EWM21776.1, cds8896 (CDS) Nannochloropsis gaditana

BLAST of EWM21776.1 vs. NCBI_GenBank
Match: gi|585102111|gb|EWM21776.1| (Ion transport [Nannochloropsis gaditana])

HSP 1 Score: 1835.08 bits (4752), Expect = 0.000e+0
Identity = 897/897 (100.00%), Postives = 897/897 (100.00%), Query Frame = 0

Query:    1 MATAPLTRGEGQHEPDGYPYQLHNFEDTVQKAVFCIKDGLDRLIRFHPVVGSPWANWAHRQRLRTVGYILPWLAIWTLILLTFIETPMWCLRDPSTCDPRSSQSELYPRVTSRFVGRAPALTTEFIALSVLAVDYILQIIAEGLLVVTCRPWARGFWHQMLLGASFLSLVISALFPSPWTFRVAPFLRILFLATYSRRLRQEMQLIVRILPQFGKALVLLLSFFLVYAILGLSLFSSTSKNKADAQEAALYFPNFRGAITSVWNLFLYWTFPNMMVLRDPETGKYLPPNVVLTLWVFSFMIIVIYFVLNLALAIVVDVYNEQNREREKRRKMDRQVSMAQAFSLLAVPSGLPPHGSSGQVEAWAAVEDSLMMPATNDPFFRPLASVARSSFVGASASMSTPTAVSSPALPSSTAPVQAVPGHVMRQALLLLSTFSSVPRLTAHTLDLLLGNLTKGDDGWVRKASFMRLPDLLAAVSRKQNHRQPGMHRHHYHRRQQKHHGERTIAQGAYVHAGAGGEEGGEDEEEDGGGEEDEGEGRMRFVDLWCPRLASSRIFAYVSVFVQSAFLEYFIDAVIIANLALAIAANGNGAPPSSPGPTPAPDLTSTTVRTLFTMGAEFHGAVEEGAASKETLLSHRDFSFMAAGIFTLEAVAKLFVYGWKQYVASYMNLFDATLTLLTVGGAIYEVVGSCGGTGHQGSQFHQPFLVFELLRILRLFRALMAIPQFRSTGHAFLKILPSASSLFLNLFAVTFLFAACGLEIYGGLVNTDPARPQLSVLTPTYYYENGLFLFNFNDLGGGFIILVEILIDSSLIQPFYEAFGALVEGNGAGARVGTLIFFVLYYCIGQLVVLNIVVAFILDAFMYESEKEEEGLEENRASRRAMRRQPEGGELSREKGSL 897
            MATAPLTRGEGQHEPDGYPYQLHNFEDTVQKAVFCIKDGLDRLIRFHPVVGSPWANWAHRQRLRTVGYILPWLAIWTLILLTFIETPMWCLRDPSTCDPRSSQSELYPRVTSRFVGRAPALTTEFIALSVLAVDYILQIIAEGLLVVTCRPWARGFWHQMLLGASFLSLVISALFPSPWTFRVAPFLRILFLATYSRRLRQEMQLIVRILPQFGKALVLLLSFFLVYAILGLSLFSSTSKNKADAQEAALYFPNFRGAITSVWNLFLYWTFPNMMVLRDPETGKYLPPNVVLTLWVFSFMIIVIYFVLNLALAIVVDVYNEQNREREKRRKMDRQVSMAQAFSLLAVPSGLPPHGSSGQVEAWAAVEDSLMMPATNDPFFRPLASVARSSFVGASASMSTPTAVSSPALPSSTAPVQAVPGHVMRQALLLLSTFSSVPRLTAHTLDLLLGNLTKGDDGWVRKASFMRLPDLLAAVSRKQNHRQPGMHRHHYHRRQQKHHGERTIAQGAYVHAGAGGEEGGEDEEEDGGGEEDEGEGRMRFVDLWCPRLASSRIFAYVSVFVQSAFLEYFIDAVIIANLALAIAANGNGAPPSSPGPTPAPDLTSTTVRTLFTMGAEFHGAVEEGAASKETLLSHRDFSFMAAGIFTLEAVAKLFVYGWKQYVASYMNLFDATLTLLTVGGAIYEVVGSCGGTGHQGSQFHQPFLVFELLRILRLFRALMAIPQFRSTGHAFLKILPSASSLFLNLFAVTFLFAACGLEIYGGLVNTDPARPQLSVLTPTYYYENGLFLFNFNDLGGGFIILVEILIDSSLIQPFYEAFGALVEGNGAGARVGTLIFFVLYYCIGQLVVLNIVVAFILDAFMYESEKEEEGLEENRASRRAMRRQPEGGELSREKGSL
Sbjct:    1 MATAPLTRGEGQHEPDGYPYQLHNFEDTVQKAVFCIKDGLDRLIRFHPVVGSPWANWAHRQRLRTVGYILPWLAIWTLILLTFIETPMWCLRDPSTCDPRSSQSELYPRVTSRFVGRAPALTTEFIALSVLAVDYILQIIAEGLLVVTCRPWARGFWHQMLLGASFLSLVISALFPSPWTFRVAPFLRILFLATYSRRLRQEMQLIVRILPQFGKALVLLLSFFLVYAILGLSLFSSTSKNKADAQEAALYFPNFRGAITSVWNLFLYWTFPNMMVLRDPETGKYLPPNVVLTLWVFSFMIIVIYFVLNLALAIVVDVYNEQNREREKRRKMDRQVSMAQAFSLLAVPSGLPPHGSSGQVEAWAAVEDSLMMPATNDPFFRPLASVARSSFVGASASMSTPTAVSSPALPSSTAPVQAVPGHVMRQALLLLSTFSSVPRLTAHTLDLLLGNLTKGDDGWVRKASFMRLPDLLAAVSRKQNHRQPGMHRHHYHRRQQKHHGERTIAQGAYVHAGAGGEEGGEDEEEDGGGEEDEGEGRMRFVDLWCPRLASSRIFAYVSVFVQSAFLEYFIDAVIIANLALAIAANGNGAPPSSPGPTPAPDLTSTTVRTLFTMGAEFHGAVEEGAASKETLLSHRDFSFMAAGIFTLEAVAKLFVYGWKQYVASYMNLFDATLTLLTVGGAIYEVVGSCGGTGHQGSQFHQPFLVFELLRILRLFRALMAIPQFRSTGHAFLKILPSASSLFLNLFAVTFLFAACGLEIYGGLVNTDPARPQLSVLTPTYYYENGLFLFNFNDLGGGFIILVEILIDSSLIQPFYEAFGALVEGNGAGARVGTLIFFVLYYCIGQLVVLNIVVAFILDAFMYESEKEEEGLEENRASRRAMRRQPEGGELSREKGSL 897          

