NO03G04660, NO03G04660 (gene) Nannochloropsis oceanica

Overview
NameNO03G04660
Unique NameNO03G04660
Typegene
OrganismNannochloropsis oceanica (N. oceanica IMET1)
Sequence length1460
Alignment locationchr3:1333466..1334925 -

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Properties
Property NameValue
Description2fe-2s iron-sulfur cluster binding domain protein
Mutants
Expression

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
chr3genomechr3:1333466..1334925 -
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
PRJNA7699582024-08-13
GSE1786722023-12-15
PRJNA9336932023-10-26
PRJNA9434492023-07-19
PXD0160542021-01-14
PXD0166992021-01-08
GSE1499042020-10-10
NoIMET1_WT_HS2020-09-29
GSE1396152020-03-11
PRJNA2413822020-03-11
BLASTP analysis of N. oceanica IMET1 genes2019-07-11
InterPro analysis for N. oceanica IMET1 genes2019-07-11
GO annotation for IMET1v2 genes2019-07-10
PRJNA1821802019-04-25
Gene prediction for N. oceanica IMET12017-10-24
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0051536iron-sulfur cluster binding
GO:0009055electron transfer activity
GO:0005488binding
GO:0003824catalytic activity
Vocabulary: INTERPRO
TermDefinition
IPR0360102Fe-2S_ferredoxin-like_sf
IPR0010412Fe-2S_ferredoxin-type
Vocabulary: Biological Process
TermDefinition
GO:0006091generation of precursor metabolites and energy
Homology
BLAST of NO03G04660 vs. NCBI_GenBank
Match: EWM29895.1 (2fe-2s iron-sulfur cluster binding domain protein [Nannochloropsis gaditana])

HSP 1 Score: 301.2 bits (770), Expect = 2.100e-78
Identity = 148/183 (80.87%), Postives = 160/183 (87.43%), Query Frame = 0
Query:    1 MASTLLAKTMLRQAVVVGARGPAVAARAAG--VGSTGSS-RGLSWVKDVTVNVTFINHEGLRITVPGRVGQSLLDVAQMHDIELESGCGGGGAEQQKKHSERWTEDLFGRGPCCHFCHVKIPSPWIDFVEDKQPATDKENELLQEHWVEEASANSRLGCQVTLTKSLDGIQVFIPDGPPADCP 181
            MAS LLAK  LRQA+ VGA   A   R +G  + STGSS R L+ VKDVTVNVTFINHEGLRITVPGRVGQSLLDVAQMHDIELESGCGGGGA QQKKHSERWTEDLFGRGPCCHFCHVKIPSPWI+ + DKQPAT+KE+ELL+EHW EEA+ NSRLGCQV LTK+LDG+QVFIPDGPPADCP
Sbjct:    1 MASALLAKKALRQALAVGAHSQATVGRRSGAVINSTGSSRRTLASVKDVTVNVTFINHEGLRITVPGRVGQSLLDVAQMHDIELESGCGGGGAVQQKKHSERWTEDLFGRGPCCHFCHVKIPSPWINTIHDKQPATEKEHELLEEHWQEEATDNSRLGCQVMLTKNLDGMQVFIPDGPPADCP 183          
BLAST of NO03G04660 vs. NCBI_GenBank
Match: CBJ30843.1 (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 131.3 bits (329), Expect = 2.900e-27
Identity = 74/171 (43.27%), Postives = 100/171 (58.48%), Query Frame = 0
Query:   10 MLRQAVVVGARGPAVAARAAGVGSTGSS----RGLSWVKDVTVNVTFINHEGLRITVPGRVGQSLLDVAQMHDIELESGCGGGGAEQQKKHSERWTEDLFGRGPCCHFCHVKIPSPWIDFVEDKQPATDKENELLQEHWVEEASANSRLGCQVTLTKSLDGIQVFIPDGPP 177
            MLR A+  G     +  RAA  G+  S+    R LS +++  V VTF++ EG R TV    GQ++ +VA+ H+I L+  C G     Q ++SE WTEDLFG+GP C  CHV +     +F E  +P  D E  LL+      A  +SRLGCQ+ L++ LDGI VFIPDGPP
Sbjct:    1 MLRTALQTGV--GCLPRRAASGGAVSSAQAGRRALSSLQEALVKVTFVDAEGDRETVSALPGQNIFEVAEKHEIALDGPCRGKAGAVQSRNSESWTEDLFGQGPVCASCHVMLTK---EFTEKIEPPLDSEISLLERKGELYAPGSSRLGCQIKLSRDLDGITVFIPDGPP 166          
BLAST of NO03G04660 vs. NCBI_GenBank
Match: OQS06186.1 (hypothetical protein THRCLA_01755 [Thraustotheca clavata])

