EWM21129.1, cds9543 (CDS) Nannochloropsis gaditana | NanDeSyn: Nannochloropsis Design & Synthesis Initiative

Overview


Name	EWM21129.1
Unique Name	cds9543
Type	CDS
Organism	Nannochloropsis gaditana (N. gaditana B-31)
Alignment location	KK037254.1:203637..203696 +
Alignment location	KK037254.1:203808..205448 +

Link to JBrowse

Properties


Property Name	Value
Protein id	EWM21129.1
Product	tryptophanyl-trna cytoplasmic
Orig transcript id	gnl\|cribi\|Naga_100041g13.3699.mrna
Gene	TrpRS
Gbkey	CDS

Mutants

Expression

No biomaterial libraries express this feature.

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
KK037254.1	supercontig	KK037254.1:203637..203696 +
KK037254.1	supercontig	KK037254.1:203808..205448 +

Analyses

This CDS is derived from or has results from the following analyses


Analysis Name	Date Performed
GO annotation for N. gaditana B31	2020-04-08
BLAST analysis for N. gaditana B-31	2020-04-07
InterPro analysis for N. gaditana B-31	2020-04-06
Gene prediction for N. gaditana B-31	2014-02-18

Annotated Terms

The following terms have been associated with this CDS:

Vocabulary: **Biological Process**
Term	Definition
GO:0006412	translation
GO:0006139	nucleobase-containing compound metabolic process
GO:0006418	tRNA aminoacylation for protein translation
GO:0006436	tryptophanyl-tRNA aminoacylation

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0004812	aminoacyl-tRNA ligase activity
GO:0004830	tryptophan-tRNA ligase activity
GO:0000166	nucleotide binding
GO:0005524	ATP binding

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm

Vocabulary: **INTERPRO**
Term	Definition
IPR018163	Thr/Ala-tRNA-synth_IIc_edit
IPR001412	aa-tRNA-synth_I_CS
IPR002305	aa-tRNA-synth_Ic
IPR014729	Rossmann-like_a/b/a_fold
IPR002306	Trp-tRNA-ligase

Homology

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BLAST of EWM21129.1 vs. NCBI_GenBank
Match: gi|585101196|gb|EWM21129.1| (tryptophanyl-trna cytoplasmic [Nannochloropsis gaditana])

HSP 1 Score: 1171.76 bits (3030), Expect = 0.000e+0
Identity = 566/566 (100.00%), Postives = 566/566 (100.00%), Query Frame = 0

Query:    1 MASPSELRTPQAVTSALIVKGALANVLGFPIGGTTIGTHYSEGHRVRLTVQIGQAPDPTETQFIQVAETINTILAANQPVHVFKLARHRAQTSYKNAMYNRHAVPESITELTIAYIEKIAFSCTAEAVVASTGMVGAITINKTKFRSAKKELEIQYTVSTPSESGPAVLAVAKEDDETLPSSEAISLLNSDTVRQASGGKGNEKGGNGGSIGSASGEGGQVVTPWEVEGDQEIDYDKLIKSFGSMRITESLVSRIERLTNRKCHRFLRRGLFFSHRDLTQLLDLYEKGTKFYLYTGRGPSSESLHLGHLIPFHFTRYLQEAFDVPLVIQLTDDEKFQFKQGLALEECHRLAYENAKDVIACGFDVKKTFIFSDLDYIQHLYPTVLKIQKLVTFNQARGIFGFTESDSCGKIAFPAVQAAPSFPSSFPVPLKGCLDMPCLIPCAIDQDAYFRMTRDVAPRLGCKKPSLIHSKFFPGLEVSGCGGAKIERAIMRGAGLQLFEAGYNCDCINLRWDISEGFFFKPCVLTDERDIPMYLPTPPFPLPPVTVPPRAKAARCPPRPSSRPSM 566
            MASPSELRTPQAVTSALIVKGALANVLGFPIGGTTIGTHYSEGHRVRLTVQIGQAPDPTETQFIQVAETINTILAANQPVHVFKLARHRAQTSYKNAMYNRHAVPESITELTIAYIEKIAFSCTAEAVVASTGMVGAITINKTKFRSAKKELEIQYTVSTPSESGPAVLAVAKEDDETLPSSEAISLLNSDTVRQASGGKGNEKGGNGGSIGSASGEGGQVVTPWEVEGDQEIDYDKLIKSFGSMRITESLVSRIERLTNRKCHRFLRRGLFFSHRDLTQLLDLYEKGTKFYLYTGRGPSSESLHLGHLIPFHFTRYLQEAFDVPLVIQLTDDEKFQFKQGLALEECHRLAYENAKDVIACGFDVKKTFIFSDLDYIQHLYPTVLKIQKLVTFNQARGIFGFTESDSCGKIAFPAVQAAPSFPSSFPVPLKGCLDMPCLIPCAIDQDAYFRMTRDVAPRLGCKKPSLIHSKFFPGLEVSGCGGAKIERAIMRGAGLQLFEAGYNCDCINLRWDISEGFFFKPCVLTDERDIPMYLPTPPFPLPPVTVPPRAKAARCPPRPSSRPSM
Sbjct:    1 MASPSELRTPQAVTSALIVKGALANVLGFPIGGTTIGTHYSEGHRVRLTVQIGQAPDPTETQFIQVAETINTILAANQPVHVFKLARHRAQTSYKNAMYNRHAVPESITELTIAYIEKIAFSCTAEAVVASTGMVGAITINKTKFRSAKKELEIQYTVSTPSESGPAVLAVAKEDDETLPSSEAISLLNSDTVRQASGGKGNEKGGNGGSIGSASGEGGQVVTPWEVEGDQEIDYDKLIKSFGSMRITESLVSRIERLTNRKCHRFLRRGLFFSHRDLTQLLDLYEKGTKFYLYTGRGPSSESLHLGHLIPFHFTRYLQEAFDVPLVIQLTDDEKFQFKQGLALEECHRLAYENAKDVIACGFDVKKTFIFSDLDYIQHLYPTVLKIQKLVTFNQARGIFGFTESDSCGKIAFPAVQAAPSFPSSFPVPLKGCLDMPCLIPCAIDQDAYFRMTRDVAPRLGCKKPSLIHSKFFPGLEVSGCGGAKIERAIMRGAGLQLFEAGYNCDCINLRWDISEGFFFKPCVLTDERDIPMYLPTPPFPLPPVTVPPRAKAARCPPRPSSRPSM 566

