NO03G00060, NO03G00060 (gene) Nannochloropsis oceanica

Overview
NameNO03G00060
Unique NameNO03G00060
Typegene
OrganismNannochloropsis oceanica (N. oceanica IMET1)
Sequence length1235
Alignment locationchr3:16312..17546 -

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Properties
Property NameValue
DescriptionUbiquitin-conjugating enzyme e2
Mutants
Expression

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
chr3genomechr3:16312..17546 -
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
PRJNA7699582024-08-13
GSE1786722023-12-15
PRJNA9336932023-10-26
PRJNA9434492023-07-19
PXD0160542021-01-14
GSE1499042020-10-10
NoIMET1_WT_HS2020-09-29
GSE1396152020-03-11
PRJNA2413822020-03-11
BLASTP analysis of N. oceanica IMET1 genes2019-07-11
InterPro analysis for N. oceanica IMET1 genes2019-07-11
GO annotation for IMET1v2 genes2019-07-10
PRJNA1821802019-04-25
Gene prediction for N. oceanica IMET12017-10-24
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR016135UBQ-conjugating_enzyme/RWD
IPR000608UBQ-conjugat_E2
Vocabulary: Molecular Function
TermDefinition
GO:0016740transferase activity
GO:0000166nucleotide binding
Homology
BLAST of NO03G00060 vs. NCBI_GenBank
Match: EWM29983.1 (ubiquitin-conjugating enzyme e2 [Nannochloropsis gaditana])

HSP 1 Score: 313.2 bits (801), Expect = 5.700e-82
Identity = 149/150 (99.33%), Postives = 150/150 (100.00%), Query Frame = 0
Query:    1 MSTAARRRLMRDLKRLQSDPPQGVQGAPLDNNIMTWQAVIFGPDDTPWEGGTFKLLLEFTEDYPNKAPAVRFVTKMFHPNIYNDGQICLDILQNQWSPIYDVSAILTSIQSLLCDPNPASPANSEASRLYQDNRREYNRRVKEIVEQSWI 151
            MSTAARRRLMRDLKRLQSDPPQGVQGAPLDNNIMTWQAVIFGPDDTPWEGGTFKLLLEFTEDYPNKAPAVRFVTKMFHPNIYNDGQICLDILQNQWSPIYDVSAILTSIQSLLCDPNPASPANSEASRLYQDNRREYNRRVKE+VEQSWI
Sbjct:    1 MSTAARRRLMRDLKRLQSDPPQGVQGAPLDNNIMTWQAVIFGPDDTPWEGGTFKLLLEFTEDYPNKAPAVRFVTKMFHPNIYNDGQICLDILQNQWSPIYDVSAILTSIQSLLCDPNPASPANSEASRLYQDNRREYNRRVKEVVEQSWI 150          
BLAST of NO03G00060 vs. NCBI_GenBank
Match: XP_009844417.1 (ubiquitin-conjugating enzyme E2 2 [Aphanomyces astaci] >ETV66088.1 ubiquitin-conjugating enzyme E2 2 [Aphanomyces astaci])

HSP 1 Score: 290.0 bits (741), Expect = 5.100e-75
Identity = 133/151 (88.08%), Postives = 145/151 (96.03%), Query Frame = 0
Query:    1 MSTAARRRLMRDLKRLQSDPPQGVQGAPLDNNIMTWQAVIFGPDDTPWEGGTFKLLLEFTEDYPNKAPAVRFVTKMFHPNIYNDGQICLDILQNQWSPIYDVSAILTSIQSLLCDPNPASPANSEASRLYQDNRREYNRRVKEIVEQSWIA 152
            MSTAARRRLMRD ++LQ+DPP GV GAPLDNNIM WQAVIFGPDDTPWEGGTF LLLEF+EDYPNKAP+V+FVTKMFHPNIYNDGQICLDILQNQWSPIYD+SAILTSIQSLLCDPNP SPANSEA+RL+Q+NRREYNRRV+EIVEQSW+A
Sbjct:    1 MSTAARRRLMRDFRKLQNDPPSGVSGAPLDNNIMVWQAVIFGPDDTPWEGGTFNLLLEFSEDYPNKAPSVKFVTKMFHPNIYNDGQICLDILQNQWSPIYDISAILTSIQSLLCDPNPNSPANSEAARLFQENRREYNRRVREIVEQSWVA 151          
BLAST of NO03G00060 vs. NCBI_GenBank
Match: XP_008879285.1 (ubiquitin-conjugating enzyme E2 2 [Aphanomyces invadans] >ETV92123.1 ubiquitin-conjugating enzyme E2 2 [Aphanomyces invadans])

