NO05G00960, NO05G00960 (gene) Nannochloropsis oceanica

Overview
NameNO05G00960
Unique NameNO05G00960
Typegene
OrganismNannochloropsis oceanica (N. oceanica IMET1)
Sequence length1025
Alignment locationchr5:292660..293684 +

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Properties
Property NameValue
Description6,7-dimethyl-8-ribityllumazine synthase
Mutants
Expression

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
chr5genomechr5:292660..293684 +
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
PRJNA7699582024-08-13
GSE1786722023-12-15
PRJNA9336932023-10-26
PRJNA9434492023-07-19
PXD0160542021-01-14
PXD0166992021-01-08
GSE1499042020-10-10
NoIMET1_WT_HS2020-09-29
PXD0100302020-03-16
GSE1396152020-03-11
PRJNA2413822020-03-11
BLASTP analysis of N. oceanica IMET1 genes2019-07-11
InterPro analysis for N. oceanica IMET1 genes2019-07-11
GO annotation for IMET1v2 genes2019-07-10
PXD0087212019-04-30
PRJNA1821802019-04-25
Gene prediction for N. oceanica IMET12017-10-24
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:00009066,7-dimethyl-8-ribityllumazine synthase activity
GO:0016740transferase activity
Vocabulary: Cellular Component
TermDefinition
GO:0009349riboflavin synthase complex
GO:0016020membrane
GO:0005623cell
Vocabulary: Biological Process
TermDefinition
GO:0009231riboflavin biosynthetic process
GO:0009987cellular process
GO:0009058biosynthetic process
Vocabulary: INTERPRO
TermDefinition
IPR036467LS/RS_sf
IPR034964LS
IPR002180DMRL_synthase
Homology
BLAST of NO05G00960 vs. NCBI_GenBank
Match: EWM28253.1 (dimethyl-8-ribityllumazine synthase [Nannochloropsis gaditana])

HSP 1 Score: 367.5 bits (942), Expect = 3.200e-98
Identity = 186/234 (79.49%), Postives = 206/234 (88.03%), Query Frame = 0
Query:    1 MLRFPSLVATTLLLLACVLRQATAFVMPRPALPTMSSNSRMLIAPLSMSKDLVGFGEDLDGSACRIGIVKTRWNPEIVDSLYEGVRAALKETGVKDANIYDTTVPGSFELPLAARFLAMSRQCDVVITIGCLIKGDTMHFEYIAEAVTKGLMDIQLQTTIPVIFGLLTVLNKDQAIVRSKGENNHGISWGKTAVEMALLRTAAMAKGPNRATLPLTEKPGDTPDEKKQERQFGF 235
            M+R P++VA     +AC+L+   AFV PRP L + +  S M + PL MSKDLVGFG+DLDGS CRIGIVKTRWNPEIVDSLYEGVRAAL ETGVKD N+YDTTVPGSFELPLAARFLA+SRQCDVVITIGCLIKG+TMHFEYIAEAVTKGLMDIQLQTTIPVIFG+LTVL ++QA+ RSKGENNHGISWGKTAVEMALLR AA+AKGPNRATLPLTEK  DTP+EKKQ +QFGF
Sbjct:   28 MVRLPTIVA-----VACLLQGIAAFVTPRPVLLSPARPS-MRVNPLMMSKDLVGFGDDLDGSTCRIGIVKTRWNPEIVDSLYEGVRAALNETGVKDENVYDTTVPGSFELPLAARFLALSRQCDVVITIGCLIKGETMHFEYIAEAVTKGLMDIQLQTTIPVIFGVLTVLTEEQAVARSKGENNHGISWGKTAVEMALLRNAALAKGPNRATLPLTEKLEDTPEEKKQSKQFGF 255          
BLAST of NO05G00960 vs. NCBI_GenBank
Match: CBN77125.1 (6,7-dimethyl-8-ribityllumazine synthase [Ectocarpus siliculosus])

