NO05G05420, NO05G05420 (gene) Nannochloropsis oceanica

Overview
NameNO05G05420
Unique NameNO05G05420
Typegene
OrganismNannochloropsis oceanica (N. oceanica IMET1)
Sequence length1569
Alignment locationchr5:1455781..1457349 -

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Properties
Property NameValue
DescriptionThioesterase superfamily member 4
Mutants
Expression

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
chr5genomechr5:1455781..1457349 -
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
PRJNA7699582024-08-13
GSE1786722023-12-15
PRJNA9336932023-10-26
PRJNA9434492023-07-19
GSE1499042020-10-10
NoIMET1_WT_HS2020-09-29
GSE1396152020-03-11
PRJNA2413822020-03-11
BLASTP analysis of N. oceanica IMET1 genes2019-07-11
InterPro analysis for N. oceanica IMET1 genes2019-07-11
PRJNA1821802019-04-25
Gene prediction for N. oceanica IMET12017-10-24
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR029069HotDog_dom
IPR006683Thioestr_supf
Homology
BLAST of NO05G05420 vs. NCBI_GenBank
Match: EWM27855.1 (thioesterase superfamily member 4 [Nannochloropsis gaditana])

HSP 1 Score: 299.3 bits (765), Expect = 1.300e-77
Identity = 161/231 (69.70%), Postives = 183/231 (79.22%), Query Frame = 0
Query:   57 TLRTSFPVLFAQLAAATAAVVAAISLPSPSLCETAHAGTEERRGERKAMREDGGKGEATSSATCNPSLFEHHDRVDTKLHRAYPEFLKFHLIHETLRGKEKIDGYEVYKDRRDDSIVAYARLGKLLSGHPDIIHGGSIAALLDNTMGVAFFAAKRGNGFTANLTINYKRPITCGTEVKVLARVEKVEGRKVFLRAEIRDAKDEAILYTEAKSLFITSQSPLLKGPKKIDIS 288
            T  +SF +L   + A +    A I+        TA A  +E +  ++ +   GG    T+  T       +HDRVDTKLHRAYPEFL++HLIHETLRGKEKI+GYEVYKDRRDDSIVA+ARLGKLLSGHPDIIHGGSIAALLDNTMGVAFFAA +GNGFTANLTINYKRPI CGTE+KVLARVE++EGRKVFLRAEIRDAKDEA+LYTEA SLFITSQSPLL GPKK+DIS
Sbjct:   52 TQNSSFSILLKTVVAGSFVGAAFIA------GHTAGASCDEVKSPQE-VNNVGGGAPVTAPYTVT-FASNYHDRVDTKLHRAYPEFLQYHLIHETLRGKEKIEGYEVYKDRRDDSIVAFARLGKLLSGHPDIIHGGSIAALLDNTMGVAFFAANKGNGFTANLTINYKRPIICGTEIKVLARVERIEGRKVFLRAEIRDAKDEAVLYTEATSLFITSQSPLLTGPKKVDIS 274          
BLAST of NO05G05420 vs. NCBI_GenBank
Match: XP_005852768.1 (thioesterase superfamily member 4 [Nannochloropsis gaditana CCMP526] >EKU23063.1 thioesterase superfamily member 4 [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 297.7 bits (761), Expect = 3.800e-77
Identity = 161/231 (69.70%), Postives = 182/231 (78.79%), Query Frame = 0
Query:   57 TLRTSFPVLFAQLAAATAAVVAAISLPSPSLCETAHAGTEERRGERKAMREDGGKGEATSSATCNPSLFEHHDRVDTKLHRAYPEFLKFHLIHETLRGKEKIDGYEVYKDRRDDSIVAYARLGKLLSGHPDIIHGGSIAALLDNTMGVAFFAAKRGNGFTANLTINYKRPITCGTEVKVLARVEKVEGRKVFLRAEIRDAKDEAILYTEAKSLFITSQSPLLKGPKKIDIS 288
            T  +SF +L   + A +    A I+        TA A  +E +  ++ +   GG    T+  T       +HDRVDTKLHRAYPEFL++HLIHETLRGKEKI+GYEVYKDRRDDSIVA+ARLGKLLSGHPDIIHGGSIAALLDNTMGVAFFAA +GNGFTANLTINYKRPI CGTE+KVLARVE+ EGRKVFLRAEIRDAKDEA+LYTEA SLFITSQSPLL GPKK+DIS
Sbjct:   52 TQNSSFSILLKTVVAGSFVGAAFIA------GHTAGASCDEVKSPQE-VNNVGGGAPVTAPYTVT-FASNYHDRVDTKLHRAYPEFLQYHLIHETLRGKEKIEGYEVYKDRRDDSIVAFARLGKLLSGHPDIIHGGSIAALLDNTMGVAFFAANKGNGFTANLTINYKRPIICGTEIKVLARVERFEGRKVFLRAEIRDAKDEAVLYTEATSLFITSQSPLLTGPKKVDIS 274          
BLAST of NO05G05420 vs. NCBI_GenBank
Match: XP_004349120.1 (hypothetical protein CAOG_02370 [Capsaspora owczarzaki ATCC 30864] >KJE91205.1 hypothetical protein CAOG_002370 [Capsaspora owczarzaki ATCC 30864])