HSP 1 Score: 106.7 bits (265), Expect = 7.700e-20
Identity = 55/139 (39.57%), Postives = 81/139 (58.27%), Query Frame = 0
Query:   42 WVKDVTVNVTFINHEGLRITVPGRVGQSLLDVAQMH--DIELESGCGGGGAEQQKKHSERWTEDLFGRGPCCHFCHVKIPSPWIDFVEDKQPATDKENELLQEHWVEEASANSRLGCQVTLTKSLDGIQVFIPDGPPAD 179
            +V D  V+VT ++ EG R  V GRVGQ+L D  Q+   DI  +   GGGGA      +  +TE LFG GP     HV + + W + +    PA+D+E  +L++   E+ + NSRLG ++ LTK LDG+ V +P+ PP +
Sbjct:   26 FVHDRLVSVTLLDWEGSRHIVQGRVGQTLFDACQLSGVDIVKDDSLGGGGATYSAIRTPEFTESLFGEGPASPLSHVVVSNEWFNKL---PPASDRELRILEDVPEEDLTKNSRLGTEIILTKDLDGLVVAVPEAPPVE 161          
BLAST of NO03G04660 vs. NCBI_GenBank
Match: XP_008603877.1 (hypothetical protein SDRG_00189 [Saprolegnia diclina VS20] >EQC42454.1 hypothetical protein SDRG_00189 [Saprolegnia diclina VS20])

HSP 1 Score: 102.8 bits (255), Expect = 1.100e-18
Identity = 53/140 (37.86%), Postives = 82/140 (58.57%), Query Frame = 0
Query:   42 WVKDVTVNVTFINHEGLRITVPGRVGQSLLDVAQMH--DIELESGCGGGGAEQQKKHSERWTEDLFGRGPCCHFCHVKIPSPWIDFVEDKQPA-TDKENELLQEHWVEEASANSRLGCQVTLTKSLDGIQVFIPDGPPAD 179
            +V+D  V VT +++EG R  + GRVGQ+L +  Q+   DI  +   GGGG+      +  +TE LFG GP     HV + + W+     K PA T++E  +L++   E+ + NSRLG ++ LTK LDG+ V +P+ PP +
Sbjct:   26 FVRDHLVAVTLLDYEGSRHIIQGRVGQTLFEACQLSGLDIVKDDSLGGGGSAYSAVRTPEFTESLFGEGPSSPLSHVVVSNEWV----HKLPAPTEREARILEDVPEEDLTKNSRLGTEIILTKDLDGLVVAVPEAPPVE 161          
BLAST of NO03G04660 vs. NCBI_GenBank
Match: XP_012205775.1 (hypothetical protein SPRG_11382 [Saprolegnia parasitica CBS 223.65] >KDO23459.1 hypothetical protein SPRG_11382 [Saprolegnia parasitica CBS 223.65])

HSP 1 Score: 100.9 bits (250), Expect = 4.300e-18
Identity = 52/140 (37.14%), Postives = 81/140 (57.86%), Query Frame = 0
Query:   42 WVKDVTVNVTFINHEGLRITVPGRVGQSLLDVAQMH--DIELESGCGGGGAEQQKKHSERWTEDLFGRGPCCHFCHVKIPSPWIDFVEDKQPA-TDKENELLQEHWVEEASANSRLGCQVTLTKSLDGIQVFIPDGPPAD 179
            +V+D  V VT +++EG R  + GRVGQ+L +  Q+   DI  +   GGGGA      +  +TE LFG GP     HV + + W+     K P  +++E  +L++   E+ + NSRLG ++ LTK LDG+ V +P+ PP +
Sbjct:   26 FVRDHLVAVTLLDYEGSRHIIQGRVGQTLFEACQLSGLDIVKDDSLGGGGATYSAVRTPEFTESLFGEGPSSPLSHVVVSNEWV----HKLPVPSEREARILEDVPEEDLTKNSRLGTEIILTKDLDGLVVAVPEAPPVE 161          
BLAST of NO03G04660 vs. NCBI_GenBank
Match: OQR93588.1 (hypothetical protein ACHHYP_02409 [Achlya hypogyna])