BLAST of EWM21776.1 vs. NCBI_GenBank
Match: gi|219126201|ref|XP_002183351.1| (predicted protein, partial [Phaeodactylum tricornutum CCAP 1055/1] >gi|217405107|gb|EEC45051.1| predicted protein, partial [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 113.62 bits (283), Expect = 1.682e-22
Identity = 102/360 (28.33%), Postives = 161/360 (44.72%), Query Frame = 0

Query:  540 FVDLWCPRLASSRIFAYVSVFVQSAFLEYFIDAVIIANLALAIAANGNGAPP-SSPGPTPAPDLTSTTVRTLFTMGAEFHGAVEEGAASKETLLSHRDFSFMAAGIFTLEAVAKLFVYGWKQYVASYMNLFDATLTLLTVGGAIYEVVGSCGGTGHQGSQFHQPFLV--FELLRILRLFRALMAIPQFRSTGHAFLKILPSASSLFLNLFAVTFLFAACGLEIYGGLVNTDPARPQLSVLTPTYYYENGLFLFNFNDLGGGFIILVEILIDSSLIQPFYEAFGALVEGNGAGARVG---------TLIFFVLYYCIGQLVVLNIVVAFILDAF-----MYESEKEEEGLEENRASRRAMR 882
            FV+L  P++  S ++      V+S   EY IDA+++ N   A+       P  S       P     ++ T++           EG  S  T+            I+ LE V K+ V GW+ Y  SY N+FD T+T+L V  +            +  ++F    L+    + R+LRL R L A+ +F+  G   ++ILP+ASS  + LF + + F+A G+ +YGGL+  DPA     +L  T + EN  +  NFND+  G  +L                F  LV  N     VG            FF+ ++  G ++V N+V+AFI++AF     +Y    +EE + +  A  R  R
Sbjct:  225 FVELRLPKIFHSSLYQTFCTVVKSNLFEYSIDAILVMN---AVVIGIQSYPELSGQAVQIDPKYWDGSIDTIW-----------EGVESVFTV------------IYALEVVVKVLVLGWRAYTESYKNVFDFTITILAVISS---------SIVYYPNEFSDSRLIRMIVMARVLRLIRLLTAMKRFQLIGIISVEILPAASSALMVLFCIMYFFSALGMHLYGGLITRDPANSLAYLLLGTDFSENDYWANNFNDMISGMNVL----------------FNMLVVNNWTECEVGFEATTQEKWVRFFFLSFHVCGVILVNNLVIAFIINAFFEELAIYRERTDEEIVGDGEAVIRNRR 533          

BLAST of EWM21776.1 vs. NCBI_GenBank
Match: gi|1072232457|gb|OEU17188.1| (hypothetical protein FRACYDRAFT_225157 [Fragilariopsis cylindrus CCMP1102])

HSP 1 Score: 110.923 bits (276), Expect = 1.243e-21
Identity = 96/347 (27.67%), Postives = 162/347 (46.69%), Query Frame = 0

Query:  540 FVDLWCPRLASSRIFAYVSVFVQSAFLEYFIDAVIIANLALAIAANGNGAPPSSPGPTPAPDLTSTTVRTLFTMGAEFHGAVEEGAASKETLLSHRDFSFMAAGIFTLEAVAKLFVYGWKQYVASYMNLFDATLTLLTVGGAIYEVVGSCGGTGHQGSQFHQPFLVFELLRILRLFRALMAIPQFRSTGHAFLKILPSASSLFLNLFAVTFLFAACGLEIYGGLVNTDPARP-QLSVLTPTYYYENGLFLFNFNDLGGGFIILVEILIDSSLIQPFYEAFGALVEGNGAGARVG---------TLIFFVLYYCIGQLVVLNIVVAFILDAFMYESEK--EEEGLEEN 874
            FV++  PR+  S  +  +  FV+S   EY +D V++ N    I A  +    S    T  P  +   + T++ +               ET+ +          ++ LEA+ K+ V GWK+Y  S  N+FD ++TL+ V  + Y    +     +  S   Q F+V  + R+LRL R L A+  F+  G   + I+P+ASS+F+ LF V + FA+ G+ +YGGL+  DP  P   ++L    + +N  +  NFND+  G  +L                F  +V  N     +G           IFF  ++ +G +V+ N+V +FI++A+  + +   +  G EEN
Sbjct:  194 FVEVRFPRVNQSNWYQGLCKFVKSKGFEYALDIVLVLNAV--IIAFQDYPMLSGQDVTRDPHYSDGYIDTIWEL--------------METIFTV---------VYVLEAILKITVEGWKRYSESPRNMFDFSITLMAVLASAYVYYPN----AYSNSALIQ-FVV--MARVLRLGRILFAMKAFQMFGTITIDIIPAASSVFMILFFVEYFFASMGVLLYGGLITRDPKNPLSYALLEADDFVDNKYWANNFNDMVSGMNVL----------------FNLVVVNNWTECEIGFEYVTGAKWVRIFFFCFHLMGVIVLSNVVTSFIINAYFQQMKTLVQRLGWEEN 492          