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BLAST of EWM21129.1 vs. NCBI_GenBank
Match: gi|299472643|emb|CBN78295.1| (Tryptophan--tRNA ligase [Ectocarpus siliculosus])

HSP 1 Score: 570.466 bits (1469), Expect = 0.000e+0
Identity = 286/483 (59.21%), Postives = 349/483 (72.26%), Query Frame = 0

Query:    1 MASPSELRTPQAVTSALIVKGALANVLGFPIGGTTIGTHYSEGHRVRLTVQIGQAPDPTETQFIQVAETINTILAANQPVHVFKLARHRAQTSYKNAMYNRHAVPESITELTIAYIEKIAFSCTAEAVVASTGMVGAITINKTKFRSAKKELEIQYTVSTPSESGPAVLAVAKEDDETLPSSEAISLLNSDTVRQASGGKGNEKGGNGGSIGSASGEGGQVVTPWEVEGDQEIDYDKLIKSFGSMRITESLVSRIERLTNRKCHRFLRRGLFFSHRDLTQLLDLYEKGTKFYLYTGRGPSSESLHLGHLIPFHFTRYLQEAFDVPLVIQLTDDEKFQFKQGLALEECHRLAYENAKDVIACGFDVKKTFIFSDLDYIQHLYPTVLKIQKLVTFNQARGIFGFTESDSCGKIAFPAVQAAPSFPSSFPVPLKGCLDMPCLIPCAIDQDAYFRMTRDVAPRLGCKKPSLIHSKFFPGLEVSGCGG 483
            MA+  + RTP   T+  IVKGAL  V+GFPIG +TIG+  SEG++ R+TV+ G+A  PT  Q  +V + +N  +A +Q      + R  A+++Y +AMY+    P  + EL++ YIE +         + STG VG +++ K KFR  K+ELE+ + V  PS         A       PS+E + LLN+   R  S   G E G      G A+ EGGQ VTPWEVE D E+DY+KL+KSFGS  ITE +V+R+ER+T RK HRFLRRGLFFSHRDLTQ+LDLYE G KF+LYTGRGPSS++LHLGHL+PFHFT+YLQEAF VPLV+QLTDDEKF FK  L LEECHRLA+ENAKD+IACGFD + TFIFSDLDYIQH+YP VLKIQKLVT+NQ RGIFGFTES + G  AFPAVQAAPSF SSFP+PL G  D PCLIPCAIDQDAYFRMTRDVAPR+  +KP+LIHS+FFPGL+  G GG
Sbjct:    1 MATTVDPRTPTMTTALEIVKGALCAVIGFPIGKSTIGSRISEGNKARITVKTGKAEAPTAAQMQKVVDVVNEAIAKDQACFRLTMPRKDAESTYGSAMYDVVEPPAQVEELSLLYIEGLVLHSQLHPCLPSTGPVGKVSVTKQKFRVNKQELEVMFDVE-PSTDT-PTDDTATPPQCVPPSAETVVLLNASEAR-VSADDGVEGGVEES--GKAAAEGGQKVTPWEVEADGEVDYEKLVKSFGSSLITEDIVARVERVTGRKAHRFLRRGLFFSHRDLTQILDLYEAGKKFFLYTGRGPSSDALHLGHLVPFHFTKYLQEAFKVPLVVQLTDDEKFLFKPELKLEECHRLAFENAKDIIACGFDPESTFIFSDLDYIQHMYPVVLKIQKLVTYNQVRGIFGFTESHNIGCQAFPAVQAAPSFSSSFPIPLGGTPDRPCLIPCAIDQDAYFRMTRDVAPRMKLRKPALIHSRFFPGLQ--GPGG 476

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BLAST of EWM21129.1 vs. NCBI_GenBank
Match: gi|873226335|emb|CEM21761.1| (unnamed protein product [Vitrella brassicaformis CCMP3155])

HSP 1 Score: 487.649 bits (1254), Expect = 3.792e-163
Identity = 255/486 (52.47%), Postives = 321/486 (66.05%), Query Frame = 0