HSP 1 Score: 98.6 bits (244), Expect = 2.100e-17
Identity = 60/159 (37.74%), Postives = 90/159 (56.60%), Query Frame = 0
Query:   24 VAARAAGVGS-TGSSRGLSWVKDVTVNVTFINHEGLRITVPGRVGQSLLDVAQMHDIEL--ESGCGGGGAEQQKKHSERWTEDLFGRGPCCHFCHVKIPSPWIDFVEDKQP-ATDKENELLQEHWVEEASANSRLGCQVTLTKSLDGIQVFIPDGPPAD 179
            VAA A   GS    S    +V+D  V+VT ++ EG R  V GRVGQ+L +  Q+  ++L  +   GGGGA      S  +TE+LFG GP     HV I + W+     K P  +++E  +L++   E+ + NSRLG ++ LTK L+G+ V +P+ PP +
Sbjct:    6 VAASARRFGSHAPKSVPEQFVRDHLVSVTLLDWEGSRHLVQGRVGQTLFEACQLAGLDLVKDDSLGGGGAMYSAVRSPEFTENLFGEGPSSPLSHVVISNEWV----HKLPLPSEREARILEDVPEEDLTKNSRLGTEIILTKDLEGLVVAVPEAPPIE 160          
BLAST of NO03G04660 vs. NCBI_GenBank
Match: XP_008870855.1 (hypothetical protein H310_07275 [Aphanomyces invadans] >ETW00720.1 hypothetical protein H310_07275 [Aphanomyces invadans])

HSP 1 Score: 97.4 bits (241), Expect = 4.700e-17
Identity = 52/139 (37.41%), Postives = 83/139 (59.71%), Query Frame = 0
Query:   42 WVKDVTVNVTFINHEGLRITVPGRVGQSLLDVAQMH--DIELESGCGGGGAEQQKKHSERWTEDLFGRGPCCHFCHVKIPSPWIDFVEDKQPATDKENELLQEHWVEEASANSRLGCQVTLTKSLDGIQVFIPDGPPAD 179
            +V+D  V+VT ++ EG R  V GRVGQSL + +++   DI  +   GGGGA      +  +TE+LFG GP     HV + + W+D +   QP T++E  +LQ+   E+ + NSRL  ++ L+K L+G+ V +P+  P +
Sbjct:   25 FVRDQIVSVTLLDWEGSRHIVQGRVGQSLFEASRLAGLDIIQDDSLGGGGASYSAVRTPEFTENLFGEGPSSFLSHVVVSNEWVDKL--PQP-TEREVRILQDVPEEDWTENSRLATEIILSKDLEGLVVAVPEAQPIE 160          
BLAST of NO03G04660 vs. NCBI_GenBank
Match: XP_009826239.1 (hypothetical protein H257_03721 [Aphanomyces astaci] >ETV84547.1 hypothetical protein H257_03721 [Aphanomyces astaci])

HSP 1 Score: 97.1 bits (240), Expect = 6.100e-17
Identity = 52/140 (37.14%), Postives = 82/140 (58.57%), Query Frame = 0
Query:   42 WVKDVTVNVTFINHEGLRITVPGRVGQSLLDVAQMH--DIELESGCGGGGAEQQKKHSERWTEDLFGRGPCCHFCHVKIPSPWIDFVEDKQP-ATDKENELLQEHWVEEASANSRLGCQVTLTKSLDGIQVFIPDGPPAD 179
            +V+D  V+VT ++ EG R  V GRVGQSL + +++   DI  +   GGGGA      +  +TE+LFG GP     HV + + W+    DK P  TD+E  +L++   ++ + NSRL  ++ LTK L+G+ V +P+  P +
Sbjct:   25 FVRDQIVSVTLLDWEGSRHVVQGRVGQSLFEASRLSGLDIIQDDTLGGGGAAYSAIRTPEFTENLFGEGPASFLSHVVVSNEWV----DKLPLPTDREVRILEDVPEDDWTKNSRLATEIILTKDLEGLVVAVPEAQPIE 160          
BLAST of NO03G04660 vs. NCBI_GenBank
Match: XP_012893672.1 (uncharacterized protein [Blastocystis hominis] >CBK19624.2 unnamed protein product [Blastocystis hominis])

