EWM21750.1, cds8920 (CDS) Nannochloropsis gaditana

BLAST of EWM21750.1 vs. NCBI_GenBank
Match: gi|585102082|gb|EWM21750.1| (dihydroxy-3-keto-5-methylthiopentene dioxygenase [Nannochloropsis gaditana])

HSP 1 Score: 462.225 bits (1188), Expect = 5.797e-165
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0

Query:    1 MQSFQAFVAVYSAPAHRSIMLDAWYMDDDTTMDQREEHRLKPNKPATLEDLQTLGVLHWTFDADSYEDSPEFAAMRAERGYDYEDTCTVSRETMPNYEEKIKSFYEEHIHSDEEIRYCLDGSGYFDVRDGQDRWIRIKVEKNDLIVLPEGIYHRFTLDNKNYIKALRLFKGVPIWTPYNRCVIRAELSSAAAANGRMRGFNKFCFSAKNPHNVAESQA 218
            MQSFQAFVAVYSAPAHRSIMLDAWYMDDDTTMDQREEHRLKPNKPATLEDLQTLGVLHWTFDADSYEDSPEFAAMRAERGYDYEDTCTVSRETMPNYEEKIKSFYEEHIHSDEEIRYCLDGSGYFDVRDGQDRWIRIKVEKNDLIVLPEGIYHRFTLDNKNYIKALRLFKGVPIWTPYNRCVIRAELSSAAAANGRMRGFNKFCFSAKNPHNVAESQA
Sbjct:    1 MQSFQAFVAVYSAPAHRSIMLDAWYMDDDTTMDQREEHRLKPNKPATLEDLQTLGVLHWTFDADSYEDSPEFAAMRAERGYDYEDTCTVSRETMPNYEEKIKSFYEEHIHSDEEIRYCLDGSGYFDVRDGQDRWIRIKVEKNDLIVLPEGIYHRFTLDNKNYIKALRLFKGVPIWTPYNRCVIRAELSSAAAANGRMRGFNKFCFSAKNPHNVAESQA 218          

BLAST of EWM21750.1 vs. NCBI_GenBank
Match: gi|553191094|ref|XP_005855319.1| (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase [Nannochloropsis gaditana CCMP526] >gi|422293738|gb|EKU21038.1| 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 346.28 bits (887), Expect = 4.845e-117
Identity = 161/170 (94.71%), Postives = 162/170 (95.29%), Query Frame = 0

Query:   20 MLDAWYMDDDTTMDQREEHRLKPNKPATLEDLQTLGVLHWTFDADSYEDSPEFAAMRAERGYDYEDTCTVSRETMPNYEEKIKSFYEEHIHSDEEIRYCLDGSGYFDVRDGQDRWIRIKVEKNDLIVLPEGIYHRFTLDNKNYIKALRLFKGVPIWTPYNRCVIRAELSS 189
            MLDAWYMDDDTTMDQREEHRLKPNKPATLEDLQTLGVLHWTFDADSY+DSPEFAAMRAERGYDYEDTCTVSRETMPNYEEKIKSFYEEHIHSDEEIRYCLDGSGYFDVRDGQDRWIRIKVEKNDLIVLPEGIYHRFTLDNKNYIKALRLFKGVPIWTPYN        SS
Sbjct:    1 MLDAWYMDDDTTMDQREEHRLKPNKPATLEDLQTLGVLHWTFDADSYKDSPEFAAMRAERGYDYEDTCTVSRETMPNYEEKIKSFYEEHIHSDEEIRYCLDGSGYFDVRDGQDRWIRIKVEKNDLIVLPEGIYHRFTLDNKNYIKALRLFKGVPIWTPYNSTSTHLITSS 170          

BLAST of EWM21750.1 vs. NCBI_GenBank
Match: gi|632972969|ref|XP_007902920.1| (PREDICTED: 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase [Callorhinchus milii])

HSP 1 Score: 229.18 bits (583), Expect = 2.081e-73
Identity = 102/161 (63.35%), Postives = 127/161 (78.88%), Query Frame = 0