Query:    8 RTPQAVTSALIVKGALANVLGFPIGGTTIGTHYSEGHRVRLTVQIGQA-PDPTETQFIQVAETINTILAANQPVHVFKLARHRAQTSYKNAMYNRHAVPESITELTIAYIEKIAFSCTAEAVVASTGMVGAITINKTKFRSAKKELEIQYTVS-----TPSESGPAVLAVAKEDDET------LPSSEAISLLNSDTVRQASGGKGNEKGGNGGSI--GSASGEGGQVVTPWEVEGDQE--IDYDKLIKSFGSMRITESLVSRIERLTNRKCHRFLRRGLFFSHRDLTQLLDLYEKGTKFYLYTGRGPSSESLHLGHLIPFHFTRYLQEAFDVPLVIQLTDDEKFQFKQGLALEECHRLAYENAKDVIACGFDVKKTFIFSDLDYIQHLYPTVLKIQKLVTFNQARGIFGFTESDSCGKIAFPAVQAAPSFPSSFPVPLKGCLDMPCLIPCAIDQDAYFRMTRDVAPRLGCKKPSLIHSKFFPGLE 477
            R P   T+  IVKGAL   L  P   +T    + +G  +R  + +G    +P E    ++ E +N  +AAN P+ VF +AR  A+T+Y  AMY+   VP+S+TEL + +IEK   +     V  STG  G I I K K  S K  LE+ + ++     T  ++ P     A  +D+       LP ++      +D   + +G   N +         G+ +G GGQ VTPWEVE D+E  IDYDKLI  FG  +ITE  +SRIERLT ++ HRFLRRGLFFSHRDL  LL+ YE+G  FYLYTGRGPSSE+LHLGHL+PF FT++LQ+AF+VPLVIQLTDDEKF FK+ L +EEC RLAYENAKD++A GFD  KTFIFSDLDYIQ +YP VL+IQK VT+NQ+RGIFGFTESD+ GK AFPAVQAAP+FP+ FP       D+ CLIP AIDQD YFRMTRDVAPRLG  KP+LIHS+F P L+
Sbjct:    7 RHPLLDTALHIVKGALVVALRIPTARSTKSVDFKKGLPLRGAITVGPVTSEPNEEDKRRIQELVNDKVAANVPIRVFAVARQLAETTYGEAMYDDFGVPKSVTELRLVFIEKWNINANIFPVAQSTGACGRIDITKWKHNSNKNSLEVSFDLTPTEAYTDEQNVPLATEEALANDQVPAREMVLPPADVAGTHGTDEATETNGNPQNSQQHQQQHDPEGNPTG-GGQRVTPWEVEADEETGIDYDKLIIQFGCSKITEDHISRIERLTKQRAHRFLRRGLFFSHRDLDMLLNAYEQGEPFYLYTGRGPSSEALHLGHLVPFWFTKWLQDAFNVPLVIQLTDDEKFLFKENLTVEECKRLAYENAKDILAIGFDKDKTFIFSDLDYIQTMYPLVLQIQKKVTYNQSRGIFGFTESDNIGKSAFPAVQAAPAFPACFPAMFGNRKDIMCLIPQAIDQDPYFRMTRDVAPRLGFNKPALIHSRFVPALQ 491

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BLAST of EWM21129.1 vs. NCBI_GenBank
Match: gi|294952951|ref|XP_002787529.1| (IFP53, putative [Perkinsus marinus ATCC 50983] >gi|239902535|gb|EER19325.1| IFP53, putative [Perkinsus marinus ATCC 50983])

HSP 1 Score: 461.07 bits (1185), Expect = 3.832e-153
Identity = 246/475 (51.79%), Postives = 313/475 (65.89%), Query Frame = 0