HSP 1 Score: 206.5 bits (524), Expect = 9.300e-50
Identity = 107/207 (51.69%), Postives = 143/207 (69.08%), Query Frame = 0
Query:   14 LLACVLRQATAFVMPRPALPTMSS-------------NSRMLIAPLSMSKDLVGFGEDLDGSACRIGIVKTRWNPEIVDSLYEGVRAALKETGVKDANIYDTTVPGSFELPLAARFLAMSRQCDVVITIGCLIKGDTMHFEYIAEAVTKGLMDIQLQTTIPVIFGLLTVLNKDQAIVRSKGENNHGISWGKTAVEMALLRTAAMAKG 208
            L AC  R A+AFV P  ++  + +             +SR   A   M+ + V FGE LDG+  R+GI+ TRWN EIV+ L +GV+ +LKE  V ++NI++T VPG+FELPLAARFL++S+  D V+ +GCLIKGDTMHFEYIAE+  KGLMD+ +QT+ PV+ G+LT   ++QAI RS G+NNHG+ WGKTAVEM LLR +A   G
Sbjct:   14 LFAC--RTASAFVAPAASIRALRNAPAGGASTTSTYGSSRGAPATRMMAAEGVTFGE-LDGTDVRVGIISTRWNEEIVNRLTDGVKESLKECNVTESNIFETKVPGAFELPLAARFLSLSQTVDAVVCVGCLIKGDTMHFEYIAESTCKGLMDVSIQTSTPVVLGVLTCTEEEQAIKRSTGDNNHGVDWGKTAVEMGLLRMSATGVG 217          
BLAST of NO05G00960 vs. NCBI_GenBank
Match: GAX17980.1 (6,7-dimethyl-8-ribityllumazine synthase [Fistulifera solaris])

HSP 1 Score: 203.0 bits (515), Expect = 1.000e-48
Identity = 104/181 (57.46%), Postives = 131/181 (72.38%), Query Frame = 0
Query:   24 AFVMPRPALPTMSSNSRMLIAPLSMSKDLVGFGEDLDGSACRIGIVKTRWNPEIVDSLYEGVRAALKETGVKDANIYDTTVPGSFELPLAARFLAMSRQCDVVITIGCLIKGDTMHFEYIAEAVTKGLMDIQLQTTIPVIFGLLTVLNKDQAIVRSKGENNHGISWGKTAVEMALLRTAAM 205
            AFV+  P L   SS + +  A   +S D      +LDGS  RIGI+KTRWN E V++L  G++  LKE  V D NI++T+VPGSFELP+AARFLA++  CD +IT G LIKGDTMHFE+IA+AV KG+M+I LQT+ PV+FG+LT  N+ Q   RS G+NNHG  WGKTAVEMALLRT A+
Sbjct:    7 AFVVQTPLL---SSKTALQAADSDVSFD------NLDGSQMRIGIIKTRWNEEHVNNLVAGIKKGLKECKVSDTNIFETSVPGSFELPMAARFLALTGTCDAIITTGVLIKGDTMHFEFIADAVAKGIMNIGLQTSTPVVFGVLTCQNEQQVKDRSTGDNNHGYDWGKTAVEMALLRTEAL 178          
BLAST of NO05G00960 vs. NCBI_GenBank
Match: GAX17543.1 (6,7-dimethyl-8-ribityllumazine synthase [Fistulifera solaris])