HSP 1 Score: 290.0 bits (741), Expect = 5.100e-75
Identity = 133/151 (88.08%), Postives = 145/151 (96.03%), Query Frame = 0
Query:    1 MSTAARRRLMRDLKRLQSDPPQGVQGAPLDNNIMTWQAVIFGPDDTPWEGGTFKLLLEFTEDYPNKAPAVRFVTKMFHPNIYNDGQICLDILQNQWSPIYDVSAILTSIQSLLCDPNPASPANSEASRLYQDNRREYNRRVKEIVEQSWIA 152
            MSTAARRRLMRD ++LQ+DPP GV GAPLDNNIM WQAVIFGPDDTPWEGGTF LLLEF+EDYPNKAP+V+FVTKMFHPNIYNDGQICLDILQNQWSPIYD+SAILTSIQSLLCDPNP SPANSEA+RL+Q+NRREYNRRV+EIVEQSW+A
Sbjct:    1 MSTAARRRLMRDFRKLQNDPPSGVSGAPLDNNIMVWQAVIFGPDDTPWEGGTFNLLLEFSEDYPNKAPSVKFVTKMFHPNIYNDGQICLDILQNQWSPIYDISAILTSIQSLLCDPNPNSPANSEAARLFQENRREYNRRVREIVEQSWVA 151          
BLAST of NO03G00060 vs. NCBI_GenBank
Match: XP_002179401.1 (predicted protein [Phaeodactylum tricornutum CCAP 1055/1] >EEC49224.1 predicted protein [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 288.9 bits (738), Expect = 1.100e-74
Identity = 133/150 (88.67%), Postives = 143/150 (95.33%), Query Frame = 0
Query:    1 MSTAARRRLMRDLKRLQSDPPQGVQGAPLDNNIMTWQAVIFGPDDTPWEGGTFKLLLEFTEDYPNKAPAVRFVTKMFHPNIYNDGQICLDILQNQWSPIYDVSAILTSIQSLLCDPNPASPANSEASRLYQDNRREYNRRVKEIVEQSWI 151
            MST+ARRRL+RD KRLQ+DPP GV GAP+D NIM WQAVIFGPDDTPWEGGTFKL+LEFTEDYPNKAP VRF+TKMFHPNIYNDGQICLDILQNQWSPIYD+SAILTSIQSLLCDPNPASPANSEASRLY +NRREYNRRV+EIVEQSW+
Sbjct:    1 MSTSARRRLLRDFKRLQNDPPTGVTGAPMDTNIMMWQAVIFGPDDTPWEGGTFKLVLEFTEDYPNKAPQVRFLTKMFHPNIYNDGQICLDILQNQWSPIYDISAILTSIQSLLCDPNPASPANSEASRLYNENRREYNRRVREIVEQSWV 150          
BLAST of NO03G00060 vs. NCBI_GenBank
Match: XP_002293014.1 (ubiquitin-conjugating enzyme 1 [Thalassiosira pseudonana CCMP1335] >EED89475.1 ubiquitin-conjugating enzyme 1 [Thalassiosira pseudonana CCMP1335])

HSP 1 Score: 288.9 bits (738), Expect = 1.100e-74
Identity = 132/150 (88.00%), Postives = 144/150 (96.00%), Query Frame = 0
Query:    1 MSTAARRRLMRDLKRLQSDPPQGVQGAPLDNNIMTWQAVIFGPDDTPWEGGTFKLLLEFTEDYPNKAPAVRFVTKMFHPNIYNDGQICLDILQNQWSPIYDVSAILTSIQSLLCDPNPASPANSEASRLYQDNRREYNRRVKEIVEQSWI 151
            MST+ARRRL+RD +RLQ+DPP GV GAP+D NIM WQAVIFGPDDTPWEGGTFKL+LEFTEDYPNKAP+VRF+TKMFHPNIYNDGQICLDILQNQWSPIYD+SAILTSIQSLLCDPNPASPANSEASRLY +NRREYNRRV+EIVEQSW+
Sbjct:    1 MSTSARRRLLRDFRRLQNDPPSGVTGAPMDTNIMLWQAVIFGPDDTPWEGGTFKLVLEFTEDYPNKAPSVRFLTKMFHPNIYNDGQICLDILQNQWSPIYDISAILTSIQSLLCDPNPASPANSEASRLYNENRREYNRRVREIVEQSWV 150          
BLAST of NO03G00060 vs. NCBI_GenBank
Match: XP_008618216.1 (ubiquitin-conjugating enzyme E2 2 [Saprolegnia diclina VS20] >XP_012197338.1 ubiquitin-conjugating enzyme E2 2 [Saprolegnia parasitica CBS 223.65] >EQC28346.1 ubiquitin-conjugating enzyme E2 2 [Saprolegnia diclina VS20] >KDO32154.1 ubiquitin-conjugating enzyme E2 2 [Saprolegnia parasitica CBS 223.65] >OQR85920.1 ubiquitin-conjugating enzyme E2 [Achlya hypogyna])

