EWM20757.1, cds9914 (CDS) Nannochloropsis gaditana

Overview
NameEWM20757.1
Unique Namecds9914
TypeCDS
OrganismNannochloropsis gaditana (N. gaditana B-31)
Alignment locationKK037258.1:61852..62835 -

Link to JBrowse

Properties
Property NameValue
Protein idEWM20757.1
Producthypothetical protein
Orig transcript idgnl|cribi|Naga_100518g2.7414.mrna
GbkeyCDS
Mutants
Expression
No biomaterial libraries express this feature.
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
KK037258.1supercontigKK037258.1:61852..62835 -
Analyses
This CDS is derived from or has results from the following analyses
Analysis NameDate Performed
GO annotation for N. gaditana B312020-04-08
BLAST analysis for N. gaditana B-312020-04-07
InterPro analysis for N. gaditana B-312020-04-06
Gene prediction for N. gaditana B-312014-02-18
Annotated Terms
The following terms have been associated with this CDS:
Vocabulary: Molecular Function
TermDefinition
GO:0016740transferase activity
GO:0005515protein binding
Vocabulary: INTERPRO
TermDefinition
IPR004045Glutathione_S-Trfase_N
IPR012336Thioredoxin-like_fold
Homology
BLAST of EWM20757.1 vs. NCBI_GenBank
Match: gi|585100630|gb|EWM20757.1| (hypothetical protein Naga_100518g2 [Nannochloropsis gaditana])

HSP 1 Score: 668.307 bits (1723), Expect = 0.000e+0
Identity = 327/327 (100.00%), Postives = 327/327 (100.00%), Query Frame = 0
Query:    1 MDHVLRTVGWFVRTPEVPKDGAKLCTIALSHYCEKARWALDLSPLRDTYIEDAHAPVFHYRPTVALTKDRKKTGLPVLVLPGVDPEGPPTVVQDSAEILQHLASLYPKELGHMYPPGDMGDKVRALERKLGEQLGPATRKIVYWEMWQGRQEGIDTCNALLERHYPTAEARMWRLLEAKILPVMLKAMKIDETSTLAALEESREIFREVSALLDSEEGGKGGGAPAGPRRYLLGANRMTAADLTFAALAYPLINPPAFASLGLSGAEASPWAKRLAVITKEFRATPAGQHVLRLYGEERFPETSMAGKEPTGQLCLRTSNRDKWPWQ 327
            MDHVLRTVGWFVRTPEVPKDGAKLCTIALSHYCEKARWALDLSPLRDTYIEDAHAPVFHYRPTVALTKDRKKTGLPVLVLPGVDPEGPPTVVQDSAEILQHLASLYPKELGHMYPPGDMGDKVRALERKLGEQLGPATRKIVYWEMWQGRQEGIDTCNALLERHYPTAEARMWRLLEAKILPVMLKAMKIDETSTLAALEESREIFREVSALLDSEEGGKGGGAPAGPRRYLLGANRMTAADLTFAALAYPLINPPAFASLGLSGAEASPWAKRLAVITKEFRATPAGQHVLRLYGEERFPETSMAGKEPTGQLCLRTSNRDKWPWQ
Sbjct:    1 MDHVLRTVGWFVRTPEVPKDGAKLCTIALSHYCEKARWALDLSPLRDTYIEDAHAPVFHYRPTVALTKDRKKTGLPVLVLPGVDPEGPPTVVQDSAEILQHLASLYPKELGHMYPPGDMGDKVRALERKLGEQLGPATRKIVYWEMWQGRQEGIDTCNALLERHYPTAEARMWRLLEAKILPVMLKAMKIDETSTLAALEESREIFREVSALLDSEEGGKGGGAPAGPRRYLLGANRMTAADLTFAALAYPLINPPAFASLGLSGAEASPWAKRLAVITKEFRATPAGQHVLRLYGEERFPETSMAGKEPTGQLCLRTSNRDKWPWQ 327          
BLAST of EWM20757.1 vs. NCBI_GenBank
Match: gi|553183975|ref|XP_005854005.1| (glutathione s-transferase [Nannochloropsis gaditana CCMP526] >gi|553192152|ref|XP_005855522.1| glutathione s-transferase [Nannochloropsis gaditana CCMP526] >gi|422293534|gb|EKU20834.1| glutathione s-transferase [Nannochloropsis gaditana CCMP526] >gi|422295055|gb|EKU22354.1| glutathione s-transferase [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 263.462 bits (672), Expect = 7.580e-86
Identity = 126/127 (99.21%), Postives = 127/127 (100.00%), Query Frame = 0
Query:    1 MDHVLRTVGWFVRTPEVPKDGAKLCTIALSHYCEKARWALDLSPLRDTYIEDAHAPVFHYRPTVALTKDRKKTGLPVLVLPGVDPEGPPTVVQDSAEILQHLASLYPKELGHMYPPGDMGDKVRALE 127
            MDHVLRTVGWFVRTPEVPKDGAKLCTIALSHYCEKARWALDLSPLRDTYIEDAHAPVFHYRPTVALTKDRKKTGLPVLVLPGVDPEGPPTVVQDSAEILQHLASLYPKELGH+YPPGDMGDKVRALE
Sbjct:    1 MDHVLRTVGWFVRTPEVPKDGAKLCTIALSHYCEKARWALDLSPLRDTYIEDAHAPVFHYRPTVALTKDRKKTGLPVLVLPGVDPEGPPTVVQDSAEILQHLASLYPKELGHLYPPGDMGDKVRALE 127          
BLAST of EWM20757.1 vs. NCBI_GenBank
Match: gi|929756771|ref|XP_014153367.1| (hypothetical protein SARC_08141 [Sphaeroforma arctica JP610] >gi|906948709|gb|KNC79465.1| hypothetical protein SARC_08141 [Sphaeroforma arctica JP610])