HSP 1 Score: 193.4 bits (490), Expect = 8.200e-46
Identity = 90/140 (64.29%), Postives = 112/140 (80.00%), Query Frame = 0
Query:   65 RIGIVKTRWNPEIVDSLYEGVRAALKETGVKDANIYDTTVPGSFELPLAARFLAMSRQCDVVITIGCLIKGDTMHFEYIAEAVTKGLMDIQLQTTIPVIFGLLTVLNKDQAIVRSKGENNHGISWGKTAVEMALLRTAAM 205
            RIGI+KTRWN E V++L  G++  LKE  V D NI++T+VPGSFELP+AARFLA++  CD +IT G LIKGDTMHFE+IA+AV KG+M+I LQT+ PV+FG+LT  N+ Q   RS G+NNHG  WGKTAVEMALLRT A+
Sbjct:    2 RIGIIKTRWNEEHVNNLVAGIKKGLKECKVSDTNIFETSVPGSFELPMAARFLALTGTCDAIITTGVLIKGDTMHFEFIADAVAKGIMNIGLQTSTPVVFGVLTCQNEQQVKDRSTGDNNHGYDWGKTAVEMALLRTEAL 141          
BLAST of NO05G00960 vs. NCBI_GenBank
Match: OEU22250.1 (Lumazine synthase, partial [Fragilariopsis cylindrus CCMP1102])

HSP 1 Score: 188.3 bits (477), Expect = 2.600e-44
Identity = 85/141 (60.28%), Postives = 112/141 (79.43%), Query Frame = 0
Query:   58 DLDGSACRIGIVKTRWNPEIVDSLYEGVRAALKETGVKDANIYDTTVPGSFELPLAARFLAMSRQCDVVITIGCLIKGDTMHFEYIAEAVTKGLMDIQLQTTIPVIFGLLTVLNKDQAIVRSKGENNHGISWGKTAVEMAL 199
            DLDGS  RIGI++TRWN E V +L +G R ALKE  VK+ NI++T++PG++ELPL+ARFLA+S   D ++T G LIKG+TMHFEYI++AV+ GLM +QLQTT P+++G+L  L++ Q   RS G+NNHG  WGKTAVEMAL
Sbjct:    1 DLDGSDIRIGIIRTRWNDEHVTNLVDGCRKALKECNVKEENIFETSIPGAYELPLSARFLALSGTVDAIVTAGVLIKGETMHFEYISDAVSSGLMSVQLQTTCPIVYGVLNCLDEGQVKSRSSGDNNHGEDWGKTAVEMAL 141          
BLAST of NO05G00960 vs. NCBI_GenBank
Match: XP_002179359.1 (predicted protein, partial [Phaeodactylum tricornutum CCAP 1055/1] >EEC49182.1 predicted protein, partial [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 187.6 bits (475), Expect = 4.500e-44
Identity = 86/141 (60.99%), Postives = 114/141 (80.85%), Query Frame = 0
Query:   58 DLDGSACRIGIVKTRWNPEIVDSLYEGVRAALKETGVKDANIYDTTVPGSFELPLAARFLAMSRQCDVVITIGCLIKGDTMHFEYIAEAVTKGLMDIQLQTTIPVIFGLLTVLNKDQAIVRSKGENNHGISWGKTAVEMAL 199
            DLDGSA RIGI++TRWN E V +L +G++  L+   V++ NI++T+VPG+FELP+AARFLA+S   D +IT G LIKG+TMHFEYI++AV KG+M+I LQT+ PVIFG+LT L++ Q   RS G++NHG+ WGKTAVEMAL
Sbjct:    3 DLDGSAMRIGIIRTRWNDEHVTNLVDGIKEGLRACQVEEDNIFETSVPGAFELPMAARFLALSGTVDAIITTGVLIKGETMHFEYISDAVAKGIMNIGLQTSTPVIFGVLTCLDEAQVQARSSGDSNHGVDWGKTAVEMAL 143          
BLAST of NO05G00960 vs. NCBI_GenBank
Match: CEM26950.1 (unnamed protein product [Vitrella brassicaformis CCMP3155])