HSP 1 Score: 96.3 bits (238), Expect = 1.700e-16
Identity = 51/129 (39.53%), Postives = 73/129 (56.59%), Query Frame = 0
Query:  147 LIHETLRGKEKIDGYEVYKDRRDDSIVAYARLGKLLSGHPDIIHGGSIAALLDNTMGVAFFAAKRGNGFTANLTINYKRPITCGTEVKVLARVEKVEGRKVFLRAEIRDAKDEAILYTEAKSLFITSQS 276
            L  E L G  K +  E+Y +  D   V   +LG  + GHPD IHGG+I    D T+G  F     G G+TANL INY++P+     + V A V++VEGRKVFL A + D     ++Y +A +LF+  ++
Sbjct:  228 LTFEALYGPGKFENVEMYHNPTDLQFVGMFKLGNRICGHPDTIHGGAIGVAFDETLGWLFMLT-AGVGYTANLNINYRKPLPALHTIVVKAHVDRVEGRKVFLHATLEDL--HGLVYADAAALFVVPRN 353          
BLAST of NO05G05420 vs. NCBI_GenBank
Match: ORX95267.1 (Thioesterase/thiol ester dehydrase-isomerase [Basidiobolus meristosporus CBS 931.73])

HSP 1 Score: 96.3 bits (238), Expect = 1.700e-16
Identity = 53/127 (41.73%), Postives = 79/127 (62.20%), Query Frame = 0
Query:  147 LIHETLRGKEKID-GYEVYKDRRDDSIVAYARLGKLLSGHPDIIHGGSIAALLDNTMGVAFFAAKRG-NGFTANLTINYKRPITCGTEVKVLARVEKVEGRKVFLRAEIRDAKDEAILYTEAKSLFI 272
            L   TL+GK+K+     ++++ +++S+V    LGK L GH  IIHGG +A LLD  +    F +  G NG TANL INY++P+     V + ARV KVEGRK ++ A+++D K   ++  EA +LFI
Sbjct:  126 LTRYTLKGKDKLALDPVIFRNDKENSLVIVTHLGKALCGHKGIIHGGLLATLLDEGLAATVFPSMPGKNGVTANLNINYRKPVLADQFVSINARVTKVEGRKGWVEAQVKDMKGNVLV--EASALFI 250          
BLAST of NO05G05420 vs. NCBI_GenBank
Match: XP_003479092.1 (LOW QUALITY PROTEIN: acyl-coenzyme A thioesterase THEM4 [Cavia porcellus])