HSP 1 Score: 129.798 bits (325), Expect = 2.027e-31
Identity = 109/335 (32.54%), Postives = 164/335 (48.96%), Query Frame = 0
Query:    3 HVLRTVGWFVRTPEVPKDGAKLCTIALSHYCEKARWALDLSPLRDTYIEDAHAPVFHYRPTVALTKDRKKTGLPVLVLPGVDPEGPPTVVQDSAEILQHLASLYPKELGHMYPPGDMGDKVRALERKLGEQLGPATRKIVYWEMWQGRQEGIDTCNALLERHYP---TAEARMWRLLEAKILPVMLKAMKIDETSTLAALEESREIFREVSALLDSEEGGKGGGAPAGPRRYLLGANRMTAADLTFAALAYPLINPPAFASLG----------LSGAEASPWAKRLAVITKEFRATPAGQHVLRLYGEERFPETSMAGKEPTGQLCLRTSNRDKW 324
            +VL  +  +        D   L TI++SH+CE+ARWALDLSPL D Y+ED+HAP  H   T  LT D + T  P++ LPG +      +++ S EIL++LA  YP E G +YP G   + +   E    + +G   RK  Y  +     E  D    L E + P    +   + R+L     PV+ K +K        + E+  E+ +++  ++D     K   A A  R YL+G +R TAADLTFA+LAYP + PP    +           L      P    +  + + ++ATPAG+  LR+Y   RF   S+ G    G++ +R S   +W
Sbjct:   21 YVLGNLMHYCNGSNEKPDKPLLVTISVSHFCERARWALDLSPLWDDYMEDSHAPGMHMPQTNRLT-DFQNTSTPMMKLPGTN-----QLIEGSDEILKYLAETYP-ECGFLYPHGHEIE-ILEWEHLFADTIGVLDRKWAYTHLLCMADE--DDQKRLTEMNGPFMAQSGGTLERVLFGMAWPVIGKQIK---RQFKLSKEKEAELVQQLDEIMD-----KVNRALADGREYLVG-DRFTAADLTFASLAYPFLAPPEMGDVSPCPTIGGKRYLEDLLQGPLKLYMERV-EGWQATPAGKLALRVYRNHRF---SVKGDVRAGEM-VRLSRNSEW 331          
BLAST of EWM20757.1 vs. NCBI_GenBank
Match: gi|1100138869|emb|SFE22276.1| (glutathione S-transferase [Nannocystis exedens])