Query:    8 RTPQAVTSALIVKGALANVLGFPIGGTTIGTHYSEGHRVRLTVQIGQAPDPTETQFIQVAETINTILAANQPVHVFK-LARHRAQTSYKNAMYNRHAVPESITELTIAYIEKIAFSCTAEAVVASTGMVGAITINKTKFRSAKKELEIQYTVSTPSESGPAVLAVAKEDD--ETLPSSE-AISLLNSDTVRQASGGKGNEKGGNGGSIGSASGEGGQVVTPWEVEG-DQEIDYDKLIKSFGSMRITESLVSRIERLTNRKCHRFLRRGLFFSHRDLTQLLDLYEKGTKFYLYTGRGPSSESLHLGHLIPFHFTRYLQEAFDVPLVIQLTDDEKFQFKQGLALEECHRLAYENAKDVIACGFDVKKTFIFSDLDYIQHLYPTVLKIQKLVTFNQARGIFGFTESDSCGKIAFPAVQAAPSFPSSFPVPLKGCLDMPCLIPCAIDQDAYFRMTRDVAPRLGCKKPSLIHSKFFPGLE 477
            R+P    +  ++KGA+  VL  P G TT      EG   +LT+      +PTE Q   +   ++  +  N P  VF  + R  A+  Y + MY+   VP+S+ EL + Y+E+   +C    +V STG++G I + K K+ + K  LEI +TV  P +    V  +A+ED   E LP  + A+  +  + + Q             G +G + G   Q VTPWEVEG D+ IDYDKLI+ FG   I + L+ R+E+LT +K HRFLRRGLFFSHRDL  LLD YE+G  FYLYTGRGPSSESLHLGHL+PF FT++LQ+ FDVPLVIQLTDDEKF FK  L+LEE HRLAYENAKD+IACGFD+ KTFIFSDLDY+  +YP V KIQKLVT+NQARG FGFT SDS  K +F A+QA+PSF ++FP       D+ CLIP AIDQD YFR+TRDVAPR+G  KP+LIHSKFFP L+
Sbjct:    7 RSPAFDAALHVIKGAVCTVLRIPTGRTTDRVSPHEGGG-KLTLN-AIKDEPTEEQKELIVTNVHNKVEENAPFKVFTGVPRDLAEKKYFDTMYDSFKVPDSVKELRLVYLEQWNLNCNVHPIVKSTGLLGDINLTKWKYSAKKSTLEISFTVDPPCD----VFEMAEEDSNVEDLPPLDIAVPYVPDEQLSQ------------DGVLGVSEG---QKVTPWEVEGSDEGIDYDKLIRDFGCSPIDQKLIDRMEKLTGKKAHRFLRRGLFFSHRDLGILLDKYERGIPFYLYTGRGPSSESLHLGHLVPFQFTKWLQDTFDVPLVIQLTDDEKFFFKDYLSLEEAHRLAYENAKDIIACGFDMDKTFIFSDLDYMGTMYPNVCKIQKLVTYNQARGAFGFTGSDSVAKSSFCAIQASPSFSTTFPSIFGDRKDIMCLIPQAIDQDPYFRVTRDVAPRMGMLKPALIHSKFFPALQ 460

[Close]

BLAST of EWM21129.1 vs. NCBI_GenBank
Match: gi|294871822|ref|XP_002766059.1| (IFP53, putative [Perkinsus marinus ATCC 50983] >gi|239866624|gb|EEQ98776.1| IFP53, putative [Perkinsus marinus ATCC 50983])

HSP 1 Score: 456.447 bits (1173), Expect = 4.941e-144
Identity = 245/476 (51.47%), Postives = 312/476 (65.55%), Query Frame = 0

Query:    8 RTPQAVTSALIVKGALANVLGFPIGGTT--IGTHYSEGHRVRLTVQIGQAPDPTETQFIQVAETINTILAANQPVHVFK-LARHRAQTSYKNAMYNRHAVPESITELTIAYIEKIAFSCTAEAVVASTGMVGAITINKTKFRSAKKELEIQYTVSTPSESGPAVLAVAKEDD--ETLPSSE-AISLLNSDTVRQASGGKGNEKGGNGGSIGSASGEGGQVVTPWEVEG-DQEIDYDKLIKSFGSMRITESLVSRIERLTNRKCHRFLRRGLFFSHRDLTQLLDLYEKGTKFYLYTGRGPSSESLHLGHLIPFHFTRYLQEAFDVPLVIQLTDDEKFQFKQGLALEECHRLAYENAKDVIACGFDVKKTFIFSDLDYIQHLYPTVLKIQKLVTFNQARGIFGFTESDSCGKIAFPAVQAAPSFPSSFPVPLKGCLDMPCLIPCAIDQDAYFRMTRDVAPRLGCKKPSLIHSKFFPGL 476
            R+P    +  ++KGA+  VL  P G TT  +  H   G   +LT+      +PTE Q   +A  +   +  N P  VF  + R  A+  Y + MY+   VPES+ EL + Y+E+   +C    ++ STG++G + +   K+ + K  LEI +TV    E    V  +A+ED   E LPS + A+  +  + + Q               +G ++    Q VTPWEVEG D+ IDYDKLI+ FG   I + L+ R+E+LT +K HRFLRRGLFFSHRDL  LLD YE+G  FYLYTGRGPSSESLHLGHL+PF FT++LQ+ FDVPLVIQLTDDEKF FK  L+LEE HRLAYENAKD+IACGFD+ KTFIFSDLDY+  +YP V KIQKLVT+NQARG FGFT SDS GK +F A+QA+PSF ++FP       D+ CLIP AIDQD YFR+TRDVAPR+G  KP+LIHSKFFP L
Sbjct:    5 RSPAFDAALHVIKGAVCTVLRIPTGRTTERVSPHLGGG---KLTLN-SIKEEPTEEQKELIATNVYNKVEENAPFKVFTGVPRDLAERKYFDTMYDSFKVPESVKELRLVYLEQWNLNCNVHPIIKSTGLLGEVNLTHWKYSAKKATLEISFTV----EPSCDVFEMAEEDGNVEDLPSLDIAVPYVPDEQLDQ------------NRVLGVSNC---QKVTPWEVEGSDEGIDYDKLIRDFGCSPIDQKLIDRMEKLTGKKAHRFLRRGLFFSHRDLGILLDKYERGIPFYLYTGRGPSSESLHLGHLVPFQFTKWLQDTFDVPLVIQLTDDEKFFFKDYLSLEEAHRLAYENAKDIIACGFDMDKTFIFSDLDYMGTMYPNVCKIQKLVTYNQARGAFGFTGSDSVGKSSFCAIQASPSFSTTFPSIFGDRKDIMCLIPQAIDQDPYFRVTRDVAPRMGMLKPALIHSKFFPAL 457