HSP 1 Score: 288.5 bits (737), Expect = 1.500e-74
Identity = 133/151 (88.08%), Postives = 144/151 (95.36%), Query Frame = 0
Query:    1 MSTAARRRLMRDLKRLQSDPPQGVQGAPLDNNIMTWQAVIFGPDDTPWEGGTFKLLLEFTEDYPNKAPAVRFVTKMFHPNIYNDGQICLDILQNQWSPIYDVSAILTSIQSLLCDPNPASPANSEASRLYQDNRREYNRRVKEIVEQSWIA 152
            MSTAARRRLMRD ++LQ+DPP GV GAPLDNNIM WQAVIFGPDDTPWEGGTF LLLEF+EDYPNKAP+V+FVTKMFHPNIYNDGQICLDILQNQWSPIYD+SAILTSIQSLLCDPNP SPANSEA+RL+Q+NRREYNRRV+EIVEQSW A
Sbjct:    1 MSTAARRRLMRDFRKLQNDPPSGVSGAPLDNNIMVWQAVIFGPDDTPWEGGTFNLLLEFSEDYPNKAPSVKFVTKMFHPNIYNDGQICLDILQNQWSPIYDISAILTSIQSLLCDPNPNSPANSEAARLFQENRREYNRRVREIVEQSWAA 151          
BLAST of NO03G00060 vs. NCBI_GenBank
Match: OEU22022.1 (ubiquitin conjugating protein [Fragilariopsis cylindrus CCMP1102])

HSP 1 Score: 288.5 bits (737), Expect = 1.500e-74
Identity = 134/152 (88.16%), Postives = 143/152 (94.08%), Query Frame = 0
Query:    1 MSTAARRRLMRDLKRLQSDPPQGVQGAPLDNNIMTWQAVIFGPDDTPWEGGTFKLLLEFTEDYPNKAPAVRFVTKMFHPNIYNDGQICLDILQNQWSPIYDVSAILTSIQSLLCDPNPASPANSEASRLYQDNRREYNRRVKEIVEQSWIAD 153
            MST+ARRRL+RD KRLQ+DPP GV GAP+D NIM WQAVIFGPDDTPWEGGTFKL+LEFTEDYPNKAP VRF+TKMFHPNIYNDGQICLDILQNQWSPIYD+SAILTSIQSLLCDPNPASPANSEASRLY +NRREYNRRV+EIVEQSW  D
Sbjct:    1 MSTSARRRLLRDFKRLQNDPPSGVTGAPMDTNIMLWQAVIFGPDDTPWEGGTFKLVLEFTEDYPNKAPQVRFLTKMFHPNIYNDGQICLDILQNQWSPIYDISAILTSIQSLLCDPNPASPANSEASRLYNENRREYNRRVREIVEQSWQDD 152          
BLAST of NO03G00060 vs. NCBI_GenBank
Match: OQR93556.1 (hypothetical protein THRCLA_08419 [Thraustotheca clavata])

HSP 1 Score: 286.6 bits (732), Expect = 5.700e-74
Identity = 132/151 (87.42%), Postives = 143/151 (94.70%), Query Frame = 0
Query:    1 MSTAARRRLMRDLKRLQSDPPQGVQGAPLDNNIMTWQAVIFGPDDTPWEGGTFKLLLEFTEDYPNKAPAVRFVTKMFHPNIYNDGQICLDILQNQWSPIYDVSAILTSIQSLLCDPNPASPANSEASRLYQDNRREYNRRVKEIVEQSWIA 152
            MSTAARRRLMRD ++LQ+DPP GV GAPLDNNIM WQAVIFGPDDTPWEGGTF L+LEF+EDYPNKAP V+FVTKMFHPNIYNDGQICLDILQNQWSPIYD+SAILTSIQSLLCDPNP SPANSEA+RL+Q+NRREYNRRV+EIVEQSW A
Sbjct:  638 MSTAARRRLMRDFRKLQNDPPSGVSGAPLDNNIMVWQAVIFGPDDTPWEGGTFNLILEFSEDYPNKAPHVKFVTKMFHPNIYNDGQICLDILQNQWSPIYDISAILTSIQSLLCDPNPNSPANSEAARLFQENRREYNRRVREIVEQSWAA 788          
BLAST of NO03G00060 vs. NCBI_GenBank
Match: GAY06340.1 (ubiquitinconjugating enzyme E2 putative [Pythium insidiosum] >GAY04819.1 ubiquitinconjugating enzyme E2 putative [Pythium insidiosum] >GAX99202.1 ubiquitinconjugating enzyme E2 putative [Pythium insidiosum])