HSP 1 Score: 121.709 bits (304), Expect = 5.659e-29
Identity = 105/284 (36.97%), Postives = 141/284 (49.65%), Query Frame = 0
Query:   23 KLCTIALSHYCEKARWALDLSPLRDTYIEDAHAPVFHYRPTVALTKDRKKTGLPVLVLP-GVDPEGPPTVVQDSAEILQHLASLYPKELGHMYPPGDMGDKVRALERKLGEQLGPATRKIVYWEMWQGRQEGIDTCNALLERHYPTAEARMWRLLEAKILPVMLKAMKIDETSTLAALEESREIFREVSALLDSEEGGKGGGAPAGPRRYLLGANRMTAADLTFAALAYPLINPPAFA----SLGLSGAEASPWAKRLAVITKEFRATPAGQHVLRLYGEERFP 301
            +L TI  SHYCEKARWALD + L   Y+E  H P+FH+  T  L    K+T +P L  P GV  E    +V+ + E L     L+P+E     PP  +  +V  L  +    LGPA R++ YW M+  R +       LL    P  E R+ RLL   +  +M++ +K+    T  A     EIF EV+  L            A  RRYL+G +R TAADLTFAAL  P +  P +        L+ A  + WA RL       R++PAG   LRL+ EER P
Sbjct:    9 RLITIGFSHYCEKARWALDRAGLE--YVEADHVPLFHFAAT--LPAGAKRT-VPALATPRGVLCES-TDIVRFADETLPAARKLFPEE-----PP--LRAEVERLVARFDALLGPAVRRVAYWHMFTDRGK----TRELLTSTGPAWERRLGRLLAVPMQAMMIRGLKLYPEPTARAQARLDEIFDEVAQTL------------ADGRRYLVG-DRFTAADLTFAALCGPALIAPQYGFPVPPQALTPATVN-WASRL-------RSSPAGAFALRLFAEERPP 254          
BLAST of EWM20757.1 vs. NCBI_GenBank
Match: gi|746980985|gb|KIF37585.1| (glutathione S-transferase [Hassallia byssoidea VB512170])

HSP 1 Score: 117.472 bits (293), Expect = 1.343e-27
Identity = 97/274 (35.40%), Postives = 135/274 (49.27%), Query Frame = 0
Query:   23 KLCTIALSHYCEKARWALDLSPLRDTYIEDAHAPVFHYRPTVALTKDRKKTGLPVLVLPGVDPEGPPTVVQDSAEILQHLASLYPKELGHMYPPG-DMGDKVRALERKLGEQLGPATRKIVYWEMWQGRQEGIDTCNALLERHYPTAEARMWRLLEAKILPVMLKAMKIDETSTLAALEESREIFREVSALLDSEEGGKGGGAPAGPRRYLLGANRMTAADLTFAALAYPLINPPAFASLGLSGAEASPWAKRLAVITKEFRATPAGQHVLRLY 295
            +L TI +SHYCEKARWAL  + L+ TY+E+AH P F YR  +A  +   K+  PVLV           V  DS +ILQ+L  L P     +YP    +  +V  LE    EQLGPATR+  Y+      Q           +  P+ E  ++ ++   + P++ KA  I+  S   A E+   +F +V+ LL            A  R YL+G  + +AAD+TFAALA P+I+PP      +            A     FR TPAG +VLRLY
Sbjct:    7 RLITIPVSHYCEKARWAL--TKLKLTYVEEAHMPPF-YR--LATNRVGGKST-PVLVTGD-------GVFTDSTDILQYLDKLVPDN-AKLYPSDFQLRRQVEELEDLFDEQLGPATRRWGYFYTINDSQ----FIKKRWTQGVPSFEEALFPVVYPLMRPIVRKAFNINSESATQAYEQINSVFEKVNELL------------ADGRSYLVG-EKFSAADITFAALAAPVISPPEHP---IKPRPLQELPDSFASKISAFRETPAGAYVLRLY 246          
BLAST of EWM20757.1 vs. NCBI_GenBank
Match: gi|1209854600|dbj|GAX40355.1| (putative glutathione S-transferase [Tolypothrix sp. NIES-4075])