BLAST of EWM21776.1 vs. NCBI_GenBank
Match: gi|1072925663|ref|XP_018473520.1| (PREDICTED: two pore calcium channel protein 1-like [Raphanus sativus])

HSP 1 Score: 111.309 bits (277), Expect = 2.176e-21
Identity = 79/274 (28.83%), Postives = 135/274 (49.27%), Query Frame = 0

Query:  621 VEEGAASKETLLSHRDFSFMAAGIFTLEAVAKLFVYGWKQYVASYMNLFDATLTLLTVGGAIYEVVGSCGGTGHQGSQFHQPFLVFELLRILRLFRALMAIPQFRSTGHAFLKILPSASSLFLNLFAVTFLFAACGLEIYGGLVNTDPARPQLSVLTPTYYYENGLFLFNFNDLGGGFIILVEILIDSSLIQPFYEAFGALVEGNGAGARVGTLIFFVLYYCIGQLVVLNIVVAFILDAFMYESEKEEEGLEENRASRRAMRRQPEGGELSREK 894
            +EE +A K   ++     F+   I+ +E   K++ YG++ Y     N FD  +T + V G     +     T     ++ +  L   L R+LRL R LM + Q+R+    F+ ++PS       +F V  ++ + G++++GGLVN    +     L  T   EN   LFNFND   G + L  +L+  +  Q + E++  L           ++ +FV +Y I  L++LN++VAF+L+AF  E + EEE   + + S+    R+P  G  SR +
Sbjct:  455 IEESSAQKPWQVAE----FVFGWIYVMEMALKIYSYGFENYWRDGQNRFDFLVTWVIVIGETATFITPDENTFFSNGEWIRYLL---LARMLRLIRLLMHVQQYRAFIATFITLIPSLMPYLGTIFCVLCIYCSIGVQVFGGLVNAGNKK-----LFETELAENDYLLFNFNDYPNGMVTLFNLLVMGNW-QAWMESYKDLT------GTWWSITYFVSFYIITVLLLLNLIVAFVLEAFFTELDLEEEEKCQGQDSQERRNRRPYAGSKSRSQ 709          

BLAST of EWM21776.1 vs. NCBI_GenBank
Match: gi|922432106|ref|XP_013622160.1| (PREDICTED: two pore calcium channel protein 1-like [Brassica oleracea var. oleracea])

HSP 1 Score: 108.612 bits (270), Expect = 1.254e-20
Identity = 78/274 (28.47%), Postives = 135/274 (49.27%), Query Frame = 0

Query:  621 VEEGAASKETLLSHRDFSFMAAGIFTLEAVAKLFVYGWKQYVASYMNLFDATLTLLTVGGAIYEVVGSCGGTGHQGSQFHQPFLVFELLRILRLFRALMAIPQFRSTGHAFLKILPSASSLFLNLFAVTFLFAACGLEIYGGLVNTDPARPQLSVLTPTYYYENGLFLFNFNDLGGGFIILVEILIDSSLIQPFYEAFGALVEGNGAGARVGTLIFFVLYYCIGQLVVLNIVVAFILDAFMYESEKEEEGLEENRASRRAMRRQPEGGELSREK 894
            +EE +A K   ++     F+   I+ +E   K++ YG++ Y     N FD  +T + V G    ++     T     ++ +  L   L R+LRL R LM + Q+R+    F+ ++PS       +F V  ++ + G++++GGLVN    +     L  T   EN   LFNFND   G + L  +L+  +  Q + E++  L           ++ +FV +Y I  L++LN++VAF+L+AF  E + EEE   + + S+    R+   G  SR +
Sbjct:  455 IEESSAQKPWQVAE----FVFGWIYVMEMALKIYSYGFENYWRDGQNRFDFLVTWVIVIGETATLITPDENTFFSNGEWIRYLL---LARMLRLIRLLMHVQQYRAFIATFITLIPSLMPYLGTIFCVLCIYCSIGVQVFGGLVNAGNKK-----LFETELAENDYLLFNFNDYPNGMVTLFNLLVMGNW-QAWMESYKDLT------GTWWSITYFVSFYIITVLLLLNLIVAFVLEAFFTELDLEEEEKCQGQDSKERRNRRRYAGSKSRSQ 709          