HSP 1 Score: 187.6 bits (475), Expect = 4.500e-44
Identity = 113/255 (44.31%), Postives = 149/255 (58.43%), Query Frame = 0
Query:    7 LVATTLLLLACVLRQATAFVMPR-PALPTMSSN---------SRMLIAPLSMSKDLVG--------FGED---LDGSACRIGIVKTRWNPEIVDSLYEGVRAALKETGVKDANIYDTTVPGSFELPLAARFLAMSRQCDVVITIGCLIKGDTMHFEYIAEAVTKGLMDIQLQTTIPVIFGLLTVLNKDQAIVRSKGENNHGISWGKTAVEMALLRTAAMAK-------------GPNRATLPLTEKPGDTPDEKK 228
            L++++ LLL   L  A +F  P  P LP  S              L  P +  +D+           G D   L+GS  +IGI+KTRWN +IVD+L +GV+  L E  V   NI +  VPGS+ELPLA RFLA+S Q D +++IG LIKGDT+HFEYIAE+V+ GLM++QLQT  P +FG+LT LN +QA  RS G +NHGISWGKTAVEM++LR  A+ +              P      L   P  TPD+K+
Sbjct:    4 LLSSSALLLLHALPSAVSFYSPTLPPLPASSVRRLRAGTRHVRASLFMPSAWQRDVASKLRMVAPREGVDFPPLNGSDVKIGIIKTRWNKDIVDNLVKGVKEGLTECSVPAENILEIEVPGSYELPLATRFLALSNQVDAIVSIGVLIKGDTLHFEYIAESVSNGLMNVQLQTMTPTVFGVLTCLNLNQAKERSSGPDNHGISWGKTAVEMSVLRRYALGRYRKGSLGFVPTEESPEGEPNNLPATPWPTPDKKR 258          
BLAST of NO05G00960 vs. NCBI_GenBank
Match: XP_005772319.1 (hypothetical protein EMIHUDRAFT_444779 [Emiliania huxleyi CCMP1516] >XP_005783542.1 hypothetical protein EMIHUDRAFT_434395 [Emiliania huxleyi CCMP1516] >EOD19890.1 hypothetical protein EMIHUDRAFT_444779 [Emiliania huxleyi CCMP1516] >EOD31113.1 hypothetical protein EMIHUDRAFT_434395 [Emiliania huxleyi CCMP1516])

HSP 1 Score: 187.2 bits (474), Expect = 5.900e-44
Identity = 93/179 (51.96%), Postives = 128/179 (71.51%), Query Frame = 0
Query:   30 PALPTMSSNSRMLIAPLSMSKDLVGFGEDLDGSACRIGIVKTRWNPEIVDSLYEGVRAALKETGVKDANIYDTTVPGSFELPLAARFLAMSRQCDVVITIGCLIKGDTMHFEYIAEAVTKGLMDIQLQTTIPVIFGLLTVLNKDQAIVRSKGENNHGISWGKTAVEMALLRTAAMAKGP 209
            P++P   S +R   A + M+ D   FG DLDGS  RIGI+K RW+PE+ DSL  G + AL   GVK+ NI +T VPGS+ELPLAAR++A+S   D ++ +G LIKGDT HFE I+++V+ GLM + +QT +P++FG+LT ++++QA VRS G  NHG  WG+ AVEMALLR +A+  GP
Sbjct:   16 PSVPATGSRAR--AAAVRMAGDGPSFG-DLDGSTARIGIIKARWHPEMSDSLIAGAKEALAACGVKEENIVETEVPGSYELPLAARYMALSGSVDAILPVGILIKGDTYHFEAISDSVSSGLMSVGMQTGVPILFGVLTCMSEEQARVRSIGPGNHGPDWGRAAVEMALLRQSAVG-GP 190          
BLAST of NO05G00960 vs. NCBI_GenBank
Match: XP_005827094.1 (hypothetical protein GUITHDRAFT_154245 [Guillardia theta CCMP2712] >EKX40114.1 hypothetical protein GUITHDRAFT_154245 [Guillardia theta CCMP2712])