HSP 1 Score: 117.087 bits (292), Expect = 2.127e-27
Identity = 95/274 (34.67%), Postives = 131/274 (47.81%), Query Frame = 0
Query:   23 KLCTIALSHYCEKARWALDLSPLRDTYIEDAHAPVFHYRPTVALTKDRKKTGLPVLVLPGVDPEGPPTVVQDSAEILQHLASLYPKELGHMYPPG-DMGDKVRALERKLGEQLGPATRKIVYWEMWQGRQEGIDTCNALLERHYPTAEARMWRLLEAKILPVMLKAMKIDETSTLAALEESREIFREVSALLDSEEGGKGGGAPAGPRRYLLGANRMTAADLTFAALAYPLINPPAFASLGLSGAEASPWAKRLAVITKEFRATPAGQHVLRLY 295
            +L TI +SHYCEKARWA  L+ L+ TY+E+ H P FH   T  L         PVLV           V  DS +ILQ+L  + P     +YP    +  +V  LE    EQLG ATR+  Y+      +        +  +  P+ E  ++ ++   + P++ KA  I   S   A E+ + IF +V+ LL            A  R YL+G N  +AAD+TFAALA P+I+PP      +           LA     FR TPAG +VLRLY
Sbjct:   12 RLITIPVSHYCEKARWA--LTKLKLTYVEEGHMPPFHRLATNRL----GGKSTPVLVTED-------GVFTDSTDILQYLDKIAPDN-AKLYPSDPQLRQQVEELEDLFDEQLGTATRRWGYFSTINDSK----LIQKIWTQDVPSFEKALFPVVYPFMRPLVRKAFNITPESAAQAYEQIKSIFEKVNELL------------ADGRIYLVGDN-FSAADITFAALAAPVISPPEHP---IKRRPLQDLPANLASEISAFRETPAGAYVLRLY 251          
BLAST of EWM20757.1 vs. NCBI_GenBank
Match: gi|873228707|emb|CEM15049.1| (unnamed protein product [Vitrella brassicaformis CCMP3155])

HSP 1 Score: 117.472 bits (293), Expect = 6.202e-27
Identity = 100/283 (35.34%), Postives = 149/283 (52.65%), Query Frame = 0
Query:   22 AKLCTIALSHYCEKARWALDLSPLRDTYIEDAHAPVFHYRPTVALTKDRKKTGLPVLVLPGVDPEGPPTVVQDSAEILQHLASLYPKELGHMYPPGDMGDKVRALERKLGEQLGPATRKIVYWEMWQGRQEGIDTCNALLERHYPTAEARMWRLLEAKILPVMLKAMKIDETSTLAALEESREIFREVSALLDSEEGGKGGGAPAGPRRYLLGANRMTAADLTFAALAYPLINPPAFASL-GLSGA-EASPWAKRLAVITKEFRATPAGQHVLRLYGEERFPE 302
            A+L +I +SH+ EK RWALDLS   D Y+EDAH P  H   T+  TK    T +PVLVL    P+G   V+ DS++IL  +A  +P EL ++Y P +  ++++A ER + E+L PA R + Y    +     I      +  H    E  M       +LPVM   M      +   L+E ++   +V  L++++         +  R+YL G++  TAADLTFAA+A  L+  P  + + G S + +ASP    L     + R+T AGQH L++Y   RFPE
Sbjct:   49 ARLISIPVSHFTEKVRWALDLS--GDDYVEDAHPPGMHAHYTLEATKG-ASTMVPVLVL----PDG--KVLTDSSDILDFIAEKHP-EL-NLY-PAEHKEEIKAFERIVNEKLAPAVRSMAYTIYGKDASAKIKIARVFMS-HGSVVENMMAPF----VLPVMAGIMTKAFGLSWDRLDEYQKEVMDVFTLVENK--------LSDGRQYLFGSS-FTAADLTFAAIANVLVGVPQMSGVFGQSESYDASP--PELEKRRGQLRSTKAGQHCLKVYETCRFPE 303          
BLAST of EWM20757.1 vs. NCBI_GenBank
Match: gi|1200363095|gb|OUU82439.1| (hypothetical protein CBC32_17670 [Proteobacteria bacterium TMED72])