Query:   20 MLDAWYMDDDTTMDQREEHRLKPNKPATLEDLQTLGVLHWTFDADSYEDSPEFAAMRAERGYDYEDTCTVSRETMPNYEEKIKSFYEEHIHSDEEIRYCLDGSGYFDVRDGQDRWIRIKVEKNDLIVLPEGIYHRFTLDNKNYIKALRLFKGVPIWTPYNR 180
            ML AWYMDD +  DQR+ H+L+PNKP +L+ L+ LGVL+W  +AD YED PE   +R ER Y + D  T+ ++T+PNYEEKIKSF+EEHIH DEEIRY +DGSGYFDVRD +++WIRI ++K DLI LP GIYHRFTLD+ NY KA+RLF G P+WTPY R
Sbjct:    1 MLQAWYMDD-SAEDQRKPHKLEPNKPVSLQQLEPLGVLYWKLNADKYEDDPELQKIRKERNYSWMDIITIHKDTLPNYEEKIKSFFEEHIHLDEEIRYIIDGSGYFDVRDKEEQWIRIAMQKGDLITLPAGIYHRFTLDDNNYTKAMRLFVGDPVWTPYAR 160          

BLAST of EWM21750.1 vs. NCBI_GenBank
Match: gi|971514206|dbj|GAQ83993.1| (Uncharacterized conserved protein [Klebsormidium nitens])

HSP 1 Score: 230.72 bits (587), Expect = 2.236e-73
Identity = 105/161 (65.22%), Postives = 123/161 (76.40%), Query Frame = 0

Query:   22 DAWYMDDDTTMDQREEHRLKPNKPATLEDLQTLGVLHWTFDADSYEDSPEFAAMRAERGYDYEDTCTVSRETMPNYEEKIKSFYEEHIHSDEEIRYCLDGSGYFDVRDGQDRWIRIKVEKNDLIVLPEGIYHRFTLDNKNYIKALRLFKGVPIWTPYNRCV 182
            +AWYMDD +  DQR  H+L+PN+P + E L  LGV+HW  DAD YE  PE   +R +R Y Y+D  TVS + +PNYE KIKSF+EEHIH+DEEIRYCLDGSGYFDVRD  D+WIRI V K D+IVLP G+YHRFTLD  NYIKALRLF G P+WTP NR V
Sbjct:   35 EAWYMDD-SDADQRWSHKLEPNQPVSREQLAELGVVHWVLDADKYETDPELQQIRKDRNYSYQDLITVSPDKLPNYEVKIKSFFEEHIHTDEEIRYCLDGSGYFDVRDANDKWIRILVRKGDMIVLPAGMYHRFTLDENNYIKALRLFVGEPVWTPINRPV 194          

BLAST of EWM21750.1 vs. NCBI_GenBank
Match: gi|1211446580|ref|XP_021509242.1| (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase [Meriones unguiculatus] >gi|1211446590|ref|XP_021509247.1| 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase [Meriones unguiculatus])

HSP 1 Score: 227.254 bits (578), Expect = 2.758e-72
Identity = 100/163 (61.35%), Postives = 127/163 (77.91%), Query Frame = 0

Query:   18 SIMLDAWYMDDDTTMDQREEHRLKPNKPATLEDLQTLGVLHWTFDADSYEDSPEFAAMRAERGYDYEDTCTVSRETMPNYEEKIKSFYEEHIHSDEEIRYCLDGSGYFDVRDGQDRWIRIKVEKNDLIVLPEGIYHRFTLDNKNYIKALRLFKGVPIWTPYNR 180
            + M+ AWYMD+ +T D R  HR +P++P  LE L+TLGVL+W  DAD YE+ PE   +R ER Y + D  T+ ++T+PNYEEKIK F+EEH+H DEEIRY L+GSGYFD+RD +D+WIRI +EK D+I LP GIYHRFTLD KNY+KA+RLF G P+WTPYNR
Sbjct:   25 TTMVQAWYMDE-STADPRMPHRAQPDRPVGLEQLRTLGVLYWKLDADKYENDPELEKIRRERNYSWMDIITICKDTLPNYEEKIKMFFEEHLHLDEEIRYILEGSGYFDIRDKEDKWIRISMEKGDMITLPAGIYHRFTLDEKNYVKAMRLFVGEPVWTPYNR 186          