HSP 1 Score: 285.0 bits (728), Expect = 1.700e-73
Identity = 129/151 (85.43%), Postives = 143/151 (94.70%), Query Frame = 0
Query:    1 MSTAARRRLMRDLKRLQSDPPQGVQGAPLDNNIMTWQAVIFGPDDTPWEGGTFKLLLEFTEDYPNKAPAVRFVTKMFHPNIYNDGQICLDILQNQWSPIYDVSAILTSIQSLLCDPNPASPANSEASRLYQDNRREYNRRVKEIVEQSWIA 152
            MSTAARRRLMRD ++LQ+DPP GV GAP+DNNIM WQAVIFGPDDTPWEGGTF L LEF+EDYPNKAP V+F+TKMFHPNIYNDGQICLDILQNQWSPIYD+SAILTSIQSLLCDPNP SPANSEA+RL+Q+NRREYNRRV+EIVEQSW++
Sbjct:    1 MSTAARRRLMRDFRKLQNDPPSGVSGAPMDNNIMLWQAVIFGPDDTPWEGGTFNLTLEFSEDYPNKAPVVKFITKMFHPNIYNDGQICLDILQNQWSPIYDISAILTSIQSLLCDPNPNSPANSEAARLFQENRREYNRRVREIVEQSWVS 151          
BLAST of NO03G00060 vs. NCBI_GenBank
Match: CCA22385.1 (ubiquitinconjugating enzyme E2 putative [Albugo laibachii Nc14])

HSP 1 Score: 284.6 bits (727), Expect = 2.200e-73
Identity = 129/151 (85.43%), Postives = 143/151 (94.70%), Query Frame = 0
Query:    1 MSTAARRRLMRDLKRLQSDPPQGVQGAPLDNNIMTWQAVIFGPDDTPWEGGTFKLLLEFTEDYPNKAPAVRFVTKMFHPNIYNDGQICLDILQNQWSPIYDVSAILTSIQSLLCDPNPASPANSEASRLYQDNRREYNRRVKEIVEQSWIA 152
            MSTAARRRLMRD ++LQ+DPP G+ GAP+DNNIM WQAVIFGPDDTPWEGGTF L LEF+EDYPNKAP V+F+TKMFHPNIYNDGQICLDILQNQWSPIYD+SAILTSIQSLLCDPNP SPANSEA+RL+Q+NRREYNRRV+EIVEQSW+A
Sbjct:   19 MSTAARRRLMRDFRKLQNDPPCGISGAPMDNNIMMWQAVIFGPDDTPWEGGTFNLTLEFSEDYPNKAPTVKFITKMFHPNIYNDGQICLDILQNQWSPIYDISAILTSIQSLLCDPNPNSPANSEAARLFQENRREYNRRVREIVEQSWVA 169          
The following BLAST results are available for this feature:
BLAST of NO03G00060 vs. NCBI_GenBank
Analysis Date: 2019-07-11 (BLASTP analysis of N. oceanica IMET1 genes)
Total hits: 20
Match NameE-valueIdentityDescription
EWM29983.15.700e-8299.33ubiquitin-conjugating enzyme e2 [Nannochloropsis g... [more]
XP_009844417.15.100e-7588.08ubiquitin-conjugating enzyme E2 2 [Aphanomyces ast... [more]
XP_008879285.15.100e-7588.08ubiquitin-conjugating enzyme E2 2 [Aphanomyces inv... [more]
XP_002179401.11.100e-7488.67predicted protein [Phaeodactylum tricornutum CCAP ... [more]
XP_002293014.11.100e-7488.00ubiquitin-conjugating enzyme 1 [Thalassiosira pseu... [more]
XP_008618216.11.500e-7488.08ubiquitin-conjugating enzyme E2 2 [Saprolegnia dic... [more]
OEU22022.11.500e-7488.16ubiquitin conjugating protein [Fragilariopsis cyli... [more]
OQR93556.15.700e-7487.42hypothetical protein THRCLA_08419 [Thraustotheca c... [more]
GAY06340.11.700e-7385.43ubiquitinconjugating enzyme E2 putative [Pythium i... [more]
CCA22385.12.200e-7385.43ubiquitinconjugating enzyme E2 putative [Albugo la... [more]