HSP 1 Score: 168.3 bits (425), Expect = 2.800e-38
Identity = 78/154 (50.65%), Postives = 108/154 (70.13%), Query Frame = 0
Query:   59 LDGSACRIGIVKTRWNPEIVDSLYEGVRAALKETGVKDANIYDTTVPGSFELPLAARFLAMSRQCDVVITIGCLIKGDTMHFEYIAEAVTKGLMDIQLQTTIPVIFGLLTVLNKDQAIVRSKGENNHGISWGKTAVEMALLRTAAMAKGPNRAT 213
            LDG   R+GIV TRWN E+V+ L +G RA LKE  V+D+N+ +  VPGS+ELPLA R++A +++ D +I IG L+KG+T H++ I +A T  LM + L+T IPV+ G+L     +QA  R+ G+NNHG+ WGKTA+EMA LR   M KGP  A+
Sbjct:   68 LDGKGMRVGIVSTRWNAEVVNKLKDGARAGLKECQVEDSNVVEFEVPGSWELPLACRYMAQTQKVDAIIAIGVLVKGETDHYDMIKDAATDALMMLGLETGIPVLNGILGCHTMEQAEARATGDNNHGVWWGKTAIEMATLRATQMGKGPIAAS 221          
BLAST of NO05G00960 vs. NCBI_GenBank
Match: XP_004365556.1 (6,7-dimethyl-8-ribityllumazine synthase subunit [Capsaspora owczarzaki ATCC 30864] >KJE89156.1 6,7-dimethyl-8-ribityllumazine synthase subunit [Capsaspora owczarzaki ATCC 30864])

HSP 1 Score: 164.5 bits (415), Expect = 4.100e-37
Identity = 82/150 (54.67%), Postives = 106/150 (70.67%), Query Frame = 0
Query:   59 LDGSACRIGIVKTRWNPEIVDSLYEGVRAALKETGVKDANIYDTTVPGSFELPLAARFLAMS--RQCDVVITIGCLIKGDTMHFEYIAEAVTKGLMDIQLQTTIPVIFGLLTVLNKDQAIVR-----SKGENNHGISWGKTAVEMALLRT 202
            L+G   RIGIV+ RWNP ++D+L  G RAAL E+GV +++I +  VPGS+ELP AA  L  S   + D V+ +GCLIKG+TMHFEYI EAVT+G+M + L +  PV+FG+L  L  DQA+ R     S  ++NHGI WGK AVEMALLRT
Sbjct:   13 LNGRGLRIGIVRARWNPTVIDALNAGCRAALLESGVAESDIVEVMVPGSYELPFAASVLLNSADAKVDAVVCLGCLIKGETMHFEYICEAVTQGIMRLNLDSGKPVMFGVLACLTMDQALARAGLGDSPSKHNHGIDWGKGAVEMALLRT 162          
The following BLAST results are available for this feature:
BLAST of NO05G00960 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes)
Total hits: 20
Match NameE-valueIdentityDescription
EWM28253.13.200e-9879.49dimethyl-8-ribityllumazine synthase [Nannochlorops... [more]
CBN77125.19.300e-5051.696,7-dimethyl-8-ribityllumazine synthase [Ectocarpu... [more]
GAX17980.11.000e-4857.466,7-dimethyl-8-ribityllumazine synthase [Fistulife... [more]
GAX17543.18.200e-4664.296,7-dimethyl-8-ribityllumazine synthase [Fistulife... [more]
OEU22250.12.600e-4460.28Lumazine synthase, partial [Fragilariopsis cylindr... [more]
XP_002179359.14.500e-4460.99predicted protein, partial [Phaeodactylum tricornu... [more]
CEM26950.14.500e-4444.31unnamed protein product [Vitrella brassicaformis C... [more]
XP_005772319.15.900e-4451.96hypothetical protein EMIHUDRAFT_444779 [Emiliania ... [more]
XP_005827094.12.800e-3850.65hypothetical protein GUITHDRAFT_154245 [Guillardia... [more]
XP_004365556.14.100e-3754.676,7-dimethyl-8-ribityllumazine synthase subunit [C... [more]

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Relationships

This gene is member of the following syntenic_region feature(s):

Feature NameUnique NameSpeciesType
nonsL087nonsL087Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
nonsL086nonsL086Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
nonsL084nonsL084Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ncniR092ncniR092Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ngnoR122ngnoR122Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ngnoR119ngnoR119Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ngnoR118ngnoR118Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region