BLAST of EWM21776.1 vs. NCBI_GenBank
Match: gi|923747922|ref|XP_013673306.1| (PREDICTED: two pore calcium channel protein 1-like [Brassica napus])

HSP 1 Score: 107.842 bits (268), Expect = 2.374e-20
Identity = 78/274 (28.47%), Postives = 134/274 (48.91%), Query Frame = 0

Query:  621 VEEGAASKETLLSHRDFSFMAAGIFTLEAVAKLFVYGWKQYVASYMNLFDATLTLLTVGGAIYEVVGSCGGTGHQGSQFHQPFLVFELLRILRLFRALMAIPQFRSTGHAFLKILPSASSLFLNLFAVTFLFAACGLEIYGGLVNTDPARPQLSVLTPTYYYENGLFLFNFNDLGGGFIILVEILIDSSLIQPFYEAFGALVEGNGAGARVGTLIFFVLYYCIGQLVVLNIVVAFILDAFMYESEKEEEGLEENRASRRAMRRQPEGGELSREK 894
            +EE +A K   ++     F+   I+ +E   K++ YG++ Y     N FD  +T + V G     +     T     ++ +  L   L R+LRL R LM + Q+R+    F+ ++PS       +F V  ++ + G++++GGLVN    +     L  T   EN   LFNFND   G + L  +L+  +  Q + E++  L           ++ +FV +Y I  L++LN++VAF+L+AF  E + EEE   + + S+    R+   G  SR +
Sbjct:  455 IEESSAQKPWQVAE----FVFGWIYVMEMALKIYSYGFENYWRDGQNRFDFLVTWVIVIGETATFITPDENTFFSNGEWIRYLL---LARMLRLIRLLMHVQQYRAFIATFITLIPSLMPYLGTIFCVLCIYCSIGVQVFGGLVNAGNKK-----LFETELAENDYLLFNFNDYPNGMVTLFNLLVMGNW-QAWMESYKDLT------GTWWSITYFVSFYIITVLLLLNLIVAFVLEAFFTELDLEEEEKCQGQDSKERRNRRRYAGSKSRSQ 709          

BLAST of EWM21776.1 vs. NCBI_GenBank
Match: gi|674872220|emb|CDY61997.1| (BnaA03g57170D [Brassica napus])

HSP 1 Score: 107.842 bits (268), Expect = 2.543e-20
Identity = 78/274 (28.47%), Postives = 134/274 (48.91%), Query Frame = 0

Query:  621 VEEGAASKETLLSHRDFSFMAAGIFTLEAVAKLFVYGWKQYVASYMNLFDATLTLLTVGGAIYEVVGSCGGTGHQGSQFHQPFLVFELLRILRLFRALMAIPQFRSTGHAFLKILPSASSLFLNLFAVTFLFAACGLEIYGGLVNTDPARPQLSVLTPTYYYENGLFLFNFNDLGGGFIILVEILIDSSLIQPFYEAFGALVEGNGAGARVGTLIFFVLYYCIGQLVVLNIVVAFILDAFMYESEKEEEGLEENRASRRAMRRQPEGGELSREK 894
            +EE +A K   ++     F+   I+ +E   K++ YG++ Y     N FD  +T + V G     +     T     ++ +  L   L R+LRL R LM + Q+R+    F+ ++PS       +F V  ++ + G++++GGLVN    +     L  T   EN   LFNFND   G + L  +L+  +  Q + E++  L           ++ +FV +Y I  L++LN++VAF+L+AF  E + EEE   + + S+    R+   G  SR +
Sbjct:  438 IEESSAQKPWQVAE----FVFGWIYVMEMALKIYSYGFENYWRDGQNRFDFLVTWVIVIGETATFITPDENTFFSNGEWIRYLL---LARMLRLIRLLMHVQQYRAFIATFITLIPSLMPYLGTIFCVLCIYCSIGVQVFGGLVNAGNKK-----LFETELAENDYLLFNFNDYPNGMVTLFNLLVMGNW-QAWMESYKDLT------GTWWSITYFVSFYIITVLLLLNLIVAFVLEAFFTELDLEEEEKCQGQDSQERRNRRRYAGSKSRSQ 692          