[Close]

BLAST of EWM21129.1 vs. NCBI_GenBank
Match: gi|294941734|ref|XP_002783213.1| (IFP53, putative [Perkinsus marinus ATCC 50983] >gi|239895628|gb|EER15009.1| IFP53, putative [Perkinsus marinus ATCC 50983])

HSP 1 Score: 454.907 bits (1169), Expect = 1.927e-142
Identity = 246/481 (51.14%), Postives = 315/481 (65.49%), Query Frame = 0

Query:    8 RTPQAVTSALIVKGALANVLGFPIGGTT--IGTHYSEGHRVRLTVQIGQAPDPTETQFIQVAETINTILAANQPVHVFK-LARHRAQTSYKNAMYNRHAVPESITELTIAYIEKIAFSCTAEAVVASTGMVGAITINKTKFRSAKKELEIQYTVSTPSESGPAVLAVAKEDD--ETLPSSE-AISLLNSDTVRQASGG----KGNEKGGNGGSIGSASGEGGQVVTPWEVEG-DQEIDYDKLIKSFGSMRITESLVSRIERLTNRKCHRFLRRGLFFSHRDLTQLLDLYEKGTKFYLYTGRGPSSESLHLGHLIPFHFTRYLQEAFDVPLVIQLTDDEKFQFKQGLALEECHRLAYENAKDVIACGFDVKKTFIFSDLDYIQHLYPTVLKIQKLVTFNQARGIFGFTESDSCGKIAFPAVQAAPSFPSSFPVPLKGCLDMPCLIPCAIDQDAYFRMTRDVAPRLGCKKPSLIHSKFFPGLE 477
            R+P    +  ++KGA+  VL  P G TT  +  H   G   +LT+      +PTE Q   +A  +   +  N P  VF  + R  A+  Y + MY+   VPES+ EL + Y+E+   +C    ++ STG++G + +   K+ + K  LEI +T+    E    V  +A+ED   E LPS + A+  +   T+     G       E+      +G ++    Q VTPWEVEG D+ IDYDKLI+ FG   I + L+ R+E+LT +K HRFLRRGLFFSHRDL  LLD YE+G  FYLYTGRGPSSESLHLGHL+PF FT++LQ+ FDVPLVIQLTDDEKF FK  L+LEE HRLAYENAKD+IACGFD+ KTFIFSDLDY+  +YP V KIQKLVT+NQARG FGFT SDS GK +F A+QA+PSF ++FP       D+ CLIP AIDQD YFR+TRDVAPR+G  KP+LIHSKFFP L+
Sbjct:    5 RSPAFDAALHVIKGAVCTVLRIPTGRTTERVSPHLGGG---KLTLN-SIKEEPTEEQKELIATNVYNKVEENAPFKVFTGVPRDLAERKYFDTMYDSFKVPESVKELRLVYLEQWNLNCNVHPIIKSTGLLGEVNLTHWKYSAKKATLEISFTL----EPSCDVFEMAEEDGNVEDLPSLDIAVPYVPDGTITYHFKGVIVVTLAEQLDQNRVLGVSNC---QKVTPWEVEGSDEGIDYDKLIRDFGCSPIDQKLIDRMEKLTGKKAHRFLRRGLFFSHRDLGILLDKYERGIPFYLYTGRGPSSESLHLGHLVPFQFTKWLQDTFDVPLVIQLTDDEKFFFKDYLSLEEAHRLAYENAKDIIACGFDMDKTFIFSDLDYMGTMYPNVCKIQKLVTYNQARGAFGFTGSDSVGKSSFCAIQASPSFSTTFPSIFGDRKDIMCLIPQAIDQDPYFRVTRDVAPRMGMLKPALIHSKFFPALQ 474

[Close]

BLAST of EWM21129.1 vs. NCBI_GenBank
Match: gi|401401570|ref|XP_003881044.1| (tryptophanyl-tRNAsynthetase,related [Neospora caninum Liverpool] >gi|325115456|emb|CBZ51011.1| tryptophanyl-tRNAsynthetase,related [Neospora caninum Liverpool] >gi|820692574|tpe|CEL68316.1| TPA: Tryptophanyl-tRNA synthetase, related [Neospora caninum Liverpool])

HSP 1 Score: 427.172 bits (1097), Expect = 1.067e-139
Identity = 231/494 (46.76%), Postives = 300/494 (60.73%), Query Frame = 0