HSP 1 Score: 117.472 bits (293), Expect = 7.808e-27
Identity = 105/283 (37.10%), Postives = 136/283 (48.06%), Query Frame = 0
Query:   21 GAKLCTIALSHYCEKARWALDLSPLRDTYIEDAHAPVFHYRPTVALTKDRKKTGLPVLVLPGVDPEGPPTVVQDSAEILQHLASLYPKELGHMYPPGDMGDKVRALERKLGEQLGPATRKIVYWEMWQGRQEGIDTCNALLERHYPTAEARMWRLLEAKILPVMLKAMKIDETSTLAALEESREIFREVSALLDSEEGGKGGGAPAGPRRYLLGANRMTAADLTFAALAYPLINPPAFASLG--LSGAEASPWAKRLAVITKEFRATPAGQHVLRLYGEERFP 301
             A+L TI  SHY E+ARWALDLSP+   Y EDAH P F       +T  RK    P LVLP  +      V+ DSA IL+ L  L+P+EL  +YP G   +++  LE +L   LG   R+  Y     G  E        L +     E  ++     +I   +++           A E  R +F  +S  L     G G       RRYL  A+  TAADLT AAL++PL+ PP +   G  LS  E      R      EFR+TPAGQH LR+Y E RFP
Sbjct:   54 SARLITIRYSHYNERARWALDLSPM--PYTEDAHPPGFAQFAIQDVTGGRKSAS-PALVLPDGE------VLDDSALILRRLHELFPEELSWLYPAG-YAEEILELEDELSLGLGAPIRQFAYAIALDG--ESYSLTRPYLTKESALIEKVLFYFGGRRISRAIVEIYNCRSACIPHAEEALRGVFETLSQRL-----GDG-------RRYLC-ADSFTAADLTLAALSWPLVFPPLWEESGIFLSYTEI---PDRWRKTVDEFRSTPAGQHALRMYREHRFP 308          
BLAST of EWM20757.1 vs. NCBI_GenBank
Match: gi|889960296|ref|WP_048866779.1| (glutathione S-transferase [Scytonema tolypothrichoides] >gi|750925700|gb|KIJ85513.1| glutathione S-transferase [Scytonema tolypothrichoides VB-61278])

HSP 1 Score: 115.546 bits (288), Expect = 9.159e-27
Identity = 93/277 (33.57%), Postives = 131/277 (47.29%), Query Frame = 0
Query:   23 KLCTIALSHYCEKARWALDLSPLRDTYIEDAHAPVFHYRPTVALTKDRKKTGLPVLVLPGVDPEGPPTVVQDSAEILQHLASLYPKELGHMYPPG-DMGDKVRALERKLGEQLGPATRKIVYWE-MWQGRQEGIDTCNALLERHYPTAEARMWRLLEAKILPVMLKAMKIDETSTLAALEESREIFREVSALLDSEEGGKGGGAPAGPRRYLLGANRMTAADLTFAALAYPLINPPAFASLGLSGAEASPWAKRLAVITKEFRATPAGQHVLRLYGE 297
            +L TI +SHYCEKARWA  L+ L+  YIE+ H P FH    +  T        PV++            + DS +IL +L  + P     +YP   +   +V  LE    EQLGPATR+  Y+  M   +      CN +     P  E  ++ ++   +  ++ + + I + S   A E+   IF +VS LL               R YL+G N  +AADLTFAALA P + PP      +S A       +L    K+FR TPAG  VLRLY +
Sbjct:   13 RLITIPVSHYCEKARWA--LTRLKLPYIEEPHMPPFH----LLATGRVGGKSTPVMLTEAGS-------LTDSTDILLYLDKVAPDN-AKLYPTNPNSRRQVEQLENLFNEQLGPATRRWAYFHTMNDSKLMQRGWCNGV-----PFVERVLFPVVFPTVRSIVRQKLNITQESASQAYEQINSIFEQVSELL------------TDGRTYLVG-NNFSAADLTFAALAAPAVRPPEHP---MSSASLEELPSKLVCEIKKFRETPAGAFVLRLYHD 254          
BLAST of EWM20757.1 vs. NCBI_GenBank
Match: gi|769920591|ref|WP_045055002.1| (glutathione S-transferase [Aliterella atlantica] >gi|768385472|gb|KJH71380.1| glutathione S-transferase [Aliterella atlantica CENA595])