HSP 1 Score: 92.8 bits (229), Expect = 1.800e-15
Identity = 60/150 (40.00%), Postives = 86/150 (57.33%), Query Frame = 0
Query:  132 DTKLHRAYPEFLKFHLIHETLRGKEKI-----DG----YEVYKDRRDDSIVAYARLGKLLSGHPDIIHGGSIAALLDNTMGVAFFAAKRGNGFTANLTINYKRPITCGTEVKVLARVEKVEGRKVFLRAEIRDAKDEAILYTEAKSLFIT 273
            D K H   P+ LK     E + G +       DG    Y ++ +  ++ +V + + G  L G P   HGG+IAA++D T+G   F A RG   TANL INYKRPI   + V + ++++KVEGRKVF+  ++R  K+E  LY+EA  LFIT
Sbjct:   86 DFKTHYVDPKLLK----EEQMPGAQLFTRSFEDGLGFEYVMFCNDVENRVVCFFQGGPHLQGMPGFTHGGAIAAIIDATVGTCAFRA-RGIVMTANLNINYKRPIPLXSVVVINSQLDKVEGRKVFVSCDVRSVKEET-LYSEATGLFIT 229          
BLAST of NO05G05420 vs. NCBI_GenBank
Match: XP_007428866.1 (PREDICTED: acyl-coenzyme A thioesterase THEM4 isoform X1 [Python bivittatus] >XP_015743660.1 PREDICTED: acyl-coenzyme A thioesterase THEM4 isoform X1 [Python bivittatus])

HSP 1 Score: 91.7 bits (226), Expect = 4.100e-15
Identity = 63/180 (35.00%), Postives = 93/180 (51.67%), Query Frame = 0
Query:  105 MREDGGKGEATSSATCNPSLFEHHDRVDTKLHRAYPEFLKFHLIHETLRGKEKIDGYEVYKDRRDDSIVAYARLGKLLSGHPDIIHGGSIAALLDNTMGVAFFAAKRGNGFTANLTINYKRPITCGTEVKVLARVEKVEGRKVFLRAEIRDAKDEAILYTEAKSLFITSQSPLLKGPKKI 285
            M +DG   +  S  +      E H+R + +  R     L    I E   G E    Y ++ +  +  +V  ++LG  L G     HGGS+A LLD+T+G       R    TANL+INYK P+  G+ V   +R++K+EGRK+F+  EIR A D  IL+ EA +LFI  +S +L   K +
Sbjct:   71 MTKDGSWQKLPSYRSFYEHFPEGHERCELQKRR---NRLFLRSIDEEGMGFE----YVMFLNATEKRMVCLSQLGPYLEGPSGFAHGGSLATLLDSTLGGCAICVNR-RVLTANLSINYKNPVALGSVVLAESRLDKIEGRKIFVSGEIRSA-DRQILHAEATALFIQLESSILPQEKTV 241          
BLAST of NO05G05420 vs. NCBI_GenBank
Match: OXV11181.1 (hypothetical protein Egran_01054 [Elaphomyces granulatus])

HSP 1 Score: 91.7 bits (226), Expect = 4.100e-15
Identity = 58/148 (39.19%), Postives = 83/148 (56.08%), Query Frame = 0
Query:  131 VDTKLHRAYPEFLKFH-LIHETLRGKEKI--DGYEVYKDRRDDSIVAYARLGKLLSGHPDIIHGGSIAALLDNTMGVAFFAA-KRGNGFTANLTINYKRPITCGTEVKVLARVEKVEGRKVFLRAEIR---DAKDEAILYTEAKSLFI 272
            V+++ H   PE L+   LI  TL G  KI    Y  + ++   S V+   LG  +SGHPDI+HGG +A +LD  MG+  F A   G G TANL I+YKRP+   T   + A   KV GRK ++   I    +  +E +++ EAK+LF+
Sbjct:  140 VESRPHLKIPEGLRSRTLIAGTLAGSNKIVVPPYS-WSEKAGKSTVSIYYLGSDISGHPDIVHGGLLATILDEGMGICCFGALPSGFGVTANLNIDYKRPVPTNTYAVLKAETVKVAGRKAWVEGRIESLPEKGEEPVVFVEAKALFV 286          
BLAST of NO05G05420 vs. NCBI_GenBank
Match: ORX92776.1 (Thioesterase/thiol ester dehydrase-isomerase, partial [Basidiobolus meristosporus CBS 931.73])