Query:    8 RTPQAVTSALIVKGALANVLGFPIGGTTIGTHYSEGHRVRLTVQ-IGQAPDPTETQFIQVAETINTILAANQPVHVFKLARHRAQTSYKNAMYNRHAVPESITELTIAYIEKIAFSCTAEAVVASTGMVGAITINKTKFRSAKKELEIQYTVSTPSESG--PAVLAVAKEDDETLPSSEAISLLNSDTVRQASGGKGNEKGGNGGSIGSASGEGGQVVTPWEVEGDQE-IDYDKLIKSFGSMRITESLVSRIERLTNRKCHRFLRRGLFFSHRDLTQLLDLYEK--------------------GTKFYLYTGRGPSSESLHLGHLIPFHFTRYLQEAFDVPLVIQLTDDEKFQFKQGLALEECHRLAYENAKDVIACGFDVKKTFIFSDLDYIQHLYPTVLKIQKLVTFNQARGIFGFTESDSCGKIAFPAVQAAPSFPSSFPVPLKGCLDMPCLIPCAIDQDAYFRMTRDVAPRLGCKKPSLIHSKFFPGLE 477
            RTP    +  IVKGA    L    G TT    +S+ ++  +T+  +   P  +E + ++        +  N P  VF + R  A   Y+ +  +   +P ++ E+ +  + +   +      + STG +  I     KF + K++L +   V  P+++G  PAV    K   E LP+           V   SG +G++ G +G        +  Q VTPW+V+ ++  IDYDKL+K FG   IT  L+SRIE LT ++ H  LRRG+FFSHRDL  LLD  EK                    G  FYLYTGRGPSSE+LH+GHL+PF FT+YLQ+ FDVPLVIQLTDDEKF FK+ L LEE HRLA+ENAKD+IACGFD  KTFIFSDL YIQHLYP +L+IQK VT+NQ RG+FGF ESD+ GK AFPAVQAAPSFP++FP+   G  D+ CLIP AIDQD YFRMTRDVAPRLG  KP+LIHS+F P L+
Sbjct:    4 RTPYQDAALAIVKGAACIALSLKTGRTTRNVKFSKDNKCSITLGPVDNQPTASEEELLKT--LTQQKIEENCPFRVFTIPREVATRMYQESYLDEFGIPANVKEVRLVVLPEWNINANIYPTLKSTGQIAEIRFETVKFVAEKRQLCLTLQV-VPADNGDAPAVAVEEKVAPEDLPTLS--------QVLPPSGVEGDDLGNDG--------QDDQTVTPWDVQANEGGIDYDKLLKKFGCSAITPQLISRIEALTGKRAHHLLRRGIFFSHRDLNLLLDAIEKRQANLASEKREDGAGADAFSGPAFYLYTGRGPSSEALHIGHLVPFMFTKYLQDVFDVPLVIQLTDDEKFLFKENLTLEETHRLAFENAKDIIACGFDPDKTFIFSDLSYIQHLYPVILEIQKKVTYNQVRGLFGFVESDNIGKSAFPAVQAAPSFPTAFPMIFGGKKDVRCLIPQAIDQDPYFRMTRDVAPRLGLLKPALIHSRFIPALQ 478

[Close]

BLAST of EWM21129.1 vs. NCBI_GenBank
Match: gi|820700112|tpe|CEL75845.1| (TPA: tryptophanyl-tRNA synthetase, putative [Toxoplasma gondii VEG])

HSP 1 Score: 410.994 bits (1055), Expect = 1.711e-133
Identity = 227/492 (46.14%), Postives = 288/492 (58.54%), Query Frame = 0

Query:    8 RTPQAVTSALIVKGALANVLGFPIGGTTIGTHYSEGHRVRLTVQIGQAPDPTETQFIQVAETINTILAANQPVHVFKLARHRAQTSYKNAMYNRHAVPESITELTIAYIEKIAFSCTAEAVVASTGMVGAITINKTKFRSAKKELEIQ-YTVSTPSESGPAVLAVAKEDDETLPSSEAISLLNSDTVRQASGGKGNEKGGNGGSIGSASGEGGQVVTPWEVEGDQE-IDYDKLIKSFGSMRITESLVSRIERLTNRKCHRFLRRGLFFSHRDLTQLLDLYEK--------------------GTKFYLYTGRGPSSESLHLGHLIPFHFTRYLQEAFDVPLVIQLTDDEKFQFKQGLALEECHRLAYENAKDVIACGFDVKKTFIFSDLDYIQHLYPTVLKIQKLVTFNQARGIFGFTESDSCGKIAFPAVQAAPSFPSSFPVPLKGCLDMPCLIPCAIDQDAYFRMTRDVAPRLGCKKPSLIHSKFFPGLE 477
            RTP    +  IVKGA    L      TT    +S+ +R  +T+   +  +PT  +   +       +  N P  VF + R  A   Y+ +  +   +P ++ E+ +  + +   +     V+ STG +  I     KF   KK+L +  + V  P E  PA+    K   E +PS   +  L    V                      G+  Q +TPW+V+  +  IDY+KL+K FG   IT  L+SRIE LT ++ H  LRRG+FFSHRDL  LLD  EK                    G  FYLYTGRGPSSE+LH+GHL+PF FT+YLQ+ FDVPLVIQLTDDEKF FK  L LEE HRLA+ENAKD+IACGFD  KTFIFSDL YIQHLYP +L+IQK VT+NQ RG+FGF ESD+ GK AFPAVQAAPSFP++FP+   G  D+ CLIP AIDQD YFRMTRDVAPRLG  KP+LIHS+F P L+
Sbjct:    4 RTPFQDAALSIVKGAACIALSLKTARTTQNVKFSKDNRCSITLGPIEV-EPTAAEEALLKTLTKQKIEENCPFRVFTIPRDLATRMYQESYLDEFGIPANVKEVRLVVLPEWNINANMYPVLKSTGQIADIQFESVKFNEEKKQLCLSLHVVPGPDEQSPALAEEEKLGPEEIPSRAQV--LPPSGVEGDD---------------FGEGDDDQTITPWDVQAAEGGIDYNKLLKKFGCSSITPELISRIEALTGKRAHHLLRRGIFFSHRDLNLLLDSLEKRQARLASGKREDGPGSEGLSGPGFYLYTGRGPSSEALHIGHLVPFMFTKYLQDVFDVPLVIQLTDDEKFLFKDSLTLEETHRLAFENAKDIIACGFDPDKTFIFSDLSYIQHLYPVILEIQKKVTYNQVRGLFGFVESDNVGKSAFPAVQAAPSFPTAFPMIFGGKKDVRCLIPQAIDQDPYFRMTRDVAPRLGLLKPALIHSRFIPALQ 477