BLAST of EWM21776.1 vs. NCBI_GenBank
Match: gi|685286821|ref|XP_009134341.1| (PREDICTED: two pore calcium channel protein 1-like [Brassica rapa])

HSP 1 Score: 107.071 bits (266), Expect = 3.977e-20
Identity = 78/274 (28.47%), Postives = 134/274 (48.91%), Query Frame = 0

Query:  621 VEEGAASKETLLSHRDFSFMAAGIFTLEAVAKLFVYGWKQYVASYMNLFDATLTLLTVGGAIYEVVGSCGGTGHQGSQFHQPFLVFELLRILRLFRALMAIPQFRSTGHAFLKILPSASSLFLNLFAVTFLFAACGLEIYGGLVNTDPARPQLSVLTPTYYYENGLFLFNFNDLGGGFIILVEILIDSSLIQPFYEAFGALVEGNGAGARVGTLIFFVLYYCIGQLVVLNIVVAFILDAFMYESEKEEEGLEENRASRRAMRRQPEGGELSREK 894
            +EE +A K   ++     F+   I+ +E   K++ YG++ Y     N FD  +T + V G     +     T     ++ +  L   L R+LRL R LM + Q+R+    F+ ++PS       +F V  ++ + G++++GGLVN    +     L  T   EN   LFNFND   G + L  +L+  +  Q + E++  L           ++ +FV +Y I  L++LN++VAF+L+AF  E + EEE   + + S+    R+   G  SR +
Sbjct:  455 IEESSAQKPWQVAE----FVFGWIYVMEMALKIYSYGFENYWRDGQNRFDFLVTWVIVIGETATFITPDENTFFSNGEWIRYLL---LARMLRLIRLLMHVQQYRAFIATFITLIPSLMPYLGTIFCVLCIYCSIGVQVFGGLVNAGNKK-----LFETELAENDYLLFNFNDYPNGMVTLFNLLVMGNW-QAWMESYKDLT------GTWWSITYFVSFYIITVLLLLNLIVAFVLEAFFTELDLEEEEKCQGQDSQERRNRRRYAGSKSRSQ 709          

HSP 2 Score: 65.0846 bits (157), Expect = 4.964e-7
Identity = 85/347 (24.50%), Postives = 144/347 (41.50%), Query Frame = 0