BLAST of EWM21750.1 vs. NCBI_GenBank
Match: gi|19527270|ref|NP_598813.1| (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase [Mus musculus] >gi|78099011|sp|Q99JT9.1|MTND_MOUSE RecName: Full=1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase; AltName: Full=Acireductone dioxygenase (Fe(2+)-requiring); Short=ARD; Short=Fe-ARD; AltName: Full=Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1; Short=MTCBP-1 >gi|1004172041|pdb|5I8S|A Chain A, Structure Of Mouse Acireductone Dioxygenase With Ni2+ Ion And Pentanoic Acid In The Active Site >gi|1004172042|pdb|5I8T|A Chain A, Structure Of Mouse Acireductone Dioxygenase With Ni2+ Ion And D-lactic Acid In The Active Site >gi|1004172043|pdb|5I8Y|A Chain A, Strcuture Of Mouse Acireductone Dioxygenase Bound To Co2+ And 2-keto- 4-(methylthio)-butyric Acid >gi|1004172044|pdb|5I91|A Chain A, Structure Of Mouse Acirecutone Dioxygenase With To Ni2+ And 2-keto-4- (methylthio)-butyric Acid In The Active Site >gi|1004172045|pdb|5I93|A Chain A, Structure Of Mouse Acireductone Dioxygenase With Ni2+ And 2- Ketopentanoic Acid In The Active Site >gi|13543034|gb|AAH05695.1| Adi1 protein [Mus musculus] >gi|74219077|dbj|BAE26682.1| unnamed protein product [Mus musculus])

HSP 1 Score: 226.483 bits (576), Expect = 2.868e-72
Identity = 100/161 (62.11%), Postives = 127/161 (78.88%), Query Frame = 0

Query:   20 MLDAWYMDDDTTMDQREEHRLKPNKPATLEDLQTLGVLHWTFDADSYEDSPEFAAMRAERGYDYEDTCTVSRETMPNYEEKIKSFYEEHIHSDEEIRYCLDGSGYFDVRDGQDRWIRIKVEKNDLIVLPEGIYHRFTLDNKNYIKALRLFKGVPIWTPYNR 180
            M+ AWYMD+ +T D R+ HR +P++P +LE L+TLGVL+W  DAD YE+ PE   +R  R Y + D  T+ ++T+PNYEEKIK F+EEH+H DEEIRY L+GSGYFDVRD +D+WIRI +EK D+I LP GIYHRFTLD KNY+KA+RLF G P+WTPYNR
Sbjct:    1 MVQAWYMDE-STADPRKPHRAQPDRPVSLEQLRTLGVLYWKLDADKYENDPELEKIRKMRNYSWMDIITICKDTLPNYEEKIKMFFEEHLHLDEEIRYILEGSGYFDVRDKEDKWIRISMEKGDMITLPAGIYHRFTLDEKNYVKAMRLFVGEPVWTPYNR 160          

BLAST of EWM21750.1 vs. NCBI_GenBank
Match: gi|1195682460|ref|XP_021034707.1| (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase [Mus caroli])

HSP 1 Score: 226.483 bits (576), Expect = 3.063e-72
Identity = 100/161 (62.11%), Postives = 127/161 (78.88%), Query Frame = 0

Query:   20 MLDAWYMDDDTTMDQREEHRLKPNKPATLEDLQTLGVLHWTFDADSYEDSPEFAAMRAERGYDYEDTCTVSRETMPNYEEKIKSFYEEHIHSDEEIRYCLDGSGYFDVRDGQDRWIRIKVEKNDLIVLPEGIYHRFTLDNKNYIKALRLFKGVPIWTPYNR 180
            M+ AWYMD+ +T D R+ HR +P++P +LE L+TLGVL+W  DAD YE+ PE   +R  R Y + D  T+ ++T+PNYEEKIK F+EEH+H DEEIRY L+GSGYFDVRD +D+WIRI +EK D+I LP GIYHRFTLD KNY+KA+RLF G P+WTPYNR
Sbjct:    1 MVQAWYMDE-STADPRKPHRAQPDRPVSLEQLRTLGVLYWKLDADKYENDPELEKIRKMRNYSWMDIITICKDTLPNYEEKIKMFFEEHLHLDEEIRYILEGSGYFDVRDKEDKWIRISMEKGDMITLPAGIYHRFTLDEKNYVKAMRLFVGEPVWTPYNR 160          

BLAST of EWM21750.1 vs. NCBI_GenBank
Match: gi|1195554169|ref|XP_021057984.1| (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase [Mus pahari])

HSP 1 Score: 226.098 bits (575), Expect = 3.569e-72
Identity = 100/161 (62.11%), Postives = 127/161 (78.88%), Query Frame = 0