HSP 1 Score: 90.9 bits (224), Expect = 7.000e-15
Identity = 50/131 (38.17%), Postives = 79/131 (60.31%), Query Frame = 0
Query:  147 LIHETLRGKEKIDGYE-VYKDRRDDSIVAYARLGKLLSGHPDIIHGGSIAALLDNTMGVAFFAAKRGN-GFTANLTINYKRPITCGTEVKVLARVEKVEGRKVFLRAEIRDAKDEAILYTEAKSLFITSQS 276
            L+  TL G+ K++    +++   +  +V    LG  L GH  I+HGG +A+LLD+T+G        G+ GFTANL+INY+RP      V +   VEKVEGRK F++  + D KD  ++  ++++LFI+ +S
Sbjct:    2 LLWNTLHGEGKLELEPLIFRSADEKKVVMIVYLGSKLCGHKGIVHGGMLASLLDDTLGRTIIPNLPGSVGFTANLSINYRRPTFADDFVVIKTEVEKVEGRKGFVKGHVEDTKDNVLV--DSQALFISPKS 130          
BLAST of NO05G05420 vs. NCBI_GenBank
Match: XP_008618768.1 (hypothetical protein SDRG_14419 [Saprolegnia diclina VS20] >EQC27835.1 hypothetical protein SDRG_14419 [Saprolegnia diclina VS20])

HSP 1 Score: 89.7 bits (221), Expect = 1.600e-14
Identity = 47/126 (37.30%), Postives = 70/126 (55.56%), Query Frame = 0
Query:  146 HLIHETLRGKEKIDGYEVYKDRRDDSIVAYARLGKLLSGHPDIIHGGSIAALLDNTMGVAFFAAKRGNGFTANLTINYKRPITCGTEVKVLARVEKVEGRKVFLRAEIRDAKDEAILYTEAKSLFI 272
            H IH     + K + + VY D      ++    G+ L GH  I+HGG I+ + D   G   +      GFTANL +N+++P+   T   V   +E++EGRKV+L+A+++DA  E   YTEA SLFI
Sbjct:   63 HYIHSLSDIQGKYEEFMVYGDVAKTQTISLVHFGEKLCGHTGIVHGGCISTVCDELFGWTMYWIMDKAGFTANLNVNFRKPLPAQTLAIVTTELERIEGRKVYLKAKVQDA--EGNTYTEATSLFI 186          
BLAST of NO05G05420 vs. NCBI_GenBank
Match: ORX47679.1 (Thioesterase/thiol ester dehydrase-isomerase [Hesseltinella vesiculosa])