Query:   70 LPW-LAIWTLILLTFIETPMWCLRDPSTCDPRSSQSELYPRVTSRFVGRAPALTTEFIALSVLAVDYILQIIAEGLLVVTCRPWARGFWHQMLLGASFLSLVISA-------------LFPSPWTF-----RVAPFLRILFLATYSRRLRQEMQLIVRILPQFGKALVLLLSFFLVYAILGLSLFSSTSKNKADAQEAALYFPNFRGAITSVWNLFLYWTFPNMMVLRDPETGKYLPPNVVLTLWVFSFMIIVIYFVLNLALAIVVDVYNEQ------NREREKRRKMDRQVSMAQAFSLLAVPSGLPPHGSSGQVEAWAAVEDSLMMPATNDPFFRPLASVARSSF 391
            L W L  + LILL F E P+WC   P+   P     + Y      ++  A ++  E + L +L V     II EG         +R FW       S L+LV  A             L+ SP  F     RVAP++R++      R LR  + L+  +L  +   L L + F L  + +   +F        D ++    F ++   +  ++ LF     P++ + R  ++ ++     VL      +++I +YFV NL LA+V D + EQ        ++ KRR +++      AF       GL     +G++     ++  L    TN   +R L  +++  F
Sbjct:   72 LIWSLNYFALILLNFFEQPLWCENKPT---PSCKDRDYYYLGELPYLTNAESIIFEVLTLLILLVHTFFPIIYEG---------SRIFW------TSRLNLVKVACVVVLIVDVLVDFLYLSPLAFDFLPFRVAPYVRVIIFILSIRGLRDSLILLSGMLGTYLNILALWMLFLLFASWIAFVMFE-------DTKQGLTVFTSYGATLYQMFILFTTSNNPDVWI-RAYKSSRWSSLFFVL------YVLIGVYFVTNLILAVVYDSFKEQLAKQVIGMDQMKRRMLEK------AF-------GLIDTDRTGEINKEQCIK--LFEQLTN---YRTLPKISKEEF 368          

BLAST of EWM21776.1 vs. NCBI_GenBank
Match: gi|299117413|emb|CBN73916.1| (Similar to Oryza sativa TPC1_ORYSJ Two pore calcium channel protein 1. Protein fragment [Ectocarpus siliculosus])

HSP 1 Score: 106.686 bits (265), Expect = 4.941e-20
Identity = 84/324 (25.93%), Postives = 146/324 (45.06%), Query Frame = 0

Query:  540 FVDLWCPRLASSRIFAYVSVFVQSAFLEYFIDAVIIANLALAIAANGNGAPPSSPGPTPAPDLTSTTVRTLFTMGAEFHGAVEEGAASKETLLSHRDFSFMAAGIFTLEAVAKLFVYGWKQYVASYMNLFDATLTLLTVGGAIYEVVGSCGGTGHQGSQFHQPFLVFELLRILRLFRALMAIPQFRSTGHAFLKILPSASSLFLNLFAVTFLFAACGLEIYGGLVNTDPARPQLSVLTPTYYYENGLFLFNFNDLGGGFIILVEILIDSSLIQPFYEAFGALVEGNGAGARVGTLIFFVLYYCIGQLVVLNIVVAFILDAFMYE 863
            FV+ W P L +S  +  +   ++  + EY +DAV++ N  L +  +             A  L+   V+          G + E  A  +   +    + +   ++ +E   K+ V+GW  Y +   N FD  +TL   GG   E++     T           + F   R+LR+ R ++A+P+ R     F  I P A    + LF   F F   G++++GGLV++DP  P    L  T Y ++  + FNFND+  G   L  +L+    +  ++      V+ +G         FFV +Y +G LV+LN+V+A +L+ F+ +
Sbjct:  222 FVETWLPDLYNSSFWQRLCKIIKHPWFEYGVDAVLMLNALLLLVES-------------AEALSGEAVQR--------DGGISEKNAWLQAEGTDHGLNSLLGWVYVVEMTLKILVFGWNGYWSKNRNRFDGVITL---GGVFTELLLKTT-TISTTLNLQNVVVYFVTFRLLRVARLMVAVPEMRIVMTTFGNIAPEAFQFMMLLFVCGFTFCVLGVQLFGGLVHSDPDSPTNEHLKDTSYGQSNYWAFNFNDMPSGMGTLFCLLV----LNNWFVFVDVFVKASGDNK--WNRWFFVAFYVLGVLVILNLVIAVVLENFIIQ 514          

BLAST of EWM21776.1 vs. NCBI_GenBank
Match: gi|1132605037|pdb|5TUA|A (Chain A, Structure Of A Na+-selective Mutant Of Two-pore Channel From Arabidopsis Thaliana Attpc1)

HSP 1 Score: 106.686 bits (265), Expect = 5.318e-20
Identity = 78/274 (28.47%), Postives = 135/274 (49.27%), Query Frame = 0