[Close]

BLAST of EWM21129.1 vs. NCBI_GenBank
Match: gi|672573471|gb|KFH12174.1| (tryptophanyl-tRNA synthetase [Toxoplasma gondii MAS])

HSP 1 Score: 410.994 bits (1055), Expect = 1.963e-132
Identity = 227/492 (46.14%), Postives = 288/492 (58.54%), Query Frame = 0

Query:    8 RTPQAVTSALIVKGALANVLGFPIGGTTIGTHYSEGHRVRLTVQIGQAPDPTETQFIQVAETINTILAANQPVHVFKLARHRAQTSYKNAMYNRHAVPESITELTIAYIEKIAFSCTAEAVVASTGMVGAITINKTKFRSAKKELEIQ-YTVSTPSESGPAVLAVAKEDDETLPSSEAISLLNSDTVRQASGGKGNEKGGNGGSIGSASGEGGQVVTPWEVEGDQE-IDYDKLIKSFGSMRITESLVSRIERLTNRKCHRFLRRGLFFSHRDLTQLLDLYEK--------------------GTKFYLYTGRGPSSESLHLGHLIPFHFTRYLQEAFDVPLVIQLTDDEKFQFKQGLALEECHRLAYENAKDVIACGFDVKKTFIFSDLDYIQHLYPTVLKIQKLVTFNQARGIFGFTESDSCGKIAFPAVQAAPSFPSSFPVPLKGCLDMPCLIPCAIDQDAYFRMTRDVAPRLGCKKPSLIHSKFFPGLE 477
            RTP    +  IVKGA    L      TT    +S+ +R  +T+   +  +PT  +   +       +  N P  VF + R  A   Y+ +  +   +P ++ E+ +  + +   +     V+ STG +  I     KF   KK+L +  + V  P E  PA+    K   E +PS   +  L    V                      G+  Q +TPW+V+  +  IDY+KL+K FG   IT  L+SRIE LT ++ H  LRRG+FFSHRDL  LLD  EK                    G  FYLYTGRGPSSE+LH+GHL+PF FT+YLQ+ FDVPLVIQLTDDEKF FK  L LEE HRLA+ENAKD+IACGFD  KTFIFSDL YIQHLYP +L+IQK VT+NQ RG+FGF ESD+ GK AFPAVQAAPSFP++FP+   G  D+ CLIP AIDQD YFRMTRDVAPRLG  KP+LIHS+F P L+
Sbjct:   87 RTPFQDAALSIVKGAACIALSLKTARTTQNVKFSKDNRCSITLGPIEV-EPTAAEEALLKTLTKQKIEENCPFRVFTIPRDLATRMYQESYLDEFGIPANVKEVRLVVLPEWNINANMYPVLKSTGQIADIQFESVKFNEEKKQLCLSLHVVPGPDEQSPALAEEEKLGPEEIPSRAQV--LPPSGVEGDD---------------FGEGDDDQTITPWDVQAAEGGIDYNKLLKKFGCSSITPDLISRIEALTGKRAHHLLRRGIFFSHRDLNLLLDSLEKRQARLASGKREDGPGSEGLSGPGFYLYTGRGPSSEALHIGHLVPFMFTKYLQDVFDVPLVIQLTDDEKFLFKDSLTLEETHRLAFENAKDIIACGFDPDKTFIFSDLSYIQHLYPVILEIQKKVTYNQVRGLFGFVESDNVGKSAFPAVQAAPSFPTAFPMIFGGKKDVRCLIPQAIDQDPYFRMTRDVAPRLGLLKPALIHSRFIPALQ 560

[Close]

BLAST of EWM21129.1 vs. NCBI_GenBank
Match: gi|523573065|gb|EPR59882.1| (tryptophanyl-tRNA synthetase (TrpRS2) [Toxoplasma gondii GT1] >gi|557737870|gb|ESS33818.1| tryptophanyl-tRNA synthetase (TrpRS2) [Toxoplasma gondii VEG] >gi|672252419|gb|KFG28938.1| tryptophanyl-tRNA synthetase (TrpRS2) [Toxoplasma gondii p89] >gi|672298594|gb|KFG62553.1| tryptophanyl-tRNA synthetase (TrpRS2) [Toxoplasma gondii RUB])

HSP 1 Score: 410.609 bits (1054), Expect = 2.487e-132
Identity = 227/492 (46.14%), Postives = 288/492 (58.54%), Query Frame = 0