This gene is orthologous to the following gene feature(s):

Feature NameUnique NameSpeciesType
NSK003788NSK003788Nannochloropsis salina (N. salina CCMP1776)gene


The following polypeptide feature(s) derives from this gene:

Feature NameUnique NameSpeciesType
NO05G00960.1NO05G00960.1-proteinNannochloropsis oceanica (N. oceanica IMET1)polypeptide


The following gene feature(s) are orthologous to this gene:

Feature NameUnique NameSpeciesType
Naga_100004g9gene2274Nannochloropsis gaditana (N. gaditana B-31)gene


The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
NO05G00960.1NO05G00960.1Nannochloropsis oceanica (N. oceanica IMET1)mRNA


Sequences
The following sequences are available for this feature:

gene sequence

>NO05G00960 ID=NO05G00960|Name=NO05G00960|organism=Nannochloropsis oceanica|type=gene|length=1025bp
ATGTTGCGCTTCCCATCCCTCGTCGCGACCACCCTTCTCCTGCTAGCATG
CGTGCTAAGACAGGCAACCGCGTTCGTTATGCCACGGCCAGCACTTCCGA
CCATGAGCAGCAACAGCCGTATGgtgcgtagcttagtttttacgggcttg
ttcgtcctcccaaatctcgcctacccttttatttccatagaacaaaccga
cgcacacacaccaactgaacccccttcaacgactacgccacagCTAATCG
CCCCCCTTTCCATGTCGAAGGACTTGGTTGGCTTTGGTGAAGATCTGGAC
GGCTCCGCCTGCCGCATCGGCATCGTCAAGACACGTTGGAACCCCGAAAT
CGTAGACTCCCTTTACGAAGGCGTGCGCGCTGCGCTGAAAGAAACAGGGG
TCAAGGATGCCAACATCTACGATACCACCGTTCCCGGCTCTTTTGAGTTG
CCGTTGGCCGCGCGCTTTCTGGCGATGTCGCGGCAGTGTGACGTGGTTAT
TACGATTGGGTGCTTGATCAAGGGAGACACCATGCATTTTGAATATATTG
CCGAAGCGGTCACTAAGGGGTTGATGGACgtacgtactatgaagcttagc
gcaagggatgacggactcagacatgataacaaacaatcatttaatcctat
tgtctattaaccccatttatccactaaaaaaagATCCAGCTGCAGACGAC
CATCCCGGTCATTTTCGGCCTCCTGACCGTGCTGAACAAAGACCAGGCGA
TTGTCCGTTCTAAGGGTGAGAACAATCACGGTATATCCTGGGGCAAGACT
GCCGTGGAAATGGCACTGCTGCGCACTGCCGCCATGGCCAAGGGGCCGAA
TAGGgtgagtttatgtcgagggtagggaaggaggagggggttgggagtgg
gaaagggacagatgaaaggactcattccaatttgatatccttcaaaacag
GCAACGCTTCCCTTGACAGAGAAACCGGGTGATACGCCTGATGAAAAGAA
GCAGGAGCGCCAGTTTGGGTTCTAA
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protein sequence of NO05G00960.1

>NO05G00960.1-protein ID=NO05G00960.1-protein|Name=NO05G00960.1|organism=Nannochloropsis oceanica|type=polypeptide|length=235bp
MLRFPSLVATTLLLLACVLRQATAFVMPRPALPTMSSNSRMLIAPLSMSK
DLVGFGEDLDGSACRIGIVKTRWNPEIVDSLYEGVRAALKETGVKDANIY
DTTVPGSFELPLAARFLAMSRQCDVVITIGCLIKGDTMHFEYIAEAVTKG
LMDIQLQTTIPVIFGLLTVLNKDQAIVRSKGENNHGISWGKTAVEMALLR
TAAMAKGPNRATLPLTEKPGDTPDEKKQERQFGF*
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Synonyms
Publications