HSP 1 Score: 95.9 bits (237), Expect = 1.400e-16
Identity = 53/142 (37.32%), Postives = 82/142 (57.75%), Query Frame = 0
Query:   38 RGLSW---VKDVTVNVTFINHEGLRITVPGRVGQSLLDVAQMHDIELESGCGGGGAEQQKKHSERWTEDLFGRGPCCHFCHVKIPSPWIDFVEDKQPATDKENELLQEHWVEEASANSRLGCQVTLTKSLDGIQVFIPDGPP 177
            R +SW   V + T+++TFI  +G   TV  R+G ++LD A+ + + +   C G G  +  K +E WTE+ FG GP C FCHV +PS  +  V +K   T +E E +++  + + +  SRL CQV +TK ++G   FIP   P
Sbjct:   21 RSISWTSAVAENTIDLTFITPKGESKTVKARIGDTVLDTAEAYGLPICGDCHGAGLPRAVKRTENWTEETFGEGPSCAFCHV-VPSLELSKVLEK--PTAQELEFIEKIPIGK-TPRSRLACQVPVTKEMNGGVFFIPHHVP 158          
BLAST of NO03G04660 vs. NCBI_GenBank
Match: AFL03354.1 (putative 2Fe-2S ferredoxin [Blastocystis sp. ATCC 50177/Nand II] >OAO17976.1 Ferredoxin [Blastocystis sp. ATCC 50177/Nand II])

HSP 1 Score: 95.9 bits (237), Expect = 1.400e-16
Identity = 52/144 (36.11%), Postives = 75/144 (52.08%), Query Frame = 0
Query:   36 SSRGLSW---VKDVTVNVTFINHEGLRITVPGRVGQSLLDVAQMHDIELESGCGGGGAEQQKKHSERWTEDLFGRGPCCHFCHVKIPSPWIDFVEDKQPATDKENELLQEHWVEEASANSRLGCQVTLTKSLDGIQVFIPDGPP 177
            ++R +SW     D T+++TFI  EG    V  R+G +LL+ A+ + + L   C G G  +  K +E WTE+ FG GP C FCHV +     + V    P    E E   E      +  SRL CQ+++TK ++G  VFIP   P
Sbjct:   19 AARFISWTGKAADNTIDITFITPEGESKPVKARIGDTLLETAEAYKLPLCGDCHGNGLPRAVKRTEGWTEETFGEGPSCSFCHVVLSRELSEVV----PKPCAEEETFIEKIPIGKTERSRLACQISVTKEMNGGVVFIPHHVP 158          
The following BLAST results are available for this feature:
BLAST of NO03G04660 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes)
Total hits: 20
Match NameE-valueIdentityDescription
EWM29895.12.100e-7880.872fe-2s iron-sulfur cluster binding domain protein ... [more]
CBJ30843.12.900e-2743.27conserved unknown protein [Ectocarpus siliculosus][more]
OQS06186.17.700e-2039.57hypothetical protein THRCLA_01755 [Thraustotheca c... [more]
XP_008603877.11.100e-1837.86hypothetical protein SDRG_00189 [Saprolegnia dicli... [more]
XP_012205775.14.300e-1837.14hypothetical protein SPRG_11382 [Saprolegnia paras... [more]
OQR93588.12.100e-1737.74hypothetical protein ACHHYP_02409 [Achlya hypogyna... [more]
XP_008870855.14.700e-1737.41hypothetical protein H310_07275 [Aphanomyces invad... [more]
XP_009826239.16.100e-1737.14hypothetical protein H257_03721 [Aphanomyces astac... [more]
XP_012893672.11.400e-1637.32uncharacterized protein [Blastocystis hominis] >CB... [more]
AFL03354.11.400e-1636.11putative 2Fe-2S ferredoxin [Blastocystis sp. ATCC ... [more]

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Relationships

This gene is member of the following syntenic_region feature(s):

Feature NameUnique NameSpeciesType
nonsL069nonsL069Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
nonsL066nonsL066Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ncniR000ncniR000Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ngnoR054ngnoR054Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ngnoR048ngnoR048Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region


This gene is orthologous to the following gene feature(s):

Feature NameUnique NameSpeciesType
NSK005418NSK005418Nannochloropsis salina (N. salina CCMP1776)gene


The following polypeptide feature(s) derives from this gene:

Feature NameUnique NameSpeciesType
NO03G04660.1NO03G04660.1-proteinNannochloropsis oceanica (N. oceanica IMET1)polypeptide


