEVRRRGIRSVLVMGDSQLVISQVTGKWKINMPHLRALASRVRELCRGLE-VSFKWIPREENRQADRLSNEAID-RRDLRAVGFEE 139          
The following BLAST results are available for this feature:
BLAST of NO06G01960 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes)
Total hits: 20
Match NameE-valueIdentityDescription
EWM26257.15.100e-3828.70ribonuclease h [Nannochloropsis gaditana][more]
PSC67966.16.900e-2749.25bifunctional RNase H acid phosphatase [Micractiniu... [more]
PRW20551.15.800e-2648.51ribonuclease H [Chlorella sorokiniana][more]
XP_022841041.12.200e-2548.44Ribonuclease H1, N-terminal [Ostreococcus tauri] >... [more]
XP_001419204.18.400e-2547.62predicted protein, partial [Ostreococcus lucimarin... [more]
WP_013275740.12.100e-2349.22ribonuclease H [Thermosediminibacter oceani] >ADL0... [more]
WP_068748886.13.500e-2350.78hypothetical protein [Thermovenabulum gondwanense]... [more]
OGC46515.13.500e-2346.88hypothetical protein A2V49_00500 [candidate divisi... [more]
WP_066351093.14.600e-2348.06hypothetical protein [Fervidicola ferrireducens] >... [more]
WP_006584292.16.000e-2348.23ribonuclease H [Thermanaerovibrio velox] >EHM10798... [more]

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Relationships

This gene is member of the following syntenic_region feature(s):

Feature NameUnique NameSpeciesType
nonsL091nonsL091Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ncniR028ncniR028Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ngnoR147ngnoR147Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region


This gene is orthologous to the following gene feature(s):

Feature NameUnique NameSpeciesType
NSK000263NSK000263Nannochloropsis salina (N. salina CCMP1776)gene


The following polypeptide feature(s) derives from this gene:

Feature NameUnique NameSpeciesType
NO06G01960.1NO06G01960.1-proteinNannochloropsis oceanica (N. oceanica IMET1)polypeptide


The following gene feature(s) are orthologous to this gene:

Feature NameUnique NameSpeciesType
jgi.p|Nanoce1779_2|567561gene_6505Nannochloropsis oceanica (N. oceanica CCMP1779)gene
Naga_100025g22gene4309Nannochloropsis gaditana (N. gaditana B-31)gene


The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
NO06G01960.1NO06G01960.1Nannochloropsis oceanica (N. oceanica IMET1)mRNA


Sequences
The following sequences are available for this feature:

gene sequence

>NO06G01960 ID=NO06G01960|Name=NO06G01960|organism=Nannochloropsis oceanica|type=gene|length=1879bp
ACAGGCAATGCCTCGAGGACGGCACCCACAGTCGTTGCGCCGAGCCTAAA
TCCGCCCTCCTTCCCCTCAGTCGAATCAAGTGCGCCTAGCACACCGCGGG
ACCGCGGGCAGCACCACTGCCACCACCACACTACAAGCGCAAGCCCCCAA
TGAAGTTTTACGCTGTCCGCCTTCCTATTCGCGCCATCTTCACCTCGTGG
CCGGAATGCAACGCCGTCGTTTCGCGGGCGAACTTTGCCAACTTCAAATC
CTTTCCTACGTTGGAGGAAGCTCGGGTGTACATGGAAGACGGGCTCGCGC
TGCAGGAGCAACCAGATGAAGACGGTGGGGAGCAGCAGCATGTGGAGAAA
TTCATGGGGGATGGTGGAAAGGATTACGAACAAAGAGCGGGTAAGCCATG
GGGTTTGAAGCAGGAGAGTGAAGGTTCTAGGGATGGGATCGGGGAGGGAG
GAGGGAGAGGGGGAGGAGGAGGAGAAGAAAGGAGGATGGTCTCACCGCCA
TTTATGAATACGGCGGAGTCGAAGAGAGAGGAGCAAGTTGGTGAAGGAGG
GCAAGATGCGAGGAAGCGGAAAGCACCACTTCGTGTCGTGGGCGTCGAGA
AGCGGGAAAGAGGAGGAGGAAAAGGAGGGGAGGTAATCAGTTTGCTGAGC
GACAGTGGAGACGAGGACTACAGCGAAGATTTGCGGGGAGGGCAAAAGAG
GCGACTGAAGAGTGATTGGGGACAAGCCGAAGAACGTCAAGACCAAAATG
ATGATGGGGAGAGCGAGGAGGAGTATAATGATGACAATGAGCGCTTCGAA
GCCACCCAAGCCATCCCTGCTTCCCCCCTCGCTCCTGTCCCCAAAGCCCC
TACTCGTCCTCCTCCTCTCACTATAGCCGCAGCAACTGCCGCAACTACTG
GCGCTAGTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGGTCGTGGTGGT
GATATTGCTACTGTTGCTACTGCCAAAGCGCATGTCATCACCTCCGGCAA
GCTATTCTTCGATGGCGGCTCGCGTGGGAATCCGGGCATTGCTGGTTGCG
GTGCTGTCATGTATGACGAGCTATCAGGGAGGAAACTCTGGTCTTTATCC
GCTTTTCTGGGGCACGATAGAACCAACAATCAAGCCGAATACGCTGCTTT
GTGCTTTGGGCTGGAGGAGGCATTGAAGCGAGGCGTGAGCAGTGTCCAGG
TCTTTGGAGACTCTACATTGGTAGTGAAGCAGGTGCAGGGGGCATGGAAG
GTCAAAAATGCCGGCATGAGAGTGTGGCATAGCAAGGCTCTGGCCCTGGG
GAAGAAGTTTAAACGGTTTCGGATTTGTTATATACCGAGGGAGGAGAACA
AGGTGGCGGACGCGTTGGCCAATGAAGGGATGGATGGACGGAAGGAAGGA
GGGGCGGTGGTCGTTGAGGAGTGATAAGGCTTGTAAATATGTGATGAATG
ATTAGTATAAAAAATAAAATAAAATGTGACGGAAGGGACTTCAGGATAGG
CGTCGATGATCATCAAGTCCAAGGATAAGCGTCCCCTTTGCCTCTTCAAA
AAGACCTCTTGGCGCTGATCCAATTCGAAATTTCCTGGCTTCTCCAATGC
ACCAGCCAAAGGGATAGGCTCAGCCATTCGACGAGTGCCTCCTGAGGCGC
CCCTCTAATCCACCCTCCTAATCCACCCTCACAATCCACCCTCCTAATCC
ACCCTCCTAATCCACCCCTCTAATCCACCCTCCTAATCCACCCACCAAAT
CCACCCTTCTAATCCACCCACCCACGGCACCGACAAATGAACCCAGACCG
TGCTAGATTTTCCCTTTATGCCGAATACGACTCACAACGAGCTGAATATG
GAAAAGCACAGAGCCATGACACGTGTCGT
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protein sequence of NO06G01960.1

>NO06G01960.1-protein ID=NO06G01960.1-protein|Name=NO06G01960.1|organism=Nannochloropsis oceanica|type=polypeptide|length=425bp
MKFYAVRLPIRAIFTSWPECNAVVSRANFANFKSFPTLEEARVYMEDGLA
LQEQPDEDGGEQQHVEKFMGDGGKDYEQRAGKPWGLKQESEGSRDGIGEG
GGRGGGGGEERRMVSPPFMNTAESKREEQVGEGGQDARKRKAPLRVVGVE
KRERGGGKGGEVISLLSDSGDEDYSEDLRGGQKRRLKSDWGQAEERQDQN
DDGESEEEYNDDNERFEATQAIPASPLAPVPKAPTRPPPLTIAAATAATT
GASAAAAAAAAAAGRGGDIATVATAKAHVITSGKLFFDGGSRGNPGIAGC
GAVMYDELSGRKLWSLSAFLGHDRTNNQAEYAALCFGLEEALKRGVSSVQ
VFGDSTLVVKQVQGAWKVKNAGMRVWHSKALALGKKFKRFRICYIPREEN
KVADALANEGMDGRKEGGAVVVEE*
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Synonyms
Publications