HSP 1 Score: 115.161 bits (287), Expect = 1.049e-26
Identity = 100/282 (35.46%), Postives = 140/282 (49.65%), Query Frame = 0
Query:   23 KLCTIALSHYCEKARWALDLSPLRDTYIEDAHAPVFHYRPTVALTKDRKKTGLPVLVLPGVDPEGPPTVVQDSAEILQHLASLYPKELGHMYPPG-DMGDKVRALERKLGEQLGPATRKIVYWEMWQGRQEGIDTCNALLERHYPTAEARMWRLLEAKILPVMLKAMK----IDETSTLAALEESREIFREVSALLDSEEGGKGGGAPAGPRRYLLGANRMTAADLTFAALAYPLINPPAFASLGLSGAEASPWAKRLAVITKEFRATPAGQHVLRLYGEER 299
            +L TIA+SHYCEKARWALD   L+  Y+E++HAPVFH R T   T+      +PVLV           V  DS +IL H+ ++   E  H+YP    +  +V  LE     +LG      VY   W G    I    AL E  +     ++ ++      PVM + +K    +   S  ++L E R+IF  VS  L            +G R YL+G ++ TAADLTFAALA P++ P       +   +A    + +A + KE R T AG+  LRLY E+R
Sbjct:    3 RLITIAISHYCEKARWALDW--LKVPYVEESHAPVFH-RLT---TRRLGGKSVPVLVTES-------GVFTDSRDILHHIDAIATTE-RHLYPSDPQLRSQVEQLEELFDRKLG------VYVRQW-GYYYRIGDLEALQEM-WSNGATQLEQVGVKVAFPVMRQIVKRAYDVSAASAASSLTEVRQIFELVSQRL------------SGDRSYLVG-DKFTAADLTFAALASPVLRPKQHP---IQLTQAGKPNEEMAAVIKELRKTKAGKFALRLYQEQR 246          
The following BLAST results are available for this feature:
BLAST of EWM20757.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10
Match NameE-valueIdentityDescription
gi|585100630|gb|EWM20757.1|0.000e+0100.00hypothetical protein Naga_100518g2 [Nannochloropsi... [more]
gi|553183975|ref|XP_005854005.1|7.580e-8699.21glutathione s-transferase [Nannochloropsis gaditan... [more]
gi|929756771|ref|XP_014153367.1|2.027e-3132.54hypothetical protein SARC_08141 [Sphaeroforma arct... [more]
gi|1100138869|emb|SFE22276.1|5.659e-2936.97glutathione S-transferase [Nannocystis exedens][more]
gi|746980985|gb|KIF37585.1|1.343e-2735.40glutathione S-transferase [Hassallia byssoidea VB5... [more]
gi|1209854600|dbj|GAX40355.1|2.127e-2734.67putative glutathione S-transferase [Tolypothrix sp... [more]
gi|873228707|emb|CEM15049.1|6.202e-2735.34unnamed protein product [Vitrella brassicaformis C... [more]
gi|1200363095|gb|OUU82439.1|7.808e-2737.10hypothetical protein CBC32_17670 [Proteobacteria b... [more]
gi|889960296|ref|WP_048866779.1|9.159e-2733.57glutathione S-transferase [Scytonema tolypothricho... [more]
gi|769920591|ref|WP_045055002.1|1.049e-2635.46glutathione S-transferase [Aliterella atlantica] >... [more]
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Relationships

This CDS is a part of the following mRNA feature(s):

Feature NameUnique NameSpeciesType
rna9945rna9945Nannochloropsis gaditana (N. gaditana B-31)mRNA


Sequences
Synonyms
Publications