Query:    8 RTPQAVTSALIVKGALANVLGFPIGGTTIGTHYSEGHRVRLTVQIGQAPDPTETQFIQVAETINTILAANQPVHVFKLARHRAQTSYKNAMYNRHAVPESITELTIAYIEKIAFSCTAEAVVASTGMVGAITINKTKFRSAKKELEIQ-YTVSTPSESGPAVLAVAKEDDETLPSSEAISLLNSDTVRQASGGKGNEKGGNGGSIGSASGEGGQVVTPWEVEGDQE-IDYDKLIKSFGSMRITESLVSRIERLTNRKCHRFLRRGLFFSHRDLTQLLDLYEK--------------------GTKFYLYTGRGPSSESLHLGHLIPFHFTRYLQEAFDVPLVIQLTDDEKFQFKQGLALEECHRLAYENAKDVIACGFDVKKTFIFSDLDYIQHLYPTVLKIQKLVTFNQARGIFGFTESDSCGKIAFPAVQAAPSFPSSFPVPLKGCLDMPCLIPCAIDQDAYFRMTRDVAPRLGCKKPSLIHSKFFPGLE 477
            RTP    +  IVKGA    L      TT    +S+ +R  +T+   +  +PT  +   +       +  N P  VF + R  A   Y+ +  +   +P ++ E+ +  + +   +     V+ STG +  I     KF   KK+L +  + V  P E  PA+    K   E +PS   +  L    V                      G+  Q +TPW+V+  +  IDY+KL+K FG   IT  L+SRIE LT ++ H  LRRG+FFSHRDL  LLD  EK                    G  FYLYTGRGPSSE+LH+GHL+PF FT+YLQ+ FDVPLVIQLTDDEKF FK  L LEE HRLA+ENAKD+IACGFD  KTFIFSDL YIQHLYP +L+IQK VT+NQ RG+FGF ESD+ GK AFPAVQAAPSFP++FP+   G  D+ CLIP AIDQD YFRMTRDVAPRLG  KP+LIHS+F P L+
Sbjct:   87 RTPFQDAALSIVKGAACIALSLKTARTTQNVKFSKDNRCSITLGPIEV-EPTAAEEALLKTLTKQKIEENCPFRVFTIPRDLATRMYQESYLDEFGIPANVKEVRLVVLPEWNINANMYPVLKSTGQIADIQFESVKFNEEKKQLCLSLHVVPGPDEQSPALAEEEKLGPEEIPSRAQV--LPPSGVEGDD---------------FGEGDDDQTITPWDVQAAEGGIDYNKLLKKFGCSSITPELISRIEALTGKRAHHLLRRGIFFSHRDLNLLLDSLEKRQARLASGKREDGPGSEGLSGPGFYLYTGRGPSSEALHIGHLVPFMFTKYLQDVFDVPLVIQLTDDEKFLFKDSLTLEETHRLAFENAKDIIACGFDPDKTFIFSDLSYIQHLYPVILEIQKKVTYNQVRGLFGFVESDNVGKSAFPAVQAAPSFPTAFPMIFGGKKDVRCLIPQAIDQDPYFRMTRDVAPRLGLLKPALIHSRFIPALQ 560

The following BLAST results are available for this feature:

BLAST of EWM21129.1 vs. NCBI_GenBank

BLAST of EWM21129.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10


Match Name	E-value	Identity	Description
gi\|585101196\|gb\|EWM21129.1\|	0.000e+0	100.00	tryptophanyl-trna cytoplasmic [Nannochloropsis gad...	[more]
gi\|299472643\|emb\|CBN78295.1\|	0.000e+0	59.21	Tryptophan--tRNA ligase [Ectocarpus siliculosus]	[more]
gi\|873226335\|emb\|CEM21761.1\|	3.792e-163	52.47	unnamed protein product [Vitrella brassicaformis C...	[more]
gi\|294952951\|ref\|XP_002787529.1\|	3.832e-153	51.79	IFP53, putative [Perkinsus marinus ATCC 50983] >gi...	[more]
gi\|294871822\|ref\|XP_002766059.1\|	4.941e-144	51.47	IFP53, putative [Perkinsus marinus ATCC 50983] >gi...	[more]
gi\|294941734\|ref\|XP_002783213.1\|	1.927e-142	51.14	IFP53, putative [Perkinsus marinus ATCC 50983] >gi...	[more]
gi\|401401570\|ref\|XP_003881044.1\|	1.067e-139	46.76	tryptophanyl-tRNAsynthetase,related [Neospora cani...	[more]
gi\|820700112\|tpe\|CEL75845.1\|	1.711e-133	46.14	TPA: tryptophanyl-tRNA synthetase, putative [Toxop...	[more]
gi\|672573471\|gb\|KFH12174.1\|	1.963e-132	46.14	tryptophanyl-tRNA synthetase [Toxoplasma gondii MA...	[more]
gi\|523573065\|gb\|EPR59882.1\|	2.487e-132	46.14	tryptophanyl-tRNA synthetase (TrpRS2) [Toxoplasma ...	[more]

Relationships

This CDS is a part of the following mRNA feature(s):


Feature Name	Unique Name	Species	Type
rna9574	rna9574	Nannochloropsis gaditana (N. gaditana B-31)	mRNA

Sequences

Synonyms

Publications