Query:  621 VEEGAASKETLLSHRDFSFMAAGIFTLEAVAKLFVYGWKQYVASYMNLFDATLTLLTVGGAIYEVVGSCGGTGHQGSQFHQPFLVFELLRILRLFRALMAIPQFRSTGHAFLKILPSASSLFLNLFAVTFLFAACGLEIYGGLVNTDPARPQLSVLTPTYYYENGLFLFNFNDLGGGFIILVEILIDSSLIQPFYEAFGALVEGNGAGARVGTLIFFVLYYCIGQLVVLNIVVAFILDAFMYESEKEEEGLEENRASRRAMRRQPEGGELSREK 894
            +EE +A K   ++     F+   I+ LE   K++ YG++ Y     N FD  +T + V G     +     T     ++ +  L   L R+LRL R LM + ++R+    F+ ++PS       +F V  ++ + G++++GGLVN    +     L  T   E+   LFNFND   G + L  +L+ ++  Q + E++  L           ++ +FV +Y I  L++LN+VVAF+L+AF  E + EEE   + + S+    R+   G  SR +
Sbjct:  455 IEESSAQKPWQVAE----FVFGWIYVLEMALKIYTYGFENYWREGANRFDFLVTWVIVIGETATFITPDENTFFSNGEWIRYLL---LARMLRLIRLLMNVQRYRAFIATFITLIPSLMPYLGTIFCVLCIYCSIGVQVFGGLVNAGNKK-----LFETELAEDDYLLFNFNDYPNGMVTLFNLLVVNNW-QVWMESYKDLT------GTWWSITYFVSFYVITILLLLNLVVAFVLEAFFTELDLEEEEKCQGQDSQEKRNRRRSAGSKSRSQ 709          

HSP 2 Score: 73.1738 bits (178), Expect = 1.474e-9
Identity = 80/336 (23.81%), Postives = 144/336 (42.86%), Query Frame = 0

Query:   70 LPW-LAIWTLILLTFIETPMWCLRDPSTCDPRSSQSELYPRVTSRFVGRAPALTTEFIALSVLAVDYILQIIAEGLLVVTCRPWARGFWHQML-------LGASFLSLVISALFPSPWTF-----RVAPFLRILFLATYSRRLRQEMQLIVRILPQFGKALVLLLSFFLVYAILGLSLFSSTSKNKADAQEAALYFPNFRGAITSVWNLFLYWTFPNMMVLRDPETGKYLPPNVVLTLWVFSFMIIVIYFVLNLALAIVVDVYNEQ-NREREKRRKMDRQVSMAQAFSLLAVPSGLPPHGSSGQVEAWAAVEDSLMMPATNDPFFRPLASVARSSF 391
            L W L  + L+ L F E P+WC ++P    P     + Y      ++  A ++  E I L++L V     I  EG         +R FW   L       +   F+ +++  L+ SP  F     R+AP++R++      R LR  + L+  +L  +   L L + F L  + +   +F        D Q+    F ++   +  ++ LF     P++ +   P        +V   L+V    +I +YFV NL LA+V D + EQ  ++     +M R++ + +AF       GL     +G+++    ++  L    TN   +R L  +++  F
Sbjct:   72 LIWSLNYFALLFLNFFEQPLWCEKNPK---PSCKDRDYYYLGELPYLTNAESIIYEVITLAILLVHTFFPISYEG---------SRIFWTSRLNLVKVACVVILFVDVLVDFLYLSPLAFDFLPFRIAPYVRVIIFILSIRELRDTLVLLSGMLGTYLNILALWMLFLLFASWIAFVMFE-------DTQQGLTVFTSYGATLYQMFILFTTANNPDVWI---PAYKSSRWSSVFFVLYV----LIGVYFVTNLILAVVYDSFKEQLAKQVSGMDQMKRRM-LEKAF-------GLIDSDKNGEIDKNQCIK--LFEQLTN---YRTLPKISKEEF 368