Query:   20 MLDAWYMDDDTTMDQREEHRLKPNKPATLEDLQTLGVLHWTFDADSYEDSPEFAAMRAERGYDYEDTCTVSRETMPNYEEKIKSFYEEHIHSDEEIRYCLDGSGYFDVRDGQDRWIRIKVEKNDLIVLPEGIYHRFTLDNKNYIKALRLFKGVPIWTPYNR 180
            M+ AWYMD+ +T D R+ HR +P++P +LE L+TLGVL+W  DAD YE+ PE   +R  R Y + D  T+ ++T+PNYEEKIK F+EEH+H DEEIRY L+GSGYFDVRD +D+WIRI +EK D+I LP GIYHRFTLD KNY+KA+RLF G P+WTPYNR
Sbjct:    1 MVQAWYMDE-STADPRKPHRAQPDRPVSLEQLRTLGVLYWKLDADKYENDPELEKIRKMRNYSWMDIITICKDTLPNYEEKIKMFFEEHLHLDEEIRYILEGSGYFDVRDKEDKWIRISMEKGDMITLPAGIYHRFTLDEKNYVKAMRLFVGEPVWTPYNR 160          

BLAST of EWM21750.1 vs. NCBI_GenBank
Match: gi|302765409|ref|XP_002966125.1| (hypothetical protein SELMODRAFT_230764 [Selaginella moellendorffii] >gi|300165545|gb|EFJ32152.1| hypothetical protein SELMODRAFT_230764 [Selaginella moellendorffii])

HSP 1 Score: 224.557 bits (571), Expect = 1.383e-71
Identity = 104/160 (65.00%), Postives = 123/160 (76.88%), Query Frame = 0

Query:   21 LDAWYMDDDTTMDQREEHRLKPNKPATLEDLQTLGVLHWTFDADSYEDSPEFAAMRAERGYDYEDTCTVSRETMPNYEEKIKSFYEEHIHSDEEIRYCLDGSGYFDVRDGQDRWIRIKVEKNDLIVLPEGIYHRFTLDNKNYIKALRLFKGVPIWTPYNR 180
            ++AWYMDD ++ DQR+ H L P +  + E L  LGVLHW  DAD YE   +   +R +RGY Y+D  TVS E +PNYE+KI+SF+EEHIH DEEIRYCLDGSGYFDVRD  DRWIRI V+K D+IVLP G YHRFTLDN NYIKA+RLF G P+WTPYNR
Sbjct:    1 MEAWYMDD-SSEDQRQPHHLNPVQLVSHEKLAALGVLHWNLDADRYETDSKLQEIRKKRGYSYQDIITVSPEKLPNYEQKIRSFFEEHIHLDEEIRYCLDGSGYFDVRDLGDRWIRIWVKKGDMIVLPAGSYHRFTLDNSNYIKAMRLFVGEPVWTPYNR 159          

BLAST of EWM21750.1 vs. NCBI_GenBank
Match: gi|553727084|ref|NP_001272956.1| (1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase [Loxodonta africana])

HSP 1 Score: 224.172 bits (570), Expect = 2.339e-71
Identity = 100/161 (62.11%), Postives = 126/161 (78.26%), Query Frame = 0

Query:   20 MLDAWYMDDDTTMDQREEHRLKPNKPATLEDLQTLGVLHWTFDADSYEDSPEFAAMRAERGYDYEDTCTVSRETMPNYEEKIKSFYEEHIHSDEEIRYCLDGSGYFDVRDGQDRWIRIKVEKNDLIVLPEGIYHRFTLDNKNYIKALRLFKGVPIWTPYNR 180
            M++AWYMD+ +  DQR+ HRL+P++P  LE L+ LGV +W  DAD YE+ PE   +R ER Y + D  T+S++ +PNYEEKIK FYEEH+H D+EIRY LDGSGYFDVRD +D+WIRI +EK D+I LP GIYHRFTLD KNY+KA+RLF G P+WT YNR
Sbjct:    1 MVEAWYMDE-SADDQRKPHRLEPDRPVGLEQLRQLGVFYWKLDADKYENDPELEKIRKERNYSWTDIITISKDKLPNYEEKIKMFYEEHLHLDDEIRYILDGSGYFDVRDKEDKWIRIFMEKGDMITLPAGIYHRFTLDEKNYVKAMRLFVGEPVWTAYNR 160