HSP 1 Score: 89.7 bits (221), Expect = 1.600e-14
Identity = 52/131 (39.69%), Postives = 77/131 (58.78%), Query Frame = 0
Query:  151 TLRGKEK-IDGYEVYKDRRDDSIVAYARLGKLLSGHPDIIHGGSIAALLDNTMG-VAFFAAKRGNGFTANLTINYKRPITCGTEVKVLARVEKVEGRKVFLRAEIRDAKDEAILYTEAKSLFITSQSPLLK 280
            +LRGK K +    V+ ++    +     LGK L GH  I+HGG +A LLD  +  VA  +     GFTANL I+Y+RP+T    V +  +++KVEGRK F+RA I D  +    + EA SL+++ +SPL +
Sbjct:   59 SLRGKGKLVVSPAVFYNKDQTEVTVVTHLGKQLCGHDGIVHGGMLATLLDEVLAFVAMPSLPNKVGFTANLNIDYRRPVTSHQWVVLKGQLDKVEGRKAFVRAWIEDL-EGTTKFVEATSLYVSPKSPLAR 188          
The following BLAST results are available for this feature:
BLAST of NO05G05420 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes)
Total hits: 20
Match NameE-valueIdentityDescription
EWM27855.11.300e-7769.70thioesterase superfamily member 4 [Nannochloropsis... [more]
XP_005852768.13.800e-7769.70thioesterase superfamily member 4 [Nannochloropsis... [more]
XP_004349120.11.700e-1639.53hypothetical protein CAOG_02370 [Capsaspora owczar... [more]
ORX95267.11.700e-1641.73Thioesterase/thiol ester dehydrase-isomerase [Basi... [more]
XP_003479092.11.800e-1540.00LOW QUALITY PROTEIN: acyl-coenzyme A thioesterase ... [more]
XP_007428866.14.100e-1535.00PREDICTED: acyl-coenzyme A thioesterase THEM4 isof... [more]
OXV11181.14.100e-1539.19hypothetical protein Egran_01054 [Elaphomyces gran... [more]
ORX92776.17.000e-1538.17Thioesterase/thiol ester dehydrase-isomerase, part... [more]
XP_008618768.11.600e-1437.30hypothetical protein SDRG_14419 [Saprolegnia dicli... [more]
ORX47679.11.600e-1439.69Thioesterase/thiol ester dehydrase-isomerase [Hess... [more]

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Relationships

This gene is member of the following syntenic_region feature(s):

Feature NameUnique NameSpeciesType
nonsL085nonsL085Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ncniR027ncniR027Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ngnoR117ngnoR117Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region


This gene is orthologous to the following gene feature(s):

Feature NameUnique NameSpeciesType
NSK003301NSK003301Nannochloropsis salina (N. salina CCMP1776)gene


The following polypeptide feature(s) derives from this gene:

Feature NameUnique NameSpeciesType
NO05G05420.2NO05G05420.2-proteinNannochloropsis oceanica (N. oceanica IMET1)polypeptide
NO05G05420.1NO05G05420.1-proteinNannochloropsis oceanica (N. oceanica IMET1)polypeptide


The following gene feature(s) are orthologous to this gene:

Feature NameUnique NameSpeciesType
jgi.p|Nanoce1779_2|675911gene_11940Nannochloropsis oceanica (N. oceanica CCMP1779)gene
Naga_100219g2gene2706Nannochloropsis gaditana (N. gaditana B-31)gene


The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
NO05G05420.2NO05G05420.2Nannochloropsis oceanica (N. oceanica IMET1)mRNA
NO05G05420.1NO05G05420.1Nannochloropsis oceanica (N. oceanica IMET1)mRNA


Sequences
The following sequences are available for this feature:

gene sequence

>NO05G05420 ID=NO05G05420|Name=NO05G05420|organism=Nannochloropsis oceanica|type=gene|length=1569bp
GGTTGCGTGTGTGTAGGTCGAGCATGTTGTGTGAGTCTTGCATCCCATCT
CATTTCTTCTATGCATGCGTTCGGTGCGTATCGTGATGATGCGGGCTCGC
AATCCATTTTTCAAGCATTGAGTACATACGAGTCCCAATGTTTTATACAC
AACCACAGGCCCTGTGCTCATGACGCCTTTGGCCTTCACGGTGCTCGGCA
AGCTTGGTGGCACGTTGACTTTTGCTTGTGTACGACGGAGGCTTTATCAC
TTGTTACGGCGGGCAACTTTGTCCTCCCATTATCAGGTCACTCGGCCTTA
CGGTCACAGCAATTCCGGCTGTTCACATAGCACTACCACACTTAGAACCA
GCTTCCCAGTCCTCTTTGCGCAATTGGCAGCAGCCACTGCTGCCGTCGTC
GCTGCCATTTCCCTGCCGTCGCCTAGTCTATGCGAGACGGCCCACGCCGG
GACTGAGGAGAGACGAGGTGAGAGGAAGGCAATGAGGGAGGATGGTGGAA
AAGGCGAGGCCACCTCGTCTGCTACATGCAATCCATCCTTATTCGAACAT
CATGATCGCGTCGACACCAAGCTGCATCGGGCCTATCCTGAATTCCTGAA
Ggttggttattggcgggatggatgttaatgaagtgctcgcccctcctcct
ttcgtttctccgttatatggactctgctcctttggttaagctgggcaaga
tacgtgcatggcacgtcatatcttcgtattctcatgcttctcagtgtttg
acgctatcatagccacttttgaaagagcatcttccctcctcccttctccc
tcctcatcttttgcatcctcctaccactttcttcccccaccacccagTTC
CACCTTATCCACGAGACGCTCCGAGGCAAAGAGAAAATTGATGGCTACGA
AGTTTACAAAGACAGGCGGGATGATTCAATTGTGGCGTATGCTCGCCTTG
GCAAACTGCTGAGCGGACACCCCGACATAATCCACGGAGGGTCCATTGCG
GCTTTGCTGGACAATACCATGGGAGTTGCCTTTTTCGCCGCCAAGGTGAG
TGATAATCATTGACAAGGGAGAAAGAGAAGAAGATTTGAACGAAGAGCTG
GGATGCCGTTTGCAAAACGGATGCTTTGGATGCGTGAACGAAGTGTTTGC
TTTATTGGACGTGAGAAGTGAGTGCAGCTAGTCGTCTTTTCACCTCTACC
CTCTACGTCTTCGGCTTGCGACCCTCCATCTGCTACTCAAATAACAGATA
ATTCGAAGCCTAAGTGCACGTTCTCATATTTTCTATTCATCTTCACGATT
CATACATAGCGTGGCAATGGTTTTACAGCAAATCTCACCATCAACTACAA
GCGACCCATCACGTGTGGCACCGAAGTCAAAGTTTTAGCTCGAGTAGAGA
AGGTGGAAGGGCGCAAGGTCTTCTTGCGGGCCGAGATTCGAGACGCTAAG
GATGAGGCTATCCTCTACACTGAAGCCAAATCCCTCTTCATCACGTCTCA
AAGTCCTTTATTGAAGGGCCCAAAGAAAATTGATATTAGCTAGCTATATT
GGTAGGAAGATAAGACAAG
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protein sequence of NO05G05420.2

>NO05G05420.2-protein ID=NO05G05420.2-protein|Name=NO05G05420.2|organism=Nannochloropsis oceanica|type=polypeptide|length=216bp
MTPLAFTVLGKLGGTLTFACVRRRLYHLLRRATLSSHYQVTRPYGHSNSG
CSHSTTTLRTSFPVLFAQLAAATAAVVAAISLPSPSLCETAHAGTEERRG
ERKAMREDGGKGEATSSATCNPSLFEHHDRVDTKLHRAYPEFLKFHLIHE
TLRGKEKIDGYEVYKDRRDDSIVAYARLGKLLSGHPDIIHGGSIAALLDN
TMGVAFFAAKVSDNH*
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protein sequence of NO05G05420.1

>NO05G05420.1-protein ID=NO05G05420.1-protein|Name=NO05G05420.1|organism=Nannochloropsis oceanica|type=polypeptide|length=288bp
MTPLAFTVLGKLGGTLTFACVRRRLYHLLRRATLSSHYQVTRPYGHSNSG
CSHSTTTLRTSFPVLFAQLAAATAAVVAAISLPSPSLCETAHAGTEERRG
ERKAMREDGGKGEATSSATCNPSLFEHHDRVDTKLHRAYPEFLKFHLIHE
TLRGKEKIDGYEVYKDRRDDSIVAYARLGKLLSGHPDIIHGGSIAALLDN
TMGVAFFAAKRGNGFTANLTINYKRPITCGTEVKVLARVEKVEGRKVFLR
AEIRDAKDEAILYTEAKSLFITSQSPLLKGPKKIDIS*
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Synonyms
Publications