The following gene feature(s) are orthologous to this gene:

Feature NameUnique NameSpeciesType
Naga_100035g29gene734Nannochloropsis gaditana (N. gaditana B-31)gene


The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
NO03G04660.1NO03G04660.1Nannochloropsis oceanica (N. oceanica IMET1)mRNA


Sequences
The following sequences are available for this feature:

gene sequence

>NO03G04660 ID=NO03G04660|Name=NO03G04660|organism=Nannochloropsis oceanica|type=gene|length=1460bp
TCGGCTTAACAGTCAGAACTCGCATTGCAAGCTGCATGATGATCCTTGCC
CAACGTTTGGGGTCTTGCTTGGTGTATGCACAAGCGTCCGCCCACTACGT
CGGCTGTCTCAATCATCTTGTTAACATGTGGGTCTGTTGCTCAAGATGGT
AGTGGCCGTCAGAGCCGGCGGCGCACTGCATAGAGGCAAATGAACAAGAC
TTTCTCTGTCTCTCTATGCTTGATGATGTTGTCTTGTCCTCAGCCCCGGC
TCCCATACAAAACATCCTGCCGCATACACATAGGCAGCCATGGCATCGAC
GCTTCTGGCGAAGACGATGCTCCGCCAGGCCGTGGTAGTGGGAGCCCGCG
GGCCAGCAGTTGCAGCGAGAGCGGCCGGCGTAGGGAGCACCGGGAGCAGC
AGAGGTCTTTCTTGGGTCAAGGACGTGACAGTGAACGTCACCTTTATCAA
CCATGAAgtaagtatagagtgtgtgtatgtgttgaatgcgacaaaatgta
gggataatatggaccgttggcatttaaaaggcctcaatcagccactaatg
ccttcccctatcccctcgcacctctcgcacacacatatcagGGTCTGAGG
ATAACAGTGCCGGGCCGAGTGGGACAAAGCTTGCTGGACGTGGCGCAGAT
GCACGACATCGAGTTGGAGTCAGGATGCGGGGGTGGTGGGGCCGAGCAGC
AGAAAAAGCATTCGGAACGGTGGACGGAGGACTTGTTTGGACGAGgtgag
tgagcgtgggaggaaagcgagaggggaatgaggacggcaattctatggca
acgactgtcgatttagtggagcaggaaacaggtataagtagcgttaccac
ctgagtgtgaatggtttggtagcgtcgctttgcgacaccgttaatgccag
ggcccaaattcttctctagagtagttgctcacttcctctccctccttccc
cctcattcatcctctacagGCCCCTGCTGCCACTTCTGCCACGTTAAGAT
TCCTTCCCCCTGGATCGACTTTGTGGAGGATAAACAGCCGGCGACGGATA
AGGAGAATGAGCTGCTACAGGAGCATTGGGTGGAGGAAGCCTCGGCAAAC
TCTCGATTGGGGTGTCAAGTGACGTTGACCAAATCCTTGGACGGAATTCA
GGTATTTATCCCGGATGGACCGCCGGCAGATTGCCCGTAGACTCATTTGA
CCGATTGATATGGATGGATGGACGGATTGAGAGGAGGGAGGGAAGGAGGG
AGGTTTGTCGCGGCGTTCAGGAACGAGGTGTGGGCAGGGGCTTCAGAGCC
CTAGGGTAGTTAGTACAAGCGACAAGCGAGCTAGGAAGTGCAGAGGCCTT
TTTTATACGAGTAGGGGAAGTGGAGTGGAAACAGTGAGGATGACGAATAG
AGGAATACGGGGAAAGAACACCGTGCACAAATGATGAAATCAGTTGAGAA
CAGAGGGGAA
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protein sequence of NO03G04660.1

>NO03G04660.1-protein ID=NO03G04660.1-protein|Name=NO03G04660.1|organism=Nannochloropsis oceanica|type=polypeptide|length=181bp
MASTLLAKTMLRQAVVVGARGPAVAARAAGVGSTGSSRGLSWVKDVTVNV
TFINHEGLRITVPGRVGQSLLDVAQMHDIELESGCGGGGAEQQKKHSERW
TEDLFGRGPCCHFCHVKIPSPWIDFVEDKQPATDKENELLQEHWVEEASA
NSRLGCQVTLTKSLDGIQVFIPDGPPADCP*
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Synonyms
Publications