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Relationships

This gene is member of the following syntenic_region feature(s):

Feature NameUnique NameSpeciesType
nonsL071nonsL071Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ncniR012ncniR012Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region
ngnoR055ngnoR055Nannochloropsis oceanica (N. oceanica IMET1)syntenic_region


This gene is orthologous to the following gene feature(s):

Feature NameUnique NameSpeciesType
NSK005911NSK005911Nannochloropsis salina (N. salina CCMP1776)gene


The following polypeptide feature(s) derives from this gene:

Feature NameUnique NameSpeciesType
NO03G00060.1NO03G00060.1-proteinNannochloropsis oceanica (N. oceanica IMET1)polypeptide


The following gene feature(s) are orthologous to this gene:

Feature NameUnique NameSpeciesType
jgi.p|Nanoce1779_2|634247gene_10431Nannochloropsis oceanica (N. oceanica CCMP1779)gene
Naga_100276g2gene661Nannochloropsis gaditana (N. gaditana B-31)gene


The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
NO03G00060.1NO03G00060.1Nannochloropsis oceanica (N. oceanica IMET1)mRNA


Sequences
The following sequences are available for this feature:

gene sequence

>NO03G00060 ID=NO03G00060|Name=NO03G00060|organism=Nannochloropsis oceanica|type=gene|length=1235bp
ATGTCCACCGCTGCCCGCCGCCGTCTGATGCGCGACTTGAAGCGGCTACA
GAGCGACCCTCCACAAGGCGTTCAGGGCGCACCCCTCGACAACAATgtac
gttcgatcgacgcgatggaatataagaaatagctgttgctgcgctgtcca
cccgagacagtcccctaaagccctccttttattccttcctgcttccctct
ctcccagATCATGACCTGGCAGGCGGTCATCTTCGGACCTGACGACACGC
CCTGGGAAGGCGGCACTTTTAAGCTGCTCCTTGAGTTCACAGAGGACTAC
CCCAACAAGgtaggcagggagggagggaggagttggtatgcaggcagcga
gtagctcaagcattttatcggtgcattgtgacagggtgcgataagagcca
gacagccatcatttcatacatctggccctgtcataatccccttcctccct
ttctctcttctttctttctcccgccatagGCTCCGGCGGTGCGATTTGTG
ACGAAGATGTTCCACCCCAACATTTACAACGACGGCCAGATCTGTCTCGg
tacgttggtagggagaaggaaaaaaagggaaagaaaaagggacacgagga
agtgaggaggagaagagcgacaaattgtgtaaatgtaaggggtgtagaag
ctgaggtgccactttatgcgcccctccctcattgccctcctccttgtcaa
ccccctcctttccctgactagACATCCTGCAAAACCAGTGGAGCCCCATC
TATGACGTCTCGGCCATCCTCACCTCCATCCAGTCCTTGCTGTGCGACCC
CAACCCTGCGTCGCCCGCCAACAGTGAGGCCTCGCGTCTGTACCAGgcac
gtgatagcgtagcctcgccctccttccttccctacttcgctccctcccgt
cttatttttgcagctttagttcgttggtttagcgaagcgaatatttttgg
ttgccgcagagaggagtgaggaaaagtccttttatttcaaattaatacct
gtaccatcaacccttcactcgtcctccgtccctctccccctctctctatc
cgtcccccccttagGACAATCGGCGAGAGTACAATCGCCGCGTGAAGGAG
ATTGTCGAACAAAGCTGGATCGCGGATGGCGACGACGACGAGGATGAGGA
GGAAGAGGAGGAGGAAGAGGAGGAGGAGGTAGAGGGCGAGGAAGGGGGTA
AGGGAGAGTCGGCGGCGGAAGGTGAGCATGCATGA
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protein sequence of NO03G00060.1

>NO03G00060.1-protein ID=NO03G00060.1-protein|Name=NO03G00060.1|organism=Nannochloropsis oceanica|type=polypeptide|length=188bp
MSTAARRRLMRDLKRLQSDPPQGVQGAPLDNNIMTWQAVIFGPDDTPWEG
GTFKLLLEFTEDYPNKAPAVRFVTKMFHPNIYNDGQICLDILQNQWSPIY
DVSAILTSIQSLLCDPNPASPANSEASRLYQDNRREYNRRVKEIVEQSWI
ADGDDDEDEEEEEEEEEEEVEGEEGGKGESAAEGEHA*
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Synonyms
Publications